Caspase-3 Antibody (CPP32 4-1-18)

Images

 
Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Western blot detection of Caspase (19 and 35 kDa) from HEK293 cell extract using NB500-210. Lanes 1 and 2 contain inactive and active Caspase, respectively.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - IHC analysis of a formalin fixed and paraffin embedded human spleen section using 1:200 dilution of Caspase-3 antibody (clone CPP32 4-1-18). ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder 1:300 dilution of mouse anti-Caspase-3 monoclonal ...read more
Simple Western: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Simple Western lane view shows a specific band for Caspase 3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clone
CPP32 4-1-18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Caspase-3 Antibody (CPP32 4-1-18) Summary

Immunogen
Full-length recombinant human Caspase 3 [UniProt# P42574]
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 1:2000
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
This Caspase 3 (CPP32 4-1-18) antibody is useful for Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is seen at ~32 kDa for the inactive form of Caspase 3 and ~17-22 kDa for the active form of Caspase 3. (See protocol for additional information).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
293 Lysate (NB800-PC6)
Caspase-3 Lysate (NBL1-08710)
Reviewed Applications
Read 3 Reviews rated 4
using
NB500-210 in the following applications:

Publications
Read Publications using
NB500-210 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    6 publications

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-3 Antibody (CPP32 4-1-18)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 is a cytoplasmic heterotetrameric cysteine protease involved in activating caspases cascade which is responsible for execution of apoptosis. It contains two anti-parallel arranged heterodimers (each one formed by a 17 kD nd a 12 kD subunit) and is involved in the cleavage/activation of caspases cascade (caspase-6, -7 and -9) responsible for apoptosis execution. Caspase-3's cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Caspase-3 undergoes S-nitrosylization on its catalytic site cysteine in unstimulated cells where as it gets denitrosylated upon activation of the Fas apoptotic pathway, associated with an increased intracellular caspase activity. Moreover, at the onset of apoptosis, caspase-3 proteolytically cleaves PARP at a '216-Asp-|-Gly-217' bond, and also cleaves/activates SREBPs between bHLH zipper domain and the membrane attachment domain. It interacts with BIRC6/bruce also and is involved in the cleavage of huntingtin and triggers cell adhesion in sympathetic neurons through RET cleavage. Caspase-3 is an important marker of apoptosis which is implicated in several physiological and pathological processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (NB500-210)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB500-210 Applications Species
Martins FF, Aguila MB, Mandarim-de-Lacerda CA. Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-). Acta Histochem. May 13 2017 [PMID: 28506466]
Graus-Nunes F, Marinho TS, Barbosa-da-Silva S et al. Differential effects of angiotensin receptor blockers on pancreatic islet remodelling and glucose homeostasis in diet-induced obese mice. Mol. Cell. Endocrinol. Oct 22 2016 [PMID: 27780713] (IHC, Mouse) IHC Mouse
Kim H, Lee KH, Park IA et al. Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer. Virchows Arch. 2015 Aug 18 [PMID: 26280894] (IHC-P, Human)

Details:
Caspase 3 antibody was used at 1:300 dilution for IHC-P analysis of tissue sections from human cases of luminal A invasive breast ductal carcinoma. The assay was performed on benchmark automatic immunostaining device and the signal detection was performed using biotinylated anti-mouse secondary antibody -peroxidase-labeled streptavidin - DAB method.
IHC-P Human
Shibayama Y, Kondo T, Ohya H et al. Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients. Oncol Rep 2015 May [PMID: 25759982]
Hsuan SW, Chyau CC, Hung HY et al. The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer Eur J Nutr 2015 Feb 27 [PMID: 25720497] (WB, Human) WB Human
Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (WB, Human) WB Human
Chien SY, Kuo SJ, Chen DR, Su CC. Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl?1 and TCTP and increases that of TNF-alpha and Bax in BxPC?3 pancreatic carcinoma cells. Int J Mol Med 2013 Jul [PMID: 23652631]
Chen YL, Yan MY, Chien SY et al. Sann-Joong-Kuey-Jian-Tang inhibits hepatocellular carcinoma Hep-G2 cell proliferation by increasing TNF-alpha, Caspase-8, Caspase- 3 and Bax but by decreasing TCTP and Mcl-1 expression in vitro. Mol Med Rep 2013 May [PMID: 23525225] (Human) Human
Peng Z, Shuangzhu Y, Yongjie J et al. TNF receptor-associated factor 6 regulates proliferation, apoptosis, and invasion of glioma cells. Mol Cell Biochem 2013 Jan 29 [PMID: 23358926] (WB, Human) WB Human
Fuzio P, Ditonno P, Rutigliano M et al. Regulation of TGF-B1 expression by Androgen Deprivation Therapy of prostate cancer Cancer Letters 2012 May 18 [PMID: 22269108] (IHC, Human) IHC Human
Show All 18 Publications.

Reviews for Caspase-3 Antibody (NB500-210) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 1 application: WB.

Reviews using NB500-210:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 08/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol
 
reviewed by:
Anonymous
WB Human 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Hepatoblastoma
SpeciesHuman
CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in PBST, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Mouse IgG (H+L) HRP, Jackson

Details

Detection NotesDetection Method: GE ImageQuant LAS 4000 mini plus ECL

Comments

CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis
 
reviewed by:
Anonymous
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse

Details

Detection NotesDetection Method: Western Lighting plus ECL

Product General Protocols

View specific protocols for Caspase-3 Antibody (NB500-210): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-3 Antibody (NB500-210). (Showing 1 - 2 of 2 FAQs).

  1. I am looking for a mouse antibody against active caspase 3. This antibody you have is one of the few mouse anti-caspase 3 antibodies that I can find, but the information about it does not specify whether it is against the active form of the protein. I must have an antibody against active caspase 3, can you tell me whether this is the case for your antibody (NB500-210).
    • I have pulled up information about this antibody and it appears that it will detect both the active and non-active forms of the protein. I don't know if you are looking for one that is only specific to the active and will not detect the inactive, or if as long as it detects the active it will work.
  2. Which is the best retrieval buffer for this antibody to IHC? In addition, which is the dilution for it?
    • Antigen retrieval should be performed using citrate buffer at a pH of 6.0. Our protocol for this can be found here: Antigen Retrieval Protocol.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Caspase-3 Antibody (NB500-210)

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Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

 
Anonymous
08/23/2015
Application: WB
Species: Human

 
Anonymous
12/21/2011
Application: WB
Species: Human

 
Anonymous
11/21/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CASP3
Entrez
Uniprot