Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

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Western Blot: Goat anti-Mouse, Human IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) [NBP1-75144] - Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) [NBP1-75144] - Lysates of Daudi human Burkitt's ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Format
Pre-absorbed
Concentration
LYOPH

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Purified Mouse IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, horse, human, pig, rabbit protein IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry-Paraffin 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP1-75144 in the following applications:

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates.

Notes

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG (H+L) Antibody (NBP1-75144) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-75144:
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NBP1-75144
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reviewed by:
Jim Hsiao
WB Mouse 05/22/2017
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Summary

ApplicationWestern Blot
Sample TestedMouse lung endothelial cells
SpeciesMouse
Lot384903014

Comments

CommentsPrimary antibody: Mouse monoclonal GAPDH (1:2500 in 5% milk/TBST); Secondary: HRP-conjugated goat anti-mouse IgG (1:5000).

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Jim Hsiao
05/22/2017
Application: WB
Species: Mouse