beta-Actin Antibody (AC-15)

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.

Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Simple Western lane view shows a specific band for Beta-Actin in 0.2 mg/ml of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - HS-68 cells.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Immunofluroescence Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta Actin, Clone No. AC-15 (NB600-501).

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.

Immunohistochemistry: beta-Actin Antibody (AC-15) [NB600-501] - Breast, Epithelium 40x.

Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 for 1 hour. Image from verified customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human cell lines (Caco-2, T84, HCT116, HT29, DU145, BPH1, HeLa). Image from verified customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-actin in NIH/3T3 and HEK293T cells using HRP conjugated beta-actin antibody (Cat# NB600-501H). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e, red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-Beta Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.

Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
• Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: AC-15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

beta-Actin Antibody (AC-15) Summary

• Immunogen: slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Epitope: N-terminus of the beta isoform of actin.
• Specificity: In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000 - 1:10000
  • Simple Western 1:25
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives. Use in immunoprecipitation reported in scientific literature (PMID 23647384).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size: Wes/Sally Sue/Peggy Sue.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antibody cross reacts with b-actin expressing cells in carp, leech tissues (Hirudo medicinalis), ground squirrel. Does not cross react with adult cardiac, skeletal muscle, drosophila or amoeba beta actin. Hamster reactivity reported in scientific literature (PMID: 24478435). Mammal reactivity reported in scientific literature (PMID: 25130694). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 15mM Sodium Azide
• Purity: Unpurified

Alternate Names for beta-Actin Antibody (AC-15)

• ACTB
• actin, beta
• actin, cytoplasmic 1
• anti-beta actin
• beta actin (ac-15)
• Beta Actin
• beta cytoskeletal actin
• beta-actin ac 15
• beta-actin ac15
• beta-actin ac-15
• betaActin
• beta-Actin
• BRWS1
• PS1TP5-binding protein 1
• PS1TP5BP1

Background

Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Actin Antibody (NB600-501)(303)

