How do our antibodies stack-up?

Side-by-side comparisons of Bio-Techne and Santa Cruz antibodies

how do our antibodies stack up

SANTA CRUZ WILL NOT SELL POLYCLONAL ANTIBODIES STARTING DEC. 31, 2016: As a result of a groundbreaking USDA ruling, a large number of Santa Cruz polyclonal antibodies will be discontinued starting December 31st.  In light of this news, Bio-Techne is working overtime to support your research and keep your experiments moving forward with minimal to no interruption.  


Get a match & save


THE RISK OF TESTING SANTA CRUZ’S TOP SELLING ANTIBODIES: As part of our internal effort to quell your concerns about switching antibody suppliers, we have begun putting our own antibodies to the test. We picked a handful of Santa Cruz’s most cited antibodies and performed head-to-head comparisons on multiple applications ranging from western blot to flow cytometry. Our decision to test Santa Cruz’s most cited antibodies was risky, but deliberate. We understood that their top selling antibodies were validated by thousands of peer-reviewed publications and heavily relied on by many labs. There was the obvious risk that our antibodies might not stack up against their best sellers.  

OUR COMMITMENT TO YOUR RESEARCH: However, at Bio-Techne, our commitment to your research compelled us to take that risk anyway. We want you to make the most informed decisions about your research, and we felt we could help by providing the data here for your personal review. As part of our commitment, Bio-Techne has started publishing the results of our side-by-side comparisons. The first batch of data can be found below, and we will continue to test our antibodies against equivalent Santa Cruz products.  

SUGGEST A SANTA CRUZ ANTIBODY FOR US TO TEST: If you are looking to change antibody suppliers and would like to suggest a Santa Cruz antibody for our lab to test in a side-by-side comparison, we would welcome your input. Please send us an email with a Santa Cruz Catalog Number and a Target Name for us to test.


Side by side data comparison:



Target

Cat #

Reactivity

Applications

Pubs

WB

Flow

ICC

IHC

53BP1 SC-10911
NB100-304
MAB18772
Hu, Av, Eq, Bv
Hu, Mu, Rt, Fi, Gt, Pm
Hu
WB, IF, ELISA
WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
WB, IHC, ICC
1
248
     
Actin SC-1616
NBP2-25142
NB100-74340
Hu, Mo, Rt
Hu, Mo, Rt
Hu, Mo, Rt
WB, IP, IF, IHC, Flow
WB, ICC, Flow
WB, IP, IF, IHC, Flow
2598
0
14
 
Axl SC-1096
AF154
Mu, Rt, Hu
Hu
WB, IP, IF, IHC-P
WB, Flow, IHC, Block, CyTOF-ready
20
19
     
beta-Actin SC-47778
NBP1-47423
NB600-501
MAB8929
Hu, Mo, Rt
Hu, Mo, Rt
Hu, Mo, Rt
Hu, Mo, Rt
WB, IF, IHC
WB, Flow, ICC, IF
WB, IP, IF, IHC, Flow
WB
1940
0
240
1
 
Caspase-3 SC-7148
NB100-56708
NB100-56112
NB500-210
AF-605-NA
Mu, Rt, Hu, Eq, Ca, Po
Hu, Mu, Rt, Po, ChHa, Ma
Hu, Mu, Rt, Ca, Ge
Hu, Mu, Rt
Hu, Mu
WB, IP, IF, ELISA
WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
WB, Simple Western, IHC, IHC-Fr, IHC-P, IP, ChIP
WB, Simple Western, IHC, IHC-P
WB, Simple Western, IP
521
101
11
17
5
   
CDK2 SC-163
NB100-2721
AF4654
Hu, Mo, Rt
Hu, Mo
Hu, Mo
WB, IF, IP
WB, Flow
WB, IHC, ICC
939
1
0
 
c-Fos SC-52
AF7254
Mu, Rt, Hu, Eq, Fe
Hu
WB, IP, IF, IHC-P, FCM, ELISA
WB
599
     
EGFR SC-03-G
AF231
Mu, Rt, Hu, Eq, Ca, Bv, Po
Hu
WB, IP, IF, IHC-P, ELISA
WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap)
26
6
   
ErbB4 SC-283
AF1131
MAB1131
Mu, Rt, Hu, Av, Eq, Ca, Bv, Po
Hu
Hu
WB, IP, IF, IHC-P, FCM, ELISA
WB, Flow, CyTOF-ready
WB, IHC
154
1
3
     
ERK1 SC-94
AF1940
MAB1940
Mu, Rt, Hu, Ch, Fr, Eq, Ca, Bv, Po, Av
Hu
Hu
WB, IP, IF, IHC-P, FCM, ELISA
WB
WB, IHC, ICC
652

   
ERK2 SC-154
NB110-60506
AF1230
MAB1230
Hu, Mo, Rt
Hu, Mo, Rt
Hu, Mo, Rt
Hu, Mo, Rt
WB, IP, IF, IHC, Flow
WB, ICC, IHC, ELISA
WB, IHC
WB, IHC, ICC
1138
0
0
0
 
GAPDH
SC-25778
SC-47724
NB300-328
NBP1-47339
AF5718
MAB5718
Hu, Mo, Rt
Hu, Mo, Rt
Hu, Mo, Rt
Hu, Mo
Hu, Mo, Rt
Hu, Mo, Rt
WB, IP, IF, IHC
WB, IP, IF, IHC
WB, ICC, IF, IHC
WB, ICC, IF, IHC
WB, IHC
WB, IHC
1177
334
33
4
4
1
   
