Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.

Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Gt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Donkey
• Conjugate: HRP (Horseradish Peroxidase)

Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

• Immunogen: Goat IgG
• Specificity: Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Donkey
• Gene: IGHG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western
  • Western Blot

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a 0.2 um filtered solution in PBS with stabilizers.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (HAF109)(50)

Publications using HAF109

• Shrestha, M;Wang, DY;Ben-David, Y;Zacksenhaus, E; CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer Oncogenesis 2023-05-26 [PMID: 37230983] (Western Blot, Mouse)
• N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice Frontiers in Physiology, 2023-01-04;13(0):1061715. 2023-01-04 [PMID: 36685192] (Western Blot, Mouse, N/A)
• G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells International Journal of Molecular Sciences, 2022-10-22;23(21):. 2022-10-22 [PMID: 36361539] (Western Blot, Human)
• NC Hait, A Maiti, R Wu, VL Andersen, CC Hsu, Y Wu, DG Chapla, K Takabe, ME Rusiniak, W Bshara, J Zhang, KW Moremen, JTY Lau Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness Cancer Gene Therapy, 2022-06-08;0(0):. 2022-06-08 [PMID: 35676533] (Western Blot, Human)
• D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice Cellular and Molecular Gastroenterology and Hepatology, 2022-06-02;0(0):. 2022-06-02 [PMID: 35660025] (Western Blot, Mouse)
• HL Deissler, M Rehak, A Wolf Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A165 No Longer Depends on the Growth Factor's Presence Biomolecules, 2022-05-23;12(5):. 2022-05-23 [PMID: 35625661] (Western Blot, Bovine)
• J Sidor, M Gillette, LA Dezi, G Untiveros, L Strizzi Role of Presenilin-1 in Aggressive Human Melanoma International Journal of Molecular Sciences, 2022-04-28;23(9):. 2022-04-28 [PMID: 35563300] (Western Blot, Human)
• Z Liu, Y Li, Y Wang, Z Liu, Y Su, Q Ma, R Han Lipoprotein(a), an Opsonin, Enhances the Phagocytosis of Nontypeable Haemophilus influenzae by Macrophages Journal of Immunology Research, 2021-11-02;2021(0):2185568. 2021-11-02 [PMID: 34765679] (ELISA Detection, Bacteria)
• GS Santiago-S, R Noriega-Ri, E Hernández-, F Valiyeva, B Quiñones-D, ES Villodre, BG Debeb, A Rosado-Alb, PE Vivas-Mejí Targeting Lipocalin-2 in Inflammatory Breast Cancer Cells with Small Interference RNA and Small Molecule Inhibitors International Journal of Molecular Sciences, 2021-08-10;22(16):. 2021-08-10 [PMID: 34445288] (Western Blot, Human)
• P Szodoray, TK Andersen, J Heinzelbec, JF Imbery, PC Huszthy, SM Stanford, B Bogen, OB Landsverk, N Bottini, A Tveita, LA Munthe, B Nakken Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity Cell Reports, 2021-08-10;36(6):109525. 2021-08-10 [PMID: 34380042] (Co-Immunoprecipitation, Human)
• A Chrysantho, E Gkaliagkou, A Lazaridis, S Arelaki, P Pateinakis, M Ntinopoulo, A Mitsios, C Antoniadou, C Argyriou, GS Georgiadis, V Papadopoul, A Giatromano, K Ritis, P Skendros Angiotensin II triggers neutrophil extracellular traps release linking thromboinflammation with essential hypertension JCI Insight, 2021-09-22;0(0):. 2021-09-22 [PMID: 34324440] (ELISA Detection, Human)
• A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus Nature Communications, 2021-06-18;12(1):3739. 2021-06-18 [PMID: 34145258] (Western Blot, Mouse)
• J Frew, HB Nygaard Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021-04-01;9(1):57. 2021-04-01 [PMID: 33795008] (IHC, Western Blot, Mouse)
• J Baggen, L Persoons, E Vanstreels, S Jansen, D Van Loover, B Boeckx, V Geudens, J De Man, D Jochmans, J Wauters, E Wauters, BM Vanaudenae, D Lambrechts, J Neyts, K Dallmeier, HJ Thibaut, M Jacquemyn, P Maes, D Daelemans Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2 Nature Genetics, 2021-03-08;0(0):. 2021-03-08 [PMID: 33686287] (Western Blot, Human)
