Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, and ...read more
ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ERK2 Antibody [Unconjugated]
ERK2MAP kinase isoform p42
Extracellular signal-regulated kinase 2
MAP kinase 1
MAP kinase 2
mitogen-activated protein kinase 1
Mitogen-activated protein kinase 2
protein tyrosine kinase ERK2
ERK1 and ERK2 (also
known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively.
They are part of the Ras-Raf-ERK signal transduction cascade often found
downstream of growth factor receptor activation. ERK1 and ERK2 were initially
isolated and cloned as kinases activated in response to insulin and NGF. They
are expressed in most, if not all, mammalian tissues. Dual threonine and
tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1
and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share
100% amino acid sequence identity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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