beta-Actin Antibody (AC-15)

Images

 
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount ...read more
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Beta-actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 ...read more
Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (orange) and Isotype control antibody (blue).
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.
Immunohistochemistry: beta-Actin Antibody (AC-15) [NB600-501] - Breast, Epithelium 40X.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - HS-68 cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)Species Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP
Clone
AC-15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

beta-Actin Antibody (AC-15) Summary

Immunogen
This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Epitope
N-terminus of the beta isoform of actin.
Specificity
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixing and paraffin-embedding.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:10000
  • Simple Western 1:25
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size: Wes/Sally Sue/Peggy Sue.

Use in immunoprecipitation reported in scientific literature (PMID: 23647384). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 26414348).
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
beta-Actin Knockout 293T Cell Lysate
Reviewed Applications
Read 25 Reviews rated 4.8
using
NB600-501 in the following applications:

Publications
Read Publications using
NB600-501 in the following applications:

  • 1 publication
  • 2 publications
  • 3 publications
  • IHC
    3 publications
  • IP
    2 publications
  • KD
    1 publication
  • WB
    255 publications

Reactivity Notes

The antibody cross reacts with beta-actin expressing cells in carp, leech tissues (Hirudo medicinalis), ground squirrel. Does not cross react with adult cardiac, skeletal muscle, drosophila or amoeba beta actin. Mammal reactivity and Hamster reactivity reported in scientific literature (PMID: 25130694 and 24478435 respectively). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for beta-Actin Antibody (AC-15)

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • anti-beta actin
  • B-Actin
  • beta actin (ac-15)
  • Beta Actin
  • beta cytoskeletal actin
  • beta-actin ac 15
  • beta-actin ac15
  • beta-actin ac-15
  • betaActin
  • beta-Actin
  • BRWS1
  • I(2)-Actin
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References
1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP
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Publications for beta-Actin Antibody (NB600-501)(363)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Fish, Hamster, Mammal, Porcine, Rabbit, Squirrel, Zebrafish.

We have publications tested in 7 applications: ChIP, ELISA, ICC/IF, IHC, IP, KD, WB.


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ChIP
(1)
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(2)
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(3)
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(1)
WB
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Human
(135)
Mouse
(84)
Rat
(28)
Bovine
(1)
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(2)
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(3)
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(1)
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(2)
Mammal
(1)
Porcine
(3)
Rabbit
(5)
Squirrel
(1)
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Showing Publications 1 - 10 of 363. Show All 363 Publications.
Publications using NB600-501 Applications Species
Zhou TE, Zhu T, Rivera JC et al. The Inability of the Choroid to Revascularize in Oxygen-Induced Retinopathy Results from Increased P53/mir-Let-7b Activity Am. J. Pathol. Aug 17 2019 [PMID: 31430465]
Li MY, Peng WH, Wu CH et al. PTPN3 suppresses lung cancer cell invasiveness by counteracting Src-mediated DAAM1 activation and actin polymerization Oncogene Aug 12 2019 [PMID: 31406243] (KD) KD
Stroik S, Kurtz K, Hendrickson EA CtIP is essential for telomere replication Nucleic Acids Res. Aug 5 2019 [PMID: 31378812] (WB, Human) WB Human
Hao C, Liu G, Tian G Autophagy inhibition of cancer stem cells promotes the efficacy of cisplatin against non-small cell lung carcinoma Ther Adv Respir Dis Aug 2 2019 [PMID: 31368411] (WB, Human) WB Human
Hayward D, Kouznetsova VL, Pierson HE et al. ANKRD9 is a metabolically-controlled regulator of IMPDH2 abundance and macro-assembly J. Biol. Chem. Jul 23 2019 [PMID: 31337707] (WB, Human) WB Human
Tsolou A, Lamprou I, Fortosi AO et al. Stemness' and 'senescence' related escape pathways are dose dependent in lung cancer cells surviving post irradiation Life Sci. Jun 12 2019 [PMID: 31201845] (WB, Human) WB Human
Galasso C. Marine Biotechnology: a sea of new resources and solutions for ocean and human health. Thesis. Jul 9 1905 (Human) Human
Wangsa D, Braun R, Stuelten CH et al. Induced Chromosomal Aneuploidy Results in Global and Consistent Deregulation of the Transcriptome of Cancer Cells Neoplasia 2019 Jul 01 [PMID: 31174021] (WB, Human) WB Human
Bai X, Ihara E, Otsuka Y et al. Mutations in KARS cause a severe neurological and neurosensory disease with optic neuropathy Hum. Mutat. May 22 2019 [PMID: 31116475] (WB, Human) WB Human
Miktosz A, tukaszuk B, Baranowski M et al. Nerve regeneration in rat peripheral nerve allografts: Evaluation of cold-inducible RNA-binding protein in nerve storage and regeneration J. Comp. Neurol. May 22 2019 [PMID: 31116410]
Show All 363 Publications.

