RelA/NFkB p65 Antibody (112A1021)


Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of NF-kB (p65) using NB100-56055 (biotinylated version of Clone 112A1021) at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 more
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. more
Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. This NFkB p65 more
Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, IHC, IHC-P
1.0 mg/ml

RelA/NFkB p65 Antibody (112A1021) Summary

This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
Western Blot and Flow cytometry (intracellular) as reported in the literature (Wittmann et al, 2005). IHC (paraffin) as reported in the literature (Chang et al, 2005). In Simple Western only 10-15 uL of the recommended dilution is used per data point.
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NB100-56712 in the following applications:

Reactivity Notes

Predicted to react with Cow, Gorilla, New World Monkey and Pig.

Alternate Names for RelA/NFkB p65 Antibody (112A1021)

  • anti-NF kB p65
  • anti-NFkB p65
  • anti-NF-kB p65
  • anti-p65
  • anti-rela p65
  • anti-RelA
  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65 flow cytometry
  • p65 immunohistochemistry
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65


NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NB100-56712)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow-IC, IHC-P, WB.

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Showing Publications 1 - 9 of 9.
Publications using NB100-56712 Applications Species
Blanchard Z, Paul BT, Craft B, ElShamy WM. BRCA1-IRIS inactivation overcomes paclitaxel resistance in triple negative breast cancers Breast Cancer Res. 2015 Jan 13 [PMID:25583261] (WB, Human) WB Human
Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014 May 23 [PMID:24854039] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Ren S, Fan X, Peng L et al. Expression of NF-kB, CD68 and CD105 in carotid atherosclerotic plaque. J Thorac Dis 2013 Dec [PMID:24409354] (IHC-P, Human) IHC-P Human
Rasheed Z, Anbazhagan AN, Akhtar N et al. Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-alpha and matrix metalloproteinase-13 in human chondrocytes. Arthritis Res Ther. 2009 [PMID:19445683]
Lee TL, Yeh J, Friedman J et al. A signal network involving coactivated NF-kappaB and STAT3 and altered p53 modulates BAX/BCL-XL expression and promotes cell survival of head and neck squamous cell carcinomas. Int J Cancer. 2008 May 1 [PMID:18172861]
Santhi WS, Sebastian P, Varghese BT et al. NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins. Exp Mol Pathol. 2006 Oct [PMID:16822500] (IHC-P, Human) IHC-P Human
Chang MS, Lee HS, Jung EJ et al. Cell-cycle regulators, bcl-2 and NF-kappaB in Epstein-Barr virus-positive gastric carcinomas. Int J Oncol. 2005 Nov [PMID:16211221] (IHC-P, Human) IHC-P Human
Wittmann M, Purwar R, Hartmann C et al. Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases. J Invest Dermatol. 2005 Jun [PMID:15955098] (Flow-IC, Human) Flow-IC Human
Sikder K, Kesh SB, Das N et al. High antioxidative power of quercetin (aglycone flavonoid) and its glycone (rutin) avert high cholesterol diet induced hepato toxicity and inflammation in Swiss albino mice. Food Function. 2014 Feb 20 (WB, Mouse) WB Mouse

Reviews for RelA/NFkB p65 Antibody (NB100-56712) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RelA/NFkB p65 Antibody (NB100-56712). (Showing 1 - 2 of 2 FAQs).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.
  2. We are interested in your item, NB100-56712 and I found this description, Predicted to react with Cow, Gorilla, New World Monkey and Pig, on your datasheet. I want to blast their identity(100%) by myself but I could not find RelA/NFkB p65 protein sequence for Gorilla. Would you please kindly provide us the gene ID or protein ID for Gorilla RelA?
    • The gorilla RelA protein that was blasted against the immunogen for our NB100-56712 can be found on the following link: Ultiprot

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP2-27416AF405
Alexa Fluor (R) 488 Labeled NBP2-27416AF488
Alexa Fluor (R) 647 Labeled NBP2-27416AF647
Alexa Fluor (R) 700 Labeled NBP2-27416AF700
Allophycocyanin Labeled NBP2-27416APC
Biotin Labeled NB100-56055
DyLight 350 Labeled NBP2-27416UV
DyLight 405 Labeled NBP2-27416V
DyLight 405LS Labeled NBP2-27416V3
DyLight 488 Labeled NBP2-27416G
DyLight 550 Labeled NBP2-27416R
DyLight 650 Labeled NBP2-27416C
DyLight 680 Labeled NBP2-27416FR
DyLight 755 Labeled NBP2-27416IR
HRP Labeled NBP2-27416H
PE Labeled NBP2-27416PE
PerCP Labeled NBP2-27416PCP

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Blogs on RelA/NFkB p65.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol RELA

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