Caspase-3 Antibody (31A1067) - (Pro and Active)

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with VP-16. PVDF membrane was probed with 0.1 ug/mL of mouse anti-Caspase-3 monoclonal (NB100-56708, Novus Biologicals) followed by 1:2000 dilution donkey anti-mouse IgG.

Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase 3 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep20417), licensed under a CC-BY licence.

Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Lanes 1, 2 and 3 demonstrate the species cross-reactivity of the antibody in human, mouse and rat heart lysate, respectively.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using Azide/BSA FREE version of NB100-56708. Analysis of Caspase-3 in A) RAW and B) NIH 3T3 using 32 ug/ml.

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse anti-Caspase-3 monoclonal (NB100-56708, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of human breast cancer stained at 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis.

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the azide free version of NB100-56708. Staining of normal colon stained 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of the human thymus after heat-induced antigen retrieval at 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Ch, ChHa, Ma
• Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
• Clone: 31A1067
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Caspase-3 Antibody (31A1067) - (Pro and Active) Summary

• Immunogen: Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
• Specificity: The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASP3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 5 ug/ml
  • Simple Western 1:50
  • Electron Microscopy
  • Flow Cytometry
  • Hematoxylin & Eosin Stain
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • CyTOF-ready
  • Knockout Validated
• Application Notes: IHC-Frozen per customer review, IHC-P reported in (PMID:28500555) ICC/IF reported in the scientific literature (PMID: 23840553), Flow cytometry reported in (PMID: 27429862), electron microscopy reported in scientific literature (PMID: 27450722), Immunoblotting reported in scientific literature (PMID: 28500555). Use in Hematoxylin & Eosin Stain reported in scientific literature (PMID: 28186963).
  
  In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
• Control:

  Caspase-3 Overexpression Lysate
  Caspase-3 Knockout HeLa Cell Lysate

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Caspase-3 Antibody (31A1067) - (Pro and Active)

• Apopain
• apoptosis-related cysteine protease
• CASP3
• CASP-3
• caspase 3, apoptosis-related cysteine peptidase
• Caspase3
• Caspase-3
• CPP32
• CPP-32
• CPP32B
• CPP32SREBP cleavage activity 1
• Cysteine protease CPP32
• EC 3.4.22
• EC 3.4.22.56
• LICE-1
• PARP cleavage protease
• procaspase3
• Protein Yama
• SCA-1
• YAMA

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-3 Antibody (NB100-56708)(138)

Publications using NB100-56708

• Huang HL, Liu YM, Sung TY et al. TIMP3 expression associates with prognosis in colorectal cancer and its novel arylsulfonamide inducer, MPT0B390, inhibits tumor growth, metastasis and angiogenesis Theranostics 2019 Sep 18 [PMID: 31588243] (WB, Human)
• Chen, JC;Hsieh, YY;Lo, HL;Li, A;Chou, CJ;Yang, PM; In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells Biomolecules Sep 9 2019 [PMID: 31505885] (WB, Human)
• Kuthati Y, Goutham Davuluri V, Yang C et al. Melatonin MT2 receptor agonist IIK-7 produces antinociception by modulation of ROS and suppression of spinal microglial activation in neuropathic pain rats JPR Aug 1 2019 [PMID: 31496789] (WB, Rat)
• Chang, WT;Wu, CY;Lin, YC;Wu, MT;Su, KL;Yuan, SS;Wang, HD;Fong, Y;Lin, YH;Chiu, CC; C2-Ceramide-Induced Rb-Dominant Senescence-Like Phenotype Leads to Human Breast Cancer MCF-7 Escape from p53-Dependent Cell Death Int J Mol Sci Sep 2 2019 [PMID: 31480728] (WB, Human)
• Sun CM, Enkhjargal B, Reis C et al. Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats CNS Neurosci Ther Aug 22 2019 [PMID: 31436915] (ICC/IF, WB, Rat)
• Nyunt T, Britton M, Wanichthanarak K et al. Mitochondrial oxidative stress-induced transcript variants of ATF3 mediate lipotoxic brain microvascular injury Free Radic. Biol. Med. Jul 26 2019 [PMID: 31356870]
• Mu N, Lei Y, Wang Y et al. Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells Apoptosis Jul 18 2019 [PMID: 31321634] (WB, Human)
• Wang Y, Su G, Huang Z et al. Cepharanthine hydrochloride degrades polyglutamine-expanded androgen receptor proteins through an autophagy pathway in neuron cells Eur. J. Pharmacol. Jul 13 2019 [PMID: 31310754] (WB, Mouse)
• Wu MS, Chien CC, Chang J,Chen YC Pro-apoptotic effect of haem oxygenase-1 in human colorectal carcinoma cells via endoplasmic reticular stress J. Cell. Mol. Med. Jun 14 2019 [PMID: 31199053] (WB, Human)
• Chao MW, Chang LH, Tu HJ et al. Combination treatment strategy for pancreatic cancer involving the novel HDAC inhibitor MPT0E028 with a MEK inhibitor beyond K-Ras status Clin Epigenetics May 29 2019 [PMID: 31142371] (WB, IHC-Fr, Human)
• Zhu Z, Ortiz TS, Mezan S et al. Hydrogen Alleviates Necroptosis and Cognitive Deficits in Lithium-Pilocarpine Model of Status Epilepticus Cell. Mol. Neurobiol. May 14 2019 [PMID: 31089833] (WB, Rat)
• Chen YC, Chien LH, Huang BM, Chia YC. Toona sinensis (aqueous leaf extracts) induces apoptosis through the generation of ROS and activation of intrinsic apoptotic pathways in human renal carcinoma cells. Journal of Functional Foods. 2014 Feb 16 (WB, Human)
  
