IL-1 beta/IL-1F2 Antibody [Unconjugated]

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-)ortreated (+) with 200 nM PMA for 24 hoursand 10 μg/mL LPS for 4 hours andRAW 264.7 mouse monocyte/macrophagecell line untreated (-)or treated (+) with10 μg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Recombinant MouseIL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND50 is typically ≤0.25 µg/mL.

IL‑1 beta /IL‑1F2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

IL‑1 beta /IL‑1F2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-1 beta /IL-1F2
  Val118-Ser269
  Accession # NP_032387
• Specificity: Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant porcine IL‑1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization ≤0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (AF-401-NA)(142)

Publications using AF-401-NA

• J Wang, H Liu, G Xie, W Cai, J Xu Identification of hub genes and key pathways of dietary advanced glycation end products?induced non?alcoholic fatty liver disease by bioinformatics analysis and animal experiments Mol Med Rep, 2020;21(2):685-694. 2020 [PMID: 31974594] (IHC, Mouse)
• J Kim, H Ahn, S Yu, JH Ahn, HJ Ko, MN Kweon, EJ Hong, BS An, E Lee, GS Lee I&amp;kappaB? controls NLRP3 inflammasome activation via upregulation of the Nlrp3 gene Cytokine, 2020;127(0):154983. 2020 [PMID: 31918161] (Western Blot, Mouse)
• A Bhatta, M Atianand, Z Jiang, J Crabtree, J Blin, KA Fitzgerald A Mitochondrial Micropeptide Is Required for Activation of the Nlrp3 Inflammasome J. Immunol., 2019;0(0):. 2019 [PMID: 31836654] (Western Blot, Mouse)
• M Speir, CJ Nowell, AA Chen, JA O'Donnell, IS Shamie, PR Lakin, AA D'Cruz, RO Braun, JJ Babon, RS Lewis, M Bliss-More, I Shlomovitz, S Wang, LH Cengia, AI Stoica, R Hakem, MA Kelliher, LA O'Reilly, H Patsiouras, KE Lawlor, E Weller, NE Lewis, AW Roberts, M Gerlic, BA Croker Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation Nat. Immunol., 2019;21(1):54-64. 2019 [PMID: 31819256] (Western Blot, Mouse)
• T Yoshizaki, S Itoh, S Yamaguchi, T Numata, A Nambu, N Kimura, H Suto, K Okumura, K Sudo, A Yamaguchi, S Nakae IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice Sci Rep, 2019;9(1):17067. 2019 [PMID: 31745167] (IHC, Mouse)
• Y Chen, H Wang, J Shen, R Deng, X Yao, Q Guo, A Lu, B Sun, Y Zhang, G Meng Gasdermin D Drives the Nonexosomal Secretion of Galectin-3, an Insulin Signal Antagonist J. Immunol., 2019;0(0):. 2019 [PMID: 31597705] (Western Blot, Mouse)
• MM Hughes, A Hooftman, S Angiari, P Tummala, Z Zaslona, MC Runtsch, AF McGettrick, CE Sutton, C Diskin, M Rooke, S Takahashi, S Sundararaj, MG Casarotto, JE Dahlstrom, EM Palsson-Mc, SC Corr, KHG Mills, RJS Preston, N Neamati, Y Xie, JB Baell, PG Board, LAJ O'Neill Glutathione Transferase Omega-1 Regulates NLRP3 Inflammasome Activation through NEK7 Deglutathionylation Cell Rep, 2019;29(1):151-161.e5. 2019 [PMID: 31577945] (Western Blot, Mouse)
• P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019;11(18):7402-7415. 2019 [PMID: 31557129] (Neutralization, Mouse)
• P Samir, S Kesavardha, DM Patmore, S Gingras, RKS Malireddi, R Karki, CS Guy, B Briard, DE Place, A Bhattachar, BR Sharma, A Nourse, SV King, A Pitre, AR Burton, S Pelletier, RJ Gilbertson, TD Kanneganti DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome Nature, 2019;0(0):. 2019 [PMID: 31511697] (Western Blot, Mouse)
• MK Akula, MX Ibrahim, EG Ivarsson, OM Khan, IT Kumar, M Erlandsson, C Karlsson, X Xu, M Brisslert, C Brakebusch, D Wang, M Bokarewa, VI Sayin, MO Bergo Protein prenylation restrains innate immunity by inhibiting Rac1 effector interactions Nat Commun, 2019;10(1):3975. 2019 [PMID: 31484924] (Western Blot, Mouse)
