53BP1 Antibody

Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.

Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Human breast tumors stained with 53BP1 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Detection of Human and Mouse 53BP1 by IHC. Sample: FFPE sections of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: 53BP1 Antibody (Catalog #NB100-304) used at a dilution of 1:1000 (1ug/mL). Detection: DAB.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent EPE. Cells were immunostained for 53BP1 (green). The nuclei were counterstained with DAPI (blue). Image from verified customer review.

Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunoprecipitation: 53BP1 Antibody [NB100-304] - Immunoprecipitation analysis lysates from HCC44 cells in 1% NP40. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Effect of RK-33 on radiation-induced DNA damage. A Immunofluorescence images showing 53BP1 and gamma-H2AX foci in A549 cells after 2-Gy radiation and A549 cells pre-treated with 6 uM RK-33, 12 h before radiation. Overlap of 53BP1 and gamma-H2AX is seen in the merged picture of the co-immunofluorescence staining. Scale bar is 2 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.15252/emmm.201404368) licensed under a CC-BY licence.

Western Blot: 53BP1 Antibody [NB100-304] - Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS/GFP-lamin A ceels were pretreated with 10 um ATMi f for 1 h before exposure to IR (10 Gy, 1 h recovery). Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analyzed by immunoblotting using 53BP1 and GFP antibodies. WCE represents 1% input. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS cells were transfected with siCTRL or siLMNA and subjected to laser micro-irradiation, fixed 15 min later and then processed for immunofluorescence with gamma-H2AX and 53BP1 antibodies. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY licence.

Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on A431 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: 53BP1 Antibody [NB100-304] - Whole cell lysate from U2OS or 293T cells. Bands indicate an observed molecular weight of ~220 kDa and the theoretical molecular weight is 214 kDa.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: 53BP1 foci in proliferating MEFs. Lower Panel: 53BP1 foci in proliferating MEFs exposed to 10 Gy of IR.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Embryonic Fibroblast cells pre-extraction for 5 mins with CSK buffer. Fixed with 4% PFA and 75% Ethanol. Primary Antibody at 1:1000. Secondary Antibody at 1:1000. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: Control untreated cells. Lower Panel: Cells exposed to irradiation (10 Gy) and probed for 53BP1 foci. Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with primary antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa Fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - MUS81 inhibition in BRCA2-deficient cells causes accumulation of 53BP1 nuclear bodies and G1 arrest. H1299 cells carrying a DOX-inducible BRCA2 shRNA were transfected with control or MUS81 siRNAs. Representative images of cells processed 72 h later for immunostaining with 53BP1 Antibody (green) and anti-cyclin A (red) antibodies. DNA was counterstained with DAPI. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms15983), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Pot1a prevents DDR in HSCs. Telomeric DDR in 8 week-old LSK cells upon Pot1a knockdown. Immunocytochemical staining of TRF1 (green). Foci co-stained with TRF1 and 53BP1 were identified as TIFs. Nuclei were stained with TOTO3 (blue). Scale bar, 2 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00935-4), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Staining of 53BP1 in human colon cancer using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm, Po, Pm, Fe, Pm-Or, Pm, Rb, Sh
• Applications: WB, ChIP, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

53BP1 Antibody Summary

• Immunogen: The epitope recognized by 53BP1 Antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1].
• Marker: DNA Double Strand Break Marker
• Predicted Species: Porcine (90%), Primate (96%), Feline (90%), Chimpanzee (96%), Orangutan (96%), Rabbit (90%), Sheep (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:25000
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1.5 ug/ml
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation
  • Flow (Intracellular)
  • Knockdown Validated
  • Knockout Validated
• Application Notes: Immunohistochemistry-Paraffin (PMID: 27653664) and Immunohistochemistry (PMID: 24987917) were reported in scientific literature. Frozen section data from customer review. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 24591601). Use in Immunoprecipitation reported in scientific literature (PMID: 25645366). Knockout Validation was reported in scientific literature (PMID: 26601238). Use in Immunoblotting reported in multiple pieces of scientific literature.
• Theoretical MW: 214 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Rectum Whole Tissue Lysate (Adult Whole Tumor)
  Human Ovary Whole Tissue Lysate (Adult Whole Tumor)

Reactivity Notes

Human and mouse reactivity cited in numerous publications. Primate reactivity reported in scientific literature (PMID: 18389475). Fish reactivity reported in scientific literature (PMID: 25516420). Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). Predicted cross-reactivity based on sequence identity: Chimpanzee (96%), Gorilla (96%), Orangutan (96%), Gibbon (94%), Marmoset (92%), Feline (90%), Porcine (90%), Rabbit (90%), Sheep (90%)

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note for Lot A5, the dilution recommendations have changed from our previous lot recommendations. You may be required to use the antibody at a higher dilution than previous lots to obtain similar results. Please contact us if you have any questions.

Alternate Names for 53BP1 Antibody

• 53BP1
• p202
• p53-binding protein 1
• p53bp1
• TDRD30
• TP53-Binding Protein 1
• TP53BP1
• tumor protein 53-binding protein, 1
• tumor protein p53 binding protein 1
• tumor protein p53-binding protein, 1
• tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A.,... Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 53BP1 Antibody (NB100-304)(437)

