Caspase-3 Antibody (31A1067) - (Pro and Active)


Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells more
Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Western Blot Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) more
Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with VP-16. PVDF membrane more
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase 3 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - IHC analysis of a formalin fixed paraffin embedded tissue section of human spleen using 1:200 dilution of Caspase-3 antibody more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using Azide/BSA FREE version of NB100-56708. Analysis of Caspase-3 in A) RAW and B) NIH 3T3 using 32 ug/ml.
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) NB100-56708 (blue) and a matched isotype control more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Lanes 1, 2 and 3 demonstrate the species cross-reactivity of the antibody more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of human breast cancer stained at 4ug/ml. Localization can be more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the azide free version of NB100-56708. Staining of normal colon stained 4ug/ml. Localization can be more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of the human thymus after heat-induced antigen retrieval at 5 more

Product Details

Reactivity Hu, Mu, Rt, Po, ChHa, MaSpecies Glossary
Applications WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
1 mg/ml
Validated by:

Biological Strategies


Caspase-3 Antibody (31A1067) - (Pro and Active) Summary

Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 - 5 ug/ml
  • Simple Western 1:50
  • Electron Microscopy
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • CyTOF-ready
  • Knockout Validated
Application Notes
Useful in Immunohistochemistry-Frozen See Zhang et al., and Immunohistochemistry-Paraffin See Lee et al. Use in Immunocytochemistry/Immunofluorescence was reported in the scientific literature (PMID: 23840553). Use in FLOW cytometry reported in scientific literature ( PMID 27429862). Use in electron microscopy reported in scientific literature ( PMID 27450722).Use in Immunoblotting reported in scientific literature (PMID 28500555).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Caspase-3 Lysate (NBL1-08710)
Caspase-3 Lysate (NBP2-64998)
Reviewed Applications
Read 1 Review rated 4
NB100-56708 in the following applications:

Read Publications using
NB100-56708 in the following applications:

  • EM
    1 publication
  • 3 publications
  • 1 publication
  • IB
    1 publication
  • IHC
    1 publication
  • 2 publications
  • 2 publications
  • WB
    83 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for Caspase-3 Antibody (31A1067) - (Pro and Active)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA


Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (NB100-56708)(113)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chinese Hamster, Mammal.

We have publications tested in 8 applications: EM, FLOW, HEStain, IB, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 113. Show All 113 Publications.
Publications using NB100-56708 Applications Species
Sakchaisri K, Kim SO, Hwang J et al. Anticancer activity of a novel small molecule tubulin inhibitor STK899704. PLoS ONE. 2017 Mar 15 [PMID: 28296906] (WB, Human) WB Human
Nepali K, Lee HY, Lai MJ et al. Ring-opened tetrahydro-gamma-carbolines display cytotoxicity and selectivity with histone deacetylase isoforms. Eur J Med Chem. Dec 21 2016 [PMID: 28038324] (FLOW, WB, Human) FLOW, WB Human
Omar HA Tolba MF Hung JH, Al-Tel TH. OSU-2S/Sorafenib Synergistic Antitumor Combination against Hepatocellular Carcinoma: The Role of PKCd/p53. Front Pharmacol. Dec 14 2016 [PMID: 27965580] (WB, Human) WB Human
Liu X, Lv Z, Zou J et al. Afatinib down-regulates MCL-1 expression through the PERK-eIF2alpha-ATF4 axis and leads to apoptosis in head and neck squamous cell carcinoma Am J Cancer Res Sep 20 2016 [PMID: 27648360] (WB, Human) WB Human
Huang HL, Chao MW, Li YC et al. MPT0G066, a novel anti-mitotic drug, induces JNK-independent mitotic arrest, JNK-mediated apoptosis, and potentiates antineoplastic effect of cisplatin in ovarian cancer Sci Rep Aug 16 2016 [PMID: 27526962] (WB, Human) WB Human
Huang X, Huang R, Gou S et al. Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action Bioconjug. Chem. Aug 15 2016 [PMID: 27494235] (WB, Human) WB Human
Shi P, Oh YT, Zhang G et al. Met gene amplification and protein hyperactivation is a mechanism of resistance to both first and third generation EGFR inhibitors in lung cancer treatment Cancer Lett. 2016 Jul 19 [PMID: 27450722] (EM, FLOW, Human) EM, FLOW Human
Repsold L, Pretorius E, Joubert AM. Ex vivo apoptotic and autophagic influence of an estradiol analogue on platelets Exp Hematol Oncol 2016 Jul 19 [PMID: 27429862] (FLOW, Human) FLOW Human
Chen NC, Chyau CC, Lee YJ et al. Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells Sci Rep Feb 3 2016 [PMID: 26838546] (WB, Human) WB Human
Lai YC, Chuang YC, Chang CP, Yeh TM. Macrophage migration inhibitory factor has a permissive role in concanavalin A-induced cell death of human hepatoma cells through autophagy. Cell Death Dis. 2015 Dec 04 [PMID: 26633714] (WB, Human) WB Human
Show All 113 Publications.

Review for Caspase-3 Antibody (NB100-56708) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56708:
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Images Ratings Applications Species Date Details
reviewed by:
Nayeli Paez-Martinez
IHC-Fr Mouse 04/23/2014



Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB100-56708) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56708AF405
Alexa Fluor 488 Labeled NB100-56708AF488
Alexa Fluor 647 Labeled NB100-56708AF647
Alexa Fluor 700 Labeled NB100-56708AF700
Allophycocyanin Labeled NB100-56708APC
Biotin Labeled NB100-56708B
DyLight 350 Labeled NB100-56708UV
DyLight 405 Labeled NB100-56708V
DyLight 488 Labeled NB100-56708G
DyLight 550 Labeled NB100-56708R
DyLight 650 Labeled NB100-56708C
DyLight 680 Labeled NB100-56708FR
DyLight 755 Labeled NB100-56708IR
HRP Labeled NB100-56708H
PE Labeled NB100-56708PE

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Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Recent Reviews


Nayeli Paez-Martinez
Application: IHC-Fr
Species: Mouse


Gene Symbol CASP3