Publications using NB600-501

• Li TN, Wu YJ, Tsai HW et al. Intrahepatic HBV large surface antigen induces hepatocyte hyperploidy via failure of cytokinesis J. Pathol. 43254 [PMID: 29862509] (Human)
• Zhou R, Xu T, Liu X et al. Activation of spinal dorsal horn P2Y13 receptors can promote the expression of IL-1b and IL-6 in rats with diabetic neuropathic pain. J Pain Res Mar 27 2018 [PMID: 29628771] (WB, Rat)
• Galasso C. Marine Biotechnology: a sea of new resources and solutions for ocean and human health. Thesis. Jul 9 1905 (Human)
• Motzko-Soares ACP, Vizin RCL, Martins TMS et al. Thermoregulatory profile of neurodegeneration-induced dementia of the Alzheimer's type using intracerebroventricular streptozotocin in rats Acta Physiol (Oxf) May 2 2018 [PMID: 29719119] (Rat)
• Smith B. Effects of nutrition and ultrasound imaging on the cardiovascular system Thesis. 2014 (WB, Rabbit)
• Ferriero R, Nusco E, De Cegli R et al. Pyruvate dehydrogenase complex and lactate dehydrogenase as targets for therapy of acute liver failure. J. Hepatol. Mar 23 2018 [PMID: 29580866] (WB, Mouse)
• Tsai MS, Wang YH, Lai YY et al. Kaempferol protects against propacetamol-induced acute liver injury through CYP2E1 inactivation, UGT1A1 activation, and attenuation of oxidative stress, inflammation and apoptosis in mice. Toxicol. Lett. Mar 21 2018 [PMID: 29574133] (Mouse)
• Lee BL, Mirrashidi KM, Stowe IB et al. ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages. Sci Rep. Feb 28 2018 [PMID: 29491424] (WB, Mouse)
• Steiner C, Gevaert T, Ganzer R, De Ridder D. Comparative immunohistochemical characterization of interstitial cells in the urinary bladder of human, guinea pig and pig. Histochem. Cell Biol. 2018 Feb 20 [PMID: 29464320] (Human)
• Ye R, Gordillo R, Shao M et al. Intracellular lipid metabolism impairs B cell compensation during diet-induced obesity J. Clin. Invest. Mar 1 2018 [PMID: 29457786] (WB, Mouse)
• Itoh S, Mizuno K, Aikawa M, Aikawa E. Dimerization of sortilin regulates its trafficking to extracellular vesicles. J. Biol. Chem. 2018 Jan 30 [PMID: 29382723] (Human)
• Yu X, Stallone JN, Heaps CL, Han G. The activation of G protein-coupled estrogen receptor induces relaxation via cAMP as well as potentiates contraction via EGFR transactivation in porcine coronary arteries PLoS ONE 2018 Jan 23 [PMID: 29360846] (WB, Porcine)
• Nag S, Resnick A. Stabilization of hypoxia inducible factor by cobalt chloride can alter renal epithelial transport. Physiol Rep 2017 Dec 01 [PMID: 29263117] (WB, Mouse)
• Pol A, Renkema GH, Tangerman A et al. Mutations in SELENBP1, encoding a novel human methanethiol oxidase, cause extraoral halitosis. Nat. Genet. 2018 Jan 01 [PMID: 29255262] (Human)
• Cree BAC, Niu J, Hoi KK et al. Clemastine rescues myelination defects and promotes functional recovery in hypoxic brain injury. Brain 2017 Dec 13 [PMID: 29244098] (WB, Human)
• Ullah K, Rosendahl AH, Izzi V et al. Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-kB and p53 signaling pathways. Sci Rep 2017 Dec 08 [PMID: 29222481] (WB, Mouse)
• Khan N, Muralidharan A, Smith MT. Attenuation of the Infiltration of Angiotensin II Expressing CD3+ T-Cells and the Modulation of Nerve Growth Factor in Lumbar Dorsal Root Ganglia-A Possible Mechanism Underpinning Analgesia Produced by EMA300, An Angiotensin II Type 2 (AT2) Receptor Antag Front Mol Neurosci. 2017 Nov 21 [PMID: 29200998] (WB, Rat)
• Jongen JMJ, van der Waals LM, Trumpi K et al. Downregulation of DNA repair proteins and increased DNA damage in hypoxic colon cancer cells is a therapeutically exploitable vulnerability Oncotarget. 2017 Oct 17 [PMID: 29156796] (WB)
• Mazzoni J, Smith JR, Shahriar S et al. The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels Neuron. 2017 Nov 04 [PMID: 29154126] (Mouse)
• Ge Q, Zhang H, Hou J et al. VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms Mol Med Rep. 2018 Jan 01 [PMID: 29138837] (WB)
• El-Hassan AR, Leung V, Kharfallah F et al. Identification and characterization of a novel chemically induced allele at the planar cell polarity gene Vangl2. Mamm. Genome. 2017 Oct 24 [PMID: 29063958] (WB, Mouse)
• Zhao W, Pferdehirt L, Segatori L. Quantitatively predictable control of cellular protein levels through proteasomal degradation. ACS Synth Biol. 2017 Oct 23 [PMID: 29061039] (WB)
• Walters DK, Arendt BK, Tschumper RC et al. Characterization and use of the novel human multiple myeloma cell line MC-b11/14 to study biological consequences of CRISPR-mediated loss of immunoglobulin a heavy chain. Exp. Hematol. 2017 Oct 10 [PMID: 29030084] (Human)
• Yoon H, Radulovic M, Walters G et al. Protease activated receptor 2 controls myelin development, resiliency and repair Glia 2017 Sep 18 [PMID: 28921694] (Mouse)
• Niu J, Huang D, Zhou R et al. Activation of dorsal horn cannabinoid CB2 receptor suppresses the expression of P2Y12 and P2Y13 receptors in neuropathic pain rats J Neuroinflammation 2017 Sep 12 [PMID: 28899427] (WB, Rat)
• Nagami M, Ito Y, Nagasawa T. Phenethyl isothiocyanate protects against H2O2-induced insulin resistance in 3T3-L1 adipocytes Biosci. Biotechnol. Biochem. 2017 Sep 13 [PMID: 28899227] (Mouse)
• Chiu HY, Tsay YG, Hung SC. Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture Sci Rep 2017 Aug 31 [PMID: 28860574] (WB, Human)
• Bohnsack JP, Patel VK, Morrow AL. Ethanol exposure regulates Gabra1 expression via histone deacetylation at the promoter in cultured cortical neurons J. Pharmacol. Exp. Ther. 2017 Aug 10 [PMID: 28798030]
• Noutsios GT, Willis AL, Ledford JG, Chang EH. Novel role of surfactant protein A in bacterial sinusitis Int Forum Allergy Rhinol 2017 Jul 20 [PMID: 28727907] (Human)
• Lin NH, Messing A, Perng MD. Characterization of a panel of monoclonal antibodies recognizing specific epitopes on GFAP PLoS ONE 2017 Jul 12 [PMID: 28700643] (WB, Human)
• Yoon H, Walters G, Paulsen AR, Scarisbrick IA. Astrocyte heterogeneity across the brain and spinal cord occurs developmentally, in adulthood and in response to demyelination PLoS ONE 2017 Jul 12 [PMID: 28700615] (WB, Mouse)
• Ambroz M, Matouskova P, Skarka A et al. The Effects of Selected Sesquiterpenes from Myrica rubra Essential Oil on the Efficacy of Doxorubicin in Sensitive and Resistant Cancer Cell Lines. Molecules. 2017 Jun 20 [PMID: 28632185] (WB, Human)
• Chang CJ, Lu CC, Lin CS, Martel J. Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice. Int J Obes (Lond). 2018 Feb 01 [PMID: 28630461] (WB, Mouse)
• Wu PK, Wang JY, Chen CF et al. Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity. Stem Cells Transl Med Jun 1 2017 [PMID: 28544661] (WB, Human)
• Balbaa M, Abdulmalek SA, Khalil S. Oxidative stress and expression of insulin signaling proteins in the brain of diabetic rats: Role of Nigella sativa oil and antidiabetic drugs. PLoS ONE May 15 2017 [PMID: 28505155] (WB, Rat)
• Pettersson H, Zarnegar B, Westin A et al. SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo. Sci Rep Apr 24 2017 [PMID: 28439090] (WB, Mouse)
• Wang X, Dasari S, Nowakowski GS et al. Retinoic acid receptor alpha drives cell cycle progression and is associated with increased sensitivity to retinoids in T-cell lymphoma. Oncotarget Apr 18 2017 [PMID: 28412739] (WB, Human)
• Ho TJ, Lee CW, Lu ZY et al. Effects of Electroacupuncture on Methamphetamine-Induced Behavioral Changes in Mice. Evid Based Complement Alternat Med 2017 Mar 16 [PMID: 28400844] (WB, Mouse)
• Lee YS, Kim JY, Oh KS, Chung SW. Fatty acid-binding protein 4 regulates fatty infiltration after rotator cuff tear by hypoxia-inducible factor 1 in mice. J Cachexia Sarcopenia Muscle. Apr 5 2017 [PMID: 28382782] (WB, Mouse)
• Raats DA, Frenkel N, van Schelven SJ et al. CD95 ligand induces senescence in mismatch repair-deficient human colon cancer via chronic caspase-mediated induction of DNA damage. Cell Death Dis. Mar 16 2017 [PMID: 28300842] (WB, Human)
• Piccolo P, Annunziata P, Soria LR et al. Downregulation of HNF-4a and defective zonation in livers expressing mutant Z a1-antitrypsin.. Hepatology. Mar 15 2017 [PMID: 28295475] (IHC, Mouse)
• Chen KC, Hsieh MS, Li YR et al. Study of poly-e-caprolactone membranes for pleurodesis. J. Formos. Med. Assoc. 2017 Feb 16 [PMID: 28215672] (WB, Rabbit)
• Giatromanolaki A, Liousia M, Arelaki S et al. Differential effect of hypoxia and acidity on lung cancer cell and fibroblast metabolism. Biochemistry and Cell Biology. Jan 24 2017 [PMID: 28177758] (WB)
• Wang X, Boddicker RL, Dasari S et al. Expression of p63 protein in anaplastic large cell lymphoma: Implications for genetic subtyping. Hum. Pathol. 2017 Jan 30 [PMID: 28153507] (WB)
• Sansone C, Galasso C, Orefice I et al. The green microalga Tetraselmis suecica reduces oxidative stress and induces repairing mechanisms in human cells Sci Rep Jan 24 2017 [PMID: 28117410] (WB, Human)
• Lin YC, Chao TY, Yeh CT et al. Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis. Arterioscler. Thromb. Vasc. Biol. Nov 10 2016 [PMID: 27834687]
• Chen HR, Yeh YC, Liu CY et al. DDR1 promotes E-cadherin stability via inhibition of integrin-beta1-Src activation-mediated E-cadherin endocytosis. Sci Rep. Nov 8 2016 [PMID: 27824116] (WB, Canine)
• Getun IV, Wu Z, Fallahi M et al. Functional Roles of Acetylated Histone Marks at Mouse Meiotic Recombination Hotspots. Mol. Cell. Biol. Nov 7 2016 [PMID: 27821479] (WB, Mouse)
• Iwata H, Goettsch C, Sharma A et al. PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation. Nat Commun. Oct 31 2016 [PMID: 27796300] (WB, Mouse)
• Zhou TE, Rivera JC, Bhosle VK et al. Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1B. Am. J. Pathol. Oct 18 2016 [PMID: 27768863] (WB)
• Di K, Lomeli N, Wood SD et al. Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma. Oncotarget. Nov 22 2016 [PMID: 27764809] (WB, Human)
• Pradhan S, Mahajan D, Kaur P et al. Scriptaid overcomes hypoxia-induced cisplatin resistance in both wild-type and mutant p53 lung cancer cells. Oncotarget. Nov 1 2016 [PMID: 27708247] (ICC/IF, Human)
• Hu Y, Su H, Liu C et al. DEPTOR is a direct NOTCH1 target that promotes cell proliferation and survival in T-cell leukemia. Oncogene Sep 5 2016 [PMID: 27593934] (WB)
• Erdogan S, Doganlar O, Doganlar ZB et al. The flavonoid apigenin reduces prostate cancer CD44(+) stem cell survival and migration through PI3K/Akt/NF-kB signaling. Life Sci. Aug 25 2016 [PMID: 27569589] (WB)
• Yoon H, Scarisbrick IA. Kallikrein-related peptidase 6 exacerbates disease in an autoimmune model of multiple sclerosis. Biol. Chem. Aug 17 2016 [PMID: 27533119]
• Vanas V, Haigl B, Stockhammer V, Sutterluty-Fall H. MicroRNA-21 Increases Proliferation and Cisplatin Sensitivity of Osteosarcoma-Derived Cells. PLoS ONE. Aug 12 2016 [PMID: 27513462] (WB)
• Leinonen H, Rossi M, Salo AM et al. Lack of P4H-TM in mice results in age-related retinal and renal alterations. Hum. Mol. Genet. Jul 27 2016 [PMID: 27466183] (WB, Mouse)
• Sato T, Ito Y, Nagasawa T. Regulatory effects of the L-lysine metabolites, L-2-aminoadipic acid and L-pipecolic acid, on protein turnover in C2C12 myotubes Biosci. Biotechnol. Biochem. 2016 Jul 18 [PMID: 27427787]
• Wong AY, Hristova E, Ahlskog N et al. ABERRANT SUBCELLULAR DYNAMICS OF SIGMA-1 RECEPTOR MUTANTS UNDERLYING NEUROMUSCULAR DISEASES Mol. Pharmacol. 2016 Jul 14 [PMID: 27418673] (WB, Human)
• Ekser B, Mangus RS, Kubal CA et al. Recurrence of Hyperoxaluria and Kidney Disease after Combined Intestine-Kidney Transplantation for Enteric Hyperoxaluria Am. J. Nephrol. 2016 Jul 14 [PMID: 27410399] (WB, Mouse)
• Lee MC, Huang HJ, Chang TH et al. Genome-wide analysis of HIF-2alpha chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions. Sci Rep 2016 Jul 04 [PMID: 27373565] (WB)
• Xu Y, Li F, Zalzala M et al. Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice Hepatology 2016 Jun 30 [PMID: 27359351] (WB)
• Goettsch C, Hutcheson JD, Hagita S et al. A single injection of gain-of-function mutant PCSK9 adeno-associated virus vector induces cardiovascular calcification in mice with no genetic modification. Atherosclerosis Jun 9 2016 [PMID: 27318830] (WB, Mouse)
• Tsai WL, Yeh PH, Tsai CY et al. Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. May 24 2016 [PMID: 27218433] (WB, Human)
  