• MR Spalinger, TS Schmidt, M Schwarzfis, L Hering, K Atrott, S Lang, C Gottier, A Geirnaert, C Lacroix, X Dai, DJ Rawlings, AC Chan, C von Mering, G Rogler, M Scharl Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner J. Clin. Invest., 2019;130(0):2527-2541. 2019 [PMID: 31107248] (Western Blot, Mouse)
• LS da Costa, A Outlioua, A Anginot, K Akarid, D Arnoult RNA viruses promote activation of the NLRP3 inflammasome through cytopathogenic effect-induced potassium efflux Cell Death Dis, 2019;10(5):346. 2019 [PMID: 31024004] (Western Blot, Mouse)
• DM Ojcius, A Jafari, L Yeruva, CW Schindler, AA Abdul-Sate Dicer regulates activation of the NLRP3 inflammasome PLoS ONE, 2019;14(4):e0215689. 2019 [PMID: 31013297] (Western Blot, Mouse)
• R Meng, L Gu, Y Lu, K Zhao, J Wu, H Wang, J Han, Y Tang, B Lu High mobility group box 1 enables bacterial lipids to trigger receptor-interacting protein kinase 3 (RIPK3)-mediated necroptosis and apoptosis in mice J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31000631] (Western Blot, Mouse)
• H Ahn, BC Han, J Kim, SG Kang, PH Kim, KH Jang, SH So, SH Lee, GS Lee Nonsaponin fraction of Korean Red Ginseng attenuates cytokine production via inhibition of TLR4 expression J Ginseng Res, 2019;43(2):291-299. 2019 [PMID: 30976167] (Western Blot, Mouse)
• P Zhang, L Cao, R Zhou, X Yang, M Wu The lncRNA Neat1 promotes activation of inflammasomes in macrophages Nat Commun, 2019;10(1):1495. 2019 [PMID: 30940803] (Western Blot, Mouse)
• J Lee, E Jung, M Heur Injury induces endothelial to mesenchymal transition in the mouse corneal endothelium in vivo via FGF2 Mol. Vis., 2019;25(0):22-34. 2019 [PMID: 30804659] (Western Blot, Mouse)
• S Morita-Tak, K Nakahara, S Hasegawa-I, A Isonishi, K Tatsumi, H Okuda, T Tanaka, M Kitabatake, T Ito, A Wanaka Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance J Neuroinflammation, 2019;16(1):39. 2019 [PMID: 30764851] (IHC-Fr, Mouse)
• Z Erlich, I Shlomovitz, L Edry-Botze, H Cohen, D Frank, H Wang, AM Lew, KE Lawlor, Y Zhan, JE Vince, M Gerlic Macrophages, rather than DCs, are responsible for inflammasome activity in the GM-CSF BMDC model Nat. Immunol., 2019;0(0):. 2019 [PMID: 30742078] (Western Blot, Mouse)
• WL Hwang, HY Lan, WC Cheng, SC Huang, MH Yang Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer J Hematol Oncol, 2019;12(1):10. 2019 [PMID: 30683126] (Neutralization, Mouse)
• H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury JCI Insight, 2019;0(0):. 2019 [PMID: 30676324] (Western Blot, Mouse)
• RT Gunaratna, A Santos, L Luo, C Nagi, I Lambertz, M Spier, CJ Conti, RS Fuchs-Youn Dynamic role of the codon 72 p53 single-nucleotide polymorphism in mammary tumorigenesis in a humanized mouse model Oncogene, 2019;0(0):. 2019 [PMID: 30651598] (IHC-P, Mouse)
• D Kurundkar, AR Kurundkar, NB Bone, EJ Becker, W Liu, B Chacko, V Darley-Usm, JW Zmijewski, VJ Thannickal SIRT3 diminishes inflammation and mitigates endotoxin-induced acute lung injury JCI Insight, 2019;4(1):. 2019 [PMID: 30626741] (Western Blot, Mouse)
• C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia Sci Rep, 2018;8(1):18062. 2018 [PMID: 30584250] (IHC, Mouse)
• A Mathur, S Feng, JA Hayward, C Ngo, D Fox, II Atmosukart, JD Price, K Schauer, E Märtlbauer, AAB Robertson, G Burgio, EM Fox, SH Leppla, NO Kaakoush, SM Man A multicomponent toxin from Bacillus cereus incites inflammation and shapes host outcome via the NLRP3 inflammasome Nat Microbiol, 2018;0(0):. 2018 [PMID: 30531979] (Western Blot, Mouse)
