53BP1 Antibody - BSA Free

Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.

53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.

Human breast tumors stained with 53BP1 antibody. Image from verified customer review.

Detection of Human and Mouse 53BP1 by IHC. Sample: FFPE sections of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: 53BP1 Antibody (Catalog #NB100-304) used at a dilution of 1:1000 (1ug/mL). Detection: DAB.

Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent EPE. Cells were immunostained for 53BP1 (green). The nuclei were counterstained with DAPI (blue). Image from verified customer review.

An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunoprecipitation analysis lysates from HCC44 cells in 1% NP40. Image from verified customer review.

Effect of RK-33 on radiation-induced DNA damage. A Immunofluorescence images showing 53BP1 and gamma-H2AX foci in A549 cells after 2-Gy radiation and A549 cells pre-treated with 6 uM RK-33, 12 h before radiation. Overlap of 53BP1 and gamma-H2AX is seen in the merged picture of the co-immunofluorescence staining. Scale bar is 2 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.15252/emmm.201404368) licensed under a CC-BY licence.

Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS/GFP-lamin A cells were pretreated with 10 um ATMi f for 1 h before exposure to IR (10 Gy, 1 h recovery). Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analyzed by immunoblotting using 53BP1 and GFP antibodies. WCE represents 1% input. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY licence.

HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with 53BP1 Antibody conjugated to DyLight 550 (NB100-304R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

An intracellular stain was performed on Ntera2 cells with 53BP1 Antibody NB100-304 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Whole cell lysate from U2OS or 293T cells. Bands indicate an observed molecular weight of ~220 kDa and the theoretical molecular weight is 214 kDa.

Upper Panel: 53BP1 foci in proliferating MEFs. Lower Panel: 53BP1 foci in proliferating MEFs exposed to 10 Gy of IR.

Embryonic Fibroblast cells pre-extraction for 5 mins with CSK buffer. Fixed with 4% PFA and 75% Ethanol. Primary Antibody at 1:1000. Secondary Antibody at 1:1000. Image from verified customer review.

Upper Panel: Control untreated cells. Lower Panel: Cells exposed to irradiation (10 Gy) and probed for 53BP1 foci. Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with primary antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa Fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil). Image from verified customer review.

MUS81 inhibition in BRCA2-deficient cells causes accumulation of 53BP1 nuclear bodies and G1 arrest. H1299 cells carrying a DOX-inducible BRCA2 shRNA were transfected with control or MUS81 siRNAs. Representative images of cells processed 72 h later for immunostaining with 53BP1 Antibody (green) and anti-cyclin A (red) antibodies. DNA was counterstained with DAPI. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms15983), licensed under a CC-BY licence.

Pot1a prevents DDR in HSCs. Telomeric DDR in 8 week-old LSK cells upon Pot1a knockdown. Immunocytochemical staining of TRF1 (green). Foci co-stained with TRF1 and 53BP1 were identified as TIFs. Nuclei were stained with TOTO3 (blue). Scale bar, 2 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00935-4), licensed under a CC-BY licence.

Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS cells were transfected with siCTRL or siLMNA and subjected to laser micro-irradiation, fixed 15 min later and then processed for immunofluorescence with gamma-H2AX and 53BP1 antibodies. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY licence.

Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody NB100-304 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody NB100-304 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with 53PB1 Antibody conjugated to DyLight 550 (NB100-304R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Staining of 53BP1 in human colon cancer using DAB with hematoxylin counterstain.

An intracellular stain was performed on A431 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

For 53BP1 staining, samples were incubated for 1 hour with 1:200 53BP1 primary antibody in PBS/BSA 1%, at room temperature. Green: 53BP Blue: DAPI. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm, Po, Pm, Fe, Pm-Or, Rb, Sh
• Applications: WB, ChIP, Flow, Func, ICC/IF, IHC, IP, WB, ChIP, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

53BP1 Antibody - BSA Free Summary

• Description: Please note for Lot A5, the dilution recommendations have changed from our previous lot recommendations. You may be required to use the antibody at a higher dilution than previous lots to obtain similar results. Please contact us if you have any questions.
• Immunogen: Partial synthetic peptide made to an internal portion of human 53BP1 (between amino acids 350-400) [NP_005648.1].
• Marker: DNA Double Strand Break Marker
• Predicted Species: Porcine (90%), Chimpanzee (96%), Orangutan (96%), Rabbit (90%), Feline (90%), Sheep (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 24591601)
  • Flow (Intracellular)
  • Flow Cytometry 1.5 ug/ml
  • Immunoblotting reported in multiple pieces of scientific literature
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry reported in scientific literature (PMID 24987917)
  • Immunohistochemistry-Frozen reported by customer review
  • Immunohistochemistry-Paraffin 1:1000-1:5000. Use reported in scientific literature (PMID 27653664)
  • Immunoprecipitation reported in scientific literature (PMID 25645366)
  • In-situ Hybridization reported in scientific literature (PMID 34988401; 33122290)
  • Knockdown Validated
  • Knockout Validated reported in scientific literature (PMID 26601238)
  • Western Blot 1:5000-1:25000
• Theoretical MW: 214 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Rectum Whole Tissue Lysate (Adult Whole Tumor)
  Human Ovary Whole Tissue Lysate (Adult Whole Tumor)

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32877678). Human and mouse reactivity cited in numerous publications. Primate reactivity reported in scientific literature (PMID: 18389475). Fish reactivity reported in scientific literature (PMID: 25516420). Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). Predicted cross-reactivity based on sequence identity: Chimpanzee (96%), Gorilla (96%), Orangutan (96%), Gibbon (94%), Marmoset (92%), Feline (90%), Porcine (90%), Rabbit (90%), Sheep (90%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note for Lot A5, the dilution recommendations have changed from our previous lot recommendations. You may be required to use the antibody at a higher dilution than previous lots to obtain similar results. Please contact us if you have any questions.

Alternate Names for 53BP1 Antibody - BSA Free

• 53BP1
• p202
• p53-binding protein 1
• p53bp1
• TDRD30
• TP53-Binding Protein 1
• TP53BP1
• tumor protein 53-binding protein, 1
• tumor protein p53 binding protein 1
• tumor protein p53-binding protein, 1
• tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 53BP1 Antibody (NB100-304)(623)

Publications using NB100-304

• Oropeza E, Seker S, Carrel S et al. Molecular portraits of cell cycle checkpoint kinases in cancer evolution, progression, and treatment responsiveness Science Advances 2023-06-30 [PMID: 37390209]
• Chen B, Ge T, Jian M et al. Transmembrane nuclease NUMEN/ENDOD1 regulates DNA repair pathway choice at the nuclear periphery Nature cell biology 2023-06-15 [PMID: 37322289]
• Choi YJ, Kim MS, Rhoades JH et al. Patient-Induced Pluripotent Stem Cell-Derived Hepatostellate Organoids Establish a Basis for Liver Pathologies in Telomeropathies Cellular and molecular gastroenterology and hepatology 2023-06-09 [PMID: 37302654] (Human)
• Bloom SI, Liu Y, Tucker JR et al. Endothelial cell telomere dysfunction induces senescence and results in vascular and metabolic impairments Aging cell 2023-05-31 [PMID: 37259606] (IF-FISH, Mouse)
• Heckenbach I, Mkrtchyan G, Ezra M et al. Nuclear morphology is a deep learning biomarker of cellular senescence Nature Aging 2022-08-15 [PMID: 37118134] (ICC/IF, Human)
• Sadideen D, Chen B, Basili M, Shaheen M Ubiquitin-Specific-Processing Protease 47, A Novel 53BP1 regulator bioRxiv 2021-01-28 (WB)
• Göder A, Quinlan A, Rainey M et al. PTBP1 enforces ATR-CHK1 signalling determining the potency of CDC7 inhibitors iScience 2023-05-01 [PMID: 37378325] (FLOW, Human)
  
  Details:
  1:200 Flow dilution
• Zhang X, Duan J, Li Y et al. NKAP acts with HDAC3 to prevent R-loop associated genome instability Cell death and differentiation 2023-07-01 [PMID: 37322264] (KD, IP, WB, ICC/IF, Human)
• Sachsenweger J, Jansche R, Merk T et al. ABRAXAS1 orchestrates BRCA1 activities to counter genome destabilizing repair pathways-lessons from breast cancer patients Cell death & disease 2023-05-17 [PMID: 37198153] (ICC/IF)
• Barreto-Galvez A, Niljikar M, Gagliardi J et al. Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks bioRxiv : the preprint server for biology 2023-04-29 [PMID: 37163075] (Human)
  
  Details:
  Used in Mitotic DNA Synthesis (MiDAS); Dilution: 1:1000
• Harbour JW, Kurtenbach S, Sanchez M et al. PRAME induces genomic instability in uveal melanoma Research square 2023-04-26 [PMID: 37162820] (ICC/IF, Human)
• Zech HBAA Vergleich der DNA-Doppelstrangbruch-ReparaturkapazitAt HPV-positiver und negativer Oropharynxkarzinome im Ex-vivo-Assay Thesis 2020-01-01
• Liberal F, McMahon S Characterization of Intrinsic Radiation Sensitivity in a Diverse Panel of Normal, Cancerous and CRISPR-Modified Cell Lines International Journal of Molecular Sciences 2023-04-26 [PMID: 37175568] (ICC/IF, Human)
  