Publications using NB100-304

• Wu J, Crowe DL Telomere DNA damage signaling regulates prostate cancer tumorigenesis Mol. Cancer Res. May 28 2020 [PMID: 32467172] (ICC/IF, Mouse)
• Bellelli R, Youds J, Borel V et al. Synthetic Lethality between DNA Polymerase Epsilon and RTEL1 in Metazoan DNA Replication Cell Rep May 26 2020 [PMID: 32460026] (ICC/IF, Mouse)
• Dan X, Babbar M, Moore A et al. DNA damage invokes mitophagy through a pathway involving Spata18 Nucleic Acids Res. May 26 2020 [PMID: 32453416] (ICC/IF, Human)
• Kelliher JL, West KL, Gong Q, Leung JWC Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination Nat Commun May 18 2020 [PMID: 32424115] (ICC/IF, Human)
• Hu Z, Mi S, Zhao T et al. BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites EMBO J. Apr 29 2020 [PMID: 32347575] (WB)
• Schochter F, Werner K, KOstler C et al. 53BP1 Accumulation in Circulating Tumor Cells Identifies Chemotherapy-Responsive Metastatic Breast Cancer Patients Cancers (Basel) Apr 9 2020 [PMID: 32283863] (WB, ICC/IF, Human)
• Gzil A, Jaworski D, Antosik P et al. The impact of TP53BP1 and MLH1 on metastatic capability in cases of locally advanced prostate cancer and their usefulness in clinical practice Urol. Oncol. Apr 10 2020 [PMID: 32280038] (IHC-P, Human)
• Ramadan R, Vromans E, Anang DC et al. Connexin43 Hemichannel Targeting With TAT-Gap19 Alleviates Radiation-Induced Endothelial Cell Damage Front Pharmacol Mar 5 2020 [PMID: 32210810] (ICC/IF, Human)
• Ferrara-Romeo I, Martinez P, Saraswati S et Al. The mTOR pathway is necessary for survival of mice with short telomeres Nat Commun Mar 3 2020 [PMID: 32127537] (ICC/IF, Mouse)
• Salvador-Barbero B, Alvarez-FernAndez M, Zapatero-Solana E et al. CDK4/6 Inhibitors Impair Recovery from Cytotoxic Chemotherapy in Pancreatic Adenocarcinoma Cancer Cell Mar 16 2020 [PMID: 32109375]
• Gauthier T, Duarte-Hospital C, Vignard J et al. Versicolorin A, a precursor in aflatoxins biosynthesis, is a food contaminant toxic for human intestinal cells Environ Int Feb 24 2020 [PMID: 32106047] (ICC/IF, Human)
• Chojnowski A, Ong PF, Foo MXR et al. Heterochromatin loss as a determinant of progerin-induced DNA damage in Hutchinson-Gilford Progeria Aging Cell Feb 22 2020 [PMID: 32087607] (ICC/IF, Human)
• Struve N, Binder ZA, Stead LF et al. EGFRvIII upregulates DNA mismatch repair resulting in increased temozolomide sensitivity of MGMT promoter methylated glioblastoma Oncogene Feb 17 2020 [PMID: 32066879] (ICC/IF, Human)
• Popp HD, Kohl V, Naumann N et al. DNA Damage and DNA Damage Response in Chronic Myeloid Leukemia Int J Mol Sci Feb 11 2020 [PMID: 32053969] (ICC/IF, Human)
• Belmans N, Gilles L, Vermeesen R et al. Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan Sci Rep Feb 7 2020 [PMID: 32034200] (ICC/IF, Human)
• Zelensky AN, Schoonakker M, Brandsma I et al. Low dose ionizing radiation strongly stimulates insertional mutagenesis in a gamma H2AX dependent manner PLoS Genet. Jan 1 2020 [PMID: 31945059] (IB, KO, Mouse)
• Riancho J, Castanedo-Vazquez D, Gil-Bea F et al. ALS-derived fibroblasts exhibit reduced proliferation rate, cytoplasmic TDP-43 aggregation and a higher susceptibility to DNA damage J. Neurol. Jan 14 2020 [PMID: 31938860]
• Yang J, Griffin P, Vera D et al. Erosion of the Epigenetic Landscape and Loss of Cellular Identity as a Cause of Aging in Mammals SSRN Journal 2019 Oct 03 (ICC/IF, Mouse)
• Current K, Meyer C, Magyar CE et al. Investigating PSMA-targeted radioligand therapy efficacy as a function of cellular PSMA levels and intra-tumoral PSMA heterogeneity Clin. Cancer Res. Jan 13 2020 [PMID: 31932492] (IHC-P, Mouse)
• KOse-Vogel N, Stengel S, Gardey E, et al. Transcriptional Suppression of the NLRP3 Inflammasome and Cytokine Release in Primary Macrophages by Low-Dose Anthracyclines Cells Dec 28 2019 [PMID: 31905600] (ICC/IF, Human)
• Crochemore C, FernAndez-Molina C, Montagne B, et al. CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence Nat Commun Dec 6 2019 [PMID: 31811121] (ICC/IF, Human)
• Santos-Barriopedro I, Li Y, Bahl S, Seto E HDAC8 affects MGMT levels in glioblastoma cell lines via interaction with the proteasome receptor ADRM1 Genes Cancer Dec 5 2019 [PMID: 31798765] (ICC/IF, Human)
• Janoshazi AK, Horton JK, Zhao ML et al. Shining Light on the Response to Repair Intermediates in DNA of Living Cells DNA Repair (Amst.) Nov 12 2019 [PMID: 31790865] (ICC/IF)
• El-Daher MT, Lemale J, Bruneau J et al. Chronic Intestinal Pseudo-Obstruction and Lymphoproliferative Syndrome as a Novel Phenotype Associated With Tetratricopeptide Repeat Domain 7A Deficiency Front Immunol Nov 7 2019 [PMID: 31787977] (IB, ICC/IF, Mouse, Human)
• Henry E, Souissi-Sahraoui I, Deynoux M et al. Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations Haematologica Nov 28 2019 [PMID: 31780635] (ICC/IF, Human)
• Scherthan H, Lee JH, Maus E et al. Nanostructure of Clustered DNA Damage in Leukocytes after In-Solution Irradiation with the Alpha Emitter Ra-223 Cancers (Basel) Nov 26 2019 [PMID: 31779276] (ICC/IF, Human)
• Aguado J, Sola-Carvajal A, Cancila V et al. Inhibition of DNA damage response at telomeres improves the detrimental phenotypes of Hutchinson-Gilford Progeria Syndrome Nat Commun Nov 18 2019 [PMID: 31740672] (ICC/IF, Human)
• Whittemore K, MartInez-Nevado E, Blasco MA Slower rates of accumulation of DNA damage in leukocytes correlate with longer lifespans across several species of birds and mammals Aging (Albany NY) Nov 15 2019 [PMID: 31730540] (IHC-P, Reptile, Avian)
• Munoz-Lorente MA, Cano-Martin AC, Blasco MA Mice with hyper-long telomeres show less metabolic aging and longer lifespans Nat Commun Oct 17 2019 [PMID: 31624261] (IHC-P, Mouse)
• Yang G, Komaki Y, Yoshida I, Ibuki Y Formaldehyde inhibits UV-induced phosphorylation of histone H2AX Toxicol In Vitro 2019 Oct 12 [PMID: 31614172] (ICC/IF, Human)
• Lu X, Tang M, Zhu Q et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival Nucleic Acids Res. 2019 Oct 15 [PMID: 31612207] (IP, KD, Human)
• Bishop DK Non-enzymatic roles of human RAD51 at stalled replication forks. Mason JM, Chan YL, Weichselbaum RW et al. Nat Commun. [PMID: 31562309] (ICC/IF, Human)
• Sambeat A, Ratajczak J, Joffraud M et al. Endogenous nicotinamide riboside metabolism protects against diet-induced liver damage Nat Commun Sep 20 2019 [PMID: 31541116] (ICC/IF, Mouse)
• Zatreanu D, Han Z, Mitter R et al. Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability Mol. Cell Aug 23 2019 [PMID: 31519522] (ICC/IF, Human)
• Parvin S, Ramirez-Labrada A, Aumann S et al. LMO2 Confers Synthetic Lethality to PARP Inhibition in DLBCL Cancer Cell Aug 12 2019 [PMID: 31447348] (IP, Human)
• Cameron BD, Traver G, Roland JT et al. Bcl2-Expressing Quiescent Type B Neural Stem Cells in the Ventricular-Subventricular Zone Are Resistant to Concurrent Temozolomide/X-Irradiation Stem Cells Dec 1 2019 [PMID: 31430423] (IHC-P, Mouse)
• Sundaravinayagam Devakumar, Rahjouei Ali, Andreani Matteo et al. 53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function. Cell Reports 2019 August 06 [PMID: 31390554] (WB, Mouse)
• Hill RJ, Crossan GP DNA cross-link repair safeguards genomic stability during premeiotic germ cell development Nat. Genet. Aug 1 2019 [PMID: 31367016] (ICC/IF, Mouse)
• Brandsma I, Sato K, van Rossum-Fikkert SE et al. HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis Cell Rep 2019 Jun 25 [PMID: 31242413] (IF, Mouse, Human)
• Xia Y, Pfeifer CR, Zhu K et al. Rescue of DNA damage after constricted migration reveals a mechano-regulated threshold for cell cycle J. Cell Biol. Jun 25 2019 [PMID: 31239284]
• Peng J, Tang L, Cai M et al. RECQL5 plays an essential role in maintaining genome stability and viability of triple-negative breast cancer cells Cancer Med Jun 23 2019 [PMID: 31231988] (ICC/IF, Human)
• Grill S, Bisht K, Tesmer VM et al. Two Separation-of-Function Isoforms of Human TPP1 Dictate Telomerase Regulation in Somatic and Germ Cells bioRxiv Jun 18 2019 [PMID: 31216472] (IP, Human)
• Liu Y, Efimova EV, Ramamurthy AKron SJ Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence J. Cell. Sci. 2019 Jul 01 [PMID: 31189537]
• Smith LR, Irianto J, Xia Y et al. Constricted migration modulates stem cell differentiation Mol. Biol. Cell Jul 22 2019 [PMID: 31188712] (ICC/IF, Mouse)
• Pladevall-Morera D, Munk S, Ingham A et al. Proteomic characterization of chromosomal common fragile site (CFS)-associated proteins uncovers ATRX as a regulator of CFS stability Nucleic Acids Res. 2019 Jun 10 [PMID: 31180492] (IF, Human)
• Wen HJ, Gao S, Wang Y et al. Myeloid cell-derived HB-EGF Drives Tissue Recovery After Pancreatitis Cell Mol Gastroenterol Hepatol May 21 2019 [PMID: 31125624] (IF, Mouse)
• Ge Y, Wu S, Zhang Z et al. Inhibition of p53 and/or AKT as a new therapeutic approach specifically targeting ALT cancers Protein Cell May 21 2019 [PMID: 31115790] (ICC/IF, Human)
• Cho S, Vashisth M, Abbas A et al. Mechanosensing by the Lamina Protects against Nuclear Rupture, DNA Damage, and Cell-Cycle Arrest Dev. Cell May 8 2019 [PMID: 31105008] (ICC/IF, Human)
• Campillo-Marcos I, Lazo PA Olaparib and ionizing radiation trigger a cooperative DNA-damage repair response that is impaired by depletion of the VRK1 chromatin kinase J. Exp. Clin. Cancer Res. May 17 2019 [PMID: 31101118] (ICC/IF, Human)
• The autophagy Protein Atg5 Plays a Crucial Role in the Maintenance and Reconstitution Ability of Hematopoietic Stem Cells Jung HE, Shim YR, Oh JE Immune Netw. [PMID: 31089439] (Flow, Mouse)
• Huang J, Zhang J, Bellani MA et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2 Cell Rep May 7 2019 [PMID: 31067464]
