Survivin Antibody - BSA Free

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: Survivin Antibody [NB500-201] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD ...read more
Biological Strategies: Knockdown Validated: Survivin Antibody [NB500-201] - Western blot analysis using Survivin and flotillin antibodies was performed on lysates of DMSO- and PTX-treated MDAMB231 cells ...read more
Immunohistochemistry: Survivin Antibody [NB500-201] - Protein expression pattern of Survivin in HCC tissues and non-neoplastic liver parenchyma. IAP members immunoreactivity was estimated by tissue microarray in a ...read more
Genetic Strategies: Knockdown Validated: Survivin Antibody [NB500-201] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the ...read more
Immunocytochemistry/ Immunofluorescence: Survivin Antibody [NB500-201] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. ...read more
Western Blot: Survivin Antibody [NB500-201] - Analysis of 30ug of HeLa whole cell lysate [NB800-PC1] using rabbit polyclonal [NB500-201] at 1ug/ml. Detection was performed using ECL method with 1 minute exposure. Band ...read more
Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Immunohistochemical staining of Survivin in human rectal cancer using [NB500-201] and DAB with hematoxylin counterstain.
Flow Cytometry: Survivin Antibody [NB500-201] - An intracellular stain was performed on HeLa cells with [NB500-201] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more
Western Blot: Survivin Antibody [NB500-201] - Western blot analysis using Survivin Antibody [NB500-201]. Effects of siRNA transfection on the expression of Bcl-xL and survivin. Bcl-xL and survivin protein content ...read more
Western Blot: Survivin Antibody [NB500-201] - Analysis of Survivin in human hepatocytes from cancer patient (left) and HeLa cell lysate (right) using [NB500-201]. Image from verified customer review. Note: bands ...read more
Western Blot: Survivin Antibody [NB500-201] - Western blot analysis using [NB500-201]. Survivin protein is degraded by ubiquitin proteasome in serum-free and serum-containing media. Western blots and protein ...read more
Immunocytochemistry/ Immunofluorescence: Survivin Antibody [NB500-201] - Analysis of HeLa cells using Survivin Antibody ([NB500-201], 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Analysis of Survivin in ovarian cancer tissue using [NB500-201]. Image from verified customer review.
Immunohistochemistry: Survivin Antibody [NB500-201] - HRP conjugated Survivin expression in BIRC5 transfected 293T cells using Survivin Antibody [NB500-201]. Image from verified customer review.
Immunohistochemistry: Survivin Antibody [NB500-201] - Immunohistochemical analysis using [NB500-201]. The top photo is a control stain and the bottom photo is anti-survivin staining of melanoma. Photo courtesy of Dr. ...read more
Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Survivin shows lysates of human neuroblastoma cell line. Polyvinylidene fluoride (PVDF) membrane was probed with 1:200 dilution of 0.5 ug/mL of rabbit ...read more
Simple Western: Survivin Antibody [NB500-201] - Simple Western analysis using [NB500-201]. Lane view shows a specific band for Survivin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing ...read more
Radiation-resistant Caveolin-1 (CAV1)-silenced fibroblasts differentially express and secrete the apoptosis inhibiting protein TP53-regulated inhibitor of apoptosis 1 (TRIAP1).
Expression of survivin protein in cell lines after transfection with siRNA.Survivin protein expression in CHS cell lines was evaluated by western blotting at 48 h after transfection with scrambled and survivin siRNA.
Western blotting analysis in MDA-MB-231 cells and SUM 149 cells.The cells were treated for 24 h with C. juttae essential oil (EO) (46 and 64 μg/ml, respectively). The data shown are the results of a representative ...read more
(A) Levels of suppression of Survivin and expression of p53 for 2 h, 4 h and 8 h for MBA-MD 231 cells. (B) Light microscopy images of the three cell types using nanoconstruct with the AS1411 aptamer. Cell ...read more
Detection of BCL2, Bcl-xL, XIAP and survivin protein content by western blotting 48 h after transfection with a total of 40 nM siRNA in EJ28 bladder cancer cells. Beta-actin was used for loading control.
