By Hunter Martinez
The flow cytometer is a critical piece of equipment in any immunologist’s arsenal. Flow cytometry allows for the acquisition of 4-6x105 cells per minute, each resolved at the single cell level. Scientists can interrogate protein, gene expression, as well as metabolic state of a cell using a flow cytometer.
The Basics of Cytometry
A cytometer accomplishes this feat using the principle of fluorescence. Fluorescence occurs when light from a laser is absorbed by a fluorophore, and then emitted as longer wavelength light or lower...