• Caron P, Pankotai T, Wiegant WW et al. WWP2 ubiquitylates RNA polymerase II for DNA-PK-dependent transcription arrest and repair at DNA breaks Genes Dev. May 2 2019 [PMID: 31048545] (ICC/IF, KD)
• Lagunas AM, Francis M, Maniar NB et al. Paracrine Interaction of Cancer Stem Cell Populations Is Regulated by the Senescence-Associated Secretory Phenotype (SASP) Mol. Cancer Res. May 1 2019 [PMID: 31043491] (IHC-P, Mouse)
• Prieto, I;Zambrano, A;Laso, J;Aranda, A;Samper, E;Monsalve, M; Early induction of senescence and immortalization in PGC-1 alpha-deficient mouse embryonic fibroblasts Free Radic. Biol. Med. Apr 25 2019 [PMID: 31029787] (ICC/IF, Mouse)
• Nadeau, S;Cheng, A;Colmegna, I;Rodier, F; Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures Methods Mol. Biol. Apr 25 2019 [PMID: 31020633] (ICC/IF, Mouse)
• Galindo-Campos, MA;Bedora-Faure, M;Farres, J;Lescale, C;Moreno-Lama, L;Martinez, C;Martin-Caballero, J;Ampurdanes, C;Aparicio, P;Dantzer, F;Cerutti, A;Deriano, L;Yelamos, J; Coordinated signals from the DNA repair enzymes PARP-1 and PARP-2 promotes B-cell development and function Cell Death Differ. Apr 17 2019 [PMID: 30996287] (ICC/IF, Mouse)
• Gupta, R;Li, W;Yan, XJ;Barrientos, J;Kolitz, JE;Allen, SL;Rai, K;Chiorazzi, N;Mongini, PKA; Mechanism for IL-15-Driven B Cell Chronic Lymphocytic Leukemia Cycling: Roles for AKT and STAT5 in Modulating Cyclin D2 and DNA Damage Response Proteins J. Immunol. Apr 15 2019 [PMID: 30988120] (WB, Flow, Human)
• West, KL;Kelliher, JL;Xu, Z;An, L;Reed, MR;Eoff, RL;Wang, J;Huen, MSY;Leung, JWC; LC8/DYNLL1 is a 53BP1 effector and regulates checkpoint activation Nucleic Acids Res. Apr 15 2019 [PMID: 30982887] (WB, Human)
• Zhang, M;Wang, B;Li, T;Liu, R;Xiao, Y;Geng, X;Li, G;Liu, Q;Price, CM;Liu, Y;Wang, F; Mammalian CST averts replication failure by preventing G-quadruplex accumulation Nucleic Acids Res. Apr 12 2019 [PMID: 30976812] (ICC/IF, Human)
• Michelini, F;Rossiello, F;d'Adda di Fagagna, F;Francia, S; RNase A treatment and reconstitution with DNA damage response RNA in living cells as a tool to study the role of non-coding RNA in the formation of DNA damage response foci Nat Protoc May 1 2019 [PMID: 30962605] (ICC/IF, Mouse)
• Jung HE, Shim YR, Oh JE. The autophagy Protein Atg5 Plays a Crucial Role in the Maintenance and Reconstitution Ability of Hematopoietic Stem Cells Cell Rep. [PMID: 30943404] (Flow, Mouse)
• Sekhar, KR;Wang, J;Freeman, ML;Kirschner, AN; Radiosensitization by enzalutamide for human prostate cancer is mediated through the DNA damage repair pathway PLoS ONE Apr 1 2019 [PMID: 30933998] (ICC/IF, Human)
• Gourzones, C;Bellanger, C;Lamure, S;Gadacha, OK;De Paco, EG;Vincent, L;Cartron, G;Klein, B;Moreaux, J; Antioxidant Defenses Confer Resistance to High Dose Melphalan in Multiple Myeloma Cells Cancers (Basel) Mar 28 2019 [PMID: 30925767] (ICC/IF, Human)
• Li S, Su W, Zhang C. Linear Double-stranded DNAs as Innovative Biological Parts to Implement Genetic Circuits in Mammalian Cells FEBS J. Mar 26 2019 [PMID: 30913358] (ICC/IF, Human)
• Belmans N, Gilles L, Virag P et al. Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations Dentomaxillofac Radiol Mar 26 2019 [PMID: 30912976] (ICC/IF, Human)
• Schiroli G, Conti A, Ferrari S et al. Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response Cell Stem Cell Mar 14 2019 [PMID: 30905619] (ICC/IF, Human)
• Qin B, Yu J, Nowsheen S et al. UFL1 promotes histone H4 ufmylation and ATM activation Nat Commun Mar 18 2019 [PMID: 30886146] (ICC/IF, Human)
• Efimova EV, Appelbe OK, Ricco N et al. O-GlcNAcylation enhances double strand break repair, promotes cancer cell proliferation and prevents therapy-induced senescence in irradiated tumors Mol. Cancer Res. Mar 18 2019 [PMID: 30885991] (ICC/IF, Human)
• Selis D, Pande Y, Smoczer C et al. Cytotoxicity and Genotoxicity of a New Intracanal Medicament, 2-hydroxyisocaproic Acid-An InVitro Study J Endod Mar 14 2019 [PMID: 30879773] (ICC/IF, Human)
• Peng Y, Liao Q, Tan W et al. The deubiquitylating enzyme USP15 regulates homologous recombination repair and cancer cell response to PARP inhibitors Nat Commun Mar 15 2019 [PMID: 30874560] (ICC/IF, Human)
• Linder B, Wehle A, Hehlgans S et al. Arsenic Trioxide and (-)-Gossypol Synergistically Target Glioma Stem-Like Cells via Inhibition of Hedgehog and Notch Signaling Cancers (Basel) Mar 12 2019 [PMID: 30871073] (ICC/IF, Human)
• Ly P, Brunner SF, Shoshani O et al. Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements Nat. Genet. Apr 1 2019 [PMID: 30833795] (ICC/IF, Human)
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• Schmidt JC, Zaug AJ, Cech TR et al. Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres Cell. 2016 Aug 10 [PMID: 27523609] (ICC/IF, Human)
• Taniguchi JB, Kondo K, Fujita K et al. RpA1 ameliorates symptoms of mutant ataxin-1 knock-in mice and enhances DNA damage repair. Hum. Mol. Genet. Aug 11 2016 [PMID: 27516390] (IHC, Mouse)
• Patel PL, Suram A, Mirani N et al. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc. Natl. Acad. Sci. U.S.A. Aug 23 2016 [PMID: 27503890] (ICC/IF, Human)
• Chua S, Lee FY, Chiang HJ et al. The cardioprotective effect of melatonin and exendin-4 treatment in a rat model of cardiorenal syndrome. J. Pineal Res. Jul 28 2016 [PMID: 27465663] (IHC, ICC/IF, Rat)
• Butterworth KT, Nicol JR, Ghita M et al. Preclinical evaluation of gold-DTDTPA nanoparticles as theranostic agents in prostate cancer radiotherapy. Nanomedicine (Lond). Aug 1 2016 [PMID: 27463088] (ICC/IF, Human)
• Lambrus BG, Daggubati V, Uetake Y et al. A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis J. Cell Biol. 2016 Jul 18 [PMID: 27432896] (WB, ICC/IF)
• Mao P, Liu J, Zhang Z et al. Homologous recombination-dependent repair of telomeric DSBs in proliferating human cells Nat Commun 2016 Jul 11 [PMID: 27396625] (ICC/IF)
• Fong CS, Mazo G, Das T et al. 53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis Elife 2016 Jul 16 [PMID: 27371829]
• Picco V, Coste I, Giraud-Panis MJ et al. ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2 Oncotarget Jul 19 2016 [PMID: 27366950] (ICC/IF)
• Parekh PR, Choudhuri R, Weyemi U et al. Evaluation of surrogate tissues as indicators of drug activity in a melanoma skin model. Cancer Med Jun 23 2016 [PMID: 27339860] (IHC)
• Jullien D, Vignard J, Fedor Y et al. Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells. J. Cell. Sci. May 20 2016 [PMID: 27206857] (ICC/IF, Human)
• Stankovic-Valentin N, Drzewicka K, Konig C et al. Redox regulation of SUMO enzymes is required for ATM activity and survival in oxidative stress. EMBO J. May 12 2016 [PMID: 27174643]
• Ho TLF, Guilbaud G, Blow JJ et al. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Reports. 2016 Apr 14 [PMID: 27149840] (IHC-P, Mouse)
• Kreienkamp R, Croke M, Neumann MA et al. Vitamin D receptor signaling improves Hutchinson-Gilford progeria syndrome cellular phenotypes. Oncotarget. Apr 27 2016 [PMID: 27145372] (WB, Human)
• Pauty J, Cote MF, Rodrigue A et al. Investigation of the DNA damage response to SFOM-0046, a new small-molecule drug inducing DNA double-strand breaks Sci Rep Mar 22 2016 [PMID: 27001483] (ICC/IF, Human)
• O'Hagan-Wong K, Nadeau S, Carrier-Leclerc A et al. Increased IL-6 secretion by aged human mesenchymal stromal cells disrupts hematopoietic stem and progenitor cells' homeostasis. Oncotarget 2016 Mar 22 [PMID: 26934440] (ICC/IF)
• Francia S, Cabrini M, Matti V et al. DICER, DROSHA and DNA damage-response RNAs are necessary for the secondary recruitment of DNA damage response factors J. Cell. Sci. Feb 16 2016 [PMID: 26906421] (ICC/IF, Human)
• Surface L. Functional analysis of the histone variant H2A.Z during lineage commitment. Thesis. 2014 May 30
• Turner R. Relationships between nuclear lamins and telomere biology in progeroid laminopathies and cancer. Thesis. 2015 (WB, ICC/IF, Human)
• Cekan P, Hasegawa K, Pan Y et al. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. Mol Biol Cell 2016 Apr 15 [PMID: 26864624] (WB)
• Nagy Z, Kalousi A, Furst A et al. Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs. PLoS Genet 2016 Feb [PMID: 26845027] (WB)
• Lescale C, Abramowski V, Bedora-Faure M et al. RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair. Nat Commun. 2016 Feb 02 [PMID: 26833222] (ICC/IF, Mouse)
• Martinez I, Garcia-Carpizo V, Guijarro T et al. Induction of DNA double-strand breaks and cellular senescence by human respiratory syncytial virus Virulence May 18 2016 [PMID: 26809688] (ICC/IF, Human)
• Zhang H, Liu H, Chen Y et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016 Jan 05 [PMID: 26727879] (WB, ICC/IF, Human)
• Chatalic KLS, Konijnenberg M, Nonnekens J. In Vivo Stabilization of a Gastrin-Releasing Peptide Re-ceptor Antagonist Enhances PET Imaging and Radionu-clide Therapy of Prostate Cancer in Preclinical Studies. Theranostics. 2016 Jan 01 [PMID: 26722377] (IHC-P, Mouse)
• Obermeier K, Sachsenweger J, Friedl TW et al. Heterozygous PALB2 c.1592delT mutation channels DNA double-strand break repair into error-prone pathways in breast cancer patients. Oncogene. 2015 Dec 07 [PMID: 26640152] (WB, ICC/IF, Human)
• Rall M, Kraft D, Volcic M et al. Impact of Charged Particle Exposure on Homologous DNA Double-Strand Break Repair in Human Blood-Derived Cells. Front Oncol 2015 [PMID: 26618143] (ICC/IF, Human)
• Han X, Liu Z, Jo MC et al. CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv 2015 Aug [PMID: 26601238] (ICC/IF)
• Li M, Huang R, Jiang X et al. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. PLoS ONE. 2015 Nov 25 [PMID: 26599493] (ICC/IF, Mouse)
• Werner E, Wang H, Doetsch PW. Role of Pro-inflammatory Cytokines in Radiation-Induced Genomic Instability in Human Bronchial Epithelial Cells. Radiat. Res. 2015 Nov 18 [PMID: 26579942] (ICC/IF, Human)
  