• JE Vince, D De Nardo, W Gao, AJ Vince, C Hall, K McArthur, D Simpson, S Vijayaraj, LM Lindqvist, P Bouillet, MA Rizzacasa, SM Man, J Silke, SL Masters, G Lessene, DCS Huang, DHD Gray, BT Kile, F Shao, KE Lawlor The Mitochondrial Apoptotic Effectors BAX/BAK Activate Caspase-3 and -7 to Trigger NLRP3 Inflammasome and Caspase-8 Driven IL-1? Activation Cell Rep, 2018;25(9):2339-2353.e4. 2018 [PMID: 30485804] (Western Blot, Mouse)
• B Koch, AA Barugahare, TL Lo, C Huang, RB Schittenhe, DR Powell, TH Beilharz, A Traven A Metabolic Checkpoint for the Yeast-to-Hyphae Developmental Switch Regulated by Endogenous Nitric Oxide Signaling Cell Rep, 2018;25(8):2244-2258.e7. 2018 [PMID: 30463019] (Western Blot, Mouse)
• D Sharma, A Malik, C Guy, P Vogel, TD Kanneganti TNF/TNFR axis promotes pyrin inflammasome activation and distinctly modulates pyrin inflammasomopathy J. Clin. Invest., 2018;0(0):. 2018 [PMID: 30457980] (Western Blot, Mouse)
• L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes Nat Commun, 2018;9(1):4260. 2018 [PMID: 30323213]
• S Wang, Y Lin, X Yuan, F Li, L Guo, B Wu REV-ERB? integrates colon clock with experimental colitis through regulation of NF-?B/NLRP3 axis Nat Commun, 2018;9(1):4246. 2018 [PMID: 30315268] (Western Blot, Mouse)
• JT Benjamin, DJ Moore, C Bennett, R van der Me, A Royce, R Loveland, JL Wynn Cutting Edge: IL-1? and Not IL-1? Drives IL-1R1-Dependent Neonatal Murine Sepsis Lethality J. Immunol., 2018;0(0):. 2018 [PMID: 30305325] (Western Blot, Mouse)
• Z Zhang, X Shao, N Jiang, S Mou, L Gu, S Li, Q Lin, Y He, M Zhang, W Zhou, Z Ni Caspase-11-mediated tubular epithelial pyroptosis underlies contrast-induced acute kidney injury Cell Death Dis, 2018;9(10):983. 2018 [PMID: 30250284] (IHC-P, Mouse)
• Y Chen, X He, X Yuan, J Hong, O Bhat, G Li, PL Li, J Guo NLRP3 Inflammasome Formation and Activation in Nonalcoholic Steatohepatitis: Therapeutic Target for Antimetabolic Syndrome Remedy FTZ Oxid Med Cell Longev, 2018;2018(0):2901871. 2018 [PMID: 30140364] (IHC, Mouse)
• M Monteleone, AC Stanley, KW Chen, DL Brown, JS Bezbradica, JB von Pein, CL Holley, D Boucher, MR Shakespear, R Kapetanovi, V Rolfes, MJ Sweet, JL Stow, K Schroder Interleukin-1? Maturation Triggers Its Relocation to the Plasma Membrane for Gasdermin-D-Dependent and -Independent Secretion Cell Rep, 2018;24(6):1425-1433. 2018 [PMID: 30089254] (ICC, Mouse)
• C Marchetti, B Swartzwelt, MI Koenders, T Azam, IW Tengesdal, N Powers, DM de Graaf, CA Dinarello, LAB Joosten NLRP3 inflammasome inhibitor OLT1177 suppresses joint inflammation in murine models of acute arthritis Arthritis Res. Ther., 2018;20(1):169. 2018 [PMID: 30075804] (Western Blot, Mouse)
• M Shahid, MY Lee, A Yeon, E Cho, V Sairam, L Valdiviez, S You, J Kim Menthol, a unique urinary volatile compound, is associated with chronic inflammation in interstitial cystitis Sci Rep, 2018;8(1):10859. 2018 [PMID: 30022124] (Western Blot, Mouse)
• H He, H Jiang, Y Chen, J Ye, A Wang, C Wang, Q Liu, G Liang, X Deng, W Jiang, R Zhou Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity Nat Commun, 2018;9(1):2550. 2018 [PMID: 29959312] (Western Blot, Mouse)
• E Lee, I Hwang, S Park, S Hong, B Hwang, Y Cho, J Son, JW Yu MPTP-driven NLRP3 inflammasome activation in microglia plays a central role in dopaminergic neurodegeneration Cell Death Differ., 2018;0(0):. 2018 [PMID: 29786072] (Western Blot, Mouse)