  Details:
  beta-Actin antibody used as a loading control for WB on human samples (protein lysates from differentiating cells – hLiver, Huh7.5.1 and HepG2 cells) (Fig. 2C).
• Bhoopathi P, Lee N, Pradhan AK et al. mda-7/IL-24 induces cell death in neuroblastoma through a novel mechanism involving AIF and ATM. Cancer Research. 2016 Apr 13 [PMID: 27197168] (WB, Human)
  
  Details:
  Beta actin antibody was used for Western blot application in experiments involving human neuroblastoma cancer cell lines namely SK-N-AS, SK-NSH and NB1691.
• Pastore N, Brady OA, Diab HI et al. TFEB and TFE3 Cooperate in the Regulation of the Innate Immune Response in Activated Macrophages. Autophagy. May 12 2016 [PMID: 27171064] (WB, Mouse)
• Radulovic M, Yoon H, Wu J et al. Targeting the thrombin receptor modulates inflammation and astrogliosis to improve recovery after spinal cord injury. Neurobiol. Dis. May 1 2016 [PMID: 27145117] (WB, Mouse)
• Boothe T, Lim GE, Cen H et al. Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells. Mol Metab 2016 May [PMID: 27110488] (WB)
• Lai CW, Chen HL, Tsai TC et al. Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling. Sci Rep. 2016 Apr 18 [PMID: 27087367] (WB, Mouse)
• Xu J, Xu Y, Li Y et al. Carboxylesterase 1 Is Regulated by Hepatocyte Nuclear Factor 4alpha and Protects Against Alcohol- and MCD diet-induced Liver Injury. Sci Rep. 2016 Apr 14 [PMID: 27075303] (WB, Mouse)
• Acharya M, Sokolovska A, Tam JM et al. alphav Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells. Nat Commun. 2016 Mar 11 [PMID: 26965188] (WB, Mouse)
• Naik E, Dixit VM. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCbeta Kinase Activity. J. Immunol. 2016 Mar 02 [PMID: 26936881] (WB, Mouse)
  
  Details:
  LDLR antibody was used for WB detection of Low-density lipoprotein receptorin lysates from liver tissues of Seipin?/? and wild type mice (Fig. 1E). LDLR was found to be highly unregulated in Seipin?/? mice compated to WT mice.
• Hong MN, Nam KY, Kim KK et al. The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha. Cell Stress Chaperones 2016 May [PMID: 26873011]
• Andreev E, Brosseau N, Carmona E et al. The human organic cation transporter OCT1 mediates high affinity uptake of the anticancer drug daunorubicin Sci Rep Feb 10 2016 [PMID: 26861753] (WB, Human)
• Gosejacob D, Jager PS, Vom Dorp K et al. Ceramide Synthase 5 Is Essential to Maintain C16:0-Ceramide Pools and Contributes to the Development of Diet-induced Obesity. J. Biol. Chem. Mar 25 2016 [PMID: 26853464] (WB, ELISA, Mouse)
• Yoon H, Kleven A, Paulsen A et al. Interplay between exercise and dietary fat modulates myelinogenesis in the central nervous system Biochim. Biophys. Acta Jan 26 2016 [PMID: 26826016] (WB, Mouse)
• Maj M, Hoermann G, Rasul S et al. The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells. J Diabetes Res 2016 [PMID: 26824039] (WB)
• Westwell-Roper C, Denroche HC, Ehses JA, Verchere CB et al. Differential activation of innate immune pathways by distinct islet amyloid polypeptide (IAPP) aggregates J. Biol. Chem. Jan 19 2016 [PMID: 26786104] (WB, Mouse)
• Bohnsack JP, Carlson SL, Morrow AL. Differential regulation of synaptic and extrasynaptic alpha4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology 2016 Jun [PMID: 26767953]
• Davis SM, Collier LA, Leonardo CC et al. Leukemia Inhibitory Factor Protects Neurons from Ischemic Damage via Upregulation of Superoxide Dismutase 3. Mol. Neurobiol. 2016 Jan 09 [PMID: 26746670] (WB, Rat)
• Lee SB, Frattini V, Bansal M et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016 Jan 14 [PMID: 26735018] (WB, Human)
• Cerqueira FM, Chausse B, Baranovski BM et al. Diluted Sera From Calorie Restricted Animals Promote Mitochondrial Beta-Cell Adaptations and Protect Against Glucolipotoxicity. FEBS J. 2016 Jan 06 [PMID: 26732506] (WB, Rat)
  
  Details:
  Beta-Actin antibody used for WB as a loading control on serum from AL and CR rats at a dilution of 1:2000 (Figure 3A).
• Lee KC, Hsieh YC, Yang YY et al. Aliskiren Reduces Hepatic steatosis and Epididymal Fat Mass and Increases Skeletal Muscle Insulin Sensitivity in High-Fat Diet-Fed Mice. Sci Rep 2016 Jan 06 [PMID: 26732252] (WB)
• Kral R, Doriguzzi A, Mayer CE et al. Differential Effects of Variations at Codon 106 on Sprouty2 Functions in Lung Cancer-Derived Cells. J. Cell. Biochem. 2016 Jan 05 [PMID: 26727965] (WB, Human)
  
  Details:
  Beta-Actin antibody used as a loading control for WB on WI-38, A549 and VL-8 cells that were infected with adenoviruses (Figure 1A).
• Yu CY, Liang JJ, Li JK et al. Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins. PLoS Pathog. 2015 Dec 01 [PMID: 26717518] (WB, Human)
• Wang TK, Lin YM, Lo CM et al. Oncogenic roles of carbonic anhydrase 8 in human osteosarcoma cells. Tumour Biol. 2015 Dec 28 [PMID: 26711783] (WB, Human)
  
