Rabbit anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Western blot shows detection of Goat Anti-Human/Mouse/Rat Syntaxin1A Polyclonal Antibody (Catalog # AF7237)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF017). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

• Reactivity: Gt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP (Horseradish Peroxidase)

Rabbit anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

• Immunogen: Goat IgG
• Specificity: Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rat IgG, chicken IgY and human IgG is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
• Preservative: Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rabbit anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (HAF017)(54)

Publications using HAF017

• K Chan, AG Farias, H Lee, F Guvenc, P Mero, KR Brown, H Ward, M Billmann, K Aulakh, A Astori, S Haider, E Marcon, U Braunschwe, S Pu, A Habsid, AH Yan Tong, N Christie-H, P Budylowski, A Ghalami, S Mubareka, F Maguire, A Banerjee, KL Mossman, J Greenblatt, SD Gray-Owen, B Raught, BJ Blencowe, M Taipale, C Myers, J Moffat Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors Heliyon, 2022;0(0):e12744. 2022 [PMID: 36597481] (Western Blot, Human)
• W Asanprakit, DN Lobo, O Eremin, AJ Bennett M1 macrophages evoke an increase in polymeric immunoglobulin receptor (PIGR) expression in MDA-MB468 breast cancer cells through secretion of interleukin-1beta Scientific Reports, 2022;12(1):16842. 2022 [PMID: 36207349] (Western Blot, Human)
• JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein Inferring and perturbing cell fate regulomes in human brain organoids Nature, 2022;0(0):. 2022 [PMID: 36198796] (Western Blot, Human)
• M Pereira, DF Durso, CE Bryant, EA Kurt-Jones, N Silverman, DT Golenbock, RT Gazzinelli The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways Cell Reports, 2022;40(7):111225. 2022 [PMID: 35977521] (Western Blot, Mouse)
• T Turunen, M Magris, M Malinen, J Kekäläinen Seminal-Plasma-Mediated Effects on Sperm Performance in Humans Cells, 2022;11(14):. 2022 [PMID: 35883590] (Western Blot, Human)
• K Marek, F Armando, VM Nippold, K Rohn, P Plattet, G Brogden, G Gerold, W Baumgärtne, C Puff Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor International Journal of Molecular Sciences, 2022;23(11):. 2022 [PMID: 35682834] (Western Blot, Canine)
• C Morel, A Christophe, K Maguin-Gat, J Paoli, JD Turner, H Schroeder, N Grova Head-to-Head Study of Developmental Neurotoxicity and Resultant Phenotype in Rats: alpha-Hexabromocyclododecane versus Valproic Acid, a Recognized Model of Reference for Autism Spectrum Disorders Toxics, 2022;10(4):. 2022 [PMID: 35448441] (Western Blot, Rat)
• X Gao, X Kang, H Lu, E Xue, R Chen, J Pan, J Ma Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis?fibroblast?like synoviocytes by inhibiting the NF?kappaB and MAPK signaling pathways Molecular Medicine Reports, 2022;25(5):. 2022 [PMID: 35322865] (IF, Rat)
• I Temponeras, G Stamatakis, M Samiotaki, D Georgiadis, H Pratsinis, G Panayotou, E Stratikos ERAP2 Inhibition Induces Cell-Surface Presentation by MOLT-4 Leukemia Cancer Cells of Many Novel and Potentially Antigenic Peptides International Journal of Molecular Sciences, 2022;23(3):. 2022 [PMID: 35163832] (Western Blot, Human)
• C Hu, CA Leche, A Kiyatkin, Z Yu, SE Stayrook, KM Ferguson, MA Lemmon Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias Nature, 2022;602(7897):518-522. 2022 [PMID: 35140400] (Western Blot, Human)
• S Murthy, S Baruah, JL Bowen, K Keck, BA Wagner, GR Buettner, DB Sykes, J Klesney-Ta TREM-1 is required for enhanced OpZ-induced superoxide generation following priming Journal of leukocyte biology, 2022;0(0):. 2022 [PMID: 35075692] (Western Blot, Mouse)
• F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 2021;12(1):7333. 2021 [PMID: 34921145] (IHC, Human)
• Y Deng, X Xia, Y Zhao, Z Zhao, C Martinez, W Yin, J Yao, Q Hang, W Wu, J Zhang, Y Yu, W Xia, F Yao, D Zhao, Y Sun, H Ying, MC Hung, L Ma Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance Nature Communications, 2021;12(1):7041. 2021 [PMID: 34873175] (IHC, Human)
• X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion Nature, 2021;0(0):. 2021 [PMID: 34732895] (Western Blot, Mouse)
• J Hyun, J Im, SW Kim, HY Kim, I Seo, SH Bhang Morus alba Root Extract Induces the Anagen Phase in the Human Hair Follicle Dermal Papilla Cells Pharmaceutics, 2021;13(8):. 2021 [PMID: 34452116] (Western Blot, Human)
• G Galaris, K Montagne, JH Thalgott, GJPE Goujon, S van den Dr, S Martin, HJ Mager, CL Mummery, TJ Rabelink, F Lebrin Thresholds of Endoglin Expression in Endothelial Cells Explains Vascular Etiology in Hereditary Hemorrhagic Telangiectasia Type 1 International Journal of Molecular Sciences, 2021;22(16):. 2021 [PMID: 34445652] (Western Blot, Mouse)
• J Zhou, J Yang, YM Wang, H Ding, TS Li, ZH Liu, L Chen, RQ Jiao, DM Zhang, LD Kong IL-6/STAT3 signaling activation exacerbates high fructose-induced podocyte hypertrophy by ketohexokinase-A-mediated tristetraprolin down-regulation Cellular Signalling, 2021;86(0):110082. 2021 [PMID: 34252535] (Western Blot, Rat)
• C Vetrei, M Passariell, G Froechlich, R Rapuano Le, N Zambrano, C De Lorenzo Immunomodulatory mAbs as Tools to Investigate on Cis-Interaction of PD-1/PD-L1 on Tumor Cells and to Set Up Methods for Early Screening of Safe and Potent Combinatorial Treatments Cancers, 2021;13(12):. 2021 [PMID: 34201082] (Western Blot, Human)
• YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS Aging Cell, 2021;0(0):e13388. 2021 [PMID: 34086398] (Western Blot, Human)
• Y Nagai, H Nakao, A Kojima, Y Komatsubar, Y Ohta, N Kawasaki, N Kawasaki, H Toyoda, T Kawasaki Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies Biomolecules, 2021;11(4):. 2021 [PMID: 33805466] (Western Blot, Human)
• Z Li, Y Cao, Y Li, Y Zhao, X Chen Vaccine delivery alerts innate immune systems for more immunogenic vaccination JCI Insight, 2021;0(0):. 2021 [PMID: 33690222] (Western Blot, Mouse)
• M Ogoyama, A Ohkuchi, H Takahashi, D Zhao, S Matsubara, T Takizawa LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases International Journal of Molecular Sciences, 2021;22(3):. 2021 [PMID: 33513878] (Western Blot, Human)
• KK Oo, T Kamolhan, A Soni, S Thongchot, C Mitrpant, P O-Charoenr, C Thuwajit, P Thuwajit Development of an engineered peptide antagonist against periostin to overcome doxorubicin resistance in breast cancer BMC Cancer, 2021;21(1):65. 2021 [PMID: 33446140] (Western Blot, Human)
• RS Najjar, NS Akhavan, S Pourafshar, BH Arjmandi, RG Feresin Cornus officinalis var. koreana Kitam polyphenol extract decreases pro-inflammatory markers in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing Akt phosphorylation Journal of ethnopharmacology, 2020;0(0):113734. 2020 [PMID: 33359857] (Western Blot, Mouse)
• S Yangngam, S Thongchot, A Pongpaibul, K Vaeteewoot, S Pinlaor, P Thuwajit, S Okada, MA Hermoso, C Thuwajit High level of interleukin-33 in cancer cells and cancer-associated fibroblasts correlates with good prognosis and suppressed migration in cholangiocarcinoma J Cancer, 2020;11(22):6571-6581. 2020 [PMID: 33046978] (Western Blot, Human)
• J Chida, H Hara, K Uchiyama, E Takahashi, H Miyata, H Kosako, Y Tomioka, T Ito, H Horiuchi, H Matsuda, H Kido, S Sakaguchi Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice PLoS Pathog., 2020;16(8):e1008823. 2020 [PMID: 32845931] (Western Blot, Mouse)
• L Tu, X Sun, L Yang, T Zhang, X Zhang, X Li, B Dong, Y Liu, M Yang, L Wang, Y Yu TGF-beta2 interfering oligonucleotides used as adjuvants for microbial vaccines J. Leukoc. Biol., 2020;0(0):. 2020 [PMID: 32794350] (Western Blot, Mouse)
• B Ganesan, S Parameswar, A Sharma, S Krishnakum Clinical relevance of B7H3 expression in retinoblastoma Sci Rep, 2020;10(1):10185. 2020 [PMID: 32576886] (Western Blot, Human)
• X Li, Y Wang, Q Zhu, K Yuan, Z Su, F Ge, RS Ge, Y Huang Epidermal growth factor regulates the development of stem and progenitor Leydig cells in rats J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32441057] (Western Blot, Rat)
• Z Ren, Z Yang, Y Lu, R Zhang, H Yang Anti?glycolipid disorder effect of epigallocatechin?3?gallate on high?fat diet and STZ?induced T2DM in mice Mol Med Rep, 2020;21(6):2475-2483. 2020 [PMID: 32236613] (Western Blot, Mouse)
• S Barbaux, C Ialy-Radio, M Chalbi, E Dybal, M Homps-Legr, M Do Cruzeir, D Vaiman, JP Wolf, A Ziyyat Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion Sci Rep, 2020;10(1):5335. 2020 [PMID: 32210282] (Western Blot, Mouse)
• Alawi L, Emberesh S, Owuor B, Chodavarapu H, Fadnavis R, El-Amouri S, Elased K Effect of hyperglycemia and rosiglitazone on renal and urinary neprilysin in db/db diabetic mice. Physiol Rep, 0;8(3):e14364. 0 [PMID: 32026607] (IHC, Mouse)
