beta-Actin Antibody (AC-15)

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.

Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e, red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-Beta Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Beta-actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.

Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (orange) and Isotype control antibody (blue).

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - GFAP and IL-6 are elevated in cultures of spinal cord compared to cortical astrocytes. ((G, H) Analysis of protein homogenates from brain or spinal cord astrocyte cultures also demonstrated higher levels of GFAP expression, but no differences in ALDH1L1. Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0180697), licensed under a CC-BY licence.

Immunohistochemistry: beta-Actin Antibody (AC-15) [NB600-501] - Breast, Epithelium 40X.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 for 1 hour. Image from verified customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MMP-11 expression in oral cancer cell lines and the MMP-11 knockdown in SCC9 cells reduce cell migration. Western blot analysis showing the expression of MMP-11 protein in five oral cancer cell lines. Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113129), licensed under a CC-BY licence.

Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HSP90 and HSP70 interact with PABPN1. Interaction of PABPN1 with HSP90. Lysates from HEK293 cells transfected with Flag-HSP90 and GFP-tagged A17-PABPN1 were immunoprecipitated with an anti-Flag antibody. Immunoprecipitates (IP) were analyzed by Western blot (IB).Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0138936), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - HS-68 cells.

Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-)
• Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO
• Clone: AC-15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

beta-Actin Antibody (AC-15) Summary

• Immunogen: This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Epitope: N-terminus of the beta isoform of actin.
• Specificity: In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixing and paraffin-embedding.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
  • Simple Western 1:25
  • Western Blot 1:5000 - 1:10000
• Application Notes: Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:25-1:100; matrix was 12-230 kDa.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antibody cross reacts with beta-actin expressing cells in carp, leech tissues (Hirudo medicinalis), ground squirrel. Does not cross react with adult cardiac, skeletal muscle, drosophila or amoeba beta actin. Mammal reactivity and Hamster reactivity reported in scientific literature (PMID: 25130694 and 24478435 respectively). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Human reactivity reported in scientific literature (PMID:32938681) Use in Turkey reported in scientific literature (PMID:27816932).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 15mM Sodium Azide
• Purity: Unpurified

Alternate Names for beta-Actin Antibody (AC-15)

• ACTB
• actin, beta
• actin, cytoplasmic 1
• anti-beta actin
• B-Actin
• beta actin (ac-15)
• Beta Actin
• beta cytoskeletal actin
• beta-actin ac 15
• beta-actin ac15
• beta-actin ac-15
• betaActin
• beta-Actin
• BRWS1
• I(2)-Actin
• PS1TP5-binding protein 1
• PS1TP5BP1

Background

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Actin Antibody (NB600-501)(480)

