CDK2 Antibody (AN4.3) - BSA Free

Images

 
Western Blot: CDK2 Antibody (AN4.3) [NB100-2721] - Lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, HEK293T human embryonic kidney cell line, and C6 rat ...read more
Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (AN4.3) [NB100-2721] - CDK2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 1:100 dilution of anti-CDK2 mouse monoclonal antibody ...read more
Immunohistochemistry-Paraffin: CDK2 Antibody (AN4.3) [NB100-2721] - CDK2 was detected in human prostate cancer sections using mouse anti-human CDK2 antibody (NB100-2721) followed by incubation with Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
AN4.3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

CDK2 Antibody (AN4.3) - BSA Free Summary

Immunogen
Human recombinant Cdk2
Epitope
The epitope is at the C-terminus.
Localization
nuclear
Specificity
Specific for Cdk2
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDK2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
This antibody is Cytof ready.
Control
HeLa Nuclear Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
NB100-2721 in the following applications:

Publications
Read Publication using
NB100-2721 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CDK2 Antibody (AN4.3) - BSA Free

  • cdc2-related protein kinase
  • CDK2
  • cell devision kinase 2
  • Cell division protein kinase 2
  • cyclin-dependent kinase 2
  • EC 2.7.11
  • EC 2.7.11.22
  • p33 protein kinase
  • p33(CDK2)

Background

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDK2 Antibody (NB100-2721)(1)

We have publications tested in 1 confirmed species: Xenopus.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for CDK2 Antibody (NB100-2721) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2721:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Haiquan Lu
WB Human 10/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDK2 Antibody (NB100-2721) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CDK2 Antibody (NB100-2721)

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Blogs on CDK2.

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Recent Reviews

3
5
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3
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1
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Haiquan Lu
10/04/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDK2
Entrez