beta-Actin Antibody (AC-15)

Images

 
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Cell line lysates were separated on SDS-PAGE and probed with 1:5000 Monoclonal Anti-beta-Actin Clone: AC-15 . The antibody was developed using Goat Anti-Mouse ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin antibody on mouse fibroblasts. Image from verified customer review.
Immunohistochemistry: beta-Actin Antibody (AC-15) [NB600-501] - Staining of formalin-fixed, paraffin-embedded human heart sections with 1:1000 Monoclonal Anti-beta-Actin Clone: AC-15.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MMP-11 expression in oral cancer cell lines and the MMP-11 knockdown in SCC9 cells reduce cell migration. Western blot analysis showing the expression of MMP-11 ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HSP90 and HSP70 interact with PABPN1. Interaction of PABPN1 with HSP90. Lysates from HEK293 cells transfected with Flag-HSP90 and GFP-tagged A17-PABPN1 were ...read more
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - GFAP and IL-6 are elevated in cultures of spinal cord compared to cortical astrocytes. ((G, H) Analysis of protein homogenates from brain or spinal cord astrocyte ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - HS-68 cells.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 ...read more
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-)Species Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, ChIP, KD, KO
Clone
AC-15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta-Actin Antibody (AC-15) Summary

Immunogen
This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Epitope
N-terminus of the beta isoform of actin.
Specificity
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixing and paraffin-embedding.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
  • Simple Western 1:25
  • Western Blot 1:5000 - 1:10000
Application Notes
Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:25-1:100; matrix was 12-230 kDa.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 29 Reviews rated 4.8
using
NB600-501 in the following applications:

Publications
Read Publications using
NB600-501 in the following applications:

Reactivity Notes

The antibody cross reacts with beta-actin expressing cells in carp, leech tissues (Hirudo medicinalis), ground squirrel. Does not cross react with adult cardiac, skeletal muscle, drosophila or amoeba beta actin. Mammal reactivity and Hamster reactivity reported in scientific literature (PMID: 25130694 and 24478435 respectively). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Human reactivity reported in scientific literature (PMID:32938681) Use in Turkey reported in scientific literature (PMID:27816932).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for beta-Actin Antibody (AC-15)

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • anti-beta actin
  • B-Actin
  • beta actin (ac-15)
  • Beta Actin
  • beta cytoskeletal actin
  • beta-actin ac 15
  • beta-actin ac15
  • beta-actin ac-15
  • betaActin
  • beta-Actin
  • BRWS1
  • I(2)-Actin
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
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Publications for beta-Actin Antibody (NB600-501)(554)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Bovine, C. elegans, Canine, Chicken, Drosophila melanogaster, Fish, Hamster, Mammal, Porcine, Rabbit, Squirrel, Turkey, Zebrafish.

We have publications tested in 15 applications: B/N, Chemotaxis, Cytometric Bead Assay Standard, ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, KD, KO, SW, Simple Western, WB.