  Details:
  786-O & A-498 human renal cell carcinoma cells, Fig 5.
• An W, Yao S, Sun X et al. Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells J. Exp. Clin. Cancer Res. May 2 2019 [PMID: 31046799]
• Owonikoko TK, Zhang G, Lallani SB et al. Evaluation of preclinical efficacy of everolimus and pasireotide in thyroid cancer cell lines and xenograft models PLoS ONE Feb 26 2019 [PMID: 30807575] (WB, Human)
• Chou CK, Huang HW, Yang CF et al. Reduced camptothecin sensitivity of estrogen receptor-positive human breast cancer cells following exposure to di(2-ethylhexyl)phthalate (DEHP) is associated with DNA methylation changes Environ. Toxicol. Feb 5 2019 [PMID: 30720231] (WB, Human)
• Jaworski S, Strojny B, Sawosz E et al. Degradation of Mitochondria and Oxidative Stress as the Main Mechanism of Toxicity of Pristine Graphene on U87 Glioblastoma Cells and Tumors and HS-5 Cells Int J Mol Sci Feb 2 2019 [PMID: 30717385] (WB, Human)
• Li X, Song H, Kong F et al. Pemetrexed exerts anticancer effects by inducing G0/G1-phase cell cycle arrest and activating the NOXA/Mcl-1 axis in human esophageal squamous cell carcinoma cells. Oncol Lett Feb 1 2019 [PMID: 30675247] (WB, Human)
• Ponandai-Srinivasan S, Lalitkumar PG, Garcia L et al. Mifepristone mediates anti-proliferative effect on ovarian mesenchymal stem/stromal cells from female BRCA1-/ 2- carriers. Acta Obstet Gynecol Scand. Oct 16 2018 [PMID: 30325501] (WB, Human)
• Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. Sep 21 2018 [PMID: 30298003] (WB, Chicken)
• Chang YC, Fong Y, Tsai EM et al. Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells.. Int J Mol Sci. Oct 2 2018 [PMID: 30279365] (WB, Human)
• Lin WC, Wang WH, Lin YH et al. Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma bearing mouse model Oncol. Rep. Sep 1 2018 [PMID: 30015952] (WB, Mouse)
• Chou Ck, Liu W, Hong YJ et al. Ethyl Acetate Extract of Scindapsus cf. hederaceus Exerts the Inhibitory Bioactivity on Human Non-Small Cell Lung Cancer Cells through Modulating ER Stress. Int J Mol Sci. Jun 21 2018 [PMID: 29933620] (WB, Human)
• Krishnamoorthy V, Khanna R, Parnaik VK. E3 ubiquitin ligase HECW2 targets PCNA and lamin B1 Biochim. Biophys. Acta May 16 2018 [PMID: 29753763] (WB)
• Zhao L, Zhu Z, Yao C et al. VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2 Cell Cycle 2017 Nov 23 [PMID: 29169284] (WB, Mouse)
• Qiu S, Wu X, Liao H et al. Pteisolic acid G, a novel ent-kaurane diterpenoid, inhibits viability and induces apoptosis in human colorectal carcinoma cells Oncology Letters 2017 Sep 06 [PMID: 29113182] (Human)
• Shi P, Oh YT, Deng L et al. Overcoming acquired resistance to AZD9291, a third generation EGFR inhibitor, through modulation of MEK/ERK-dependent Bim and Mcl-1 degradation Clin. Cancer Res. 2017 Aug 01 [PMID: 28765329] (WB, Human)
• Schecher S, Walter B, Falkenstein M et al. Cyclin K dependent regulation of Aurora B affects apoptosis and proliferation by induction of mitotic catastrophe in prostate cancer Int. J. Cancer 2017 Jul 03 [PMID: 28670704] (Human)
• Patel N, Garikapati KR, Pandita RK et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin Sci Rep 2017 Jun 27 [PMID: 28655885] (WB, Human)
• Kao CJ, Chen WF, Guo BL et al. The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity. Int J Mol Sci May 19 2017 [PMID: 28534853] (WB, Human)
• Chang WT, Liu W, Chiu YH et al. A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating a-Tubulin Expression. Molecules May 21 2017 [PMID: 28531143] (WB, Human)
• Wang YY, Chen YK, Hu SC et al. CYT-Rx20 inhibits ovarian cancer cells in vitro and in vivo through oxidative stress-induced DNA damage and cell apoptosis. Cancer Chemother. Pharmacol. Jun 1 2017 [PMID: 28500555] (IB, IHC)
• Chao MW, Chen TH, Huang HL et al. Lanatoside C, a cardiac glycoside, acts through protein kinase Cδ to cause apoptosis of human hepatocellular carcinoma cells. Sci Rep. Apr 7 2017 [PMID: 28387249] (WB, Human)
• Sakchaisri K, Kim SO, Hwang J et al. Anticancer activity of a novel small molecule tubulin inhibitor STK899704. PLoS ONE. 2017 Mar 15 [PMID: 28296906] (WB, Human)
• Chao MW, Huang HL, HuangFu WC et al. An oral quinoline derivative, MPT0B392, causes leukemic cells mitotic arrest and overcomes drug resistant cancer cells. Oncotarget Apr 25 2017 [PMID: 28186963] (WB, HEStain, Human)
• Nepali K, Lee HY, Lai MJ et al. Ring-opened tetrahydro-gamma-carbolines display cytotoxicity and selectivity with histone deacetylase isoforms. Eur J Med Chem. Dec 21 2016 [PMID: 28038324] (FLOW, WB, Human)
• Omar HA Tolba MF Hung JH, Al-Tel TH. OSU-2S/Sorafenib Synergistic Antitumor Combination against Hepatocellular Carcinoma: The Role of PKCd/p53. Front Pharmacol. Dec 14 2016 [PMID: 27965580] (WB, Human)
• Huang YC, Lee CT, Lee JC et al. Epigenetic silencing of miR-137 contributes to early colorectal carcinogenesis by impaired Aurora-A inhibition. Oncotarget. 2016 Nov 22 [PMID: 27764771] (WB, Human)
• Liu X, Lv Z, Zou J et al. Afatinib down-regulates MCL-1 expression through the PERK-eIF2alpha-ATF4 axis and leads to apoptosis in head and neck squamous cell carcinoma Am J Cancer Res Sep 20 2016 [PMID: 27648360] (WB, Human)
• Kwon JJ, Willy JA, Quirin KA et al. Novel role of miR-29a in pancreatic cancer autophagy and its therapeutic potential. Oncotarget. Nov 1 2016 [PMID: 27626694] (WB, Human)
• Patel N, Garikapati KR, Ramaiah MJ et al. miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1 Life Sci. Sep 2 2016 [PMID: 27596816] (WB, Human)
• Ren H, Guo H, Thakur A et al. Blockade efficacy of MEK/ERK-dependent autophagy enhances PI3K/Akt inhibitor NVP-BKM120's therapeutic effectiveness in lung cancer cells. Oncotarget. 2016 Oct 11 [PMID: 27572309] (WB, Human)
• Huang SH, Lien JC, Chen CJ et al. Antiviral Activity of a Novel Compound CW-33 against Japanese Encephalitis Virus through Inhibiting Intracellular Calcium Overload. Int J Mol Sci Aug 27 2016 [PMID: 27563890] (WB, Human)
• Huang HL, Chao MW, Li YC et al. MPT0G066, a novel anti-mitotic drug, induces JNK-independent mitotic arrest, JNK-mediated apoptosis, and potentiates antineoplastic effect of cisplatin in ovarian cancer Sci Rep Aug 16 2016 [PMID: 27526962] (WB, Human)
• Huang X, Huang R, Gou S et al. Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action Bioconjug. Chem. Aug 15 2016 [PMID: 27494235] (WB, Human)
• Shi P, Oh YT, Zhang G et al. Met gene amplification and protein hyperactivation is a mechanism of resistance to both first and third generation EGFR inhibitors in lung cancer treatment Cancer Lett. 2016 Jul 19 [PMID: 27450722] (EM, FLOW, Human)
• Repsold L, Pretorius E, Joubert AM. Ex vivo apoptotic and autophagic influence of an estradiol analogue on platelets Exp Hematol Oncol 2016 Jul 19 [PMID: 27429862] (FLOW, Human)
• Chen NC, Chyau CC, Lee YJ et al. Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells Sci Rep Feb 3 2016 [PMID: 26838546] (WB, Human)
• Lai YC, Chuang YC, Chang CP, Yeh TM. Macrophage migration inhibitory factor has a permissive role in concanavalin A-induced cell death of human hepatoma cells through autophagy. Cell Death Dis. 2015 Dec 04 [PMID: 26633714] (WB, Human)
• Han CK, Chiang HC, Lin CY et al. Comparison of Immunomodulatory and Anticancer Activities in Different Strains of Tremella fuciformis Berk. Am. J. Chin. Med. 2015 Nov 30 [PMID: 26621447] (WB, Human)
• Metzig MO, Fuchs D, Tagscherer KE, Grone HJ. Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-a-dependent necroptosis driven by RIP1 kinase and NF-kB. Oncogene. 2015 Nov 02 [PMID: 26522725] (WB, Human)
• Fassl A, Tagscherer KE, Richter J et al. Inhibition of Notch1 signaling overcomes resistance to the death ligand Trail by specificity protein 1-dependent upregulation of death receptor 5. Cell Death Dis. 2015 Oct 16 [PMID: 26469969] (WB, Human)
  