• N Burrows, RJM Bashford-R, VJ Bhute, A Peñalver, JR Ferdinand, BJ Stewart, JEG Smith, M Deobagkar-, G Giudice, TM Connor, A Inaba, L Bergamasch, S Smith, MGB Tran, E Petsalaki, PA Lyons, M Espeli, BJP Huntly, KGC Smith, RJ Cornall, MR Clatworthy, PH Maxwell Dynamic regulation of hypoxia-inducible factor-1&alpha activity is essential for normal B cell development Nat. Immunol., 2020-08-31;0(0):. 2020-08-31 [PMID: 32868930] (Mouse)
• A Göbel, VM Zinna, S Dell'Endic, N Jaschke, JD Kuhlmann, P Wimberger, TD Rachner Anti-tumor effects of mevalonate pathway inhibition in ovarian cancer BMC Cancer, 2020-07-29;20(1):703. 2020-07-29 [PMID: 32727400] (Western Blot, Human)
• Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice Mol. Ther., 2020-07-10;0(0):. 2020-07-10 [PMID: 32652045] (Western Blot, Human)
• T Toyonaga, EC Steinbach, BP Keith, JB Barrow, MR Schaner, EA Wolber, C Beasley, J Huling, Y Wang, NL Allbritton, N Chaumont, TS Sadiq, MJ Koruda, A Jain, MD Long, EL Barnes, HH Herfarth, KL Isaacs, JJ Hansen, MT Shanahan, R Rahbar, TS Furey, P Sethupathy, SZ Sheikh Decreased Colonic Activin Receptor-Like Kinase 1 Disrupts Epithelial Barrier Integrity in Patients with Crohn's disease Cell Mol Gastroenterol Hepatol, 2020-06-16;0(0):. 2020-06-16 [PMID: 32561494] (Western Blot, Human)
• RM Gorczynski, T Maqbool, G Hoffmann Combined IMIG and Immune Ig Attenuate Allergic Responses in Beagle Dogs J Immunol Res, 2020-04-21;2020(0):2061609. 2020-04-21 [PMID: 32377529] (ELISA Development, Canine)
• K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions J Neuroinflammation, 2020-05-02;17(1):141. 2020-05-02 [PMID: 32359362] (Western Blot, Rat)
• HC Huang, MH Tsai, FY Lee, TY Lin, CC Chang, CL Chuang, SJ Hsu, MC Hou, YH Huang NAFLD Aggravates Septic Shock Due to Inadequate Adrenal Response and 11beta-HSDs Dysregulation in Rats Pharmaceutics, 2020-04-28;12(5):. 2020-04-28 [PMID: 32354071] (Western Blot, Rat)
• L Zhou, H Sui, T Wang, R Jia, Z Zhang, J Fu, Y Feng, N Liu, Q Ji, Y Wang, B Zhang, Q Li, Y Li Tanshinone IIA reduces secretion of pro?angiogenic factors and inhibits angiogenesis in human colorectal cancer Oncol. Rep., 2020-02-12;0(0):. 2020-02-12 [PMID: 32323837] (Western Blots, Human)
• JH Kim, IR Lim, CY Park, HJ Joo, JM Noh, SC Choi, SJ Hong, DS Lim Thymosin &beta4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model Int J Mol Sci, 2020-03-21;21(6):. 2020-03-21 [PMID: 32245208] (Western Blot, Human)
• S Saleem, S Tariq, I Aleem, Sadr-Ul Sh, M Tahseen, A Atiq, S Hassan, M Abu Bakar, S Khattak, AA Syed, AH Ahmad, M Hussain, MA Yusuf, C Sutton Proteomics analysis of colon cancer progression Clin Proteomics, 2019-12-28;16(0):44. 2019-12-28 [PMID: 31889941] (Western Blot, Human)
• BN Corder, BL Bullard, JL DeBeaucham, NA Ilyushina, RJ Webby, EA Weaver Influenza H1 Mosaic Hemagglutinin Vaccine Induces Broad Immunity and Protection in Mice Vaccines (Basel), 2019-11-23;7(4):. 2019-11-23 [PMID: 31771231] (Western Blot, Mouse)
• L He, W Tai, J Li, Y Chen, Y Gao, J Li, S Sun, Y Zhou, L Du, G Zhao Enhanced Ability of Oligomeric Nanobodies Targeting MERS Coronavirus Receptor-Binding Domain Viruses, 2019-02-19;11(2):. 2019-02-19 [PMID: 30791410] (Western Blot, Virus)
• LE Bazzone, M King, CR MacKay, PP Kyawe, P Meraner, D Lindstrom, J Rojas-Quin, CA Owen, JP Wang, AL Brass, EA Kurt-Jones, RW Finberg A Disintegrin and Metalloproteinase 9 Domain (ADAM9) Is a Major Susceptibility Factor in the Early Stages of Encephalomyocarditis Virus Infection MBio, 2019-02-05;10(1):. 2019-02-05 [PMID: 30723129] (Western Blot, Human)
• A Göbel, D Breining, M Rauner, LC Hofbauer, TD Rachner Induction of 3-hydroxy-3-methylglutaryl-CoA reductase mediates statin resistance in breast cancer cells Cell Death Dis, 2019-01-28;10(2):91. 2019-01-28 [PMID: 30692522]
• C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells PLoS ONE, 2018-12-07;13(12):e0208774. 2018-12-07 [PMID: 30532256] (Western Blot, Human)