Publications using NB600-501

• Chen Y, Le P, Tzeng T Et al. Graptopetalum paraguayense Extract Ameliorates Proteotoxicity in Aging and Age-Related Diseases in Model Systems Nutrients Nov 29 2021 [PMID: 34959868] (WB, Human)
• Pagano A, Breuzard G, Parat F Et al. Tau Regulates Glioblastoma Progression, 3D Cell Organization, Growth and Migration via the PI3K-AKT Axis Cancers Nov 19 2021 [PMID: 34830972] (WB, IHC-Fr, Human)
• McCorkell KA, Jayachandran N, Cully MD Et al. Lymph node formation and B cell homeostasis require IKK-alpha in distinct endothelial cell-derived compartments Proceedings of the National Academy of Sciences of the United States of America Nov 30 2021 [PMID: 34810256]
• Wang K, Chen PN, Chien HW Et al. Demethoxycurcumin inhibits the cell migration and MMP-2 expression in human retinal pigment epithelial cells by targeting the STAT-3 pathway Experimental eye research Nov 15 2021 [PMID: 34793827] (WB, Human)
• Tornabene P, Trapani I, Centrulo M Et al. Inclusion of a degron reduces levelsof undesired inteins after AAV-mediated proteintrans-splicing in the retina Molecular Therapy - Methods & Clinical Development Dec 1 2021 [PMID: 34786437] (WB, Mouse, Porcine)
• Liao GR, Tseng YY, Tseng CY Et al. The orf virus (ORFV) protein OV20.0 interacts with the microprocessor complex subunit DGCR8 to regulate miRNA biogenesis and ORFV infection FEBS letters Nov 14 2021 [PMID: 34778960]
• Wang Y, Sun L, Wang L Et al. PUM1 modulates trophoblast cell proliferation and migration through LRP6 Biochemistry and cell biology = Biochimie et biologie cellulaire Dec 1 2021 [PMID: 34734756]
• Mehanna ET, Khalaf SS, Mesbah NM Et al. Anti-oxidant, anti-apoptotic, and mitochondrial regulatory effects of selenium nanoparticles against vancomycin induced nephrotoxicity in experimental rats Life sciences Oct 26 2021 [PMID: 34715137] (WB, Rat)
• Sevilleja-Ortiz A, El Assar M, GarcIa-Rojo E Et al. Ageing-induced hypercontractility is related to functional enhancement of STIM/Orai and upregulation of Orai 3 in rat and human penile tissue Mechanisms of ageing and development Oct 23 2021 [PMID: 34699858] (WB, Human, Rat)
• Chiu H, Chiu HP, Yu HP Et al. Zinc finger protein ZFP36L1 inhibits flavivirus infection by both 5'-3' XRN1 and 3'-5' RNA-exosome RNA decay pathways Journal of virology Oct 13 2021 [PMID: 34643435]
• Alam S, Mohammad T, Padder RA Et al. Thymoquinone and quercetin induce enhanced apoptosis in non-small cell lung cancer in combination through the Bax/Bcl2 cascade Journal of cellular biochemistry Oct 12 2021 [PMID: 34636440]
• Yoon H, Triplet EM, Simon WL Et al. Blocking Kallikrein 6 promotes developmental myelination Glia Oct 9 2021 [PMID: 34626143]
• Chiang MK, Hsiao PY, Liu YY Et al. Two ST11 Klebsiella pneumoniae strains exacerbate colorectal tumorigenesis in a colitis-associated mouse model Gut microbes Oct 4 2021 [PMID: 34606408] (WB, Mouse)
• Kuraoka S, Higashi H, Yanagihara Y et al. A Novel Spectral Annotation Strategy Streamlines Reporting of mono-ADP-ribosylated Peptides Derived from Mouse Liver and Spleen in Response to IFN- gamma Molecular & cellular proteomics : MCP Sep 27 2021 [PMID: 34592425] (WB)
• Martin CE, Murray AS, Sala-Hamrick KE et al. Post-translational modifications of serine protease TMPRSS13 regulate zymogen activation, proteolytic activity, and cell surface localization The Journal of biological chemistry Sep 22 2021 [PMID: 34562451] (WB, Human)
• Wang HI, Chuang ZS, Kao YT Et al. Small Structural Proteins E and M Render the SARS-CoV-2 Pseudovirus More Infectious and Reveal the Phenotype of Natural Viral Variants International journal of molecular sciences Aug 23 2021 [PMID: 34445789]
• Cheng CY, Chiang SY, Kao ST, Huang SC Alpinia oxyphylla Miq extract reduces cerebral infarction by downregulating JNK-mediated TLR4/T3JAM- and ASK1-related inflammatory signaling in the acute phase of transient focal cerebral ischemia in rats Chinese medicine Aug 21 2021 [PMID: 34419138] (WB, Rat)
• Lamprou I, Tsolou A, Kakouratos C Et al. Suppressed PLIN3 frequently occurs in prostate cancer, promoting docetaxel resistance via intensified autophagy, an event reversed by chloroquine Medical oncology (Northwood, London, England) Aug 19 2021 [PMID: 34410522] (WB, Human)
• Gagnon J, Caron V, Gyenizse L, Tremblay A Atypic SUMOylation of Nor1/NR4A3 regulates neural cell viability and redox sensitivity FASEB journal : official publication of the Federation of American Societies for Experimental Biology Sep 1 2021 [PMID: 34383980]
• Beckermann Tm, Luo W, Wilson Cm Et Al. Cognate restriction of transposition by piggyBac-like proteins Nucleic acids research Jul 7 2021 [PMID: 34232995] (WB)
• Hsieh MC, Lo YS, Chuang YC et al. Dehydrocrenatidine extracted from Picrasma quassioides induces the apoptosis of nasopharyngeal carcinoma cells through the JNK and ERK signaling pathways Oncology reports Aug 1 2021 [PMID: 34165177] (WB, Human)
• Oyama Y, Shigeta S, Tokunaga H et al. CHD4 regulates platinum sensitivity through MDR1 expression in ovarian cancer: A potential role of CHD4 inhibition as a combination therapy with platinum agents PloS one Jun 23 2021 [PMID: 34161330] (WB, Human)
• Liu WH, VOlse K, Senft D, Jeremias I A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models Scientific reports Jun 16 2021 [PMID: 34135367] (Flow, Human)
• Cheng CY, Huang HC, Kao ST, Lee YC Angelica sinensis extract promotes neuronal survival by enhancing p38 MAPK-mediated hippocampal neurogenesis and dendritic growth in the chronic phase of transient global cerebral ischemia in rats Journal of ethnopharmacology Jun 4 2021 [PMID: 34090910]
• Chuang Y, Hsieh M, Lin C et al. Pinosylvin inhibits migration and invasion of nasopharyngeal carcinoma cancer cells via regulation of epithelial mesenchymal transition and inhibition of MMP 2 Oncology Reports May 27 2021 [PMID: 34080661] (WB, Human)
• Hosseini-Farahabadi S, Baradaran-Heravi A, Zimmerman C et al. Small molecule Y-320 stimulates ribosome biogenesis, protein synthesis, and aminoglycoside-induced premature termination codon readthrough PLoS biology May 1 2021 [PMID: 33939688] (WB, Human)
• Liao GR, Tseng YY, Tseng CY et al. K160 in the RNA-binding domain of the orf virus virulence factor OV20.0 is critical for its functions in counteracting host antiviral defense FEBS letters Apr 28 2021 [PMID: 33909294]
• Chang CW, Chiu CH, Lin MH et al. GMP-compliant fully automated radiosynthesis of [18F]FEPPA for PET/MRI imaging of regional brain TSPO expression EJNMMI research Mar 16 2021 [PMID: 33725191] (WB, Mouse)
• Wu YH, Yang Y, Chen CH et al. Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2 PLoS pathogens Mar 1 2021 [PMID: 33720996] (WB, Human)
• Triplet EM, Kim HN, Yoon H et al. The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury Neurobiology of disease Feb 5 2021 [PMID: 33549720] (WB, Mouse)
• Morton RE, Mihna D, Liu Y The lipid substrate preference of CETP controls the biochemical properties of HDL in fat/cholesterol-fed hamsters Journal of lipid research Jan 27 2021 [PMID: 33515552] (WB)
• Cai C, Tahiri H, Fortin C, et al. Lymphocytic microparticles suppress retinal angiogenesis via targeting Müller cells in the ischemic retinopathy mouse model Experimental cell research Feb 15 2021 [PMID: 33434529] (WB, Rat)
• Tsochantaridis I, Roupas A, Voulgaridou GP, et al. Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells Biomedicines Jan 6 2021 [PMID: 33419031] (WB, Human)
• Gul HF, Ilhan N, Ilhan N et al. The Combined Effect of Pomegranate Extract and Tangeretin on the DMBA-induced Breast Cancer Model The Journal of nutritional biochemistry Dec 13 2020 [PMID: 33326843] (WB, Rat)
• Galli A, Marciani P, Marku A et al. Verbascoside Protects Pancreatic beta -Cells against ER-Stress Biomedicines Dec 8 2020 [PMID: 33302345] (WB, Mouse)
• Byts N, Sharma S, Laurila J et al. Transmembrane Prolyl 4-Hydroxylase Is a Novel Regulator of Calcium Signaling in Astrocytes eNeuro Dec 8 2020 [PMID: 33298456]
• Lee YC, Kao ST, Cheng CY Acorus tatarinowii Schott extract reduces cerebral edema caused by ischemia-reperfusion injury in rats: involvement in regulation of astrocytic NKCC1/AQP4 and JNK/iNOS-mediated signaling BMC complementary medicine and therapies Dec 9 2020 [PMID: 33298024] (Rat)
• Chao CT, Yeh HY, Tsai YT et al. Astaxanthin Counteracts Vascular Calcification In Vitro Through an Early Up-Regulation of SOD2 Based on a Transcriptomic Approach Int J Mol Sci Nov 12 2020 [PMID: 33198315] (WB, Rat)
• Fenzl A, Kulterer OC, Spirk K et al. Intact vitamin A transport is critical for cold-mediated adipose tissue browning and thermogenesis Mol Metab Sep 26 2020 [PMID: 32992038] (WB, Mouse)
• Liu WH, Mrozek-Gorska P, Wirth AK et al. Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL Biomarker research Sep 16 2020 [PMID: 32944247] (WB)
• Zou Y, Henry WS, Ricq EL et al. Plasticity of ether lipids promotes ferroptosis susceptibility and evasion Nature Sep 1 2020 [PMID: 32939090]
• Rogers MA, Buffolo F, Schlotter F et al. Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Science advances Sep 1 2020 [PMID: 32938681] (WB, Human)
• Giatromanolaki A, Kouroupi M, Pouliliou S et al. Ectonucleotidase CD73 and CD39 expression in non-small cell lung cancer relates to hypoxia and immunosuppressive pathways Life Sci. Sep 6 2020 [PMID: 32898522] (WB, Human)
• Gao N, Zhao K, Cao Y et al. A role of lamin A/C in preventing neuromuscular junction decline in mice J. Neurosci. Aug 17 2020 [PMID: 32817327] (WB)
• Varela FA, Foust VL, Hyland TE et al. TMPRSS13 promotes cell survival, invasion, and resistance to drug-induced apoptosis in colorectal cancer Sci Rep Aug 17 2020 [PMID: 32807808] (WB, Mouse)
• Park HJ, Jo DS, Choi DS et al. Ursolic acid inhibits pigmentation by increasing melanosomal autophagy in B16F1 cells Biochem. Biophys. Res. Commun. Aug 10 2020 [PMID: 32792197] (WB, Mouse)
• Liu J, Zhu C, Zhang L et al. MicroRNA-1469-5p promotes the invasion and proliferation of pancreatic cancer cells via direct regulating the NDRG1/NF-kappa B/E-cadherin axis Hum. Cell Aug 5 2020 [PMID: 32757096]
• Lee JY, Fan CC, Chou NL et al. PHRF1 promotes migration and invasion by modulating ZEB1 expression PLoS ONE Jul 30 2020 [PMID: 32730336] (IHC-P, Human)
• Cinque L, De Leonibus C, Iavazzo M et al. MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B EMBO J. Sep 1 2020 [PMID: 32716134] (WB, Mouse)
• La Fuente JM, Sevilleja-Ortiz A, Garcia-Rojo E et al. Erectile dysfunction is associated with defective L-cysteine/hydrogen sulfide pathway in human corpus cavernosum and penile arteries Eur. J. Pharmacol. Jul 23 2020 [PMID: 32712093] (WB, Human)
• Chi TH, Hsieh BY, Liang PS et al. Expression and Functional Study of Single Mutations of Carbonic Anhydrase 8 in Neuronal Cells Cell. Mol. Neurobiol. Jun 24 2020 [PMID: 32583043]
• Beauvarlet J, Nath Das R, Alvarez-Valadez K et al. Triarylpyridine Compounds and Chloroquine Act in Concert to Trigger Lysosomal Membrane Permeabilization and Cell Death in Cancer Cells Cancers (Basel) Jun 18 2020 [PMID: 32570977]
• Khodja Y, Samuels ME Ethanol-mediated upregulation of APOA1 gene expression in HepG2 cells is independent of de novo lipid biosynthesis Lipids Health Dis Jun 20 2020 [PMID: 32563265] (WB, Human)
• Wu CL, Chang CC, Yang TH et al. Tubular transcriptional co-activator with PDZ-binding motif protects against ischemic acute kidney injury Clin. Sci. Jul 17 2020 [PMID: 32558891] (Mouse, Human)
• Hsieh M, Hsieh M, Lo Y et al. Modulating effect of Coronarin D in 5-fluorouracil resistance human oral cancer cell lines induced apoptosis and cell cycle arrest through JNK1/2 signaling pathway Biomedicine & Pharmacotherapy Aug 1 2020 [PMID: 32502840] (WB, Human)
• Alicea GM, Rebecca VW, Goldman AR et al. Changes in Aged Fibroblast Lipid Metabolism Induce Age-dependent Melanoma Cell Resistance to Targeted Therapy Via the Fatty Acid Transporter FATP2 Cancer Discov Jun 4 2020 [PMID: 32499221]
• Marshall J, Courage E, Elliott R et al. THP-1 Macrophage Cholesterol Efflux Is Impaired by Palmitoleate Through Akt Activation PLoS One. 2020 May 21 [PMID: 32437392] (WB, Human)
• Oishi N, Hundal T, Phillips JL et al. Molecular profiling reveals a hypoxia signature in breast implant-associated anaplastic large cell lymphoma Haematologica May 15 2020 [PMID: 32414854] (WB, Human)
• Chang Y, Ko B, Ju J et al SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation. 2020 Apr 30 [PMID: 32365944] (WB, DB, Human)
• Jin Y, Shen Y, Su X et al. The Small GTPases Rab27b Regulates Mitochondrial Fatty Acid Oxidative Metabolism of Cardiac Mesenchymal Stem Cells Front Cell Dev Biol Apr 15 2020 [PMID: 32351955] (WB, Mouse)
• Barbier V, Erbani J, Fiveash C et al. Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance Nat Commun Apr 27 2020 [PMID: 32341362] (WB, Mouse)
• Li R, Li X, Hagood J et al. Myofibroblast contraction is essential for generating and regenerating the gas-exchange surface J. Clin. Invest. Apr 27 2020 [PMID: 32338642] (WB, Mouse)
• Lieberman O J, Cartocci V et al. mTOR Suppresses Macroautophagy During Striatal Postnatal Development and Is Hyperactive in Mouse Models of Autism Spectrum Disorders. Front Cell Neurosci Apr 17 2020 [PMID: 32296308] (WB, Mouse)
• Romeo MA, Gilardini Montani MS, Benedetti R et al. PBA Preferentially Impairs Cell Survival of Glioblastomas Carrying mutp53 by Reducing Its Expression Level, Stabilizing wtp53, Downregulating the Mevalonate Kinase and Dysregulating UPR Biomolecules Apr 10 2020 [PMID: 32290231]
• Murthi P, Rajaraman G et al. Decreased Placental FPR2 in Early Pregnancies That Later Developed Small-For-Gestation Age: A Potential Role of FPR2 in the Regulation of Epithelial-Mesenchymal Transition. Cells Oct 4 2020 [PMID: 32290034] (WB, Human)
• Pei X, Kim H, Lee M et al. Local delivery of cardiac stem cells overexpressing HIF-1 alpha promotes angiogenesis and muscular tissue repair in a hind limb ischemia model J Control Release Mar 16 2020 [PMID: 32194175] (WB, Rat)
• Lin Jh, Chen Sy, Lu Cc et Al. Ursolic acid promotes apoptosis, autophagy, and chemosensitivity in gemcitabine-resistant human pancreatic cancer cells Phytother Res Mar 17 2020 [PMID: 32185829] (WB)
• Laitakari A, Huttunen R, Kuvaja P et al. Systemic long-term inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 ameliorates aging-induced changes in mice without affecting their life span FASEB J. Feb 25 2020 [PMID: 32100354] (WB, Mouse)
• Chen YJ, Tsai CY, Cheng YM et al. Impacts of Intralipid on Nanodrug Abraxane Therapy and on the Innate Immune System Sci Rep Feb 18 2020 [PMID: 32071352] (WB, Mouse)
• Cheng CY, Kao ST, Lee YC Angelica sinensis extract protects against ischemia-reperfusion injury in the hippocampus by activating p38 MAPK-mediated p90RSK/p-Bad and p90RSK/CREB/BDNF signaling after transient global cerebral ischemia in rats J Ethnopharmacol Jan 24 2020 [PMID: 31988015] (WB, Rat)
• Giatromanolaki A, Fasoulaki V et al. CYP17A1 and Androgen-Receptor Expression in Prostate Carcinoma Tissues and Cancer Cell Lines. Curr Urol Jan 11 2019 [PMID: 31933595] (WB, Human)
• Biswas L, Zeng Z, Graham A, Shu X Gypenosides mediate cholesterol efflux and suppress oxidized LDL induced inflammation in retinal pigment epithelium cells Exp. Eye Res. Jan 10 2020 [PMID: 31931003] (WB, Human)
• Lieberman OJ, Frier MD, McGuirt AF et al. Cell-type-specific regulation of neuronal intrinsic excitability by macroautophagy Elife Jan 8 2020 [PMID: 31913125]
• Cho S, Chae J, Shin H, et al. Enhanced Anticancer Effect of Adding Magnesium to Vitamin C Therapy: Inhibition of Hormetic Response by SVCT-2 Activation Translational Oncology Dec 31 2019 [PMID: 31901552] (WB, Mouse)
• Alhakamy NA, Md S Repurposing Itraconazole Loaded PLGA Nanoparticles for Improved Antitumor Efficacy in Non-Small Cell Lung Cancers Pharmaceutics Dec 16 2019 [PMID: 31888155] (WB, Human)
• Dos Santos Ortolan L, Sercundes MK, Moura GC et al. Endothelial Protein C Receptor Could Contribute to Experimental Malaria-Associated Acute Respiratory Distress Syndrome J Immunol Res Dec 4 2019 [PMID: 31871954] (WB, Mouse)
• Rogers MA, Chen J, Nallamshetty S et al. Retinoids Repress Human Cardiovascular Cell Calcification With Evidence for Distinct Selective Retinoid Modulator Effects Arterioscler. Thromb. Vasc. Biol. Dec 19 2019 [PMID: 31852220] (WB, Human)
• Langley MR, Yoon H, Kim HN, et al. High fat diet consumption results in mitochondrial dysfunction, oxidative stress, and oligodendrocyte loss in the central nervous system Biochim Biophys Acta Mol Basis Dis Dec 6 2019 [PMID: 31816440] (WB, Mouse)
• Lima FA, Barateiro A, Dombrowski JG Plasmodium falciparum infection dysregulates placental autophagy PLoS ONE Dec 5 2019 [PMID: 31805150] (WB, Human)
• Su X, Jin Y, Shen Y et al. RNAase III-Type Enzyme Dicer Regulates Mitochondrial Fatty Acid Oxidative Metabolism in Cardiac Mesenchymal Stem Cells Int J Mol Sci Nov 7 2019 [PMID: 31703292] (WB, KO, Human, Mouse)
• Lin M C, Shen C H et al. Inhibition of human lung adenocarcinoma growth and metastasis by JC polyomavirus-like particles packaged with an SP-B promoter-driven CD59-specific shRNA. Clin Sci (Lond) Nov 15 2019 [PMID: 31693732] (WB, Human)
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  Details:
  Citation using the HRP form of this antibody.
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  Details:
  Meleagris gallopavo (Turkey)
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• Leinonen H, Rossi M, Salo AM et al. Lack of P4H-TM in mice results in age-related retinal and renal alterations. Hum. Mol. Genet. Jul 27 2016 [PMID: 27466183] (WB, Mouse)
• Sato T, Ito Y, Nagasawa T. Regulatory effects of the L-lysine metabolites, L-2-aminoadipic acid and L-pipecolic acid, on protein turnover in C2C12 myotubes Biosci. Biotechnol. Biochem. 2016 Jul 18 [PMID: 27427787]
• Wong AY, Hristova E, Ahlskog N et al. ABERRANT SUBCELLULAR DYNAMICS OF SIGMA-1 RECEPTOR MUTANTS UNDERLYING NEUROMUSCULAR DISEASES Mol. Pharmacol. 2016 Jul 14 [PMID: 27418673] (WB, Human)
• Ekser B, Mangus RS, Kubal CA et al. Recurrence of Hyperoxaluria and Kidney Disease after Combined Intestine-Kidney Transplantation for Enteric Hyperoxaluria Am. J. Nephrol. 2016 Jul 14 [PMID: 27410399] (WB, Mouse)
• Baradaran-Heravi Alireza, Balgi Aruna D, Zimmerman Carla et al. Novel small molecules potentiate premature termination codon readthrough by aminoglycosides. Nucleic Acids Research 2016 [PMID: 27407112] (WB, Human)
• Lee MC, Huang HJ, Chang TH et al. Genome-wide analysis of HIF-2alpha chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions. Sci Rep 2016 Jul 04 [PMID: 27373565] (WB)
• Xu Y, Li F, Zalzala M et al. Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice Hepatology 2016 Jun 30 [PMID: 27359351] (WB)
• Goettsch C, Hutcheson JD, Hagita S et al. A single injection of gain-of-function mutant PCSK9 adeno-associated virus vector induces cardiovascular calcification in mice with no genetic modification. Atherosclerosis Jun 9 2016 [PMID: 27318830] (WB, Mouse)
• Boddicker R L, Razidlo G L et al. Integrated mate-pair and RNA sequencing identifies novel, targetable gene fusions in peripheral T-cell lymphoma. Blood Jan 9 2016 [PMID: 27297792] (WB, Human)
• Tsai WL, Yeh PH, Tsai CY et al. Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. May 24 2016 [PMID: 27218433] (WB, Human)
  
