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Scientific Resources

ICC Handbook

Learn about multicolor ICC/IF’s conceptual basics, protocols and troubleshooting.

IHC Handbook

Learn the basics of IHC, follow standard protocols, plus troubleshoot technical issues.

Apoptosis Handbook

Read about the process of apoptosis and methods to detect key apoptotic events.

dr Cecconi Novus faculty interview
Faculty Interviews: Fulvio Reggiori, and this is why I research.
Fulvio Reggiori is one of Autophagy’s leading scientists. Read his interview to learn more about his research.

direct vs indirect
Understanding direct vs. indirect detection
Learn the benefits and drawbacks of direct and indirect detection to help you make the right choice for your experiment.

Using blocking peptides
Using blocking peptides to assess antibody specificity.
Read more about blocking peptides and how you can use them as a method to assess antibody specificity.

Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling and plasma membrane breakdown. While these two forms of cell death are clearly distinct, substantial crosstalk occurs between them. ...

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Immunocytochemistry/immunofluorescence (ICC/IF) involves visualization of antigens in cultured or smeared cells with the use of fluorochrome-labeled antibodies. Various combinations of fluorochrome-labeled antibodies make it possible to simultaneously detect multiple antigens in the same sample. However, every experiment involving multiplex or multicolor ICC/IF requires selection of an...

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