Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

Images

 
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) [NBP1-74822] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP
Format
Pre-absorbed
Concentration
LYOPH

Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Purified Goat IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on goat IgG and light chains on all goat immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, mouse, rabbit, rat IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP1-74822 in the following applications:

Reactivity Notes

Based on IEP, this antibody reacts with and heavy (gamma) chains on goat IgG and light chains on all goat immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C. After reconstitution, dilute with 50% glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates.

Notes

Purity > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-74822) (0)

There are no publications for IgG (H+L) Antibody (NBP1-74822).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for IgG (H+L) Antibody (NBP1-74822) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP1-74822:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IgG (H+L) NBP1-74822
Enlarge
reviewed by:
Paula Aliberti
IHC-P Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman testis
SpeciesHuman
Lot41-121-030117

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-74822) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG (H+L) Products

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Paula Aliberti
10/09/2017
Application: IHC-P
Species: Human