• SB Kwak, S Koppula, EJ In, X Sun, YK Kim, MK Kim, KH Lee, TB Kang Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation Mediators Inflamm., 2018;2018(0):6054069. 2018 [PMID: 29686531] (Western Blot, Mouse)
• LG Coleman, R Maile, SW Jones, BA Cairns, FT Crews HMGB1/IL-1? complexes in plasma microvesicles modulate immune responses to burn injury PLoS ONE, 2018;13(3):e0195335. 2018 [PMID: 29601597] (Western Blot, Mouse)
• YK Kim, S Koppula, DW Shim, EJ In, SB Kwak, MK Kim, SH Yu, KH Lee, TB Kang Inhibitory Effect and Mechanism ofArctium lappaExtracton NLRP3Inflammasome Activation Evid Based Complement Alternat Med, 2018;2018(0):6346734. 2018 [PMID: 29576797] (Western Blot, Mouse)
• C Cochain, E Vafadarnej, P Arampatzi, P Jaroslav, H Winkels, K Ley, D Wolf, AE Saliba, A Zernecke Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis Circ. Res., 2018;0(0):. 2018 [PMID: 29545365] (IHC, Mouse)
• Y Huang, H Jiang, Y Chen, X Wang, Y Yang, J Tao, X Deng, G Liang, H Zhang, W Jiang, R Zhou Tranilast directly targets NLRP3 to treat inflammasome-driven diseases EMBO Mol Med, 2018;0(0):. 2018 [PMID: 29531021] (Western Blot, Mouse)
• LH Chu, M Indramohan, RA Ratsimandr, A Gangopadhy, EP Morris, DM Monack, A Dorfleutne, C Stehlik The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages Nat Commun, 2018;9(1):996. 2018 [PMID: 29520027] (Western Blot, Mouse)
• W Zhuang, C Wang, X Shi, S Qiu, S Zhang, B Xu, M Chen, W Jiang, H Dong, Y Qiao MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss Int. J. Mol. Med., 2018;0(0):. 2018 [PMID: 29512778] (Western Blot, Mouse)
• RG Carroll, Z Zas?ona, S Galván-Peñ, EL Koppe, DC Sévin, S Angiari, M Triantafil, K Triantafil, LK Modis, LA O'Neill An unexpected link between fatty acid synthase and cholesterol synthesis in proinflammatory macrophage activation J. Biol. Chem., 2018;293(15):5509-5521. 2018 [PMID: 29463677] (Western Blot, Mouse)
• JC Santos, MS Dick, B Lagrange, D Degrandi, K Pfeffer, M Yamamoto, E Meunier, P Pelczar, T Henry, P Broz LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation EMBO J., 2018;0(0):. 2018 [PMID: 29459437] (Western Blot, Mouse)
• D Boucher, M Monteleone, RC Coll, KW Chen, CM Ross, JL Teo, GA Gomez, CL Holley, D Bierschenk, KJ Stacey, AS Yap, JS Bezbradica, K Schroder Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity J. Exp. Med., 2018;0(0):. 2018 [PMID: 29432122] (Western Blot, Mouse)
• JL Ross, LF Queme, JE Lamb, KJ Green, MP Jankowski Sex differences in primary muscle afferent sensitization following ischemia and reperfusion injury Biol Sex Differ, 2018;9(1):2. 2018 [PMID: 29298725] (Western Blot, Mouse)
• K Zenke, M Muroi, KI Tanamoto AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner Cell. Signal., 2017;43(0):32-39. 2017 [PMID: 29242168] (Western Blot, Mouse)
• S Ghosh, C Wallerath, S Covarrubia, V Hornung, S Carpenter, KA Fitzgerald The PYHIN Protein p205 Regulates the Inflammasome by Controlling Asc Expression J. Immunol., 2017;0(0):. 2017 [PMID: 28931603] (Western Blot, Mouse)
• JC Rivera, B Noueihed, A Madaan, I Lahaie, J Pan, J Belik, S Chemtob Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina J Neuroinflammation, 2017;14(1):181. 2017 [PMID: 28874201] (IHC, Mouse)
• F Albalawi, W Lu, JM Beckel, JC Lim, SA McCaughey, CH Mitchell The P2X7 Receptor Primes IL-1? and the NLRP3 Inflammasome in Astrocytes Exposed to Mechanical Strain Front Cell Neurosci, 2017;11(0):227. 2017 [PMID: 28848393] (Western Blot, Mouse)
• EM McDonough, CW Barrett, B Parang, MK Mittal, JJ Smith, AM Bradley, YA Choksi, LA Coburn, SP Short, JJ Thompson, B Zhang, SV Poindexter, MA Fischer, X Chen, J Li, FL Revetta, R Naik, MK Washington, MJ Rosen, SW Hiebert, KT Wilson, CS Williams MTG16 is a tumor suppressor in colitis-associated carcinoma JCI Insight, 2017;2(16):. 2017 [PMID: 28814670] (IHC, Mouse)
