53BP1 Antibody

Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-53BP1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed Hela cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Data and image from confirmed customer review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - 53BP1 foci in proliferating MEFs; both normal and exposed to 10 Gy of IR.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Detection of Human and Mouse 53BP1 by IHC. Sample: FFPE sections of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: NB100-304 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

Flow Cytometry: 53BP1 Antibody [NB100-304] - 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 ug/ml anti-53BP1 NB100-304 in a 150 ul reaction. Isotype control (black), anti-53BP1 (red).

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Human breast tumors stained with 53BP1 antibody. Image from verified customer review.

Western Blot: 53BP1 Antibody [NB100-304] - Detection of 53BP1 by Western Blot Sample: Whole cell lysate from U2OS or 293T cells.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 conjugated to Alexa Fluor 488 [NB100-304AF488] at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - 53BP1 antibody was tested at 1:100 in HeLa cells with FITC (green). alpha-Tubulin antibody NB100-690 was tested at a 1:500 dilution using Mouse IgG secondary Antibody NB710-94914 at 1:1000 [HiLyte Fluor 555] (red). Nuclei were counterstained with Dapi (blue).

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 1 ug/mL of rabbit anti- 53BP1 polyclonal (NB100-304, Novus Biologicals). Cells were stained donkey anti-goat IgGNL557 and counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent Etoposide.Cells were immunostained for 53BP1 (green). The nuclei were counterstained with Dapi (blue). This image was submitted via customer Review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Embryonic Fibroblast cells Pre-extraction - 5mins with CSK buffer Fixed with 4% PFA and 75% Ethanol Primary Antibody - 1:1000 Secondary Antibody - 1:1000. This image was submitted via customer Review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-53BP1 conjugated to DyLight 550 [NB100-304R] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: Control untreated cells; Lower Panel: Cells exposed to Irradiation (10 Gy) and probed for 53BP1 foci. Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with primary antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil). This image was submitted via customer Review.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - IHC staining of 53BP1 in human colon cancer using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt, Fi, Gt, Pm
• Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC/IF, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

53BP1 Antibody Summary

• Immunogen: The epitope recognized by this antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1].
• Marker: DNA Double Strand Break Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:25000
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation
  • Immunocytochemistry 1:200
  • Knockout Validated
• Application Notes: This 53BP1 antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry and Flow Cytometry. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunohistochemistry-Paraffin and Immunohistochemistry were reported in scientific literature. Frozen section data from customer review. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24591601) Use in Immunoprecipitation reported in scientific literature (PMID 25645366). Knock Out Validation was reported in scientific literature (PMID: 26601238).
• Theoretical MW: 214 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Rectum Lysate (NB820-59448)
  Ovary Lysate (NB820-59442)

Reactivity Notes

Human and mouse cited in numerous publications. Primate reactivity reported in scientific literature (PMID: 18389475). Fish reactivity reported in scientific literature (PMID: 25516420).

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note for Lot A5, the dilution recommendations have changed from our previous lot recommendations. You may be required to use the antibody at a higher dilution than previous lots to obtain similar results. Please contact us if you have any questions.

Alternate Names for 53BP1 Antibody

• 53BP1
• MGC138366
• p202
• p53-binding protein 1
• p53BP1,53BP1FLJ41424
• TP53BP1
• tumor protein 53-binding protein, 1
• tumor protein p53 binding protein 1
• tumor protein p53-binding protein, 1
• tumor suppressor p53-binding protein 1

Background

53BP1 (p53 binding protein 1) plays a key role in response to DNA damage, checkpoint signaling during mitosis and enhancing TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 now has been established as a substrate for ATM (ataxia telangiectasia mutated) signaling and that it relocalizes to discrete foci overlapping with gamma H2AX (phosphorylated histone H2AX); demarcating DNA double strand breaks (DSBs) sites following exposure to radiation. 53BP1 functions downstream of gamma H2AX-dependent hierarchy of proteins that collectively establish IRIF (ionizing radiation induced foci) at DSBs; this hierarchy includes Mre11/Rad50/NBS1 (MRN complex), ATM, MDC1, RNF8, RNF168 and HERC2. With the exception of ATM, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-PK (DNA-dependent kinase), all of these proteins are physically and functionally required to recruit 53BP1 to the DSB site. Briefly, this process involves DSB recognition by MRN, ATM activation, gamma H2AX-formation, MDC1-recruitment, MRN-retention (leading to further ATM-activation and gamma H2AX spreading) and RNF8/RNF168/HERC2-mediated histone H2A and H2AX mono and poly-ubiquitination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 53BP1 Antibody (NB100-304)(317)

