IkB-alpha Antibody (6A920) - BSA Free


Genetic Strategies: Knockout Validated: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic ...read more
Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical ...read more
Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (6A920) [NB100-56507] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [NB100-56507] - Analysis of a FFPE tissue section of human breast using 1:200 dilution of IkB-alpha clone 6A920 antibody. The staining was developed using HRP ...read more
Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on NIH3T3 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.
Biological Strategies: Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome ...read more
Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red ...read more
Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on Hek293 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Simple Western: IkB-alpha Antibody (6A920) [NB100-56507] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO
BSA Free
1.0 mg/ml

Order Details

IkB-alpha Antibody (6A920) - BSA Free Summary

Partial recombinant protein corresponding to amino acid residues 32-291 of human IkB-alpha.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.25-1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1 ug/ml
  • Knockout Validated
  • Simple Western 1:20
  • Western Blot 1-2 ug/ml
Application Notes
A 40 kDa band is observed.
Read Publications using
NB100-56507 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    27 publications

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for IkB-alpha Antibody (6A920) - BSA Free

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor


IkBa Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses IkBa becomes phosphorylated, promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
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Publications for IkB-alpha Antibody (NB100-56507)(40)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB100-56507 Applications Species
Zeng L, Herdman DS, Lee SM et al. Loss of cAMP signaling in CD11c immune cells protects against diet-induced obesity Diabetes 2023-05-31 [PMID: 37257047] (WB) WB
Ohta S, Asanoma M, Irie N et al. Soy Phospholipids Exert a Renoprotective Effect by Inhibiting the Nuclear Factor Kappa B Pathway in Macrophages Metabolites 2022-04-06 [PMID: 35448517] (WB) WB
Stanfield BA, Purves T, Palmer S, et al. IL-10 and class 1 histone deacetylases act synergistically and independently on the secretion of proinflammatory mediators in alveolar macrophages PloS one 2021-01-20 [PMID: 33471802] (WB, Mouse) WB Mouse
Korgaonkar AA, Nguyen S, Li Y et al. Distinct cellular mediators drive the Janus faces of toll-like receptor 4 regulation of network excitability which impacts working memory performance after brain injury Brain Behav. Immun. 2020-04-04 [PMID: 32259563] (WB, Rat) WB Rat
Kamohara A, Hirata H et al. IgG immune complexes with Staphylococcus aureus protein A enhance osteoclast differentiation and bone resorption by stimulating Fc receptors and TLR2. Int Immunol 2019-12-11 [PMID: 31713625] (WB, Mouse) WB Mouse
Chen Z, Yao L, Liu Y et al. Astragaloside IV regulates NF-kappa B mediated cellular senescence and apoptosis of hepatic stellate cells to suppress PDGF BB induced activation Exp Ther Med 2019-09-25 [PMID: 31641375] (WB, Rat) WB Rat
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)

Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
Zhu F, Willette-Brown J, Song NY et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. 2017-04-12 [PMID: 28407484]
Tomasi ML, Ramani K, Ryoo M. Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response. Am. J. Pathol. 2016-09-01 [PMID: 27561301] (WB) WB
Gao Q, Liu Y, Wu Y et al. IL-17 intensifies IFN-gamma-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-kB Int. J. Mol. Med. 2015-12-11 [PMID: 26677135] (WB, Mouse) WB Mouse
Show All 40 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Blogs on IkB-alpha.

Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

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Gene Symbol NFKBIA