• B Brawek, Y Liang, D Savitska, K Li, N Fomin-Thun, Y Kovalchuk, E Zirdum, J Jakobsson, O Garaschuk A new approach for ratiometric in vivo calcium imaging of microglia Sci Rep, 2017;7(1):6030. 2017 [PMID: 28729628]
• S Yoon, A Kovalenko, K Bogdanov, D Wallach MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation Immunity, 2017;0(0):. 2017 [PMID: 28666573] (Western Blot, Mouse)
• T Wu, J Zhang, M Geng, SJ Tang, W Zhang, J Shu Nucleoside reverse transcriptase inhibitors (NRTIs) induce proinflammatory cytokines in the CNS via Wnt5a signaling Sci Rep, 2017;7(1):4117. 2017 [PMID: 28646196] (Western Blot, Mouse)
• R Domingo-Fe, RC Coll, J Kearney, S Breit, LA O'Neill The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1? transcription and activate the NLRP3 inflammasome J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28576828] (Western Blot, Mouse)
• V Neudecker, M Haneklaus, O Jensen, L Khailova, JC Masterson, H Tye, K Biette, P Jedlicka, KS Brodsky, ME Gerich, M Mack, AAB Robertson, MA Cooper, GT Furuta, CA Dinarello, LA O'Neill, HK Eltzschig, SL Masters, EN McNamee Myeloid-derived miR-223 regulates intestinal inflammation via repression of the NLRP3 inflammasome J. Exp. Med., 2017;0(0):. 2017 [PMID: 28487310] (Western Blot, Mouse)
• Q Zhu, TD Kanneganti Cutting Edge: Distinct Regulatory Mechanisms Control Proinflammatory Cytokines IL-18 and IL-1? J. Immunol., 2017;0(0):. 2017 [PMID: 28468974] (Western Blot, Mouse)
• H Ahn, E Jeon, JC Kim, SG Kang, SI Yoon, HJ Ko, PH Kim, GS Lee Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production Sci Rep, 2017;7(1):1314. 2017 [PMID: 28465544] (Western Blot, Mouse)
• Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28438835] (IHC, Mouse)
• L Freeman, H Guo, CN David, WJ Brickey, S Jha, JP Ting NLR members NLRC4 and NLRP3 mediate sterile inflammasome activation in microglia and astrocytes J. Exp. Med., 2017;0(0):. 2017 [PMID: 28404595] (Western Blot, Mouse)
• MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects J. Exp. Med., 2017;214(5):1297-1311. 2017 [PMID: 28400473] (Western Blot, Mouse)
• Z Zhong, Y Zhai, P Bu, S Shah, L Qiao Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc(Min/+) mice Nat Commun, 2017;8(0):15004. 2017 [PMID: 28397782] (Western Blot, Mouse)
• H Youssef, P Stashenko Interleukin-1 and estrogen protect against disseminating dentoalveolar infections Int J Oral Sci, 2017;9(1):16-23. 2017 [PMID: 28358036] (Neutralization, Mouse)
• K Moriwaki, S Balaji, J Bertin, PJ Gough, FK Chan Distinct Kinase-Independent Role of RIPK3 in CD11c(+) Mononuclear Phagocytes in Cytokine-Induced Tissue Repair Cell Rep, 2017;18(10):2441-2451. 2017 [PMID: 28273458] (Western Blot, Mouse)
• TP Domiciano, D Wakita, HD Jones, TR Crother, WA Verri, M Arditi, K Shimada Quercetin Inhibits Inflammasome Activation by Interfering with ASC Oligomerization and Prevents Interleukin-1 Mediated Mouse Vasculitis Sci Rep, 2017;7(0):41539. 2017 [PMID: 28148962] (Western Blot, Mouse)
• M Tsugita, N Morimoto, M Tashiro, K Kinoshita, M Nakayama SR-B1 Is a Silica Receptor that Mediates Canonical Inflammasome Activation Cell Rep, 2017;18(5):1298-1311. 2017 [PMID: 28147282] (Western Blot, Mouse)
• O Tufanli, P Telkoparan, D Acosta-Alv, B Kocaturk, UI Onat, SM Hamid, I €imen, P Walter, C Weber, E Erbay Targeting IRE1 with small molecules counteracts progression of atherosclerosis Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28137856] (Western Blot, Mouse)
