Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Images

 
Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This ...read more
Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP (Horseradish Peroxidase)

Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1

Applications/Dilutions

Publications
Read Publications using
HAF109 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (HAF109)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Bioassay, ELISA Development, IHC Frozen, WB.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
IHC Frozen
(1)
WB
(16)
All Applications
Filter By Species
Human
(11)
Mouse
(7)
Rat
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using HAF109 Applications Species
MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity Nat Commun, 2017;8(1):552. 2017 [PMID: 28916756] (WB,, Human) WB Human
Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix Stem Cell Res Ther, 2017;8(1):189. 2017 [PMID: 28807034] (WB,, Mouse) WB Mouse
IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells Int J Mol Sci, 2017;18(1):. 2017 [PMID: 28117756] (WB,, Human) WB Human
Filomena Gabriella Fulcoli Rebalancing gene haploinsufficiency in vivo by targeting chromatin Nat Commun, 2016;7(0):11688. 2016 [PMID: 27256596] (WB,, Mouse) WB Mouse
Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation. Sci Rep, 2015;5(0):9675. 2015 [PMID: 25872908] (Bioassay,, Human) Bioassay Human
Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland. PLoS ONE, 2014;9(7):e103230. 2014 [PMID: 25054204] (IHC Frozen,, Human) IHC Frozen Human
Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole. PLoS ONE, 2014;9(5):e97419. 2014 [PMID: 24846271] (WB,, Rat) WB Rat
Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Lab Invest, 2014;94(7):752-65. 2014 [PMID: 24791743] (WB,, Mouse) WB Mouse
Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort. PLoS ONE, 2014;9(1):e85436. 2014 [PMID: 24416410] (WB,, Human) WB Human
Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum. J Immunol, 2013;190(7):3427-37. 2013 [PMID: 23455499] (WB,, Mouse) WB Mouse
Show All 19 Publications.

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Bioinformatics Tool for IgG Antibody (HAF109)

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Blogs on IgG.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1