  Details:
  beta-Actin antibody used as a loading control for WB on human samples (protein lysates from differentiating cells – hLiver, Huh7.5.1 and HepG2 cells) (Fig. 2C).
• Bhoopathi P, Lee N, Pradhan AK et al. mda-7/IL-24 induces cell death in neuroblastoma through a novel mechanism involving AIF and ATM. Cancer Research. 2016 Apr 13 [PMID: 27197168] (WB, Human)
  
  Details:
  Beta actin antibody was used for Western blot application in experiments involving human neuroblastoma cancer cell lines namely SK-N-AS, SK-NSH and NB1691.
• Pastore N, Brady OA, Diab HI et al. TFEB and TFE3 Cooperate in the Regulation of the Innate Immune Response in Activated Macrophages. Autophagy. May 12 2016 [PMID: 27171064] (WB, Mouse)
• Radulovic M, Yoon H, Wu J et al. Targeting the thrombin receptor modulates inflammation and astrogliosis to improve recovery after spinal cord injury. Neurobiol. Dis. May 1 2016 [PMID: 27145117] (WB, Mouse)
• Boothe T, Lim GE, Cen H et al. Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells. Mol Metab 2016 May [PMID: 27110488] (WB)
• Lai CW, Chen HL, Tsai TC et al. Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling. Sci Rep. 2016 Apr 18 [PMID: 27087367] (WB, Mouse)
• Xu J, Xu Y, Li Y et al. Carboxylesterase 1 Is Regulated by Hepatocyte Nuclear Factor 4alpha and Protects Against Alcohol- and MCD diet-induced Liver Injury. Sci Rep. 2016 Apr 14 [PMID: 27075303] (WB, Mouse)
• Acharya M, Sokolovska A, Tam JM et al. alphav Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells. Nat Commun. 2016 Mar 11 [PMID: 26965188] (WB, Mouse)
• Naik E, Dixit VM. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCbeta Kinase Activity. J. Immunol. 2016 Mar 02 [PMID: 26936881] (WB, Mouse)
  
  Details:
  LDLR antibody was used for WB detection of Low-density lipoprotein receptorin lysates from liver tissues of Seipin?/? and wild type mice (Fig. 1E). LDLR was found to be highly unregulated in Seipin?/? mice compated to WT mice.
• Hong MN, Nam KY, Kim KK et al. The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha. Cell Stress Chaperones 2016 May [PMID: 26873011]
• Andreev E, Brosseau N, Carmona E et al. The human organic cation transporter OCT1 mediates high affinity uptake of the anticancer drug daunorubicin Sci Rep Feb 10 2016 [PMID: 26861753] (WB, Human)
• Gosejacob D, Jager PS, Vom Dorp K et al. Ceramide Synthase 5 Is Essential to Maintain C16:0-Ceramide Pools and Contributes to the Development of Diet-induced Obesity. J. Biol. Chem. Mar 25 2016 [PMID: 26853464] (WB, ELISA, Mouse)
• Rahtu-Korpela L, Maatta J et al. Hypoxia-Inducible Factor Prolyl 4-Hydroxylase-2 Inhibition Protects Against Development of Atherosclerosis. Arterioscler Thromb Vasc Biol Jan 4 2016 [PMID: 26848160] (WB, Mouse)
• Yoon H, Kleven A, Paulsen A et al. Interplay between exercise and dietary fat modulates myelinogenesis in the central nervous system Biochim. Biophys. Acta Jan 26 2016 [PMID: 26826016] (WB, Mouse)
• Maj M, Hoermann G, Rasul S et al. The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells. J Diabetes Res 2016 [PMID: 26824039] (WB)
• Westwell-Roper C, Denroche HC, Ehses JA, Verchere CB et al. Differential activation of innate immune pathways by distinct islet amyloid polypeptide (IAPP) aggregates J. Biol. Chem. Jan 19 2016 [PMID: 26786104] (WB, Mouse)
• Bohnsack JP, Carlson SL, Morrow AL. Differential regulation of synaptic and extrasynaptic alpha4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology 2016 Jun [PMID: 26767953]
• Davis SM, Collier LA, Leonardo CC et al. Leukemia Inhibitory Factor Protects Neurons from Ischemic Damage via Upregulation of Superoxide Dismutase 3. Mol. Neurobiol. 2016 Jan 09 [PMID: 26746670] (WB, Rat)
• Lee SB, Frattini V, Bansal M et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016 Jan 14 [PMID: 26735018] (WB, Human)
• Cerqueira FM, Chausse B, Baranovski BM et al. Diluted Sera From Calorie Restricted Animals Promote Mitochondrial Beta-Cell Adaptations and Protect Against Glucolipotoxicity. FEBS J. 2016 Jan 06 [PMID: 26732506] (WB, Rat)
  
  Details:
  Beta-Actin antibody used for WB as a loading control on serum from AL and CR rats at a dilution of 1:2000 (Figure 3A).
• Lee KC, Hsieh YC, Yang YY et al. Aliskiren Reduces Hepatic steatosis and Epididymal Fat Mass and Increases Skeletal Muscle Insulin Sensitivity in High-Fat Diet-Fed Mice. Sci Rep 2016 Jan 06 [PMID: 26732252] (WB)
• Kral R, Doriguzzi A, Mayer CE et al. Differential Effects of Variations at Codon 106 on Sprouty2 Functions in Lung Cancer-Derived Cells. J. Cell. Biochem. 2016 Jan 05 [PMID: 26727965] (WB, Human)
  
  Details:
  Beta-Actin antibody used as a loading control for WB on WI-38, A549 and VL-8 cells that were infected with adenoviruses (Figure 1A).
• Yu CY, Liang JJ, Li JK et al. Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins. PLoS Pathog. 2015 Dec 01 [PMID: 26717518] (WB, Human)
• Wang TK, Lin YM, Lo CM et al. Oncogenic roles of carbonic anhydrase 8 in human osteosarcoma cells. Tumour Biol. 2015 Dec 28 [PMID: 26711783] (WB, Human)
  
  Details:
  beta-Actin antibody (clone AC-15) was used for WB assays in experiments involving human osteosarcoma cell lines MG-63, U-2 OS, HOS and 143B and human embryonic kidney cell line HEK293.
• Suriben R, Kaihara KA, Paolino M et al. beta-Cell Insulin Secretion Requires the Ubiquitin Ligase COP1. Cell. 2015 Dec 03 [PMID: 26627735] (WB, Mouse)
• Lin CL, Cheng YS, Li HH et al. Amyloid-beta suppresses AMP-activated protein kinase (AMPK) signaling and contributes to alpha-synuclein-induced cytotoxicity. Exp Neurol 2016 Jan [PMID: 26515689]
• Lin CW, Chen YS, Lin CC et al. Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy. Sci Rep. 2015 Oct 30 [PMID: 26515640] (WB, Mouse)
• Weng WH, Leung WH, Pang YJ, Hsu HH Lauric acid can improve the sensitization of Cetuximab in KRAS/BRAF mutated colorectal cancer cells by retrievable microRNA-378 expression. Oncol Rep 2016 Jan [PMID: 26496897]
• Lee Y, Huang H, Kao S, Cheng C Angelica Sinensis Extract Protects Against Memory Deficits by Promoting P38 MAPK mediated Hippocampal Neurogenesis and Neuronal Survival in the Chronic Phase of Transient Global Cerebral Ischemia in Rats Research Square Sep 17 2020 (Rat)
• Laukka T, Mariani CJ, Ihantola T et al. Fumarate and Succinate Regulate Expression of Hypoxia-inducible Genes via TET Enzymes. J Biol Chem 2016 Feb 19 [PMID: 26703470]
• Vannuvel K, Van Steenbrugge M, Demazy C et al. Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population. J. Cell. Physiol. 2015 Dec 17 [PMID: 26680008] (WB, Human)
• Sorrentino NC, Maffia V, Strollo S et al. A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs. Mol. Ther. 2015 Dec 07 [PMID: 26639405] (WB, Porcine)
• Yang Y, Jin Y, Du W. Programmed cell death 2 functions as a tumor suppressor in osteosarcoma. Int J Clin Exp Pathol. 2015 Nov 30 [PMID: 26617804] (WB, Rat)
• Fossati Silvia, Giannoni Patrizia, Solesio Maria E et al. The carbonic anhydrase inhibitor methazolamide prevents amyloid beta-induced mitochondrial dysfunction and caspase activation protecting neuronal and glial cells in vitro and in the mouse brain Neurobiol Dis 2016 Feb 1 [PMID: 26581638] (WB, Mouse)
  
  Details:
  Citation using the Purified form of this antibody.
• Cheng YF, Young GH, Lin JT et al. Activation of AMP-Activated Protein Kinase by Adenine Alleviates TNF-Alpha-Induced Inflammation in Human Umbilical Vein Endothelial Cells. PLoS ONE. 2015 Nov 07 [PMID: 26544976] (WB, Human)
• Karsikas S, Myllymaki M, Heikkila M et al. HIF-P4H-2 deficiency protects against skeletal muscle ischemia-reperfusion injury. J Mol Med (Berl) 2016 Mar [PMID: 26452676]
• Shi C, Huang X, Zhang B et al. The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein. PLoS One 2015 [PMID: 26414348] (ChIP)
• Qiu Q, Yang C, Xiong W et al. SYK is a target of lymphocyte-derived microparticles in the induction of apoptosis of human retinoblastoma cells. Apoptosis 2015 Dec [PMID: 26404525] (Human)
• Cox JV, Abdelrahman YM, Peters J et al. Chlamydia trachomatis utilizes the mammalian CLA1 lipid transporter to acquire host phosphatidylcholine essential for growth Cell Microbiol. 2016 Mar18 [PMID: 26381674] (WB, Human)
  
  Details:
  Citation using the Purified form of this antibody.
• Koukourakis MI, Kalamida D, Giatromanolaki A et al. Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. PLoS ONE 2015 Sep 18 [PMID: 26378792] (WB, Human)
• Sato T, Ito Y, Nagasawa T. Dietary l-Lysine Suppresses Autophagic Proteolysis and Stimulates Akt/mTOR Signaling in the Skeletal Muscle of Rats Fed a Low-Protein Diet. J. Agric. Food Chem. 2015 Sep 23 [PMID: 26366928] (WB, Rat)
• Wu L, Zhang Z, Pan X, Wang Z. Expression and contribution of the HIF1a/VEGF signaling pathway to luteal development and function in pregnant rats Mol Med Rep 2015 Aug 28 [PMID: 26323652] (Rat)
  
