Survivin Antibody

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: Survivin Antibody [NB500-201] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD ...read more
Western Blot: Survivin Antibody [NB500-201] - Analysis of 30ug of HeLa whole cell lysate [NB800-PC1] using rabbit polyclonal [NB500-201] at 1ug/ml. Detection was performed using ECL method with 1 minute exposure. Band ...read more
Immunocytochemistry/ Immunofluorescence: Survivin Antibody [NB500-201] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. ...read more
Genetic Strategies: Knockdown Validated: Survivin Antibody [NB500-201] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the ...read more
Genetic Strategies: Knockdown Validated: Survivin Antibody [NB500-201] - Aurora B phosphorylates EB2. (c,d) HeLa cells were transfected with siRNAs as indicated; after 24 h, the cells were treated with nocodazole ...read more
Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Immunohistochemical staining of Survivin in human rectal cancer using [NB500-201] and DAB with hematoxylin counterstain.
Flow Cytometry: Survivin Antibody [NB500-201] - An intracellular stain was performed on HeLa cells with [NB500-201] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more
Western Blot: Survivin Antibody [NB500-201] - Western blot analysis using Survivin Antibody [NB500-201]. Effects of siRNA transfection on the expression of Bcl-xL and survivin. Bcl-xL and survivin protein content ...read more
Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Survivin shows lysates of human neuroblastoma cell line. Polyvinylidene fluoride (PVDF) membrane was probed with 1:200 dilution of 0.5 ug/mL of rabbit ...read more
Western Blot: Survivin Antibody [NB500-201] - Analysis of Survivin in human hepatocytes from cancer patient (left) and HeLa cell lysate (right) using [NB500-201]. Image from verified customer review. Note: bands ...read more
Western Blot: Survivin Antibody [NB500-201] - Western blot analysis using [NB500-201]. Survivin protein is degraded by ubiquitin proteasome in serum-free and serum-containing media. Western blots and protein ...read more
Immunocytochemistry/ Immunofluorescence: Survivin Antibody [NB500-201] - Analysis of HeLa cells using Survivin Antibody ([NB500-201], 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Analysis of Survivin in ovarian cancer tissue using [NB500-201]. Image from verified customer review.
Immunohistochemistry: Survivin Antibody [NB500-201] - HRP conjugated Survivin expression in BIRC5 transfected 293T cells using Survivin Antibody [NB500-201]. Image from verified customer review.
Immunohistochemistry: Survivin Antibody [NB500-201] - Immunohistochemical analysis using [NB500-201]. The top photo is a control stain and the bottom photo is anti-survivin staining of melanoma. Photo courtesy of Dr. ...read more
Simple Western: Survivin Antibody [NB500-201] - Simple Western analysis using [NB500-201]. Lane view shows a specific band for Survivin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Fe, Gp, HaSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Dual ISH-IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Survivin Antibody Summary

Immunogen
This Survivin Antibody was developed against full length recombinant human Survivin [UniProt# O15392]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BIRC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:10-1:500
  • Dual RNAscope ISH-IHC
  • Knockdown Validated
Application Notes
In WB, a band at approx. 16.5 kDa can be seen. For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin. Immunohistochemistry-Frozen, Flow Cytometry, and ELISA were reported in scientific literature (PMIDs: 12671708, 17875988, 24102797).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Survivin Overexpression Lysate
Reviewed Applications
Read 21 Reviews rated 4.4
using
NB500-201 in the following applications:

Publications
Read Publications using
NB500-201 in the following applications:

  • 2 publications
  • 2 publications
  • 2 publications
  • 29 publications
  • IHC
    42 publications
  • 2 publications
  • 37 publications
  • IP
    4 publications
  • KD
    3 publications
  • PLA
    2 publications
  • WB
    112 publications

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 23405201). Guinea Pig reactivity reported in scientific literature (PMID: 21364656).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Survivin Antibody

  • API4
  • apoptosis inhibitor 4
  • Apoptosis inhibitor survivin
  • baculoviral IAP repeat-containing 5
  • Baculoviral IAP Repeat-Containing Protein 5
  • BIRC5
  • EPR-1
  • IAP4
  • survivin variant 3 alpha
  • Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-201
Species: Hu, Mu, Rt, Ca, Fe, Gp, Ha
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Dual ISH-IHC, KD
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
NB100-56098
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56183
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-37387
Species: Hu
Applications: WB, ELISA, Flow, CyTOF-ready
NBP2-31106
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P, IP, CyTOF-ready, Flow-CS, ICC
NBP1-06579
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP2-14214
Species: Hu
Applications: IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56104
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-Fr, IHC-P, IP
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-28566
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, Func, IHC, IHC-Fr
NBP2-22471
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
NB500-213
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP
AF1280
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB500-201
Species: Hu, Mu, Rt, Ca, Fe, Gp, Ha
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Dual ISH-IHC, KD

Publications for Survivin Antibody (NB500-201)(343)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Guinea Pig, Hamster, canine.

