beta-Actin Antibody (AC-15)

Images

 
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Cell line lysates were separated on SDS-PAGE and probed with 1:5000 Monoclonal Anti-beta-Actin Clone: AC-15 . The antibody was developed using Goat Anti-Mouse ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin antibody on mouse fibroblasts. Image from verified customer review.
Immunohistochemistry: beta-Actin Antibody (AC-15) [NB600-501] - Staining of formalin-fixed, paraffin-embedded human heart sections with 1:1000 Monoclonal Anti-beta-Actin Clone: AC-15.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MMP-11 expression in oral cancer cell lines and the MMP-11 knockdown in SCC9 cells reduce cell migration. Western blot analysis showing the expression of MMP-11 ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HSP90 and HSP70 interact with PABPN1. Interaction of PABPN1 with HSP90. Lysates from HEK293 cells transfected with Flag-HSP90 and GFP-tagged A17-PABPN1 were ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - GFAP and IL-6 are elevated in cultures of spinal cord compared to cortical astrocytes. ((G, H) Analysis of protein homogenates from brain or spinal cord astrocyte ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - HS-68 cells.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 ...read more
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-)Species Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO
Clone
AC-15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta-Actin Antibody (AC-15) Summary

Immunogen
This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Epitope
N-terminus of the beta isoform of actin.
Specificity
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixing and paraffin-embedding.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
  • Simple Western 1:25
  • Western Blot 1:5000 - 1:10000
Application Notes
Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:25-1:100; matrix was 12-230 kDa.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 27 Reviews rated 4.8
using
NB600-501 in the following applications:

Publications
Read Publications using
NB600-501 in the following applications:

  • 1 publication
  • DB
    1 publication
  • 2 publications
  • 1 publication
  • 3 publications
  • IHC
    5 publications
  • 1 publication
  • 1 publication
  • IP
    2 publications
  • KD
    2 publications
  • KO
    1 publication
  • SW
    1 publication
  • WB
    361 publications

Reactivity Notes

The antibody cross reacts with beta-actin expressing cells in carp, leech tissues (Hirudo medicinalis), ground squirrel. Does not cross react with adult cardiac, skeletal muscle, drosophila or amoeba beta actin. Mammal reactivity and Hamster reactivity reported in scientific literature (PMID: 25130694 and 24478435 respectively). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Human reactivity reported in scientific literature (PMID:32938681) Use in Turkey reported in scientific literature (PMID:27816932).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for beta-Actin Antibody (AC-15)

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • anti-beta actin
  • B-Actin
  • beta actin (ac-15)
  • Beta Actin
  • beta cytoskeletal actin
  • beta-actin ac 15
  • beta-actin ac15
  • beta-actin ac-15
  • betaActin
  • beta-Actin
  • BRWS1
  • I(2)-Actin
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
NBP1-33527
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB500-317
Species: Bv(-), Ca(-), Ch(-), Hu, Mu(-), Rb(-)
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
NBP3-13174
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB100-93302
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-32710
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-30993
Species: Ha, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-67130
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB2669
Species: Hu
Applications: IHC, Simple Western, WB
NBP1-87511
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-33600
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, WB
NBP1-82859
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-130
Species: Bv, Ce, Ch, Dr, Eq, Hu, Pm, Mu, Pl, Po, Rt, Ze
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB
U-100H
Species: Hu
Applications: EnzAct
NBP2-94633
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
H00006124-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP2-75478
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-88192
Species: Hu
Applications: IHC, IHC-P, WB

Publications for beta-Actin Antibody (NB600-501)(510)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Bovine, C. elegans, Canine, Chicken, Drosophila melanogaster, Fish, Hamster, Mammal, Porcine, Rabbit, Squirrel, Turkey, Zebrafish.

We have publications tested in 13 applications: ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, SW, WB.


Filter By Application
ChIP
(1)
DB
(1)
ELISA
(2)
Flow
(1)
ICC/IF
(3)
IHC
(5)
IHC-Fr
(1)
IHC-P
(1)
IP
(2)
KD
(2)
KO
(1)
SW
(1)
WB
(361)
All Applications
Filter By Species
Human
(190)
Mouse
(124)
Rat
(44)
Bovine
(1)
C. elegans
(1)
Canine
(2)
Chicken
(3)
Drosophila melanogaster
(1)
Fish
(1)
Hamster
(2)
Mammal
(1)
Porcine
(4)
Rabbit
(5)
Squirrel
(1)
Turkey
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 510. Show All 510 Publications.
Publications using NB600-501 Applications Species
Zhao S, Li S, Yang J et al. GM-CSF-mediated inducement of bone marrow MDSCs by TSA and effect on survival of graft in mice European journal of medical research Aug 29 2022 [PMID: 36031660] (WB, Mouse) WB Mouse
Ollonen T, Kurkela M, Laitakari A et al. Activation of the hypoxia response protects mice from amyloid-beta accumulation Cellular and molecular life sciences : CMLS Jul 19 2022 [PMID: 35852609] (WB, Mouse)