  Details:
  beta-Actin antibody (clone AC-15) was used for WB assays in experiments involving human osteosarcoma cell lines MG-63, U-2 OS, HOS and 143B and human embryonic kidney cell line HEK293.
• Laukka T, Mariani CJ, Ihantola T et al. Fumarate and Succinate Regulate Expression of Hypoxia-inducible Genes via TET Enzymes. J Biol Chem 2016 Feb 19 [PMID: 26703470]
• Vannuvel K, Van Steenbrugge M, Demazy C et al. Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population. J. Cell. Physiol. 2015 Dec 17 [PMID: 26680008] (WB, Human)
• Sorrentino NC, Maffia V, Strollo S et al. A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs. Mol. Ther. 2015 Dec 07 [PMID: 26639405] (WB, Porcine)
• Suriben R, Kaihara KA, Paolino M et al. beta-Cell Insulin Secretion Requires the Ubiquitin Ligase COP1. Cell. 2015 Dec 03 [PMID: 26627735] (WB, Mouse)
• Yang Y, Jin Y, Du W. Programmed cell death 2 functions as a tumor suppressor in osteosarcoma. Int J Clin Exp Pathol. 2015 Nov 30 [PMID: 26617804] (WB, Rat)
• Cheng YF, Young GH, Lin JT et al. Activation of AMP-Activated Protein Kinase by Adenine Alleviates TNF-Alpha-Induced Inflammation in Human Umbilical Vein Endothelial Cells. PLoS ONE. 2015 Nov 07 [PMID: 26544976] (WB, Human)
• Lin CL, Cheng YS, Li HH et al. Amyloid-beta suppresses AMP-activated protein kinase (AMPK) signaling and contributes to alpha-synuclein-induced cytotoxicity. Exp Neurol 2016 Jan [PMID: 26515689]
• Lin CW, Chen YS, Lin CC et al. Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy. Sci Rep. 2015 Oct 30 [PMID: 26515640] (WB, Mouse)
• Weng WH, Leung WH, Pang YJ, Hsu HH Lauric acid can improve the sensitization of Cetuximab in KRAS/BRAF mutated colorectal cancer cells by retrievable microRNA-378 expression. Oncol Rep 2016 Jan [PMID: 26496897]
• Karsikas S, Myllymaki M, Heikkila M et al. HIF-P4H-2 deficiency protects against skeletal muscle ischemia-reperfusion injury. J Mol Med (Berl) 2016 Mar [PMID: 26452676]
• Shi C, Huang X, Zhang B et al. The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein. PLoS One 2015 [PMID: 26414348] (ChIP)
• Qiu Q, Yang C, Xiong W et al. SYK is a target of lymphocyte-derived microparticles in the induction of apoptosis of human retinoblastoma cells. Apoptosis 2015 Dec [PMID: 26404525] (Human)
• Koukourakis MI, Kalamida D, Giatromanolaki A et al. Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. PLoS ONE 2015 Sep 18 [PMID: 26378792] (WB, Human)
• Sato T, Ito Y, Nagasawa T. Dietary l-Lysine Suppresses Autophagic Proteolysis and Stimulates Akt/mTOR Signaling in the Skeletal Muscle of Rats Fed a Low-Protein Diet. J. Agric. Food Chem. 2015 Sep 23 [PMID: 26366928] (WB, Rat)
• Wu L, Zhang Z, Pan X, Wang Z. Expression and contribution of the HIF1a/VEGF signaling pathway to luteal development and function in pregnant rats Mol Med Rep 2015 Aug 28 [PMID: 26323652] (Rat)
  
  Details:
  This citation used the HRP version of this antibody.
• Radulovic M, Yoon H, Wu J et al. Genetic Targeting of Protease Activated Receptor 2 Reduces Inflammatory Astrogliosis and Improves Recovery of Function after Spinal Cord Injury. Neurobiol. Dis. 2015 Aug 24 [PMID: 26316358] (WB, Mouse)
• Sato T, Ito Y, Nagasawa T. Attenuation of autophagic-proteolysis in C2C12 cells by saccharopine. Mol. Cell. Biochem. 2015 Aug 26 [PMID: 26307368] (WB, Mouse)
• Lin CL, Huang WN, Li HH et al. Hydrogen-rich water attenuates amyloid beta-induced cytotoxicity through upregulation of Sirt1-FoxO3a by stimulation of AMP-activated protein kinase in SK-N-MC cells. Chem. Biol. Interact. 2015 Aug 10 [PMID: 26271894] (WB, Human)
  
  Details:
  beta-Actin antibody was used for WB analysis of lysates from human neuroblastoma SK-N-MC cells that were subjected to treatments with Amyloid beta and / or hydrogen-rich water in vitro.
• Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015 Aug 03 [PMID: 26230691] (WB, Chicken)
  
  Details:
  beta-Actin antibody (clone AC-15) was used at 1:10,000 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
• Lim GE, Albrecht T, Piske M et al. 14-3-3zeta coordinates adipogenesis of visceral fat. Nat Commun 2015 [PMID: 26220403] (IP)
• Bamidele AO, Kremer KN, Hirsova P et al. IQGAP1 promotes CXCR4 chemokine receptor function and trafficking via EEA-1+ endosomes J. Cell Biol. 2015 Jul 20 [PMID: 26195666] (WB, Human)
• Chia-Wei Lin, Ming-Shiou Jan, Jung-Hua Steven Kuo. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. 2015 Aug 3 [PMID: 26158199]
• Yang C, Fischer-Keso R, Schlechter T et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression Tumour Biol. 2015 Jul 04 [PMID: 26138584] (WB, Human)
• Sasaki Y, Ohta M, Desai D et al. Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance PLoS ONE. 2015 Jul 03 [PMID: 26132105] (WB, Human, Mouse)
• Yang JS, Lin CW, Chuang CY et al. Carbonic anhydrase IX overexpression regulates the migration and progression in oral squamous cell carcinoma Tumour Biol. 2015 Jul 01 [PMID: 26130414] (WB, Human)
• Smith BW, Simpson DG, Miller RJ et al. Contrast Ultrasound Imaging Does Not Affect Heat Shock Protein 70 Expression in Cholesterol-Fed Rabbit Aorta J Ultrasound Med. 2015 Jul 01 [PMID: 26112623] (WB, Rabbit)
• Miki Y, Akimoto J, Moritake K et al. Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells Lasers Med Sci. 2015 Jun 25 [PMID: 26109138] (WB, Human)
• Chang CJ, Lin CS, Lu CC et al. Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun 2015 [PMID: 26102296] (Mouse)
• Xu Y, Zalzala M, Xu J et al. A metabolic stress-inducible miR-34a-HNF4a pathway regulates lipid and lipoprotein metabolism Nat Commun 2015 Jun 23 [PMID: 26100857] (WB, Mouse)
• Billeter AT, Galbraith N, Walker S et al. TRPA1 mediates the effects of hypothermia on the monocyte inflammatory response Surgery. 2015 Jun 05 [PMID: 26054320] (WB, Human)
• Viale-Bouroncle S, Klingelhoffer C, Ettl T, Morsczeck C. The AKT signaling pathway sustains the osteogenic differentiation in human dental follicle cells. Mol. Cell. Biochem. 2015 May 16 [PMID: 25981531] (WB, Human)
• Zhang Z, Pang X, Tang Z et al. Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015 Sep [PMID: 25975603]
  
  Details:
  This publication used the HRP conjugated form of this antibody (NB600-501H)
• Li HH, Lu FJ, Hung HC et al. Humic Acid Increases Amyloid beta-Induced Cytotoxicity by Induction of ER Stress in Human SK-N-MC Neuronal Cells.. Int J Mol Sci. 2015 May 7 [PMID: 25961951]
• Doriguzzi A, Haigl B, Gsur A, Sutterluty-Fall H. The increased Sprouty4 expression in response to serum is transcriptionally controlled by Specific protein 1. Int. J. Biochem. Cell Biol. 2015 May 06 [PMID: 25957915] (WB, Human)
• Wang ML, Lin SH, Hou YY, Chen YH. alpha-Naphthoflavone Increases Lipid Accumulation in Mature Adipocytes and Enhances Adipocyte-Stimulated Endothelial Tube Formation. Nutrients. 2015 May 06 [PMID: 25942489] (WB, Human)
• Yen JH, Lin LC, Chen MC, Sarang Z. The metastatic tumor antigen 1-transglutaminase-2 pathway is involved in selflimitationof monosodiumurate crystal-induced inflammation byupregulating TGF-beta1 Arthritis Research & Therapy 2015 Feb 26 [PMID: 25889736] (WB, Mouse, Human)
• Kao YT, Chang BL, Liang JJ et al. Japanese Encephalitis Virus Nonstructural Protein NS5 Interacts with Mitochondrial Trifunctional Protein and Impairs Fatty Acid beta-Oxidation PLoS Pathog. 2015 Mar 01 [PMID: 25816318] (WB, Human)
• Bonora M, Wieckowsk MR, Chinopoulos C et al. Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition. Oncogene 2015 Mar 19 [PMID: 25790189]
• Matteucci C, Minutolo A, Marino-Merlo F et al. Characterization of the enhanced apoptotic response to azidothymidine by pharmacological inhibition of NF-kB Life Sci. 2015 Mar 02 [PMID: 25744407] (WB, Human)
  
