Caspase-3 Antibody (31A1067) - (Pro and Active)

Images

 
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Western Blot Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using Azide/BSA FREE version of NB100-56708. Analysis of Caspase-3 in A) RAW and B) NIH 3T3 using 32 ug/ml.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of the human thymus after heat-induced antigen retrieval at 5 ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Etoposide. PVDF membrane was probed with 0.1 ug/mL ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) ...read more
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Lanes 1, 2 and 3 demonstrate the species cross-reactivity of the antibody ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of human breast cancer stained at 4ug/ml. Localization can be ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the azide free version of NB100-56708. Staining of normal colon stained 4ug/ml. Localization can be ...read more
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase 3 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, MaSpecies Glossary
Applications WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
31A1067
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Caspase-3 Antibody (31A1067) - (Pro and Active) Summary

Immunogen
Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
Specificity
The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 5 ug/ml
  • Simple Western 1:50
  • Electron Microscopy
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • CyTOF-ready
Application Notes
Useful in Immunohistochemistry-Frozen See Zhang et al., and Immunohistochemistry-Paraffin See Lee et al. Use in Immunocytochemistry/Immunofluorescence was reported in the scientific literature (PMID: 23840553). Use in FLOW cytometry reported in scientific literature ( PMID 27429862). Use in electron microscopy reported in scientific literature ( PMID 27450722).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Caspase-3 Lysate (NBL1-08710)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56708 in the following applications:

Publications
Read Publications using
NB100-56708 in the following applications:

  • EM
    1 publication
  • 3 publications
  • 1 publication
  • IB
    1 publication
  • IHC
    1 publication
  • 2 publications
  • 2 publications
  • WB
    83 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-3 Antibody (31A1067) - (Pro and Active)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (NB100-56708)(113)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chinese Hamster, Mammal.

We have publications tested in 8 applications: EM, FLOW, HEStain, IB, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
EM
(1)
FLOW
(3)
HEStain
(1)
IB
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(2)
WB
(83)
All Applications
Filter By Species
Human
(61)
Mouse
(6)
Rat
(3)
Chinese Hamster
(1)
Mammal
(1)
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Showing Publications 1 - 10 of 113. Show All 113 Publications.
Publications using NB100-56708 Applications Species
Qiu S, Wu X, Liao H et al. Pteisolic acid G, a novel ent-kaurane diterpenoid, inhibits viability and induces apoptosis in human colorectal carcinoma cells Oncology Letters 2017 Sep 06 [PMID: 29113182] (Human) Human
Schecher S, Walter B, Falkenstein M et al. Cyclin K dependent regulation of Aurora B affects apoptosis and proliferation by induction of mitotic catastrophe in prostate cancer Int. J. Cancer 2017 Jul 03 [PMID: 28670704] (Human) Human
Patel N, Garikapati KR, Pandita RK et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin Sci Rep 2017 Jun 27 [PMID: 28655885] (WB, Human) WB Human
Kao CJ, Chen WF, Guo BL et al. The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity. Int J Mol Sci May 19 2017 [PMID: 28534853] (WB, Human) WB Human
Chang WT, Liu W, Chiu YH et al. A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating a-Tubulin Expression. Molecules May 21 2017 [PMID: 28531143] (WB, Human) WB Human
Wang YY, Chen YK, Hu SC et al. CYT-Rx20 inhibits ovarian cancer cells in vitro and in vivo through oxidative stress-induced DNA damage and cell apoptosis. Cancer Chemother. Pharmacol. Jun 1 2017 [PMID: 28500555] (IB, IHC) IB, IHC
Chao MW, Chen TH, Huang HL et al. Lanatoside C, a cardiac glycoside, acts through protein kinase Cδ to cause apoptosis of human hepatocellular carcinoma cells. Sci Rep. Apr 7 2017 [PMID: 28387249] (WB, Human) WB Human
Sakchaisri K, Kim SO, Hwang J et al. Anticancer activity of a novel small molecule tubulin inhibitor STK899704. PLoS ONE. 2017 Mar 15 [PMID: 28296906] (WB, Human) WB Human
Chao MW, Huang HL, HuangFu WC et al. An oral quinoline derivative, MPT0B392, causes leukemic cells mitotic arrest and overcomes drug resistant cancer cells. Oncotarget Apr 25 2017 [PMID: 28186963] (WB, HEStain, Human) WB, HEStain Human
Nepali K, Lee HY, Lai MJ et al. Ring-opened tetrahydro-gamma-carbolines display cytotoxicity and selectivity with histone deacetylase isoforms. Eur J Med Chem. Dec 21 2016 [PMID: 28038324] (FLOW, WB, Human) FLOW, WB Human
Show All 113 Publications.

Review for Caspase-3 Antibody (NB100-56708) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NB100-56708:
Filter by Applications
IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Nayeli Paez-Martinez
IHC-Fr Mouse 04/23/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB100-56708) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56708AF405
Alexa Fluor 488 Labeled NB100-56708AF488
Alexa Fluor 647 Labeled NB100-56708AF647
Alexa Fluor 700 Labeled NB100-56708AF700
Allophycocyanin Labeled NB100-56708APC
Biotin Labeled NB100-56708B
DyLight 350 Labeled NB100-56708UV
DyLight 405 Labeled NB100-56708V
DyLight 488 Labeled NB100-56708G
DyLight 550 Labeled NB100-56708R
DyLight 650 Labeled NB100-56708C
DyLight 680 Labeled NB100-56708FR
DyLight 755 Labeled NB100-56708IR
HRP Labeled NB100-56708H
PE Labeled NB100-56708PE

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Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Recent Reviews

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5
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1
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Nayeli Paez-Martinez
04/23/2014
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CASP3
Entrez