Reviews for beta-Actin Antibody (NB600-501) (25) 4.825

Average Rating: 4.8
(Based on 25 reviews)
We have 25 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB600-501:
Filter by Applications
WB
(25)
All Applications
Filter by Species
Human
(13)
Mouse
(7)
Rat
(2)
Other
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
John Morgan
WB Human 01/31/2019
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 CELLS
SpeciesHuman
Lot106M4825V-4
 
reviewed by:
Anthony Morales
WB Rat 01/28/2019
View

Summary

ApplicationWestern Blot
Sample Tested Mouse brain
SpeciesRat
Lot014M4759
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 01/24/2019
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
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reviewed by:
Luiz Alexandre Magno
WB Mouse 07/17/2018
View

Summary

ApplicationWestern Blot
Sample Tested Mouse brain,Brain (corpus striatum)
SpeciesMouse
Lot014M4759

Comments

CommentsI have used this antibody for several years and it has worked perfectly in our western blots. It works very well at 1:10,00 dilution when loading gels with 30 ug of protein extracts. I congratulate Novus Biologicals for this achievement.
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 02/20/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
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reviewed by:
Liyong Zhang
WB Human 11/25/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 06/05/2015
View

Summary

ApplicationWestern Blot
Sample Testedhuman glioma cells
SpeciesHuman

Blocking

Blocking Details10% milk in TBST

Details

Detection NotesECL western blotting detection reagent
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 05/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedFibroblast Hs68 cell lysate
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 25146190

Details

Detection NotesSee PMID: 25146190

Comments

CommentsEffects of glucose restriction (GR) on the expression of Nampt protein in Hs68 cells. beta-Actin used as a loading control. See PMID: 25146190 for details
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 03/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedCaco-2, T84, HCT116, HT29, DU145, BPH1, Hela
SpeciesHuman

Blocking

Blocking Details1 hour at RT in 5% milk powder solution (in 1xTBST)

Primary Anitbody

Dilution RatioDilution:1/15000, 1 hour at RT

Secondary Antibody

Secondary DescriptionHRP

Details

Detection NotesECL detection
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Other 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/4000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionGoat anti-Mouse 680 RD - Licor Biosciences
Secondary Manufacturer Cat#925-68070
Secondary Concentration1/20,000

Details

Detection NotesLicor Odyssey
Western Blot beta-Actin NB600-501
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reviewed by:
Sandra Vieira
WB Human 01/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates: CHO, GC-1, Hela, Cos-7, SH-SY5Y
SpeciesHuman
Lot083M4851

Blocking

Blocking Detailsblocking solution was added for 1h (5% non-fat dry milk in 1x TBS-T)

Primary Anitbody

Dilution RatioPrimary antibody B-Actin (1:10000), diluted in 3% non-fat dry milk in 1x TBS-T, was added and incubated with membranes 1h

Secondary Antibody

Secondary DescriptionGoat anti-mouse
Secondary Concentration1:5000

Details

Detection Notesthe enhanced chemiluminescence (ECL) detection method; Homemade ECL 1 min; Exposure time: 5 min
Western Blot beta-Actin NB600-501
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reviewed by:
Liyong Zhang
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF
Western Blot beta-Actin NB600-501
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reviewed by:
Beatrice Gini
WB Human 12/02/2013
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Summary

ApplicationWestern Blot
Sample Testedhuman brain cells
SpeciesHuman
CommentsClin Cancer Res. 2013 Oct 15;19(20):5722-32.

Blocking

Blocking Details5% BSA-TBS 1X-Tween 0.1% in dH2O, 1h, RT

Primary Anitbody

Dilution Ratio1:10000, 1 h, RT, in TBS 1X-Tween 0.1% in dH2O

Secondary Antibody

Secondary Descriptionanti-mouse, 1:5000

Details

Detection NotesThermo detection reagent

Comments

CommentsClin Cancer Res. 2013 Oct 15;19(20):5722-32.
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 12/21/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% FBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Mouse 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse BALB/c macrophage cell line
SpeciesMouse
CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in PBST, Blocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Mouse IgG (HRP), Jackson

Details

Detection NotesDetection Method: GE ImageQuant LAS 4000 mini plus ECL

Comments

CommentsThe cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Mouse 12/14/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: HRP System, detect ECL signal by photographic film
Western Blot beta-Actin NB600-501
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reviewed by:
Anonymous
WB Human 12/08/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse IgG HRP

