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Lifetime Achievement Award:

Karen Padgett

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Karen Padgett wins CiteAb Lifetime Achievement Award

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Scientific Resources

Western Blot Handbook

Learn how to perform and troubleshoot a standard western blot protocol.

IHC Handbook

Learn the basics of IHC, follow standard protocols, plus troubleshoot technical issues.

Apoptosis Handbook

Read about the process of apoptosis and methods to detect key apoptotic events.

dr Cecconi Novus faculty interview
Faculty Interviews: I’m Francesco Cecconi, and this is why I research.
Francesco Cecconi is one of Autophagy’s leading scientists. Read his interview to learn more about his research.


direct vs indirect
Understanding direct vs. indirect detection
Learn the benefits and drawbacks of direct and indirect detection to help you make the right choice for your experiment.


Using blocking peptides
Using blocking peptides to assess antibody specificity.
Read more about blocking peptides and how you can use them as a method to assess antibody specificity.


Apoptosis is a form of programmed cell death which is mediated by cysteine proteases called caspases. It is an essential phenomenon in the maintenance of homeostasis and growth of tissues, and it also plays a critical role in immune response. The cytomorphological alterations and the key features of apoptosis are listed below:

...

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Signal amplification methods are widely used in immunohistochemistry (IHC) for detection of rare epitopes and low abundance antigens. While many of these techniques such as the avidin-biotin complex (ABC) method improve staining, they frequently require additional steps and result in higher background staining.  Blocking endogenous biotin, a requirement of using ABC reagents, may not...

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