Nanog Antibody [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal ...read more
Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at ...read more
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen ...read more
Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were ...read more
Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Nanog Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376
Specificity
Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NANOG
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 5 Reviews rated 3.8
using
AF1997 in the following applications:

Publications
Read Publications using
AF1997 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nanog Antibody [Unconjugated]

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1997
Species: Hu
Applications: WB, ChIP, ICC/IF

Publications for Nanog Antibody (AF1997)(111)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: ChIP, Differentiation, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(2)
Differentiation
(1)
Flow Cytometry
(2)
ICC
(72)
IHC
(7)
IHC-Fr
(7)
IHC-P
(1)
Immunoprecipitation
(2)
Western Blot
(14)
All Applications
Filter By Species
Human
(103)
Mouse
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 10 of 111. Show All 111 Publications.
Publications using AF1997 Applications Species
J Jang, D Han, M Golkaram, M Audouard, G Liu, D Bridges, S Hellander, A Chialastri, SS Dey, LR Petzold, KS Kosik Control over single-cell distribution of G1 lengths by WNT governs pluripotency PLoS Biol., 2019;17(9):e3000453. 2019 [PMID: 31557150] (ICC, Human) ICC Human
V Cerrada, M García-Lóp, A Moreno-Izq, C Villaverde, O Zurita, MI Martin-Mer, J Arenas, C Ayuso, ME Gallardo Derivation of a human DOA iPSC line, IISHDOi006-A, with a mutation in the ACO2 gene: c.1999G>A; p.Glu667Lys Stem Cell Res, 2019;40(0):101566. 2019 [PMID: 31509793] (ICC, Human) ICC Human
J Tiihonen, M Koskuvi, M Storvik, I Hyötyläine, Y Gao, KA Puttonen, R Giniatulli, E Poguzhelsk, I Ojansuu, O Vaurio, TD Cannon, J Lönnqvist, S Therman, J Suvisaari, J Kaprio, L Cheng, AF Hill, M Lähteenvuo, J Tohka, R Giniatulli, Š Lehtonen, J Koistinaho Sex-specific transcriptional and proteomic signatures in schizophrenia Nat Commun, 2019;10(1):3933. 2019 [PMID: 31477693] (ICC, Human) ICC Human
S Park, JE Yoo, DR Lee, J Jang, MS Cho, DS Kim, DW Kim Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) Stem Cell Res, 2019;39(0):101499. 2019 [PMID: 31352199] (ICC, Human) ICC Human
J Shan, J Shen, M Wu, H Zhou, J Feng, C Yao, Z Yang, Q Ma, Y Luo, Y Wang, C Qian Tcf7l1 acts as a suppressor for the self-renewal of liver cancer stem cells and is regulated by IGF/MEK/ERK signaling independent ofβ-catenin Stem Cells, 2019;0(0):. 2019 [PMID: 31322782] (Western Blot, Human) Western Blot Human
N Kolundzic, P Khurana, L Devito, M Donne, C Hobbs, J Jeriha, XFCC Wong, JAE Common, EB Lane, S Dubrac, R Gruber, M Schmuth, TM Mauro, D Ilic Induced pluripotent stem cell line heterozygous for p.R2447X mutation in filaggrin: KCLi002-A Stem Cell Res, 2019;38(0):101462. 2019 [PMID: 31103941] (ICC, Human) ICC Human
C Calatayud, G Carola, I Fernández-, M Valtorta, S Jiménez-De, M Díaz, J Soriano-Fr, G Cappellett, J García-San, Á Raya, A Consiglio CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons Sci Rep, 2019;9(1):6811. 2019 [PMID: 31048719] (ICC, Human) ICC Human
T Neri, E Hiriart, PP van Vliet, E Faure, RA Norris, B Farhat, B Jagla, J Lefrancois, Y Sugi, T Moore-Morr, S Zaffran, RS Faustino, AC Zambon, JP Desvignes, D Salgado, RA Levine, JL de la Pomp, A Terzic, SM Evans, R Markwald, M Pucéat Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis Nat Commun, 2019;10(1):1929. 2019 [PMID: 31028265] (ICC, Human) ICC Human
N Kolundzic, P Khurana, C Hobbs, M Rogar, S Ropret, H Törmä, D Ilic, M Liovic Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype Stem Cell Res, 2019;37(0):101424. 2019 [PMID: 30933721] (ICC, Human) ICC Human
M Sherman-Sa, H Onallah, A Holth, R Reich, B Davidson SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma Gynecol. Oncol., 2019;0(0):. 2019 [PMID: 30904337] (Western Blot, Human) Western Blot Human
Show All 111 Publications.

Reviews for Nanog Antibody (AF1997) (5) 3.85

Average Rating: 3.8
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Other.

Reviews using AF1997:
Filter by Applications
WB
(2)
IF
(2)
ICC
(1)
All Applications
Filter by Species
Human
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Nanog AF1997
Enlarge
reviewed by:
Anonymous
WB Human 12/02/2018
View

Summary

ApplicationWestern Blot
Sample TestedMCF7
SpeciesHuman
LotKKJ061707
 
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24178749
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24178749

Details

Detection NotesSee PMID 24178749

Comments

CommentsPublished: See PMID 24178749
 
reviewed by:
Anonymous
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23910086
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23910086

Details

Detection NotesSee PMID 23910086

Comments

CommentsPublished: See PMID 23910086
 
reviewed by:
Anonymous
IF Other 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22851508
SpeciesOther

Blocking

Blocking DetailsSee PMID 22851508

Details

Detection NotesSee PMID 22851508

Comments

CommentsPublished: See PMID 22851508
 
reviewed by:
Anonymous
ICC Human 01/06/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23778137
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23778137

Details

Detection NotesSee PMID 23778137

Comments

CommentsPublished: See PMID 23778137

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Nanog Antibody (AF1997) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1997
NL493 NL1997G
Phycoerythrin IC1997P

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Bioinformatics Tool for Nanog Antibody (AF1997)

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Blogs on Nanog.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Recent Reviews

3.8
5
0
4
4
3
1
2
0
1
0

Anonymous
12/02/2018
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol NANOG
Uniprot