  Details:
  Caspase 3 antibody was used for WB analysis of lysates from human astrocytes that were transduced with control-AdV or Notch1sh-AdV followed by treatment with TRAIL or 250?nM Staurosporine/STS (Figure 1C).
• Liu X, Guo S, Liu X, Su L. Chaetocin induces endoplasmic reticulum stress response and leads to death receptor 5-dependent apoptosis in human non-small cell lung cancer cells. Apoptosis 2015 Nov 01 [PMID: 26349783] (WB, Human)
• Ge GZ, Xia HJ, He BL et al. Generation and characterization of a breast carcinoma model by PyMT overexpression in mammary epithelial cells of tree shrew, an animal close to primates in evolution. Int. J. Cancer. 2015 Aug 21 [PMID: 26296387] (WB, Mammal)
  
  Details:
  Caspase 3 antibody (Imgenex IMG-144A) was used at 1:1,000 dilution in WB analysis of lysates from mammary tumors of tree shrew (Tupaia belangeri chinensis) that were induced via injection of lentivirus expressing the PyMT oncogene into mammary ducts (Figure 4). Tumors without Bcl-XL expression were found to depict high levels of cleaved Caspase3, an apoptotic biomarker.
• Lu JW, Lin YM, Lai YL et al. MK-2206 induces apoptosis of AML cells and enhances the cytotoxicity of cytarabine Med. Oncol. 2015 Jul 01 [PMID: 26087957] (WB, Human)
  
  Details:
  Caspase-3 antibody was used for WB on lysates of MV4-11 (human, leukemia, acute monocytic cell line) cells that were treated with MK-2206 (specific allosteric AKT inhibitor) in a dose-dependent manner for 24 h (Fig. 3A).
• Li Jiahe, Sharkey Charles C, King Michael R. Piperlongumine and immune cytokine TRAIL synergize to promote tumor death. Scientific Reports 2015 [PMID: 25984950] (WB, Flow, Mouse)
• Jaworski S, Sawosz E, Kutwin M et al. In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma. Int J Nanomedicine. 2015 Mar 11 [PMID: 25759581] (WB, IHC-P, Human)
  
  Details:
  NFkappaB1 antibody used for WB on U87 cells that were treated with graphene oxide (GO) or reduced graphene oxide (rGO) (Figure 7C).
• Roobol A, Roobol J, Bastide A et al. p58(IPK) is an Inhibitor of the eIF2alpha Kinase GCN2 and its Localisation and Expression Underpin Protein Synthesis and ER Processing Capacity. Biochem. J. 2014 Oct 20 [PMID: 25329545] (WB, Chinese Hamster)
  
  Details:
  Caspase-3 (Pro and Active) antibody (clone 31A1067) used for WB assay on CHOK1 cells - stabely expressing p58 (+) or p58 (-) and control cells (WB data in Figure 8B).
• Liu TP, Lo HL, Wei LS et al. S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells. Anticancer Drugs. 2014 Sep 25 [PMID: 25203626] (WB, Human)
  