  Details:
  Supplementary Figure S4
• Li Z, Jiao X, Robertson AG et al. The DACH1 gene is frequently deleted in prostate cancer, restrains prostatic intraepithelial neoplasia, decreases DNA damage repair, and predicts therapy responses Oncogene 2023-04-24 [PMID: 37095257] (IHC, Mouse)
• Rosa M, Barnes RP, Nyalapatla PR et al. OGG1 and MUTYH repair activities promote telomeric 8-oxoguanine induced cellular senescence bioRxiv : the preprint server for biology 2023-04-11 [PMID: 37090589] (ICC/IF, ISH, Human)
• Bao G, Zhou H, Zou S et al. Inhibition of Poly(ADP-ribose) Polymerase Sensitizes [177Lu]Lu-DOTAGA.(SA.FAPi)2-Mediated Radiotherapy in Triple-Negative Breast Cancer Molecular pharmaceutics 2023-05-01 [PMID: 37067162]
• Huang J, Wu C, Kloeber JA et al. SLFN5-mediated chromatin dynamics sculpt higher-order DNA repair topology Molecular cell 2023-02-25 [PMID: 36854302] (WB, ICC/IF, Human)
• Sreekumar S, Zhou D, Mpoy C et al. Preclinical Efficacy of a PARP-1 Targeted Auger-Emitting Radionuclide in Prostate Cancer International journal of molecular sciences 2023-02-04 [PMID: 36834491] (ICC/IF, Human)
• Stock AJ, Ayyar S, Kashyap A et al. Boosting NAD ameliorates hematopoietic impairment linked to short telomeres in vivo GeroScience 2023-02-24 [PMID: 36826621] (ICC/IF, Mouse)
• Alagia A, Ketley RF, Gullerova M Detecting RNA-protein proximity at DNA double-strand breaks using combined fluorescence in situ hybridization with proximity ligation assay STAR protocols 2023-02-03 [PMID: 36825808]
• Su B, Lim D, Qi C et al. VPA mediates bidirectional regulation of cell cycle progression through the PPP2R2A-Chk1 signaling axis in response to HU Cell death & disease 2023-02-13 [PMID: 36781846] (IHC-P, Rat)
• Yang JH, Hayano M, Griffin PT et al. Loss of epigenetic information as a cause of mammalian aging Cell 2023-01-19 [PMID: 36638792] (ICC/IF, Mouse)
  
  Details:
  See supplemental figure S1
• Petrosius V, Benada J, Nielsen O et al. Temporal phosphoproteomics reveals WEE1-dependent control of 53BP1 pathway iScience 2023-01-01 [PMID: 36632060] (ICC/IF, Human)
• Sigismondo G, Arseni L, Palacio-Escat N et al. Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair Nucleic acids research 2023-01-25 [PMID: 36629267] (ChIP, Human)
• Schuhwerk H, Kleemann J, Gupta P et al. The EMT transcription factor ZEB1 governs a fitness-promoting but vulnerable DNA replication stress response Cell reports 2022-12-13 [PMID: 36516781]
• Kriger D, Novitskaya K, Vasileva G et al. Alpha-actnin-4 (ACTN4) selectively affects the DNA double-strand breaks repair in non-small lung carcinoma cells Biology direct 2022-12-07 [PMID: 36476259] (ICC/IF, Human)
• Schwab N, Taskina D, Leung E et al. Neurons and glial cells acquire a senescent signature after repeated mild traumatic brain injury in a sex-dependent manner Frontiers in neuroscience 2022-11-03 [PMID: 36408415]
• Madden-Hennessey K, Gupta D, Radecki AA et al. Loss of mismatch repair promotes a direct selective advantage in human stem cells Stem cell reports 2022-10-27 [PMID: 36368329] (ICC/IF, Human)
• Bloom SI, Tucker JR, Lim J et al. Aging results in DNA damage and telomere dysfunction that is greater in endothelial versus vascular smooth muscle cells and is exacerbated in atheroprone regions GeroScience 2022-11-09 [PMID: 36350415] (ISH, Mouse)
  
  Details:
  Dilution used for ISH 1:500
• Mota A, Berezicki S, Wernersson E et al. FRET-FISH probes chromatin compaction at individual genomic loci in single cells Nature communications 2022-11-05 [PMID: 36335096] (ICC/IF, Mouse)
  
  Details:
  Dilution used in ICC 1:1000
• Grange LJ, Reynolds JJ, Ullah F et al. Pathogenic variants in SLF2 and SMC5 cause segmented chromosomes and mosaic variegated hyperploidy Nature communications 2022-11-04 [PMID: 36333305] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC 1:1000
• Evans AC, Martin KA, Saxena M et al. Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner Nanomaterials (Basel, Switzerland) 2022-10-15 [PMID: 36296810] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC 1:250
• Ketley RF, Battistini F, Alagia A et al. DNA double-strand break-derived RNA drives TIRR/53BP1 complex dissociation Cell reports 2022-10-25 [PMID: 36288694]
• GarcIa-GonzAlez R, Monte-Serrano E, MorejOn-GarcIa P et al. The VRK1 chromatin kinase regulates the acetyltransferase activity of Tip60/KAT5 by sequential phosphorylations in response to DNA damage Biochimica et biophysica acta. Gene regulatory mechanisms 2022-10-21 [PMID: 36280132]
• Sanz-Ros J, Romero-GarcIa N, Mas-Bargues C et al. Small extracellular vesicles from young adipose-derived stem cells prevent frailty, improve health span, and decrease epigenetic age in old mice Science advances 2022-10-21 [PMID: 36260670] (ISH, Mouse)
• Bouffard NA, Lee K, DeLance NM et al. Novel post-acquisition image processing to attenuate red blood cell autofluorescence for quantitative image analysis Histochemistry and cell biology 2022-10-19 [PMID: 36260111]
• Liu Y, Betori RC, Pagacz J et al. Targeting telomerase reverse transcriptase with the covalent inhibitor NU-1 confers immunogenic radiation sensitization Cell chemical biology 2022-10-20 [PMID: 36206753]
• Wang Y, Ferrucci L, Seidman MM, Liu Y An optimized proximity ligation assay to detect telomere dysfunction induced foci in human and mouse cells STAR protocols 2022-09-16 [PMID: 36035793] (PLA, Human)
  
  Details:
  Dilution used in PLA 1:1000
• Catarinella G, Nicoletti C, Bracaglia A et al. SerpinE1 drives a cell-autonomous pathogenic signaling in Hutchinson-Gilford progeria syndrome Cell death & disease 2022-08-26 [PMID: 36028501] (ICC/IF, Human)
• Groelly FJ, Dagg RA, Petropoulos M et al. Mitotic DNA synthesis is caused by transcription-replication conflicts in BRCA2-deficient cells Molecular cell 2022-08-17 [PMID: 36002001]
• Shi DD, Savani MR, Levitt MM et al. De novo pyrimidine synthesis is a targetable vulnerability in IDH mutant glioma Cancer cell 2022-08-12 [PMID: 35985343] (ICC/IF, Human)
• Wong WK, Guerra Liberal FDC, McMahon SJ DNA Repair Inhibitors Potentiate Fractionated Radiotherapy More Than Single-Dose Radiotherapy in Breast Cancer Cells Cancers 2022-08-04 [PMID: 35954456] (ICC/IF, Human)
  
  Details:
  Dilution used 1:5000
• Donne R, Saroul-Ainama M, Cordier P et al. Replication stress triggered by nucleotide pool imbalance drives DNA damage and cGAS-STING pathway activation in NAFLD Developmental cell 2022-06-23 [PMID: 35768000]
• Vincendeau E, Wei W, Zhang X et al. SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination Nature communications 2022-06-28 [PMID: 35764636] (WB, Mouse)
  
  Details:
  1:1000 dilution
• Tsaridou S, Velimezi G, Willenbrock F et al. 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling EMBO reports 2022-08-03 [PMID: 35758159] (ICC/IF, WB, Human)
  
