Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Images

 
Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This ...read more
Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP (Horseradish Peroxidase)

Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Simple Western
  • Western Blot
Publications
Read Publications using
HAF109 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF109)(39)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Virus.

We have publications tested in 8 applications: Bioassay, ELISA Detection, ELISA Development, Flow Cytometry, IHC-Fr, Secondary Antibody, Western Blot, Western Blots.


Filter By Application
Bioassay
(1)
ELISA Detection
(1)
ELISA Development
(2)
Flow Cytometry
(1)
IHC-Fr
(1)
Secondary Antibody
(1)
Western Blot
(28)
Western Blots
(1)
All Applications
Filter By Species
Human
(20)
Mouse
(13)
Rat
(2)
Canine
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using HAF109 Applications Species
P Szodoray, TK Andersen, J Heinzelbec, JF Imbery, PC Huszthy, SM Stanford, B Bogen, OB Landsverk, N Bottini, A Tveita, LA Munthe, B Nakken Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity Cell Reports, 2021;36(6):109525. 2021 [PMID: 34380042]
A Chrysantho, E Gkaliagkou, A Lazaridis, S Arelaki, P Pateinakis, M Ntinopoulo, A Mitsios, C Antoniadou, C Argyriou, GS Georgiadis, V Papadopoul, A Giatromano, K Ritis, P Skendros Angiotensin II triggers neutrophil extracellular traps release linking thromboinflammation with essential hypertension JCI Insight, 2021;0(0):. 2021 [PMID: 34324440] (ELISA Detection, Human) ELISA Detection Human
A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus Nature Communications, 2021;12(1):3739. 2021 [PMID: 34145258] (Western Blot, Mouse) Western Blot Mouse
J Frew, HB Nygaard Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021;9(1):57. 2021 [PMID: 33795008] (Western Blot, Mouse) Western Blot Mouse
J Baggen, L Persoons, E Vanstreels, S Jansen, D Van Loover, B Boeckx, V Geudens, J De Man, D Jochmans, J Wauters, E Wauters, BM Vanaudenae, D Lambrechts, J Neyts, K Dallmeier, HJ Thibaut, M Jacquemyn, P Maes, D Daelemans Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2 Nature Genetics, 2021;0(0):. 2021 [PMID: 33686287] (Western Blot, Human) Western Blot Human
N Burrows, RJM Bashford-R, VJ Bhute, A Peñalver, JR Ferdinand, BJ Stewart, JEG Smith, M Deobagkar-, G Giudice, TM Connor, A Inaba, L Bergamasch, S Smith, MGB Tran, E Petsalaki, PA Lyons, M Espeli, BJP Huntly, KGC Smith, RJ Cornall, MR Clatworthy, PH Maxwell Dynamic regulation of hypoxia-inducible factor-1&alpha activity is essential for normal B cell development Nat. Immunol., 2020;0(0):. 2020 [PMID: 32868930] (Mouse) Mouse
A Göbel, VM Zinna, S Dell'Endic, N Jaschke, JD Kuhlmann, P Wimberger, TD Rachner Anti-tumor effects of mevalonate pathway inhibition in ovarian cancer BMC Cancer, 2020;20(1):703. 2020 [PMID: 32727400] (Western Blot, Human) Western Blot Human
Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice Mol. Ther., 2020;0(0):. 2020 [PMID: 32652045] (Western Blot, Human) Western Blot Human
RM Gorczynski, T Maqbool, G Hoffmann Combined IMIG and Immune Ig Attenuate Allergic Responses in Beagle Dogs J Immunol Res, 2020;2020(0):2061609. 2020 [PMID: 32377529] (ELISA Development, Canine) ELISA Development Canine
K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions J Neuroinflammation, 2020;17(1):141. 2020 [PMID: 32359362] (Western Blot, Rat) Western Blot Rat
Show All 39 Publications.

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Product General Protocols

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Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1