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Epigenetic Regulation of Stem Cell Differentiation

Lifetime Achievement Award:

Karen Padgett

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Karen Padgett wins CiteAb Lifetime Achievement Award

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Scientific Resources

Western Blot Handbook

Learn how to perform and troubleshoot a standard western blot protocol.

IHC Handbook

Learn the basics of IHC, follow standard protocols, plus troubleshoot technical issues.

Loading Control Handbook

Get technical guidance on loading control proteins and western blot normalization.

dr Cecconi Novus faculty interview
Faculty Interviews: I’m Francesco Cecconi, and this is why I research.
Francesco Cecconi is one of Autophagy’s leading scientists. Read his interview to learn more about his research.


direct vs indirect
Understanding direct vs. indirect detection
Learn the benefits and drawbacks of direct and indirect detection to help you make the right choice for your experiment.


Using blocking peptides
Using blocking peptides to assess antibody specificity.
Read more about blocking peptides and how you can use them as a method to assess antibody specificity.


Eukaryotic translation initiation factor 4E binding protein 1, or 4EBP1, is an mRNA translational repressor protein that negatively regulates eukaryotic translation initiation factor 4E, or EIF4E.  EIF4E is a protein that forms a complex necessary to block the 5’ ends of mRNA with a 7-methyl-guanosine five-prime cap structure, which is...

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Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two isoforms that are highly and ubiquitously expressed in the cell.  For this reason, measuring beta-actin levels has served as a useful...

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