Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of NF-kB (p65) using NB100-56055 (biotinylated version of Clone 112A1021) at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 ...read more
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. ...read more
Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. This NFkB p65 ...read more
Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Western Blot and Flow cytometry (intracellular) as reported in the literature (Wittmann et al, 2005). IHC (paraffin) as reported in the literature (Chang et al, 2005). In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using NB100-56712 in the following applications:
Predicted to react with Cow, Gorilla, New World Monkey and Pig.
Alternate Names for RelA/NFkB p65 Antibody (112A1021)
anti-NF kB p65
nf kb p65
NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
p65 flow cytometry
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65
NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Sikder K, Kesh SB, Das N et al. High antioxidative power of quercetin (aglycone flavonoid) and its glycone (rutin) avert high cholesterol diet induced hepato toxicity and inflammation in Swiss albino mice. Food Function. 2014 Feb 20 (WB, Mouse)
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FAQs for RelA/NFkB p65 Antibody (NB100-56712). (Showing 1 - 2 of 2 FAQs).
Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
These antibodies are all purified from tissue culture supernatant.
We are interested in your item, NB100-56712 and I found this description, Predicted to react with Cow, Gorilla, New World Monkey and Pig, on your datasheet. I want to blast their identity(100%) by myself but I could not find RelA/NFkB p65 protein sequence for Gorilla. Would you please kindly provide us the gene ID or protein ID for Gorilla RelA?
The gorilla RelA protein that was blasted against the immunogen for our NB100-56712 can be found on the following link: Ultiprot
NFkB3-p65: Say that three times fast! NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus... Read full blog post.