beta-Actin Antibody (AC-15)

Images

 
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with doxycycline to control the expression of the gene of interest. Beta actin blocking confirms the albumin assay showing that an equal amount ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - HS-68 cells.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-actin in NIH/3T3 and HEK293T cells using HRP conjugated beta-actin antibody (Cat# NB600-501H). Image from verified customer review.
Immunohistochemistry: beta-Actin Antibody (AC-15) [NB600-501] - Breast, Epithelium 40x.
Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human cell lines (Caco-2, T84, HCT116, HT29, DU145, BPH1, HeLa). Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Immunofluroescence Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta Actin, Clone No. AC-15 (NB600-501).
Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 ...read more
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Simple Western lane view shows a specific band for Beta-Actin in 0.2 mg/ml of Hela, MCF-7, SH-SY5Y, & Jurkat ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, ZeSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
AC-15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

beta-Actin Antibody (AC-15) Summary

Immunogen
slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Epitope
N-terminus of the beta isoform of actin.
Specificity
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:10000
  • Simple Western 1:25
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives. Use in immunoprecipitation reported in scientific literature (PMID 23647384).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size: Wes/Sally Sue/Peggy Sue.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 20 Reviews rated 4.8
using
NB600-501 in the following applications:

Publications
Read Publications using
NB600-501 in the following applications:

  • 1 publication
  • 2 publications
  • 3 publications
  • IHC
    3 publications
  • IP
    2 publications
  • WB
    188 publications

Reactivity Notes

The antibody cross reacts with b-actin expressing cells in carp, leech tissues (Hirudo medicinalis), ground squirrel. Does not cross react with adult cardiac, skeletal muscle, drosophila or amoeba beta actin. Hamster reactivity reported in scientific literature (PMID: 24478435). Mammal reactivity reported in scientific literature (PMID: 25130694). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for beta-Actin Antibody (AC-15)

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • anti-beta actin
  • beta actin (ac-15)
  • Beta Actin
  • beta cytoskeletal actin
  • beta-actin ac 15
  • beta-actin ac15
  • beta-actin ac-15
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
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Publications for beta-Actin Antibody (NB600-501)(283)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Fish, Hamster, Mammal, Porcine, Rabbit, Squirrel, Zebrafish.

We have publications tested in 6 applications: ChIP, ELISA, ICC/IF, IHC, IP, WB.


Filter By Application
ChIP
(1)
ELISA
(2)
ICC/IF
(3)
IHC
(3)
IP
(2)
WB
(188)
All Applications
Filter By Species
Human
(98)
Mouse
(56)
Rat
(19)
Bovine
(1)
Canine
(2)
Chicken
(3)
Fish
(1)
Hamster
(2)
Mammal
(1)
Porcine
(2)
Rabbit
(5)
Squirrel
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 283. Show All 283 Publications.
Publications using NB600-501 Applications Species
El-Hassan AR, Leung V, Kharfallah F et al. Identification and characterization of a novel chemically induced allele at the planar cell polarity gene Vangl2. Mamm. Genome. 2017 Oct 24 [PMID: 29063958] (WB, Mouse) WB Mouse
Zhao W, Pferdehirt L, Segatori L. Quantitatively predictable control of cellular protein levels through proteasomal degradation. ACS Synth Biol. 2017 Oct 23 [PMID: 29061039] (WB) WB
Walters DK, Arendt BK, Tschumper RC et al. Characterization and use of the novel human multiple myeloma cell line MC-b11/14 to study biological consequences of CRISPR-mediated loss of immunoglobulin a heavy chain. Exp. Hematol. 2017 Oct 10 [PMID: 29030084] (Human) Human
Yoon H, Radulovic M, Walters G et al. Protease activated receptor 2 controls myelin development, resiliency and repair Glia 2017 Sep 18 [PMID: 28921694] (Mouse) Mouse
Niu J, Huang D, Zhou R et al. Activation of dorsal horn cannabinoid CB2 receptor suppresses the expression of P2Y12 and P2Y13 receptors in neuropathic pain rats J Neuroinflammation 2017 Sep 12 [PMID: 28899427] (WB, Rat) WB Rat
Nagami M, Ito Y, Nagasawa T. Phenethyl isothiocyanate protects against H2O2-induced insulin resistance in 3T3-L1 adipocytes Biosci. Biotechnol. Biochem. 2017 Sep 13 [PMID: 28899227] (Mouse) Mouse
Chiu HY, Tsay YG, Hung SC. Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture Sci Rep 2017 Aug 31 [PMID: 28860574] (WB, Human) WB Human
Bohnsack JP, Patel VK, Morrow AL. Ethanol exposure regulates Gabra1 expression via histone deacetylation at the promoter in cultured cortical neurons J. Pharmacol. Exp. Ther. 2017 Aug 10 [PMID: 28798030]
Noutsios GT, Willis AL, Ledford JG, Chang EH. Novel role of surfactant protein A in bacterial sinusitis Int Forum Allergy Rhinol 2017 Jul 20 [PMID: 28727907] (Human) Human
Lin NH, Messing A, Perng MD. Characterization of a panel of monoclonal antibodies recognizing specific epitopes on GFAP PLoS ONE 2017 Jul 12 [PMID: 28700643] (WB, Human) WB Human
Show All 283 Publications.