  Details:
  53BP1 antibody along with gamma-H2AX was used for the detection of DNA repair foci in irradiated Human Bronchial Epithelial Cells (HBEC3-KT) fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton™ X-100.
• Storch K, Cordes N. The impact of CDK9 on radiosensitivity, DNA damage repair and cell cycling of HNSCC cancer cells. Int. J. Oncol. 2015 Nov 13 [PMID: 26573875]
• Djuzenova CS, Zimmermann M, Katzer A et al. A prospective study on histone Y-H2AX and 53BP1 foci expression in rectal carcinoma patients: correlation with radiation therapy-induced outcome. BMC Cancer. 2015 Nov 06 [PMID: 26541290] (ICC/IF, Human)
• Markova E, Somsedikova A, Vasilyev S et al. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy. Int. J. Radiat. Biol. 2015 Nov 04 [PMID: 26537244] (ICC/IF, Human)
• Perez M, Munoz-Galvan S, Jimenez-Garcia MP et al. Efficacy of CDK4 inhibition against sarcomas depends on their levels of CDK4 and p16ink4 mRNA. Oncotarget 2015 Dec 1 [PMID: 26528855] (WB)
• Dalm SU, Nonnekens J, Doeswijk GN et al. Comparison of the Therapeutic Response to Treatment with a 177Lu-Labeled Somatostatin Receptor Agonist and Antagonist in Preclinical Models. J Nucl Med 2016 Feb [PMID: 26514177]
• Chaudhary P, Marshall TI, Currell FJ et al. Variations in the Processing of DNA Double-Strand Breaks Along 60-MeV Therapeutic Proton Beams. Int J Radiat Oncol Biol Phys 2015 Jul 29 [PMID: 26452569]
• Hehlgans S, Oppermann J, Reichert S et al. The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis. Radiat Oncol 2015 Sep 19 [PMID: 26383618] (ICC/IF, Human)
• Kubben N, Brimacombe KR, Donegan M et al. A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Methods 2015 Sep 01 [PMID: 26341717] (ICC/IF, Human)
• Weaver AN, Cooper TS, Rodriguez M et al. DNA double strand break repair defect and sensitivity to poly ADP-ribose polymerase (PARP) inhibition in human papillomavirus 16-positive head and neck squamous cell carcinoma. Oncotarget 2015 Sep 29 [PMID: 26336991] (ICC/IF)
• Chojnowski A, Ong PF, Wong ES et al. Progerin reduces LAP2alpha-telomere association in Hutchinson-Gilford progeria. Elife 2015 [PMID: 26312502]
• Lin H, Ha K, Lu G et al. Cdc14A and Cdc14B Redundantly Regulate DNA Double-Strand Break Repair. Mol Cell Biol 2015 Nov [PMID: 26283732] (Mouse)
• Durdik M, Kosik P, Gursky J et al. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and gammaH2AX foci in human lymphocytes. Cytometry A. 2015 Aug 04 [PMID: 26243567] (Flow, ICC/IF, Human)
• Ortega-Martinez S. Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress. Redox Biol 2015 Aug [PMID: 26160768] (ICC/IF)
• Petroni M, Sardina F, Heil C et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ 2016 Feb [PMID: 26068589] (WB)
• Fukuda Takayo, Wu Wenwen, Okada Maiko et al. Class I histone deacetylase inhibitors inhibit the retention of BRCA1 and 53BP1 at the site of DNA damage. Cancer Science 2015 [PMID: 26053117] (WB, Human)
• Beyreuther E, Karsch L, Laschinsky L. Radiobiological response to ultra-short pulsed megavoltage electron beams of ultra-high pulse dose rate. Int. J. Radiat. Biol. 2015 May 13 [PMID: 25968557] (ICC/IF, Human)
  