Effects of siRNA transfection on the expression of Bcl-xL and survivin. (a) Relative mRNA expression levels of Bcl-xL and survivin in EJ28 and J82 bladder cancer cells, 48 h after transfection. Expression values are ...read more
Aurora B phosphorylates EB2.(c,d) HeLa cells were transfected with siRNAs as indicated; after 24 h, the cells were treated with nocodazole for 4 h. Mitotic cells were harvested by mitotic shake-off. Image collected and ...read more
Overexpression of Survivin predominantly protects anoikis. B). Caspase-3 activation in EGFP- and EGFP-Survivin-expressing cells after serum starvation under attached or detached culture conditions. The experimental ...read more
Survivin regulates autophagic flux. (A) U2OS cells stably expressing survivinWTGFP or GFP alone were treated with RAP (200 nM) and CQ (120 uM) for 2 h, then lysed and immunoblotted with anti-LC3, anti-survivin and ...read more
Protein expression pattern of Survivin, XIAP and XAF1 in HCC tissues and non-neoplastic liver parenchyma. IAP members immunoreactivity was estimated by tissue microarray in a subset of HCC patients (n = 40). A-D, ...read more
Survivin and BIM expression in response to lapatinib. Western analysis of Survivin and BIM protein levels after 24 hour exposure to 0.1% DMSO (-) or 2 uM lapatinib (+) in lapatinib-resistant cells (A), cells ...read more
Gene-silencing of Bcl-2, Survivin and XIAP. Western blot showed efficient silencing in transfected MiaPaCa-2 (A) and AsPC-1 cells (B). 50,000 cells/well were single-transfected with carrier solution (lane 1) and siRNA ...read more
NPC-derived EBNA1 is compromised for interaction with Survivin. F). In situ Proximity Ligation Assay (in situ PLA) is shown for interphase cells from either MUTU-I or C666-1 using mouse anti-EBNA1 and rabbit ...read more
Survivin is essential for the salinomycin-induced apoptosis in UM cells. A) UM cells were treated with salinomycin for 24 h and the protein levels of apoptosis-related proteins were detected by Western blot. Image ...read more
Pro-apoptotic activity of SAB-13 and SAB-14. (A-C,E,F), FACS analysis of the cells after 48 h treatment. Analysis of apoptosis induction in 22Rv1 (A) and PC-3 cells (B) using Annexin-V-FITC/propidium iodide (PI) double ...read more
Short‐term nicotine exposure does not alter Lypd6, Lynx1, or Ly6H levels in the brain. Lypd6, Lynx1, Ly6H, and beta 2 nAChR subunit protein levels were analyzed in (a) frontal cortex (FC) and hippocampal (HIP) tissue ...read more
UroA/UAS03 enhance tight junction proteins in AhR-dependent manner. a HT29 cells were treated with vehicle (DMSO-0.01%)/UroA/UAS03 (50 μM) for 24 h. mRNA levels of Cytochrome P450 1A1 (Cyp1A1) was measured by RT ...read more
Characterization of MSCmix and NCSCmix isolated from the bone marrow of adult Wnt1-CRE/R26R-LacZ mice. After recombination, NCSCs from Wnt1-CRE/R26R-LacZ mice express LacZ gene. MSCs did not undergo Cre/Lox ...read more
Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A and B—Examples of low AIP expression; C and D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody ...read more
Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression.(A & B) Real Time RT-PCR analysis showing relative quantitation of p21 transcript level in (A) HCT116p53null cells with WT and N ...read more
Pre-treatment with 0.5 mM sodium arsenite (SA) enhances permissivity in a cell-type-specific manner across reovirus strains. (A) CV-1, HeLa, L929, or HPDE cells were left untreated (no SA) or were treated with 0.5 mM SA ...read more
Inhibition of Rac1 by N17Rac1 mutant or Rac1 siRNA diminishes IR-induced G2/M checkpoint activation. (A) MCF-7 cells were infected with Ad.N17Rac1 or Ad.Control for 24 hours and exposed to 15-Gy IR. Left panel: the ...read more
Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels ...read more
Vps13 co-fractionates with Rab7 and Rab5.(A) Western blot analysis of control fly head samples fractionated into a cytosolic and membrane fraction from postnuclear supernatant (PNS). EGFR was used as a membrane marker ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Fe, Gp, HaSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, ChIP, Dual ISH-IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Survivin Antibody - BSA Free Summary