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Rat 12/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 1 hour

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Human 12/05/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 3% BSA, Blocking Time: 2 hours

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 16 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse Millipore, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL Detection

Comments

CommentsGood!
 
reviewed by:
Anonymous
WB Mouse 11/30/2011
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat dry milk in PBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Mouse IgG HRP, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell lines
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% TTBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse

Details

Detection NotesDetection Method: ECL
 
reviewed by:
Anonymous
WB Other 11/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell lines
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-mouse HRP

Details

Detection NotesDetection Method: ECL
Western Blot beta-Actin NB600-501
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reviewed by:
Wei-I Shiao
WB Mouse 09/02/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse Cells
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: HyBlock blocking buffer from HyCell Biotec, Blocking Time: 1 minute

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 10 minutes

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse IgG

Details

Detection NotesDetection Method: Viaglo HRP Chemilum Substrate Kit, Negative Control: Beta Actin
Western Blot beta-Actin NB600-501
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reviewed by:
Courtney Kurtz
WB Other 08/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedGround squirrel small intestinal lysate, Sample Amount: 50 ug
SpeciesOther
CommentsAntibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!

Blocking

Blocking DetailsBlocking Buffer: 4% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Incubation Dilution Buffer: 4% milk, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-mouse poly-HRP, Secondary Ab Dilution Ratio: 1:1500

Details

Detection NotesDetection Method: Opti-4CN, Exposure Time: < 10 s, Specific Bands: ~42 kDa

Comments

CommentsAntibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!
 
reviewed by:
WB Mouse 08/30/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate, adult neural stem cell whole cell lysate
SpeciesMouse
CommentsVery strong detection with 1:5000 dilution

Comments

CommentsVery strong detection with 1:5000 dilution

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB600-501). (Showing 1 - 10 of 11 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What is the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW for beta Actin antibodies is approximately 42 kDa.
  5. We have these Abs: NB120-19481 PPAR gamma Antibody and NB600-501 Beta Actin Antibody (AC-15). The samples that we are analyzing are bovine ones. Please, confirm me if those proteins are valid like positive controls in WB for those antibodies: 1. Beta Actin Recombinant Protein (H00000060-P01). 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). Or perhaps could be better those lysates: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02 3. PPAR gamma Lysate - NBL1-14634 4. PPAR gamma Lysate - NBL1-14633 5. PPAR gamma Lysate - NBL1-14635.
    • I recommend the below controls as we have validated these as controls. NB120-19481: 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). NB600-501: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02
  6. I would like to try the Beta Actin Antibody (AC-15) you have in stock to see if it reacts with fish samples in a Western Blot. Also, I would like to know if you have more information about its reactivity with fish?
    • We have tested that this antibody NB600-501 is reactive with fish. It has been tested with use in black carp. The homology with black carp is 99.0%.
  7. Could anti-beta actin antibody recognize the actin derived from mosquito cells (C6/36)?
    • Unfortunately we have not used our antibody with catalogue number NB600-501 to detect beta actin that is derived from mosquito cells. You may however wish to align the peptide immunogen sequence against the full length mosquito beta actin sequence to check the homology. We usually suggest that a homology of greater than 80% is indicative of likely binding. The immunogen sequence for NB600-501 is Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (DDDIAALVIDNGSGK).
  8. May we ask if NB600-501 has been tested with samples from zebrafish?
    • Beta Actin is a highly conserved protein and yes, our Beta Actin antibody (clone AC-15) NB600-501 is guaranteed for Zebrafish species.
  9. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence (Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys) within the N-terminal region of beta Actin, conjugated to KLH.
  10. Could you please tell me the concentration of beta-Actin Antibody (AC-15) (Nb600-501SS), LOT#077M4757V.
    • This antibody is unpurified (provided as ascites fluid) and hence the antibody concentration is non-quantifiable.
  11. Show All 11 FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB110-67828AF405
Alexa Fluor 488 NB110-67828AF488
Alexa Fluor 647 NB110-67828AF647
Alexa Fluor 700 NB110-67828AF700
DyLight 350 NB110-67828UV
DyLight 405 NB110-67828V
DyLight 488 NB110-67828G
DyLight 550 NB600-501R
DyLight 650 NB600-501C
DyLight 680 NB110-67828FR
DyLight 755 NB110-67828IR
HRP NB600-501H
PerCP NB110-67828PCP

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Application Focus: I see an increase in LC3, now what?
By Christina Towers, PhD. Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even small changes in signali...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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John Morgan
01/31/2019
Application: WB
Species: Human

 
Anthony Morales
01/28/2019
Application: WB
Species: Rat

Anonymous
01/24/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB