Scientific Resources

Western Blot Handbook

Learn how to perform and troubleshoot a standard western blot protocol.

IHC Handbook

Learn the basics of IHC, follow standard protocols, plus troubleshoot technical issues.

Loading Control Handbook

Get technical guidance on loading control proteins and western blot normalization.

dr Cecconi Novus faculty interview
Faculty Interviews: I’m Francesco Cecconi, and this is why I research.
Francesco Cecconi is one of Autophagy’s leading scientists. Read his interview to learn more about his research.


direct vs indirect
Understanding direct vs. indirect detection
Learn the benefits and drawbacks of direct and indirect detection to help you make the right choice for your experiment.


Using blocking peptides
Using blocking peptides to assess antibody specificity.
Read more about blocking peptides and how you can use them as a method to assess antibody specificity.


Immunohistochemistry (IHC) is a widely applied experimental method used to examine tissue antigen expression and behavior with the use of an antibody conjugated to a secondary tag for visualization.  IHC consists of a tissue preparation phase, an antibody-staining phase, and a result analysis phase - all of which may lead to skewed results if...

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p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is functional or mutated.  The most common mutation in the p53 protein that leads to lack of tumor suppression activity is a missense mutation,...

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