  Details:
  Caspase-3 antibody used for WB on MDA-MB-231 cells treated or not with doxorubicin, 3-deazaneplanocin A (DZNep; deplete EZH2 protein expression) and GSK343 (directly inhibits enzyme activity through an S-adenosyl-L-methionine-competitive pathway)
• Owonikoko Taofeek K, Zhang Guojing, Deng Xingming et al. Poly (ADP) ribose polymerase enzyme inhibitor, veliparib, potentiates chemotherapy and radiation in vitro and in vivo in small cell lung cancer. Cancer Med. 2014 Dec [PMID: 25124282] (WB)
• Xia M, Meng G, Jiang A et al. Mitophagy switches cell death from apoptosis to necrosis in NSCLC cells treated with oncolytic measles virus. Oncotarget. 2014 Jun 15 [PMID: 25004098] (WB, Human)
  
  Details:
  Caspase-3 antibody used for WB in experiments involving NSCLC cells (A549 and H1299 cells ) treated or not with treated with oncolytic measles virus.
• Yan J, Zhong N, Liu G et al. Usp9x- and Noxa-mediated Mcl-1 downregulation contributes to pemetrexed-induced apoptosis in human non-small-cell lung cancer cells Cell Death Dis. 2014 Jul 04 [PMID: 24991768] (WB, Human)
  
  Details:
  Caspase-3 (Pro and Active) antibody (clone 31A1067) used for WB on human lung cancer cell lines treated or not with Pemetrexed, a folate antimetabolite. Figure 1C - WB on lysates of A549 and H1792 cell lines subjected to treatment with control or Noxa siRNA and/or Pemetrexed. Figure 2C - WB on lysates of A549 and H1792 cell lines subjected to treatment with control or Mcl-1 siRNA and/or Pemetrexed. Figure 3 c & d -WB on lysates of A549 and H1792 cell lines subjected to treatment with control or ATF3/ATF4 siRNA and/or Pemetrexed. Figure 4a -WB on lysates of A549 and H1792 cell lines subjected to treatment with control or Usp9x siRNA and/or Pemetrexed.
• Tsai AC, Wang CY, Liou JP et al. Orally active microtubule-targeting agent, MPT0B271, for the treatment of human non-small cell lung cancer, alone and in combination with erlotinib. Cell Death Dis 2014 Apr 11 [PMID: 24722287] (Human)
  
  Details:
  A549, Fig 4b. Caspase cleavage was induced by MPT0B271 treatment.
• Yi L, Zongyuan Y, Cheng G et al. Quercetin enhances apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in ovarian cancer cells through reactive oxygen species (ROS) mediated CCAAT enhancer-binding protein homologous protein (CHOP)-death receptor 5 pathway. Cancer Sci. 2014 Mar 10 [PMID: 24612139] (WB, Human)
  
  Details:
  SkOV-3, OVAR-3, and TOV-21G ovarian cancer cell lines, Figs 1d, 6b.
• Lin WC, Tsai HF, Liao HJ et al. Helicobacter pylori sensitizes TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human gastric epithelial cells through regulation of FLIP. Cell Death Dis 2014 Mar 07 [PMID: 24603337] (WB, Human)
  
  Details:
  Human gastric epithelial cell lines, Fig 1b. Pro-caspase 3 was detected at 32 kDa. Cleaved caspase-3 was detected at 19 and 17 kDa.
• Chen CH, Chen MC, Wang JC et al. Synergistic Interaction between the HDAC Inhibitor, MPT0E028, and Sorafenib in Liver Cancer Cells In Vitro and In Vivo. Clin. Cancer Res. 2014 Mar 01 [PMID: 24520095] (WB, Human)
  
  Details:
  Hep3B cells: Fig 4A, 6D. Cleaved caspase-3 was detected as two closely spaced bands or doublet.
• Mukherjee S, Chowdhury D, Kotcherlakota R et al. Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System). Theranostics. 2014 Feb 7 [PMID: 24505239] (WB, Mouse)
  
  Details:
  B16 mouse myeloma cells, Fig 9.
• Wang H, Lewsadder M, Dorn E et al. RanBP9 overexpression reduces dendritic arbor and spine density. Neuroscience. 2014 Apr 18 [PMID: 24486966] (Mouse)
• Galvez-Contreras AY, Gonzalez-Castaneda RE, 2 et al. Diphenylhydantoin promotes proliferation in the subventricular zone and dentate gyrus. Am J Neurosci. 2012 Mar 6 [PMID: 24478822]
• Zhao X, Liu X, Su L. Parthenolide induces apoptosis via TNFRSF10B and PMAIP1 pathways in human lung cancer cells. J. Exp. Clin. Cancer Res. 2014 Jan 13 [PMID: 24387758] (WB, Human)
  
  Details:
  A549 human lung cell line and derivatives thereof, Figs 1C-D, 2C, 3C, 4C, 5C-D, 7B, 7D.
• Paul S, Kundu R. Antiproliferative activity of methanolic extracts from two green algae, Enteromorpha intestinalis and Rizoclonium riparium on HeLa cells. Daru 2013 Dec 19 [PMID: 24355313] (WB, Human)
• Aulakh Gurpreet K, Balachandran Yadu, Liu Lixin, Singh Baljit. Angiostatin inhibits activation and migration of neutrophils. Cell Tissue Res. 2014 Feb [PMID: 24297047]
• Hristova Nevyana R, Tagscherer Katrin E, Fassl Anne et al. Notch1-dependent regulation of p27 determines cell fate in colorectal cancer. Int J Oncol. 2013 Dec [PMID: 24141420] (Human)
• Tsai AC, Pai HC, Wang CY et al. In vitro and in vivo anti-tumour effects of MPT0B014, a novel derivative aroylquinoline, and in combination with erlotinib in human non-small-cell lung cancer cells. Br. J. Pharmacol. 2014 Jan 1 [PMID: 24116948] (WB, Human)
  
