Oct-3/4 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at ...read more
Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified ...read more
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 ...read more
Alkaline phosphatase (ALPL) and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct‑3/4 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Oct-3/4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POU5F1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 20 ug/mL
  • Immunoprecipitation 3 ug/10^6 cells
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF1759 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody [Unconjugated]

  • 3-Oct
  • Oct-3/4
  • Oct4
  • Otf3g
  • Otf4
  • Pou5f1

Background

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.
  1. Scholer, H.R. et al. (1990) Nature 344:435.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  5. Pesce, M. et al. (2001) Stem Cells 19:271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Oct-3/4 Antibody (AF1759)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 7 applications: ChIP, Differentiation, Flow Cytometry, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ChIP
(3)
Differentiation
(1)
Flow Cytometry
(1)
ICC
(11)
IHC
(1)
IHC-Fr
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(18)
Mouse
(3)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF1759 Applications Species
M Sherman-Sa, H Onallah, A Holth, R Reich, B Davidson SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma Gynecol. Oncol., 2019;0(0):. 2019 [PMID: 30904337] (ICC, Human) ICC Human
B Cao, M Duan, Y Xing, C Liu, F Yang, Y Li, T Yang, Y Wei, Q Gao, J Jiang O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30677218] (Western Blot, Human) Western Blot Human
W Zhang, C Chronis, X Chen, H Zhang, R Spalinskas, M Pardo, L Chen, G Wu, Z Zhu, Y Yu, L Yu, J Choudhary, J Nichols, MM Parast, B Greber, P Sahlén, K Plath The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation Cell Stem Cell, 2019;24(1):138-152.e8. 2019 [PMID: 30609396] (Western Blot, Mouse) Western Blot Mouse
S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep, 2018;8(1):15503. 2018 [PMID: 30341351] (Differentiation, Human) Differentiation Human
A Buskin, L Zhu, V Chichagova, B Basu, S Mozaffari-, D Dolan, A Droop, J Collin, R Bronstein, S Mehrotra, M Farkas, G Hilgen, K White, KT Pan, A Treumann, D Hallam, K Bialas, G Chung, C Mellough, Y Ding, N Krasnogor, S Przyborski, S Zwolinski, J Al-Aama, S Alharthi, Y Xu, G Wheway, K Szymanska, M McKibbin, CF Inglehearn, DJ Elliott, S Lindsay, RR Ali, DH Steel, L Armstrong, E Sernagor, H Urlaub, E Pierce, R Lührmann, SN Grellschei, CA Johnson, M Lako Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa Nat Commun, 2018;9(1):4234. 2018 [PMID: 30315276] (ICC, Human) ICC Human
X Gao, JJ Yourick, RL Sprando Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel Stem Cell Res, 2018;31(0):193-196. 2018 [PMID: 30099336] (ICC, Human) ICC Human
K Alsayegh, K Matsuura, H Sekine, T Shimizu Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue Sci Rep, 2017;7(0):45577. 2017 [PMID: 28361959] (ICC, Human) ICC Human
Brain tissue banking for stem cells for our future Sci Rep, 2016;6(0):39394. 2016 [PMID: 27991551] (IHC, Human) IHC Human
Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9 Stem Cell Res, 2016;17(2):441-443. 2016 [PMID: 27879221] (IHC-Fr, Human) IHC-Fr Human
Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9 Stem Cell Res, 2016;17(2):430-432. 2016 [PMID: 27879218] (IHC-Fr, Human) IHC-Fr Human
Show All 23 Publications.

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Biotin BAF1759

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Gene Symbol POU5F1
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