Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]


Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment more
Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This more

Product Details

Reactivity GtSpecies Glossary
Applications WB, Simple Western
HRP (Horseradish Peroxidase)

Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Goat IgG
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Purity Statement
Antigen Affinity-purified


  • Simple Western
  • Western Blot
Read Publications using
HAF109 in the following applications:

Packaging, Storage & Formulations

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
No Preservative


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG


R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF109)(50)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bacteria, Bovine, Canine, N/A, Virus.

We have publications tested in 10 applications: Bioassay, Co-Immunoprecipitation, ELISA Detection, ELISA Development, Flow Cytometry, IHC, IHC-Fr, Secondary Antibody, Western Blot, Western Blots.

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Secondary Antibody
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Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using HAF109 Applications Species
Shrestha, M;Wang, DY;Ben-David, Y;Zacksenhaus, E; CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer Oncogenesis 2023-05-26 [PMID: 37230983] (Western Blot, Mouse) Western Blot Mouse
N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice Frontiers in Physiology, 2023-01-04;13(0):1061715. 2023-01-04 [PMID: 36685192] (Western Blot, Mouse, N/A) Western Blot Mouse, N/A
G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells International Journal of Molecular Sciences, 2022-10-22;23(21):. 2022-10-22 [PMID: 36361539] (Western Blot, Human) Western Blot Human
NC Hait, A Maiti, R Wu, VL Andersen, CC Hsu, Y Wu, DG Chapla, K Takabe, ME Rusiniak, W Bshara, J Zhang, KW Moremen, JTY Lau Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness Cancer Gene Therapy, 2022-06-08;0(0):. 2022-06-08 [PMID: 35676533] (Western Blot, Human) Western Blot Human
D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice Cellular and Molecular Gastroenterology and Hepatology, 2022-06-02;0(0):. 2022-06-02 [PMID: 35660025] (Western Blot, Mouse) Western Blot Mouse
HL Deissler, M Rehak, A Wolf Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A165 No Longer Depends on the Growth Factor's Presence Biomolecules, 2022-05-23;12(5):. 2022-05-23 [PMID: 35625661] (Western Blot, Bovine) Western Blot Bovine
J Sidor, M Gillette, LA Dezi, G Untiveros, L Strizzi Role of Presenilin-1 in Aggressive Human Melanoma International Journal of Molecular Sciences, 2022-04-28;23(9):. 2022-04-28 [PMID: 35563300] (Western Blot, Human) Western Blot Human
Z Liu, Y Li, Y Wang, Z Liu, Y Su, Q Ma, R Han Lipoprotein(a), an Opsonin, Enhances the Phagocytosis of Nontypeable Haemophilus influenzae by Macrophages Journal of Immunology Research, 2021-11-02;2021(0):2185568. 2021-11-02 [PMID: 34765679] (ELISA Detection, Bacteria) ELISA Detection Bacteria
GS Santiago-S, R Noriega-Ri, E Hernández-, F Valiyeva, B Quiñones-D, ES Villodre, BG Debeb, A Rosado-Alb, PE Vivas-Mejí Targeting Lipocalin-2 in Inflammatory Breast Cancer Cells with Small Interference RNA and Small Molecule Inhibitors International Journal of Molecular Sciences, 2021-08-10;22(16):. 2021-08-10 [PMID: 34445288] (Western Blot, Human) Western Blot Human
P Szodoray, TK Andersen, J Heinzelbec, JF Imbery, PC Huszthy, SM Stanford, B Bogen, OB Landsverk, N Bottini, A Tveita, LA Munthe, B Nakken Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity Cell Reports, 2021-08-10;36(6):109525. 2021-08-10 [PMID: 34380042] (Co-Immunoprecipitation, Human) Co-Immunoprecipitation Human
Show All 50 Publications.

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Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

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CD16 - Find me on macrophages, neutrophils and NK cells
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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Gene Symbol IGHG1