IkB-alpha Antibody (6A920)

Images

 
Genetic Strategies: Knockout Validated: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic ...read more
Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical ...read more
Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (6A920) [NB100-56507] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [NB100-56507] - Human breast probed with IkBa antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by ...read more
Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on NIH3T3 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.
Biological Strategies: Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome ...read more
Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red ...read more
Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on Hek293 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Simple Western: IkB-alpha Antibody (6A920) [NB100-56507] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
Clone
6A920
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

IkB-alpha Antibody (6A920) Summary

Immunogen
A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
Specificity
A 40 kDa band is observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Simple Western 1:20
  • Flow Cytometry 0.25-1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1 ug/ml
  • CyTOF-ready
  • Knockout Validated
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. This antibody is CyTOF ready.
Control
IkB-alpha Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-56507 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    21 publications

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IkB-alpha Antibody (6A920)

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

IkBa Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses IkBa becomes phosphorylated, promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for IkB-alpha Antibody (NB100-56507)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
IP
(1)
WB
(21)
All Applications
Filter By Species
Human
(8)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB100-56507 Applications Species
Zhu F, Willette-Brown J, Song NY et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. Apr 12 2017 [PMID: 28407484]
Tomasi ML, Ramani K, Ryoo M. Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response. Am. J. Pathol. Sep 1 2016 [PMID: 27561301] (WB) WB
Gao Q, Liu Y, Wu Y et al. IL-17 intensifies IFN-gamma-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-kB Int. J. Mol. Med. 2015 Dec 11 [PMID: 26677135] (WB, Mouse) WB Mouse
Hochrainer K, Pejanovic N, Olaseun VA et al. The ubiquitin ligase HERC3 attenuates NF-kB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation. Nucleic Acids Res. 2015 Oct 17 [PMID: 26476452] (WB, Human) WB Human
Ikeno H, Apel M, Zouboulis C et al. L-Ascorbyl-2-phosphate attenuates NF-kB signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion Arch. Dermatol. Res. 2015 Apr 18 [PMID: 25894228] (WB, Human) WB Human
Yang XY, Zhao N, Liu YY et al. Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury. Evid Based Complement Alternat Med 2013 [PMID: 23840265] (IHC-P, Rat) IHC-P Rat
Tabit CE, Shenouda SM, Holbrook M et al. Protein Kinase C-beta Contributes to Impaired Endothelial Insulin Signaling in Humans With Diabetes Mellitus Circulation 2013 Jan 1 [PMID: 23204109] (ICC/IF, Human) ICC/IF Human
Pejanovic N, Hochrainer K, Liu T et al. Regulation of nuclear factor kB (NF-kB) transcriptional activity via p65 acetylation by the chaperonin containing TCP1 (CCT). PLoS One. 2012 [PMID: 22860050]
Delhase M, Kim SY, Lee H et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012 Jan 24 [PMID: 22203995]
Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway. Mol Pharmacol. 2010 May [PMID: 20103608]
Show All 33 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IkB-alpha Antibody (NB100-56507) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56507AF350
Alexa Fluor 405 NB100-56507AF405
Alexa Fluor 488 NB100-56507AF488
Alexa Fluor 532 NB100-56507AF532
Alexa Fluor 594 NB100-56507AF594
Alexa Fluor 647 NB100-56507AF647
Alexa Fluor 700 NB100-56507AF700
Alexa Fluor 750 NB100-56507AF750
Allophycocyanin NB100-56507APC
Allophycocyanin/Cy7 NB100-56507APCCY7
Biotin NBP2-24815
DyLight 350 NB100-56507UV
DyLight 405 NB100-56507V
DyLight 488 NB100-56507G
DyLight 550 NB100-56507R
DyLight 594 NB100-56507DL594
DyLight 650 NB100-56507C
DyLight 680 NB100-56507FR
DyLight 755 NB100-56507IR
FITC NB100-56507F
HRP NB100-56507H
Janelia Fluor 549 NB100-56507JF549
Janelia Fluor 646 NB100-56507JF646
PE NB100-56507PE
PE/Atto594 NB100-56507PEATT594
PE/Cy5.5 NB100-56507PECY55
PE/Cy7 NB100-56507PECY7
PerCP NB100-56507PCP

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Blogs on IkB-alpha.

Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

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Bioinformatics

Gene Symbol NFKBIA
Entrez
Uniprot