  Details:
  53BP1 antibody was used for ICC-IF staining of cytospun cells of two human squamous cell carcinoma cell lines of different radiosensitivity -FaDu/undifferentiated HNSCC of moderate radiosensitivity and SKX/moderately well differentiated HNSCC of higher radiosensitivity than FaDu. Immunostaining was performed 24 h after irradiation and primary antibody was used at 1:3000 dilution with detection via AF488 conjugated secondary antibody.
• Markiewicz E, Barnard S, Haines J et al. Nonlinear ionizing radiation-induced changes in eye lens cell proliferation, cyclin D1 expression and lens shape. Open Biol. 2015 Apr 01 [PMID: 25924630] (ICC/IF, WB, Human)
  
  Details:
  53BP1 antibody was used for WB and ICC-IF applications on human lens epithgelial cell line FHL124 exposed or not to low-dose IR up to 2.28 Gy. See full text for protocol details and the data images (Figure 2 and 5).
• Yu Q, Katlinskaya YV, Carbone CJ et al. DNA-Damage-Induced Type I Interferon Promotes Senescence and Inhibits Stem Cell Function Cell Rep 2015 Apr 21 [PMID: 25921537] (ICC/IF, Human)
• Boucher D, Vu T, Bain AL et al. Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice. FASEB J. 2015 Apr 27 [PMID: 25917330] (ICC/IF, Mouse)
  