  Details:
  1:200 Dilution
• Guo Y, Chomiak AA, Hong Y et al. Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration iScience 2022-07-15 [PMID: 35754718] (WB, Mouse)
• Manandhar M, Bright SJ, Flint DB et al. Effect of boron compounds on the biological effectiveness of proton therapy Medical physics 2022-06-26 [PMID: 35754208]
• MartInez P, SAnchez-VAzquez R, Ferrara-Romeo I et al. A mouse model for Li-Fraumeni-Like Syndrome with cardiac angiosarcomas associated to POT1 mutations PLoS genetics 2022-06-01 [PMID: 35727838] (IF/IHC, ICC/IF, Mouse)
• Matsuzaki K, Kumatoriya K, Tando M et al. Polyphenols from persimmon fruit attenuate acetaldehyde-induced DNA double-strand breaks by scavenging acetaldehyde Scientific reports 2022-06-18 [PMID: 35717470]
• Borel V, Boeing S, Van Wietmarschen N et al. Disrupted control of origin activation compromises genome integrity upon destabilization of Pol epsilon and dysfunction of the TRP53-CDKN1A/P21 axis Cell reports 2022-05-31 [PMID: 35649380] (ICC/IF, Mouse)
• Tang M, Chen Z, Wang C et al. Histone chaperone ASF1 acts with RIF1 to promote DNA end-joining in BRCA1-deficient cells The Journal of biological chemistry 2022-04-23 [PMID: 35472331]
• Aminu B, Fux J, Mallette E et al. Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity Biomolecules 2022-03-22 [PMID: 35454069] (WB, Human)
• Mitrentsi I, Lou J, Kerjouan A et al. Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations Molecular cell 2022-04-13 [PMID: 35447083] (ICC/IF, Human)
• Gallant K, Bektas A, Kaileh M et al. HiIDDD: a high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells Scientific reports 2022-04-15 [PMID: 35428779] (ICC/IF, Human)
• Gunther R, Pal A, Williams C et al. Alteration of Mitochondrial Integrity as Upstream Event in the Pathophysiology of SOD1-ALS Cells [PMID: 35406813] (ICC/IF, Human)
• Fujita H, Ikeda M, Ui A et al. CHAMP1-POGZ counteracts the inhibitory effect of 53BP1 on homologous recombination and affects PARP inhibitor resistance Oncogene 2022-04-07 [PMID: 35393543] (WB, ICC/IF, Human)
• Scherthan H, Wagner SQ, GrundhOfer J et al. Planar Proton Minibeam Irradiation Elicits Spatially Confined DNA Damage in a Human Epidermis Model Cancers 2022-03-17 [PMID: 35326696] (IF/IHC, Human)
• Chardon F, Japaridze A, Witt H et al. CENP-B-mediated DNA loops regulate activity and stability of human centromeres Molecular cell 2022-03-17 [PMID: 35320753]
• Goy E, Tomezak M, Facchin C et al. The out-of-field dose in radiation therapy induces delayed tumorigenesis by senescence evasion eLife 2022-03-18 [PMID: 35302491] (ICC/IF, Mouse)
• Lee D, Apelt K, Lee SO et al. ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination Nucleic acids research 2022-03-07 [PMID: 35253893] (WB, ICC/IF, Human)
• Zhang T, Xu C, Zheng P et al. Glaucocalyxin B Attenuates Ovarian Cancer Cell Growth and Cisplatin Resistance In Vitro via Activating Oxidative Stress Oxidative Medicine and Cellular Longevity 2022-02-25 [PMID: 35251480] (ICC/IF, Human)
• Dorman LC, Nguyen PT, Escoubas CC et al. A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosis bioRxiv : the preprint server for biology 2022-02-22 [PMID: 35233577] (ICC/IF, Mouse)
• Setiaputra D, Escribano-DIaz C, Reinert JK et al. RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1 Molecular cell 2022-02-16 [PMID: 35216668]
• Harhouri K, Cau P, Casey F et al. MG132 Induces Progerin Clearance and Improves Disease Phenotypes in HGPS-like Patients' Cells Cells 2022-02-10 [PMID: 35203262] (ICC/IF, Human)
• Osborne H, Larrosa I, Schmidt C Sesquiterpene Lactones Potentiate Olaparib-Induced DNA Damage in p53 Wildtype Cancer Cells International Journal of Molecular Sciences 2022-01-20 [PMID: 35163037] (ICC/IF, Human)
• Bosso G, Lanuza-Gracia P, PiNeiro-Hermida S et al. Early differential responses elicited by BRAFV600E in adult mouse models Cell death & disease 2022-02-10 [PMID: 35145078] (IF/IHC, Mouse)
• Dutto I, Gerhards J, Herrera A et al. Pathway-specific effects of ADSL deficiency on neurodevelopment eLife 2022-02-08 [PMID: 35133277] (ICC/IF, Human)
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  Details:
  Citation using the DyLight 550 version of this antibody.
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  Details:
  A wester blot was performed using the 53BP1 antibody in WT and independent AHNAK-/- U2OS human cell lines. The interactions shown on multiple western blots determined that AHNAK restrains 53BP1-mediated p53 activation.
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  Details:
  NIH/3T3 cells and HEK292T cells (both treated with CX-5461 and untreated) underwent immunofluorescence microscopy
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• Jaiswal H, Benada J, Mullers E et al. ATM/Wip1 activities at chromatin control Plk1 re-activation to determine G2 checkpoint duration. EMBO J. 2017-07-14 [PMID: 28607002] (ICC/IF, Human)
• Garzon J, Rodriguez R, Kong Z et al. Shortage of dNTPs underlies altered replication dynamics and DNA breakage in the absence of the APC/C cofactor Cdh1. Oncogene. 2017-06-12 [PMID: 28604743] (Mouse)
• Ha K, Ma C, Lin H et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017-06-12 [PMID: 28604711] (ICC/IF, Human)
• Di Marco S, Hasanova Z et al. RECQ5 Helicase Cooperates with MUS81 Endonuclease in Processing Stalled Replication Forks at Common Fragile Sites during Mitosis. Mol Cell 2017-01-06 [PMID: 28575661] (ICC/IF, Human)
• Hilmi K, Jangal M, Marques M et al. CTCF facilitates DNA double-strand break repair by enhancing homologous recombination repair. Sci Adv 2017-05-01 [PMID: 28560323] (WB, Human)
• Contrepois K, Coudereau C, Benayoun BA et al. Histone variant H2A.J accumulates in senescent cells and promotes inflammatory gene expression Nat Commun. 2017-05-09 [PMID: 28489069] (IHC-P, Human)
• Liu G, Wang H et al. The Effect of VPA on Increasing Radiosensitivity in Osteosarcoma Cells and Primary-Culture Cells from Chemical Carcinogen-Induced Breast Cancer in Rats. Int J Mol Sci 2017-10-05 [PMID: 28489060] (ICC/IF, Human)
• Baselet Bjorn, Belmans Niels, Coninx Emma et al. Functional Gene Analysis Reveals Cell Cycle Changes and Inflammation in Endothelial Cells Irradiated with a Single X-ray Dose. Front Pharmacol 2017-01-01 [PMID: 28487652] (ICC/IF, Human)
• Farg M A, Konopka A et al. The DNA damage response (DDR) is induced by the C9orf72 repeat expansion in amyotrophic lateral sclerosis. Hum Mol Genet 2017-01-08 [PMID: 28481984] (WB, Human)
• Meloche J, Lampron M C et al. Implication of Inflammation and Epigenetic Readers in Coronary Artery Remodeling in Patients With Pulmonary Arterial Hypertension. Arterioscler Thromb Vasc Biol 2017-01-08 [PMID: 28473439] (IF/IHC, Human)
• Latella L, Dall'Agnese A, Sesillo FB et al. DNA damage signaling mediates the functional antagonism between replicative senescence and terminal muscle differentiation. Genes Dev. 2017-04-01 [PMID: 28446595]
• Huang D, Lu W, Zou S et al. Rho GDP-dissociation inhibitor alpha is a potential prognostic biomarker and controls telomere regulation in colorectal cancer Cancer Sci. 2017-07-01 [PMID: 28417530] (IHC-P, Human)
• Cottineau J, Kottemann MC, Lach FP et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J. Clin. Invest. 2017-05-01 [PMID: 28414293] (ICC/IF, Human)
• Vancevska A, Douglass KM, Pfeiffer V et al. The telomeric DNA damage response occurs in the absence of chromatin decompaction. Genes Dev 2017-03-15 [PMID: 28381410] (WB, Human)
• Dixon CR, Platani M, Makarov AA, Schirmer EC. Microinjection of Antibodies Targeting the Lamin A/C Histone-Binding Site Blocks Mitotic Entry and Reveals Separate Chromatin Interactions with HP1, CenpB and PML. Cells. 2017-03-25 [PMID: 28346356] (ICC/IF, Human)
• Yang TB, Chen Q, Deng JT et al. Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche. Nat Commun. 2017-03-17 [PMID: 28303901] (ICC/IF, Mouse)
• Ji S, Zhu L, Gao Y et al. Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening. Cell Res 2017-03-17 [PMID: 28303890] (ICC/IF, Mouse)
• Nonnekens J, Chatalic KL, Molkenboer-Kuenen JD et al. (213)Bi-Labeled Prostate-Specific Membrane Antigen-Targeting Agents Induce DNA Double-Strand Breaks in Prostate Cancer Xenografts. Cancer Biother. Radiopharm. 2017-03-01 [PMID: 28301262]
• Leung JW, Makharashvili N, Agarwal P et al. ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair Genes Dev. 2017-02-01 [PMID: 28242625] (Human)
• Rossiello F, Aguado J, Sepe S et al. DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs. Nat Commun. 2017-02-27 [PMID: 28239143] (ICC/IF, Mouse)
• Liu Y, Cussiol JR, Dibitetto D et al. TOPBP1(Dpb11) plays a conserved role in homologous recombination DNA repair through the coordinated recruitment of 53BP1(Rad9). J. Cell Biol. 2017-02-22 [PMID: 28228534]
• Tan R, Nakajima S, Wang Q et al. Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1. Mol. Cell. 2017-02-08 [PMID: 28216227]
• Cheng LC, Tan VM, Ganesan S, Drake JM. Integrating phosphoproteomics into the clinical management of prostate cancer. Clin Transl Med 2017-12-01 [PMID: 28197968] (WB, ICC/IF)
• Reynolds JJ, Bicknell LS, Carroll P et al. Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism. Nat. Genet. 2017-02-13 [PMID: 28191891]
• Viziteu E, Klein B, Basbous J et al. RECQ1 helicase is involved in replication stress survival and drug resistance in multiple myeloma. Leukemia 2017-03-10 [PMID: 28186131]
• Kalimutho M, Bain AL, Mukherjee B et al. Enhanced dependency of KRAS mutant colorectal cancer cells on RAD51-dependent homologous recombination repair identified from genetic interactions in Saccharomyces cerevisiae. Mol Oncol. 2017-02-07 [PMID: 28173629]
• Taniguchi J B, Kondo K et al. RpA1 ameliorates symptoms of mutant ataxin-1 knock-in mice and enhances DNA damage repair. Hum Mol Genet 2016-10-15 [PMID: 28173122] (WB, IF/IHC, Mouse)
• Feldman S, Wuerffel R, Achour I et al. 53BP1 Contributes to Igh Locus Chromatin Topology during Class Switch Recombination. J. Immunol. 2017-02-03 [PMID: 28159901] (Chemotaxis, Mouse)
• Liu X, Liu F, Gao S et al. A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5. Cancer Letters 2017-01-27 [PMID: 28137631] (ICC/IF, Human)
• Mytych J, Wos I, Solek P, Koziorowski M. Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes. Exp Cell Res 2017-01-15 [PMID: 28011195] (Human)
• Tumini E, Barroso S, Aguilera A. Roles of human POLD1 and POLD3 in genome stability. Sci Rep. 2016-12-15 [PMID: 27974823] (ICC/IF, Human)
• Wiedemann EM Peycheva M Pavri R. DNA Replication Origins in Immunoglobulin Switch Regions Regulate Class Switch Recombination in an R-Loop-Dependent Manner. Cell Rep. 2016-12-13 [PMID: 27974207] (Chemotaxis)
• Smirin-Yosef P, Zuckerman-Levin N, Tzur S et al. A biallelic mutation in the homologous recombination repair gene SPIDR is associated with human gonadal dysgenesis. J. Clin. Endocrinol. Metab. 2016-12-14 [PMID: 27967308] (Human)
• Luo K, Li L, Li Y et al. A phosphorylation-deubiquitination cascade regulates the BRCA2-RAD51 axis in homologous recombination. Genes Dev. 2016-12-01 [PMID: 27941124] (ICC/IF, Human)
• Jia P, Chastain M, Zou Y et al. Human MLH1 suppresses the insertion of telomeric sequences at intra-chromosomal sites in telomerase-expressing cells. Nucleic Acids Res. 2016-11-29 [PMID: 27903905] (Human)
• Hess J, Unger K, Orth M et al. Genomic amplification of Fanconi anemia complementation group A (FancA) in head and neck squamous cell carcinoma (HNSCC): Cellular mechanisms of radioresistance and clinical relevance. Cancer Lett. 2016-11-17 [PMID: 27867017] (Human)
• Hansen RK, Mund A, Poulsen SL et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat. Cell Biol. 2016-12-01 [PMID: 27820601] (Mouse)
• Li M, Yu X, Chen Q. Chemopreventive effects of ROS targeting in a murine model of BRCA1-deficient breast cancer. Cancer Res. 2016-11-04 [PMID: 27815389] (Human)
• Telomere shortening and metabolic compromise underlie dystrophic cardiomyopathy. Chang AC, Ong SG, LaGory EL et al. Proc. Natl. Acad. Sci. U.S.A. [PMID: 27799523] (FLOW, Mouse)
• Bezine E, Malaise Y, Loeuillet A et al. Cell resistance to the Cytolethal Distending Toxin involves an association of DNA repair mechanisms. Sci Rep 2016-10-24 [PMID: 27775089]
• Piechota M, Sunderland P, Wysocka A et al. Is senescence-associated beta-galactosidase a marker of neuronal senescence? Oncotarget. 2016-12-05 [PMID: 27768595] (ICC/IF, Rat)
• Toonen LJ, Schmidt I, Luijsterburg MS et al. Antisense oligonucleotide-mediated exon skipping as a strategy to reduce proteolytic cleavage of ataxin-3. Sci Rep 2016-10-12 [PMID: 27731380]
• Sobiepanek A, Milner-Krawczyk M, Bobecka-Wesolowska K, Kobiela T The effect of delphinidin on the mechanical properties of keratinocytes exposed to UVB radiation. J. Photochem. Photobiol. B, Biol. 2016-11-01 [PMID: 27716574] (Human)
• Coucoravas Christos, Dhanjal Soniya, Henriksson Sofia et al. Phosphorylation of the Cajal body protein WRAP53 beta by ATM promotes its involvement in the DNA damage response. Rna Biology 2017-06-03 [PMID: 27715493] (ICC/IF, Human)
• Przybylska Dorota, Janiszewska Dorota, Gozdzik Aleksandra et al. NOX4 downregulation leads to senescence of human vascular smooth muscle cells. Oncotarget 2016-01-01 [PMID: 27655718] (ICC/IF, Human)
• Imamura T, Fujita K, Tagawa K et al. Identification of hepta-histidine as a candidate drug for Huntington's disease by in silico-in vitro- in vivo-integrated screens of chemical libraries Sci Rep 2016-09-22 [PMID: 27653664] (IF/IHC, Mouse)
• Jakl L, Lobachevsky P, Vokalova L et al. Validation of JCountPro software for efficient assessment of ionizing radiation-induced foci in human lymphocytes. Int J Radiat Biol 2016-12-01 [PMID: 27648492] (Human)
• Lescale C, Lenden Hasse H, Blackford A et al. Specific Roles of XRCC4 Paralogs PAXX and XLF during V(D)J Recombination. Cell Rep 2016-09-13 [PMID: 27601299] (ICC/IF, Human)
• Bhatt N, Ghosh R, Roy S et al. Robust reprogramming of Ataxia-Telangiectasia patient and carrier erythroid cells to induced pluripotent stem cells. Stem Cell Res. 2016-08-12 [PMID: 27596957] (ICC/IF)
• Croco E, Marchionni S, Bocchini M et al. DNA Damage Detection by 53BP1: Relationship to Species Longevity. J. Gerontol. A Biol. Sci. Med. Sci. 2016-08-29 [PMID: 27573809]
• Nonnekens J, van Kranenburg M, Beerens CE et al. Potentiation of Peptide Receptor Radionuclide Therapy by the PARP Inhibitor Olaparib. Theranostics. 2016-08-29 [PMID: 27570553] (ICC/IF, Human)
• Henry A, Nokin Mj, Leroi N et al. New role of osteopontin in DNA repair and impact on Human glioblastoma radiosensitivity. Oncotarget 2016-08-22 [PMID: 27563812]
• Graciotti M, Fang Z, Johnsson K, Gonczy P. Chemical genetic screen identifies natural products modulating centriole number. Chembiochem. 2016-08-25 [PMID: 27558802] (Human)
• Montero JJ, Lopez de Silanes I, Grana O, Blasco MA. Telomeric RNAs are essential to maintain telomeres. Nat Commun. 2016-08-17 [PMID: 27531349] (ICC/IF)
• Schmidt JC, Zaug AJ, Cech TR et al. Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres Cell. 2016-08-10 [PMID: 27523609] (ICC/IF, Human)
• Taniguchi JB, Kondo K, Fujita K et al. RpA1 ameliorates symptoms of mutant ataxin-1 knock-in mice and enhances DNA damage repair. Hum. Mol. Genet. 2016-08-11 [PMID: 27516390] (IF/IHC, Mouse)
• Patel PL, Suram A, Mirani N et al. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc. Natl. Acad. Sci. U.S.A. 2016-08-23 [PMID: 27503890] (ICC/IF, Human)
• Chua S, Lee FY, Chiang HJ et al. The cardioprotective effect of melatonin and exendin-4 treatment in a rat model of cardiorenal syndrome. J. Pineal Res. 2016-07-28 [PMID: 27465663] (IF/IHC, ICC/IF, Rat)
• Butterworth KT, Nicol JR, Ghita M et al. Preclinical evaluation of gold-DTDTPA nanoparticles as theranostic agents in prostate cancer radiotherapy. Nanomedicine (Lond). 2016-08-01 [PMID: 27463088] (ICC/IF, Human)
• Lambrus BG, Daggubati V, Uetake Y et al. A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis J. Cell Biol. 2016-07-18 [PMID: 27432896] (ICC/IF, WB)
• Mao P, Liu J, Zhang Z et al. Homologous recombination-dependent repair of telomeric DSBs in proliferating human cells Nat Commun 2016-07-11 [PMID: 27396625] (ICC/IF)
• Fong CS, Mazo G, Das T et al. 53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis Elife 2016-07-16 [PMID: 27371829]
• Picco V, Coste I, Giraud-Panis MJ et al. ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2 Oncotarget. 2016-07-19 [PMID: 27366950] (ICC/IF)
• Parekh PR, Choudhuri R, Weyemi U et al. Evaluation of surrogate tissues as indicators of drug activity in a melanoma skin model. Cancer Med 2016-06-23 [PMID: 27339860] (IF/IHC)
• Jullien D, Vignard J, Fedor Y et al. Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells. J. Cell. Sci. 2016-05-20 [PMID: 27206857] (ICC/IF, Human)
• Stankovic-Valentin N, Drzewicka K, Konig C et al. Redox regulation of SUMO enzymes is required for ATM activity and survival in oxidative stress. EMBO J. 2016-05-12 [PMID: 27174643]
• Ho TLF, Guilbaud G, Blow JJ et al. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Reports. 2016-04-14 [PMID: 27149840] (IHC-P, Mouse)
• Kreienkamp R, Croke M, Neumann MA et al. Vitamin D receptor signaling improves Hutchinson-Gilford progeria syndrome cellular phenotypes. Oncotarget. 2016-04-27 [PMID: 27145372] (WB, Human)
• Pauty J, Cote MF, Rodrigue A et al. Investigation of the DNA damage response to SFOM-0046, a new small-molecule drug inducing DNA double-strand breaks Sci Rep 2016-03-22 [PMID: 27001483] (ICC/IF, Human)
• O'Hagan-Wong K, Nadeau S, Carrier-Leclerc A et al. Increased IL-6 secretion by aged human mesenchymal stromal cells disrupts hematopoietic stem and progenitor cells' homeostasis. Oncotarget 2016-03-22 [PMID: 26934440] (ICC/IF)
• Francia S, Cabrini M, Matti V et al. DICER, DROSHA and DNA damage-response RNAs are necessary for the secondary recruitment of DNA damage response factors J. Cell. Sci. 2016-02-16 [PMID: 26906421] (ICC/IF, Human)
• Cekan P, Hasegawa K, Pan Y et al. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. Mol Biol Cell 2016-04-15 [PMID: 26864624] (WB)
• Nagy Z, Kalousi A, Furst A et al. Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs. PLoS Genet 2016-02-01 [PMID: 26845027] (WB)
• Lescale C, Abramowski V, Bedora-Faure M et al. RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair. Nat Commun. 2016-02-02 [PMID: 26833222] (ICC/IF, Mouse)
• Martinez I, Garcia-Carpizo V, Guijarro T et al. Induction of DNA double-strand breaks and cellular senescence by human respiratory syncytial virus Virulence 2016-05-18 [PMID: 26809688] (ICC/IF, Human)
• Zhang H, Liu H, Chen Y et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016-01-05 [PMID: 26727879] (WB, ICC/IF, Human)
• Chatalic KLS, Konijnenberg M, Nonnekens J. In Vivo Stabilization of a Gastrin-Releasing Peptide Re-ceptor Antagonist Enhances PET Imaging and Radionu-clide Therapy of Prostate Cancer in Preclinical Studies. Theranostics . 2016-01-01 [PMID: 26722377] (IHC-P, Mouse)
• Obermeier K, Sachsenweger J, Friedl TW et al. Heterozygous PALB2 c.1592delT mutation channels DNA double-strand break repair into error-prone pathways in breast cancer patients. Oncogene. 2015-12-07 [PMID: 26640152] (WB, ICC/IF, Human)
• Rall M, Kraft D, Volcic M et al. Impact of Charged Particle Exposure on Homologous DNA Double-Strand Break Repair in Human Blood-Derived Cells. Front Oncol 2015-01-01 [PMID: 26618143] (ICC/IF, Human)
• Han X, Liu Z, Jo MC et al. CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv 2015-08-01 [PMID: 26601238] (ICC/IF)
• Li M, Huang R, Jiang X et al. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. PLoS ONE. 2015-11-25 [PMID: 26599493] (ICC/IF, Mouse)
• Werner E, Wang H, Doetsch PW. Role of Pro-inflammatory Cytokines in Radiation-Induced Genomic Instability in Human Bronchial Epithelial Cells. Radiat. Res. 2015-11-18 [PMID: 26579942] (ICC/IF, Human)
  
