Actin Antibody (mAbGEa)

Images

 
Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Western blot analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - Actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB100-74340) at 1:1800 ...read more
Immunocytochemistry/ Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - Actin (mAbGEa) antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) ...read more
Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Western blot analysis of Jurkat and CHO cell lysates using actin antibody [NB100-74340] at 1:100.
Immunocytochemistry/ Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Flow Cytometry: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of HeLa cells using mouse Actin antibody (Orange) and Isotype control Antibody (Blue).
Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - IHC analysis of Actin on human breast cancer tissue using DAB with hematoxylin counterstain
Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Simple Western: Actin Antibody (mAbGEa) [NB100-74340] - Simple Western lane view shows a specific band for Actin in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChHa, Dr, Fu, Pl, Pr, Rb, Sh, Xp, Ye, ZeSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
mAbGEa
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Actin Antibody (mAbGEa) Summary

Immunogen
Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46]
Localization
Cytoplasm > cytoskeleton
Specificity
Reacts with Actin 1, 2, 3, 4, 7, 8, 11 and 12.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:1000
  • Simple Western 1:25
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Actin Antibody (mAbGEa) is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band is seen at ~45 kDa representing Actin. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
A-431 Lysate (NB800-PC12)
HeLa Lysate (NB800-PC1)
MCF-7 Lysate (NB820-59465)
HepG2 Lysate (NBP1-42569)
NTERA-2 Lysate (NBP1-44968)
COS-7 Lysate (L054V2)
PC-12 Lysate (NBP2-13170)
Reviewed Applications
Read 8 Reviews rated 4.1
using
NB100-74340 in the following applications:

Publications
Read Publications using
NB100-74340 in the following applications:

  • WB
    16 publications

Reactivity Notes

Yeast, Xenopus laevis, Drosophila, Zebrafish, Human, Sheep, Mouse, Rat, Bovine, Rabbit, Arabidopsis, Protozoa and Fungi. Chinese hamster data from customer review.Sordaria macrospora reactivity reported in scientific literature (PMID: 24720701)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for Actin Antibody (mAbGEa)

  • ACTA
  • Actin
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB2669
Species: Hu
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NB100-74340
Species: Hu, Mu, Rt, Bv, ChHa, Dr, Fu, Pl, Pr, Rb, Sh, Xp, Ye, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for Actin Antibody (NB100-74340)(19)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Fungi, Plant, Sordaria macrospora, Yeast.

We have publications tested in 1 application: WB.


Filter By Application
WB
(16)
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Filter By Species
Human
(6)
Mouse
(3)
Rat
(1)
Fungi
(2)
Plant
(3)
Sordaria macrospora
(1)
Yeast
(2)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-74340 Applications Species
Yang Q, Wang P, Du X et al. Direct repression of IGF2 is implicated in the anti-angiogenic function of microRNA-210 in human retinal endothelial cells Angiogenesis 2018 Jan 15 [PMID: 29335842] (Human) Human
Luevano-Martinez LA, Forni MF, Peloggia J et al. Calorie restriction promotes cardiolipin biosynthesis and distribution between mitochondrial membranes. Mech. Ageing Dev. 2017 Feb 14 [PMID: 28213011] (WB, Rat) WB Rat
Mana G, Clapero F, Panieri E et al. PPFIA1 drives active alpha5beta1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis. Nat Commun. Nov 23 2016 [PMID: 27876801] (Human) Human
Wang P, Du X, Xiong M et al. Ginsenoside Rd attenuates breast cancer metastasis implicating derepressing microRNA-18a-regulated Smad2 expression Sci Rep Sep 19 2016 [PMID: 27641158] (WB, Human) WB Human
Yang Q, Wang P, Cui J et al. Panax notoginseng saponins attenuate lung cancer growth in part through modulating the level of Met/miR-222 axis. J Ethnopharmacol. Aug 23 2016 [PMID: 27566197] (WB, Human) WB Human
Yang Q, Cui J, Wang P et al. Changes in interconnected pathways implicating microRNAs are associated with the activity of apocynin in attenuating myocardial fibrogenesis. Eur. J. Pharmacol. May 9 2016 [PMID: 27174579] (WB, Mouse) WB Mouse
Lin YJ, Ho TJ, Yeh YC et al. Chinese Herbal Medicine Treatment Improves the Overall Survival Rate of Individuals with Hypertension among Type 2 Diabetes Patients and Modulates In Vitro Smooth Muscle Cell Contractility. PLoS One 2015 [PMID: 26699542] (WB) WB
Pei C, Qin S, Wang M, Zhang S. Regulatory mechanism of human vascular smooth muscle cell phenotypic transformation induced by NELIN. Mol Med Rep. 2015 Nov [PMID: 26458985] (Human)

