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Secondary Antibodies

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Scientific Resources

ICC Handbook

Learn about multicolor ICC/IF’s conceptual basics, protocols and troubleshooting.

IHC Handbook

Learn the basics of IHC, follow standard protocols, plus troubleshoot technical issues.

Apoptosis Handbook

Read about the process of apoptosis and methods to detect key apoptotic events.

Ivan Dikic Novus faculty interview
Faculty Interviews: Ivan Dikic, and this is why I research.
Ivan Dikic has made groundbreaking discoveries in ubiquitin signaling & selective autophagy. Learn more about his research.

direct vs indirect
Understanding direct vs. indirect detection
Learn the benefits and drawbacks of direct and indirect detection to help you make the right choice for your experiment.

Using blocking peptides
Using blocking peptides to assess antibody specificity.
Read more about blocking peptides and how you can use them as a method to assess antibody specificity.

Flow cytometry is an experimental method that was developed to label and examine a high volume of cells in an extremely rapid rate using antibodies conjugated to fluorophores.  The basic concept of flow cytometry is that a cell suspension is situated into a single stream, which then passes through a light source that uses detectors to generate data sets based off cellular properties. ...

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The Western Blot – a tried and true experimental protocol where protein structures are separated via molecular weight/charge and transferred to a membrane before visualization by a chemiluminescent solution (say that three times fast!). Seems simple, right?  While the step-by-step process of a western blot has for the most part remained the same over the years, variations in solutions,...

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