  Details:
  This citation used the HRP version of this antibody.
• Radulovic M, Yoon H, Wu J et al. Genetic Targeting of Protease Activated Receptor 2 Reduces Inflammatory Astrogliosis and Improves Recovery of Function after Spinal Cord Injury. Neurobiol. Dis. 2015 Aug 24 [PMID: 26316358] (WB, Mouse)
• Sato T, Ito Y, Nagasawa T. Attenuation of autophagic-proteolysis in C2C12 cells by saccharopine. Mol. Cell. Biochem. 2015 Aug 26 [PMID: 26307368] (WB, Mouse)
• Lin CL, Huang WN, Li HH et al. Hydrogen-rich water attenuates amyloid beta-induced cytotoxicity through upregulation of Sirt1-FoxO3a by stimulation of AMP-activated protein kinase in SK-N-MC cells. Chem. Biol. Interact. 2015 Aug 10 [PMID: 26271894] (WB, Human)
  
  Details:
  beta-Actin antibody was used for WB analysis of lysates from human neuroblastoma SK-N-MC cells that were subjected to treatments with Amyloid beta and / or hydrogen-rich water in vitro.
• Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015 Aug 03 [PMID: 26230691] (WB, Chicken)
  
  Details:
  beta-Actin antibody (clone AC-15) was used at 1:10,000 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
• Lim GE, Albrecht T, Piske M et al. 14-3-3zeta coordinates adipogenesis of visceral fat. Nat Commun 2015 [PMID: 26220403] (IP)
• Bamidele AO, Kremer KN, Hirsova P et al. IQGAP1 promotes CXCR4 chemokine receptor function and trafficking via EEA-1+ endosomes J. Cell Biol. 2015 Jul 20 [PMID: 26195666] (WB, Human)
• Chia-Wei Lin, Ming-Shiou Jan, Jung-Hua Steven Kuo. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. 2015 Aug 3 [PMID: 26158199]
• Yang C, Fischer-Keso R, Schlechter T et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression Tumour Biol. 2015 Jul 04 [PMID: 26138584] (WB, Human)
• Sasaki Y, Ohta M, Desai D et al. Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance PLoS ONE. 2015 Jul 03 [PMID: 26132105] (WB, Human, Mouse)
• Yang JS, Lin CW, Chuang CY et al. Carbonic anhydrase IX overexpression regulates the migration and progression in oral squamous cell carcinoma Tumour Biol. 2015 Jul 01 [PMID: 26130414] (WB, Human)
• Smith BW, Simpson DG, Miller RJ et al. Contrast Ultrasound Imaging Does Not Affect Heat Shock Protein 70 Expression in Cholesterol-Fed Rabbit Aorta J Ultrasound Med. 2015 Jul 01 [PMID: 26112623] (WB, Rabbit)
• Miki Y, Akimoto J, Moritake K et al. Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells Lasers Med Sci. 2015 Jun 25 [PMID: 26109138] (WB, Human)
• Chang CJ, Lin CS, Lu CC et al. Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun 2015 [PMID: 26102296] (Mouse)
• Xu Y, Zalzala M, Xu J et al. A metabolic stress-inducible miR-34a-HNF4a pathway regulates lipid and lipoprotein metabolism Nat Commun 2015 Jun 23 [PMID: 26100857] (WB, Mouse)
• Billeter AT, Galbraith N, Walker S et al. TRPA1 mediates the effects of hypothermia on the monocyte inflammatory response Surgery. 2015 Jun 05 [PMID: 26054320] (WB, Human)
• Viale-Bouroncle S, Klingelhoffer C, Ettl T, Morsczeck C. The AKT signaling pathway sustains the osteogenic differentiation in human dental follicle cells. Mol. Cell. Biochem. 2015 May 16 [PMID: 25981531] (WB, Human)
• Zhang Z, Pang X, Tang Z et al. Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015 Sep [PMID: 25975603]
  
  Details:
  This publication used the HRP conjugated form of this antibody (NB600-501H)
• Li HH, Lu FJ, Hung HC et al. Humic Acid Increases Amyloid beta-Induced Cytotoxicity by Induction of ER Stress in Human SK-N-MC Neuronal Cells.. Int J Mol Sci. 2015 May 7 [PMID: 25961951]
• Doriguzzi A, Haigl B, Gsur A, Sutterluty-Fall H. The increased Sprouty4 expression in response to serum is transcriptionally controlled by Specific protein 1. Int. J. Biochem. Cell Biol. 2015 May 06 [PMID: 25957915] (WB, Human)
• Wang ML, Lin SH, Hou YY, Chen YH. alpha-Naphthoflavone Increases Lipid Accumulation in Mature Adipocytes and Enhances Adipocyte-Stimulated Endothelial Tube Formation. Nutrients. 2015 May 06 [PMID: 25942489] (WB, Human)
• Yen JH, Lin LC, Chen MC, Sarang Z. The metastatic tumor antigen 1-transglutaminase-2 pathway is involved in selflimitationof monosodiumurate crystal-induced inflammation byupregulating TGF-beta1 Arthritis Research & Therapy 2015 Feb 26 [PMID: 25889736] (WB, Mouse, Human)
• Kao YT, Chang BL, Liang JJ et al. Japanese Encephalitis Virus Nonstructural Protein NS5 Interacts with Mitochondrial Trifunctional Protein and Impairs Fatty Acid beta-Oxidation PLoS Pathog. 2015 Mar 01 [PMID: 25816318] (WB, Human)
• Bonora M, Wieckowsk MR, Chinopoulos C et al. Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition. Oncogene 2015 Mar 19 [PMID: 25790189]
• Matteucci C, Minutolo A, Marino-Merlo F et al. Characterization of the enhanced apoptotic response to azidothymidine by pharmacological inhibition of NF-kB Life Sci. 2015 Mar 02 [PMID: 25744407] (WB, Human)
  
  Details:
  Beta-actin antibody was used as loading control for WB application in exeperiments involving AZT-induced NF-kB activation and its suppression by combined treatment with Bay-11-7085 in U937 cells (Fig. 2A).
• Wen J, Hanna CW, Martell S et al. Functional consequences of copy number variants in miscarriage Mol Cytogenet. 2015 Feb 12 [PMID: 25674159] (WB, Human)
• Capo-Chichi JM, Boissel S, Brustein E et al. Disruption of CLPB is associated with congenital microcephaly, severe encephalopathy and 3-methylglutaconic aciduria J. Med. Genet. 2015 Feb 03 [PMID: 25650066] (WB, Human, Zebrafish)
• Yoon H, Radulovic M, Drucker KL et al. The thrombin receptor is a critical extracellular switch controlling myelination Glia. 2015 Jan 27 [PMID: 25628003] (WB, Mouse)
• Chong KY, Hsu CJ, Hung TH et al. Wnt pathway activation and ABCB1 expression account for attenuation of Proteasome Inhibitor-mediated apoptosis in multidrug-resistant cancer cells Cancer Biol. Ther. 2015 Jan 15 [PMID: 25590413] (WB)
• Peng CC, Lin YT, Chen KC et al. Antrodan, A beta-glucan obtained from Antrodia cinnamomea mycelia, is beneficial to benign prostate hyperplasia.. Food Funct. 2015 Feb 11 [PMID: 25537160]
• Taher Chato, Frisk Gabriella, Fuentes Stina et al. High prevalence of human cytomegalovirus in brain metastases of patients with primary breast and colorectal cancers. Transl Oncol. 2014 Dec 01 [PMID: 25500083]
• Lin Sp, Chiu Fy, Wang Y et al. RB Maintains Quiescence and Prevents Premature Senescence through Upregulation of DnMT1 in Mesenchymal Stromal Cells. Stem Cell Reports. 2014 Nov 06 [PMID: 25455074] (WB, Human)
• Hsin Ch, Chen Mk, Tang Ch et al. High level of plasma matrix metalloproteinase-11 is associated with clinicopathological characteristics in patients with oral squamous cell carcinoma. PLoS OnE. 2014 Nov 26 [PMID: 25423087] (WB, Human)
• Pan Wr, Chen Yl, Hsu Hc,Chen Wj. Antimicrobial peptide GW-H1-induced apoptosis of human gastric cancer AGS cell line is enhanced by suppression of autophagy. Mol. Cell. Biochem. 2014 Nov 08 [PMID: 25380626] (WB, Human)
• Liu Y, Shim E, Crespo-Mejias Y et al. Cardiomyocytes are Protected from Antiretroviral nucleoside Analog-Induced Mitochondrial Toxicity by Overexpression of PGC-1Alpha Cardiovasc. Toxicol. 2014 Nov 07 [PMID: 25377427] (WB, Rat)
  
  Details:
  Beta actin antibody used for WB in experiments involving H9c2 cell line (derived from embryonic rat heart tissue).
• Chan Mt, Lim Ge, Skovso S et al. Effects of insulin on human pancreatic cancer progression modeled in vitro BMC Cancer et al. 2014 Nov 14 [PMID: 25373319] (WB, Human)
  
  Details:
  Beta actin antibody used as loading control for WB in experiments involving human primary pancreatic ductal cells, human HPDE cells and human PAnC1 cells.
• Lajud Sa, Nagda Da, Yamashita T et al. Dual Disruption of DnA Repair and Telomere Maintenance for the Treatment of Head and neck Cancer. Clin. Cancer Res. 2014 Oct 16 [PMID: 25324139] (WB, Human)
• Kokontis JM, Lin HP, Jiang SS et al. Androgen Suppresses the Proliferation of Androgen Receptor-Positive Castration-Resistant Prostate Cancer Cells via Inhibition of Cdk2, CyclinA, and Skp2. PLoS ONE. 2014 Oct 02 [PMID: 25271736] (WB, Human)
• Fischer-Keso R, Breuninger S, Hofmann S et al. Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins. Mol Cell Biol. 2014 Sep 15 [PMID: 25225333]
• Naik E, Webster JD, DeVoss J et al. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp. Med. 2014 Sep 22 [PMID: 25200027] (WB, Mouse)
• Qin Q, Xie H, Wise SS et al. Homologous Recombination Repair Signaling in Chemical Carcinogenesis: Prolonged Particulate Hexavalent Chromium Exposure Suppresses the Rad51 Response in Human Lung Cells. Toxicol. Sci. 2014 Aug 30 [PMID: 25173789] (WB, Human)
• Yang NC, Song TY, Chang YZ et al. Up-regulation of nicotinamide phosphoribosyltransferase and increase of NAD+ levels by glucose restriction extend replicative lifespan of human fibroblast Hs68 cells.. Biogerontology. 2015 Feb [PMID: 25146190]
• Hung YW, Hung SW, Wu YC et al. Soluble epoxide hydrolase activity regulates inflammatory responses and seizure generation in two mouse models of temporal lobe epilepsy Brain Behav. Immun. 2014 Aug 15 [PMID: 25135858]
• Dill-McFarland KA, Neil KL, Zeng A et al. Hibernation alters the diversity and composition of mucosa-associated bacteria while enhancing antimicrobial defense in the gut of 13-lined ground squirrels. Mol. Ecol. 2014 Aug 12 [PMID: 25130694] (WB, Mammal)
• Di K, Keir ST, Alexandru-Abrams D et al. Profiling Hsp90 differential expression and the molecular effects of the Hsp90 inhibitor IPI-504 in high-grade glioma models J. Neurooncol. 2014 Aug 13 [PMID: 25115740]
• Benayoun BA, Pollina EA, Ucar D et al. H3K4me3 Breadth Is Linked to Cell Identity and Transcriptional Consistency Cell 2014 Jul 31 [PMID: 25083876]
• Lin CW, Jan MS, Kuo JH. Autophagy-related gene expression analysis of wild-type and atg5 gene knockout mouse embryonic fibroblast cells treated with polyethylenimine. Mol Pharm. 2014 Sep 2 [PMID: 25075440]
• Bohr M, Brooks AR, Kurtz CC. Hibernation induces immune changes in the lung of 13-lined ground squirrels (Ictidomys tridecemlineatus). Dev. Comp. Immunol. 2014 Jul 18 [PMID: 25046152] (WB, Squirrel)
  