  Details:
  Beta-actin antibody was used as loading control for WB application in exeperiments involving AZT-induced NF-kB activation and its suppression by combined treatment with Bay-11-7085 in U937 cells (Fig. 2A).
• Wen J, Hanna CW, Martell S et al. Functional consequences of copy number variants in miscarriage Mol Cytogenet. 2015 Feb 12 [PMID: 25674159] (WB, Human)
• Capo-Chichi JM, Boissel S, Brustein E et al. Disruption of CLPB is associated with congenital microcephaly, severe encephalopathy and 3-methylglutaconic aciduria J. Med. Genet. 2015 Feb 03 [PMID: 25650066] (WB, Human, Zebrafish)
• Yoon H, Radulovic M, Drucker KL et al. The thrombin receptor is a critical extracellular switch controlling myelination Glia. 2015 Jan 27 [PMID: 25628003] (WB, Mouse)
• Chong KY, Hsu CJ, Hung TH et al. Wnt pathway activation and ABCB1 expression account for attenuation of Proteasome Inhibitor-mediated apoptosis in multidrug-resistant cancer cells Cancer Biol. Ther. 2015 Jan 15 [PMID: 25590413] (WB)
• Peng CC, Lin YT, Chen KC et al. Antrodan, A beta-glucan obtained from Antrodia cinnamomea mycelia, is beneficial to benign prostate hyperplasia.. Food Funct. 2015 Feb 11 [PMID: 25537160]
• Taher Chato, Frisk Gabriella, Fuentes Stina et al. High prevalence of human cytomegalovirus in brain metastases of patients with primary breast and colorectal cancers. Transl Oncol. 2014 Dec 01 [PMID: 25500083]
• Lin Sp, Chiu Fy, Wang Y et al. RB Maintains Quiescence and Prevents Premature Senescence through Upregulation of DnMT1 in Mesenchymal Stromal Cells. Stem Cell Reports. 2014 Nov 06 [PMID: 25455074] (WB, Human)
• Hsin Ch, Chen Mk, Tang Ch et al. High level of plasma matrix metalloproteinase-11 is associated with clinicopathological characteristics in patients with oral squamous cell carcinoma. PLoS OnE. 2014 Nov 26 [PMID: 25423087] (WB, Human)
• Pan Wr, Chen Yl, Hsu Hc,Chen Wj. Antimicrobial peptide GW-H1-induced apoptosis of human gastric cancer AGS cell line is enhanced by suppression of autophagy. Mol. Cell. Biochem. 2014 Nov 08 [PMID: 25380626] (WB, Human)
• Liu Y, Shim E, Crespo-Mejias Y et al. Cardiomyocytes are Protected from Antiretroviral nucleoside Analog-Induced Mitochondrial Toxicity by Overexpression of PGC-1Alpha Cardiovasc. Toxicol. 2014 Nov 07 [PMID: 25377427] (WB, Rat)
  
  Details:
  Beta actin antibody used for WB in experiments involving H9c2 cell line (derived from embryonic rat heart tissue).
• Chan Mt, Lim Ge, Skovso S et al. Effects of insulin on human pancreatic cancer progression modeled in vitro BMC Cancer et al. 2014 Nov 14 [PMID: 25373319] (WB, Human)
  
  Details:
  Beta actin antibody used as loading control for WB in experiments involving human primary pancreatic ductal cells, human HPDE cells and human PAnC1 cells.
• Lajud Sa, Nagda Da, Yamashita T et al. Dual Disruption of DnA Repair and Telomere Maintenance for the Treatment of Head and neck Cancer. Clin. Cancer Res. 2014 Oct 16 [PMID: 25324139] (WB, Human)
• Kokontis JM, Lin HP, Jiang SS et al. Androgen Suppresses the Proliferation of Androgen Receptor-Positive Castration-Resistant Prostate Cancer Cells via Inhibition of Cdk2, CyclinA, and Skp2. PLoS ONE. 2014 Oct 02 [PMID: 25271736] (WB, Human)
• Fischer-Keso R, Breuninger S, Hofmann S et al. Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins. Mol Cell Biol. 2014 Sep 15 [PMID: 25225333]
• Naik E, Webster JD, DeVoss J et al. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp. Med. 2014 Sep 22 [PMID: 25200027] (WB, Mouse)
• Qin Q, Xie H, Wise SS et al. Homologous Recombination Repair Signaling in Chemical Carcinogenesis: Prolonged Particulate Hexavalent Chromium Exposure Suppresses the Rad51 Response in Human Lung Cells. Toxicol. Sci. 2014 Aug 30 [PMID: 25173789] (WB, Human)
• Yang NC, Song TY, Chang YZ et al. Up-regulation of nicotinamide phosphoribosyltransferase and increase of NAD+ levels by glucose restriction extend replicative lifespan of human fibroblast Hs68 cells.. Biogerontology. 2015 Feb [PMID: 25146190]
• Hung YW, Hung SW, Wu YC et al. Soluble epoxide hydrolase activity regulates inflammatory responses and seizure generation in two mouse models of temporal lobe epilepsy Brain Behav. Immun. 2014 Aug 15 [PMID: 25135858]
• Dill-McFarland KA, Neil KL, Zeng A et al. Hibernation alters the diversity and composition of mucosa-associated bacteria while enhancing antimicrobial defense in the gut of 13-lined ground squirrels. Mol. Ecol. 2014 Aug 12 [PMID: 25130694] (WB, Mammal)
• Di K, Keir ST, Alexandru-Abrams D et al. Profiling Hsp90 differential expression and the molecular effects of the Hsp90 inhibitor IPI-504 in high-grade glioma models J. Neurooncol. 2014 Aug 13 [PMID: 25115740]
• Benayoun BA, Pollina EA, Ucar D et al. H3K4me3 Breadth Is Linked to Cell Identity and Transcriptional Consistency Cell 2014 Jul 31 [PMID: 25083876]
• Lin CW, Jan MS, Kuo JH. Autophagy-related gene expression analysis of wild-type and atg5 gene knockout mouse embryonic fibroblast cells treated with polyethylenimine. Mol Pharm. 2014 Sep 2 [PMID: 25075440]
• Bohr M, Brooks AR, Kurtz CC. Hibernation induces immune changes in the lung of 13-lined ground squirrels (Ictidomys tridecemlineatus). Dev. Comp. Immunol. 2014 Jul 18 [PMID: 25046152] (WB, Squirrel)
  
  Details:
  Beta Actin antibody used as loading control for WB on lung of 13-lined ground squirrels (Ictidomys tridecemlineatus).
• Vande Walle Lieselotte, Van Opdenbosch Nina, Jacques Peggy et al. Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis. Nature 2014 Aug 07 [PMID: 25043000] (WB, Mouse)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Chen Chin-Chen, Lin Jiun-Tsai, Cheng Yi-Fang et al. Amelioration of LPS-induced inflammation response in microglia by AMPK activation. Biomed Res Int. 2014 Jun 17 [PMID: 25025067]
• Wu CM, Lin KW, Teng CH et al. Chalcone derivatives inhibit human platelet aggregation and inhibit growth in human bladder cancer cells. Biol. Pharm. Bull. 2014 Jul 03 [PMID: 24989010] (WB, Human)
  
  Details:
  Beta actin antibody used as a loading control in WB application in lysates of human bladder cancer cells NTUB1.
• Lin KW, Huang AM, Lin CC et al. Anti-cancer effects of ursane triterpenoid as a single agent and in combination with cisplatin in bladder cancer Eur. J. Pharmacol. 2014 Jun 14 [PMID: 24933647] (WB, Human)
  