  Details:
  A549, Fig 4B-C. Both pro and cleaved caspase-3 was detected. The results showed B014 treatment triggered apoptosis and induced caspase-3 cleavage.
• Weissinger Daniel, Tagscherer Katrin E, Macher-Goppinger Stephan et al. The soluble Decoy Receptor 3 is regulated by a PI3K-dependent mechanism and promotes migration and invasion in renal cell carcinoma. Mol Cancer. 2013 [PMID: 24107265] (WB, Human)
• Kant S, Kumar A, Singh SM. Tumor growth retardation and chemosensitizing action of fatty acid synthase inhibitor orlistat on T cell lymphoma: implication of reconstituted tumor microenvironment and multidrug resistance phenotype. Biochim. Biophys. Acta. 2014 Jan 1 [PMID: 24060750] (WB, Human)
  
  Details:
  Daltons human lymphoma ascites tumor model in mice, Fig 3.
• Huang C-K, Yang C-Y, Jeng Y-M et al. Autocrine/paracrine mechanism of interleukin-17B receptor promotes breast tumorigenesis through NF-kappaB-mediated antiapoptotic pathway. Oncogene. 2014 Jun 5 [PMID: 23851503]
• Palavicini JP1, Lloyd BN, Hayes CD et al. RanBP9 Plays a Critical Role in Neonatal Brain Development in Mice. PLoS One 2013 Jun 26 [PMID: 23840553] (IHC-Fr, Mouse)
• Wallace MA, Russell AP. Striated muscle activator of Rho signaling is required for myotube survival but does not influence basal protein synthesis or degradation. Am J Physiol Cell Physiol. 2013 Aug [PMID: 23720020]
• Chiu CC, Haung JW, Chang FR et al. Golden berry-derived 4B-hydroxywithanolide E for selectively killing oral cancer cells by generating ROS, DNA damage, and apoptotic pathways. PLoS One. 2013 May 21 [PMID: 23705007]
• Saha T. LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy. Autophagy. 2012 Nov [PMID: 22874552]
• Hossain A, Radwan FF, Doonan BP et al. A possible cross-talk between autophagy and apoptosis in generating an immune response in melanoma. Apoptosis. 2012 Oct [PMID: 22847295] (WB, Human)
  
  Details:
  WB: human melanoma cell lines (Figs 2A, 5A) and mouse B12 melanoma cells (Fig S3C). The antibody detected both pro and cleaved forms of caspase-3. Cleaved forms were labelled as p14/p17 in Fig 5A.
• Schultz CR, Golembieski WA, King DA et al. Inhibition of HSP27 alone or in combination with pAKT inhibition as therapeutic approaches to target SPARC-induced glioma cell survival. Mol Cancer. 2012 Apr 5 [PMID: 22480225]
• Tagscherer KE, Fassl A, Sinkovic T et al. p53-dependent regulation of Mcl-1 contributes to synergistic cell death by ionizing radiation and the Bcl-2/Bcl-XL inhibitor ABT-737. Apoptosis. 2012 Feb [PMID: 22002102]
• Gonzalez-Perez Oscar, Chavez-Casillas Oscar, Jauregui-Huerta Fernando et al. Stress by noise produces differential effects on the proliferation rate of radial astrocytes and survival of neuroblasts in the adult subgranular zone. Neurosci Res. 2011 Jul [PMID: 21514330] (Mouse)
• Xu D, Meyer F, Ehlers E et al. Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation. J Biol Chem. 2011 May 20 [PMID: 21454650] (WB)
  
  Details:
  Caspase-3 (Pro and Active). WB: IRF-5 overexpressing transformed B cell line, Fig 6C. Note: Caspase-3 cleavage products were visualized in long exposure, Fig 6C.
• Singer M, Trugnan G, Chelbi-Alix MK. Arsenic trioxide reduces 2,4,6-trinitrobenzene sulfonic acid-induced murine colitis via nuclear factor-kB down-regulation and caspase-3 activation. Innate Immun. 2011 Aug [PMID: 20693187] (WB)
  
  Details:
  Caspase-3 Pro and Active (IMG-144A). WB: Total mouse colonic extracts treated with As2O3 and TNBS, Fig 4B. Note: Pro Caspase-3 was seen at 32 kDa and cleaved/active Caspase-3 was seen at 18 kDa, Fig 4B.
• Spassov A, Gredes T, Gedrange T et al. Increased oxidative stress in dystrophin deficient (mdx) mice masticatory muscles. Exp Toxicol Pathol. 2011 Sep [PMID: 20471229]
• Fernandez K, Serinagaoglu Y, Hammond S et al. Mice lacking dystrophin or alpha sarcoglycan spontaneously develop embryonal rhabdomyosarcoma with cancer-associated p53 mutations and alternatively spliced or mutant Mdm2 transcripts. Am J Pathol. 2010 Jan [PMID: 20019182] (WB, Mouse)
  
  Details:
  Caspase-3 Pro and Active (IMG-144A). WB: Mouse skeletal muscle from wild type or mdx mice, Fig 6. Note: Caspase-3 was seen as a doublet at ~36 & ~33 kDa, Fig 6.
• Tsai AC, Pan SL, Sun HL et al. CHM-1, a new vascular targeting agent, induces apoptosis of human umbilical vein endothelial cells via p53-mediated death receptor 5 up-regulation. J Biol Chem. 2010 Feb 19 [PMID: 20007968] (WB, Human)
  
  Details:
  Caspase-3 (IMG-144A): WB (human umbilical vein endothelial cells), Fig 4A, Fig 4B, Fig 5D, Fig 6B.
• Joy B, Sivadasan R, Abraham T E et al. Lysosomal destabilization and cathepsin B contributes for cytochrome c release and caspase activation in embelin-induced apoptosis. Mol Carcinog. 2010 Apr [PMID: 19943316] (WB, Human)
  
