Oct-3/4 Antibody [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified ...read more
Alkaline phosphatase (ALPL) and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct‑3/4 ...read more
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at ...read more
Knockdown OGT inhibits proliferation and tumorsphere formation of hepatoma cell through reducing eIF4E expression. A, Huh7 and PLC/PRF/5 cells were infected with control shRNA, OGT shRNA2 alone, or with wild‐type ...read more
Neural induction of hPSCs. (a) Both hPSC culture and neural differentiation were performed on LN521 substrate. Cortical neurons were induced with dual SMAD inhibition, expanded in the presence of FGF2, and matured with ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Oct-3/4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POU5F1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 3 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF1759 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody [Unconjugated]

  • 3-Oct
  • Oct-3/4
  • OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
  • Oct4
  • Otf3g
  • Otf4
  • Pou5f1

Background

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.
  1. Scholer, H.R. et al. (1990) Nature 344:435.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  5. Pesce, M. et al. (2001) Stem Cells 19:271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oct-3/4 Antibody (AF1759)(63)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 9 applications: ChIP, Co-Immunoprecipitation, Differentiation, Flow Cytometry, ICC, ICC/IF, IHC, IHC-Fr, Western Blot.


Filter By Application
ChIP
(6)
Co-Immunoprecipitation
(1)
Differentiation
(2)
Flow Cytometry
(6)
ICC
(22)
ICC/IF
(1)
IHC
(5)
IHC-Fr
(2)
Western Blot
(4)
All Applications
Filter By Species
Human
(33)
Mouse
(7)
Rat
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using AF1759 Applications Species
Hatim, O;Pavlinov, I;Xu, M;Linask, K;Beers, J;Liu, C;Baumgärtel, K;Gilbert, M;Spinner, N;Chen, C;Zou, J;Zheng, W; Generation of an Alagille syndrome (ALGS) patient-derived induced pluripotent stem cell line (TRNDi032-A) carrying a heterozygous mutation (p.Cys682Leufs*7) in the JAG1 gene Stem cell research 2023-10-18 [PMID: 37890331] (ICC, Flow Cytometry, Human) ICC, Flow Cytometry Human
Su, YR;Gu, SM;Liu, YR;Cheng, YQ;Wan, Q;Sang, X;Chen, MH;Liu, WQ;Shi, Q;Liu, C;Liu, Y;Li, CY;Wang, ZC;Wang, XR; Partial cellular reprogramming stably restores the stemness of senescent epidermal stem cells European review for medical and pharmacological sciences 2023-06-01 [PMID: 37401274] (ICC/IF, Mouse) ICC/IF Mouse
Wang, Y;Peng, J;Song, C;Yang, Y;Qin, T; Zinc finger and SCAN domain-containing 18 suppresses the proliferation, self-renewal, and drug resistance of glioblastoma cells Heliyon 2023-06-01 [PMID: 37389038] (Western Blot, Human) Western Blot Human
J Li, Q Zhu, J Cao, Y Liu, Y Lu, Y Sun, Q Li, Y Huang, S Shang, X Bian, C Li, L Zhang, Y Wang, Y Nie, J Fu, W Li, MA Mazid, Y Jiang, W Jia, X Wang, Y Sun, MA Esteban, Q Sun, F Zhou, Z Liu Cynomolgus monkey embryo model captures gastrulation and early pregnancy Cell Stem Cell, 2023-04-06;30(4):362-377.e7. 2023-04-06 [PMID: 37028403] (IHC, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) IHC Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
AA Almad, L Garcia, A Takanohash, A Gagne, W Yang, J Ann McGuir, D French, A Vanderver Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G>A (p.D249N) autosomal dominant mutation in TUBB4A Stem Cell Research, 2023-03-26;69(0):103083. 2023-03-26 [PMID: 37003180] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
Alain Aguirre-Vázquez, Fabiola Castorena-Torres, Beatriz Silva-Ramírez, Katia Peñuelas-Urquides, María Elena Camacho-Moll, Luis A. Salazar-Olivo et al. Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine Stem Cell Research & Therapy 2023-03-16 [PMID: 36927767]
Qing Luo, Han-pin Pui, Jiayu Chen, Leqian Yu, Paulo R. Jannig, Yu Pei et al. Epiblast-like stem cells established by Wnt/ beta -catenin signaling manifest distinct features of formative pluripotency and germline competence Cell Reports 2023-01-31 [PMID: 36848234]
X Liu, Y Ye, L Zhu, X Xiao, B Zhou, Y Gu, H Si, H Liang, M Liu, J Li, Q Jiang, J Li, S Yu, R Ma, S Su, JY Liao, Q Zhao Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation Nature Communications, 2023-01-16;14(1):238. 2023-01-16 [PMID: 36646707] (IHC, Human, Mouse) IHC Human, Mouse
R Yelagandul, K Stecher, M Novatchkov, L Michetti, G Michlits, J Wang, P Hofbauer, G Vainorius, C Pribitzer, L Isbel, S Mendjan, D Schübeler, U Elling, J Brennecke, O Bell ZFP462 safeguards neural lineage specification by targeting G9A/GLP-mediated heterochromatin to silence enhancers Nature Cell Biology, 2023-01-05;0(0):. 2023-01-05 [PMID: 36604593] (ChIP, Mouse) ChIP Mouse
O'Shea TM, Ao Y, Wang S et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice Nature Communications 2022-09-28 [PMID: 36171203]
Show All 63 Publications.

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Bioinformatics

Gene Symbol POU5F1
Uniprot