HIF-1 alpha Antibody (241809) [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 μM CoCl2 ...read more
Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 116 ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO overnight. PVDF membrane was ...read more
HIF‑1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF‑1 alpha knockout (KO) HeLa Human Cell Line cell line using Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IP, KO
Clone
241809
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

HIF-1 alpha Antibody (241809) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1
Specificity
Detects human, mouse, and rat HIF-1 alpha.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HIF1A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunoprecipitation 1-3 ug/500 ug cell lysate
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB1536 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIF-1 alpha Antibody (241809) [Unconjugated]

  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF 1A
  • HIF1 alpha
  • HIF-1 alpha
  • HIF1A
  • HIF-1a
  • HIF-1-alpha
  • HIF1-alpha
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • MOP1HIF1-ALPHA
  • PAS domain-containing protein 8
  • PASD8alpha subunit (basic helix-loop-helix transcription factor)

Background

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIF-1 alpha Antibody (MAB1536)(25)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 4 applications: Flow, ICC, IHC Fresh, WB.


Filter By Application
Flow
(1)
ICC
(1)
IHC Fresh
(1)
WB
(22)
All Applications
Filter By Species
Human
(17)
Mouse
(6)
Rat
(1)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using MAB1536 Applications Species
S Jalili-Fir, R Prantil-Ba, A Jiang, R Potla, T Mammoto, JC Weaver, TC Ferrante, HJ Kim, JMS Cabral, O Levy, DE Ingber Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip Cell Death Dis, 2018;9(2):223. 2018 [PMID: 29445080] (WB,, Human) WB Human
K Rao, K Sethi, J Ischia, L Gibson, L Galea, L Xiao, M Yim, M Chang, N Papa, D Bolton, A Shulkes, GS Baldwin, O Patel Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent PLoS ONE, 2017;12(7):e0180028. 2017 [PMID: 28686686] (WB,, Human) WB Human
CV Park, IG Ivanova, NS Kenneth XIAP upregulates expression of HIF target genes by targeting HIF1? for Lys63-linked polyubiquitination Nucleic Acids Res., 2017;0(0):. 2017 [PMID: 28666324] (WB,, Human) WB Human
AP Fischer, SL Miles Silencing HIF-1? induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells Biochem. Biophys. Res. Commun., 2017;0(0):. 2017 [PMID: 28601635] (WB,, Human) WB Human
P Hernansanz, E Ramos, E Navarro, E Parada, N Sánchez-Ló, L Peláez-Agu, JD Cabrera-Ga, D Tello, I Buendia, A Marina, J Egea, MG López, A Bogdanova, A Martínez-R Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia Redox Biol, 2017;12(0):1040-1051. 2017 [PMID: 28511347] (WB,, Bovine) WB Bovine
M Sone, N Morone, T Nakamura, A Tanaka, K Okita, K Woltjen, M Nakagawa, JE Heuser, Y Yamada, S Yamanaka, T Yamamoto Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency Cell Metab., 2017;25(5):1103-1117.e6. 2017 [PMID: 28467928] (ICC,, Mouse) ICC Mouse
P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017;8(17):28074-28092. 2017 [PMID: 28427209] (WB,, Human) WB Human
M van Gijsel, K Baranger, P Benech, S Rivera, M Khrestchat, M de Reggi, B Gharib Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine PLoS ONE, 2017;12(4):e0175369. 2017 [PMID: 28410378] (WB,, Mouse) WB Mouse
HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson Ferrochelatase is a therapeutic target for ocular neovascularization EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28377496] (WB,, Human) WB Human
D Labrousse-, E Martínez-A, M Corral-Esc, R Bienes-Mar, J Berridy, L Serrano-Ov, E Conde, ML García-Ber, JM Giménez-Ba, AS Salinas-Sá, R Sánchez-Pr, M Yao, M Lasa, MJ Calzada VHL promotes immune response against renal cell carcinoma via NF-?B-dependent regulation of VCAM-1 J. Cell Biol, 2017;0(0):. 2017 [PMID: 28235946] (WB,, Human) WB Human
Show All 25 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HIF-1 alpha Antibody (MAB1536). (Showing 1 - 4 of 4 FAQs).

  1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
    • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
  2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
    • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, as desired by you, I am suggesting you four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products has been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449 ) (cited in at least 31 peer reviewed publications).
  3. I would like to know, does it exist path for detection HIF 1 in the venous blood before and after revascularization of the leg?
    • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
  4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
    • The theoretical molecular weight of HIF 1-alpha is ~93kDa, however, you will likely see a band between 100-120kDa due to phosphorylation.

Secondary Antibodies

 

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Bioinformatics Tool for HIF-1 alpha Antibody (MAB1536)

Discover related pathways, diseases and genes to HIF-1 alpha Antibody (MAB1536). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on HIF-1 alpha. Showing 1-10 of 30 blog posts - Show all blog posts.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric...  Read full blog post.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Controls for HIF-1 Alpha's WB, ICC-IF, IHC, IP & FLOW Analysis
Tips on positive and negative controls for HIF-1 alpha antibodies is one of the most Frequently Asked Questions on Hypoxia and HIFs. Here are top 5 suggestions from Novus Biologicals: The degradation of HIF1 alpha is the most common issue whic...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

Showing 1-10 of 30 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol HIF1A
Uniprot