beta-Actin Antibody (8H10D10)

Images

 
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, ...read more
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Western blot analysis using anti-Beta Actin mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell ...read more
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Pancreatic cell lines. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (8H10D10) [NBP1-47423] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP1-47423) at ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (8H10D10) [NBP1-47423] - Confocal immunofluorescence analysis of SK-BR-3 (left) and A549 (right) cells using anti-Beta Actin mAb (red, the secondary Ab is ...read more
Simple Western: beta-Actin Antibody (8H10D10) [NBP1-47423] - Simple Western lane view shows a specific band for Beta Actin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Flow Cytometry: beta-Actin Antibody (8H10D10) [NBP1-47423] - analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of Beta Actin expression in 1) HeLa 2) HepG2 and 3) Cos7 whole cell lysates.
Flow Cytometry: beta-Actin Antibody (8H10D10) [NBP1-47423] - Flow cytometric analysis of MCF-7 cells using anti-Beta Actin mAb (right) and negative control (left).

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF
Clone
8H10D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

beta-Actin Antibody (8H10D10) Summary

Immunogen
Synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
Localization
Cytoplasm, cytoskeleton.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 12.5
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Application Notes
This Beta Actin (8H10D10) antibody is useful in Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
NIH 3T3 Lysate (NB800-PC8)
MCF-7 Lysate (NB800-PC5)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-47423 in the following applications:

Publications
Read Publications using
NBP1-47423 in the following applications:

  • IB
    1 publication

Reactivity Notes

Human, mouse, rat, Chinese hamster and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for beta-Actin Antibody (8H10D10)

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Beta Actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because Beta Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to Beta Actin provide a specific and useful tool in studying the intracellular distribution of Beta Actin and the static and dynamic aspects of the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Actin Antibody (NBP1-47423)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IB.


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IB
(1)
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(2)
Mouse
(1)
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Reviews for beta-Actin Antibody (NBP1-47423) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Human and Mouse.
We have 2 reviews tested in 1 application: WB.

Reviews using NBP1-47423:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot beta-Actin NBP1-47423
Enlarge
reviewed by:
Anonymous
WB Human and Mouse 11/25/2017
View

Summary

ApplicationWestern Blot
Sample TestedHepG2
SpeciesHuman and Mouse
LotB-4
Western Blot beta-Actin NBP1-47423
Enlarge
reviewed by:
Ming Lu
WB Human 08/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman Pancreatic Cancer Cell line
SpeciesHuman
Lot013013
CommentsSuper clean antibody, very easy to work with.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Novusbio Cat#NB7539
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives strong band at correct MW.

Comments

CommentsSuper clean antibody, very easy to work with.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NBP1-47423). (Showing 1 - 5 of 5 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. What is the immunogen sequence of this Beta-Actin antibody?
    • A synthetic sequence corresponding to N-terminal region of Human beta Actin, conjugate to KLH. The exact sequence is proprietary.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE Labeled NBP1-47423PE

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Bioinformatics Tool for beta-Actin Antibody (NBP1-47423)

Discover related pathways, diseases and genes to beta-Actin Antibody (NBP1-47423). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for beta-Actin Antibody (NBP1-47423)

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Blogs on beta-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
11/25/2017
Application: WB
Species: Human and Mouse

Ming Lu
08/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB
Entrez
Uniprot