p53 Antibody (184721)

Images

 
Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 μM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 ...read more
p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355) at 3 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP, ICC/IF
Clone
184721
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

p53 Antibody (184721) Summary

Immunogen
E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637
Specificity
Detects human, mouse, and rat p53.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TP53
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 1-2 ug/500 ug cell lysate
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1355 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p53 Antibody (184721)

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p53 Antibody (MAB1355)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB1355 Applications Species
D Li?kiewicz, A Li?kiewicz, M Grabowski, MM Nowacka-Ch, K Jab?o?ska, A Wojakowska, ? Marczak, JJ Barski, A Ma?ecki Upregulation of hepatic autophagy under nutritional ketosis The Journal of nutritional biochemistry, 2021;0(0):108620. 2021 [PMID: 33705944] (Western Blot, Mouse) Western Blot Mouse
J Wang, D Dubiel, Y Wu, Y Cheng, DA Wolf, W Dubiel CSN7B defines a variant COP9 signalosome complex with distinct function in DNA damage response Cell Reports, 2021;34(4):108662. 2021 [PMID: 33503427] (Western Blot, Human) Western Blot Human
Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo Redox Biol, 2016;11(0):219-230. 2016 [PMID: 28012437] (Western Blot, Mouse) Western Blot Mouse
Levy G, Bomze D, Heinz S, Ramachandran S, Noerenberg A, Cohen M, Shibolet O, Sklan E, Braspenning J, Nahmias Y Long-term culture and expansion of primary human hepatocytes. Nat Biotechnol, 2015;33(12):1264-1271. 2015 [PMID: 26501953] (Western Blot, Human) Western Blot Human
Olsson M, Vakifahmetoglu H, Abruzzo PM, Hogstrand K, Grandien A, Zhivotovsky B DISC-mediated activation of caspase-2 in DNA damage-induced apoptosis. Oncogene, 2009;28(18):1949-59. 2009 [PMID: 19347032] (Western Blot, Human) Western Blot Human
Vakifahmetoglu H, Olsson M, Orrenius S, Zhivotovsky B Functional connection between p53 and caspase-2 is essential for apoptosis induced by DNA damage. Oncogene, 2006;25(41):5683-92. 2006 [PMID: 16652156] (Western Blot, Human) Western Blot Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for p53 Antibody (MAB1355)

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Diseases for p53 Antibody (MAB1355)

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Pathways for p53 Antibody (MAB1355)

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PTMs for p53 Antibody (MAB1355)

Learn more about PTMs related to p53 Antibody (MAB1355).

Blogs on p53. Showing 1-10 of 19 blog posts - Show all blog posts.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.

Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels ...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels ...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

Showing 1-10 of 19 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol TP53
Uniprot