  Details:
  53BP1 antibody along with gamma-H2AX was used for the detection of DNA repair foci in irradiated Human Bronchial Epithelial Cells (HBEC3-KT) fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton™ X-100.
• Storch K, Cordes N. The impact of CDK9 on radiosensitivity, DNA damage repair and cell cycling of HNSCC cancer cells. Int. J. Oncol. 2015-11-13 [PMID: 26573875]
• Djuzenova CS, Zimmermann M, Katzer A et al. A prospective study on histone Y-H2AX and 53BP1 foci expression in rectal carcinoma patients: correlation with radiation therapy-induced outcome. BMC Cancer. 2015-11-06 [PMID: 26541290] (ICC/IF, Human)
• Markova E, Somsedikova A, Vasilyev S et al. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy. Int. J. Radiat. Biol. 2015-11-04 [PMID: 26537244] (ICC/IF, Human)
• Perez M, Munoz-Galvan S, Jimenez-Garcia MP et al. Efficacy of CDK4 inhibition against sarcomas depends on their levels of CDK4 and p16ink4 mRNA. Oncotarget 2015-12-01 [PMID: 26528855] (WB)
• Dalm SU, Nonnekens J, Doeswijk GN et al. Comparison of the Therapeutic Response to Treatment with a 177Lu-Labeled Somatostatin Receptor Agonist and Antagonist in Preclinical Models. J Nucl Med 2016-02-01 [PMID: 26514177]
• Hehlgans S, Oppermann J, Reichert S et al. The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis. Radiat Oncol 2015-09-19 [PMID: 26383618] (ICC/IF, Human)
• Lin H, Ha K, Lu G et al. Cdc14A and Cdc14B Redundantly Regulate DNA Double-Strand Break Repair. Mol Cell Biol 2015-11-01 [PMID: 26283732] (Mouse)
• Durdik M, Kosik P, Gursky J et al. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and gammaH2AX foci in human lymphocytes. Cytometry A. 2015-08-04 [PMID: 26243567] (FLOW, ICC/IF, Human)
• Jin Z, Sharma A, Hendzel M Argonaute 1 Promotes Histone H3k9 Trimethylation to Facilitate Homologous Recombination SSRN Electronic Journal 2021-07-23
• Chaudhary P, Marshall TI, Currell FJ et al. Variations in the Processing of DNA Double-Strand Breaks Along 60-MeV Therapeutic Proton Beams. Int J Radiat Oncol Biol Phys 2015-07-29 [PMID: 26452569]
• Kubben N, Brimacombe KR, Donegan M et al. A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Methods 2015-09-01 [PMID: 26341717] (ICC/IF, Human)
• Weaver AN, Cooper TS, Rodriguez M et al. DNA double strand break repair defect and sensitivity to poly ADP-ribose polymerase (PARP) inhibition in human papillomavirus 16-positive head and neck squamous cell carcinoma. Oncotarget 2015-09-29 [PMID: 26336991] (ICC/IF)
• Chojnowski A, Ong PF, Wong ES et al. Progerin reduces LAP2alpha-telomere association in Hutchinson-Gilford progeria. Elife 2015-01-01 [PMID: 26312502]
• Ortega-Martinez S. Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress. Redox Biol 2015-08-01 [PMID: 26160768] (ICC/IF)
• Petroni M, Sardina F, Heil C et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ 2016-02-01 [PMID: 26068589] (WB)
• Fukuda Takayo, Wu Wenwen, Okada Maiko et al. Class I histone deacetylase inhibitors inhibit the retention of BRCA1 and 53BP1 at the site of DNA damage. Cancer Science 2015-01-01 [PMID: 26053117] (WB, Human)
• Beyreuther E, Karsch L, Laschinsky L. Radiobiological response to ultra-short pulsed megavoltage electron beams of ultra-high pulse dose rate. Int. J. Radiat. Biol. 2015-05-13 [PMID: 25968557] (ICC/IF, Human)
  