  Details:
  Beta Actin antibody used as loading control for WB on lung of 13-lined ground squirrels (Ictidomys tridecemlineatus).
• Vande Walle Lieselotte, Van Opdenbosch Nina, Jacques Peggy et al. Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis. Nature 2014 Aug 07 [PMID: 25043000] (WB, Mouse)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Chen Chin-Chen, Lin Jiun-Tsai, Cheng Yi-Fang et al. Amelioration of LPS-induced inflammation response in microglia by AMPK activation. Biomed Res Int. 2014 Jun 17 [PMID: 25025067]
• Wu CM, Lin KW, Teng CH et al. Chalcone derivatives inhibit human platelet aggregation and inhibit growth in human bladder cancer cells. Biol. Pharm. Bull. 2014 Jul 03 [PMID: 24989010] (WB, Human)
  
  Details:
  Beta actin antibody used as a loading control in WB application in lysates of human bladder cancer cells NTUB1.
• Lin KW, Huang AM, Lin CC et al. Anti-cancer effects of ursane triterpenoid as a single agent and in combination with cisplatin in bladder cancer Eur. J. Pharmacol. 2014 Jun 14 [PMID: 24933647] (WB, Human)
  
  Details:
  Beta Actin antibody used as loading control in WB assays on NTUB1 (a human bladder carcinoma cell) treated or not with Ursolic acid derivative isopropyl 3beta-hydroxyurs-12-en-28-oat (UA17) and cisplatin combinations. WB Images - Fig. 3C, Fig. 10 B and C.
• Chung PJ, Chi LM, Chen CL et al. microRNA-205 targets tight junction-related proteins during urothelial cellular differentiation. Mol. Cell Proteomics. 2014 Jun 09 [PMID: 24912853] (WB, Mouse)
  
  Details:
  Beta Actin antibody used for WB in 550ug lysate of normal urothelial cells (isolated from mouse bladder) treated with the antogomir against miR-205 (antagomiR-205 or Ant-205) or scramble control.
• Rahtu-Korpela Lea, Karsikas Sara, Horkko Sohvi et al. HIF prolyl 4-hydroxylase-2 inhibition improves glucose and lipid metabolism and protects against obesity and metabolic dysfunction. Diabetes. 2014 May 01 [PMID: 24789921] (Mouse)
• Press T, Viale-Bouroncle S, Felthaus O et al. EGR1 supports the osteogenic differentiation of dental stem cells. Int Endod J 2014 Apr 21 [PMID: 24749562] (WB, Human)
• Zeng W, Guo YH, Qi W et al. 4-Phenylbutyric acid suppresses inflammation through regulation of endoplasmic reticulum stress of endothelial cells stimulated by uremic serum. Life Sci. 3/17/2014 [PMID: 24650493] (WB, Human)
• Brugger D, Buffler M, Windisch W. Development of an experimental model to assess the bioavailability of zinc in practical piglet diets. Arch Anim Nutr 2014 Apr 1 [PMID: 24646148] (WB, Porcine)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Wong TW, Doyle AD, Lee JJ, Jelinek DF. Eosinophils Regulate Peripheral B Cell Numbers in Both Mice and Humans. J. Immunol. 3/10/2014 [PMID: 24616476] (WB, Human)
• Cai B, Xie S, Liu F et al. Rapid Degradation of the Complement Regulator, CD59, by a Novel Inhibitor. J. Biol. Chem. 3/10/2014 [PMID: 24616098] (WB, Human)
• Liu Y, Shim E, Nguyen P et al. Tempol Protects Cardiomyocytes from Nucleoside Reverse Transcriptase Inhibitor-Induced Mitochondrial Toxicity. Toxicol. Sci. 3/10/2014 [PMID: 24591154] (WB, Rat)
• Hung TM, Ho CM, Liu YC et al. Up-Regulation of MicroRNA-190b Plays a Role for Decreased IGF-1 That Induces Insulin Resistance in Human Hepatocellular Carcinoma. PLoS ONE 2014 Mar 3 [PMID: 24586785] (WB, Human)
• Zheng Xiaoxu, Heaps Cristine L, Fisher Steven A. Myosin phosphatase isoforms and related transcripts in the pig coronary circulation and effects of exercise and chronic occlusion. Microvasc Res. 2014 [PMID: 24534069] (Bovine)
• Van Opdenbosch Nina, Gurung Prajwal, Vande Walle Lieselotte et al. Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation. Nat Commun 2014 [PMID: 24492532] (WB)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Liang JJ, Wei JC, Lee YL et al. Surfactant-modified nanoclay exhibits an antiviral activity with high potency and broad spectrum. J. Virol. 2014 Jan 30 [PMID: 24478435] (WB, Human, Hamster)
• Yu CH, Yu CC. Photodynamic therapy with 5-aminolevulinic acid (ALA) impairs tumor initiating and chemo-resistance property in head and neck cancer-derived cancer stem cells. PLoS ONE 2014 Jan 29 [PMID: 24475244] (WB, Human)
• D'souza AM, Asadi A, Johnson JD, Kieffer TJ. Leptin Deficiency in Rats Results in Hyperinsulinemia and Impaired Glucose Homeostasis. Endocrinology 2014 Jan 28 [PMID: 24467741] (WB, Rat)
• Chung N, Chen Y, Yang S, et al. Induction of Th1/Th2-Balanced Protection Against SARS-CoV-2 Through Mucosal Delivery of An Adenovirus Vaccine Expressing an Engineered Spike Protein Research Square Jan 15 2021 (WB)
• Lin HP, Lin CY, Hsiao PH et al. Difference in Protein Expression Profile and Chemotherapy Drugs Response of Different Progression Stages of LNCaP Sublines and Other Human Prostate Cancer Cells. PLoS One 2013 Dec 5 [PMID: 24349321] (WB, Human)
• Rudalska R, Harbig J, Snaebjornsson M et al. LXR alpha activation and Raf inhibition trigger lethal lipotoxicity in liver cancer Nature Cancer Feb 1 2021
• Wang Y, Liao J, Chen K Et Al. Lumbrokinase regulates endoplasmic reticulum stress through IRE1 signaling to improve neurological deficits in ischemic stroke Research Square Jul 8 2021
• Kaczmarek MZ, Holland RJ, Lavanier SA et al. Mechanism of action for the cytotoxic effects of the nitric oxide prodrug JS-K in murine erythroleukemia cells. Leuk Res 2013 Dec 12 [PMID: 24461365] (WB, Mouse)
• Li KW, Yu YP, Zhou C et al. Calcium channel alpha-2-delta1 proteins mediate trigeminal neuropathic pain states associated with aberrant excitatory synaptogenesis. J. Biol. Chem. 2014 Jan 24 [PMID: 24459143] (WB, Rat)
• Zhu M, Guo M, Fei L et al. 4-Phenylbutyric acid attenuates endoplasmic reticulum stress-mediated pancreatic beta-cell apoptosis in rats with streptozotocin-induced diabetes. Endocrine 2013 Dec 18 [PMID: 24347242] (WB, Rat)
• Mounce BC, Mboko WP, Kanack AJ et al. Primary macrophages rely on histone deacetylase 1 and 2 expression to induce type I interferon in response to gammaherpesvirus infection. J. Virol. 2014 Feb 1 [PMID: 24335310] (WB, Mouse)
• Viale-Bouroncle S, Gosau M, Morsczeck C. NOTCH1 signaling regulates the BMP2/DLX-3 directed osteogenic differentiation of dental follicle cells. Biochem Biophys Res Commun 2014 Jan 10 [PMID: 24321094] (WB, Human)
• Schusser B, Yi H, Collarini EJ et al. Harnessing Gene Conversion in Chicken B Cells to Create a Human Antibody Sequence Repertoire. PLoS One. 2013 Nov 21 [PMID: 24278246] (WB, Chicken)
• Govaert KM, Emmink BL, Nijkamp MW et al. Hypoxia After Liver Surgery Imposes an Aggressive Cancer Stem Cell Phenotype on Residual Tumor Cells. Ann Surg 2013 Nov 18 [PMID: 24253142] (WB, Mouse)
• Allache R, Lachance S, Guyot MC et al. Novel mutations in Lrp6 orthologs in mouse and human neural tube defects affect a highly dosage-sensitive Wnt non-canonical planar cell polarity pathway. Hum Mol Genet. 2013 Nov 18 [PMID: 24203697] (WB, Human)
• Zhou C, Luo ZD. Electrophysiological characterization of spinal neuron sensitization by elevated calcium channel alpha-2-delta-1 subunit protein. Eur J Pain. 2013 Oct 23 [PMID: 24151064]
• Thies HW, Nolte I, Wenk H et al. Permanent activation of HMGA2 in lipomas mimics its temporal physiological activation linked to the gain of adipose tissue. Obesity (Silver Spring). 2012 Nov 6 [PMID: 24106133] (WB, Canine, Human)
• Xu J, Li Y, Chen WD et al. Hepatic carboxylesterase 1 Is essential for both normal and farnesoid X receptor-controlled lipid homeostasis. Hepatology. 2013 Aug 28 [PMID: 24038130] (WB, Mouse)
• Gini B, Zanca C, Guo D et al. The mTOR kinase inhibitors, CC214-1 and CC214-2, preferentially block the growth of EGFRvIII-activated glioblastomas. Clin Cancer Res 2013 Oct 15 [PMID: 24030701]
• Li KW, Kim DS, Zaucke F et al. Trigeminal nerve injury-induced thrombospondin-4 up-regulation contributes to orofacial neuropathic pain states in a rat model. Eur J Pain. 2013 Sep 9 [PMID: 24019258]
• Snoeren N, Emmink BL, Koerkamp MJ et al. Maspin is a marker for early recurrence in primary stage III and IV colorectal cancer. Br J Cancer. 2013 Sep 17 [PMID: 24002600] (WB, Human)
• Feldman AL, Vasmatzis G, Asmann YW et al. Novel TRAF1-ALK fusion identified by deep RNA sequencing of anaplastic large cell lymphoma. Genes Chromosomes Cancer. 2013 Sep 2 [PMID: 23999969] (WB, Human)
• Chan CC, Lee KC, Huang YH et al. Regulation by Resveratrol of the Cellular Factors Mediating Liver Damage and Regeneration after Acute Toxic Liver Injury. J Gastroenterol Hepatol. 2013 Aug 23 [PMID: 23981054] (WB, Mouse)
• Khan Z, Yaiw KC, Wilhelmi V et al. Human cytomegalovirus immediate early proteins promote degradation of connexin 43 and disrupt gap junction communication: implications for a role in gliomagenesis. Carcinogenesis 2014 Jan [PMID: 23978378]
• Liu L, Martin R, Kohler G, Chan C. Palmitate induces transcriptional regulation of BACE1 and presenilin by STAT3 in neurons mediated by astrocytes. Exp Neurol 2013 Oct [PMID: 23968646] (WB, Rat)
  