Details:
This citation used the FITC version of this antibody.
Human
Tabolacci C, Cordella M, Turcano L et al. Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells Eur. J. Pharmacol. 2015 Jun 03 [PMID: 26048310] (WB, Human) WB Human
Beckmann EA, Kohler AM, Meister C et al. Integration of the catalytic subunit activates deneddylase activity in vivo as final step in fungal COP9 signalosome assembly Mol. Microbiol. 2015 Apr 02 [PMID: 25846252] (WB, Fungi) WB Fungi
Show All 19 Publications.

Reviews for Actin Antibody (NB100-74340) (8) 4.18

Average Rating: 4.1
(Based on 8 reviews)
We have 8 reviews tested in 5 species: Human, Mouse, Drosophila, Mouse and Human, Other.

Reviews using NB100-74340:
Filter by Applications
Simple Western
(1)
WB
(5)
ICC
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Drosophila
(1)
Mouse and Human
(1)
Other
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Caitlin Hair
Simple Western Mouse 03/27/2018
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Summary

ApplicationSimple Western
Sample Tested brain and spinal cord
SpeciesMouse
LotE

Comments

CommentsBaseline ran higher on the samples, however the peaks were consistent across samples.
Western Blot Actin NB100-74340
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reviewed by:
Anonymous
WB Mouse and Human 06/29/2017
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Summary

ApplicationWestern Blot
Sample Tested293ft lysate,NIH 3T3 cell lysate
SpeciesMouse and Human
Lotb-2
Western Blot Actin NB100-74340
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reviewed by:
Alexandra Jette
WB Drosophila 03/21/2017
View

Summary

ApplicationWestern Blot
Sample TestedWhole embryos lysate
SpeciesDrosophila
LotB-2

Comments

CommentsThe antibody was diluted 1/10000 and it worked well.
Western Blot Actin NB100-74340
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reviewed by:
Byron Baron
WB Human 12/24/2016
View

Summary

ApplicationWestern Blot
Sample TestedSW480 (colon)
SpeciesHuman
LotC1

Comments

CommentsPrimary mouse anti Actin antibody diluted 1:1000 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti mouse antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey
Immunocytochemistry Actin NB100-74340
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reviewed by:
Anony- mous
ICC Other 06/11/2014
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Summary

ApplicationImmunocytochemistry
SpeciesOther
Special ApplicationsConfocal immunofluorescent analysis of CHO cells using Actin antibody (NB100-74340, 1:5). An Alexa Fluor 568-conjugated Goat to mouse IgG was used as secondary antibody (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot Actin NB100-74340
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reviewed by:
Anony- mous
WB Human 06/11/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from Jurkat and CHO cells using Actin antibody (NB100-74340, 1:100).
FileView PDF
Immunocytochemistry Actin NB100-74340
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reviewed by:
David Sancho
ICC Other 10/17/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedLeishmania promastigotes
SpeciesOther
CommentsPositive Control: Staining using Novus anti-actin in a macrophage cell line. Negative Control: Isotype control, mouse serum. Staining Expectations: General staining excluding the nucleus of the promastigote. Staining Observation: Di-fuse and sometimes granular staining excluding nucleus of the promastigote (Pink color). DAPI labeling weak in the nucleus and bright in the kinetoplast is used as counterstaining (blue).

Blocking

Blocking DetailsPBS, 5% BSA, 1h, RT overnight at 4C

Primary Anitbody

Dilution RatioPBS, 5% BSA, 1/100, 30 min at 4C

Secondary Antibody

Secondary DescriptionChicken
Secondary Manufacturer Cat#Invitrogen, A21463
Secondary ConcentrationPBS 5% BSA, 1/200, 30min at 4C

Details

Detection NotesZeiss LSM700 Confocal
Fixation DetailsAdherence to Poly-L Lysine treated coverslips, Paraformaldehyde, 4%, 15 min, RT. Triton X-100 0.2% 5 min
Wash DescriptionPBS, EDTA 5mM, Fetal Calf Serum 0.5%. 2 min repetitions twice

