beta-Actin Antibody (937215) [Unconjugated]

Images

 
Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse ...read more
beta ‑Actin was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at ...read more
Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 ...read more
The treatment of n-BP rescued the progression of QA-induced excitotoxicity in SCA3 PPs. The control and/or SCA3 PPs were treated with or without QA (1 μM) in the presence of n-BP (0, 10, 20, and 40 μg/mL) for 12 h. ...read more
Identification of menin-associated proteins (MAPs) in breast cancer cells. (A) and (B) WES of BirA-Menin fusion proteins (A) and biotin-labeled proteins (B) in total lysates of BirA-MEN1 BioID engineered T47D or MCF-7 ...read more
Identification of MEN1-modulated genes in breast cancer cells. (A) RT-qPCR of MEN1 in T47D or MCF-7 cells treated with vehicle or MEN1 shRNA lentivirus (n = 3). (B) Quantitative Western immunoassays (WES) of menin ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
937215
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

beta-Actin Antibody (937215) [Unconjugated] Summary

Immunogen
Peptide containing a sequence at the N-terminus of human beta ‑Actin
Accession # P60709
Specificity
Detects human, mouse, and rat  beta ‑Actin in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 0.1-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.01 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.7
using
MAB8929 in the following applications:

Publications
Read Publications using
MAB8929 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Actin Antibody (937215) [Unconjugated]

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • B-Actin
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • I(2)-Actin
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Actin Antibody (MAB8929)(104)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mesocricetus auratus (Hamster), N/A, Transgenic Mouse.

We have publications tested in 4 applications: ICC, Immunoprecipitation, Simple Western, Western Blot.


Filter By Application
ICC
(2)
Immunoprecipitation
(1)
Simple Western
(8)
Western Blot
(49)
All Applications
Filter By Species
Human
(33)
Mouse
(16)
Rat
(5)
Mesocricetus auratus (Hamster)
(1)
N/A
(1)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 104. Show All 104 Publications.
Publications using MAB8929 Applications Species
Ge, D;Chen, Q;Xie, X;Li, Q;Yang, Y; Unveiling the potent effect of vitamin D: harnessing Nrf2/HO-1 signaling pathways as molecular targets to alleviate urban particulate matter-induced asthma inflammation BMC pulmonary medicine 2024-01-25 [PMID: 38273268] (Western Blot, Mouse) Western Blot Mouse
Vogt, M;Dienstbier, N;Schliehe-Diecks, J;Scharov, K;Tu, JW;Gebing, P;Hogenkamp, J;Bilen, BS;Furlan, S;Picard, D;Remke, M;Yasin, L;Bickel, D;Kalia, M;Iacoangeli, A;Lenz, T;Stühler, K;Pandyra, AA;Hauer, J;Fischer, U;Wagener, R;Borkhardt, A;Bhatia, S; Co-targeting HSP90 alpha and CDK7 overcomes resistance against HSP90 inhibitors in BCR-ABL1+ leukemia cells Cell death & disease 2023-12-06 [PMID: 38057328] (Western Blot, N/A) Western Blot N/A
Bychkov, ML;Isaev, AB;Andreev-Andrievskiy, AA;Petrov, K;Paramonov, AS;Kirpichnikov, MP;Lyukmanova, EN; A?1-42 Accumulation Accompanies Changed Expression of Ly6/uPAR Proteins, Dysregulation of the Cholinergic System, and Degeneration of Astrocytes in the Cerebellum of Mouse Model of Early Alzheimer Disease International journal of molecular sciences 2023-10-03 [PMID: 37834299] (Western Blot, Transgenic Mouse) Western Blot Transgenic Mouse
Zangiabadi, S;Chamoun, KP;Nguyen, K;Tang, Y;Sweeney, G;Abdul-Sater, AA; Copper infused fabric attenuates inflammation in macrophages PloS one 2023-09-15 [PMID: 37713400] (Western Blot, Human) Western Blot Human
Victor Bessonneau-Gaborit, Jonathan Cruard, Catherine Guerin-Charbonnel, Jennifer Derrien, Jean-Baptiste Alberge, Elise Douillard et al. Exploring the impact of dexamethasone on gene regulation in myeloma cells Life Science Alliance 2023-09-01 [PMID: 37524526]
Momčilović, S;Bogdanović, A;Milošević, MS;Mojsilović, S;Marković, DC;Kočović, DM;Vignjević Petrinović, S; Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress International journal of molecular sciences 2023-07-12 [PMID: 37511129] (Western Blot, Mouse) Western Blot Mouse
Silas Wurnig, Melina Vogt, Julian Hogenkamp, Niklas Dienstbier, Arndt Borkhardt, Sanil Bhatia et al. Development of the first geldanamycin-based HSP90 degraders Frontiers in Chemistry 2023-06-28 [PMID: 37448856]
Lin, X;Wang, M;He, Z;Hao, G; Gut microbiota mediated the therapeutic efficiency of Simiao decoction in the treatment of gout arthritis mice BMC complementary medicine and therapies 2023-06-21 [PMID: 37344836] (Western Blot, Mouse) Western Blot Mouse
Marlena Brzozowa-Zasada, Adam Piecuch, Karolina Bajdak-Rusinek, Kamil Janelt, Marek Michalski, Olesya Klymenko et al. Immunohistochemical Expression of Glutathione Peroxidase 1 (Gpx-1) as an Independent Prognostic Factor in Colon Adenocarcinoma Patients Pharmaceuticals (Basel) 2023-05-12 [PMID: 37242524]
Kumarasamy, V;Frangou, C;Wang, J;Wan, Y;Dynka, A;Rosenheck, H;Dey, P;Abel, EV;Knudsen, ES;Witkiewicz, AK; Pharmacologically targeting KRAS G12D in PDAC models: tumor cell intrinsic and extrinsic impact bioRxiv : the preprint server for biology 2023-04-20 [PMID: 37162905] (Western Blot, Human) Western Blot Human
Show All 104 Publications.

Reviews for beta-Actin Antibody (MAB8929) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using MAB8929:
Filter by Applications
Simple Western
(2)
WB
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Simple Western beta-Actin MAB8929
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5
reviewed by:
Verified Customer
Simple Western Human 08/27/2020
View

Summary

ApplicationSimple Western
Sample TestedHCT-116 human colorectal carcinoma cell line
SpeciesHuman
LotCJQV0318021
Simple Western beta-Actin MAB8929
Enlarge
4
reviewed by:
Dahao Ling
Simple Western Human 11/07/2019
View

Summary

ApplicationSimple Western
Sample TestedHuman liver
SpeciesHuman
LotCJQV0318071

Comments

CommentsOn Jess from Protein Simple
Western Blot beta-Actin MAB8929
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/15/2016
View

Summary

ApplicationWestern Blot
Sample TestedHEK293T and NIH3T3 whole cell lysate
SpeciesHuman
LotCJQV0115101

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-Actin Antibody (MAB8929). (Showing 1 - 6 of 6 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What is the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW for beta Actin antibodies is approximately 42 kDa.
  5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
    • For homogenized tissue, beta-actin and GAPDH are both fine.
  6. Does this Beta-Actin antibody come in lyophilized form?
    • Yes, we carry this beta-actin antibody in lyophilized format.

Secondary Antibodies

 

Isotype Controls

Additional beta-Actin Products

Blogs on beta-Actin.

Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
08/27/2020
Application: Simple Western
Species: Human

Dahao Ling
11/07/2019
Application: Simple Western
Species: Human

Verified Customer
03/15/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB
Uniprot