Goat anti-Rabbit IgG Secondary Antibody [HRP]

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Western Blot: Goat anti-Rabbit IgG Secondary Antibody [HRP] [NBP2-30348H]
Western Blot: Goat anti-Rabbit IgG Secondary Antibody [HRP] [NBP2-30348H] - Analysis of anti-Caspase 1, this antibody, using anti-rabbit HRP at 1:5,000.
Western Blot: Goat anti-Rabbit IgG Secondary Antibody [HRP] [NBP2-30348H]
Goat anti-Rabbit IgG Secondary Antibody [HRP] [NBP2-30348H]

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

Order Details

Goat anti-Rabbit IgG Secondary Antibody [HRP] Summary

Immunogen
Rabbit Ig (H + L)
Specificity
HRP Goat Anti-Rabbit IgG is reported to have minimal crossreactivity with human, mouse or rat serum proteins
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:1000-1:20000
Application Notes
This product does not contain sodium azide. Sodium azide is an inhibitor of peroxidase.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP2-30348H in the following applications:

Publications
Read Publications using
NBP2-30348H in the following applications:

  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Aqueous buffered solution containing BSA
Preservative
ProClin 150
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Rabbit IgG Secondary Antibody [HRP]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NBP2-30348H)(7)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 1 application: WB.


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WB
(6)
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Showing Publications 1 - 7 of 7.
Publications using NBP2-30348H Applications Species
Tarade D, Ma D, Pignanelli C et al. Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest. PLoS ONE. Mar 2 2017 [PMID: 28253265] (WB, Human) WB Human
Ma D, Pignanelli C, Tarade D et al. Cancer Cell Mitochondria Targeting by Pancratistatin Analogs is Dependent on Functional Complex II and III. Sci Rep 2017 Feb 21 [PMID: 28220885] (WB) WB
Song YH, Warncke C, Choi SJ et al. Breast cancer-derived extracellular vesicles stimulate myofibroblast differentiation and pro-angiogenic behavior of adipose stem cells. Matrix Biol. Nov 29 2016 [PMID: 27913195] (WB, Rabbit) WB Rabbit
Martin C, Espaillat MP, Santiago-Schwarz F. IL-10 restricts dendritic cell (DC) growth at the monocyte-to-monocyte-derived DC interface by disrupting anti-apoptotic and cytoprotective autophagic molecular machinery. Immunol. Res. 2015 Sep 22 [PMID: 26395023] (WB) WB
Patil RH, Babu RL, Naveen Kumar M et al. Anti-Inflammatory Effect of Apigenin on LPS-Induced Pro-Inflammatory Mediators and AP-1 Factors in Human Lung Epithelial Cells. Inflammation. 2015 Aug 16 [PMID: 26276128] (WB) WB
Zhang Z, Pang X, Tang Z et al. Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015 Sep [PMID: 25975603]
Patil RH, Babu RL, Naveen Kumar M et al. Apigenin inhibits PMA-induced expression of pro-inflammatory cytokines and AP-1 factors in A549 cells Mol. Cell. Biochem. 2015 Feb 10 [PMID: 25666088] (WB) WB

Review for IgG Antibody (NBP2-30348H) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-30348H:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot IgG NBP2-30348H
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reviewed by:
Arun Satelli
WB Human 10/16/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Cancer cell lysates
SpeciesHuman
CommentsThough suggested dilutions are 1:1000 to 1:5000, 1:1000 is the one that works perfectly.

Primary Anitbody

Dilution Ratio1:1000

Comments

CommentsThough suggested dilutions are 1:1000 to 1:5000, 1:1000 is the one that works perfectly.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (NBP2-30348H) (0)

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Arun Satelli
10/16/2014
Application: WB
Species: Human