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Scientific Resources

ICC Handbook

Learn about multicolor ICC/IF’s conceptual basics, protocols and troubleshooting.

IHC Handbook

Learn the basics of IHC, follow standard protocols, plus troubleshoot technical issues.

Apoptosis Handbook

Read about the process of apoptosis and methods to detect key apoptotic events.

dr Cecconi Novus faculty interview
Faculty Interviews: I’m Francesco Cecconi, and this is why I research.
Francesco Cecconi is one of Autophagy’s leading scientists. Read his interview to learn more about his research.


direct vs indirect
Understanding direct vs. indirect detection
Learn the benefits and drawbacks of direct and indirect detection to help you make the right choice for your experiment.


Using blocking peptides
Using blocking peptides to assess antibody specificity.
Read more about blocking peptides and how you can use them as a method to assess antibody specificity.


Why: To identify a specific organelle or another cellular structure and to mark individual cells, it is necessary to counterstain them in immunocytochemistry/immunofluorescence (ICC/IF) assays.

How: Counterstaining is often performed with dyes or antibodies specific to the organelle or...

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The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evaded. The MHC complex is broken into two categories, MHC Class I proteins and MHC Class II proteins.  MHC complex I and II proteins are all very...

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