• HJ Koh, YR Kim, JS Kim, JS Yun, K Jang, CS Yang Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKC� PLoS Pathog, 2017;13(1):e1006126. 2017 [PMID: 28125719] (Western Blot, Mouse)
• Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis Sci Rep, 2016;6(0):39257. 2016 [PMID: 27995963] (Western Blot, Mouse)
• Functional and Evolutionary Analyses Identify Proteolysis as a General Mechanism for NLRP1 Inflammasome Activation PLoS Pathog, 2016;12(12):e1006052. 2016 [PMID: 27926929] (Western Blot, Human)
• TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158. 2016 [PMID: 27911817] (Western Blot, Mouse)
• Jeremy S Duffield Pericyte MyD88 and IRAK4 control inflammatory and fibrotic responses to tissue injury J. Clin. Invest., 2016;0(0):. 2016 [PMID: 27869651] (Western Blot, Mouse)
• CARD9 negatively regulates NLRP3-induced IL-1? production on Salmonella infection of macrophages Nat Commun, 2016;7(0):12874. 2016 [PMID: 27670879] (Western Blot, Mouse)
• Lymphatic vessels regulate immune microenvironments in human and murine melanoma J Clin Invest, 2016;0(0):. 2016 [PMID: 27525437] (Western Blot, Human)
• CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27515950] (Neutralization, Mouse)
• C13orf31 (FAMIN) is a central regulator of immunometabolic function Nat Immunol, 2016;0(0):. 2016 [PMID: 27478939] (Western Blot, Mouse)
• HIF-1? Is an Essential Mediator of IFN-?-Dependent Immunity to Mycobacterium tuberculosis J Immunol, 2016;0(0):. 2016 [PMID: 27430718] (Western Blot, Mouse)
• Enzymatically inactive procaspase-1 stabilizes the ASC-pyroptosome and supports pyroptosome spreading during cell division J Biol Chem, 2016;0(0):. 2016 [PMID: 27402835] (Bead-based Immunoassay, Mouse)
• Activation of NLRP3 inflammasomes in mouse hepatic stellate cells during Schistosoma J. infection Oncotarget, 2016;7(26):39316-39331. 2016 [PMID: 27322427] (IHC, Mouse)
• Yong Hwan Park Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS Nat Immunol, 2016;0(0):. 2016 [PMID: 27270401] (Western Blot, Mouse)
• Gang Hu Fluoxetine Inhibits NLRP3 Inflammasome Activation: Implication in Depression Int. J. Neuropsychopharmacol., 2016;19(9):. 2016 [PMID: 27207922] (Western Blot, Mouse)
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• Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice. J Immunol, 2015;195(3):1233-41. 2015 [PMID: 26116505] (Western Blot, Mouse)
• Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules. J Immunol, 2015;194(8):3656-63. 2015 [PMID: 25795756]
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• Chien C, Lee M, Liou H, Liou H, Fu W Local immunosuppressive microenvironment enhances migration of melanoma cells to lungs in DJ-1 knockout mice. PLoS ONE, 2015;10(2):e0115827. 2015 [PMID: 25706411] (Neutralization, Mouse)
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• Li B, Gurung P, Malireddi R, Vogel P, Kanneganti T, Geiger T IL-10 engages macrophages to shift Th17 cytokine dependency and pathogenicity during T-cell-mediated colitis. Nat Commun, 2015;6(0):6131. 2015 [PMID: 25607885] (Western Blot, Mouse)
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• Lim, Yu-Mi, Lim, Hyejin, Hur, Kyu Yeon, Quan, Wenying, Lee, Hae-Youn, Cheon, Hwanju, Ryu, Dongryeo, Koo, Seung-Ho, Kim, Hong Lim, Kim, Jin, Komatsu, Masaaki, Lee, Myung-Sh Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes. Nat Commun, 2014;5(0):4934. 2014 [PMID: 25255859] (Western Blot, Mouse)