  Details:
  Vimentin antibody used for IHC on mouse cryosections from wounds that were untreated and treated with an in vitro-engineered adipose dressing (Fig 1A and 1E).
• Zong D, Callen E, Pegoraro G et al. Ectopic expression of RNF168 and 53BP1 increases mutagenic but not physiological non-homologous end joining. Nucleic Acids Res. 2015 May 26 [PMID: 25916843] (WB, ICC/IF, Mouse)
• Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015 Apr 20 [PMID: 25895060] (ICC/IF, Human)
• Meir M, Galanty Y, Kashani L et al. The COP9 signalosome is vital for timely repair of DNA double-strand breaks Nucleic Acids Res. 2015 Apr 08 [PMID: 25855810] (WB, Human)
• Bol GM, Vesuna F, Xie M et al. Targeting DDX3 with a small molecule inhibitor for lung cancer therapy. EMBO Mol Med. 2015 Mar 27 [PMID: 25820276] (ICC/IF, Human)
• Lewinska A, Jarosz P, Czech J, Rzeszutek I. Capsaicin-induced genotoxic stress does not promote apoptosis in A549 human lung and DU145 prostate cancer cells Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2015 Feb 01 [PMID: 25813723] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used in ICC-IF staining application for the detection of 53BP1 foci on human lung A549 and DU145 prostate cancer cells which were treated or not with 5-250uM concentrations of Capsaicin for 24 hours (Fig. 5C). The immunoassay was performed on interphase nuclei and implicated - 20 minutes fixation of cells with 3.7% formaldehyde containing 0.1% Triton X-100 in PBS, blocking with 1%BSA-0.25% Triton X-100-PBS at RT for 30 minutes, incubation of cells with primary antibody at 1:200 dilution in blocking buffer for ON at 4C, detection with FITC-conjugated secondary antibody and Hoechst 33,342, analysis of data with reference to number of 53BP foci.
• Xu G, Chapman JR, Brandsma I et al. REV7 counteracts DNA double-strand break resection and affects PARP inhibition. Nature. 2015 Mar 23 [PMID: 25799992] (WB, Mouse)
• Augello MA, Berman-Booty LD, Carr R et al. Consequence of the tumor-associated conversion to cyclin D1b. EMBO Mol Med. 2015 Mar 18 [PMID: 25787974] (ICC/IF, Human)
• Papp SJ, Huber AL, Jordan SD et al. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. Elife. 2015 Mar 11 [PMID: 25756610] (WB, ICC/IF, IP, Human, Mouse)
• Mar FA, Debnath J, Stohr BA. Autophagy-Independent Senescence and Genome Instability Driven by Targeted Telomere Dysfunction Autophagy 2015 Mar 09 [PMID: 25751002] (ICC/IF, Human)
• Nakagawa Y, Sedukhina AS, Okamoto N et al. NF-kappaB signaling mediates acquired resistance after PARP inhibition. Oncotarget 2015 Feb 28 [PMID: 25686825]
• Boccardi V, Razdan N, Kaplunov J et al. Stn1 is critical for telomere maintenance and long-term viability of somatic human cells Aging Cell. 2015 Feb 14 [PMID: 25684230] (ICC/IF, Human)
• Aarts M, Bajrami I, Herrera-Abreu MT et al. Functional genetic screen identifies increased sensitivity to WEE1 inhibition in cells with defects in Fanconi Anaemia and HR pathways Mol. Cancer Ther. 2015 Feb 11 [PMID: 25673822] (ICC/IF, Human)
• Grabowska W, Kucharewicz K, Wnuk M et al. Curcumin induces senescence of primary human cells building the vasculature in a DNA damage and ATM-independent manner Age (Dordr). 2015 Feb 01 [PMID: 25649709] (ICC/IF)
  
  Details:
  53BP1 antibody used in ICC-IF application for the detection of DNA damage/DNA double-strand breaks during curcumin-induced senescence of human vascular smooth muscle cell /VSMCs and endothelial cells/Ecs. The assay involved - fixation of cells in 70 % ethanol, 1:500 dilution of primary antibody, detection of 53BP1 foci using Alexa 488 conjugated secondary antibody and DAPI (Fig 4).
• Gibbs-Seymour I, Markiewicz E, Bekker-Jensen S et al. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage Aging Cell. 2015 Jan 23 [PMID: 25645366] (IP, Human)
• Lewinska A, Wnuk M, Grabowska W et al. Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells Toxicol. Lett. 2015 Mar 18 [PMID: 25644192] (ICC/IF, Human)
• Torres G, Leheste JR, Ramos RL. Immunocytochemical localization of dna double-strand breaks in human and rat brain Neuroscience. 2015 Jan 27 [PMID: 25637486] (ICC/IF, Rat)
• Badie S, Carlos AR, Folio C et al. BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres EMBO J. 2015 Jan 12 [PMID: 25582120] (WB, ICC/IF, Mouse)
• Sykora P, Misiak M, Wang Y et al. DNA polymerase b deficiency leads to neurodegeneration and exacerbates Alzheimer disease phenotypes. Nucleic Acids Res 2014 Dec 30 [PMID: 25552414] (IHC-P, Mouse)
  
  Details:
  Using the DyLight 550 conjugated version of NB100-304, catalog number NB100-304R.
• Diotti R, Kalan S, Matveyenko A, Loayza D. DNA-directed Polymerase Subunits Play a Vital Role in Human Telomeric Overhang Processing. Mol. Cancer Res. 2014 Dec 17 [PMID: 25519149] (ICC/IF, Human)
• Mender I, Gryaznov S, Dikmen ZG et al. Induction of Telomere Dysfunction Mediated by the Telomerase Substrate Precursor 6-Thio-2'-Deoxyguanosine. Cancer Discov. 2014 Dec 16 [PMID: 25516420] (FISH)
• Ito H, Fujita K, Tagawa K et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. EMBO Mol Med. 2015 Jan 06 [PMID: 25510912] (IHC-P, Mouse)
• Herrera-Moyano Emilia, Moriel-Carretero Maria, Montelone Beth A, Aguilera A. The rem mutations in the ATP-binding groove of the Rad3/XPD helicase lead to Xeroderma pigmentosum-Cockayne syndrome-like phenotypes. PLoS Genet. 2014 Dec 11 [PMID: 25500814] (ICC/IF)
• Baranski OA, Kalinichenko VV, Adami GR. Increased FOXM1 expression can stimulate DNA repair in normal hepatocytes in vivo but also increases nuclear foci associated with senescence. Cell Prolif. 2014 Dec 05 [PMID: 25477198] (IHC-Fr, Mouse)
  
  Details:
  53BP1 antibody used at 1-1000 dilution for IHC-Fr application on 2% PFA fixed liver sections of C57BL/6 and Tg(TTR-FOXM1)1Rhc mice that were exposed to Diethylnitrosamine/DEN carcinogen (Figure 3 and 4).
• Uringa EJ, Baldeyron C, Odijk H et al. A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity. DNA Repair (Amst) 2015 Jan [PMID: 25463395]
• Baltanas FC, Valero J, Alonso JR et al. Nuclear Signs of Pre-neurodegeneration. Methods Mol. Biol. 2014 Nov 28 [PMID: 25431056] (ICC/IF, Mouse)
• Neumayer G, Nguyen Md. TPX2 Impacts Acetylation of Histone H4 at Lysine 16: Implications for DnA Damage Response PLoS OnE et al. 2014 Nov 04 [PMID: 25365214] (WB, ICC/IF, Human)
  
  Details:
  53BP antibody used for WB and ICC-IF on HeLa cells subjected to miRnA-mediated depletion of TPX2.
• Zimmermann M, Kibe T, Kabir S, De Lange T. TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. Genes Dev. 2014 Oct 24 [PMID: 25344324] (WB, ICC/IF, Mouse)
• Fumagalli M, Rossiello F, Mondello C, D'Adda Di Fagagna F. Stable Cellular Senescence Is Associated with Persistent DDR Activation. PLoS OnE. 2014 Oct 24 [PMID: 25340529] (ICC/IF, Human)
• Li Z, Wang H, Wang Y et al. Effect of Radiation Quality on Mutagenic Joining of Enzymatically-Induced DnA Double-Strand Breaks in Previously Irradiated Human Cells. Radiat. Res. 2014 Oct 20 [PMID: 25329962]
• Gazzaniga FS, Blackburn EH An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity Blood. 2014 Dec 10 [PMID: 25320237] (ICC/IF, KD, Human)
• Burdak-Rothkamm S, Rothkamm K, Mcclelland K et al. BRCA1, FAnCD2 and Chk1 are potential molecular targets for the modulation of a radiation-induced DnA damage response in bystander cells. Cancer Lett. 2014 Oct 07 [PMID: 25304378] (ICC/IF, Human)
• Tang M, Li Y, Zhang X et al. Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) promotes non-homologous end joining and inhibits homologous recombination repair upon DnA damage. J. Biol. Chem. 2014 Oct 07 [PMID: 25294876] (WB, ICC/IF, Human)
• Werner E, Wang H, Doetsch PW. Opposite Roles for p38MAPK-Driven Responses and Reactive Oxygen Species in the Persistence and Resolution of Radiation-Induced Genomic Instability. PLoS ONE. 2014 Oct 02 [PMID: 25271419] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF of radiation exposed HBEC-3KT cells - fixed in 4% paraformaldehyde, permeabilized with PBS-0.2% TritonX-100 (Figure 2).
• Roukos V, Burgess RC, Misteli T. Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc. 2014 Oct 01 [PMID: 25255091]
• Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014 Sep 23 [PMID: 25245017] (IHC-P, Mouse)
• Taggart LE, McMahon SJ, Currell FJ et al. The role of mitochondrial function in gold nanoparticle mediated radiosensitisation. Cancer Nanotechnol. 2014 Sep 22 [PMID: 25243023] (ICC/IF, Human)
• Pefani DE, Latusek R, Pires I et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat. Cell Biol. 2014 Sep 14 [PMID: 25218637]
• Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4. Terasawa M, Shinohara A, Shinohara M. PLoS Genet. [PMID: 25166505] (Flow, ICC/IF, Human)
• Santos J, Gil J. TRIM28/KAP1 regulates senescence. Immunol. Lett. 2014 Aug 23 [PMID: 25160591] (WB, ICC/IF, Human)
• Gole B, Baumann C, Mian E et al. Endonuclease G initiates DNA rearrangements at the MLL breakpoint cluster upon replication stress. Oncogene. 2014 Aug 18 [PMID: 25132265]
• Vassilopoulos A, Tominaga Y, Kim HS et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2014 Aug 04 [PMID: 25088202] (IHC-P, Mouse)
• Frescas David, de Lange Titia. Binding of TPP1 protein to TIN2 protein is required for POT1a,b protein-mediated telomere protection. J Biol Chem. 2014 Aug 29 [PMID: 25056954] (Mouse)
• Carlessi L, Poli EF, Bechi G et al. Functional and molecular defects of hiPSC-derived neurons from patients with ATM deficiency. Cell Death Dis 2014 Jul 18 [PMID: 25032865] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF on neurons derived from hiPSC /human-induced pluripotent stem cells generated from reprogrammed fibroblasts from ATM-null A-T patients and normal controls to pluripotency. Cells washed with 0.9% NaCl, air dried and fixed in 3%PFA, permeabilized in 0.5% Triton X100/PBS -5 min/4C and blocked with 5% BSA before primary labelling for staininng the nuclear foci. Figure 6e shows the IR-induced nuclear foci/53BP1 immunostaining.
• Chene G, Ouellet V, Rahimi K et al. DNA Damage Signaling and Apoptosis in Preinvasive Tubal Lesions of Ovarian Carcinoma. Int. J. Gynecol. Cancer 2014 Jul 01 [PMID: 24987917] (IHC-P, Human)
  
  Details:
  53BP1 antibody used at 1:10,000 dilution for IHC-P on a TMA containing benign fallopian tubes, serous tubal intraepithelial carcinoma (STIC), high-grade serous ovarian cancer (HGSC) from patients with STICs (associated ovarian cancer /AOC) from the same patient and HGSCs without STICs/non-AOC (FIGURE 1).
• Peng M, Xie J, Ucher A et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J. 2014 Jun 25 [PMID: 24966277] (WB, ICC/IF, Human, Mouse)
  
  Details:
  Figure 1G - WB of 53BP1 or FANCJ in U2OS cells stably expressing shRNA vectors to either control or 53BP1 (a or b) that were also transfected with siRNAs to Con or FANCJ. Figure 7 E immunofluorescence of cells with 53BP1 foci in untreated MEFs
• Naipal KA, Verkaik NS, Ameziane N et al. Functional ex vivo assay to select Homologous Recombination deficient breast tumors for PARP inhibitor treatment. Clin. Cancer Res. 2014 Jun 24 [PMID: 24963051] (IHC-P, Human)
  
  Details:
  For evaluating formation of ionizing radiation induced foci (IRIF), breast tumor tissue obtained from patients undergoing wide local excision or amputation for breast cancer were processed for IHC-P using anti-53BP1 (NB100-304) at 1/500 dilution. Figure S3 shows gamma-H2AX and 53BP1 nuclear foci indicate the induction of DSBs in tissue slices.
• Broering TJ, Alavattam KG, Sadreyev RI et al. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J. Cell Biol. 2014 Jun 09 [PMID: 24914237] (ICC/IF, Mouse)
  
  Details:
  Immunostaining of meiotic chromosome spreads (Figure 1D) and mid-pachytene spermatocytes with 53BP1 signal on XY chromatin (Figure 1E)
• Tubbs AT, Dorsett Y, Chan E et al. kAP-1 Promotes Resection of Broken DNA Ends Not Protected by Gamma-H2AX and 53BP1 in G1-Phase Lymphocytes. Mol. Cell. Biol. 2014 May 19 [PMID: 24842905] (ICC/IF, Mouse)
• Polato F, Callen E, Wong N et al. CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J. Exp. Med. 2014 May 19 [PMID: 24842372] (WB, ICC/IF, Mouse)
• Coker H, Brockdorff N. SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks. J. Cell. Sci. 2014 May 01 [PMID: 24790221] (ICC/IF, Mouse)
• Bazzi H, Anderson KV. Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc. Natl. Acad. Sci. U.S.A. 2014 Apr 15 [PMID: 24706806] (IHC-Fr, Mouse)
• Popuri V, Hsu J, Khadka P et al. Human RECQL1 participates in telomere maintenance. Nucleic Acids Res. 3/12/2014 [PMID: 24623817] (ICC/IF, Human)
• Leung JW, Agarwal P, Canny MD et al. Nucleosome Acidic Patch Promotes RNF168- and RING1B/BMI1-Dependent H2AX and H2A Ubiquitination and DNA Damage Signaling. PLoS Genet. 3/1/2014 [PMID: 24603765] (ICC/IF, Human)
• Zahn A, Eranki AK, Patenaude AM et al. Activation induced deaminase C-terminal domain links DNA breaks to end protection and repair during class switch recombination. Proc. Natl. Acad. Sci. U.S.A. 3/3/2014 [PMID: 24591601] (ChIP, Mouse)
• Lamkowski Andreas, Forcheron Fabien, Agay Diane et al. DNA damage focus analysis in blood samples of minipigs reveals acute partial body irradiation. PLoS One. 2014 Feb 03 [PMID: 24498326]
• Frescas D, de Lange T. et al. TRF2-tethered TIN2 can mediate telomere protection by TPP1/POT1. Mol. Cell. Biol. 2014 Jan 28 [PMID: 24469404] (ICC/IF, Mouse)
• Frescas D, de Lange T. et al. A TIN2 dyskeratosis congenita mutation causes telomerase-independent telomere shortening in mice. Genes Dev. 2014 Jan 22 [PMID: 24449270] (ICC/IF, Mouse)
• Chen B, Gilbert LA, Cimini BA et al. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 2013 Dec 19 [PMID: 24360272] (Human)
• Mariotti LG, Pirovano G, Savage KI et al. Use of the gamma-H2AX Assay to Investigate DNA Repair Dynamics Following Multiple Radiation Exposures. PLoS One 2013 Nov 29 [PMID: 24312182] (ICC/IF, Human)
• Bielak-Zmijewska A, Wnuk M, Przybylska D et al. A comparison of replicative senescence and doxorubicin-induced premature senescence of vascular smooth muscle cells isolated from human aorta. Biogerontology. 2013 Nov 16 [PMID: 24243065] (ICC/IF, Human)
• Mouron S, Rodriguez-Acebes S, Martinez-Jimenez MI et al. Repriming of DNA synthesis at stalled replication forks by human PrimPol. Nat Struct Mol Biol. 2013 Dec [PMID: 24240614] (ICC/IF, Human)
• Chan EA, Teng G, Corbett E et al. Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2. Proc Natl Acad Sci U S A. 2013 Nov 26 [PMID: 24218622] (ICC/IF, Mouse)
• Bekes M, Okamoto K, Crist SB et al. DUB-Resistant Ubiquitin to Survey Ubiquitination Switches in Mammalian Cells. Cell Rep. 2013 Nov 14 [PMID: 24210823] (ICC/IF, Mouse)
• Caganova M, Carrisi C, Varano G et al. Germinal center dysregulation by histone methyltransferase EZH2 promotes lymphomagenesis. J Clin Invest. 2013 Dec 2 [PMID: 24200695] (ICC/IF, Mouse)
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Reviews for 53BP1 Antibody (NB100-304) (13)

