Reactivity | Hu, MuSpecies Glossary |
Applications | WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P |
Clone | 1A10 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 1.0 mg/ml |
Immunogen | Purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406] |
Localization | Cytoplasmic |
Marker | Cytosolic Marker |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | GAPDH |
Purity | Ammonium sulfate precipitation |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Control |
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Reviewed Applications |
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Publications |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.03% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Ammonium sulfate precipitation |
Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||||||||
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reviewed by:
Anonymous |
WB | Human | 12/23/2014 |
Summary
Blocking
Details
Comments
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Enlarge |
reviewed by:
Ranveer Singh |
WB | Human | 06/02/2014 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
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Secondary Antibodies |
Isotype Controls |
Diseases for GAPDH Antibody (NBP1-47339)Discover more about diseases related to GAPDH Antibody (NBP1-47339).
| Pathways for GAPDH Antibody (NBP1-47339)View related products by pathway.
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PTMs for GAPDH Antibody (NBP1-47339)Learn more about PTMs related to GAPDH Antibody (NBP1-47339).
| Research Areas for GAPDH Antibody (NBP1-47339)Find related products by research area.
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
Tips on choosing an ideal loading control antibody for Western Blotting Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post. |
GAPDH - A "housekeeping" gene with diverse functions in cellular homeostasis Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots. However, this... Read full blog post. |
A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
GAPDH: More than a housekeeping gene GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway. Apart from its role in gl... Read full blog post. |
GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase) GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin... Read full blog post. |
Time to Start Actin Like a Reliable 'Housekeeper'! A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti... Read full blog post. |
Beta Actin and GAPDH: The Importance of Loading Controls Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control anti... Read full blog post. |
The GAPDH Antibody in Western blot Assays The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch... Read full blog post. |
New Primers Available for ChIP Procedures Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins. Specifically, the primers are for GAPDH, RPL30, MyoD1, AFM, alpha Satellite, and Satel... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Anonymous 12/23/2014 |
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Application: | WB | |
Species: | Human |
Ranveer Singh 06/02/2014 |
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Application: | WB | |
Species: | Human |