HIF-1 alpha SC-10790
SC-13515
AF1935
MAB1536
Mu, Hu, Rt, Ca, Bv
Mu, Rt, Hu, Bv, Po
Hu, Mu, Rt
Hu, Mu, Rt
WB, IP, IF, ELISA
WB, IP, IF, IHC-P
WB, SW, IP, ChIP
WB, SW, IP
118
46
12
12
   
IL-1 beta SC-1252
AF-201-NA
AF-401-NA
MAB601
MAB6964
Mu, Rt
Hu
Mu
Hu
Hu
WB, IP, IF, ELISA
WB, ICC, Neut
WB, Simple Western, IHC, ICC, Neut
WB, ELISA(Cap), ICC, Neut
WB, Simple Western, ICC
28
17
58
19
1
     
anti-goat IgG
(H+L) [HRP]
SC-2020
NBP1-74822
HAF109
HAF017
Gt
Gt
Gt
Gt
WB
WB, IHC
WB,
WB
1066
0
13
11
     
anti-rabbit IgG
(H+L) [HRP]
SC-2004
NBP2-30348H
NBP1-75297
HAF008
Rb
Rb
Rb
Rb
WB
WB
WB, IHC, Flow
WB
1692
4
1
12
     
anti-mouse IgG
(H+L) [HRP]
SC-2005
NBP1-75144
HAF007
HAF018
Mo
Mo
Mo
Mo
WB
WB, IHC
WB
WB
1471
0
16
2
     
HA-Tag SC-805
SC-7392
NB600-362
NB600-363
MAB0601
MAB6875
Multi
Multi
Multi
Multi
Multi
Multi
WB, IP, IF
WB,IP, FC, ICC/IF
WB, ICC/IF, IP
WB, ICC/IF, ChIP, IHC, IP
WB, IP, ICC
WB, ICFlow
696
696
11
59
 
0
   
IkB-alpha SC-371
NB100-56507
AF4299
MAB4299
Hu
Hu, Mu, Rt
Hu, Mu
Hu
WB, Flow, ICC/IF, IHC, IP
WB
WB, IP, IF/ICC, IHC, Flow
WB, IHC
139
32
0
1
   
LC3B SC-28266
MAB85581
NBP2-46892
NB100-2220
Hu, Mu, Rt, Eq, Ca, Bv, Po, Av
Hu, Mu, Rt, Po, Bt, Bv, Fi, Xp, Ze
Hu, Mu, Rt, Po, Ba, Bv, Ca, Pm, SyHa, Ze
WB, IP, IF, ELISA
WB, Simple Western
WB, ICC/IF, IHC, IHC-P, IP
WB, Simple Western, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
34
 
2
551
   
Nanog SC-33759
SC-293121
AF1997
AF2729
Hu
Hu
Hu
Mu
WB, IP, IF, ELISA
WB, IP, IF, ELISA
WB, ChIP, ICC
WB, CyTOF-ready, ICC, ICFlow
19
1
51
12
     
Oct-3/4 SC-8628
AF1759
MAB17591
Mu, Rt, Hu, Eq, Po
Hu, Mu
Hu
WB, IP, IF, ELISA
WB, Simple Western, IP, ChIP, ICC
WB, CyTOF-ready, ICC, ICFlow
76
14
1
   
p21 SC-397
NBP2-44525
AF1047
Hu, Mo, Rt
Hu
Hu
WB, IP, IF, IHC
WB, Flow, IHC, IF
WB, IP, IHC, ICC, Flow
1269
0
1
     
p53
SC-126
SC-6243
NBP2-29453
AF1355
MAB1355
Hu
Hu, Mo
Hu, Mk
Hu, Mo, Rt
Hu, Mo, Rt
WB, IP, IF, IHC, Flow
WB, IP, IF, IHC
WB, Flow, IHC, IF, IP
WB, IP, ICC
WB, IP, ICC
1584
901
2
4
3
 
pan Cytokeratin SC-15367
NBP2-29429
Mu, Rt, Hu, Eq, Bv, Po
Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb
WB, IP, IF, IHC-P, ELISA
WB, Flow, ICC/IF, IHC-Fr, IHC-P
25
9
     
RelA/NFkB p65 SC-372
SC-109
NB100-56712
AF5078
MAB5078
Hu
Hu
Hu, Mu, Rt
Hu, Mu
Hu, Mu
WB, IP, IF/ICC,IHC, Flow
ChIP, IP, WB, IHC, IF/ICC, Flow
WB, Flow, IHC
WB, IHC, ICC
WB, ICC
139
390
9
1
0
 
SOX2 SC-17320
SC-365823
AF2018
MAB2018R
Mu, Rt, Hu, Av, Eq, Ca, Bv, Po, Av
Mu, Rt, Hu, Eq, Ca, Bv
Hu, Mu, Rt
Hu, Mu, Rt
WB, IP, IF, IHC-P, ELISA
WB, IP, IF, IHC-P, ELISA
WB, Simple Western, ChIP, ICC
WB, ICFlow
117
7
39
 
   
Survivin SC-17779
NB500-201
MAB886
MAB8861
AF6471
AF886
Mu, Rt, Hu
Hu, Mu, Rt, Ca, Fe, GP, Ha
Hu
Hu
Hu
Hu
WB, IP, IF, IHC-P, ELISA
WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
CyTOF-ready, ICFlow
WB, Simple Western, ICC
WB, Simple Western, Flow, IHC, CyTOF-ready
WB, Simple Western, IHC
139
277
1
 
2
32