Average Rating: 4.6

(Based on 13 reviews)

We have 13 reviews tested in 3 species: Human, Mouse, Human and Mouse.

reviewed by: Anonymous ICC Human 05/24/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: IMR-90 human lung fibroblast cell line
• Species: Human
• Lot: D-6

reviewed by: Anonymous ICC Human 08/08/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Cancer Cells
• Species: Human
• Lot: E-4

Comments

• Comments: 1/1000 dilution

reviewed by: Anonymous IP Human 02/19/2019

Summary

• Application: Immunoprecipitation
• Sample Tested: human lung cancer cell line
• Species: Human
• Lot: E-6

reviewed by: Anonymous WB Mouse 11/27/2018

Summary

• Application: Western Blot
• Sample Tested: mouse epithelial pancreatic cancer cells
• Species: Mouse
• Lot: D-9 and D-10

Comments

• Comments: Membrane was blocked with 5% non-fat dry milk, 1h RT. Then incubated ON 4C with 53bp1 antibody 1:10000. After washes, membrane was incubated with HRP-conjugated secondary antibody diluted in 5% non-fat dry milk.

reviewed by: Vincent Picco ICC Human and Mouse 08/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured cell lines
• Species: Human and Mouse
• Lot: D-6

reviewed by: Anonymous IF Mouse 07/18/2017

Summary

• Application: Immunofluorescence
• Sample Tested: MEF
• Species: Mouse

reviewed by: Anonymous ICC Human 06/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: A172 Glioblastoma cells
• Species: Human
• Lot: D-2-092616-AF488

Comments

• Comments: Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with 53BP1 (AF488 conjugated) antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil).

reviewed by: Anonymous ICC Human 05/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: hela cell, fibroblasts
• Species: Human

reviewed by: Anonymous ICC Human 01/04/2016

Summary

• Application: Immunocytochemistry
• Species: Human
• Lot: multiple lots
• Special Applications: This antibody also works for western and immunofluorescence.
• File: View PDF

reviewed by: Anonymous WB Human 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23755068
• Species: Human

Blocking

• Blocking Details: See PMID 23755068

Details

• Detection Notes: See PMID 23755068

Comments

• Comments: Published in PMID: 23755068

reviewed by: leslie liberman IHC-Fr Mouse 01/21/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse cochlear spiral ganglion cells
• Species: Mouse
• Lot: 3
• Comments: This antibody co-localizes with anti-H2AX.

Blocking

• Blocking Details: 5% normal horse serum, one hr @ rm temp

Primary Anitbody

• Dilution Ratio: 1:500 in 1% normal horse serum with 0.3% triton-x, overnight at rm temp

Secondary Antibody

• Secondary Description: chicken anti-rabbit IgG, AlexaFluor488 conjugate, one hr at rm temp
• Secondary Manufacturer Cat#: Life Technologies #A21441
• Secondary Concentration: 1:1000, diluted in 1% normal horse serum

Details

• Detection Notes: Imaged on a Leica confocal. Tests on non-irradiated tissue, and secondaries-only incubation, produced no label.
• Fixation Details: 4% paraformaldehyde fixation, no antigen retrieval, permeabilized with 1% triton (in block), one hr @ rm temp
• Wash Description: PBS, 3 changes, 10 min. total

Comments

• Comments: This antibody co-localizes with anti-H2AX.

reviewed by: Anonymous IHC Mouse 07/14/2010

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse thymocytes
• Species: Mouse
• Comments: I needed to localize DSB repair at a single locus, so IHC was combined with DNA FISH. Many other antibodies failed, as DNA FISH requires heating the cells to 80 degrees C which denatures most proteins. This anti-53BP1 antibody resulted in discrete, bright foci regardless of FISH hybridization.

Comments

• Comments: I needed to localize DSB repair at a single locus, so IHC was combined with DNA FISH. Many other antibodies failed, as DNA FISH requires heating the cells to 80 degrees C which denatures most proteins. This anti-53BP1 antibody resulted in discrete, bright foci regardless of FISH hybridization.

reviewed by: Anonymous ICC Mouse 05/27/2009

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A

Blocking

• Blocking Details: Blocking Buffer: BSA, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Incubation Dilution Buffer: pbs + 0.2% BSA + 0.1% tween 20, Dilution Ratio: 1/500, Incubation Time: 1 hour, Incubation Temp: RT

Secondary Antibody

• Secondary Description: Secondary Ab: alexa fluor 633 goat anti rabbit, Secondary Ab Dilution: 1/500
• Secondary Manufacturer Cat#: invitrogen

FAQs for 53BP1 Antibody (NB100-304). (Showing 1 - 4 of 4 FAQs).

1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
   • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
   • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.
3. What is the recommended pH of sodium citrate for the 53bp1 antibody (NB100-304)?
   • I have checked our notes for use of this antibody in IHC, and they say: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
4. Is possible to know the exact sequence of the epitope used?
   • Unfortunately I am unable to disclose the sequence of this antibody due to proprietary restrictions. At the site recognized by NB100-304, the reference sequences are too divergent to expect NB100-304 to bind to either Bovine or Little Brown Bat. Regarding Mouse Homology, 30/34 sites of the immunogen are homologous Human - 100% homologous. If you would be interested in testing these novel species, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: TP53BP1