Comments

CommentsPositive Control: Staining using Novus anti-actin in a macrophage cell line. Negative Control: Isotype control, mouse serum. Staining Expectations: General staining excluding the nucleus of the promastigote. Staining Observation: Di-fuse and sometimes granular staining excluding nucleus of the promastigote (Pink color). DAPI labeling weak in the nucleus and bright in the kinetoplast is used as counterstaining (blue).
 
reviewed by:
Anonymous
WB Other 11/18/2009
View

Summary

ApplicationWestern Blot
Sample TestedBaking Yeast - Saccharomyces Cerevisiae, Sample Amount: Control Lane - #7 is 1ug
SpeciesOther
Lot793-110

Blocking

Blocking DetailsBlocking Buffer: 3% powdered milk in PBS, Blocking Time: 30 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: PBS + 2% BSA, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti mouse HRP, Secondary Ab Dilution Ratio: 1/2000
Secondary Manufacturer Cat#BIO RAD 1706516

Details

Detection NotesMethod: DAB, Exposure Time: ~1 min, Pos.Ctrl: Purified yeast actin (S. cerevisiae), Neg.Ctrl: No actin, Specific Bands: ~45 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin Antibody (NB100-74340). (Showing 1 - 4 of 4 FAQs).

  1. I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.
  2. One of our customers is interested in one anti-Actin-2, that recognize plant (Arabidopsis and other plants), valid for WB and IP. I have found that Ab at your catalog: Actin Antibody (NB100-74340) - clone: mAbGEa. Please, confirm me if you have available this Ab, and if you have any other that could be valid for this customer.
    • NB100-74340 is an excellent choice for Western blot, but we have not tested it in IP. It is the only antibody that is tested to work in plants, and therefore the only one that I would recommend to your customer.
  3. Do you know whether this antibody reacts with Leishmania actin?
    • NB100-74340, was raised against the entire Arabidopsis actin protein and I did an alignment between Arabidopsis Actin and Leishmania Actin, these two sequences are 71.47 percent identical so I can't guarantee this antibody works for Leishmania but since the homology is high, we can predict cross reactivity. I suggest you look into our Innovator's reward program if you are planning to test this antibody in Leishmania. Learn more about our Innovator's Reward program.
  4. I would like to know the approximate concentration. 
    • The concentration is 1mg/ml.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-74340AF405
Alexa Fluor 488 Labeled NB100-74340AF488
Alexa Fluor 647 Labeled NB100-74340AF647
Alexa Fluor 700 Labeled NB100-74340AF700
DyLight 350 Labeled NB100-74340UV
DyLight 405 Labeled NB100-74340V
DyLight 488 Labeled NB100-74340G
DyLight 680 Labeled NB100-74340FR
DyLight 755 Labeled NB100-74340IR
FITC Labeled NB100-74340F

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Actin NB100-74340

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Blogs on Actin. Showing 1-10 of 16 blog posts - Show all blog posts.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

CRISPR/Cas9: Keep your friends close, but your viruses closer
"CRISPR", or clustered regularly interspaced short palindromic repeats, is an ancient bacterial mechanism that prevents the invasion of foreign pathogens to a host organism.  Specifically, the CRISPR sequence has been identified as a si...  Read full blog post.

Alpha-actin/ACTA1 - A skeletal muscle isoform mutated in various myopathies
Actin is an abundant cytoskeletal protein involved in a variety of cellular processes such as cell motility, cell division, and muscle contraction. Actin monomers assemble into filaments and can provide a track for transport of cargo by the molecul...  Read full blog post.

Alpha-Adducin - Assembling the cytoskeleton meshwork that underlies the plasma membrane
The structure and organization of the plasma membrane is maintained by an underlying network of cytoskeletal proteins including actin and spectrin. Adducin, a member of this protein network, binds to bundles and caps actin filaments and links them...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Actin Dynamics and Endocytic Trafficking
Actin is a ubiquitous and an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes. It is abundant protein whose monomers polymerize into polarized actin filaments, within epithelial cells. Filamentous acti...  Read full blog post.

Beta Actin is a Key Player in Embryonic Development and Cell Motility
Actins are an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes, including cell migration, division, and regulation of gene expression. These functions are attributed to the ability of actin to form fil...  Read full blog post.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Recent Reviews

4.1
5
4
4
2
3
1
2
1
1
0

 
Caitlin Hair
03/27/2018
Application: Simple Western
Species: Mouse

Anonymous
06/29/2017
Application: WB
Species: Mouse and Human

Alexandra Jette
03/21/2017
Application: WB
Species: Drosophila

Bioinformatics

Gene Symbol ACTA1
Entrez