We have publications tested in 11 applications: ELISA, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, WB.


Filter By Application
ELISA
(2)
FLOW
(2)
Flow
(2)
ICC/IF
(29)
IHC
(42)
IHC-Fr
(2)
IHC-P
(37)
IP
(4)
KD
(3)
PLA
(2)
WB
(112)
All Applications
Filter By Species
Human
(163)
Mouse
(25)
Rat
(8)
Canine
(12)
Guinea Pig
(1)
Hamster
(1)
canine
(1)
All Species
Showing Publications 1 - 10 of 343. Show All 343 Publications.
Publications using NB500-201 Applications Species
Nourreddine S, Lavoie G, Paradis J et al. NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay Cell Rep May 19 2020 [PMID: 32433969] (WB, KD, Human) WB, KD Human
Erdogan S, Turkekul K Neferine inhibits proliferation and migration of human prostate cancer stem cells through p38 MAPK/JNK activation J. Food Biochem. May 11 2020 [PMID: 32394497] (WB, Human) WB Human
Brown M, Zhang W, Yan D et al. The role of survivin in the progression of pancreatic ductal adenocarcinoma (PDAC) and a novel survivin-targeted therapeutic for PDAC PLoS ONE Jan 13 2020 [PMID: 31929540] (WB, Human) WB Human
Liang C, Zhang Z, Chen Q et al. Centromere-localized Aurora B kinase is required for the fidelity of chromosome segregation J. Cell Biol. Feb 3 2020 [PMID: 31868888]
Mirshahidi S, de Necochea-Campion R, Moretta A et al. Inhibitory Effects of Indomethacin in Human MNNG/HOS Osteosarcoma Cell Line In Vitro Cancer Invest. Nov 26 2019 [PMID: 31770037]
Zhou J, Liu S, Wang Y et al. Salinomycin effectively eliminates cancer stem-like cells and obviates hepatic metastasis in uveal melanoma Mol. Cancer Nov 13 2019 [PMID: 31718679] (WB, Human) WB Human
Abad MA, Ruppert JG, Buzuk L et al. Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex J. Cell Biol. 2019 Sep 30 [PMID: 31570499] (ICC/IF, Human) ICC/IF Human
Ong SM, Saeki K, Kok MK et al YM155 enhances the cytotoxic activity of EPE against canine osteosarcoma cells J Vet Med Sci. 2019 Jul 15 [PMID: 31308291] (IHC, Mouse) IHC Mouse
Kubo T, Tsurita G, Hirohashi Y et al. Immunohistological analysis of pancreatic carcinoma after vaccination with survivin 2B peptide: Analysis of an autopsy series Cancer Sci. Aug 1 2019 [PMID: 31206934] (IHC-P, Human) IHC-P Human
Cornago Protomartir M. Caracterizacion de los efectos de los inhibidores de las histona desacetilasas en lineas celulares de glioma Thesis. 2014 Sep 01 (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 343 Publications.

Reviews for Survivin Antibody (NB500-201) (21) 4.421

Average Rating: 4.4
(Based on 21 reviews)
We have 21 reviews tested in 5 species: Human, Mouse, Rat, Mouse and Human, Other.

Reviews using NB500-201:
Filter by Applications
WB
(15)
IHC-P
(4)
ICC
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(11)
Mouse
(4)
Rat
(1)
Mouse and Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Anonymous
WB Mouse 05/17/2019
View

Summary

ApplicationWestern Blot
Sample TestedCardiomyocytes from newborn rats
SpeciesMouse
LotAE-4
  4
reviewed by:
Anna Herman-Antosiewicz
WB Mouse and Human 05/04/2017
View

Summary

ApplicationWestern Blot
Sample TestedMEF whole cell lysate,PC3 Whole Cell Lysate (Human prostate cancer cell),HDFa Whole Cell Lysate (Human Dermal Fibroblasts
SpeciesMouse and Human
LotAG1

Comments

CommentsWe obtained a strong signal for both human (PC3, HDFa) and mouse (MEF) samples. However, in mouse samples a strong non specific band at 28 kDa was also observed.
Western Blot Survivin NB500-201
Enlarge
4
reviewed by:
Anonymous
WB Human 01/11/2017
View

