Mass Cytometry

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CyTOF-ready Antibodies

CyTOF-reported Antibodies

Understanding Mass Cytometry

Flow Cytometry Resources

Flow Cytometry Panel Builder

Mass cytometry, such as CyTOF, is a variation of flow cytometry in which heavy metals, typically lanthanides, are conjugated to antibodies instead of fluorochromes. The unique mass signatures of these stable metal isotopes are detected by time-of-flight mass spectrometry and enable identification of over 40 cellular targets on a single cell.

Advantages Over Fluorescence Flow Cytometry

  • Reduce Spectral Overlap: Low spectral overlap without the need for fluorescence compensation adjustments.
  • Eliminate Autofluorescence Background: Absence of interference from cellular autofluorescence.
  • Improve Molecular Profiling: Simultaneous detection and resolution of more targets than is feasible with conventional flow cytometry, making CyTOF ideal for testing rare or precious samples.

Bio-Techne CyTOF Antibodies

R&D Systems and Novus Biologicals have a wide range of antibodies that are bottled in a CyTOF-ready format, without BSA or other carrier proteins that may interfere with metal conjugation. Additional benefits for established conjugation methods include:

  • Liquid formulation or lyophilized for reconstitution at high concentration and improved conjugation efficiency.
  • No need for a buffer exchange step prior to conjugation.
  • Over 2600 CyTOF-ready antibodies available.
  • Also available conjugated to fluorochromes for fluorescence flow cytometry.

Choose from:

Mass Cytometry Webinar

In the Bio-Techne webinar, “Single-Cell Profiling with Mass Cytometry: An Overview of Technology and Current Research Applications On-Demand”, learn more about CyTOF from a leading expert.

Select Citations of CyTOF-Reported Antibodies

Application of mass cytometry (CyTOF) for functional and phenotypic analysis of natural killer cells. Kay, A. et al. Methods Mol. Biol. (2016). 1441: 13–26. (Catalog # MAB1844 , MAB699, MAB2014, MAB2238, MAB1381)

High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry. Nair, N. et al. Mucosal Immunol. (2016) 9: 68. (Catalog # MAB197 , MAB170 )

Immune profiles to predict response to desensitization therapy in highly HLA-sensitized kidney transplant candidates. Yabu, J.M. et al. PLoS ONE (2016) 11: e0153355. (Catalog # MAB197, MAB20172)

Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. Mei, H. et al. J. Immunol. (2015) 194:2022. (Catalog # MAB197, MAB20172)

Regulation of adaptive NK cells and CD8 T cells by HLA-C correlates with allogeneic hematopoietic cell transplantation and with cytomegalovirus reactivation. Horowitz, A. et al. J. Immunol. (2015) 195:4524. (Catalog # MAB138, MAB1844, MAB2014)