Reviews for beta-Actin Antibody (NB600-501) (20) 4.820

Average Rating: 4.8
(Based on 20 reviews)
We have 20 reviews tested in 4 species: Human, Mouse, Rat, Other.
We have 20 reviews tested in 1 application: WB.

Reviews using NB600-501:
Filter by Applications
WB
(20)
All Applications
Filter by Species
Human
(10)
Mouse
(6)
Rat
(1)
Other
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot beta-Actin NB600-501
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reviewed by:
Liyong Zhang
WB Human 11/25/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 06/05/2015
View

Summary

ApplicationWestern Blot
Sample Testedhuman glioma cells
SpeciesHuman

Blocking

Blocking Details10% milk in TBST

Details

Detection NotesECL western blotting detection reagent
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 05/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedFibroblast Hs68 cell lysate
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 25146190

Details

Detection NotesSee PMID: 25146190

Comments

CommentsEffects of glucose restriction (GR) on the expression of Nampt protein in Hs68 cells. beta-Actin used as a loading control. See PMID: 25146190 for details
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 03/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedCaco-2, T84, HCT116, HT29, DU145, BPH1, Hela
SpeciesHuman

Blocking

Blocking Details1 hour at RT in 5% milk powder solution (in 1xTBST)

Primary Anitbody

Dilution RatioDilution:1/15000, 1 hour at RT

Secondary Antibody

Secondary DescriptionHRP

Details

Detection NotesECL detection
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Other 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/4000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionGoat anti-Mouse 680 RD - Licor Biosciences
Secondary Manufacturer Cat#925-68070
Secondary Concentration1/20,000

Details

Detection NotesLicor Odyssey
Western Blot beta-Actin NB600-501
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reviewed by:
Sandra Vieira
WB Human 01/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates: CHO, GC-1, Hela, Cos-7, SH-SY5Y
SpeciesHuman
Lot083M4851

Blocking

Blocking Detailsblocking solution was added for 1h (5% non-fat dry milk in 1x TBS-T)

Primary Anitbody

Dilution RatioPrimary antibody B-Actin (1:10000), diluted in 3% non-fat dry milk in 1x TBS-T, was added and incubated with membranes 1h

Secondary Antibody

Secondary DescriptionGoat anti-mouse
Secondary Concentration1:5000

Details

Detection Notesthe enhanced chemiluminescence (ECL) detection method; Homemade ECL 1 min; Exposure time: 5 min
Western Blot beta-Actin NB600-501
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reviewed by:
Liyong Zhang
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF
Western Blot beta-Actin NB600-501
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reviewed by:
Beatrice Gini
WB Human 12/02/2013
View

Summary

ApplicationWestern Blot
Sample Testedhuman brain cells
SpeciesHuman
CommentsClin Cancer Res. 2013 Oct 15;19(20):5722-32.