Immunogen
This Survivin Antibody was developed against full length recombinant human Survivin [UniProt# O15392]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BIRC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Dual RNAscope ISH-IHC
  • ELISA reported in scientific literature (PMID 24102797)
  • Flow Cytometry reported in scientific literature (PMID 17875988; 33737139)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 12671708)
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Simple Western 1:25
  • Western Blot 1:1000
Application Notes
In WB, a band at approx. 16.5 kDa can be seen. For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Survivin Overexpression Lysate
Reviewed Applications
Read 22 Reviews rated 4.5
using
NB500-201 in the following applications:

Publications
Read Publications using
NB500-201 in the following applications:

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 23405201). Guinea Pig reactivity reported in scientific literature (PMID: 21364656).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Survivin Antibody - BSA Free

  • API4
  • apoptosis inhibitor 4
  • Apoptosis inhibitor survivin
  • baculoviral IAP repeat-containing 5
  • Baculoviral IAP Repeat-Containing Protein 5
  • BIRC5
  • EPR-1
  • IAP4
  • survivin variant 3 alpha
  • Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, ChIP, Dual ISH-IHC, KD

Publications for Survivin Antibody (NB500-201)(365)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Guinea Pig, Hamster.

We have publications tested in 13 applications: B/N, ELISA, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB.


Filter By Application
B/N
(2)
ELISA
(2)
FLOW
(5)
ICC/IF
(33)
IF/IHC
(45)
IHC
(2)
IHC-Fr
(2)
IHC-P
(39)
IP
(4)
KD
(3)
PLA
(3)
Simple Western
(1)
WB
(120)
All Applications
Filter By Species
Human
(178)
Mouse
(26)
Rat
(9)
Canine
(13)
Guinea Pig
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 365. Show All 365 Publications.
Publications using NB500-201 Applications Species
Krajnik A, Nimmer E, Brazzo JA et al. Survivin regulates intracellular stiffness and extracellular matrix production in vascular smooth muscle cells APL bioengineering 2023-12-01 [PMID: 37868708] (WB, Human) WB Human
Breder-Bonk C, Docter D, Barz M et al. The Apoptosis Inhibitor Protein Survivin Is a Critical Cytoprotective Resistor against Silica-Based Nanotoxicity Nanomaterials (Basel, Switzerland) 2023-09-12 [PMID: 37764575] (IHC, Mouse, Human) IHC Mouse, Human
Stahl P, Kollenda S, Sager J et al. Tuning Nanobodies' Bioactivity: Coupling to Ultrasmall Gold Nanoparticles Allows the Intracellular Interference with Survivin Small (Weinheim an der Bergstrasse, Germany) 2023-04-10 [PMID: 37035950] (ICC/IF, PLA, WB, Human) ICC/IF, PLA, WB Human
Dyshlovoy S, Shubina L, Makarieva T et al. New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells Marine Drugs 2022-11-25 [PMID: 36547885] (WB, Human) WB Human
Dyshlovoy SA, Busenbender T, Hauschild J et al. Cytotoxic N-Methylpretrichodermamide B Reveals Anticancer Activity and Inhibits P-Glycoprotein in Drug-Resistant Prostate Cancer Cells Marine Drugs 2022-09-23 [PMID: 36286421] (B/N) B/N
Fuller RN, Kabagwira J, Vallejos PA et al. Survivin Splice Variant 2? Enhances Pancreatic Ductal Adenocarcinoma Resistance to Gemcitabine OncoTargets and Therapy 2022-10-10 [PMID: 36238134] (WB) WB
Vay C, Babaei S, Safi SA et al. Clinicopathological and Prognostic Value of Survivin Expression in Surgically Resected Pancreatic Ductal Adenocarcinoma Cancers 2022-07-18 [PMID: 35884555] (IHC-P, Human) IHC-P Human
da Costa Lopes J, FalcAo LFM, Martins Filho AJ et al. Factors Involved in the Apoptotic Cell Death Mechanism in Yellow Fever Hepatitis Viruses 2022-06-01 [PMID: 35746675] (IF/IHC, Human) IF/IHC Human
Krieg S, Roderburg C, Fung S et al. Nuclear survivin is a prognosticator in gastroenteropancreatic neuroendocrine neoplasms: a meta-analysis Journal of Cancer Research and Clinical Oncology 2022-09-01 [PMID: 35428913] (IHC) IHC
Mackay RP, Weinberger PM, Copland JA et al. YM155 induces DNA damage and cell death in anaplastic thyroid cancer cells by inhibiting DNA topoisomerase IIalpha at the ATP binding site Molecular cancer therapeutics [PMID: 35405742] (Simple Western, Human) Simple Western Human
Show All 365 Publications.