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• Asrat S, Dugan A, Isberg R The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PLoS Pathog, 2014;10(7):e1004229. 2014 [PMID: 25058342] (Western Blot, Mouse)
• Kajiwara Y, Schiff T, Voloudakis G, Gama Sosa M, Elder G, Bozdagi O, Buxbaum J A critical role for human caspase-4 in endotoxin sensitivity. J Immunol, 2014;193(1):335-43. 2014 [PMID: 24879791] (Western Blot, Mouse)
• Zhao C, Gillette D, Li X, Zhang Z, Wen H Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2 inflammasome activation. J Biol Chem, 2014;289(24):17020-9. 2014 [PMID: 24798340] (Western Blot, Mouse)
• Nieto-Torres J, DeDiego M, Verdia-Baguena C, Jimenez-Guardeno J, Regla-Nava J, Fernandez-Delgado R, Castano-Rodriguez C, Alcaraz A, Torres J, Aguilella V, Enjuanes L Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis. PLoS Pathog, 2014;10(5):e1004077. 2014 [PMID: 24788150] (Western Blot, Mouse)
• Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature, 2014;509(7500):366-70. 2014 [PMID: 24739961] (Western Blot, Mouse)
• Rabolli V, Wallemme L, Lo Re S, Uwambayinema F, Palmai-Pallag M, Thomassen L, Tyteca D, Octave J, Marbaix E, Lison D, Devuyst O, Huaux F Critical role of aquaporins in interleukin 1beta (IL-1beta)-induced inflammation. J Biol Chem, 2014;289(20):13937-47. 2014 [PMID: 24700466] (Western Blot, Mouse)
• Gupta R, Ghosh S, Monks B, DeOliveira R, Tzeng T, Kalantari P, Nandy A, Bhattacharjee B, Chan J, Ferreira F, Rathinam V, Sharma S, Lien E, Silverman N, Fitzgerald K, Firon A, Trieu-Cuot P, Henneke P, Golenbock D RNA and beta-hemolysin of group B Streptococcus induce interleukin-1beta (IL-1beta) by activating NLRP3 inflammasomes in mouse macrophages. J Biol Chem, 2014;289(20):13701-5. 2014 [PMID: 24692555] (Western Blot, Mouse)
• Xia M, Boini K, Abais J, Xu M, Zhang Y, Li P Endothelial NLRP3 inflammasome activation and enhanced neointima formation in mice by adipokine visfatin. Am J Pathol, 2014;184(5):1617-28. 2014 [PMID: 24631027] (IHC, Mouse)
• Kanarek N, Grivennikov S, Leshets M, Lasry A, Alkalay I, Horwitz E, Shaul Y, Stachler M, Voronov E, Apte R, Pagano M, Pikarsky E, Karin M, Ghosh S, Ben-Neriah Y Critical role for IL-1beta in DNA damage-induced mucositis. Proc Natl Acad Sci U S A, 2014;111(6):E702-11. 2014 [PMID: 24469832] (IHC, Mouse)
• Maier N, Crown D, Liu J, Leppla S, Moayeri M Arsenic trioxide and other arsenical compounds inhibit the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes. J Immunol, 2014;192(2):763-70. 2014 [PMID: 24337744] (Western Blot, Human)
• Tanaka S, Ishii A, Ohtaki H, Shioda S, Yoshida T, Numazawa S Activation of microglia induces symptoms of Parkinson&#039;s disease in wild-type, but not in IL-1 knockout mice. J Neuroinflammation, 2013;10(0):143. 2013 [PMID: 24289537] (IHC-Fr, Mouse)
• He Y, Varadarajan S, Munoz-Planillo R, Burberry A, Nakamura Y, Nunez G 3,4-methylenedioxy-beta-nitrostyrene inhibits NLRP3 inflammasome activation by blocking assembly of the inflammasome. J Biol Chem, 2014;289(2):1142-50. 2014 [PMID: 24265316] (Western Blot, Mouse)
• Smedberg J, Westcott M, Ahmed M, Lyles D Signaling pathways in murine dendritic cells that regulate the response to vesicular stomatitis virus vectors that express flagellin. J Virol, 2014;88(2):777-85. 2014 [PMID: 24198430] (Western Blot, Mouse)
• Li B, Shi Y, Shu J, Gao J, Wu P, Tang S Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways. J Biol Chem, 2013;288(19):13610-9. 2013 [PMID: 23539626] (Western Blot, Mouse)
• El-Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, Eaton K, Merchant J Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets. PLoS ONE, 2013;8(3):e58935. 2013 [PMID: 23520544] (IHC, Mouse)
