HA Tag Antibody

Images

 
Western Blot: HA Tag Antibody [NB600-363] - Detection of HA-tagged Protein by Western Blot. Samples : 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody : Affinity purified ...read more
Immunocytochemistry/ Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. ...read more
Western Blot: HA Tag Antibody [NB600-363] - WB validation of HA Tag antibody on (1) 293T/17 whole cell lysate and (2) HA-Tag transfected 293T/17 whole cell lysate. The primary antibody was used at 1:5000 dilution and ...read more
Immunocytochemistry/ Immunofluorescence: HA Tag Antibody [NB600-363] - tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA ...read more
Immunoprecipitation: HA Tag Antibody [NB600-363] - Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity ...read more

Product Details

Summary
Reactivity Hu, Ec, NASpecies Glossary
Applications WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HA Tag Antibody Summary

Immunogen
Rabbits were immunized with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
ICC (1:100-1:400), IP (1-4ug/mg lysate), ELISA: coating dilution 1:100-1:500, primary 1:10,00-1:30,000, and for WB: colorimetric detection dilution 1:1,000-1:10,000, chemiluminscent detection dilution 1:1,000-1:30,000. Prepare working dilutions immediately before use. Chromatin Immunoprecipitation and Immunohistochemistry were reported in scientific literature.
Reviewed Applications
Read 2 Reviews rated 4
using
NB600-363 in the following applications:

Publications
Read Publications using
NB600-363 in the following applications:

  • 2 publications
  • 8 publications
  • IHC
    1 publication
  • IP
    3 publications
  • WB
    10 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27203423).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HA Tag Antibody

  • HA Epitope Tag
  • HA Tag
  • HA
  • Hemagglutinin

Background

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (NB600-363)(62)

We have publications tested in 3 confirmed species: Human, Mouse, Xenopus.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC, IP, WB.


Filter By Application
ChIP
(2)
ICC/IF
(8)
IHC
(1)
IP
(3)
WB
(10)
All Applications
Filter By Species
Human
(12)
Mouse
(2)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using NB600-363 Applications Species
Murray AS, Varela FA, Hyland TE et al. Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization J. Biol. Chem. 2017 Jul 14 [PMID: 28710277]
Rissanen I, Ahmed AA, Azarm K et al. Idiosyncratic Mojiang virus attachment glycoprotein directs a host-cell entry pathway distinct from genetically related henipaviruses Nat Commun 2017 Jul 12 [PMID: 28699636] (WB, Human) WB Human
Coleman S, Choi KY, Root M, McGregor A. A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig Cytomegalovirus PLoS Pathog. 2016 Jul 01 [PMID: 27387220] (IP) IP
Park A, Yun T, Vigant F et al. Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway. PLoS Pathog. May 1 2016 [PMID: 27203423] (WB, IP, Human) WB, IP Human
Pentecost M, Vashisht AA, Lester T et al. Evidence for Ubiquitin-Regulated Nuclear and Subnuclear Trafficking among Paramyxovirinae Matrix Proteins. PLoS Pathog. 2015 Mar 01 [PMID: 25782006] (WB, Human) WB Human
Sun S, Ling SC, Qiu J et al. ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP Nat Commun. 2015 Jan 28 [PMID: 25625564] (WB, ICC/IF, Human, Mouse) WB, ICC/IF Human, Mouse
Mussolino C, Alzubi J, Fine EJ et al. TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity. Nucleic Acids Res. 2014 May 03 [PMID: 24792154] (WB) WB
Bobis-Wozowicz S, Galla M, Alzubi J et al. Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery. Sci Rep 2014 Apr 11 [PMID: 24722320] (WB) WB
Smith CJ, McGlade CJ. The ubiquitin ligase RNF126 regulates the retrograde sorting of the cation-independent mannose 6-phosphate receptor. Exp Cell Res. 2013 Nov 23 [PMID: 24275455] (ICC/IF, Human) ICC/IF Human
Chen H, Kazemier HG, de Groote ML, Ruiters MH et al. Induced DNA demethylation by targeting Ten-Eleven Translocation 2 to the human ICAM-1 promoter. Nucleic Acids Res. 2013 Nov 4 [PMID: 24194590] (IP) IP
Show All 62 Publications.

Reviews for HA Tag Antibody (NB600-363) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.
We have 2 reviews tested in 1 application: WB.

Reviews using NB600-363:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Filipa Franquinho
WB Human 05/04/2013
View

Summary

ApplicationWestern Blot
Sample TestedHek 293T cell lysate
SpeciesHuman

Blocking

Blocking Details5% BSA + 0,1% tween

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionAnti-rabbit HRP
Secondary Concentration1:5000

Details

Detection NotesECL
Western Blot HA Tag NB600-363
Enlarge
reviewed by:
Maria Andreou
WB Other 09/26/2011
View

Summary

ApplicationWestern Blot
Sample TestedXenopus laevis inejected embryo Lysates, Sample Amount: 30ug
SpeciesOther
LotA4
CommentsLanes 1,2 and 3 are lysates from embryos injected with the HA-tagged constructs and lanes 4,5 are the negative control lanes that come from non injected embryo lysates.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 1 hour, Blocking Temp: 22 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: 1hr, Incubation Temp: 22C

Details

Detection NotesSpecific Bands: 90kDa, 37kDa, Non-Specific Bands: 100kDa, 50kDa

Comments

CommentsLanes 1,2 and 3 are lysates from embryos injected with the HA-tagged constructs and lanes 4,5 are the negative control lanes that come from non injected embryo lysates.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HA Tag Antibody (NB600-363). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
    • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose Immobilized Labeled NBP1-30434
Biotin Labeled NB600-363B
FITC Labeled NB600-363F
HRP Labeled NB600-391

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Recent Reviews

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Filipa Franquinho
05/04/2013
Application: WB
Species: Human

Maria Andreou
09/26/2011
Application: WB
Species: Other

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