Details:
1:5000 Dilution
WB Mouse
Zhu K, Sun S, Guo F, Gao L Impaired Fanconi anemia pathway causes DNA hypomethylation in human angiosarcomas Human cell Jul 11 2022 [PMID: 35817884] (WB) WB
Martin CE, Murray AS, Mackinder JR et al. TMPRSS13 zymogen activation, surface localization, and shedding is regulated by proteolytic cleavage within the non-catalytic stem region Biological chemistry Jul 8 2022 [PMID: 35796294]
Tapio J, Halmetoja R, Dimova E et al. Contribution of HIF-P4H isoenzyme inhibition to metabolism indicates major beneficial effects being conveyed by HIF-P4H-2 antagonism Journal of Biological Chemistry Jul 1 2022 [PMID: 35787374] (WB, Mouse) WB Mouse
Luchtel RA, Zhao Y, Aggarwal RK et al. ETS1 is a novel transcriptional regulator of adult T-cell leukemia/lymphoma of North American descent Blood advances Jun 8 2022 [PMID: 35675522] (WB, Human) WB Human
Patel S, Kumar S, Baldan S et al. Cytoplasmic proteotoxicity regulates HRI-dependent phosphorylation of eIF2alpha via the Hsp70-Bag3 module iScience Apr 1 2022 [PMID: 35573186] (WB, Human) WB Human
Esposito F, Lyubenova H, Tornabene P et al. Liver gene therapy with intein-mediated F8 trans-splicing corrects mouse haemophilia A EMBO molecular medicine May 2 2022 [PMID: 35491676] (WB, Human) WB Human
Herre C, Nshdejan A, Klopfleisch R et al. Expression of vimentin, TPI and MAT2A in human dermal microvascular endothelial cells during angiogenesis in vitro PloS one Apr 28 2022 [PMID: 35482724] (WB, Human) WB Human
Chen IC, Liu YC, Wu YH et al. Proteasome Inhibitors Decrease the Viability of Pulmonary Arterial Smooth Muscle Cells by Restoring Mitofusin-2 Expression under Hypoxic Conditions Biomedicines Apr 9 2022 [PMID: 35453623] (WB, Human) WB Human
Show All 510 Publications.

Reviews for beta-Actin Antibody (NB600-501) (27) 4.827

Average Rating: 4.8
(Based on 27 reviews)
We have 27 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB600-501:
Filter by Applications
ICC
(1)
WB
(26)
All Applications
Filter by Species
Human
(14)
Mouse
(8)
Rat
(2)
Other
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry beta-Actin NB600-501
Enlarge
5
reviewed by:
Lindsey Johnson
ICC Mouse 01/22/2022
View

Summary

ApplicationImmunocytochemistry
Sample Testedfibroblasts
SpeciesMouse
LotAC-15
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Tobias brun
WB Human 05/16/2021
View

Summary

ApplicationWestern Blot
Sample TestedHEK293
SpeciesHuman
Lot90942
  5
reviewed by:
Verified Customer
WB Human 01/31/2019
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 CELLS
SpeciesHuman
Lot106M4825V-4
  5
reviewed by:
Verified Customer
WB Rat 01/28/2019
View

Summary

ApplicationWestern Blot
Sample TestedMouse brain
SpeciesRat
Lot014M4759
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Arthur Von Houtt
WB Human 01/24/2019
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 07/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedBrain (corpus striatum),Mouse brain
SpeciesMouse
Lot014M4759

Comments

CommentsI have used this antibody for several years and it has worked perfectly in our western blots. It works very well at 1:10,00 dilution when loading gels with 30 ug of protein extracts. I congratulate Novus Biologicals for this achievement.
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Preeti Bharaj
WB Human 02/20/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Verified Customer
WB Human 11/25/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Kaijun Di
WB Human 06/05/2015
View