• Misawa T, Takahama M, Kozaki T, Lee H, Zou J, Saitoh T, Akira S Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome. Nat Immunol, 2013;14(5):454-60. 2013 [PMID: 23502856] (Western Blot, Mouse)
• Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E The role of IL-1beta in the early tumor cell-induced angiogenic response. J Immunol, 2013;190(7):3500-9. 2013 [PMID: 23475218] (Neutralization, Mouse)
• Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature, 2012;492(7427):123-7. 2012 [PMID: 23143333] (Western Blot, Mouse)
• Broz P, Ruby T, Belhocine K, Bouley D, Kayagaki N, Dixit V, Monack D Caspase-11 increases susceptibility to Salmonella infection in the absence of caspase-1. Nature, 2012;490(7419):288-91. 2012 [PMID: 22895188] (Western Blot, Mouse)
• Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus. Infect. Immun., 2012;80(1):410-7. 2012 [PMID: 22038916] (Neutralization, Mouse)
• Kim D, You B, Lim H, Lee SJ Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury. Mol Pain, 2011;7(0):74. 2011 [PMID: 21951975] (ICC, Mouse)
• Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. J. Immunol., 2011;187(9):4835-43. 2011 [PMID: 21930960] (Neutralization, Mouse)
• Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. J. Clin. Invest., 2011;121(10):3846-59. 2011 [PMID: 21926464] (Neutralization, Mouse)
• Bergsbaken T, Fink SL, den Hartigh AB, Loomis WP, Cookson BT Coordinated Host Responses during Pyroptosis: Caspase-1-Dependent Lysosome Exocytosis and Inflammatory Cytokine Maturation. J. Immunol., 2011;187(5):2748-54. 2011 [PMID: 21804020] (Western Blot, Mouse)
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• Yoshida H, Russell J, Senchenkova EY, Almeida Paula LD, Granger DN Interleukin-1beta mediates the extra-intestinal thrombosis associated with experimental colitis. Am. J. Pathol., 2010;177(6):2774-81. 2010 [PMID: 20971730] (Neutralization, Mouse)
• Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes. Nat. Immunol., 2010;11(10):897-904. 2010 [PMID: 20835230] (Western Blot, Mouse)
• McCoy AJ, Koizumi Y, Toma C, Higa N, Dixit V, Taniguchi S, Tschopp J, Suzuki T Cytotoxins of the human pathogen Aeromonas hydrophila trigger, via the NLRP3 inflammasome, caspase-1 activation in macrophages. Eur. J. Immunol., 2010;40(10):2797-803. 2010 [PMID: 20722078] (Western Blot, Mouse)
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Reviews for IL-1 beta/IL-1F2 Antibody (AF-401-NA) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23160153
• Species: Mouse

Blocking

• Blocking Details: See PMID 23160153

Details

• Detection Notes: See PMID 23160153

Comments

• Comments: Published: See PMID 23160153Independent review from antibodyresource.comMouse IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab, Goat IgG

reviewed by: Anonymous ICC Mouse 01/08/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23920123
• Species: Mouse

Blocking

• Blocking Details: See PMID 23920123

Details

• Detection Notes: See PMID 23920123

Comments

• Comments: Published: See PMID 23920123Independent review from antibodyresource.com

reviewed by: Anonymous ELISA Mouse 01/08/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 21709053
• Species: Mouse

Blocking

• Blocking Details: See PMID 21709053

Details

• Detection Notes: See PMID 21709053

Comments

• Comments: Published: See PMID 21709053Independent review from antibodyresource.com

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • NP_032387()