  Details:
  Beta Actin antibody used as loading control in WB assays on NTUB1 (a human bladder carcinoma cell) treated or not with Ursolic acid derivative isopropyl 3beta-hydroxyurs-12-en-28-oat (UA17) and cisplatin combinations. WB Images - Fig. 3C, Fig. 10 B and C.
• Chung PJ, Chi LM, Chen CL et al. microRNA-205 targets tight junction-related proteins during urothelial cellular differentiation. Mol. Cell Proteomics. 2014 Jun 09 [PMID: 24912853] (WB, Mouse)
  
  Details:
  Beta Actin antibody used for WB in 550ug lysate of normal urothelial cells (isolated from mouse bladder) treated with the antogomir against miR-205 (antagomiR-205 or Ant-205) or scramble control.
• Rahtu-Korpela Lea, Karsikas Sara, Horkko Sohvi et al. HIF prolyl 4-hydroxylase-2 inhibition improves glucose and lipid metabolism and protects against obesity and metabolic dysfunction. Diabetes. 2014 May 01 [PMID: 24789921] (Mouse)
• Press T, Viale-Bouroncle S, Felthaus O et al. EGR1 supports the osteogenic differentiation of dental stem cells. Int Endod J 2014 Apr 21 [PMID: 24749562] (WB, Human)
• Zeng W, Guo YH, Qi W et al. 4-Phenylbutyric acid suppresses inflammation through regulation of endoplasmic reticulum stress of endothelial cells stimulated by uremic serum. Life Sci. 3/17/2014 [PMID: 24650493] (WB, Human)
• Brugger D, Buffler M, Windisch W. Development of an experimental model to assess the bioavailability of zinc in practical piglet diets. Arch Anim Nutr 2014 Apr 1 [PMID: 24646148] (WB, Porcine)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Wong TW, Doyle AD, Lee JJ, Jelinek DF. Eosinophils Regulate Peripheral B Cell Numbers in Both Mice and Humans. J. Immunol. 3/10/2014 [PMID: 24616476] (WB, Human)
• Cai B, Xie S, Liu F et al. Rapid Degradation of the Complement Regulator, CD59, by a Novel Inhibitor. J. Biol. Chem. 3/10/2014 [PMID: 24616098] (WB, Human)
• Carmony KC. Elucidating Proteasome Catalytic Subunit Composition and Its Role in Proteasome Inhibitor Resistance. Thesis. Apr 18 2016 (WB, Human)
• Liu Y, Shim E, Nguyen P et al. Tempol Protects Cardiomyocytes from Nucleoside Reverse Transcriptase Inhibitor-Induced Mitochondrial Toxicity. Toxicol. Sci. 3/10/2014 [PMID: 24591154] (WB, Rat)
• Wu L, Zhang Z, Pan X, Wang Z. Expression and contribution of the HIF1a/VEGF signaling pathway to luteal development and function in pregnant rats Mol Med Rep (Rat)
  