Summary

ApplicationWestern Blot
Sample TestedH1299 whole cell lysate,A549 whole cell lysate
SpeciesHuman
LotIVA061607A
  3
reviewed by:
Anonymous
IHC-P Mouse 05/06/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse cervix
SpeciesMouse
  5
reviewed by:
Yoshihiko Kobayashi
IHC-P Rat 02/27/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedlung
SpeciesRat
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Anonymous
WB Human 04/28/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special Applicationshuman hepatocytes from cancer patient. Hela was used as a positive control.
FileView PDF
  4
reviewed by:
Anonymous
WB Human 12/29/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23504997
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23504997

Details

Detection NotesSee PMID 23504997

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23504997.
  5
reviewed by:
Anonymous
IHC-P Human 12/29/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23161837
SpeciesHuman
Special ApplicationsSee PMID 23161837
  4
reviewed by:
Anonymous
WB Human 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23449974
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23449974

Details

Detection NotesSee PMID 23449974

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23449974.
Immunohistochemistry-Paraffin Survivin NB500-201
Enlarge
5
reviewed by:
Anonymous
IHC-P 11/07/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Commentscostain with Gill's hematoxylin
Immunocytochemistry Survivin NB500-201
Enlarge
5
reviewed by:
Anony- mous
ICC Human 06/16/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using Survivin antibody (NB500-201, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
  5
reviewed by:
Anonymous
ELISA Human 05/20/2014
View

Summary

ApplicationELISA
SpeciesHuman
Special ApplicationsOptimal for plate coating in ELISA for detection in human serum. Special application for Immune complexes detection.
FileView PDF
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Anonymous
WB Human 03/27/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotU-3
FileView PDF
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Anonymous
WB Human 07/19/2013
View

Summary

ApplicationWestern Blot
Sample TestedDEndritic cell lysate
SpeciesHuman
CommentsNegative control: cells which do not express HA target protein. Positive control: DC expression RNA encoding for target protein. the RNA in this instance was in vitro transcribed from plasmid vector and not amplified as we did in the experimental conditions.

Blocking

Blocking Details3% BSA (Sigma) in phosphate buffered saline (PBS, Lonza) supplemented with 0.1% Tween-20 (PBS-T).

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptionanti rabbit conjugated HRP secondary antibodies
Secondary Manufacturer Cat#Santa Cruz Biotechnologies sc-2357
Secondary Concentration1:1000

Details

Detection NotesECL Plus (Pierce) and the Storm 840 Imager with the fluorescence settings. positive and negative controls are described below

Comments

CommentsNegative control: cells which do not express HA target protein. Positive control: DC expression RNA encoding for target protein. the RNA in this instance was in vitro transcribed from plasmid vector and not amplified as we did in the experimental conditions.
Western Blot Survivin NB500-201
Enlarge
4
reviewed by:
Hua Li
WB 08/23/2012
View

Summary

ApplicationWestern Blot
LotI can't find the original vial now
Pub Med ID19509229
FileView PDF
Western Blot Survivin NB500-201
Enlarge
4
reviewed by:
Anonymous
WB 08/21/2012
View

Summary

ApplicationWestern Blot
Special ApplicationsWestern Blotting and Immunofluorescence worked excellent. Have been using this product in rat for the past 5 years.
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Astrid Spannhoff
WB Human 11/19/2010
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate, Sample Amount: 5-15 ul
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit IgG, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA9340

Details

Detection NotesDetection Method: ECL, Exposure Time: 30 seconds, Specific Bands: 17 kDa
  4
reviewed by:
Anonymous
WB Mouse 06/07/2010
View

Summary

ApplicationWestern Blot
Sample TestedCancer Cells
SpeciesMouse
  4
reviewed by:
Hope Lancero
WB Human 05/04/2010
View

Summary

ApplicationWestern Blot
Sample TestedHuman smooth muscle cells, Sample Amount: 30ug
SpeciesHuman
CommentsUsed the product in Western Blots using human whole cell lysates as well as human mitochondrial extracts. The Survivin band appeared after HRP secondary antibody and chemiluminescence.

Blocking

Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: mouse HRP, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#sigma

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 5 minutes

Comments

CommentsUsed the product in Western Blots using human whole cell lysates as well as human mitochondrial extracts. The Survivin band appeared after HRP secondary antibody and chemiluminescence.
  3
reviewed by:
Anonymous
WB Mouse 08/31/2009
View

Summary

ApplicationWestern Blot
Sample Testedmouse cell, Sample Amount: 50ug
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 1% PBS, Blocking Time: 15 minutes, Blocking Temp: 70 degrees Fahrenheit

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/200, Primary Ab Incubation Dilution Buffer: pbs, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: rabbit mAb, Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesDetection Method: dark room, Exposure Time: 1 minute, Specific Bands: 17 kDa
Western Blot Survivin NB500-201
Enlarge
5
reviewed by:
Anonymous
WB Other 07/02/2009
View

