IkB-alpha Antibody (6A920)

Images

 
Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical ...read more
Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [NB100-56507] - Formalin-fixed, paraffin-embedded human breast probed with IkBa antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of ...read more
Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red ...read more
Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.
Biological Strategies: Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome ...read more
Simple Western: IkB-alpha Antibody (6A920) [NB100-56507] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
6A920
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

IkB-alpha Antibody (6A920) Summary

Immunogen
A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
Specificity
A 40 kDa band is observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Simple Western 1:20
  • Flow Cytometry 0.25-1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1 ug/ml
  • CyTOF-ready
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Positive Control
IkB-alpha Lysate (NBP2-65827)
Publications
Read Publications using
NB100-56507 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    21 publications

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IkB-alpha Antibody (6A920)

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IkB-alpha Antibody (NB100-56507)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
IP
(1)
WB
(21)
All Applications
Filter By Species
Human
(8)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB100-56507 Applications Species
Zhu F, Willette-Brown J, Song NY et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. Apr 12 2017 [PMID: 28407484]
Tomasi ML, Ramani K, Ryoo M. Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response. Am. J. Pathol. Sep 1 2016 [PMID: 27561301] (WB) WB
Gao Q, Liu Y, Wu Y et al. IL-17 intensifies IFN-gamma-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-kB Int. J. Mol. Med. 2015 Dec 11 [PMID: 26677135] (WB, Mouse) WB Mouse
Hochrainer K, Pejanovic N, Olaseun VA et al. The ubiquitin ligase HERC3 attenuates NF-kB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation. Nucleic Acids Res. 2015 Oct 17 [PMID: 26476452] (WB, Human) WB Human
Ikeno H, Apel M, Zouboulis C et al. L-Ascorbyl-2-phosphate attenuates NF-kB signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion Arch. Dermatol. Res. 2015 Apr 18 [PMID: 25894228] (WB, Human) WB Human
Yang XY, Zhao N, Liu YY et al. Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury. Evid Based Complement Alternat Med 2013 [PMID: 23840265] (IHC-P, Rat) IHC-P Rat
Tabit CE, Shenouda SM, Holbrook M et al. Protein Kinase C-beta Contributes to Impaired Endothelial Insulin Signaling in Humans With Diabetes Mellitus Circulation 2013 Jan 1 [PMID: 23204109] (ICC/IF, Human) ICC/IF Human
Pejanovic N, Hochrainer K, Liu T et al. Regulation of nuclear factor kB (NF-kB) transcriptional activity via p65 acetylation by the chaperonin containing TCP1 (CCT). PLoS One. 2012 [PMID: 22860050]
Delhase M, Kim SY, Lee H et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012 Jan 24 [PMID: 22203995]
Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway. Mol Pharmacol. 2010 May [PMID: 20103608]
Show All 33 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IkB-alpha Antibody (NB100-56507) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56507AF405
Alexa Fluor 488 Labeled NB100-56507AF488
Alexa Fluor 647 Labeled NB100-56507AF647
Alexa Fluor 700 Labeled NB100-56507AF700
Allophycocyanin Labeled NB100-56507APC
Biotin Labeled NBP2-24815
DyLight 350 Labeled NB100-56507UV
DyLight 405 Labeled NB100-56507V
DyLight 488 Labeled NB100-56507G
DyLight 550 Labeled NB100-56507R
DyLight 650 Labeled NB100-56507C
DyLight 680 Labeled NB100-56507FR
DyLight 755 Labeled NB100-56507IR
HRP Labeled NB100-56507H
PE Labeled NB100-56507PE
PerCP Labeled NB100-56507PCP

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Bioinformatics Tool for IkB-alpha Antibody (NB100-56507)

Discover related pathways, diseases and genes to IkB-alpha Antibody (NB100-56507). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IkB-alpha Antibody (NB100-56507)

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Research Areas for IkB-alpha Antibody (NB100-56507)

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Blogs on IkB-alpha.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

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Bioinformatics

Gene Symbol NFKBIA
Entrez
Uniprot