  Details:
  53BP1 antibody was used for ICC-IF staining of cytospun cells of two human squamous cell carcinoma cell lines of different radiosensitivity -FaDu/undifferentiated HNSCC of moderate radiosensitivity and SKX/moderately well differentiated HNSCC of higher radiosensitivity than FaDu. Immunostaining was performed 24 h after irradiation and primary antibody was used at 1:3000 dilution with detection via AF488 conjugated secondary antibody.
• Ihle M Connections between stem cell features, EMT, DNA Replication and DNA damage response: mechanisms and therapeutic implication Thesis 2021-01-01 (ICC/IF, PLA, WB, Rabbit)
• Markiewicz E, Barnard S, Haines J et al. Nonlinear ionizing radiation-induced changes in eye lens cell proliferation, cyclin D1 expression and lens shape. Open Biol. 2015-04-01 [PMID: 25924630] (WB, ICC/IF, Human)
  
  Details:
  53BP1 antibody was used for WB and ICC-IF applications on human lens epithgelial cell line FHL124 exposed or not to low-dose IR up to 2.28 Gy. See full text for protocol details and the data images (Figure 2 and 5).
• Yu Q, Katlinskaya YV, Carbone CJ et al. DNA-Damage-Induced Type I Interferon Promotes Senescence and Inhibits Stem Cell Function Cell Rep 2015-04-21 [PMID: 25921537] (ICC/IF, Human)
• Boucher D, Vu T, Bain AL et al. Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice. FASEB J. 2015-04-27 [PMID: 25917330] (ICC/IF, Mouse)
  
  Details:
  Vimentin antibody used for IHC on mouse cryosections from wounds that were untreated and treated with an in vitro-engineered adipose dressing (Fig 1A and 1E).
• Zong D, Callen E, Pegoraro G et al. Ectopic expression of RNF168 and 53BP1 increases mutagenic but not physiological non-homologous end joining. Nucleic Acids Res. 2015-05-26 [PMID: 25916843] (WB, ICC/IF, Mouse)
• Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015-04-20 [PMID: 25895060] (ICC/IF, Human)
• Meir M, Galanty Y, Kashani L et al. The COP9 signalosome is vital for timely repair of DNA double-strand breaks Nucleic Acids Res. 2015-04-08 [PMID: 25855810] (WB, Human)
• Bol GM, Vesuna F, Xie M et al. Targeting DDX3 with a small molecule inhibitor for lung cancer therapy. EMBO Mol Med. 2015-03-27 [PMID: 25820276] (ICC/IF, Human)
• Lewinska A, Jarosz P, Czech J, Rzeszutek I. Capsaicin-induced genotoxic stress does not promote apoptosis in A549 human lung and DU145 prostate cancer cells Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2015-02-01 [PMID: 25813723] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used in ICC-IF staining application for the detection of 53BP1 foci on human lung A549 and DU145 prostate cancer cells which were treated or not with 5-250uM concentrations of Capsaicin for 24 hours (Fig. 5C). The immunoassay was performed on interphase nuclei and implicated - 20 minutes fixation of cells with 3.7% formaldehyde containing 0.1% Triton X-100 in PBS, blocking with 1%BSA-0.25% Triton X-100-PBS at RT for 30 minutes, incubation of cells with primary antibody at 1:200 dilution in blocking buffer for ON at 4C, detection with FITC-conjugated secondary antibody and Hoechst 33,342, analysis of data with reference to number of 53BP foci.
• Xu G, Chapman JR, Brandsma I et al. REV7 counteracts DNA double-strand break resection and affects PARP inhibition. Nature. 2015-03-23 [PMID: 25799992] (WB, Mouse)
• Augello MA, Berman-Booty LD, Carr R et al. Consequence of the tumor-associated conversion to cyclin D1b. EMBO Mol Med. 2015-03-18 [PMID: 25787974] (ICC/IF, Human)
• Papp SJ, Huber AL, Jordan SD et al. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. Elife. 2015-03-11 [PMID: 25756610] (IP, ICC/IF, WB, Human, Mouse)
• Mar FA, Debnath J, Stohr BA. Autophagy-Independent Senescence and Genome Instability Driven by Targeted Telomere Dysfunction Autophagy 2015-03-09 [PMID: 25751002] (ICC/IF, Human)
• Nakagawa Y, Sedukhina AS, Okamoto N et al. NF-kappaB signaling mediates acquired resistance after PARP inhibition. Oncotarget 2015-02-28 [PMID: 25686825]
• Boccardi V, Razdan N, Kaplunov J et al. Stn1 is critical for telomere maintenance and long-term viability of somatic human cells Aging Cell. 2015-02-14 [PMID: 25684230] (ICC/IF, Human)
• Aarts M, Bajrami I, Herrera-Abreu MT et al. Functional genetic screen identifies increased sensitivity to WEE1 inhibition in cells with defects in Fanconi Anaemia and HR pathways Mol. Cancer Ther. 2015-02-11 [PMID: 25673822] (ICC/IF, Human)
• Grabowska W, Kucharewicz K, Wnuk M et al. Curcumin induces senescence of primary human cells building the vasculature in a DNA damage and ATM-independent manner Age (Dordr). 2015-02-01 [PMID: 25649709] (ICC/IF)
  
  Details:
  53BP1 antibody used in ICC-IF application for the detection of DNA damage/DNA double-strand breaks during curcumin-induced senescence of human vascular smooth muscle cell /VSMCs and endothelial cells/Ecs. The assay involved - fixation of cells in 70 % ethanol, 1:500 dilution of primary antibody, detection of 53BP1 foci using Alexa 488 conjugated secondary antibody and DAPI (Fig 4).
• Gibbs-Seymour I, Markiewicz E, Bekker-Jensen S et al. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage Aging Cell. 2015-01-23 [PMID: 25645366] (IP, Human)
• Lewinska A, Wnuk M, Grabowska W et al. Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells Toxicol. Lett. 2015-03-18 [PMID: 25644192] (ICC/IF, Human)
• Torres G, Leheste JR, Ramos RL. Immunocytochemical localization of dna double-strand breaks in human and rat brain Neuroscience. 2015-01-27 [PMID: 25637486] (ICC/IF, Rat)
• Badie S, Carlos AR, Folio C et al. BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres. EMBO J 2015-02-03 [PMID: 25582120] (WB, ICC/IF, Mouse)
• Sykora P, Misiak M, Wang Y et al. DNA polymerase b deficiency leads to neurodegeneration and exacerbates Alzheimer disease phenotypes. Nucleic Acids Res 2014-12-30 [PMID: 25552414] (IHC-P, Mouse)
  
  Details:
  Using the DyLight 550 conjugated version of NB100-304, catalog number NB100-304R.
• Diotti R, Kalan S, Matveyenko A, Loayza D. DNA-directed Polymerase Subunits Play a Vital Role in Human Telomeric Overhang Processing. Mol. Cancer Res. 2014-12-17 [PMID: 25519149] (ICC/IF, Human)
• Mender I, Gryaznov S, Dikmen ZG et al. Induction of Telomere Dysfunction Mediated by the Telomerase Substrate Precursor 6-Thio-2'-Deoxyguanosine. Cancer Discov. 2014-12-16 [PMID: 25516420] (FISH)
• Ito H, Fujita K, Tagawa K et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. EMBO Mol Med. 2015-01-06 [PMID: 25510912] (IHC-P, Mouse)
• Herrera-Moyano Emilia, Moriel-Carretero Maria, Montelone Beth A, Aguilera A. The rem mutations in the ATP-binding groove of the Rad3/XPD helicase lead to Xeroderma pigmentosum-Cockayne syndrome-like phenotypes. PLoS Genet. 2014-12-11 [PMID: 25500814] (ICC/IF)
• Baranski OA, Kalinichenko VV, Adami GR. Increased FOXM1 expression can stimulate DNA repair in normal hepatocytes in vivo but also increases nuclear foci associated with senescence. Cell Prolif. 2014-12-05 [PMID: 25477198] (IHC-Fr, Mouse)
  
