Caspase-3 Antibody (31A1067) - (Pro and Active)

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Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells ...read more
Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) ...read more
Biological Strategies: Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with VP-16. PVDF membrane ...read more
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase 3 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using Azide/BSA FREE version of NB100-56708. Analysis of Caspase-3 in A) RAW and B) NIH 3T3 using 32 ug/ml.
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) NB100-56708 (blue) and a matched isotype control ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Lanes 1, 2 and 3 demonstrate the species cross-reactivity of the antibody ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of human breast cancer stained at 4ug/ml. Localization can be ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the azide free version of NB100-56708. Staining of normal colon stained 4ug/ml. Localization can be ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of the human thymus after heat-induced antigen retrieval at 5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, ChHa, MaSpecies Glossary
Applications WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
Clone
31A1067
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Caspase-3 Antibody (31A1067) - (Pro and Active) Summary

Immunogen
Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
Specificity
The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 5 ug/ml
  • Simple Western 1:50
  • Electron Microscopy
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • CyTOF-ready
  • Knockout Validated
Application Notes
IHC-Frozen per customer review, IHC-P reported in (PMID:28500555) ICC/IF reported in the scientific literature (PMID: 23840553), Flow cytometry reported in (PMID: 27429862), electron microscopy reported in scientific literature (PMID: 27450722), Immunoblotting reported in scientific literature (PMID: 28500555).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Control
Caspase-3 Lysate (NBL1-08710)
Caspase-3 Lysate (NBP2-64998)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56708 in the following applications:

Publications
Read Publications using
NB100-56708 in the following applications:

  • EM
    1 publication
  • 3 publications
  • 1 publication
  • 1 publication
  • IB
    1 publication
  • IHC
    1 publication
  • 2 publications
  • 2 publications
  • WB
    93 publications

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-3 Antibody (31A1067) - (Pro and Active)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (NB100-56708)(123)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Chinese Hamster, Mammal.

We have publications tested in 9 applications: EM, FLOW, Flow, HEStain, IB, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
EM
(1)
FLOW
(3)
Flow
(1)
HEStain
(1)
IB
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(2)
WB
(93)
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Human
(67)
Mouse
(8)
Rat
(3)
Chicken
(1)
Chinese Hamster
(1)
Mammal
(1)
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Showing Publications 1 - 10 of 123. Show All 123 Publications.
Publications using NB100-56708 Applications Species
Chou CK, Huang HW, Yang CF et al. Reduced camptothecin sensitivity of estrogen receptor-positive human breast cancer cells following exposure to di(2-ethylhexyl)phthalate (DEHP) is associated with DNA methylation changes Environ. Toxicol. Feb 5 2019 [PMID: 30720231] (WB, Human) WB Human
Jaworski S, Strojny B, Sawosz E et al. Degradation of Mitochondria and Oxidative Stress as the Main Mechanism of Toxicity of Pristine Graphene on U87 Glioblastoma Cells and Tumors and HS-5 Cells Int J Mol Sci Feb 2 2019 [PMID: 30717385] (WB, Human) WB Human
Li X, Song H, Kong F et al. Pemetrexed exerts anticancer effects by inducing G0/G1-phase cell cycle arrest and activating the NOXA/Mcl-1 axis in human esophageal squamous cell carcinoma cells. Oncol Lett Feb 1 2019 [PMID: 30675247] (WB, Human) WB Human
Ponandai-Srinivasan S, Lalitkumar PG, Garcia L et al. Mifepristone mediates anti-proliferative effect on ovarian mesenchymal stem/stromal cells from female BRCA1-/ 2- carriers. Acta Obstet Gynecol Scand. Oct 16 2018 [PMID: 30325501] (WB, Human) WB Human
Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. Sep 21 2018 [PMID: 30298003] (WB, Chicken) WB Chicken
Chang YC, Fong Y, Tsai EM et al. Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells.. Int J Mol Sci. Oct 2 2018 [PMID: 30279365] (WB, Human) WB Human
Lin WC, Wang WH, Lin YH et al. Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma bearing mouse model Oncol. Rep. Sep 1 2018 [PMID: 30015952] (WB, Mouse) WB Mouse
Chou Ck, Liu W, Hong YJ et al. Ethyl Acetate Extract of Scindapsus cf. hederaceus Exerts the Inhibitory Bioactivity on Human Non-Small Cell Lung Cancer Cells through Modulating ER Stress. Int J Mol Sci. Jun 21 2018 [PMID: 29933620] (WB, Human) WB Human
Krishnamoorthy V, Khanna R, Parnaik VK. E3 ubiquitin ligase HECW2 targets PCNA and lamin B1 Biochim. Biophys. Acta May 16 2018 [PMID: 29753763] (WB) WB
Qiu S, Wu X, Liao H et al. Pteisolic acid G, a novel ent-kaurane diterpenoid, inhibits viability and induces apoptosis in human colorectal carcinoma cells Oncology Letters 2017 Sep 06 [PMID: 29113182] (Human) Human
Show All 123 Publications.

Review for Caspase-3 Antibody (NB100-56708) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56708:
Filter by Applications
IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Nayeli Paez-Martinez
IHC-Fr Mouse 04/23/2014
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Summary

ApplicationImmunohistochemistry-Frozen
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB100-56708) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56708AF405
Alexa Fluor 488 Labeled NB100-56708AF488
Alexa Fluor 647 Labeled NB100-56708AF647
Alexa Fluor 700 Labeled NB100-56708AF700
Allophycocyanin Labeled NB100-56708APC
Biotin Labeled NB100-56708B
DyLight 350 Labeled NB100-56708UV
DyLight 405 Labeled NB100-56708V
DyLight 488 Labeled NB100-56708G
DyLight 550 Labeled NB100-56708R
DyLight 650 Labeled NB100-56708C
DyLight 680 Labeled NB100-56708FR
DyLight 755 Labeled NB100-56708IR
HRP Labeled NB100-56708H
PE Labeled NB100-56708PE

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Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Nayeli Paez-Martinez
04/23/2014
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CASP3