Reviews for Survivin Antibody (NB500-201) (22) 4.522

Average Rating: 4.5
(Based on 22 reviews)
We have 22 reviews tested in 5 species: Human, Mouse, Rat, Human and Mouse, Other.

Reviews using NB500-201:
Filter by Applications
WB
(16)
IHC-P
(4)
ICC
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(12)
Mouse
(4)
Rat
(1)
Human and Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 05/17/2019
View

Summary

ApplicationWestern Blot
Sample TestedCardiomyocytes from newborn rats
SpeciesMouse
LotAE-4
  4
reviewed by:
Anna Herman-Antosiewicz
WB Human and Mouse 05/04/2017
View

Summary

ApplicationWestern Blot
Sample TestedHDFa Whole Cell Lysate (Human Dermal Fibroblasts,MEF whole cell lysate,PC3 Whole Cell Lysate (Human prostate cancer cell)
SpeciesHuman and Mouse
LotAG1

Comments

CommentsWe obtained a strong signal for both human (PC3, HDFa) and mouse (MEF) samples. However, in mouse samples a strong non specific band at 28 kDa was also observed.
Western Blot Survivin NB500-201
Enlarge
4
reviewed by:
Verified Customer
WB Human 01/11/2017
View

Summary

ApplicationWestern Blot
Sample TestedA549 whole cell lysate,H1299 whole cell lysate
SpeciesHuman
LotIVA061607A
  3
reviewed by:
Verified Customer
IHC-P Mouse 05/06/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse cervix
SpeciesMouse
  5
reviewed by:
Yoshihiko Kobayashi
IHC-P Rat 02/27/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedlung
SpeciesRat
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Verified Customer
WB Human 04/28/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
  4
reviewed by:
Verified Customer
WB Human 12/29/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23504997
SpeciesHuman
  5
reviewed by:
Verified Customer
IHC-P Human 12/29/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23161837
SpeciesHuman
  4
reviewed by:
Verified Customer
WB Human 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23449974
SpeciesHuman
Immunohistochemistry-Paraffin Survivin NB500-201
Enlarge
5
reviewed by:
Verified Customer
IHC-P 11/07/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Immunocytochemistry Survivin NB500-201
Enlarge
5
reviewed by:
Bryan Tinsley
ICC Human 06/16/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
  5
reviewed by:
Verified Customer
ELISA Human 05/20/2014
View

Summary

ApplicationELISA
SpeciesHuman
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/27/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotU-3
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Verified Customer
WB Human 07/19/2013
View