  Details:
  Caspase-3 Pro & Active (IMG-144A). WB: Human colon adenocarcinoma (HCT116), Bax knockout, p53 knockout, and p21 knockout cell lines treated with embelin, Fig 4B.
• Temime-Smaali N, Guittat L, Sidibe A et al. The G-quadruplex ligand telomestatin impairs binding of topoisomerase IIIalpha to G-quadruplex-forming oligonucleotides and uncaps telomeres in ALT cells. PLoS One. 2009 Sep 9 [PMID: 19742304]
• Chopra B, Georgopoulos N T, Nicholl A et al. Structurally diverse peroxisome proliferator-activated receptor agonists induce apoptosis in human uro-epithelial cells by a receptor-independent mechanism involving store-operated calcium channels. Cell Prolif. 2009 Oct [PMID: 19614673] (Human)
• Kaneko YS, Nakashima A, Mori K et al. Lipopolysaccharide extends the lifespan of mouse primary-cultured microglia. Brain Res. 2009 Jul 7 [PMID: 19442652] (WB)
  
  Details:
  WB (primary cultures of mouse microglia from the brains of newborn C57BL/6 mice), Fig 4A. A band corresponding to the ~17 kDa fragment of active caspase-3 is seen in the UV-treated cultures along with an ~32 kDa pro-caspase band.
• Louneva Natalia, Cohen Julia W, Han Li-Ying et al. Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol. 2008 Nov [PMID: 18818379] (WB, Human)
• Zou W, Yue P, Khuri FR, Sun SY. Coupling of endoplasmic reticulum stress to CDDO-Me-induced up-regulation of death receptor 5 via a CHOP-dependent mechanism involving JNK activation. Cancer Res. 2008 Sep 15 [PMID: 18794136] (WB, Human)
  
  Details:
  WB (human H1792 cells), Fig. 4B.
• Ray P, De A, Patel M, Gambhir SS. Monitoring caspase-3 activation with a multimodality imaging sensor in living subjects. Clin Cancer Res. 2008 Sep 15 [PMID: 18794090] (WB, Human)
  
  Details:
  WB (human 293T cells), Fig. 2D.
• Hsiao CJ, Ho YF, Hsu JT et al. Mana-Hox displays anticancer activity against prostate cancer cells through tubulin depolymerization and DNA damage stress. Naunyn Schmiedebergs Arch Pharmacol. 2008 Dec [PMID: 18663430] (WB, Human)
  
  Details:
  WB (human prostate cancer cell line PC-3), Fig. 5.
• Raja SM, Chen S, Yue P et al. The natural product honokiol preferentially inhibits cellular FLICE-inhibitory protein and augments death receptor-induced apoptosis. Mol Cancer Ther. 2008 Jul [PMID: 18645030]
  
  Details:
  Refer to figure 2C.
• Chen TH, Pan SL, Guh JH et al. Denbinobin induces apoptosis by apoptosis-inducing factor releasing and DNA damage in human colorectal cancer HCT-116 cells. Naunyn Schmiedebergs Arch Pharmacol. 2008 Nov [PMID: 18607570] (WB, Human)
  
  Details:
  WB (human colorectal cancer cell lines HCT-116 and HT-29), Fig. 3c.
• Chen TH, Pan SL, Guh JH et al. Moscatilin induces apoptosis in human colorectal cancer cells: a crucial role of c-Jun NH2-terminal protein kinase activation caused by tubulin depolymerization and DNA damage. Clin Cancer Res. 2008 Jul 1 [PMID: 18594007] (WB, Human)
  
  Details:
  IMG-144A: WB (human colorectal cancer HCT-116 cells), Fig. 4A,B.
• Lin YD, Chen S, Yue P et al. CAAT/enhancer binding protein homologous protein-dependent death receptor 5 induction is a major component of SHetA2-induced apoptosis in lung cancer cells. Cancer Res. 2008 Jul 1 [PMID: 18593935] (WB)
  
  Details:
  Antibodies cited [WB ( human non-small cell lung cancer cell lines)]: 1. Anti-caspase-3 (IMG-144A): Figs. 1A,B; 5A. 2. DR4 (NT) TRAIL-R1 (IMG-141A): Figs. 3A.
• Yui S, Kanamoto R, Saeki T. Deoxycholic acid can induce apoptosis in the human colon cancer cell line HCT116 in the absence of Bax. Nutr Cancer. 2008 [PMID: 18444140] (WB, Human)
  
  Details:
  IMG-144A: WB (human HCT116 cells), Fig. 3C.
• Campo GM, Avenoso A, Campo S et al. Chondroitin-4-sulphate reduced oxidative injury in caerulein-induced pancreatitis in mice: the involvement of NF-kappaB translocation and apoptosis activation. Exp Biol Med (Maywood). 2008 Jun [PMID: 18408139] (WB)
  
  Details:
  Antbodies cited: 1. Goat Anti-Rabbit HRP Conjugate (20301): ELISA (mouse pancreactic tissue), Fig. 1B 2. Caspase-3 (IMG-144A): WB (mouse pancreatic tissue), Fig. 4B 3. Caspase-7 (IMG-5560): WB (mouse pancreatic tissue), Fig. 5B.
• Liu C, Erlichman C, McDonald C J et al. Heat shock protein inhibitors increase the efficacy of measles virotherapy. Gene Ther. 2008 Jul [PMID: 18356818]
• Wang SW, Pan SL, Huang YC et al. CHM-1, a novel synthetic quinolone with potent and selective antimitotic antitumor activity against human hepatocellular carcinoma in vitro and in vivo. Mol Cancer Ther. 2008 Feb [PMID: 18281518] (WB, Human)
  
  Details:
  1. IMG-144A: WB (Human heptatocellular carcinoma cell line HA22T), Fig. 4A.
• Gdynia G, Lehmann-Koch J, Sieber S et al. BLOC1S2 interacts with the HIPPI protein and sensitizes NCH89 glioblastoma cells to apoptosis. Apoptosis. 2008 Mar [PMID: 18188704] (WB, Human)
  
  Details:
  Refer to figure (human glioblastoma cells), Fig 6A.
• Lee AS, Su MJ. Comparison of the cardiac effects between quinazoline-based alpha1-adrenoceptor antagonists on occlusion-reperfusion injury. J Biomed Sci. 2008 Mar [PMID: 17922254] (WB, Rat)
  
