Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, ...read more
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis using anti-Beta Actin mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysates.
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Pancreatic cell lines. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (8H10D10) [NBP1-47423] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP1-47423) at ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of SK-BR-3 (left) and A549 (right) cells using anti-Beta Actin mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: ...read more
Simple Western: beta-Actin Antibody (8H10D10) [NBP1-47423] - Image shows a specific band for Beta Actin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Three different human cancer cell lines were probed with the antibody. Image from verified customer review.
Flow Cytometry: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).
Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of Beta Actin expression in 1) HeLa 2) HepG2 and 3) Cos7 whole cell lysates.
Flow Cytometry: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of MCF-7 cells using anti-Beta Actin mAb (right) and negative control (left).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
Ammonium sulfate precipitation
Alternate Names for beta-Actin Antibody (8H10D10)
actin, cytoplasmic 1
beta cytoskeletal actin
PS1TP5-binding protein 1
Beta Actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because Beta Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to Beta Actin provide a specific and useful tool in studying the intracellular distribution of Beta Actin and the static and dynamic aspects of the cytoskeleton.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for beta-Actin Antibody (NBP1-47423). (Showing 1 - 5 of 5 FAQs).
Why is beta actin used as a loading control in blotting ?
Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Are the beta-Actin antibodies validated in Simple Western?
Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Do Beta-Actin antibodies come in lyophilized format?
ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
What the theoretical molecular weight for Beta-Actin antibodies?
The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
What is the immunogen sequence of this Beta-Actin antibody?
A synthetic sequence corresponding to N-terminal region of Human beta Actin, conjugate to KLH. The exact sequence is proprietary.
Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post.
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