  Details:
  Using the HRP conjugated version of NB600-501, catalog number NB600-501H.
• Chang KP, Wang CL, Kao HK et al. Overexpression of caldesmon is associated with lymph node metastasis and poorer prognosis in patients with oral cavity squamous cell carcinoma. Cancer. 2013 Aug 20 [PMID: 23963810] (WB, Human)
• Hsu PK, Chen HY, Yeh YC et al. TPX2 expression is associated with cell proliferation and patient outcome in esophageal squamous cell carcinoma. J Gastroenterol. 2013 Aug 21 [PMID: 23963785] (WB, Human)
• Lee CC, Lai YT, Chang HT et al. Inhibition of high-mobility group box 1 in lung reduced airway inflammation and remodeling in a mouse model of chronic asthma. Biochem Pharmacol. 2013 Aug 12 [PMID: 23948063] (WB, Mouse)
• Yeh CN, Chiang KC, Juang HH et al. Reappraisal of the Therapeutic Role of Celecoxib in Cholangiocarcinoma. PLoS One 2013 Jul 26 [PMID: 23922859] (WB, Rat)
• Hsieh YT, Chou MM, Chen HC, Tseng JJ. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. Gynecol Oncol. 2013 Oct [PMID: 23911878]
• Morel E, Chamoun Z, Lasiecka ZM et al. Phosphatidylinositol-3-phosphate regulates sorting and processing of amyloid precursor protein through the endosomal system. Nat Commun 2013 Aug 2 [PMID: 23907271] (WB, Human)
• Tigrine C, Bulzomi P, Leone S et al. Cleome arabica leaf extract has anticancer properties in Human cancer cells. Pharm Biol 2013 Jul 18 [PMID: 23862683] (WB, Human)
• Wen J, Lopes F, Soares G et al. Phenotypic and functional consequences of haploinsufficiency of genes from exocyst and retinoic acid pathway due to a recurrent microdeletion of 2p13.2. Orphanet J Rare Dis 2013 Jul 10 [PMID: 23837398] (WB, Human)
• Emmink BL, Verheem A, Van Houdt WJ et al. The secretome of colon cancer stem cells contains drug-metabolizing enzymes. J Proteomics 2013 Jul 5 [PMID: 23835434] (WB, Human)
• Rathmanner N, Haigl B, Vanas V et al. Sprouty2 but not Sprouty4 is a potent inhibitor of cell proliferation and migration of osteosarcoma cells. FEBS Lett 2013 Jul 4 [PMID: 23831057] (WB, Human)
• Kremer KN, Peterson KL, Schneider PA et al. SDF-1/CXCR4 signaling induces apoptosis in acute myeloid leukemia cells via regulation of the Bcl-2 family members Bcl-XL, Noxa, and Bak. J Biol Chem 2013 Jun 24 [PMID: 23798675] (WB, Human)
• Ko CH, Cheng CF, Lai CP et al. Differential proteomic analysis of cancer stem cell properties in hepatocellular carcinomas by isobaric tag labeling and mass spectrometry. J Proteome Res 2013 Aug 2 [PMID: 23782096] (WB, Human)
• Shie MY, Ding SJ. Integrin binding and MAPK signal pathways in primary cell responses to surface chemistry of calcium silicate cements. Biomaterials 2013 Sep [PMID: 23768900] (ELISA, Human)
• Liu T, Zhang S, Xiang D, Wang Y. Induction of hepatocyte-like cells from mouse embryonic stem cells by lentivirus-mediated constitutive expression of Foxa2/Hnf4a. J Cell Biochem 2013 Jun 6 [PMID: 23744720] (WB, Mouse)
• Babic I, Anderson ES, Tanaka K et al. EGFR Mutation-Induced Alternative Splicing of Max Contributes to Growth of Glycolytic Tumors in Brain Cancer. Cell Metab 2013 Jun 4 [PMID: 23707073] (WB, Human)
• Formosa R, Xuereb-Anastasi A, Vassallo J. AIP regulates cAMP signalling and growth hormone secretion in GH3 cells. Endocr Relat Cancer 2013 May 23 [PMID: 23702468] (WB, ICC/IF, Rat)
• Das I, Png CW, Oancea I et al. Glucocorticoids alleviate intestinal ER stress by enhancing protein folding and degradation of misfolded proteins. J Exp Med 2013 May 6 [PMID: 23650437] (WB, Human)
• Zeng J, Kim D, Li KW et al. Thrombospondin-4 contributes to spinal cord injury-induced changes in nociception. Eur J Pain 2013 May 7 [PMID: 23649982]
• Yoon H, Radulovic M, Wu J et al. Kallikrein 6 signals through PAR1 and PAR2 to promote neuron injury and exacerbate glutamate neurotoxicity. J Neurochem. 2013 Oct [PMID: 23647384] (WB, IP, Mouse)
• Rafalski VA, Ho PP, Brett JO et al. Expansion of oligodendrocyte progenitor cells following SIRT1 inactivation in the adult brain.. Nat Cell Biol 2013 Jun [PMID: 23644469] (WB, Mouse)
• Cai H, Peng F. Knockdown of copper chaperone antioxidant-1 by RNA interference inhibits copper-stimulated proliferation of non-small cell lung carcinoma cells. Oncol Rep 2013 Jul [PMID: 23624903]
• Kral RM, Mayer CE, Vanas V et al. In non-small cell lung cancer mitogenic signaling leaves Sprouty1 protein levels unaffected. Cell Biochem Funct 2013 Apr 24 [PMID: 23616430] (WB, Human)
• Huang TT, Chong KY, Ojcius DM et al. Hirsutella sinensis mycelium suppresses interleukin-1beta and interleukin-18 secretion by inhibiting both canonical and non-canonical inflammasomes. Sci Rep 2013 Mar 5 [PMID: 23459183] (WB, Human)
• Park Y, Carr TPet al. Unsaturated fatty acids and phytosterols regulate cholesterol transporter genes in Caco-2 and HepG2 cell lines. Nutr Res. 2013 Feb [PMID: 23399666] (WB, Human)
• Allen JJ, Bell GR, Kuzirian AM, Hanlon RT. Cuttlefish skin papilla morphology suggests a muscular hydrostatic function for rapid changeability. J Morphol 2013 Feb 2 [PMID: 23378271] (IHC, ICC/IF, Fish)
• Drucker KL, Paulsen AR, Giannini C et al. Clinical significance and novel mechanism of action of kallikrein 6 in glioblastoma Neuro Oncol 2013 Jan 10 [PMID: 23307575] (WB, Human)
• Salih DA, Rashid AJ, Colas D et al. FoxO6 regulates memory consolidation and synaptic function Genes Dev 2012 Dec 15 [PMID: 23222102] (WB, Mouse)
• Shih H J, Chen H H et al. Targeting MCT-1 oncogene inhibits Shc pathway and xenograft tumorigenicity. Oncotarget Jan 11 2012 [PMID: 23211466] (WB, Human)
• Berryer MH, Hamdan FF, Klitten LL et al. Mutations in SYNGAP1 Cause Intellectual Disability, Autism, and a Specific Form of Epilepsy by Inducing Haploinsufficiency Hum Mutat 2012 Nov 15 [PMID: 23161826] (WB, Human)
• Xie W, Parker JL, Heaps CL. Exercise Training-Enhanced, Endothelium-Dependent Dilation Mediated by Altered Regulation of BK(Ca) Channels in Collateral-Dependent Porcine Coronary Arterioles. Microcirculation 2013 Feb [PMID: 23002811]
• Lin CW, Jan MS, Kuo JH et al. Protective role of autophagy in branched polyethylenimine (25K)- and poly(L-lysine) (30-70K)-induced cell death Eur J Pharm Sci 2012 Sep 21 [PMID: 23000135] (WB, Human)
• Hanell A, Clausen F, Djupsjo A et al. Functional and Histological Outcome after Focal Traumatic Brain Injury Is Not Improved in Conditional EphA4 Knockout Mice J Neurotrauma 2012 Nov 1. [PMID: 22985250]
• Lee WC, Kan D, Chen YY et al. Suppression of Extensive Neurofilament Phosphorylation Rescues alpha-Internexin/Peripherin-Overexpressing PC12 Cells from Neuronal Cell Death PLoS One 2012 [PMID: 22952800] (WB, Rat)
• Aro E, Khatri R, Gerard-O'Riley R et al. Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes J Biol Chem 2012 Aug 28 [PMID: 22930750] (WB, Mouse)
• Maj M, Milenkovic I, Bauer J et al. Novel insights into the distribution and functional aspects of the calcium binding protein secretagogin from studies on rat brain and primary neuronal cell culture Front Mol Neurosci 2012 [PMID: 22888312] (WB, Human, Rat)
• Minutolo A, Grelli S, Marino-Merlo F et al. D(-)lentiginosine-induced apoptosis involves the intrinsic pathway and is p53-independent Cell Death Dis 2012 Jul 26 [PMID: 22833097] (WB, Human)
• Calnan DR, Webb AE, White JL et al. Methylation by Set9 modulates FoxO3 stability and transcriptional activity Aging 2012 Jul [PMID: 22820736] (WB, Human)
• Wu H, Lu Y, Shen C et al. Distinct Roles of Muscle and Motoneuron LRP4 in Neuromuscular Junction Formation Neuron 2012 Jul 12 [PMID: 22794264] (WB, Mouse)
• Wu MR, Hou MH, Lin YL, Kuo CF. 2,4,5-TMBA, a natural inhibitor of cyclooxygenase-2, suppresses adipogenesis and promotes lipolysis in 3T3-L1 adipocytes.. J Agric Food Chem. 2012 Jul 25 [PMID: 22746947]
• Li T, Liu G, Li J, Wang X, Liu Q, Liu Z, Du W. Mechanisms of Prostate Permeability Triggered by Microbubble-Mediated Acoustic Cavitation Cell Biochem Biophys 2012 Jun 22 [PMID: 22722876] (WB, Rabbit)
• Wehenkel M, Ban JO, Ho YK et al. A selective inhibitor of the immunoproteasome subunit LMP2 induces apoptosis in PC-3 cells and suppresses tumour growth in nude mice Br J Cancer 2012 Jun 26 [PMID: 22677907] (WB, Human)
• Dawson PA, Rakoczy J, Simmons DG. Placental, Renal, and Ileal Sulfate Transporter Gene Expression in Mouse Gestation Biol Reprod 2012 Aug 23 [PMID: 22674389] (WB, Mouse)
• Wu H, Lu Y, Barik A et al. Beta-Catenin gain of function in muscles impairs neuromuscular junction formation Development 2012 Jul [PMID: 22627288] (WB, Mouse)
• Herroon MK, Rajagurubandara E, Rudy DL, Chalasani A, Hardaway AL, Podgorski I. Macrophage cathepsin K promotes prostate tumor progression in bone. Oncogene. 2012 May 21. [PMID: 22614014] (WB, Human)
• Pohjanvirta R, Miettinen H, Sankari S, Hegde N, Linden J. Unexpected gender difference in sensitivity to the acute toxicity of dioxin in mice. Toxicol Appl Pharmacol. 2012 May 4. [PMID: 22564538] (WB, Mouse)
• Yu M, Ge C, Zeng W et al. Retinoic acid promotes proliferation of chicken primordial germ cells via activation of PI3K/Akt-mediated NF-kappa B signalling cascade Cell Biol Int 2012 Aug 1 [PMID: 22548360] (WB, Chicken)
• Chang WW, Hu FW, Yu CC et al. Quercetin in elimination of tumor initiating stem-like and mesenchymal transformation property in head and neck cancer Head Neck 2013 Mar [PMID: 22422628] (WB, Human)
• Hsieh YL, Chou LW, Chang PL, Yang CC, Kao MJ, Hong CZ. Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury-possible involvements in hypoxia-inducible factor 1? (HIF-1?). J Comp Neurol doi: 10.1002/cne.23072. 2012 Feb 20. [PMID: 22351621]
• Carmony KC, Kim KB. PROTAC-induced proteolytic targeting. Methods Mol Biol 2012 [PMID: 22350917] (Human)
• Govaert KM, Nijkamp MW, Emmink BL, Steller EJ, Minchinton AI, Kranenburg O, Borel Rinkes IH. Effects of tirapazamine on experimental colorectal liver metastases after radiofrequency ablation. Br J Surgr;99(4):567-75. doi: 10.1002/bjs.8668. 2012 Apr. [PMID: 22331808]
• Zhang B, Liang C, Bates R et al. Wnt proteins regulate acetylcholine receptor clustering in muscle cells Mol Brain 2012 Feb 6 [PMID: 22309736] (WB, Mouse)
• Zhou Q, Xu G, Zhang X, Cao C, Zhou Z. Proteomics of post-traumatic proliferative vitreoretinopathy in rabbit retina reveals alterations to a variety of functional proteins. Curr Eye Res37(4):318-26. 2012 Apr. [PMID: 22295879] (WB, Rabbit)
• Swain U, Rao KS. Age-dependent decline of DNA base excision repair activity in rat cortical neurons. Mech Ageing Dev. 2012 Apr [PMID: 22266244] (WB, Rat)
• Lee Wen-Ching, Chen Yun-Yu, Kan Daphne et al. A neuronal death model: overexpression of neuronal intermediate filament protein peripherin in PC12 cells. Journal of Biomedical Science 2012 [PMID: 22252275] (WB, Mouse)
• Szabat M, Kalynyak TB, Lim GE et al. Musashi expression in [beta]-cells coordinates insulin expression, apoptosis and proliferation in response to endoplasmic reticulum stress in diabetes. Cell Death Dis 20112:e232. [PMID: 22113197]
• Patschan O, Shariat SF, Chade DC et al. Association of tumor-associated trypsin inhibitor (TATI) expression with molecular markers, pathologic features and clinical outcomes of urothelial carcinoma of the urinary bladder World J Urol 2012 Dec [PMID: 21739120] (IHC, Human)
• Corcoran MP, Lichtenstein AH, Meydani M et al. The effect of 17{beta}-estradiol on cholesterol content in human macrophages is influenced by the lipoprotein milieu. J Mol Endocrinol. 2011 Jun 6. [PMID: 21646386] (WB, Human)
• Hamel KM The Age-Dependent Characterization of the ER-Alpha Positive Breast Cancer Tumor Microenvironment Thesis Jan 1 2021
• Edgel KA, McMillen TS, Wei H et al. Obesity and weight loss result in increased adipose tissue ABCG1 expression in db/db mice. Biochim Biophys Acta 2012 Mar [PMID: 22179025] (WB, Mouse)
• Tsai YY, Huang YH, Chao YL, Hu KY, Chin LT, Chou SH, Hour AL, Yao YD, Tu CS, Liang YJ, Tsai CY, Wu HY, Tan SW, Chen HM. Identification of the nanogold particle-induced endoplasmic reticulum stress by omic techniques and systems biology analysis. ACS Nano;5(12):9354-69. 2011 Dec 27. [PMID: 22107733] (WB, Human)
• Yang L-X, Yang Z-H, Guo R-W et al. Angiotensin II induces extracellular matrix metalloproteinase inducer expression via an AT1R dependent pathway in aortic atherosclerotic plaque in apolipoprotein E knockout mice. J Renin Angiotensin Aldosterone Syst. 2011 Oct 21. [PMID: 22020146]
• Khan Z, Akhtar M, Ekstrom TJ. HDAC inhibitor 4-phenylbutyrate preserves immature phenotype of human embryonic midbrain stem cells: implications for the involvement of DNA methyltransferase. Int J Mol Med 2011 Dec [PMID: 21894430] (WB, Human)
• Chiang K-C, Yeh C-N, Chen H-Y et al. 19-Nor-2[alpha]-(3-hydroxypropyl)-1[alpha],25-dihydroxyvitamin D(3) (MART-10) is a potent cell growth regulator with enhanced chemotherapeutic potency in liver cancer cells. Steroids. 2011 Aug 25. [PMID: 21888924]
• Gao L, Coope H, Grant S et al. ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling. J Biol Chem 2011 Oct 21 [PMID: 21885437]
• van Houdt W J, Emmink B L et al. Comparative proteomics of colon cancer stem cells and differentiated tumor cells identifies BIRC6 as a potential therapeutic target. Mol Cell Proteomics Jan 12 2011 [PMID: 21788403] (WB, Human)
• Ge X, Yin L, Ma H et al. Aldo-keto reductase 1B7 is a target gene of FXR and regulates lipid and glucose homeostasis. [PMID: 21642744]
• Vitari AC, Leong KG, Newton K et al. COP1 is a tumour suppressor that causes degradation of ETS transcription factors. Nature 2011 Jun 16 [PMID: 21572435]
• Alicea GM Metabolic Consequences of an Aging Microenvironment in Melanoma Thesis Jan 1 2020 (WB, Human, Mouse)
• Winter N, Nimzyk R, Bosche C et al. Chromatin immunoprecipitation to analyze DNA binding sites of HMGA2 PLoS One 2011 Apr 14 [PMID: 21533145] (WB, Human)
• Nijkamp MW, Hoogwater FJ, Govaert KM et al. A role for CD95 signaling in ischemia/reperfusion-induced invasion and outgrowth of colorectal micrometastases in mouse liver J Surg Oncol 2011 Aug 1 [PMID: 21506133] (WB, Mouse)
• Huang AM, Kao YT, Toh S et al. UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity Biochem Pharmacol 2011 Jul 1 [PMID: 21477582] (WB, Human)
• Raats DAE, de Bruijn MT, Steller EJA et al. Synergistic killing of colorectal cancer cells by oxaliplatin and ABT-737. Cell Oncol (Dordr);34(4):307-13. 2011 Aug. [PMID: 21468686]
• Scarisbrick IA, Epstein B, Cloud BA et al. Functional Role of Kallikrein 6 in Regulating Immune Cell Survival. PLoS ONE. 2011 Mar 28. [PMID: 21464892]
• Kim SJ, Nian C, McIntosh CH. Adipocyte expression of the glucose-dependent insulinotropic polypeptide receptor involves gene regulation by PPARgamma and histone acetylation. J Lipid Res 2011 Apr [PMID: 21245029] (WB, Mouse)
• HildebrJM, Luo Z, Manske MK et al. A BAFF-R mutation associated with non-Hodgkin lymphoma alters TRAF recruitment and reveals new insights into BAFF-R signaling. J Exp Med. 2010 Nov 1. [PMID: 21041452]
• Moiseyev, G et al Inhibition of the visual cycle by A2E through direct interaction with RPE65 and implications in Stargardt disease. Proc Natl Acad Sci U S A 2010 Oct 12 [PMID: 20876139]
  