Blocking

Blocking Details5% BSA-TBS 1X-Tween 0.1% in dH2O, 1h, RT

Primary Anitbody

Dilution Ratio1:10000, 1 h, RT, in TBS 1X-Tween 0.1% in dH2O

Secondary Antibody

Secondary Descriptionanti-mouse, 1:5000

Details

Detection NotesThermo detection reagent

Comments

CommentsClin Cancer Res. 2013 Oct 15;19(20):5722-32.
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 12/21/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% FBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Mouse 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse BALB/c macrophage cell line
SpeciesMouse
CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in PBST, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Mouse IgG (HRP), Jackson

Details

Detection NotesDetection Method: GE ImageQuant LAS 4000 mini plus ECL

Comments

CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Mouse 12/14/2011
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Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: HRP System, detect ECL signal by photographic film
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 12/08/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse IgG HRP

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Rat 12/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 1 hour

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Human 12/05/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 3% BSA, Blocking Time: 2 hours

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 16 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse Millipore, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL Detection

Comments

CommentsGood!
 
reviewed by:
Anonymous
WB Mouse 11/30/2011
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat dry milk in PBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Mouse IgG HRP, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell lines
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% TTBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Other 11/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell lines
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-mouse HRP

Details

Detection NotesDetection Method: ECL
Western Blot beta-Actin NB600-501
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reviewed by:
Wei-I Shiao
WB Mouse 09/02/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse Cells
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: HyBlock blocking buffer from HyCell Biotec, Blocking Time: 1 minute

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 10 minutes

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse IgG

Details

Detection NotesDetection Method: Viaglo HRP Chemilum Substrate Kit, Negative Control: Beta Actin
Western Blot beta-Actin NB600-501
Enlarge
reviewed by:
Courtney Kurtz
WB Other 08/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedGround squirrel small intestinal lysate, Sample Amount: 50 ug
SpeciesOther
CommentsAntibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!

Blocking

Blocking DetailsBlocking Buffer: 4% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Incubation Dilution Buffer: 4% milk, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-mouse poly-HRP, Secondary Ab Dilution Ratio: 1:1500

Details

Detection NotesDetection Method: Opti-4CN, Exposure Time: < 10 s, Specific Bands: ~42 kDa

Comments

CommentsAntibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!
 
reviewed by:
WB Mouse 08/30/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate, adult neural stem cell whole cell lysate
SpeciesMouse
CommentsVery strong detection with 1:5000 dilution

Comments

CommentsVery strong detection with 1:5000 dilution

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB600-501). (Showing 1 - 9 of 9 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. We have these Abs: NB120-19481 PPAR gamma Antibody and NB600-501 Beta Actin Antibody (AC-15). The samples that we are analyzing are bovine ones. Please, confirm me if those proteins are valid like positive controls in WB for those antibodies: 1. Beta Actin Recombinant Protein (H00000060-P01). 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). Or perhaps could be better those lysates: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02 3. PPAR gamma Lysate - NBL1-14634 4. PPAR gamma Lysate - NBL1-14633 5. PPAR gamma Lysate - NBL1-14635.
    • I recommend the below controls as we have validated these as controls. NB120-19481: 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). NB600-501: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02
  6. I would like to try the Beta Actin Antibody (AC-15) you have in stock to see if it reacts with fish samples in a Western Blot. Also, I would like to know if you have more information about its reactivity with fish?
    • We have tested that this antibody NB600-501 is reactive with fish. It has been tested with use in black carp. The homology with black carp is 99.0%.
  7. Could anti-beta actin antibody recognize the actin derived from mosquito cells (C6/36)?
    • Unfortunately we have not used our antibody with catalogue number NB600-501 to detect beta actin that is derived from mosquito cells. You may however wish to align the peptide immunogen sequence against the full length mosquito beta actin sequence to check the homology. We usually suggest that a homology of greater than 80% is indicative of likely binding. The immunogen sequence for NB600-501 is Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (DDDIAALVIDNGSGK).
  8. May we ask if NB600-501 has been tested with samples from zebrafish?
    • Beta Actin is a highly conserved protein and yes, our Beta Actin antibody (clone AC-15) NB600-501 is guaranteed for Zebrafish species.
  9. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence (Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys) within the N-terminal region of beta Actin, conjugated to KLH.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 550 Labeled NB600-501R
DyLight 650 Labeled NB600-501C
HRP Labeled NB600-501H

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Blogs on beta-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Liyong Zhang
11/25/2017
Application: WB
Species: Human

Anonymous
06/05/2015
Application: WB
Species: Human

Anonymous
05/29/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB
Entrez