Secondary Antibodies: Find Your Antibodies of Interest

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Secondary Antibodies

Janelia Fluor® 549 Conjugated Antibodies

Janelia Fluor® 646 Conjugated Antibodies

Antibody Labeling Kits

Antibody Resources

Protocols

Secondary Antibody Handbook

Secondary Antibody FAQs

Direct vs. Indirect Detection

Janelia Fluor® Dyes

Find your antibody of interest using the search tool below.


Browse all secondary antibodies

Choose from over 40 different labels, 26 reactive species, 10 host species, and all isotypes including fragments thereof. Our secondary antibodies are validated and optimized for use in western blot, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and more.

       Host Species        Reactivity        Target Ig Class        Conjugates

Example of Secondary Antibody Nomenclature

antibody structure
Goat:
Host Species
F(ab):
Antibody Structure
Rabbit:
Species Reactivity
(Host of the Primary
Antibody)
IgG1:
Target Immunoglobulin
(Class and subclass of
the Primary Antibody)
H+L:
Heavy + Light chain,
Target Specificity (Region
of the Primary Antibody)
HRP:
Detection Label
Pre-adsorbed:
Additional Purification
Step to Reduce Species
Cross-reactivity

Janelia Fluor® Conjugated Secondary Antibodies

janelia fluor conjugated secondary antibodies

Janelia Fluor® 549 and Janelia Fluor® 646 conjugated secondary antibodies expand the palette of bright, photostable dyes available for your dSTORM and STED imaging experiments.

Learn More


secondary antibody direct vs indirect detection

secondary antibody handbook

Direct vs. Indirect Detection

Understand how using a secondary antibody (indirect detection) can increase assay sensitivity and flexibility. Review the pros and cons of both methods to determine which is best suited for your immunoassay.

Learn more about detection methods

Secondary Antibody Handbook

This guide provides an introduction to antibody structure and nomenclature with technical information and tips for selecting the optimal secondary antibody.

Get your copy of our secondary antibody handbook