Summary

ApplicationWestern Blot
Sample TestedOswald 1771 Abrams D17 Cancer Cell Lines, Sample Amount: 7-15 ug
SpeciesOther
LotR2

Blocking

Blocking DetailsBlocking Buffer: 5% NFDM in 1X TBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/1000, Primary Ab Incubation Dilution Buffer: 1X TBST, Incubation Time: 2hrs, Incubation Temp: 20C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Rabbit Peroxidase, Secondary Ab Dilution Ratio: 1/2000

Details

Detection NotesMethod: Fempto Developer, Exposure Time: 1-5 sec, Pos Ctrl: no transfection to knockdown survivin, Specific Bands: 18 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Survivin Antibody (NB500-201). (Showing 1 - 4 of 4 FAQs).

  1. Can I use this antibody with species other than those listed?
    • The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.
  2. We would like to ensure 3 isoforms of Survivin can all be detected before placing the order. The sample is total protein extracted from Mouse heart. May we ask if it is possible? In addition, as we know, there are 7 isoforms of Human Survivin. May we also ask the reason why there is only one band observed by HeLa (as shown on the product page)?
    • This antibody was made to the full length canonical sequence for Survivin but it has not been tested for reactivity with all isoforms of Survivin. It is possible being a polyclonal but we cannot guarantee it will react with a particular isoform.
  3. I would appreciate if you are able to confirm how many of the splice variants this antibody will detect (survivin wild type, survivin isoform 2 and also survivin 2-alpha). Has this been tested? If you have any data to show this, it would be beneficial.
    • NB500-201 was raised against the full-length survivin protein and is a polyclonal antibody, therefore we do not know where or how many sites the antibody detects. We have not tested for reactivity against specific splice variants, but any customer feedback regarding this would be greatly appreciated.
  4. I hope you can help with a quick query: apologies if I have missed this information on the technical data sheet, but where on the protein has the epitope for antibody been raised?
    • Our Survivin antibody NB500-201 was raised against the full length human survivin protein (UniProt# O15392). We unfortunately do not epitope map our antibodies, so the exact epitope recognized by this antibody has not been determined.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-201AF350
Alexa Fluor 405 NB500-201AF405
Alexa Fluor 488 NB500-201AF488
Alexa Fluor 532 NB500-201AF532
Alexa Fluor 594 NB500-201AF594
Alexa Fluor 647 NB500-201AF647
Alexa Fluor 700 NB500-201AF700
Alexa Fluor 750 NB500-201AF750
Biotin NB500-201B
DyLight 350 NB500-201UV
DyLight 405 NB500-201V
DyLight 488 NB500-201G
DyLight 550 NB500-201R
DyLight 594 NB500-201DL594
DyLight 650 NB500-201C
DyLight 680 NB500-201FR
DyLight 755 NB500-201IR
FITC NB500-201F
HRP NB500-201H
Janelia Fluor 549 NB500-201JF549
Janelia Fluor 646 NB500-201JF646

Additional Survivin Products

Bioinformatics Tool for Survivin Antibody (NB500-201)

Discover related pathways, diseases and genes to Survivin Antibody (NB500-201). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Survivin Antibody (NB500-201)

Discover more about diseases related to Survivin Antibody (NB500-201).
 

Pathways for Survivin Antibody (NB500-201)

View related products by pathway.

PTMs for Survivin Antibody (NB500-201)

Learn more about PTMs related to Survivin Antibody (NB500-201).
 

Research Areas for Survivin Antibody (NB500-201)

Find related products by research area.

Blogs on Survivin. Showing 1-10 of 15 blog posts - Show all blog posts.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Survivin - an inhibitor of apoptosis that drives tumorigenesis and metastasis
Apoptosis is the tightly regulated process of programmed cell death. It plays an important role in normal physiologic development but has also been implicated in a number of diseases. Apoptosis is constantly downregulated by a family of anti-apop...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

Survivin: As long as I know how to live I know I will
Survivin is an anti-apoptotic protein from a large family with related members such as X-linked IAP, cIAP1 and cIAP2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates fundamental physiological events including the cell cycle, feta...  Read full blog post.

Survivin: Infographic
Survivin is involved in promoting cell proliferation and is an inhibitor of apoptosis. Survivin has a critical role in cancer proliferation and neural development. It may have an impact on neural cell proliferative responses following brain injury.L...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.4
5
11
4
8
3
2
2
0
1
0

Anonymous
05/17/2019
Application: WB
Species: Mouse

 
Anna Herman-Antosiewicz
05/04/2017
Application: WB
Species: Mouse and Human

Anonymous
01/11/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol BIRC5
Entrez
Uniprot