  Details:
  This publication used the HRP conjugated form of this antibody (Cat# NB600-501H).
• Hung TM, Ho CM, Liu YC et al. Up-Regulation of MicroRNA-190b Plays a Role for Decreased IGF-1 That Induces Insulin Resistance in Human Hepatocellular Carcinoma. PLoS ONE 2014 Mar 3 [PMID: 24586785] (WB, Human)
• Zheng Xiaoxu, Heaps Cristine L, Fisher Steven A. Myosin phosphatase isoforms and related transcripts in the pig coronary circulation and effects of exercise and chronic occlusion. Microvasc Res. 2014 [PMID: 24534069] (Bovine)
• Van Opdenbosch Nina, Gurung Prajwal, Vande Walle Lieselotte et al. Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation. Nat Commun 2014 [PMID: 24492532] (WB)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Liang JJ, Wei JC, Lee YL et al. Surfactant-modified nanoclay exhibits an antiviral activity with high potency and broad spectrum. J. Virol. 2014 Jan 30 [PMID: 24478435] (WB, Human, Hamster)
• Yu CH, Yu CC. Photodynamic therapy with 5-aminolevulinic acid (ALA) impairs tumor initiating and chemo-resistance property in head and neck cancer-derived cancer stem cells. PLoS ONE 2014 Jan 29 [PMID: 24475244] (WB, Human)
• D'souza AM, Asadi A, Johnson JD, Kieffer TJ. Leptin Deficiency in Rats Results in Hyperinsulinemia and Impaired Glucose Homeostasis. Endocrinology 2014 Jan 28 [PMID: 24467741] (WB, Rat)
• Li KW, Yu YP, Zhou C et al. Calcium channel alpha-2-delta1 proteins mediate trigeminal neuropathic pain states associated with aberrant excitatory synaptogenesis. J. Biol. Chem. 2014 Jan 24 [PMID: 24459143] (WB, Rat)
• Lin HP, Lin CY, Hsiao PH et al. Difference in Protein Expression Profile and Chemotherapy Drugs Response of Different Progression Stages of LNCaP Sublines and Other Human Prostate Cancer Cells. PLoS One 2013 Dec 5 [PMID: 24349321] (WB, Human)
• Zhu M, Guo M, Fei L et al. 4-Phenylbutyric acid attenuates endoplasmic reticulum stress-mediated pancreatic beta-cell apoptosis in rats with streptozotocin-induced diabetes. Endocrine 2013 Dec 18 [PMID: 24347242] (WB, Rat)
• Mounce BC, Mboko WP, Kanack AJ et al. Primary macrophages rely on histone deacetylase 1 and 2 expression to induce type I interferon in response to gammaherpesvirus infection. J. Virol. 2014 Feb 1 [PMID: 24335310] (WB, Mouse)
• Boothe T, Lim GE, Cen H et al. Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells Molecular Metabolism Feb 10 2016 (WB, Mouse)
• Kaczmarek MZ, Holland RJ, Lavanier SA et al. Mechanism of action for the cytotoxic effects of the nitric oxide prodrug JS-K in murine erythroleukemia cells. Leuk Res 2013 Dec 12 [PMID: 24461365] (WB, Mouse)
• Viale-Bouroncle S, Gosau M, Morsczeck C. NOTCH1 signaling regulates the BMP2/DLX-3 directed osteogenic differentiation of dental follicle cells. Biochem Biophys Res Commun 2014 Jan 10 [PMID: 24321094] (WB, Human)
• Schusser B, Yi H, Collarini EJ et al. Harnessing Gene Conversion in Chicken B Cells to Create a Human Antibody Sequence Repertoire. PLoS One. 2013 Nov 21 [PMID: 24278246] (WB, Chicken)
• Govaert KM, Emmink BL, Nijkamp MW et al. Hypoxia After Liver Surgery Imposes an Aggressive Cancer Stem Cell Phenotype on Residual Tumor Cells. Ann Surg 2013 Nov 18 [PMID: 24253142] (WB, Mouse)
• Allache R, Lachance S, Guyot MC et al. Novel mutations in Lrp6 orthologs in mouse and human neural tube defects affect a highly dosage-sensitive Wnt non-canonical planar cell polarity pathway. Hum Mol Genet. 2013 Nov 18 [PMID: 24203697] (WB, Human)
• Zhou C, Luo ZD. Electrophysiological characterization of spinal neuron sensitization by elevated calcium channel alpha-2-delta-1 subunit protein. Eur J Pain. 2013 Oct 23 [PMID: 24151064]
• Thies HW, Nolte I, Wenk H et al. Permanent activation of HMGA2 in lipomas mimics its temporal physiological activation linked to the gain of adipose tissue. Obesity (Silver Spring). 2012 Nov 6 [PMID: 24106133] (WB, Canine, Human)
• Xu J, Li Y, Chen WD et al. Hepatic carboxylesterase 1 Is essential for both normal and farnesoid X receptor-controlled lipid homeostasis. Hepatology. 2013 Aug 28 [PMID: 24038130] (WB, Mouse)
• Gini B, Zanca C, Guo D et al. The mTOR kinase inhibitors, CC214-1 and CC214-2, preferentially block the growth of EGFRvIII-activated glioblastomas. Clin Cancer Res 2013 Oct 15 [PMID: 24030701]
• Li KW, Kim DS, Zaucke F et al. Trigeminal nerve injury-induced thrombospondin-4 up-regulation contributes to orofacial neuropathic pain states in a rat model. Eur J Pain. 2013 Sep 9 [PMID: 24019258]
• Snoeren N, Emmink BL, Koerkamp MJ et al. Maspin is a marker for early recurrence in primary stage III and IV colorectal cancer. Br J Cancer. 2013 Sep 17 [PMID: 24002600] (WB, Human)
• Feldman AL, Vasmatzis G, Asmann YW et al. Novel TRAF1-ALK fusion identified by deep RNA sequencing of anaplastic large cell lymphoma. Genes Chromosomes Cancer. 2013 Sep 2 [PMID: 23999969] (WB, Human)
• Chan CC, Lee KC, Huang YH et al. Regulation by Resveratrol of the Cellular Factors Mediating Liver Damage and Regeneration after Acute Toxic Liver Injury. J Gastroenterol Hepatol. 2013 Aug 23 [PMID: 23981054] (WB, Mouse)
• Liu L, Martin R, Kohler G, Chan C. Palmitate induces transcriptional regulation of BACE1 and presenilin by STAT3 in neurons mediated by astrocytes. Exp Neurol 2013 Oct [PMID: 23968646] (WB, Rat)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Chang KP, Wang CL, Kao HK et al. Overexpression of caldesmon is associated with lymph node metastasis and poorer prognosis in patients with oral cavity squamous cell carcinoma. Cancer. 2013 Aug 20 [PMID: 23963810] (WB, Human)
• Chia-Feng Kuo, Chih-Hsuan Hsieh, Wun-Yuan Lin. Proteomic response of LAP-activated RAW 264.7 macrophages to the anti-inflammatory property of fungal ergosterol.. Food chemistry. 2011
• Khan Z, Yaiw KC, Wilhelmi V et al. Human cytomegalovirus immediate early proteins promote degradation of connexin 43 and disrupt gap junction communication: implications for a role in gliomagenesis. Carcinogenesis 2014 Jan [PMID: 23978378]
• Hsu PK, Chen HY, Yeh YC et al. TPX2 expression is associated with cell proliferation and patient outcome in esophageal squamous cell carcinoma. J Gastroenterol. 2013 Aug 21 [PMID: 23963785] (WB, Human)
• Lee CC, Lai YT, Chang HT et al. Inhibition of high-mobility group box 1 in lung reduced airway inflammation and remodeling in a mouse model of chronic asthma. Biochem Pharmacol. 2013 Aug 12 [PMID: 23948063] (WB, Mouse)
• Yeh CN, Chiang KC, Juang HH et al. Reappraisal of the Therapeutic Role of Celecoxib in Cholangiocarcinoma. PLoS One 2013 Jul 26 [PMID: 23922859] (WB, Rat)
• Hsieh YT, Chou MM, Chen HC, Tseng JJ. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. Gynecol Oncol. 2013 Oct [PMID: 23911878]
• Morel E, Chamoun Z, Lasiecka ZM et al. Phosphatidylinositol-3-phosphate regulates sorting and processing of amyloid precursor protein through the endosomal system. Nat Commun 2013 Aug 2 [PMID: 23907271] (WB, Human)
• Tigrine C, Bulzomi P, Leone S et al. Cleome arabica leaf extract has anticancer properties in Human cancer cells. Pharm Biol 2013 Jul 18 [PMID: 23862683] (WB, Human)
• Wen J, Lopes F, Soares G et al. Phenotypic and functional consequences of haploinsufficiency of genes from exocyst and retinoic acid pathway due to a recurrent microdeletion of 2p13.2. Orphanet J Rare Dis 2013 Jul 10 [PMID: 23837398] (WB, Human)
• Emmink BL, Verheem A, Van Houdt WJ et al. The secretome of colon cancer stem cells contains drug-metabolizing enzymes. J Proteomics 2013 Jul 5 [PMID: 23835434] (WB, Human)
• Rathmanner N, Haigl B, Vanas V et al. Sprouty2 but not Sprouty4 is a potent inhibitor of cell proliferation and migration of osteosarcoma cells. FEBS Lett 2013 Jul 4 [PMID: 23831057] (WB, Human)
• Kremer KN, Peterson KL, Schneider PA et al. SDF-1/CXCR4 signaling induces apoptosis in acute myeloid leukemia cells via regulation of the Bcl-2 family members Bcl-XL, Noxa, and Bak. J Biol Chem 2013 Jun 24 [PMID: 23798675] (WB, Human)
• Ko CH, Cheng CF, Lai CP et al. Differential proteomic analysis of cancer stem cell properties in hepatocellular carcinomas by isobaric tag labeling and mass spectrometry. J Proteome Res 2013 Aug 2 [PMID: 23782096] (WB, Human)
• Shie MY, Ding SJ. Integrin binding and MAPK signal pathways in primary cell responses to surface chemistry of calcium silicate cements. Biomaterials 2013 Sep [PMID: 23768900] (ELISA, Human)
• Liu T, Zhang S, Xiang D, Wang Y. Induction of hepatocyte-like cells from mouse embryonic stem cells by lentivirus-mediated constitutive expression of Foxa2/Hnf4a. J Cell Biochem 2013 Jun 6 [PMID: 23744720] (WB, Mouse)
• Babic I, Anderson ES, Tanaka K et al. EGFR Mutation-Induced Alternative Splicing of Max Contributes to Growth of Glycolytic Tumors in Brain Cancer. Cell Metab 2013 Jun 4 [PMID: 23707073] (WB, Human)
• Formosa R, Xuereb-Anastasi A, Vassallo J. AIP regulates cAMP signalling and growth hormone secretion in GH3 cells. Endocr Relat Cancer 2013 May 23 [PMID: 23702468] (WB, ICC/IF, Rat)
• Das I, Png CW, Oancea I et al. Glucocorticoids alleviate intestinal ER stress by enhancing protein folding and degradation of misfolded proteins. J Exp Med 2013 May 6 [PMID: 23650437] (WB, Human)
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Reviews for beta-Actin Antibody (NB600-501) (22)

Average Rating: 4.8

(Based on 22 reviews)

We have 22 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Luiz Alexandre Magno WB Mouse 07/17/2018

Summary

• Application: Western Blot
• Sample Tested: Mouse brain,Brain (corpus striatum)
• Species: Mouse
• Lot: 014M4759

Comments

• Comments: I have used this antibody for several years and it has worked perfectly in our western blots. It works very well at 1:10,00 dilution when loading gels with 30 ug of protein extracts. I congratulate Novus Biologicals for this achievement.

reviewed by: Anonymous WB Human 02/20/2018

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human
• Lot: 105M4825V-3

reviewed by: Liyong Zhang WB Human 11/25/2017

Summary

• Application: Western Blot
• Sample Tested: mouse hepatocytes and HepG2 cells
• Species: Human
• Lot: 105M4825V-3

reviewed by: Anonymous WB Human 06/05/2015

Summary

• Application: Western Blot
• Sample Tested: human glioma cells
• Species: Human

Blocking

• Blocking Details: 10% milk in TBST

Details

• Detection Notes: ECL western blotting detection reagent

reviewed by: Anonymous WB Human 05/29/2015

Summary

• Application: Western Blot
• Sample Tested: Fibroblast Hs68 cell lysate
• Species: Human

Blocking

• Blocking Details: See PMID: 25146190

Details

• Detection Notes: See PMID: 25146190

Comments

• Comments: Effects of glucose restriction (GR) on the expression of Nampt protein in Hs68 cells. beta-Actin used as a loading control. See PMID: 25146190 for details

reviewed by: Anonymous WB Human 03/25/2015

Summary

• Application: Western Blot
• Sample Tested: Caco-2, T84, HCT116, HT29, DU145, BPH1, Hela
• Species: Human

Blocking

• Blocking Details: 1 hour at RT in 5% milk powder solution (in 1xTBST)