Summary

ApplicationWestern Blot
Sample Testedhuman glioma cells
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Anonymous -
WB Human 05/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedFibroblast Hs68 cell lysate
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
_Anonymous_ _15_
WB Human 03/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedCaco-2, T84, HCT116, HT29, DU145, BPH1, Hela
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Eric England
WB Other 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Human 01/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates: CHO, GC-1, Hela, Cos-7, SH-SY5Y
SpeciesHuman
Lot083M4851
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
  5
reviewed by:
Verified Customer
WB Human 12/02/2013
View

Summary

ApplicationWestern Blot
Sample Testedhuman brain cells
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Zi-Jun Lin
WB Human 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line
SpeciesHuman
  5
reviewed by:
Che-Yi Chao
WB Mouse 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse BALB/c macrophage cell line
SpeciesMouse
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Ssu-Ching Wang
WB Mouse 12/14/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Hui-Chen Lin
WB Human 12/08/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
  4
reviewed by:
Chiung-Chi Peng
WB Rat 12/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat
  5
reviewed by:
Shun-Fa Yang
WB Human 12/05/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
  5
reviewed by:
Ping-Sung Chiu
WB Mouse 11/30/2011
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain
SpeciesMouse
  5
reviewed by:
Chien-Hui Yu
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell lines
SpeciesHuman
  5
reviewed by:
Hsien Sheng Tin
WB Other 11/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell lines
SpeciesOther
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 09/02/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse Cells
SpeciesMouse
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Other 08/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedGround squirrel small intestinal lysate, Sample Amount: 50 ug
SpeciesOther
  5
reviewed by:
Verified Customer
WB Mouse 08/30/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate, adult neural stem cell whole cell lysate
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB600-501). (Showing 1 - 10 of 12 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What is the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW for beta Actin antibodies is approximately 42 kDa.
  5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
    • For homogenized tissue, beta-actin and GAPDH are both fine.
  6. We have these Abs: NB120-19481 PPAR gamma Antibody and NB600-501 Beta Actin Antibody (AC-15). The samples that we are analyzing are bovine ones. Please, confirm me if those proteins are valid like positive controls in WB for those antibodies: 1. Beta Actin Recombinant Protein (H00000060-P01). 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). Or perhaps could be better those lysates: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02 3. PPAR gamma Lysate - NBL1-14634 4. PPAR gamma Lysate - NBL1-14633 5. PPAR gamma Lysate - NBL1-14635.
    • I recommend the below controls as we have validated these as controls. NB120-19481: 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). NB600-501: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02
  7. I would like to try the Beta Actin Antibody (AC-15) you have in stock to see if it reacts with fish samples in a Western Blot. Also, I would like to know if you have more information about its reactivity with fish?
    • We have tested that this antibody NB600-501 is reactive with fish. It has been tested with use in black carp. The homology with black carp is 99.0%.
  8. Could anti-beta actin antibody recognize the actin derived from mosquito cells (C6/36)?
    • Unfortunately we have not used our antibody with catalogue number NB600-501 to detect beta actin that is derived from mosquito cells. You may however wish to align the peptide immunogen sequence against the full length mosquito beta actin sequence to check the homology. We usually suggest that a homology of greater than 80% is indicative of likely binding. The immunogen sequence for NB600-501 is Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (DDDIAALVIDNGSGK).
  9. May we ask if NB600-501 has been tested with samples from zebrafish?
    • Beta Actin is a highly conserved protein and yes, our Beta Actin antibody (clone AC-15) NB600-501 is guaranteed for Zebrafish species.
  10. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence (Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys) within the N-terminal region of beta Actin, conjugated to KLH.
  11. Show All 12 FAQs.

Secondary Antibodies

 

Isotype Controls

Additional beta-Actin Products

Bioinformatics Tool for beta-Actin Antibody (NB600-501)

Discover related pathways, diseases and genes to beta-Actin Antibody (NB600-501). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta-Actin Antibody (NB600-501)

Discover more about diseases related to beta-Actin Antibody (NB600-501).
 

Pathways for beta-Actin Antibody (NB600-501)

View related products by pathway.

PTMs for beta-Actin Antibody (NB600-501)

Learn more about PTMs related to beta-Actin Antibody (NB600-501).
 

Research Areas for beta-Actin Antibody (NB600-501)

Find related products by research area.

Blogs on beta-Actin.

Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.8
5
22
4
5
3
0
2
0
1
0

Lindsey Johnson
01/22/2022
Application: ICC
Species: Mouse

Tobias brun
05/16/2021
Application: WB
Species: Human

 
Verified Customer
01/31/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB