Caspase-3 Antibody - (Pro and Active)


Western Blot: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Analysis of Caspase-3. Lysates from Jurkat cells (lane 1), normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas more
Immunohistochemistry-Paraffin: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ using this antibody at more
Immunohistochemistry-Paraffin: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Caspase-3 expression in formalin-fixed, paraffin-embedded human reactive lymph node using NB100-56112 at 1:2000. Staining is seen in more
Simple Western: Caspase-3 Antibody - (Pro and Active) [NB100-56112] - Simple Western lane view shows a specific band for Caspase 3 in 0.2 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions more

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Caspase-3 Antibody - (Pro and Active) Summary

Recombinant full-length human Caspase-3 protein (pro-form) was used as immunogen.
The subcellular location is primarly in the cytoplasm.
Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Whole antisera
0.05% Sodium Azide


  • Western Blot
  • Simple Western 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
NB100-56112 in the following applications:

  • 1 publication
  • WB
    4 publications

Reactivity Notes

Cross reacts with Gerbil.

Alternate Names for Caspase-3 Antibody - (Pro and Active)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA


Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-3 polyclonal antisera recognizes the proform of caspase-3 (approx. 32) kDa), and the large (approx. 14-21 kDa) and small (approx. 10 kDa) subunits of active/cleaved Caspase-3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (NB100-56112)(10)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 2 applications: IHC-P, WB.

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Showing Publications 1 - 10 of 10.
Publications using NB100-56112 Applications Species
Cheng Tc, Lai Cs, Chung Mc et al. Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors PLoS OnE et al. 2014 Nov 13 [PMID:25389774] (WB, Human) WB Human
Samaga KKL, Rao GV, Chandrashekara Reddy G. Synthetic racemates of abyssinone I and II induces apoptosis through mitochondrial pathway in human cervix carcinoma cells. Bioorganic Chemistry. 2014 Jun 26 [PMID:25019692] (WB, Human) WB Human
Krajewska Maryla, You Zerong, Rong Juan et al. Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity. PLoS One. 2011 [PMID:21957448] (WB, Mouse) WB Mouse
Liu Q, Zhao X, Frissora F et al. FTY720 demonstrates promising preclinical activity for chronic lymphocytic leukemia and lymphoblastic leukemia/lymphoma. Blood. 2008 Jan 1 [PMID:17761520]
Zhao X, Lapalombella R, Joshi T et al. Targeting CD37-positive lymphoid malignancies with a novel engineered small modular immunopharmaceutical. Blood. 2007 Oct 1 [PMID:17440052]
Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004 Oct [PMID:15380478] (WB, IHC-P, Mouse, Canine, Rat) WB, IHC-P Mouse, Canine, Rat
Knoblach SM, Nikolaeva M, Huang X et al. Multiple caspases are activated after traumatic brain injury: evidence for involvement in functional outcome. J Neurotrauma. 2002 Oct [PMID:12427325]
Berger T, Kretzler M. Interaction of DAP3 and FADD only after cellular disruption. Nat Immunol. 2002 Jan [PMID:11753396]
Svingen PA, Karp JE, Krajewski S et al. Evaluation of Apaf-1 and procaspases-2, -3, -7, -8, and -9 as potential prognostic markers in acute leukemia. Blood. 2000 Dec 1 [PMID:11090079]
Krajewski S, Gascoyne RD, Zapata JM et al. Immunolocalization of the ICE/Ced-3-family protease, CPP32 (Caspase-3), in non-Hodgkin's lymphomas, chronic lymphocytic leukemias, and reactive lymph nodes. Blood. 1997 May 15 [PMID:9160689]

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Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Gene Symbol CASP3

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