• R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo Genome Biol., 2018-09-19;19(1):137. 2018-09-19 [PMID: 30231914] (Secondary Antibody, Mouse)
• RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain PLoS ONE, 2017-10-10;12(10):e0186317. 2017-10-10 [PMID: 29016674] (Flow Cytometry, Mouse)
• MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity Nat Commun, 2017-09-15;8(1):552. 2017-09-15 [PMID: 28916756] (Western Blot, Human)
• Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix Stem Cell Res Ther, 2017-08-14;8(1):189. 2017-08-14 [PMID: 28807034] (Western Blot, Mouse)
• IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells Int J Mol Sci, 2017-01-22;18(1):. 2017-01-22 [PMID: 28117756] (Western Blot, Human)
• Filomena Gabriella Fulcoli Rebalancing gene haploinsufficiency in vivo by targeting chromatin Nat Commun, 2016-06-03;7(0):11688. 2016-06-03 [PMID: 27256596] (Western Blot, Mouse)
• Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation. Sci Rep, 2015-04-15;5(0):9675. 2015-04-15 [PMID: 25872908] (Bioassay, Human)
• Khalkhali-Ellis , Zhila, Goossens , William, Margaryan , Naira V, Hendrix , Mary J C Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland. PLoS ONE, 2014-07-23;9(7):e103230. 2014-07-23 [PMID: 25054204] (IHC-Fr, Human)
• Yamashita , Yasunobu, Ueyama , Takashi, Nishi , Toshio, Yamamoto , Yuta, Kawakoshi , Akatsuki, Sunami , Shogo, Iguchi , Mikitaka, Tamai , Hideyuki, Ueda , Kazuki, Ito , Takao, Tsuruo , Yoshihir, Ichinose , Masao Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole. PLoS ONE, 2014-05-20;9(5):e97419. 2014-05-20 [PMID: 24846271] (Western Blot, Rat)
• Shahrabi-Farahani , Shokoufe, Wang , Lili, Zwaans , Bernadet, Santana , Jeans M, Shimizu , Akio, Takashima , Seiji, Kreuter , Michael, Coultas , Leigh, D'Amore , Patricia, Arbeit , Jeffrey, Akslen , Lars A, Bielenberg , Diane R Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Lab Invest, 2014-05-05;94(7):752-65. 2014-05-05 [PMID: 24791743] (Western Blot, Mouse)
• Rose , Shannon, Frye , Richard, Slattery , John, Wynne , Rebecca, Tippett , Marie, Pavliv , Oleksand, Melnyk , Stepan, James , S Jill Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort. PLoS ONE, 2014-01-08;9(1):e85436. 2014-01-08 [PMID: 24416410] (Western Blot, Human)
• Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum. J Immunol, 2013-03-01;190(7):3427-37. 2013-03-01 [PMID: 23455499] (Western Blot, Mouse)
• Lin CE, Chen SU, Lin CC Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells. PLoS ONE, 2012-07-20;7(7):e41096. 2012-07-20 [PMID: 22911748] (Western Blot, Human)
• Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression. Biochem. Biophys. Res. Commun., 2011-01-26;405(4):521-6. 2011-01-26 [PMID: 21276420] (Western Blot, Mouse)
• Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. PLoS ONE, 2010-09-24;5(9):e12995. 2010-09-24 [PMID: 20886049] (Western Blot, Mouse)
• Faller EM, Sugden SM, McVey MJ Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation. J. Immunol., 2010-07-26;185(5):2854-66. 2010-07-26 [PMID: 20660706] (Western Blot, Human)
• Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Cancer Res., 2009-03-24;69(7):2996-3003. 2009-03-24 [PMID: 19318572] (Western Blot, Human)
• Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s. Biochem. Biophys. Res. Commun., 2009-01-22;380(1):183-7. 2009-01-22 [PMID: 19167362] (Western Blot, Human)
• Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages. Atherosclerosis, 2008-11-17;204(2):405-11. 2008-11-17 [PMID: 19108834] (Western Blot, Mouse)
• Bergen JM, Pun SH Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein. Mol. Pharm., 2007-01-01;4(1):119-28. 2007-01-01 [PMID: 17274669] (ELISA Development, Human)
• Rouy D, Ernens I, Jeanty C, Wagner DR Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation. Anal. Biochem., 2005-03-15;338(2):294-8. 2005-03-15 [PMID: 15745750] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IGHG1