  Details:
  53BP1 antibody used at 1-1000 dilution for IHC-Fr application on 2% PFA fixed liver sections of C57BL/6 and Tg(TTR-FOXM1)1Rhc mice that were exposed to Diethylnitrosamine/DEN carcinogen (Figure 3 and 4).
• Uringa EJ, Baldeyron C, Odijk H et al. A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity. DNA Repair (Amst) 2015-01-01 [PMID: 25463395]
• Baltanas FC, Valero J, Alonso JR et al. Nuclear Signs of Pre-neurodegeneration. Methods Mol. Biol. 2014-11-28 [PMID: 25431056] (ICC/IF, Mouse)
• Neumayer G, Nguyen Md. TPX2 Impacts Acetylation of Histone H4 at Lysine 16: Implications for DnA Damage Response PLoS OnE et al. 2014-11-04 [PMID: 25365214] (WB, ICC/IF, Human)
  
  Details:
  TPX2 antibody used for WB and CoIP in experiments involving MCF7 as well as HeLa cells transfected or not with siRnA-TPX2.
• Zimmermann M, Kibe T, Kabir S, De Lange T. TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. Genes Dev. 2014-10-24 [PMID: 25344324] (WB, ICC/IF, Mouse)
• Fumagalli M, Rossiello F, Mondello C, D'Adda Di Fagagna F. Stable Cellular Senescence Is Associated with Persistent DDR Activation. PLoS OnE. 2014-10-24 [PMID: 25340529] (ICC/IF, Human)
• Li Z, Wang H, Wang Y et al. Effect of Radiation Quality on Mutagenic Joining of Enzymatically-Induced DnA Double-Strand Breaks in Previously Irradiated Human Cells. Radiat. Res. 2014-10-20 [PMID: 25329962]
• Gazzaniga FS, Blackburn EH An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity Blood. 2014-12-10 [PMID: 25320237] (KD, ICC/IF, Human)
• Burdak-Rothkamm S, Rothkamm K, Mcclelland K et al. BRCA1, FAnCD2 and Chk1 are potential molecular targets for the modulation of a radiation-induced DnA damage response in bystander cells. Cancer Lett. 2014-10-07 [PMID: 25304378] (ICC/IF, Human)
• Tang M, Li Y, Zhang X et al. Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) promotes non-homologous end joining and inhibits homologous recombination repair upon DnA damage. J. Biol. Chem. 2014-10-07 [PMID: 25294876] (ICC/IF, WB, Human)
• Werner E, Wang H, Doetsch PW. Opposite Roles for p38MAPK-Driven Responses and Reactive Oxygen Species in the Persistence and Resolution of Radiation-Induced Genomic Instability. PLoS ONE. 2014-10-02 [PMID: 25271419] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF of radiation exposed HBEC-3KT cells - fixed in 4% paraformaldehyde, permeabilized with PBS-0.2% TritonX-100 (Figure 2).
• Roukos V, Burgess RC, Misteli T. Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc. 2014-10-01 [PMID: 25255091]
• Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014-09-23 [PMID: 25245017] (IHC-P, Mouse)
• Taggart LE, McMahon SJ, Currell FJ et al. The role of mitochondrial function in gold nanoparticle mediated radiosensitisation. Cancer Nanotechnol. 2014-09-22 [PMID: 25243023] (ICC/IF, Human)
• Pefani DE, Latusek R, Pires I et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat. Cell Biol. 2014-09-14 [PMID: 25218637]
• Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4. Terasawa M, Shinohara A, Shinohara M. PLoS Genet. [PMID: 25166505] (FLOW, ICC/IF, Human)
• Santos J, Gil J. TRIM28/KAP1 regulates senescence. Immunol. Lett. 2014-08-23 [PMID: 25160591] (WB, ICC/IF, Human)
• Gole B, Baumann C, Mian E et al. Endonuclease G initiates DNA rearrangements at the MLL breakpoint cluster upon replication stress. Oncogene. 2014-08-18 [PMID: 25132265]
• Vassilopoulos A, Tominaga Y, Kim HS et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2014-08-04 [PMID: 25088202] (IHC-P, Mouse)
• Frescas David, de Lange Titia. Binding of TPP1 protein to TIN2 protein is required for POT1a,b protein-mediated telomere protection. J Biol Chem. 2014-08-29 [PMID: 25056954] (Mouse)
• Carlessi L, Poli EF, Bechi G et al. Functional and molecular defects of hiPSC-derived neurons from patients with ATM deficiency. Cell Death Dis 2014-07-18 [PMID: 25032865] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF on neurons derived from hiPSC /human-induced pluripotent stem cells generated from reprogrammed fibroblasts from ATM-null A-T patients and normal controls to pluripotency. Cells washed with 0.9% NaCl, air dried and fixed in 3%PFA, permeabilized in 0.5% Triton X100/PBS -5 min/4C and blocked with 5% BSA before primary labelling for staininng the nuclear foci. Figure 6e shows the IR-induced nuclear foci/53BP1 immunostaining.
• Chene G, Ouellet V, Rahimi K et al. DNA Damage Signaling and Apoptosis in Preinvasive Tubal Lesions of Ovarian Carcinoma. Int. J. Gynecol. Cancer 2014-07-01 [PMID: 24987917] (IHC-P, Human)
  
  Details:
  53BP1 antibody used at 1:10,000 dilution for IHC-P on a TMA containing benign fallopian tubes, serous tubal intraepithelial carcinoma (STIC), high-grade serous ovarian cancer (HGSC) from patients with STICs (associated ovarian cancer /AOC) from the same patient and HGSCs without STICs/non-AOC (FIGURE 1).
• Peng M, Xie J, Ucher A et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J. 2014-06-25 [PMID: 24966277] (WB, ICC/IF, Mouse, Human)
  
  Details:
  Figure 1G - WB of 53BP1 or FANCJ in U2OS cells stably expressing shRNA vectors to either control or 53BP1 (a or b) that were also transfected with siRNAs to Con or FANCJ. Figure 7 E immunofluorescence of cells with 53BP1 foci in untreated MEFs
• Naipal KA, Verkaik NS, Ameziane N et al. Functional ex vivo assay to select Homologous Recombination deficient breast tumors for PARP inhibitor treatment. Clin. Cancer Res. 2014-06-24 [PMID: 24963051] (IHC-P, Human)
  
  Details:
  For evaluating formation of ionizing radiation induced foci (IRIF), breast tumor tissue obtained from patients undergoing wide local excision or amputation for breast cancer were processed for IHC-P using anti-53BP1 (NB100-304) at 1/500 dilution. Figure S3 shows gamma-H2AX and 53BP1 nuclear foci indicate the induction of DSBs in tissue slices.
• Broering TJ, Alavattam KG, Sadreyev RI et al. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J. Cell Biol. 2014-06-09 [PMID: 24914237] (ICC/IF, Mouse)
  
  Details:
  Immunostaining of meiotic chromosome spreads (Figure 1D) and mid-pachytene spermatocytes with 53BP1 signal on XY chromatin (Figure 1E)
• Tubbs AT, Dorsett Y, Chan E et al. kAP-1 Promotes Resection of Broken DNA Ends Not Protected by Gamma-H2AX and 53BP1 in G1-Phase Lymphocytes. Mol. Cell. Biol. 2014-05-19 [PMID: 24842905] (ICC/IF, Mouse)
• Polato F, Callen E, Wong N et al. CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J. Exp. Med. 2014-05-19 [PMID: 24842372] (WB, ICC/IF, Mouse)
• Coker H, Brockdorff N. SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks. J. Cell. Sci. 2014-05-01 [PMID: 24790221] (ICC/IF, Mouse)
• Bazzi H, Anderson KV. Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc. Natl. Acad. Sci. U.S.A. 2014-04-15 [PMID: 24706806] (IHC-Fr, Mouse)
• Popuri V, Hsu J, Khadka P et al. Human RECQL1 participates in telomere maintenance. Nucleic Acids Res. 2014-03-12 [PMID: 24623817] (ICC/IF, Human)
  
  Details:
  U2OS cells, Figs 1A-D, S1E. The specificity of the TRF2 was validated with TRF2 siRNA in U2OS cells by IP/WB (Figs 1C, S1E). The TRF2 mAb co-IPapprox. d RECQL1, likewise a RECQL1 antibody co-IPapprox. d TRF2 which was recognized by the TRF2 mAb in WB.
• Leung JW, Agarwal P, Canny MD et al. Nucleosome Acidic Patch Promotes RNF168- and RING1B/BMI1-Dependent H2AX and H2A Ubiquitination and DNA Damage Signaling. PLoS Genet. 2014-03-01 [PMID: 24603765] (ICC/IF, Human)
• Zahn A, Eranki AK, Patenaude AM et al. Activation induced deaminase C-terminal domain links DNA breaks to end protection and repair during class switch recombination. Proc. Natl. Acad. Sci. U.S.A. 2014-03-03 [PMID: 24591601] (Chemotaxis, Mouse)
• Porro A, Feuerhahn S et al. TERRA-reinforced association of LSD1 with MRE11 promotes processing of uncapped telomeres. Cell Rep 2014-02-27 [PMID: 24529708] (ICC/IF, Human)
• Minneker V Insights into the formation of oncogenic chromosome rearrangements Thesis 2022-01-01 (WB)
• Lamkowski Andreas, Forcheron Fabien, Agay Diane et al. DNA damage focus analysis in blood samples of minipigs reveals acute partial body irradiation. PLoS One. 2014-02-03 [PMID: 24498326]
• Frescas D, de Lange T. et al. TRF2-tethered TIN2 can mediate telomere protection by TPP1/POT1. Mol. Cell. Biol. 2014-01-28 [PMID: 24469404] (ICC/IF, Mouse)
• Frescas D, de Lange T. et al. A TIN2 dyskeratosis congenita mutation causes telomerase-independent telomere shortening in mice. Genes Dev. 2014-01-22 [PMID: 24449270] (ICC/IF, Mouse)
• Chen B, Gilbert LA, Cimini BA et al. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 2013-12-19 [PMID: 24360272] (Human)
• Mariotti LG, Pirovano G, Savage KI et al. Use of the gamma-H2AX Assay to Investigate DNA Repair Dynamics Following Multiple Radiation Exposures. PLoS One 2013-11-29 [PMID: 24312182] (ICC/IF, Human)
• Técher H, Gopaul D, Heuzé J et al. Regulation of oncogene-induced senescence by the MRE11 and TREX1 nucleases bioRxiv 2023-03-31 (ICC/IF)
• Bielak-Zmijewska A, Wnuk M, Przybylska D et al. A comparison of replicative senescence and doxorubicin-induced premature senescence of vascular smooth muscle cells isolated from human aorta. Biogerontology. 2013-11-16 [PMID: 24243065] (ICC/IF, Human)
• Mouron S, Rodriguez-Acebes S, Martinez-Jimenez MI et al. Repriming of DNA synthesis at stalled replication forks by human PrimPol. Nat Struct Mol Biol. 2013-12-01 [PMID: 24240614] (ICC/IF, Human)
• Chan EA, Teng G, Corbett E et al. Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2. Proc Natl Acad Sci U S A. 2013-11-26 [PMID: 24218622] (ICC/IF, Mouse)
• Scelfo A, Angrisani A, Barnes B et al. Specialized Replication Mechanisms Maintain Genome Stability at Human Centromeres papers.ssrn.com 2023-03-24
• Bekes M, Okamoto K, Crist SB et al. DUB-Resistant Ubiquitin to Survey Ubiquitination Switches in Mammalian Cells. Cell Rep. 2013-11-14 [PMID: 24210823] (ICC/IF, Mouse)
• Nakashima M, Nandakumar J, Sullivan KD et al. Inhibition of Telomerase Recruitment and Cancer Cell Death. J Biol Chem 2013-11-15 [PMID: 24097987] (ICC/IF, Human)
• Croke M, Neumann MA, Grotsky DA et al. Differences in 53BP1 and BRCA1 regulation between cycling and non-cycling cells. Cell Cycle 2013-12-01 [PMID: 24091731] (ICC/IF, Human)
• Kok J Astrocyte-induced DNA damage as a mechanism of motor neuron death in amyotrophic lateral sclerosis Thesis 2022-01-01
• Caganova M, Carrisi C, Varano G et al. Germinal center dysregulation by histone methyltransferase EZH2 promotes lymphomagenesis. J Clin Invest. 2013-12-02 [PMID: 24200695] (ICC/IF, Mouse)
• Chitnis MM, Lodhia KA, Aleksic T et al. IGF-1R inhibition enhances radiosensitivity and delays double-strand break repair by both non-homologous end-joining and homologous recombination. Oncogene. 2013-11-04 [PMID: 24186206] (ICC/IF, Human)
• Oommen D, Prise KM. Down-regulation of PERK enhances resistance to ionizing radiation. Biochem Biophys Res Commun. 2013-11-08 [PMID: 24103755] (ICC/IF, Human)
• Paul M, Aaron J, Wait E et al. Distinct mobility patterns of BRCA2 molecules at DNA damage sites bioRxiv 2023-02-21 (ICC/IF, Mouse)
• Ashok Y, Mennerich D, Vela-Rodr�guez C et al. DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levels bioRxiv 2023-01-31 (WB, Human)
• Shee C, Cox BD, Gu F et al. Engineered proteins detect spontaneous DNA breakage in Human and bacterial cells. Elife. 2013-10-29 [PMID: 24171103] (ICC/IF, Human)
• Cortizas EM, Zahn A, Hajjar ME et al. Alternative End-Joining and Classical Nonhomologous End-Joining Pathways Repair Different Types of Double-Strand Breaks during Class-Switch Recombination. J Immunol. 2013-12-01 [PMID: 24146042] (WB, Mouse)
• Despars G, Carbonneau CL, Bardeau P et al. Loss of the Osteogenic Differentiation Potential during Senescence Is Limited to Bone Progenitor Cells and Is Dependent on p53. PLoS One. 2013-08-29 [PMID: 24009740] (ICC/IF, Mouse)
• Surface L. Functional analysis of the histone variant H2A.Z during lineage commitment. PLoS Genet 2013-08-31 [PMID: 23990805]
• Scheers I, Lombard C, Paganelli M et al. Human Umbilical Cord Matrix Stem Cells Maintain Multilineage Differentiation Abilities and Do Not Transform during Long-Term Culture. PLoS One. 2013-08-09 [PMID: 23951150] (ICC/IF, Human)
• Kocher S, Spies-Naumann A, Kriegs M. ATM is required for the repair of Topotecan-induced replication-associated double-strand breaks. Radiother Oncol. 2013-08-05 [PMID: 23928469] (ICC/IF, Human)
• Reduction of Nipbl impairs cohesin loading locally and affects transcription but not cohesion-dependent functions in a mouse model of Cornelia de Lange Syndrome. Remeseiro S, Cuadrado A, Kawauchi S et al. Biochim Biophys Acta. [PMID: 23920377] (ICC/IF, Mouse)
• Britton S, Coates J, Jackson SP. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol. 2013-08-05 [PMID: 23897892] (ICC/IF, Human)
• Han X, Liu D, Zhang Y et al. Akt regulates TPP1 homodimerization and telomere protection. Aging Cell 2013-07-17 [PMID: 23862686] (ICC/IF, Human)
• Feng X, Luo Z, Jiang S et al. The telomere-associated homeobox-containing protein TAH1 participates in telomere maintenance in ALT Cells. J Cell Sci 2013-06-26 [PMID: 23813958] (ICC/IF, Human)
• Gascoigne KE, Cheeseman IM. Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis. Chromosome Res 2013-07-01 [PMID: 23793898] (ICC/IF, Mouse)
• Martinez P, Gomez-Lopez G, Garcia F et al. RAP1 Protects from Obesity through Its Extratelomeric Role Regulating Gene Expression. Cell Rep 2013-06-27 [PMID: 23791526] (IHC-P, ICC/IF, Mouse)
• Yeung F, Ramirez CM, Mateos-Gomez PA et al. Nontelomeric Role for Rap1 in Regulating Metabolism and Protecting against Obesity. Cell Rep 2013-06-27 [PMID: 23791522] (ICC/IF, Mouse)
• Wei D, Parsels LA, Karnak D et al. Inhibition of Protein Phosphatase 2A Radiosensitizes Pancreatic Cancers by Modulating CDC25C/CDK1 and Homologous Recombination Repair. Clin Cancer Res 2013-08-15 [PMID: 23780887] (ICC/IF, Human)
• Elsen S, Collin-Faure V, Gidrol X, Lemercier C. The opportunistic pathogen Pseudomonas aeruginosa activates the DNA double-strand break signaling and repair pathway in infected cells. Cell Mol Life Sci 2013-06-13 [PMID: 23760206] (WB, ICC/IF, Human)
• Streppel MM, Pai S, Campbell NR et al. microRNA 223 Is Upregulated in the Multistep Progression of Barrett's Esophagus and Modulates Sensitivity to Chemotherapy by Targeting PARP1. Clin Cancer Res. 2013-08-01 [PMID: 23757351] (ICC/IF, Human)
• Kalan S, Matveyenko A, Loayza D. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet 2013-05-28 [PMID: 23755068] (ICC/IF, Human)
• Floyd SR, Pacold ME, Huang Q et al. The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. Nature 2013-06-13 [PMID: 23728299] (WB, ICC/IF, Human)
• Zhou ZW, Tapias A, Bruhn C et al. DNA damage response in microcephaly development of MCPH1 Mouse model. DNA Repair (Amst) 2013-05-14 [PMID: 23683352] (IF/IHC, ICC/IF, Mouse)
• Djuzenova CS, Elsner I, Katzer A et al. Radiosensitivity in breast cancer assessed by the histone gamma-H2AX and 53BP1 foci. Radiat Oncol 2013-04-24 [PMID: 23617930] (ICC/IF, Human)
• Taylor BJ, Nik-Zainal S, Wu YL et al. DNA deaminases induce break-associated mutation showers with implication of APOBEC3B and 3A in breast cancer kataegis. Elife 2013-04-16 [PMID: 23599896] (ICC/IF, Human)
• Ausborn NL, Wang T, Wentz SC et al. 53BP1 expression is a modifier of the prognostic value of lymph node ratio and CA 19--9 in pancreatic adenocarcinoma BMC Cancer 2013-03-26 [PMID: 23530749] (IHC-P, Human)
• Suberbielle E, Sanchez PE, Kravitz AV et al. Physiologic brain activity causes DNA double-strand breaks in neurons, with exacerbation by amyloid-beta Nat Neurosci 2013-03-24 [PMID: 23525040] (ICC/IF, Mouse)
• Zhang Y, Chen LY, Han X et al. Phosphorylation of TPP1 regulates cell cycle-dependent telomerase recruitment Proc Natl Acad Sci U S A 2013-04-02 [PMID: 23509301] (ICC/IF, Human)
• Ramsay AJ, Quesada V, Foronda M et al. POT1 mutations cause telomere dysfunction in chronic lymphocytic leukemia Nat Genet 2013-03-17 [PMID: 23502782] (ICC/IF, Human)
• Grosso S, Doyen J, Parks SK et al. MiR-210 promotes a hypoxic phenotype and increases radioresistance in human lung cancer cell lines Cell Death Dis 2013-03-14 [PMID: 23492775] (ICC/IF, Human)
• Korwek Z, Bielak-Zmijewska A, Mosieniak G et al. DNA damage-independent apoptosis induced by curcumin in normal resting human T cells and leukaemic Jurkat cells. Mutagenesis 2013-03-13 [PMID: 23486648] (ICC/IF, Human)
• Jeyapalan JC, Sedivy JM. How to measure RNA expression in rare senescent cells expressing any specific protein such as p16Ink4a. Aging (Albany NY) 2013-02-01 [PMID: 23454889] (ICC/IF, Human)
• Dreesen O, Chojnowski A, Ong PF et al. Lamin B1 fluctuations have differential effects on cellular proliferation and senescence. J Cell Biol 2013-03-04 [PMID: 23439683] (ICC/IF, Human)
• Bohringer M, Obermeier K, Griner N et al. siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors. Oncogene 2013-02-25 [PMID: 23435420] (ICC/IF, Mouse)
• Berti M, Chaudhuri AR, Thangavel S et al. Human RECQ1 promotes restart of replication forks reversed by DNA topoisomerase I inhibition. Nat Struct Mol Biol 2013-02-10 [PMID: 23396353] (ICC/IF, Human)
• Okamoto K, Bartocci C, Ouzounov I et al. A two-step mechanism for TRF2-mediated chromosome-end protection. Nature 2013-02-06 [PMID: 23389450] (ICC/IF, Mouse)
• Grotsky DA, Gonzalez-Suarez I, Novell A et al. BRCA1 loss activates cathepsin L-mediated degradation of 53BP1 in breast cancer cells. J Cell Biol 2013-01-21 [PMID: 23337117] (ICC/IF, IHC-P, WB, Human)
• Zimmermann M, Lottersberger F, Buonomo SB et al. 53BP1 regulates DSB repair using Rif1 to control 5' end resection. Science 2013-02-08 [PMID: 23306437] (Mouse)
• Stout GJ, Blasco MA. Telomere length and telomerase activity impact the UV sensitivity syndrome xeroderma pigmentosum Cancer Res 2013-01-03 [PMID: 23288511] (Mouse)
• Sotiropoulou PA, Karambelas AE, Debaugnies M et al. BRCA1 deficiency in skin epidermis leads to selective loss of hair follicle stem cells and their progeny Genes Dev 2012-12-27 [PMID: 23271346] (ICC/IF, IF/IHC, Mouse)
• Caretti V, Hiddingh L, Lagerweij T et al. WEE1 Kinase Inhibition Enhances the Radiation Response of Diffuse Intrinsic Pontine Gliomas. Mol Cancer Ther 2013-02-01 [PMID: 23270927] (ICC/IF, Human)
• Courilleau C, Chailleux C, Jauneau A et al. The chromatin remodeler p400 ATPase facilitates Rad51-mediated repair of DNA double-strand breaks J Cell Biol 2012-12-24 [PMID: 23266955] (ICC/IF, Human)
• Pei H, Wu X, Liu T et al. The Histone Methyltransferase MMSET Regulates Class Switch Recombination J Immunol 2012-12-14 [PMID: 23241889] (WB, Mouse)
• Hartlerode AJ, Guan Y, Rajendran A et al. Impact of Histone H4 Lysine 20 Methylation on 53BP1 Responses to Chromosomal Double Strand Breaks PLoS One 2012-01-01 [PMID: 23209566] (ICC/IF, Mouse)
• Ziebarth AJ, Nowsheen S, Steg AD et al. Endoglin (CD105) contributes to platinum resistance and is a target for tumor-specific therapy in epithelial ovarian cancer. Clin Cancer Res 2013-01-01 [PMID: 23147994] (ICC/IF, IF/IHC, Mouse)
• Rodrigue A, Coulombe Y, Jacquet K et al. The RAD51 paralogs ensure cellular protection against mitotic defects and aneuploidy. J Cell Sci. 2013-01-01 [PMID: 23108668] (ICC/IF, Human)
• Popuri V, Croteau DL, Brosh RM Jr, Bohr VA. RECQ1 is required for cellular resistance to replication stress and catalyzes strand exchange on stalled replication fork structures Cell Cycle 2012-11-15 [PMID: 23095637] (ICC/IF, Human)
• Mirza S, Katafiasz BJ, Kumar R et al. Alteration/deficiency in activation-3 (Ada3) plays a critical role in maintaining genomic stability Cell Cycle 2012-11-15 [PMID: 23095635] (WB, Mouse)
• Turner R. Relationships between nuclear lamins and telomere biology in progeroid laminopathies and cancer. Thesis. 2015-01-01 (WB, ICC/IF, Human)
• Mokrani-Benhelli H, Gaillard L, Biasutto P et al. Primary Microcephaly, Impaired DNA Replication, and Genomic Instability Caused by Compound Heterozygous ATR Mutations Hum Mutat 2012-10-30 [PMID: 23111928] (ICC/IF, Human)
• Nandakumar J, Bell CF, Weidenfeld I et al. The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity. Nature. 2012-12-13 [PMID: 23103865] (ICC/IF, Human)
• Korwek Z, Sewastianik T, Bielak-Zmijewska A et al Inhibition of ATM blocks the EPE-induced DNA damage response and apoptosis of resting human T cells DNA Repair (Amst). 2012-11-01 [PMID: 23058634] (ICC/IF, Human)
• Mund A, Schubert T, Staege H et al. SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction and DNA damage response Nucleic Acids Res 2012-10-02 [PMID: 23034801] (ICC/IF, Human)
• Popova T, Manie E, Rieunier G et al. Ploidy and Large-Scale Genomic Instability Consistently Identify Basal-like Breast Carcinomas with BRCA1/2 Inactivation Cancer Res 2012-10-22 [PMID: 22933060] (ICC/IF, Human)
• Squatrito M, Vanoli F, Schultz N et al. 53BP1 Is a Haploinsufficient Tumor Suppressor and Protects Cells from Radiation Response in Glioma Cancer Res 2012-10-15 [PMID: 22915756] (IF/IHC, Mouse)
• Eke I, Storch K, Kastner I et al. Three-dimensional Invasion of Human Glioblastoma Cells Remains Unchanged by X-ray and Carbon Ion Irradiation In Vitro Int J Radiat Oncol Biol Phys 2012-08-14 [PMID: 22901381] (ICC/IF, Human)
• Eke I, Dickreuter E, Cordes N. Enhanced radiosensitivity of head and neck squamous cell carcinoma cells by ?1 integrin inhibition Radiother Oncol 2012-06-28 [PMID: 22748391] (ICC/IF, Human)
• Feng W, Jasin M. 53BP1 nuclear body-marked replication stress in a human mammary cell model of BRCA2 deficiency. J. Agric. Food Chem. 2018-11-27 (ICC/IF, Human)
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• Gonzalez-Suarez I, Redwood AB, Grotsky DA et al. A new pathway that regulates 53BP1 stability implicates cathepsin L and vitamin D in DNA repair. EMBO J 2011-07-12 [PMID: 21750527] (ICC/IF, WB, Mouse)
• Redwood AB, Perkins SM, Vanderwaal RP et al. A dual role for A-type lamins in DNA double-strand break repair. Cell Cycle 2011-08-01 [PMID: 21701264] (ICC/IF)
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• Vancevska A, Pfeiffer V, Feretzaki M, Ahmed W SMCHD1 Promotes ATM-dependent DNA Damage Signaling and Repair of Uncapped Telomeres bioRxiv (ICC/IF, Human)
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• Chayot R, Danckaert A, Montagne B, Ricchetti M. Lack of DNA polymerase mu affects the kinetics of DNA double-strand break repair and impacts on cellular senescence. DNA Repair (Amst) 2010-11-10 [PMID: 20947452] (Mouse)
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• Bouwman P, Aly A, Escandell JM et al. 53BP1 loss rescues BRCA1 deficiency and is associated with triple-negative and BRCA-mutated breast cancers. Nat Struct Mol Biol 2010-06-01 [PMID: 20453858] (WB)
• Kibe T, Osawa GA, Keegan CE et al. Telomere protection by TPP1 is mediated by POT1a and POT1b. Mol Cell Biol. 2009-12-07 [PMID: 19995905]
• Kim GJ, Georg I, Scherthan H et al. Dicer is required for Sertoli cell function and survival. Int J Dev Biol 2010-01-01 [PMID: 19876815] (IF/IHC, Mouse)
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Reviews for 53BP1 Antibody (NB100-304) (15)

Average Rating: 4.7

(Based on 15 reviews)

We have 15 reviews tested in 3 species: Human, Mouse, Human and Mouse.

reviewed by: Patricia Carulla Martí ICC Human 06/19/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured Human Keratinocytes
• Species: Human
• Lot: GR3310237-9

reviewed by: Takahiro Ito ICC Human 05/24/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: IMR-90 human lung fibroblast cell line
• Species: Human
• Lot: D-6

reviewed by: Ingrid MONTARRAS ICC Human 08/08/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Cancer Cells
• Species: Human
• Lot: E-4

Comments

• Comments: 1/1000 dilution

reviewed by: Pauline Jeannot IP Human 02/19/2019

Summary

• Application: Immunoprecipitation
• Sample Tested: human lung cancer cell line
• Species: Human
• Lot: E-6

reviewed by: Wade Baker ICC Mouse 02/04/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A

reviewed by: Sammy Ferri Borgogno WB Mouse 11/27/2018

Summary

• Application: Western Blot
• Sample Tested: mouse epithelial pancreatic cancer cells
• Species: Mouse
• Lot: D-9 and D-10

Comments

• Comments: Membrane was blocked with 5% non-fat dry milk, 1h RT. Then incubated ON 4C with 53bp1 antibody 1:10000. After washes, membrane was incubated with HRP-conjugated secondary antibody diluted in 5% non-fat dry milk.

reviewed by: Verified Customer ICC Human and Mouse 08/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured cell lines
• Species: Human and Mouse
• Lot: D-6

reviewed by: Darshil Patel IF Mouse 07/18/2017

Summary

• Application: Immunofluorescence
• Sample Tested: MEF
• Species: Mouse

reviewed by: NIKHIL VAD ICC Human 06/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: A172 Glioblastoma cells
• Species: Human
• Lot: D-2-092616-AF488

Comments

• Comments: Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with 53BP1 (AF488 conjugated) antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil).

reviewed by: Grant Stewart ICC Human 05/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts,hela cell
• Species: Human

reviewed by: Youwei Zhang ICC Human 01/04/2016

Summary

• Application: Immunocytochemistry
• Species: Human
• Lot: multiple lots

reviewed by: cynthia morimoto WB Human 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23755068
• Species: Human

reviewed by: Verified Customer IHC-Fr Mouse 01/21/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse cochlear spiral ganglion cells
• Species: Mouse
• Lot: 3

reviewed by: Elizabeth IHC Mouse 07/14/2010

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse thymocytes
• Species: Mouse

reviewed by: System Admin ICC Mouse 05/27/2009

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A

FAQs for 53BP1 Antibody (NB100-304). (Showing 1 - 4 of 4 FAQs).

1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
   • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
   • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.
3. What is the recommended pH of sodium citrate for the 53bp1 antibody (NB100-304)?
   • I have checked our notes for use of this antibody in IHC, and they say: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
4. Is possible to know the exact sequence of the epitope used?
   • Unfortunately I am unable to disclose the sequence of this antibody due to proprietary restrictions. At the site recognized by NB100-304, the reference sequences are too divergent to expect NB100-304 to bind to either Bovine or Little Brown Bat. Regarding Mouse Homology, 30/34 sites of the immunogen are homologous Human - 100% homologous. If you would be interested in testing these novel species, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: TP53BP1