Summary

ApplicationWestern Blot
Sample TestedDEndritic cell lysate
SpeciesHuman
  5
reviewed by:
Masaaki Tatsuka
WB Human 03/28/2013
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotN/A
Western Blot Survivin NB500-201
Enlarge
4
reviewed by:
Hua Li
WB 08/23/2012
View

Summary

ApplicationWestern Blot
LotI can't find the original vial now
Western Blot Survivin NB500-201
Enlarge
4
reviewed by:
Verified Customer
WB 08/21/2012
View

Summary

ApplicationWestern Blot
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Astrid Spannhoff
WB Human 11/19/2010
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate, Sample Amount: 5-15 ul
SpeciesHuman
  4
reviewed by:
Verified Customer
WB Mouse 06/07/2010
View

Summary

ApplicationWestern Blot
Sample TestedCancer Cells
SpeciesMouse
  4
reviewed by:
Hope Lancero
WB Human 05/04/2010
View

Summary

ApplicationWestern Blot
Sample TestedHuman smooth muscle cells, Sample Amount: 30ug
SpeciesHuman
  3
reviewed by:
Verified Customer
WB Mouse 08/31/2009
View

Summary

ApplicationWestern Blot
Sample Testedmouse cell, Sample Amount: 50ug
SpeciesMouse
  5
reviewed by:
Verified Customer
WB Other 07/02/2009
View

Summary

ApplicationWestern Blot
Sample TestedOswald 1771 Abrams D17 Cancer Cell Lines, Sample Amount: 7-15 ug
SpeciesOther
LotR2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Survivin Antibody (NB500-201). (Showing 1 - 4 of 4 FAQs).

  1. Can I use this antibody with species other than those listed?
    • The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.
  2. We would like to ensure 3 isoforms of Survivin can all be detected before placing the order. The sample is total protein extracted from Mouse heart. May we ask if it is possible? In addition, as we know, there are 7 isoforms of Human Survivin. May we also ask the reason why there is only one band observed by HeLa (as shown on the product page)?
    • This antibody was made to the full length canonical sequence for Survivin but it has not been tested for reactivity with all isoforms of Survivin. It is possible being a polyclonal but we cannot guarantee it will react with a particular isoform.
  3. I would appreciate if you are able to confirm how many of the splice variants this antibody will detect (survivin wild type, survivin isoform 2 and also survivin 2-alpha). Has this been tested? If you have any data to show this, it would be beneficial.
    • NB500-201 was raised against the full-length survivin protein and is a polyclonal antibody, therefore we do not know where or how many sites the antibody detects. We have not tested for reactivity against specific splice variants, but any customer feedback regarding this would be greatly appreciated.
  4. I hope you can help with a quick query: apologies if I have missed this information on the technical data sheet, but where on the protein has the epitope for antibody been raised?
    • Our Survivin antibody NB500-201 was raised against the full length human survivin protein (UniProt# O15392). We unfortunately do not epitope map our antibodies, so the exact epitope recognized by this antibody has not been determined.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Survivin. Showing 1-10 of 16 blog posts - Show all blog posts.


  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Survivin - an inhibitor of apoptosis that drives tumorigenesis and metastasis
Apoptosis is the tightly regulated process of programmed cell death. It plays an important role in normal physiologic development but has also been implicated in a number of diseases. Apoptosis is constantly downregulated by a family of anti-apop...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

Survivin: As long as I know how to live I know I will
Survivin is an anti-apoptotic protein from a large family with related members such as X-linked IAP, cIAP1 and cIAP2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates fundamental physiological events including the cell cycle, feta...  Read full blog post.

Survivin: Infographic
Survivin is involved in promoting cell proliferation and is an inhibitor of apoptosis. Survivin has a critical role in cancer proliferation and neural development. It may have an impact on neural cell proliferative responses following brain injury.L...  Read full blog post.

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol BIRC5
Entrez
Uniprot