IL-1 beta/IL-1F2 Antibody (615417) [Unconjugated] - Propeptide

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 beta /IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Mouse Anti-Human IL-1 beta /IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 Propeptide at approximately 40 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clone: 615417
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody (615417) [Unconjugated] - Propeptide Summary

• Immunogen: E. coli-derived recombinant human IL-1 beta /IL-1F2 Propeptide
  Met1-Asp116
  Accession # P01584
• Specificity: Detects human IL-1 beta /IL-1F2 Propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1F7, rhIL-1F10, rhIL-18, rhIL-36 alpha, rhIL-36 beta, IL-36 gamma, rhIL-36Ra, recombinant mouse IL-1Ra, or the mature peptides of recombinant IL-1 beta from canine, cotton rat, equine, feline, guinea pig, human, mouse, porcine, rabbit, rat, or rhesus is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (615417) [Unconjugated] - Propeptide

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 beta , also known as IL1F2, is a prototypical member of the IL-1 superfamily. It is produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. The biological activity of IL-1 beta is mediated by the heterodimeric receptor complex containing IL-1 R1 (IL-1 RI) and IL-1 R3 (IL-1 RAcP). The IL-1 beta propeptide is cleaved intracellularly by caspase-1/ICE to generate a 17 kDa active cytokine. The propeptide of human IL-1 beta shares approximately 55% aa sequence identity with the propeptides of mouse and rat IL-1 beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB6964)(8)

Publications using MAB6964

• de Jong, MME;Fokkema, C;Papazian, N;Czeti, Á;Appelman, MK;Vermeulen, M;van Heusden, T;Hoogenboezem, RM;van Beek, G;Tahri, S;Sanders, MA;van de Woestijne, PC;Gay, F;Moreau, P;Büttner-Herold, M;Bruns, H;van Duin, M;Broijl, A;Sonneveld, P;Cupedo, T; An IL-1?-driven neutrophil-stromal cell axis fosters a BAFF-rich protumor microenvironment in individuals with multiple myeloma Nature immunology 2024-04-10 [PMID: 38600356] (Immunohistochemistry, Human)
• H Guo, L Sun, S Ling, JW Xu Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats Mediators Inflamm., 2018-08-12;2018(0):7304096. 2018-08-12 [PMID: 30158835] (Western Blot, Human)
• EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation Respir. Res., 2017-06-28;18(1):131. 2017-06-28 [PMID: 28659178] (Western Blot, Human)
• Y Wang, Z Han, Y Fan, J Zhang, K Chen, L Gao, H Zeng, J Cao, C Wang MicroRNA-9 Inhibits NLRP3 Inflammasome Activation in Human Atherosclerosis Inflammation Cell Models through the JAK1/STAT Signaling Pathway Cell. Physiol. Biochem, 2017-03-27;41(4):1555-1571. 2017-03-27 [PMID: 28334721] (Western Blot, Human)
• NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis Arch Med Sci, 2016-07-20;12(5):950-958. 2016-07-20 [PMID: 27695484] (Western Blot, Human)
• Lili Wang, Peng Qu, Jian Zhao, Yeting Chang NLRP3 and downstream cytokine expression elevated in the monocytes of patients with coronary artery disease Archives of Medical Science 2014-08-29 [PMID: 25276166]
• L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages. J Lipid Res, 2013-09-03;54(11):2998-3008. 2013-09-03 [PMID: 24006511] (Western Blot, Human)
• Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes. J Invest Dermatol, 2013-07-24;134(2):381-8. 2013-07-24 [PMID: 23884315] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P01584()