p53 Antibody [Unconjugated]

Images

 
Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF ...read more
p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) at 1.7 µg/mL for 3 hours at ...read more
MCF‑7 human breast cancer cell line treated with 300 nM camptothecin overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. p53/DNA complexes were immunoprecipitated ...read more
Simple Western lane view shows lysates of CEM humanT-lymphoblastoid cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p53 at approximately 59 kDa (as ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IP, ChIP, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

p53 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637
Specificity
Detects human, mouse, and rat p53.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TP53
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunoprecipitation 1-2 ug/500 ug cell lysate
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF1355 in the following applications:

Publications
Read Publications using
AF1355 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p53 Antibody [Unconjugated]

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p53 Antibody (AF1355)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(10)
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Filter By Species
Human
(7)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF1355 Applications Species
ZP You, MJ Yu, YL Zhang, K Shi Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll?like receptor?4?NADPH oxidase 4 signaling pathway Mol Med Rep, 2018;0(0):. 2018 [PMID: 30431085] (Western Blot, Mouse) Western Blot Mouse
J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma Oncol Lett, 2018;15(6):9009-9016. 2018 [PMID: 29844818] (Western Blot, Human) Western Blot Human
M Conde, S Michen, R Wiedemuth, B Klink, E Schröck, G Schackert, A Temme Chromosomal instability induced by increased BIRC5/Survivin levels affects tumorigenicity of glioma cells BMC Cancer, 2017;17(1):889. 2017 [PMID: 29282022] (Western Blot, Human) Western Blot Human
D Lessel, D Wu, C Trujillo, T Ramezani, I Lessel, MK Alwasiyah, B Saha, FM Hisama, K Rading, I Goebel, P Schütz, G Speit, J Högel, H Thiele, G Nürnberg, P Nürnberg, M Hammerschm, Y Zhu, DR Tong, C Katz, GM Martin, J Oshima, C Prives, C Kubisch Dysfunction of the MDM2/p53 axis is linked to premature aging J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28846075] (Western Blot, Human) Western Blot Human
Katrien Van Roosbr Combining anti-miR-155 with chemotherapy for the treatment of lung cancers Clin. Cancer Res, 2016;0(0):. 2016 [PMID: 27903673] (Western Blot, Human) Western Blot Human
Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27226307] (Western Blot, Human) Western Blot Human
Wiedemuth R, Klink B, Topfer K, Schrock E, Schackert G, Tatsuka M, Temme A Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53. Mol Cancer, 2014;13(0):107. 2014 [PMID: 24886358] (Western Blot, Human) Western Blot Human
Tsoporis JN, Izhar S, Parker TG Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha. J. Biol. Chem., 2008;283(44):30174-83. 2008 [PMID: 18753141] (Western Blot, Rat) Western Blot Rat
Ishida T, Sakaguchi I, Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon. Biol. Pharm. Bull., 2007;30(5):928-34. 2007 [PMID: 17473437] (Western Blot, Human) Western Blot Human
Davidsen ML, Wurtz SØ, Romer MU, Sorensen NM, Johansen SK, Christensen IJ, Larsen JK, Offenberg H, Brunner N, Lademann U TIMP-1 gene deficiency increases tumour cell sensitivity to chemotherapy-induced apoptosis. Br. J. Cancer, 2006;95(8):1114-20. 2006 [PMID: 17047657] (Western Blot, Mouse) Western Blot Mouse

Review for p53 Antibody (AF1355) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1355:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24886358
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24886358

Details

Detection NotesSee PMID 24886358

Product General Protocols

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Video Protocols

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FAQs for p53 Antibody (AF1355) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose GAF1355
Biotin BAF1355
HRP (Horseradish Peroxidase) HAF1355

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Bioinformatics Tool for p53 Antibody (AF1355)

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Diseases for p53 Antibody (AF1355)

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Pathways for p53 Antibody (AF1355)

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Blogs on p53. Showing 1-10 of 19 blog posts - Show all blog posts.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.

Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

Showing 1-10 of 19 blog posts - Show all blog posts.
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Recent Reviews

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Anonymous
01/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol TP53
Uniprot