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B/N
(6)
Chemotaxis
(1)
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(1)
ELISA
(2)
FLOW
(1)
ICC/IF
(3)
IF/IHC
(6)
IHC-Fr
(1)
IHC-P
(1)
IP
(3)
KD
(2)
KO
(1)
SW
(1)
Simple Western
(1)
WB
(393)
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Human
(199)
Mouse
(131)
Rat
(43)
Bovine
(1)
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(1)
Canine
(2)
Chicken
(3)
Drosophila melanogaster
(1)
Fish
(1)
Hamster
(2)
Mammal
(1)
Porcine
(4)
Rabbit
(5)
Squirrel
(1)
Turkey
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 554. Show All 554 Publications.
Publications using NB600-501 Applications Species
Honkanen TJ, Luukkainen MEK, Koivunen JP. Role of human epidermal growth factor receptor 3 in treatment resistance of anaplastic lymphoma kinase translocated non-small cell lung cancer Cancer Biol Ther 2023-12-31 [PMID: 37722715] (WB) WB
Cottarelli A, Shahriar S, Arac A et al. Rab7a activation promotes degradation of select tight junction proteins at the blood-brain barrier after ischemic stroke bioRxiv 2023-08-31 [PMID: 37693406]
Papadaki V, Erpapazoglou Z, Kokkori M et al. IQGAP1 mediates the communication between the nucleus and the mitochondria via NDUFS4 alternative splicing NAR Cancer 2023-08-24 [PMID: 37636315]
Pai YL, Lin YJ, Peng WH et al. The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy Cell Death & Disease 2023-08-22 [PMID: 37607937]
Bow YD, Ko CC, Chang WT et al. A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage Cancer Cell International 2023-08-16 [PMID: 37587444] (WB) WB
Shih PC, Mao YW, Hu JW et al. Development of Ultrapure and Potent Tannic Acids as a Pan-coronal Antiviral Therapeutic ACS Pharmacology & Translational Science 2022-06-10 [PMID: 37582235] (WB, B/N) WB, B/N
Nishikawa Y, Matsuo Y, Watanabe R et al. Hepatocyte-specific damage in acute toxicity of sodium ferrous citrate: Presentation of a human autopsy case and experimental results in mice Toxicology reports 2023-05-30 [PMID: 37304377] (WB, Mouse) WB Mouse
Sidor K, Jeznach A, Hoser G, Skirecki T 1-Methylnicotinamide (1-MNA) inhibits the activation of the NLRP3 inflammasome in human macrophages International immunopharmacology 2023-06-06 [PMID: 37290319] (WB, Human) WB Human
Rong Z, Yang L, Chen Y et al. Sophoridine alleviates hyperalgesia and anxiety-like behavior in an inflammatory pain mouse model induced by complete freund's adjuvant Molecular pain 2023-05-19 [PMID: 37207346] (WB, Mouse) WB Mouse
Douglas C, Lomeli N, Lepe J et al. Discovery and Validation of Novel LonP1 and Proteasome Inhibitor in IDH1-R132H Malignant Astrocytoma Models bioRxiv 2023-04-21 [PMID: 37131786] (WB, B/N) WB, B/N
Show All 554 Publications.

Reviews for beta-Actin Antibody (NB600-501) (29) 4.829

Average Rating: 4.8
(Based on 29 reviews)
We have 29 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB600-501:
Filter by Applications
WB
(28)
ICC
(1)
All Applications
Filter by Species
Human
(15)
Mouse
(9)
Rat
(2)
Other
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Wei Yang Sit
WB Human 05/29/2023
View

Summary

ApplicationWestern Blot
Sample TestedAGS human gastric adenocarcinoma cell line
SpeciesHuman
Lot22190126
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 02/14/2023
View

Summary

ApplicationWestern Blot
Sample Testedmurine bone marrow derived macrophages
SpeciesMouse
Lot22190126
Immunocytochemistry beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 01/22/2022
View

Summary

ApplicationImmunocytochemistry
Sample Testedfibroblasts
SpeciesMouse
LotAC-15
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Human 05/16/2021
View

Summary

ApplicationWestern Blot
Sample TestedHEK293
SpeciesHuman
Lot90942
  5
reviewed by:
John Morgan
WB Human 01/31/2019
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 CELLS
SpeciesHuman
Lot106M4825V-4
  5
reviewed by:
Anthony Morales
WB Rat 01/28/2019
View

Summary

ApplicationWestern Blot
Sample TestedMouse brain
SpeciesRat
Lot014M4759
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Human 01/24/2019
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Luiz Alexandre Magno
WB Mouse 07/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedBrain (corpus striatum),Mouse brain
SpeciesMouse
Lot014M4759

Comments

CommentsI have used this antibody for several years and it has worked perfectly in our western blots. It works very well at 1:10,00 dilution when loading gels with 30 ug of protein extracts. I congratulate Novus Biologicals for this achievement.
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Human 02/20/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Liyong Zhang
WB Human 11/25/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
Lot105M4825V-3
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/05/2015
View