Primary Anitbody

• Dilution Ratio: Dilution:1/15000, 1 hour at RT

Secondary Antibody

• Secondary Description: HRP

Details

• Detection Notes: ECL detection

reviewed by: Anonymous WB Other 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: Whole Skeletal Muscle Lysate
• Species: Other

Blocking

• Blocking Details: 3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

• Dilution Ratio: 1/4000 at 4C for 18 hr

Secondary Antibody

• Secondary Description: Goat anti-Mouse 680 RD - Licor Biosciences
• Secondary Manufacturer Cat#: 925-68070
• Secondary Concentration: 1/20,000

Details

• Detection Notes: Licor Odyssey

reviewed by: Sandra Vieira WB Human 01/23/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates: CHO, GC-1, Hela, Cos-7, SH-SY5Y
• Species: Human
• Lot: 083M4851

Blocking

• Blocking Details: blocking solution was added for 1h (5% non-fat dry milk in 1x TBS-T)

Primary Anitbody

• Dilution Ratio: Primary antibody B-Actin (1:10000), diluted in 3% non-fat dry milk in 1x TBS-T, was added and incubated with membranes 1h

Secondary Antibody

• Secondary Description: Goat anti-mouse
• Secondary Concentration: 1:5000

Details

• Detection Notes: the enhanced chemiluminescence (ECL) detection method; Homemade ECL 1 min; Exposure time: 5 min

reviewed by: Liyong Zhang WB Mouse 07/01/2014

Summary

• Application: Western Blot
• Species: Mouse
• File: View PDF

reviewed by: Beatrice Gini WB Human 12/02/2013

Summary

• Application: Western Blot
• Sample Tested: human brain cells
• Species: Human
• Comments: Clin Cancer Res. 2013 Oct 15;19(20):5722-32.

Blocking

• Blocking Details: 5% BSA-TBS 1X-Tween 0.1% in dH2O, 1h, RT

Primary Anitbody

• Dilution Ratio: 1:10000, 1 h, RT, in TBS 1X-Tween 0.1% in dH2O

Secondary Antibody

• Secondary Description: anti-mouse, 1:5000

Details

• Detection Notes: Thermo detection reagent

Comments

• Comments: Clin Cancer Res. 2013 Oct 15;19(20):5722-32.

reviewed by: Anonymous WB Human 12/21/2011

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell line
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% FBS

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous WB Mouse 12/21/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse BALB/c macrophage cell line
• Species: Mouse
• Comments: The cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat milk in PBST, Blocking Time: 1 hour

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: Goat anti-Mouse IgG (HRP), Jackson

Details

• Detection Notes: Detection Method: GE ImageQuant LAS 4000 mini plus ECL

Comments

• Comments: The cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

reviewed by: Anonymous WB Mouse 12/14/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

Blocking

• Blocking Details: Blocking Buffer: 5% BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab Dilution Ratio: 1:10000

Details

• Detection Notes: Detection Method: HRP System, detect ECL signal by photographic film

reviewed by: Anonymous WB Human 12/08/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat milk

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse IgG HRP

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous WB Rat 12/06/2011

Summary

• Application: Western Blot
• Sample Tested: Rat
• Species: Rat

Blocking

• Blocking Details: Blocking Buffer: 5% Milk

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 1 hour

Secondary Antibody

• Secondary Description: Secondary Ab: Mouse

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous WB Human 12/05/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human
• Comments: Good!

Blocking

• Blocking Details: Blocking Buffer: 3% BSA, Blocking Time: 2 hours

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 16 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: Mouse Millipore, Secondary Ab Dilution Ratio: 1:10000

Details

• Detection Notes: Detection Method: ECL Detection

Comments

• Comments: Good!

reviewed by: Anonymous WB Mouse 11/30/2011

Summary

• Application: Western Blot
• Sample Tested: mouse brain
• Species: Mouse

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat dry milk in PBS

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: Anti-Mouse IgG HRP, Secondary Ab Dilution Ratio: 1:10000

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous WB Human 11/21/2011

Summary

• Application: Western Blot
• Sample Tested: Human Cell lines
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% TTBS

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous WB Other 11/09/2011

Summary

• Application: Western Blot
• Sample Tested: Chicken cell lines
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: Anti-mouse HRP

Details

• Detection Notes: Detection Method: ECL

reviewed by: Wei-I Shiao WB Mouse 09/02/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse Cells
• Species: Mouse

Blocking

• Blocking Details: Blocking Buffer: HyBlock blocking buffer from HyCell Biotec, Blocking Time: 1 minute

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 10 minutes

Secondary Antibody

• Secondary Description: Secondary Ab: Mouse IgG

Details

• Detection Notes: Detection Method: Viaglo HRP Chemilum Substrate Kit, Negative Control: Beta Actin

reviewed by: Courtney Kurtz WB Other 08/11/2011

Summary

• Application: Western Blot
• Sample Tested: Ground squirrel small intestinal lysate, Sample Amount: 50 ug
• Species: Other
• Comments: Antibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!

Blocking

• Blocking Details: Blocking Buffer: 4% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Incubation Dilution Buffer: 4% milk, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

• Secondary Description: Secondary Ab: Goat anti-mouse poly-HRP, Secondary Ab Dilution Ratio: 1:1500

Details

• Detection Notes: Detection Method: Opti-4CN, Exposure Time: < 10 s, Specific Bands: ~42 kDa

Comments

• Comments: Antibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!

reviewed by: WB Mouse 08/30/2010

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate, adult neural stem cell whole cell lysate
• Species: Mouse
• Comments: Very strong detection with 1:5000 dilution

Comments

• Comments: Very strong detection with 1:5000 dilution

FAQs for beta-Actin Antibody (NB600-501). (Showing 1 - 9 of 9 FAQs).

1. Why is beta actin used as a loading control in blotting ?
   • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
2. Are the beta-Actin antibodies validated in Simple Western?
   • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
3. Do Beta-Actin antibodies come in lyophilized format?
   • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
4. What the theoretical molecular weight for Beta-Actin antibodies?
   • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
5. We have these Abs: NB120-19481 PPAR gamma Antibody and NB600-501 Beta Actin Antibody (AC-15). The samples that we are analyzing are bovine ones. Please, confirm me if those proteins are valid like positive controls in WB for those antibodies: 1. Beta Actin Recombinant Protein (H00000060-P01). 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). Or perhaps could be better those lysates: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02 3. PPAR gamma Lysate - NBL1-14634 4. PPAR gamma Lysate - NBL1-14633 5. PPAR gamma Lysate - NBL1-14635.
   • I recommend the below controls as we have validated these as controls. NB120-19481: 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). NB600-501: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02
6. I would like to try the Beta Actin Antibody (AC-15) you have in stock to see if it reacts with fish samples in a Western Blot. Also, I would like to know if you have more information about its reactivity with fish?
   • We have tested that this antibody NB600-501 is reactive with fish. It has been tested with use in black carp. The homology with black carp is 99.0%.
7. Could anti-beta actin antibody recognize the actin derived from mosquito cells (C6/36)?
   • Unfortunately we have not used our antibody with catalogue number NB600-501 to detect beta actin that is derived from mosquito cells. You may however wish to align the peptide immunogen sequence against the full length mosquito beta actin sequence to check the homology. We usually suggest that a homology of greater than 80% is indicative of likely binding. The immunogen sequence for NB600-501 is Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (DDDIAALVIDNGSGK).
8. May we ask if NB600-501 has been tested with samples from zebrafish?
   • Beta Actin is a highly conserved protein and yes, our Beta Actin antibody (clone AC-15) NB600-501 is guaranteed for Zebrafish species.
9. What is the immunogen sequence of this Beta-Actin antibody?
   • A sequence (Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys) within the N-terminal region of beta Actin, conjugated to KLH.

Bioinformatics

• Gene Symbol: ACTB
• Entrez:
  • 60(Human)
  • 11461(Mouse)
  • 81822(Rat)