  Details:
  Using the Purified form of this antibody.
• Chavez CL, Keravala A, Woodard LE et al. Kinetics and longevity of PhiC31 integrase in mouse liver and cultured cells. Hum Gene Ther 2010 Oct [PMID: 20497035] (WB)
• van Houdt WJ, de Bruijn MT, Emmink BL et al. Oncogenic K-ras activates p38 to maintain colorectal cancer cell proliferation during MEK inhibition. Cell Oncol 2010 Jan 1 [PMID: 20413844]
• Smith B. Effects of nutrition and ultrasound imaging on the cardiovascular system Thesis. 2014 (WB, Rabbit)
• Chen CC, Chyau CC, Liao CC et al. Enhanced anti-inflammatory activities of Monascus pilosus fermented products by addition of ginger to the medium.. J Agric Food Chem. 2010 Nov 24 [PMID: 20958048]
• Chu D, Nguyen A, Smith S et al. Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development bioRxiv Jun 23 2020 (WB, Chicken)
• de Bruijn MT, Raats DA, Hoogwater FJ et al. Oncogenic KRAS sensitises colorectal tumour cells to chemotherapy by p53-dependent induction of Noxa. Br J Cancer 2010 Apr 13 [PMID: 20354524] (WB, Human)
• Galasso C. Marine Biotechnology: a sea of new resources and solutions for ocean and human health. Thesis. Jul 9 1905 (Human)
• Lan CH, Sheng JQ, Fang DC et al. Involvement of VDAC1 and Bcl-2 family of proteins in VacA-induced cytochrome c release and apoptosis of gastric epithelial carcinoma cells. J Dig Dis 2010 Feb [PMID: 20132430] (WB, Human)
• Carmony KC. Elucidating Proteasome Catalytic Subunit Composition and Its Role in Proteasome Inhibitor Resistance. Thesis. Apr 18 2016 (WB, Human)
• Boothe T, Lim GE, Cen H et al. Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells Molecular Metabolism Feb 10 2016 (WB, Mouse)
• Ortolan L, Sercundes M, Moura G et al. Critical contribution of endothelial protein C receptor in experimental malaria-associated acute respiratory distress syndrome bioRxiv Jun 15 2018 (WB, Mouse)
• Chia-Feng Kuo, Chih-Hsuan Hsieh, Wun-Yuan Lin. Proteomic response of LAP-activated RAW 264.7 macrophages to the anti-inflammatory property of fungal ergosterol.. Food chemistry. 2011
• Matluk N, Rochira JA, Karaczyn A et al. A role for NRAGE in NF-kappaB activation through the non-canonical BMP pathway. BMC Biol 2010 [PMID: 20100315] (WB)
• Mahmoudi S, Mancini E, Moore A et al. Old fibroblasts secrete inflammatory cytokines that drive variability in reprogramming efficiency and may affect wound healing between old individuals bioRxiv Oct 20 2018 (WB, Mouse, Human)
• M Maj, W Gartner, A Ilhan et al. Expression of TAU in insulin-secreting cells and its interaction with the calcium-binding protein secretagogin. J. Endocrinol. 205 (1): 25-36. 2010 [PMID: 20061514]
• Yang S, Chen Z, Fan D et al. MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3 Am. J. Physiol. Renal Physiol. Oct 10 2018 (WB, Human)
• Walle LV, Stowe IB, Sacha P MCC950/CRID3 potently targets the NACHT domain of wildtype NLRP3 but not disease-associated mutants for inflammasome inhibition bioRxiv May 11 2019 (WB, Mouse)
• Zhang B, Luo S, Wang Q et al. LRP4 serves as a coreceptor of agrin. Neuron 2008 Oct 23 [PMID: 18957220] (WB)
• Luo S, Zhang B, Dong XP et al. HSP90 beta regulates rapsyn turnover and subsequent AChR cluster formation and maintenance. Neuron 2008 Oct 9 [PMID: 18940591] (Mouse)
• Fogarty JA, Delp MD, Muller-Delp JM et al. Neuropilin-1 is essential for enhanced VEGF(165)-mediated vasodilatation in collateral-dependent coronary arterioles of exercise-trained pigs. J Vasc Res 2009 [PMID: 18769069] (WB)
• Elsawa Sherine F, Novak Anne J, Grote Deanna et al. CDDO-imidazolide mediated inhibition of malignant cell growth in Waldenstrom macroglobulinemia. Leukemia Research 2008 [PMID: 18468679] (WB, Human)
• Heazlewood, C et al. Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis. PLoS Med;5(3):e54. 2008 Mar 4. [PMID: 18318598]
• Carlet M, Voelse K, Vergalli J et al. In vivo inducible reverse genetics in patients' tumors to identify individual therapeutic targets bioRxiv Jan 1 2020 (WB, KD, Mouse)
• Dror V, Kalynyak TB, Bychkivska Y et al. Glucose and endoplasmic reticulum calcium channels regulate HIF-1beta via presenilin in pancreatic beta-cells. J Biol Chem;283(15):9909-16. 2008 Apr 11. [PMID: 18174159]
• Botezelli JD, Overby P, Lindo L et al. Adipocyte depot-specific up-regulation of uncoupling protein 1 or mitochondrial oxidative complex proteins are early consequences of genetic insulin reduction in mice bioRxiv Jan 1 2020 (WB, Mouse)
• Macchi B, Minutolo A, Grelli S et al. The novel proapoptotic activity of nonnatural enantiomer of Lentiginosine. Glycobiology 2010 May. [PMID: 20053629] (WB, Human)
• Woodard LE, Hillman RT, Keravala A et al. Effect of nuclear localization and hydrodynamic delivery-induced cell division on phiC31 integrase activity. Gene Ther 2010 Feb [PMID: 19847205] (WB, Human)
• Barthel SR, Gavino JD, Wiese GK et al. Analysis of glycosyltransferase expression in metastatic prostate cancer cells capable of rolling activity on microvascular endothelial (E)-selectin. Glycobiology;18(10):806-17. 2008 Oct. [PMID: 18647941]
• Alejandro EU, Johnson JD. Inhibition of Raf-1 alters multiple downstream pathways to induce pancreatic beta-cell apoptosis. J Biol Chem 283(4):2407-17. 2008 Jan 25. [PMID: 18006502]
• Rice, PL et al Rice PL, Peters SL, Beard KS, Ahnen DJ. Sulindac independently modulates extracellular signal-regulated kinase 1/2 and cyclic GMP-dependent protein kinase signaling pathways. Mol Cancer Ther 5(3):746-54. 2006 Mar. [PMID: 16546990]
• Csiszar, A et al. Bone morphogenetic protein-2 induces proinflammatory endothelial phenotype. Am J Pathol 168(2):629-38. 2006 Feb. [PMID: 16436676]
• Herroon MK, Diedrich JD, Rajagurubandara E Prostate Tumor Cell-Derived IL1b Induces an Inflammatory Phenotype in Bone Marrow Adipocytes and Reduces Sensitivity to Docetaxel via Lipolysis-Dependent Mechanisms researchgate.net (Mouse)
• Trent B, Liang Y, Xing Y et al. Polarized Lung Inflammation and Tie2/Angiopoietin-Mediated Endothelial Dysfunction during Severe Orientia tsutsugamushi Infection BioRxi 2019 Aug 2 (WB, Mouse, Human)
  
