IL-1 beta/IL-1F2 Antibody [Unconjugated]

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑1 beta /IL‑1F2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

IL-1 beta /IL-1F2 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe (Catalog # 310361) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND50 is typically 0.004-0.02 µg/mL.

IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-HumanIL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, B/N, Dual ISH-IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-1 beta /IL-1F2
  Ala117-Ser269
  Accession # NP_000567
• Specificity: Detects human IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse IL‑1 beta, recombinant porcine IL-1 beta, recombinant rat IL-1 beta, recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant cotton rat IL-1 beta, recombinant feline IL‑1 beta, and recombinant guinea pig IL-1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Dxxxxxxxxxxxxxxx 1-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (AF-201-NA)(28)

Publications using AF-201-NA

• C De Witte, K Demeyere, S De Bruycke, B Taminiau, G Daube, R Ducatelle, E Meyer, F Haesebrouc Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration Vet. Res., 2019;50(1):39. 2019 [PMID: 31126330] (Western Blot, Human)
• PJ Goddard, J Sanchez-Ga, SL Slater, M Kalyan, D Ruano-Gall, O Marchès, LÁ Fernández, G Frankel, AR Shenoy Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages Cell Rep, 2019;27(4):1008-1017.e6. 2019 [PMID: 31018119] (Western Blot, Human)
• S Botto, J Abraham, N Mizuno, K Pryke, B Gall, I Landais, DN Streblow, KJ Fruh, VR DeFilippis Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1? Protein during Virion-Mediated Activation of the AIM2 Inflammasome MBio, 2019;10(1):. 2019 [PMID: 30755509] (Western Blot, Human)
• N Nejati Moh, E Bjørkøy Ta, L Ryan, T Espevik, V Boyartchuk ROR? controls inflammatory state of human macrophages PLoS ONE, 2018;13(11):e0207374. 2018 [PMID: 30485323] (Western Blot, Human)
• L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes Nat Commun, 2018;9(1):4260. 2018 [PMID: 30323213]
• L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis Autophagy, 2017;0(0):0. 2017 [PMID: 28933598] (Neutralization, Human)
• JH Jeong, J Kim, J Kim, HR Heo, JS Jeong, YJ Ryu, Y Hong, SS Han, SH Hong, SJ Lee, WJ Kim ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells Tuberc Respir Dis (Seoul), 2017;80(3):247-254. 2017 [PMID: 28747957] (Western Blot, Human)
• HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition J. Exp. Med., 2017;0(0):. 2017 [PMID: 28450382] (Neutralization, Human)
• K Hendrawan, CM Whittingto, MC Brandley, K Belov, MB Thompson The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii J. Exp. Zool. B Mol. Dev. Evol, 2017;0(0):. 2017 [PMID: 28296138] (Western Blot, Lizard)
• 17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome Sci Rep, 2016;6(0):37625. 2016 [PMID: 27883019] (Western Blot, Human)
• Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation J Immunol, 2016;0(0):. 2016 [PMID: 27521339] (Western Blot, Mouse)
• Yong Hwan Park Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS Nat Immunol, 2016;0(0):. 2016 [PMID: 27270401] (Western Blot, Human)
• Edye M, Brough D, Allan S Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage. J Biol Chem, 2015;290(42):25374-81. 2015 [PMID: 26324708] (Western Blot, Human)
• Kortmann J, Brubaker S, Monack D Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin. J Immunol, 2015;195(3):815-9. 2015 [PMID: 26109648] (Western Blot, Human)
• Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages. J Immunol, 2015;194(11):5472-87. 2015 [PMID: 25917098] (Western Blot, Human)
• Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases. Nat Med, 2015;21(3):248-55. 2015 [PMID: 25686105] (Western Blot, Mouse)
• Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha. Eur J Immunol, 2015;45(4):1092-102. 2015 [PMID: 25545357] (Bioassay, Human)
• Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G Methylsulfonylmethane inhibits NLRP3 inflammasome activation. Cytokine, 2015;71(2):223-31. 2015 [PMID: 25461402] (Western Blot, Human)
• An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M Complement C5a potentiates uric acid crystal-induced IL-1beta production. Eur J Immunol, 2014;44(12):3669-79. 2014 [PMID: 25229885] (Western Blot, Human)
• Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects. J Immunol Res, 2014;2014(0):593562. 2014 [PMID: 24741609] (Functional Assay, Human)
• L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages. J Lipid Res, 2013;54(11):2998-3008. 2013 [PMID: 24006511] (Western Blot, Human)
• Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature, 2012;492(7427):123-7. 2012 [PMID: 23143333] (Western Blot, Mouse)
• Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. J. Immunol., 2011;186(7):4175-82. 2011 [PMID: 21368235] (Neutralization, Human)
• Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo. Cancer Res., 2008;68(22):9541-50. 2008 [PMID: 19010931] (Neutralization, Human)
• Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload. Cytokine, 2008;44(3):352-60. 2008 [PMID: 19008121] (IHC-P, Canine)
• Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians. Biol. Reprod., 2007;76(6):1009-15. 2007 [PMID: 17314310] (Western Blot, Amphibian)
• Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo. Photochem. Photobiol., 2004;80(2):231-5. 2004 [PMID: 15362934] (IHC, Human)
• Sengupta J, Dhawan L, Ghosh D Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey. Cytokine, 2003;24(6):277-85. 2003 [PMID: 14609569] (IHC-P, Primate - Macaca mulatta (Rhesus Macaque))

Reviews for IL-1 beta/IL-1F2 Antibody (AF-201-NA) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Anonymous WB Human 02/19/2020

Summary

• Application: Western Blot
• Sample Tested: KO cells
• Species: Human
• Lot: N/A

reviewed by: Anonymous WB Human 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24006511
• Species: Human

Blocking

• Blocking Details: See PMID 24006511

Details

• Detection Notes: See PMID 24006511

Comments

• Comments: Published: See PMID 24006511

reviewed by: Anonymous WB Human 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23221073
• Species: Human

Blocking

• Blocking Details: See PMID 23221073

Details

• Detection Notes: See PMID 23221073

Comments

• Comments: Published: See PMID 23221073

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • NP_000567()