  Details:
  Refer to figure (Rat heart tissue lysates), Fig. 4.
• Shuvaev VV, Tliba S, Nakada M et al. Platelet-endothelial cell adhesion molecule-1-directed endothelial targeting of superoxide dismutase alleviates oxidative stress caused by either extracellular or intracellular superoxide. J Pharmacol Exp Ther. 2007 Nov [PMID: 17712041]
• Lee HS, Cho SB, Lee HE et al. Protein expression profiling and molecular classification of gastric cancer by the tissue array method. Clin Cancer Res. 2007 Jul 15 [PMID: 17634543] (IHC-P)
  
  Details:
  Products cited: 1. Gastric Carcinoma Tissue Microarray (IMH-316): IHC (paraffin), Tables 1,2; Fig.1. 2. Stomach Tissue Microarray (IMH-341), matching normal tissue of IMH-316: IHC (paraffin), Tables 1,2; Fig. 1 3. Caspase-3 (IMG-144A): IHC (paraffin) on g
• Elrod HA, Lin YD, Yue P et al. The alkylphospholipid perifosine induces apoptosis of human lung cancer cells requiring inhibition of Akt and activation of the extrinsic apoptotic pathway. Mol Cancer Ther. 2007 Jul [PMID: 17604333] (WB)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 2BC, Fig 4B, Fig 5B (H157 and H460 cells)].
• Li Ju-Pi, Yang Jia-Ling. Cyclin B1 proteolysis via p38 MAPK signaling participates in G2 checkpoint elicited by arsenite. J Cell Physiol. 2007 Aug [PMID: 17373649] (WB, Human)
• Eiselein L, Wilson DW, Lame MW et al. Lipolysis products from triglyceride-rich lipoproteins increase endothelial permeability, perturb zonula occludens-1 and F-actin, and induce apoptosis. Am J Physiol Heart Circ Physiol. 2007 Jun [PMID: 17259442] (WB)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 5B (T-75 cells)].
• Kumar M A Suresh, Nair Mangalam, Hema P S et al. Pinocembrin triggers Bax-dependent mitochondrial apoptosis in colon cancer cells. Mol Carcinog. 2007 Mar [PMID: 17186548] (Human)
• Chiou SH, Yang YP, Lin JC et al. The immediate early 2 protein of human cytomegalovirus (HCMV) mediates the apoptotic control in HCMV retinitis through up-regulation of the cellular FLICE-inhibitory protein expression. J Immunol. 2006 Nov 1 [PMID: 17056549] (WB)
  
  Details:
  WB, mock or HCMV-infected HRPE cells, Fig 4B.
• Schultze K, B0ck B, Eckert A et al. Troglitazone sensitizes tumor cells to TRAIL-induced apoptosis via down-regulation of FLIP and Survivin. Apoptosis. 2006 Sep [PMID: 16820965] (WB, Human)
  
  Details:
  WB, human glioma and neuroblastoma cells treated with either TRAIL or troglitazone, Fig 2D.
• Chiang PC, Kung FL, Huang DM et al. Induction of Fas clustering and apoptosis by coral prostanoid in human hormone-resistant prostate cancer cells. Eur J Pharmacol. 2006 Aug 7 [PMID: 16806159] (WB, Human)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 4, 6B (human hormone-resistant prostate cancer PC-3 cells)].
• Jarskog LF, Gilmore JH, Glantz LA et al. Caspase-3 activation in rat frontal cortex following treatment with typical and atypical antipsychotics. Neuropsychopharmacology. 2007 Jan [PMID: 16641945] (WB, Rat)
  
  Details:
  Rat frontal cortex tissue: WB (Figs 1a and b, Table 1), IHC (frozen), Fig 3a and b.
• Mohan J, Gandhi AA, Bhavya BC et al. Caspase-2 triggers Bax-Bak-dependent and -independent cell death in colon cancer cells treated with resveratrol. J Biol Chem. 2006 Jun 30 [PMID: 16617056]
  
  Details:
  Caspase-3 (IMG-144) [WB, Fig 1C, Fig 7C, Fig 8 (human colon adenocarcinoma cell line, HCT 116)] 2. AIF (IMG-303) [WB, Fig 2B, IF/FCC, Fig 2B (HCT 116 cells)] 3. RNAi vector (IMG-805) [ Fig 3CD (HCT 116 cells)].
• Huang YT, Pan SL, Guh JH et al. YC-1 suppresses constitutive nuclear factor-kappaB activation and induces apoptosis in human prostate cancer cells. Mol Cancer Ther. 2005 Oct [PMID: 16227413] (WB, Human)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 5A (human prostate cancer PC-3 cells)].
• Jian W, Arora JS, Oe T et al. Induction of endothelial cell apoptosis by lipid hydroperoxide-derived bifunctional electrophiles. Free Radic Biol Med. 2005 Nov 1 [PMID: 16214032] (WB)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 1BD, Fig 3C, Fig 7ABC, Fig 8ABC, (EA.hy 926 endothelial cells)].
• Jin F, Liu X, Zhou Z et al. Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells. Cancer Res. 2005 Jul 15 [PMID: 16024638] (WB)
  
  Details:
  Antibodies cited for WB: 1. Caspase-3 mAb, clone 31A1067 (IMG-144A): Fig 3C, DU145 prostate adenocarcinoma cells. 2. DcR1 (IMG-245-1/IMG-245-2): Fig 6C, DcR1 overexpressing DU145 cells. Note: The specificity of the TRAIL antibody was validated in DcR1 ove
• Chiang PC, Chien CL, Pan SL et al. Induction of endoplasmic reticulum stress and apoptosis by a marine prostanoid in human hepatocellular carcinoma. J Hepatol. 2005 Oct [PMID: 16023761]
• Hylander Bonnie L, Pitoniak Rose, Penetrante Remedios B et al. The anti-tumor effect of Apo2L/TRAIL on patient pancreatic adenocarcinomas grown as xenografts in SCID mice. J Transl Med. 2005 May 19 [PMID: 15943879] (Mouse)
• Lee JC, Lee CH, Su CL et al. Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells. Carcinogenesis. 2005 Oct [PMID: 15905197] (WB)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 3A (HT-29 and HCT 116 cells)].
• Douarre C, Gomez D, Morjani H et al. Overexpression of Bcl-2 is associated with apoptotic resistance to the G-quadruplex ligand 12459 but is not sufficient to confer resistance to long-term senescence. Nucleic Acids Res. 2005 Apr 14 [PMID: 15831792] (WB)
  