Summary

ApplicationWestern Blot
Sample Testedhuman glioma cells
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Human 05/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedFibroblast Hs68 cell lysate
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedCaco-2, T84, HCT116, HT29, DU145, BPH1, Hela
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Other 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Sandra Vieira
WB Human 01/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates: CHO, GC-1, Hela, Cos-7, SH-SY5Y
SpeciesHuman
Lot083M4851
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Liyong Zhang
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
  5
reviewed by:
Beatrice Gini
WB Human 12/02/2013
View

Summary

ApplicationWestern Blot
Sample Testedhuman brain cells
SpeciesHuman
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line
SpeciesHuman
  5
reviewed by:
Verified Customer
WB Mouse 12/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse BALB/c macrophage cell line
SpeciesMouse
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 12/14/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
Western Blot beta-Actin NB600-501
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/08/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
  4
reviewed by:
Verified Customer
WB Rat 12/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat
  5
reviewed by:
Verified Customer
WB Human 12/05/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
  5
reviewed by:
Verified Customer
WB Mouse 11/30/2011
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain
SpeciesMouse
  5
reviewed by:
Verified Customer
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell lines
SpeciesHuman
  5
reviewed by:
Verified Customer
WB Other 11/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell lines
SpeciesOther
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Wei-I Shiao
WB Mouse 09/02/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse Cells
SpeciesMouse
Western Blot beta-Actin NB600-501
Enlarge
5
reviewed by:
Courtney Kurtz
WB Other 08/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedGround squirrel small intestinal lysate, Sample Amount: 50 ug
SpeciesOther
  5
reviewed by:
Verified Customer
WB Mouse 08/30/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate, adult neural stem cell whole cell lysate
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB600-501). (Showing 1 - 10 of 12 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What is the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW for beta Actin antibodies is approximately 42 kDa.
  5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
    • For homogenized tissue, beta-actin and GAPDH are both fine.
  6. We have these Abs: NB120-19481 PPAR gamma Antibody and NB600-501 Beta Actin Antibody (AC-15). The samples that we are analyzing are bovine ones. Please, confirm me if those proteins are valid like positive controls in WB for those antibodies: 1. Beta Actin Recombinant Protein (H00000060-P01). 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). Or perhaps could be better those lysates: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02 3. PPAR gamma Lysate - NBL1-14634 4. PPAR gamma Lysate - NBL1-14633 5. PPAR gamma Lysate - NBL1-14635.
    • I recommend the below controls as we have validated these as controls. NB120-19481: 2. PPAR gamma Protein (NBP1-45290) 3. PPAR gamma Protein (H00005468-P01) 4. PPAR gamma Protein (H00005468-Q01). NB600-501: 1. Beta Actin Lysate - L015T6 2. Beta Actin Lysate - H00000060-T02
  7. I would like to try the Beta Actin Antibody (AC-15) you have in stock to see if it reacts with fish samples in a Western Blot. Also, I would like to know if you have more information about its reactivity with fish?
    • We have tested that this antibody NB600-501 is reactive with fish. It has been tested with use in black carp. The homology with black carp is 99.0%.
  8. Could anti-beta actin antibody recognize the actin derived from mosquito cells (C6/36)?
    • Unfortunately we have not used our antibody with catalogue number NB600-501 to detect beta actin that is derived from mosquito cells. You may however wish to align the peptide immunogen sequence against the full length mosquito beta actin sequence to check the homology. We usually suggest that a homology of greater than 80% is indicative of likely binding. The immunogen sequence for NB600-501 is Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (DDDIAALVIDNGSGK).
  9. May we ask if NB600-501 has been tested with samples from zebrafish?
    • Beta Actin is a highly conserved protein and yes, our Beta Actin antibody (clone AC-15) NB600-501 is guaranteed for Zebrafish species.
  10. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence (Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys) within the N-terminal region of beta Actin, conjugated to KLH.
  11. Show All 12 FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on beta-Actin.

Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

mFlour Violet Conjugated Antibodies

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05/29/2023
Application: WB
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Bioinformatics

Gene Symbol ACTB