RelA/NFkB p65 Antibody (112A1021)

Images

 
Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse ...read more
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at ...read more
Flow (Intracellular): RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents ...read more
Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of NF-kB (p65) using NB100-56055 (biotinylated version of Clone 112A1021) at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 ...read more
Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of Biotin conjugate of NB100-56712. OfRelA/NFkB p65 Antibody (112A1021) [Biotin]using NB100-56055 at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) ...read more
Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. P65 using p65 antibody at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell ...read more
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. ...read more
Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. This NFkB p65 ...read more
Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using ...read more
Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, IHC, IHC-P, CyTOF-ready
Clone
112A1021
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

RelA/NFkB p65 Antibody (112A1021) Summary

Immunogen
This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RELA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Simple Western 1:25
  • Flow Cytometry 0.25-0.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
Western Blot and Flow cytometry (intracellular) as reported in the literature (Wittmann et al, 2005). IHC (paraffin) as reported in the literature (Chang et al, 2005).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
NFkB p105/p50 Lysate (NBL1-13619)
Publications
Read Publications using
NB100-56712 in the following applications:

Reactivity Notes

Predicted to react with Cow, Gorilla, New World Monkey and Pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for RelA/NFkB p65 Antibody (112A1021)

  • anti-NF kB p65
  • anti-NFkB p65
  • anti-NF-kB p65
  • anti-p65
  • anti-rela p65
  • anti-RelA
  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65 flow cytometry
  • p65 immunohistochemistry
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NB100-56712)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow-IC, IHC-P, WB.


Filter By Application
Flow-IC
(1)
IHC-P
(4)
WB
(3)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-56712 Applications Species
Blanchard Z, Paul BT, Craft B, ElShamy WM. BRCA1-IRIS inactivation overcomes paclitaxel resistance in triple negative breast cancers Breast Cancer Res. 2015 Jan 13 [PMID: 25583261] (WB, Human) WB Human
Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014 May 23 [PMID: 24854039] (WB, IHC-P, Mouse)

Details:
Liver: WB (Fig 9A); IHC-P (Fig 9B). WB & IHC-P showed that radiation treatment resulted in nuclear translocation of NF-kB. In IHC-P, NF-kB was primarily in the cytoplasm of hepatocytes of the control animals whereas there was a dramatic increase of staine
WB, IHC-P Mouse
Ren S, Fan X, Peng L et al. Expression of NF-kB, CD68 and CD105 in carotid atherosclerotic plaque. J Thorac Dis 2013 Dec [PMID: 24409354] (IHC-P, Human) IHC-P Human
Rasheed Z, Anbazhagan AN, Akhtar N et al. Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-alpha and matrix metalloproteinase-13 in human chondrocytes. Arthritis Res Ther. 2009 [PMID: 19445683]
Lee TL, Yeh J, Friedman J et al. A signal network involving coactivated NF-kappaB and STAT3 and altered p53 modulates BAX/BCL-XL expression and promotes cell survival of head and neck squamous cell carcinomas. Int J Cancer. 2008 May 1 [PMID: 18172861]
Santhi WS, Sebastian P, Varghese BT et al. NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins. Exp Mol Pathol. 2006 Oct [PMID: 16822500] (IHC-P, Human)

Details:
Products used (human): 1. IkBa [IMG-127A]: IHC paraffin (normal oral mucosa, leukoplakia, and oral cancer), Fig. 1 IHC paraffin analysis (normal oral mucosa, leukoplakia, and oral cancer), Fig. 2 IHC paraffin (oral cancer: primary tumor, surgical margin,
IHC-P Human
Chang MS, Lee HS, Jung EJ et al. Cell-cycle regulators, bcl-2 and NF-kappaB in Epstein-Barr virus-positive gastric carcinomas. Int J Oncol. 2005 Nov [PMID: 16211221] (IHC-P, Human)

Details:
NF-kB (p65), IMG-150A: IHC (paraffin), Fig 2F (human gastric carcinoma).
IHC-P Human
Wittmann M, Purwar R, Hartmann C et al. Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases. J Invest Dermatol. 2005 Jun [PMID: 15955098] (Flow-IC, Human)

Details:
Flow (Intracellular), Fig 3 (Intact nuclei isolated from normal human keratinocytes: analysis of NF-kB translocation to the nucleus).
Flow-IC Human
Sikder K, Kesh SB, Das N et al. High antioxidative power of quercetin (aglycone flavonoid) and its glycone (rutin) avert high cholesterol diet induced hepato toxicity and inflammation in Swiss albino mice. Food Function. 2014 Feb 20 (WB, Mouse)

Details:
Liver nuclear extract, Fig 5A.
WB Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RelA/NFkB p65 Antibody (NB100-56712). (Showing 1 - 2 of 2 FAQs).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.
  2. We are interested in your item, NB100-56712 and I found this description, Predicted to react with Cow, Gorilla, New World Monkey and Pig, on your datasheet. I want to blast their identity(100%) by myself but I could not find RelA/NFkB p65 protein sequence for Gorilla. Would you please kindly provide us the gene ID or protein ID for Gorilla RelA?
    • The gorilla RelA protein that was blasted against the immunogen for our NB100-56712 can be found on the following link: Ultiprot

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-27416AF405
Alexa Fluor 488 Labeled NBP2-27416AF488
Alexa Fluor 647 Labeled NBP2-27416AF647
Alexa Fluor 700 Labeled NBP2-27416AF700
Allophycocyanin Labeled NBP2-27416APC
Biotin Labeled NB100-56055
DyLight 350 Labeled NBP2-27416UV
DyLight 405 Labeled NBP2-27416V
DyLight 488 Labeled NBP2-27416G
DyLight 550 Labeled NBP2-27416R
DyLight 650 Labeled NBP2-27416C
DyLight 680 Labeled NBP2-27416FR
DyLight 755 Labeled NBP2-27416IR
HRP Labeled NBP2-27416H
PE Labeled NBP2-27416PE
PerCP Labeled NBP2-27416PCP

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Bioinformatics

Gene Symbol RELA
Entrez