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Scientific Resources

ICC Handbook

Novus western blot handbook
Learn about multicolor ICC/IF’s conceptual basics, protocols and troubleshooting.
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IHC Handbook

IHC Handbooks
Learn the basics of IHC, follow standard protocols, plus troubleshoot technical issues.
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Apoptosis Handbook

Apoptosis Handbook
Read about the process of apoptosis and methods to detect key apoptotic events.
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Ivan Dikic Novus faculty interview
Faculty Interviews: Ivan Dikic, and this is why I research.
Ivan Dikic has made groundbreaking discoveries in ubiquitin signaling & selective autophagy. Learn more about his research.


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direct vs indirect
Understanding direct vs. indirect detection
Learn the benefits and drawbacks of direct and indirect detection to help you make the right choice for your experiment.


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Using blocking peptides
Using blocking peptides to assess antibody specificity.
Read more about blocking peptides and how you can use them as a method to assess antibody specificity.


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Applications Guide: How to choose fluorophore...

Flow cytometry is an experimental method that was developed to label and examine a high volume of cells in an extremely rapid rate using antibodies conjugated to fluorophores.  The basic concept of flow cytometry is that a cell suspension is situated into a single stream, which then passes through a light source that uses detectors to generate data sets based off cellular properties. ...

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Make the most of your membrane: PVDF vs....

The Western Blot – a tried and true experimental protocol where protein structures are separated via molecular weight/charge and transferred to a membrane before visualization by a chemiluminescent solution (say that three times fast!). Seems simple, right?  While the step-by-step process of a western blot has for the most part remained the same over the years, variations in solutions,...

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