• KA Lloyd, BN Parsons, MD Burkitt, AR Moore, S Papoutsopo, M Boyce, CA Duckworth, K Exarchou, N Howes, L Rainbow, Y Fang, C Oxvig, S Dodd, A Varro, N Hall, DM Pritchard Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors Cell Mol Gastroenterol Hepatol, 2020;0(0):. 2020 [PMID: 32004755] (Western Blot, Human)
• IF Scheiber, NO Alarcon, N Zhao Manganese Uptake by A549 Cells is Mediated by Both ZIP8 and ZIP14 Nutrients, 2019;11(7):. 2019 [PMID: 31261654] (Western Blot, Human)
• VB Lê, J Dubois, C Couture, MH Cavanagh, O Uyar, A Pizzorno, M Rosa-Calat, MÈ Hamelin, G Boivin Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice PLoS Pathog., 2019;15(4):e1007689. 2019 [PMID: 30964929] (Western Blot, Human)
• H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine Transl Oncol, 2019;12(3):397-403. 2019 [PMID: 30530187] (ELISA Development, Human)
• T Tamberg, Z Hong, D Schepper, S Skovbjerg, DM Dupont, L Vitved, CR Schar, K Skjoedt, LK Vogel, JK Jensen Blocking the proteolytic activity of zymogen matriptase�with antibody-based inhibitors J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30409910] (Western Blot)
• G Liu, L Chen, Q Cai, H Wu, Z Chen, X Zhang, P Lu Streptozotocin?induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization Mol Med Rep, 2018;0(0):. 2018 [PMID: 30221697] (Mouse)
• EC Bwalya, S Kim, J Fang, HMS Wijekoon, K Hosoya, M Okumura Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes PLoS ONE, 2017;12(5):e0177144. 2017 [PMID: 28472120] (Western Blot, Canine)
• SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma JCI Insight, 2017;2(3):e88297. 2017 [PMID: 28194433] (Western Blot, Mouse)
• Apichart Suksamrarn Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy In Vivo, 2016;30(6):869-877. 2016 [PMID: 27815474] (Western Blot, Rat)
• Ahn J, Shin S, Suh Y, Park J, Hwang S, Lee K Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail. PLoS ONE, 2015;10(4):e0124768. 2015 [PMID: 25867079] (Western Blot, Avian - Quail)
• Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation. PLoS ONE, 2015;10(3):e0119738. 2015 [PMID: 25780921] (Western Blot, Mouse)
• Zhang X, Westerhoff M, Hart J Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up. Mod Pathol, 2015;28(5):654-61. 2015 [PMID: 25412842] (IHC-P, Human)
• Zouk H, D'Hennezel E, Du X, Ounissi-Benkalha H, Piccirillo C, Polychronakos C Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus. Clin Exp Immunol, 2014;175(3):485-97. 2014 [PMID: 24237155] (Western Blot, Human)
• Sebag S, Bastarache J, Ware L Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells. J Biol Chem, 2013;288(11):7875-84. 2013 [PMID: 23362270] (Western Blot, Mouse)
• Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis. Lab Invest, 2012;92(12):1712-25. 2012 [PMID: 23007134] (Western Blot, Mouse)
• Wu Z, Luo H, Thorin E, Tremblay J, Peng J, Lavoie JL, Wang Y, Qi S, Wu T, Wu J Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure. J. Biol. Chem., 2012;287(19):15557-69. 2012 [PMID: 22393061] (Western Blot, N/A)
• Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis. Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12048-53. 2011 [PMID: 21730129] (IHC-P, Mouse)
• Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. J. Biol. Chem., 2011;286(4):2636-47. 2011 [PMID: 21075844] (Western Blot, Human)
• Nadworny PL, Wang J, Tredget EE Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond), 2010;7(0):13. 2010 [PMID: 20170497] (IHC-P, Porcine)
• Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes. Endocrinology, 2009;150(4):1688-96. 2009 [PMID: 19074584] (Western Blot, Human)
• Nitta H, Imamura T, Wada Y, Irie A, Kobayashi H, Okamoto K, Baba H Production of C5a by ASP, a serine protease released from Aeromonas sobria. J. Immunol., 2008;181(5):3602-8. 2008 [PMID: 18714034]
• Huang X, Du W, Barrett RP, Hazlett LD ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis. Invest. Ophthalmol. Vis. Sci., 2007;48(10):4626-33. 2007 [PMID: 17898286] (Western Blot, Mouse)

Review for IgG Antibody (HAF017) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human and Pig.

reviewed by: Louise Speed Simple Western Human and Pig 08/14/2019

Summary

• Application: Simple Western
• Sample Tested: human liver cell,Liver tissue,Muscle tissue,Skin tissue
• Species: Human and Pig
• Lot: WVR2718041

Bioinformatics

• Gene Symbol: IGHG1