  Details:
  Citation using the HRP format of this antibody.
• Chowdhury, A Tamalin/GRAS-1 Connects Glutamate Receptor Activity to the Insulin/IGF Signaling Cascade to Regulate Neuroprotection in a Nematode Model of Excitotoxicity Thesis Jan 1 2020 (WB, C. elegans)
• Greer, EL et al. The energy sensor AMP-activated protein kinase directly regulates the mammalian FOXO3 transcription factor. J Biol Chem 282(41):30107-19. 2007 Oct 12. [PMID: 17711846]
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Reviews for beta-Actin Antibody (NB600-501) (26)

Average Rating: 4.8

(Based on 26 reviews)

We have 26 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Verified Customer WB Human 05/16/2021

Summary

• Application: Western Blot
• Sample Tested: HEK293
• Species: Human
• Lot: 90942

reviewed by: John Morgan WB Human 01/31/2019

Summary

• Application: Western Blot
• Sample Tested: HepG2 CELLS
• Species: Human
• Lot: 106M4825V-4

reviewed by: Anthony Morales WB Rat 01/28/2019

Summary

• Application: Western Blot
• Sample Tested: Mouse brain
• Species: Rat
• Lot: 014M4759

reviewed by: Verified Customer WB Human 01/24/2019

Summary

• Application: Western Blot
• Sample Tested: mouse hepatocytes and HepG2 cells
• Species: Human
• Lot: 105M4825V-3

reviewed by: Luiz Alexandre Magno WB Mouse 07/17/2018

Summary

• Application: Western Blot
• Sample Tested: Mouse brain,Brain (corpus striatum)
• Species: Mouse
• Lot: 014M4759

Comments

• Comments: I have used this antibody for several years and it has worked perfectly in our western blots. It works very well at 1:10,00 dilution when loading gels with 30 ug of protein extracts. I congratulate Novus Biologicals for this achievement.

reviewed by: Verified Customer WB Human 02/20/2018

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human
• Lot: 105M4825V-3

reviewed by: Liyong Zhang WB Human 11/25/2017

Summary

• Application: Western Blot
• Sample Tested: mouse hepatocytes and HepG2 cells
• Species: Human
• Lot: 105M4825V-3

reviewed by: Verified Customer WB Human 06/05/2015

Summary

• Application: Western Blot
• Sample Tested: human glioma cells
• Species: Human

Blocking

• Blocking Details: 10% milk in TBST

Details

• Detection Notes: ECL western blotting detection reagent

reviewed by: Verified Customer WB Human 05/29/2015

Summary

• Application: Western Blot
• Sample Tested: Fibroblast Hs68 cell lysate
• Species: Human

Blocking

• Blocking Details: See PMID: 25146190

Details

• Detection Notes: See PMID: 25146190

Comments

• Comments: Effects of glucose restriction (GR) on the expression of Nampt protein in Hs68 cells. beta-Actin used as a loading control. See PMID: 25146190 for details

reviewed by: Verified Customer WB Human 03/25/2015

Summary

• Application: Western Blot
• Sample Tested: Caco-2, T84, HCT116, HT29, DU145, BPH1, Hela
• Species: Human

Blocking

• Blocking Details: 1 hour at RT in 5% milk powder solution (in 1xTBST)

Primary Anitbody

• Dilution Ratio: Dilution:1/15000, 1 hour at RT

Secondary Antibody

• Secondary Description: HRP

Details

• Detection Notes: ECL detection

reviewed by: Verified Customer WB Other 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: Whole Skeletal Muscle Lysate
• Species: Other

Blocking

• Blocking Details: 3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

• Dilution Ratio: 1/4000 at 4C for 18 hr

Secondary Antibody

• Secondary Description: Goat anti-Mouse 680 RD - Licor Biosciences
• Secondary Manufacturer Cat#: 925-68070
• Secondary Concentration: 1/20,000

Details

• Detection Notes: Licor Odyssey

reviewed by: Sandra Vieira WB Human 01/23/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates: CHO, GC-1, Hela, Cos-7, SH-SY5Y
• Species: Human
• Lot: 083M4851

Blocking

• Blocking Details: blocking solution was added for 1h (5% non-fat dry milk in 1x TBS-T)

Primary Anitbody

• Dilution Ratio: Primary antibody B-Actin (1:10000) , diluted in 3% non-fat dry milk in 1x TBS-T, was added and incubated with membranes 1h

Secondary Antibody

• Secondary Description: Goat anti-mouse
• Secondary Concentration: 1:5000

Details

• Detection Notes: the enhanced chemiluminescence (ECL) detection method; Homemade ECL 1 min; Exposure time: 5 min

reviewed by: Liyong Zhang WB Mouse 07/01/2014

Summary

• Application: Western Blot
• Species: Mouse
• File: View PDF

reviewed by: Beatrice Gini WB Human 12/02/2013

Summary

• Application: Western Blot
• Sample Tested: human brain cells
• Species: Human
• Comments: Clin Cancer Res. 2013 Oct 15;19(20):5722-32.

Blocking

• Blocking Details: 5% BSA-TBS 1X-Tween 0.1% in dH2O , 1h, RT

Primary Anitbody

• Dilution Ratio: 1:10000, 1 h, RT, in TBS 1X-Tween 0.1% in dH2O

Secondary Antibody

• Secondary Description: anti-mouse, 1:5000

Details

• Detection Notes: Thermo detection reagent

Comments

• Comments: Clin Cancer Res. 2013 Oct 15;19(20):5722-32.

reviewed by: Verified Customer WB Human 12/21/2011

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell line
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% FBS

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse

Details

• Detection Notes: Detection Method: ECL

reviewed by: Verified Customer WB Mouse 12/21/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse BALB/c macrophage cell line
• Species: Mouse
• Comments: The cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat milk in PBST, Blocking Time: 1 hour

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: Goat anti-Mouse IgG (HRP), Jackson

Details

• Detection Notes: Detection Method: GE ImageQuant LAS 4000 mini plus ECL

Comments

• Comments: The cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

reviewed by: Verified Customer WB Mouse 12/14/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

Blocking

• Blocking Details: Blocking Buffer: 5% BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab Dilution Ratio: 1:10000

Details

• Detection Notes: Detection Method: HRP System, detect ECL signal by photographic film

reviewed by: Verified Customer WB Human 12/08/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat milk

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse IgG HRP

Details

• Detection Notes: Detection Method: ECL

reviewed by: Verified Customer WB Rat 12/06/2011

Summary

• Application: Western Blot
• Sample Tested: Rat
• Species: Rat

Blocking

• Blocking Details: Blocking Buffer: 5% Milk

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 1 hour

Secondary Antibody

• Secondary Description: Secondary Ab: Mouse

Details

• Detection Notes: Detection Method: ECL

reviewed by: Verified Customer WB Human 12/05/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human
• Comments: Good!

Blocking

• Blocking Details: Blocking Buffer: 3% BSA, Blocking Time: 2 hours

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 16 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: Mouse Millipore, Secondary Ab Dilution Ratio: 1:10000

Details

• Detection Notes: Detection Method: ECL Detection

Comments

• Comments: Good!

reviewed by: Verified Customer WB Mouse 11/30/2011

Summary

• Application: Western Blot
• Sample Tested: mouse brain
• Species: Mouse

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat dry milk in PBS

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: Anti-Mouse IgG HRP, Secondary Ab Dilution Ratio: 1:10000

Details

• Detection Notes: Detection Method: ECL

reviewed by: Verified Customer WB Human 11/21/2011

Summary

• Application: Western Blot
• Sample Tested: Human Cell lines
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% TTBS

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse

Details

• Detection Notes: Detection Method: ECL

reviewed by: Verified Customer WB Other 11/09/2011

Summary

• Application: Western Blot
• Sample Tested: Chicken cell lines
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: Anti-mouse HRP

Details

• Detection Notes: Detection Method: ECL

reviewed by: Wei-I Shiao WB Mouse 09/02/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse Cells
• Species: Mouse

Blocking

• Blocking Details: Blocking Buffer: HyBlock blocking buffer from HyCell Biotec, Blocking Time: 1 minute

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 10 minutes

Secondary Antibody

• Secondary Description: Secondary Ab: Mouse IgG

Details

• Detection Notes: Detection Method: Viaglo HRP Chemilum Substrate Kit, Negative Control: Beta Actin

reviewed by: Courtney Kurtz WB Other 08/11/2011

Summary

• Application: Western Blot
• Sample Tested: Ground squirrel small intestinal lysate, Sample Amount: 50 ug
• Species: Other
• Comments: Antibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!

Blocking

• Blocking Details: Blocking Buffer: 4% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000, Incubation Dilution Buffer: 4% milk, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

• Secondary Description: Secondary Ab: Goat anti-mouse poly-HRP, Secondary Ab Dilution Ratio: 1:1500

Details

• Detection Notes: Detection Method: Opti-4CN, Exposure Time: < 10 s, Specific Bands: ~42 kDa

Comments

• Comments: Antibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!

reviewed by: WB Mouse 08/30/2010

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate, adult neural stem cell whole cell lysate
• Species: Mouse
• Comments: Very strong detection with 1:5000 dilution

Comments

• Comments: Very strong detection with 1:5000 dilution

FAQs for beta-Actin Antibody (NB600-501). (Showing 1 - 10 of 12 FAQs).

1. Why is beta actin used as a loading control in blotting ?
   • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
2. Are the beta-Actin antibodies validated in Simple Western?
   • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
3. Do Beta-Actin antibodies come in lyophilized format?
   • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
4. What is the theoretical molecular weight for Beta-Actin antibodies?
   • The TMW for beta Actin antibodies is approximately 42 kDa.
5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
   • For homogenized tissue, beta-actin and GAPDH are both fine.
6. We have these Abs: NB120-19481 PPAR gamma Antibody and NB600-501 Beta Actin Antibody (AC-15). The samples that we are analyzing are bovine ones. Please, confirm me if those proteins are valid like positive controls in WB for those antibodies: 1. Beta Actin Recombinant Protein (H00000060-P01). 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). Or perhaps could be better those lysates: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02 3. PPAR gamma Lysate - NBL1-14634 4. PPAR gamma Lysate - NBL1-14633 5. PPAR gamma Lysate - NBL1-14635.
   • I recommend the below controls as we have validated these as controls. NB120-19481: 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). NB600-501: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02
7. I would like to try the Beta Actin Antibody (AC-15) you have in stock to see if it reacts with fish samples in a Western Blot. Also, I would like to know if you have more information about its reactivity with fish?
   • We have tested that this antibody NB600-501 is reactive with fish. It has been tested with use in black carp. The homology with black carp is 99.0%.
8. Could anti-beta actin antibody recognize the actin derived from mosquito cells (C6/36)?
   • Unfortunately we have not used our antibody with catalogue number NB600-501 to detect beta actin that is derived from mosquito cells. You may however wish to align the peptide immunogen sequence against the full length mosquito beta actin sequence to check the homology. We usually suggest that a homology of greater than 80% is indicative of likely binding. The immunogen sequence for NB600-501 is Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (DDDIAALVIDNGSGK).
9. May we ask if NB600-501 has been tested with samples from zebrafish?
   • Beta Actin is a highly conserved protein and yes, our Beta Actin antibody (clone AC-15) NB600-501 is guaranteed for Zebrafish species.
10. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence (Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys) within the N-terminal region of beta Actin, conjugated to KLH.
11. Could you please tell me the concentration of beta-Actin Antibody (AC-15) (Nb600-501SS), LOT#077M4757V.
    • This antibody is unpurified (provided as ascites fluid) and hence the antibody concentration is non-quantifiable.
12. I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828? 
    • I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.  
Show All 12 FAQs.

Bioinformatics

• Gene Symbol: ACTB