  Details:
  1. WB, A549 (human lung carcinoma) cells treated with triazine derivative 12549, Fig 4C. 2. WB, untreated A549, JFD18, JFD9 cells, Fig 3b.
• Tang CH, Hu CC, Wei CW et al. Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells. FEBS Lett. 2005 Jan 3 [PMID: 15620724] (WB)
  
  Details:
  WB; MCF-7, Sk-Hep-1, HL-60 cells treated with RC-RNase, Fig 5.
• Ko CH, Shen SC, Chen YC. Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species. Free Radic Biol Med. 2004 Apr 1 [PMID: 15019974] (WB)
  
  Details:
  Antibodies cited: 1. Caspase-3 (IMG-144A) 2. PARP (IMG-80405) 3. D4-GDI (IMG-396A). [WB, Fig 4AB, 5C, 7A, 8D, 9C, 10 (HL-60 human promyeloleukemic cells)].
• Wu YH, Shih SF, Lin JY. Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3. J Biol Chem. 2004 Apr 30 [PMID: 14960581] (WB)
  
  Details:
  Caspase-3 (IMG-144) [WB, Fig.3C (HeLa cells)].
• Jarskog LF, Selinger ES, Lieberman JA et al. Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation. Am J Psychiatry. 2004 Jan [PMID: 14702258] (WB)
  
  Details:
  Caspase-3 (IMG-144) [WB, Fig 1 ( Postmortem Brain Tissue)].
• Lin HL, Lui WY, Liu TY, Chi CW. Reversal of Taxol resistance in hepatoma by cyclosporin A: involvement of the PI-3 kinase-AKT 1 pathway. Br J Cancer. 2003 Mar 24 [PMID: 12644839] (WB)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 4 (HepG2 cells)].
• Kim KB, Lotan R, Yue P et al. Identification of a novel synthetic triterpenoid, methyl-2-cyano-3,12-dioxooleana-1,9-dien-28-oate, that potently induces caspase-mediated apoptosis in human lung cancer cells. Mol Cancer Ther. 2002 Jan [PMID: 12467212] (WB)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 4C, 4D (H460 and A549 cells)].
• Chen YC, Shen SC, Lee WR et al. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem Pharmacol. 2002 Dec 15 [PMID: 12445860] (WB)
  
  Details:
  Antibodies cited: 1. Caspace-3 (IMG-144A) [WB, Fig 4A, Fig 6A, Fig 8B (HL-60 cells)] 2. PARP (IMG-401A) [WB, Fig 4A, Fig 6A, Fig 8B (HL-60 cells)] 3. D4-GDI (IMG-160A) [WB, Fig 4A, Fig 6A, Fig 8B (HL-60 cells)].
• W R Zhang, K Sato, M Iwai, I Nagano, Y Manabe K Abe. Therapeutic time window of adenovirus-mediated GDNF gene transfer after transient middle cerebral artery occlusion in rat. Brain Research 947, 1140-1145. 2002 [PMID: 12144862] (IHC-Fr, Rat)
  
  Details:
  Antibodies cited: 1. Caspase-3 (IMG-144A) [IHC(Frozen), Fig 2, Table 1 (rat brain)].
• Sun SY, Yue P, Chen X et al. The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells. Cancer Res. 2002 Apr 15 [PMID: 11956107] (WB, Human)
  
  Details:
  Caspase-3 (IMG-144) [WB, Fig 4A,B (H460 human lung cancer cell lines, and NHBE normal lung bronchus cells)].
• Yamaguchi H, Paranawithana SR, Lee MW et al. Epothilone B analogue (BMS-247550)-mediated cytotoxicity through induction of Bax conformational change in human breast cancer cells. Cancer Res. 2002 Jan 15 [PMID: 11809697] (WB)
  
  Details:
  Antibodies cited: 1. Caspase-3 (IMG-144A) [WB, Fig 1D (468 cells)]. 2. D4-GDI (IMG-160A) [WB, Fig 1D (468 cells)].
• Yamaguchi H, Wang HG. The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change. Oncogene. 2001 Nov 22 [PMID: 11753656] (WB)
  
  Details:
  Antibodies cited: 1. Caspase-3 (IMG-144A) [ WB, Fig 2B (FL5.12 cells)]. 2. D4-GDI (IMG-160A) [WB, Fig 2B (FL5.12 cells)].
• Sun SY, Yue P, Kelloff GJ et al. Identification of retinamides that are more potent than N-(4-hydroxyphenyl)retinamide in inhibiting growth and inducing apoptosis of human head and neck and lung cancer cells. Cancer Epidemiol Biomarkers Prev. 2001 Jun [PMID: 11401908] (WB)
  
  Details:
  Caspase-3 (IMG-144A) [WB, Fig 4 AB (H292 cells)].
• Cuddeback SM, Yamaguchi H, Komatsu K et al. Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax. J Biol Chem. 2001 Jun 8 [PMID: 11259440] (WB)
  
  Details:
  Antibodies cited: 1. Caspase-3 (IMG-144A) [ WB, Fig 3C, (FL5.12 cells)] 2. Bif-1 (IMG-265A) [ WB, Fig 8A, (FL5.12 cells)].

Review for Caspase-3 Antibody (NB100-56708) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Nayeli Paez-Martinez IHC-Fr Mouse 04/23/2014

Summary

• Application: Immunohistochemistry-Frozen
• Species: Mouse

Bioinformatics

• Gene Symbol: CASP3