Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of MCF-7 cell line.
Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).
Flow Cytometry: p53 Antibody (BP53-12) [NBP2-29453] - analysis of HT-29 cells using mouse Monoclonal p53 antibody (Orange) and Isotype control Antibody (Blue).
Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution ...read more
Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.
Hu, Ca, Ch, Ha, Mk, Mu(-), Rt(-)Species Glossary
WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P, IP
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Full-length recombinant human p53 was used as immunogen for this antibody.
The epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein.
This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.
Protein G purified
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Human Chromosome Location: 17p13.1 Use in Immunoprecipitation has been reported in literature. In Simple Western only 10-15 uL of the recommended dilution is used per data point. Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-29453 in the following applications:
Nuclear protein p53 plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutations in the evolutionarily conserved codons of the p53 tumor suppressor gene are common in diverse types of human cancer, which includes cancer of colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues. p53 maps to the 17p13.1 region of the human chromosome. Consistent with its role as a tumor suppressor gene in a wide variety of tissue types, p53 performs several different critical functions in regulating cellular growth, replication, and death. p53 positively regulates transcription by binding to specific DNA consensus sequences. These sequences are associated with several known genes including the human ribosomal gene cluster, muscle creatine kinase gene, WAF-1/CIPI/p21, and cyclin G. This sequence-specific transcriptional activation is associated with the induction of growth suppression. p53 also negatively regulates transcription of genes which have TATA box initiated promoters, likely by binding to protein components of the basal transcription machinery. Possibly through the general mechanism of transcriptional regulation, p53 affects cell behavior via several specific pathways. Wild-type p53 suppresses cell proliferation by blocking the transition from G1 to S-phase. This may occur through p53 mediated induction of a universal inhibitor of cyclin-dependent kinases, WAF-1 or CIPI. Increased expression of WAF 1/CIPI/p21 serves to inhibit phosphorylation of Rb protein by cdk complexes, resulting in failure to progress from G1 to S phase. This p53 antibody recognizes a 53kDa protein, which is identified as p53 suppressor gene product. The antibody reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
PRMT6: One Function, Many Roles Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P... Read full blog post.
p14 ARF is an Important Tumor Suppressor The p14ARF (Alternative Reading Frame) tumor suppressor is a protein product of the alternative reading frame (ARF) of the human INK4a locus which regulates a series of cell cycle regulatory proteins to promote cell cycle arrest in response to abnorma... Read full blog post.