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Oct-3/4 Antibody [Unconjugated]

Images

 
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 ...read more
Alkaline phosphatase (ALPL) and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct‑3/4 ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at ...read more
Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Oct-3/4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POU5F1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 3 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF1759 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody [Unconjugated]

  • 3-Oct
  • Oct-3/4
  • Oct4
  • Otf3g
  • Otf4
  • Pou5f1

Background

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.
  1. Scholer, H.R. et al. (1990) Nature 344:435.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  5. Pesce, M. et al. (2001) Stem Cells 19:271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Oct-3/4 Antibody (AF1759)(33)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 7 applications: ChIP, Differentiation, Flow Cytometry, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ChIP
(4)
Differentiation
(1)
Flow Cytometry
(3)
ICC
(18)
IHC
(1)
IHC-Fr
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(27)
Mouse
(3)
Rat
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using AF1759 Applications Species
L Pagliaroli, P Porazzi, AT Curtis, C Scopa, HMM Mikkers, C Freund, L Daxinger, S Deliard, SA Welsh, S Offley, CA Ott, B Calabretta, SA Brugmann, GWE Santen, M Trizzino Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B-related neurodevelopmental disorders Nature Communications, 2021;12(1):6469. 2021 [PMID: 34753942] (ICC, Human) ICC Human
L Ma, M Schmidt, EM Morrow Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls Stem Cell Research, 2021;54(0):102435. 2021 [PMID: 34182254] (ICC, Human) ICC Human
MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration Science Advances, 2021;7(21):. 2021 [PMID: 34138734] (Flow Cytometry, Human) Flow Cytometry Human
ME Mor, A Harvey, M Familari, M St Clair-G, S Viventi, RU de Iongh, FJ Cameron, M Dottori Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain Brain research bulletin, 2021;173(0):141-149. 2021 [PMID: 34022288] (ICC, Human) ICC Human
P Grönroos, T Ilmarinen, H Skottman Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions Cells, 2021;10(2):. 2021 [PMID: 33562615] (ICC, Human) ICC Human
SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021;12(1):262. 2021 [PMID: 33431859] (ICC, Human) ICC Human
NQ Liu, M Maresca, T van den Br, L Braccioli, MMGA Schijns, H Teunissen, BG Bruneau, EP Nora, E de Wit WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation Nature Genetics, 2020;0(0):. 2020 [PMID: 33318687] (ChIP, Human) ChIP Human
DZ Hazelbaker, A Beccard, G Angelini, P Mazzucato, A Messana, D Lam, K Eggan, LE Barrett A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells Sci Rep, 2020;10(1):635. 2020 [PMID: 31959800] (ICC, Human) ICC Human
T Hyvärinen, A Hyysalo, FE Kapucu, L Aarnos, A Vinogradov, SJ Eglen, L Ylä-Outine, S Narkilahti Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures Sci Rep, 2019;9(1):17125. 2019 [PMID: 31748598] (ICC, Rat) ICC Rat
F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A Sci Rep, 2019;9(1):8126. 2019 [PMID: 31148575] (Flow Cytometry, Human) Flow Cytometry Human
Show All 33 Publications.

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Bioinformatics

Gene Symbol POU5F1
Uniprot