Caspase-3 Antibody (31A1067) - (Pro and Active)

Images

 
Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with ...read more
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) ...read more
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) ...read more
Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with VP-16. PVDF membrane ...read more
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, ChHa, MaSpecies Glossary
Applications WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
Clone
31A1067
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Caspase-3 Antibody (31A1067) - (Pro and Active) Summary

Immunogen
This Caspase-3 Antibody (31A1067) - (Pro and Active) was developed against full-length recombinant human caspase-3 protein.
Specificity
The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 5 ug/ml
  • Simple Western 1:50
  • Electron Microscopy
  • Flow Cytometry
  • Hematoxylin & Eosin Stain
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • CyTOF-ready
  • Immunohistochemistry Free-Floating
  • Knockdown Validated
  • Knockout Validated
Application Notes
Use in Knockdown Validated reported in scientific literature (PMID:32867814)IHC-Frozen per customer review, IHC-P reported in (PMID:28500555) ICC/IF reported in the scientific literature (PMID: 23840553), Flow cytometry reported in (PMID: 27429862), electron microscopy reported in scientific literature (PMID: 27450722), Immunoblotting reported in scientific literature (PMID: 28500555). Use in Hematoxylin & Eosin Stain reported in scientific literature (PMID: 28186963). Use in Immunohistochemistry free floating reported in scientific literature (PMID: 31771656).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Caspase-3 Overexpression Lysate
Caspase-3 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-56708 in the following applications:

Publications
Read Publications using
NB100-56708 in the following applications:

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-3 Antibody (31A1067) - (Pro and Active)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G.,... Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y.,... Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A.,... Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56708
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Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
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Publications for Caspase-3 Antibody (NB100-56708)(153)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Chinese Hamster, Mammal.

We have publications tested in 12 applications: EM, FLOW, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, KD, WB.


Filter By Application
EM
(1)
FLOW
(3)
Flow
(1)
HEStain
(1)
IB
(1)
ICC/IF
(2)
IHC
(1)
IHC-Fr
(3)
IHC-FrFl
(1)
IHC-P
(2)
KD
(1)
WB
(112)
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Human
(81)
Mouse
(12)
Rat
(6)
Chicken
(1)
Chinese Hamster
(1)
Mammal
(1)
All Species
Showing Publications 1 - 10 of 153. Show All 153 Publications.
Publications using NB100-56708 Applications Species
Xu Y, Sun Q, Yuan F et al. RND2 attenuates apoptosis and autophagy in glioblastoma cells by targeting the p38 MAPK signalling pathway J. Exp. Clin. Cancer Res. Aug 31 2020 [PMID: 32867814] (WB, KD, Human) WB, KD Human
Shrestha S, Adhikary G, Xu W et al. ACTL6A suppresses p21Cip1 expression to enhance the epidermal squamous cell carcinoma phenotype Oncogene Jul 2 2020 [PMID: 32616890] (WB, Human) WB Human
Renema P, Kozhukhar N, Pastukh V et al. Exoenzyme Y induces extracellular active caspase-7 accumulation independent from apoptosis: Modulation of transmissible cytotoxicity Am. J. Physiol. Lung Cell Mol. Physiol. Jun 24 2020 [PMID: 32579398]
Nepali K, Chang TY, Lai MJ et al. Purine/purine isoster based scaffolds as new derivatives of benzamide class of HDAC inhibitors Eur J Med Chem Jun 15 2020 [PMID: 32325365] (WB, Human) WB Human
Wang Y, Guo S, Li D et al. YIPF2 promotes chemotherapeutic agent-mediated apoptosis via enhancing TNFRSF10B recycling to plasma membrane in non-small cell lung cancer cells Cell Death Dis Apr 17 2020 [PMID: 32303681] (WB, Human) WB Human
Xiao X, Sticht C, Yin L et al. Novel plant microRNAs from broccoletti sprouts do not show cross-kingdom regulation of pancreatic cancer Oncotarget Apr 7 2020 [PMID: 32292571] (ICC/IF) ICC/IF
Glauber De Jesus-Silva S, Andreia De Moraes Silva M, Ferraz Carbonel Aa et Al. Heparin attenuates visceral apoptosis in a swine model of hemorrhagic shock and reperfusion injury Ann Vasc Surg Mar 13 2020 [PMID: 32179141]
Wang Y, Su Gf, Huang Zx et Al. Cepharanthine hydrochloride induces mitophagy targeting GPR30 in hepatocellular carcinoma (HCC) Expert Opin. Ther. Targets Apr 1 2020 [PMID: 32106726]
Ye SY, Apple JE, Ren X et al. Microglial VPS35 deficiency regulates microglial polarization and decreases ischemic stroke-induced damage in the cortex J Neuroinflammation Nov 26 2019 [PMID: 31771656] (IHC-FrFl, Mouse) IHC-FrFl Mouse
Huang HL, Liu YM, Sung TY et al. TIMP3 expression associates with prognosis in colorectal cancer and its novel arylsulfonamide inducer, MPT0B390, inhibits tumor growth, metastasis and angiogenesis Theranostics 2019 Sep 18 [PMID: 31588243] (WB, Human) WB Human
Show All 153 Publications.

Review for Caspase-3 Antibody (NB100-56708) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56708:
Filter by Applications
IHC-Fr
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Nayeli Paez-Martinez
IHC-Fr Mouse 04/23/2014
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Summary

ApplicationImmunohistochemistry-Frozen
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB100-56708) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56708AF350
Alexa Fluor 405 NB100-56708AF405
Alexa Fluor 488 NB100-56708AF488
Alexa Fluor 532 NB100-56708AF532
Alexa Fluor 594 NB100-56708AF594
Alexa Fluor 647 NB100-56708AF647
Alexa Fluor 700 NB100-56708AF700
Alexa Fluor 750 NB100-56708AF750
Allophycocyanin NB100-56708APC
Biotin NB100-56708B
DyLight 350 NB100-56708UV
DyLight 405 NB100-56708V
DyLight 488 NB100-56708G
DyLight 550 NB100-56708R
DyLight 594 NB100-56708DL594
DyLight 650 NB100-56708C
DyLight 680 NB100-56708FR
DyLight 755 NB100-56708IR
FITC NB100-56708F
HRP NB100-56708H
Janelia Fluor 549 NB100-56708JF549
Janelia Fluor 646 NB100-56708JF646
PE NB100-56708PE

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The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Nayeli Paez-Martinez
04/23/2014
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CASP3