Donkey anti-Mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4Monoclonal Antibody (Catalog # MAB41642)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # HAF018). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Donkey
• Conjugate: HRP (Horseradish Peroxidase)

Donkey anti-Mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

• Immunogen: Mouse IgG
• Specificity: Detects mouse IgG₁, IgG_2A, IgG_2B, and IgG₃ heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Donkey
• Gene: IGHG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (HAF018)(13)

Publications using HAF018

• MN Akhtar, S Kumar, P Sen Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 Receptor MBio, 2022;13(4):e0330921. 2022 [PMID: 35924848] (Western Blot, Mouse)
• A Tarar, EM Alyami, CA Peng Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System Methods and protocols, 2020;3(4):. 2020 [PMID: 33271819] (Western Blot, N/A)
• A Singhal, ES Krystofiak, WG Jerome, B Song 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy Sci Rep, 2020;10(1):8663. 2020 [PMID: 32457374] (Western Blot, Human)
• E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia Blood Adv, 2018;2(7):754-761. 2018 [PMID: 29599195] (ELISA Capture, Mouse)
• MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity Nat Commun, 2017;8(1):552. 2017 [PMID: 28916756] (Western Blot, Human)
• R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study Cell Death Dis, 2017;8(8):e3017. 2017 [PMID: 28837142] (Western Blot, Human)
• Y Xia, M Sun, Y Xie, R Shu mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress Oxid Med Cell Longev, 2017;2017(0):6292630. 2017 [PMID: 28804534] (Western Blot, Human)
• P Bokiniec, S Shahbazian, SJ McDougall, BA Berning, D Cheng, IJ Llewellyn-, PG Burke, S McMullan, M Mühlenhoff, H Hildebrand, F Braet, M Connor, NH Packer, AK Goodchild Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract J. Neurosci., 2017;0(0):. 2017 [PMID: 28576943] (Functional Assay, Rat)
• T Poobalasin, LL Yates, SA Walker, M Pereira, NY Gross, A Ali, M Kolatsi-Jo, MR Jarvelin, J Pekkanen, E Papakrivop, DA Long, M Griffiths, D Wagner, M Königshoff, M Hind, C Minelli, CM Lloyd, CH Dean Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair Dis Model Mech, 2017;0(0):. 2017 [PMID: 28237967] (Western Blot, Mouse)
• Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target Sci Rep, 2016;6(0):37360. 2016 [PMID: 27853274] (Western Blot, Human)
• Lynam-Lennon , Niamh, Maher , Stephen, Maguire , Aoife, Phelan , James, Muldoon , Cian, Reynolds , John V, O'Sullivan , Jacintha Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma. PLoS ONE, 2014;9(6):e100738. 2014 [PMID: 24968221] (Western Blot, Human)
• Krishnatry AS, Brazeau DA, Fung HL Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin. Nitric Oxide, 2010;22(1):11-7. 2010 [PMID: 19836459] (Western Blot, Human)
• Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W Sleeping beauty transposition from nonintegrating lentivirus. Mol. Ther., 2009;17(7):1197-204. 2009 [PMID: 19417741] (Western Blot, Human)

Review for IgG Antibody (HAF018) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: Louise Speed WB Pig 08/14/2019

Summary

• Application: Western Blot
• Sample Tested: Muscle tissue
• Species: Pig
• Lot: WVA091611

Bioinformatics

• Gene Symbol: IGHG1