Publications using NB100-304

• Hehlgans S, Booms P, Gullulu O et al. Radiation Sensitization of Basal Cell and Head and Neck Squamous Cell Carcinoma by the Hedgehog Pathway Inhibitor Vismodegib Int J Mol Sci Aug 23 2018 [PMID: 30142876] (ICC/IF, Human)
• Muoio D, Berardinelli F, Leone S et al. Naphthalene diimide-derivatives G-quadruplex ligands induce cell proliferation inhibition, mild telomeric dysfunction and cell cycle perturbation in U251MG glioma cells FEBS J. Aug 10 2018 [PMID: 30095224] (ICC/IF, Human)
• de Carcer G, Venkateswaran SV, Salgueiro L et al. Plk1 overexpression induces chromosomal instability and suppresses tumor development Nat Commun Aug 1 2018 [PMID: 30069007] (Mouse)
• Evangelista FM, Maglott-Roth A, Stierle M et al. Transcription and mRNA export machineries SAGA and TREX-2 maintain monoubiquitinated H2B balance required for DNA repair J. Cell Biol. Jul 27 2018 [PMID: 30054449] (ICC/IF, Human)
• Ghezraoui H, Oliveira C, Becker JR et al. 53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ Nature Aug 1 2018 [PMID: 30046110] (WB, Mouse)
• Mirman Z, Lottersberger F, Takai H et al. 53BP1-RIF1-shieldin counteracts DSB resection through CST-and Pola-dependent fill-in. Nature. Jul 18 2018 [PMID: 30022158] (WB, Mouse)
• Genard G, Wera AC, Huart C et al. Proton irradiation orchestrates macrophage reprogramming through NFkB signaling Cell Death Dis Jun 27 2018 [PMID: 29950610] (ICC/IF, Human)
• Pecqueux C, Arslan A, Heller M et al. FGF-2 is a driving force for chromosomal instability and a stromal factor associated with adverse clinico-pathological features in prostate cancer Urol. Oncol. Jun 7 2018 [PMID: 29887238] (Human)
• Vallabhaneni H, Lynch PJ, Chen G et al. High Basal Levels of GH2AX in Human Induced Pluripotent Stem Cells are Linked to Replication-Associated DNA Damage and Repair Stem Cells Jun 6 2018 [PMID: 29873142] (Human)
• Canovas B, Igea A, Sartori AA et al. Targeting p38a Increases DNA Damage, Chromosome Instability, and the Anti-tumoral Response to Taxanes in Breast Cancer Cells Cancer Cell May 14 2018 [PMID: 29805078] (Human)
• Le O, Palacio L, Bernier G et al. INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice. Stem Cell Reports Apr 1 2018 [PMID: 29706499] (ICC/IF, Mouse)
• Biechonski S, Olender L, Zipin-Roitman A et al. Attenuated DNA damage responses and increased apoptosis characterize human hematopoietic stem cells exposed to irradiation. Sci Rep Apr 17 2018 [PMID: 29666389] (ICC/IF, Human)
• La Torre M, Merigliano C, Burla R et al. Mice with reduced expression of the telomere-associated protein Ft1 develop p53-sensitive progeroid traits. Aging Cell Apr 10 2018 [PMID: 29635765] (Mouse)
• Mitxelena J, Apraiz A, Vallejo-Rodriguez J et al. An E2F7-dependent transcriptional program modulates DNA damage repair and genomic stability. Nucleic Acids Res. Mar 24 2018 [PMID: 29590434] (ICC/IF)
• Bossuet-Greif N, Vignard J, Taieb F et al. The Colibactin Genotoxin Generates DNA Interstrand Cross-Links in Infected Cells. MBio. Mar 20 2018 [PMID: 29559578] (ICC/IF, Human)
• Massonneau J, Ouellet C, Lucien F et al. Suboptimal extracellular pH values alter DNA damage response to induced double-strand breaks. FEBS Open Bio. Mar 1 2018 [PMID: 29511618] (ICC/IF, Human)
• Castiglione MP, Ramos RL, Leheste JR, Torres G. Central and Peripheral Expression of DNA Double-Strand Breaks in Human and Mouse Tissues. Anat Rec (Hoboken). 2018 Feb 21 [PMID: 29466834] (Human)
• Yuan J, Liu Y, Wang J et al. Long-term Persistent Organic Pollutants Exposure Induced Telomere Dysfunction and Senescence-Associated Secretary Phenotype. J. Gerontol. A Biol. Sci. Med. Sci. Jul 9 2018 [PMID: 29360938] (ICC/IF, Human)
• Gavish-Izakson M, Velpula BB, Elkon R et al. Nuclear poly(A)-binding protein 1 is an ATM target and essential for DNA double-strand break repair. Nucleic Acids Res. 2017 Dec 14 [PMID: 29253183] (Human)
• Bill MA, Srivastava K, Breen C et al. Dual effects of radiation bystander signaling in urothelial cancer: purinergic-activation of apoptosis attenuates survival of urothelial cancer and normal urothelial cells. Oncotarget 2017 Nov 14 [PMID: 29228614] (ICC/IF, Human)
• Bill MA, Srivastava K, Breen C et al. Dual effects of radiation bystander signaling in urothelial cancer: purinergic-activation of apoptosis attenuates survival of normal and urothelial cancer cells Oncotarget. 2017 Nov 14 [PMID: 29228614] (ICC/IF, Human)
• Fujita K, Mao Y, Uchida S et al. Developmental YAPdeltaC determines adult pathology in a model of spinocerebellar ataxia type 1. Nat Commun 2017 Nov 30 [PMID: 29192206] (WB, Mouse)
• Michelini F, Pitchiaya S, Vitelli V et al. Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks Nat. Cell Biol. 2017 Dec 01 [PMID: 29180822] (ICC/IF)
• Jongen JMJ, van der Waals LM, Trumpi K et al. Downregulation of DNA repair proteins and increased DNA damage in hypoxic colon cancer cells is a therapeutically exploitable vulnerability Oncotarget. 2017 Oct 17 [PMID: 29156796] (WB, Human)
• Bejarano L, Schuhmacher AJ, Mendez M et al. Inhibition of TRF1 Telomere Protein Impairs Tumor Initiation and Progression in Glioblastoma Mouse Models and Patient-Derived Xenografts Cancer Cell. 2017 Nov 13 [PMID: 29136505] (Mouse)
• Vohhodina J, Barros EM, Savage AL et al. The RNA processing factors THRAP3 and BCLAF1 promote the DNA damage response through selective mRNA splicing and nuclear export Nucleic Acids Res. 2017 Nov 03 [PMID: 29112714] (WB, Human)
• Jones SE, Fleuren EDG, Frankum J et al. ATR is a therapeutic target in synovial sarcoma. Cancer Res. 2017 Oct 16 [PMID: 29038346] (ICC/IF, Human)
• Hosokawa K, MacArthur BD, Ikushima YM et al. The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age. Nat Commun. 2017 Oct 06 [PMID: 28986560] (Flow, Mouse)
• Schuhwerk H, Bruhn C, Siniuk K et al. Kinetics of poly (ADP-ribosyl) ation, but not PARP1 itself, determines the cell fate in response to DNA damage in vitro and in vivo Nucleic Acids Research 2017 Aug 18 [PMID: 28977496] (Mouse)
• Patel DS, Misenko SM, Her J, Bunting SF. BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites J. Cell Biol. 2017 Sep 14 [PMID: 28912125] (Human)
• Traver G, Mont S, Gius D et al. Loss of Nrf2 promotes alveolar type 2 cell loss in irradiated, fibrotic lung Free Radic. Biol. Med. 2017 Nov 01 [PMID: 28870520] (WB, Mouse)
• Perkhofer L, Schmitt A, Romero Carrasco MC et al. ATM deficiency generating genomic instability sensitizes pancreatic ductal adenocarcinoma cells to therapy-induced DNA damage Cancer Res. 2017 Aug 08 [PMID: 28790064] (Human)
• Lai X, Broderick R, Bergoglio V et al. MUS81 nuclease activity is essential for replication stress tolerance and chromosome segregation in BRCA2-deficient cells Nat Commun 2017 Jul 17 [PMID: 28714477] (ICC/IF, Human)
• Mikula-Pietrasik J, Uruski P, Pakula M et al. Oxidative stress contributes to hepatocyte growth factor-dependent pro-senescence activity of ovarian cancer cells. Free Radic. Biol. Med. 2017 Jun 23 [PMID: 28652056]
• Liu R, King A, Hoch NC et al. ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress. DNA Repair (Amst.). 2017 Jun 17 [PMID: 28648892] (WB, ICC/IF, Mouse)
• Zhang T, Zhang Z, Li F et al. Looping-out mechanism for resolution of replicative stress at telomeres. EMBO Rep. Aug 1 2017 [PMID: 28615293] (ICC/IF, Human)
• Yosef R, Pilpel N, Papismadov N et al. p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling. EMBO J. 2017 Jun 12 [PMID: 28607003] (ICC/IF, Human)
• Jaiswal H, Benada J, Mullers E et al. ATM/Wip1 activities at chromatin control Plk1 re-activation to determine G2 checkpoint duration. EMBO J. Jul 14 2017 [PMID: 28607002] (ICC/IF, Human)
• Garzon J, Rodriguez R, Kong Z et al. Shortage of dNTPs underlies altered replication dynamics and DNA breakage in the absence of the APC/C cofactor Cdh1. Oncogene. 2017 Jun 12 [PMID: 28604743] (Mouse)
• Ha K, Ma C, Lin H et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017 Jun 12 [PMID: 28604711] (ICC/IF, Human)
• Hilmi K, Jangal M, Marques M et al. CTCF facilitates DNA double-strand break repair by enhancing homologous recombination repair. Sci Adv May 1 2017 [PMID: 28560323] (WB, Human)
• Baselet Bjorn, Belmans Niels, Coninx Emma et al. Functional Gene Analysis Reveals Cell Cycle Changes and Inflammation in Endothelial Cells Irradiated with a Single X-ray Dose. Front Pharmacol 2017 [PMID: 28487652] (ICC/IF, Human)
• Latella L, Dall'Agnese A, Sesillo FB et al. DNA damage signaling mediates the functional antagonism between replicative senescence and terminal muscle differentiation. Genes Dev. Apr 1 2017 [PMID: 28446595]
• Huang D, Lu W, Zou S et al. Rho GDP-dissociation inhibitor alpha is a potential prognostic biomarker and controls telomere regulation in colorectal cancer Cancer Sci. 2017 Jul 01 [PMID: 28417530] (IHC-P, Human)
• Cottineau J, Kottemann MC, Lach FP et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J. Clin. Invest. May 1 2017 [PMID: 28414293] (ICC/IF, Human)
• Vancevska A, Douglass KM, Pfeiffer V et al. The telomeric DNA damage response occurs in the absence of chromatin decompaction. Genes Dev 2017 Mar 15 [PMID: 28381410] (WB, Human)
• Dixon CR, Platani M, Makarov AA, Schirmer EC. Microinjection of Antibodies Targeting the Lamin A/C Histone-Binding Site Blocks Mitotic Entry and Reveals Separate Chromatin Interactions with HP1, CenpB and PML. Cells. Mar 25 2017 [PMID: 28346356] (ICC/IF, Human)
• Yang TB, Chen Q, Deng JT et al. Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche. Nat Commun. Mar 17 2017 [PMID: 28303901] (ICC/IF, Mouse)
• Ji S, Zhu L, Gao Y et al. Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening. Cell Res. Mar 17 2017 [PMID: 28303890] (ICC/IF, Mouse)
• Nonnekens J, Chatalic KL, Molkenboer-Kuenen JD et al. (213)Bi-Labeled Prostate-Specific Membrane Antigen-Targeting Agents Induce DNA Double-Strand Breaks in Prostate Cancer Xenografts. Cancer Biother. Radiopharm. Mar 1 2017 [PMID: 28301262]
• Leung JW, Makharashvili N, Agarwal P et al. ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair Genes Dev. 2017 Feb 01 [PMID: 28242625] (Human)
• Rossiello F, Aguado J, Sepe S et al. DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs. Nat Commun. Feb 27 2017 [PMID: 28239143] (ICC/IF, Mouse)
• Liu Y, Cussiol JR, Dibitetto D et al. TOPBP1(Dpb11) plays a conserved role in homologous recombination DNA repair through the coordinated recruitment of 53BP1(Rad9). J. Cell Biol. 2017 Feb 22 [PMID: 28228534]
• Tan R, Nakajima S, Wang Q et al. Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1. Mol. Cell. 2017 Feb 08 [PMID: 28216227]
• Cheng LC, Tan VM, Ganesan S, Drake JM. Integrating phosphoproteomics into the clinical management of prostate cancer. Clin Transl Med 2017 Dec 01 [PMID: 28197968] (WB, ICC/IF)
• Reynolds JJ, Bicknell LS, Carroll P et al. Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism. Nat. Genet. 2017 Feb 13 [PMID: 28191891]
• Viziteu E, Klein B, Basbous J et al. RECQ1 helicase is involved in replication stress survival and drug resistance in multiple myeloma. Leukemia 2017 Mar 10 [PMID: 28186131]
• Kalimutho M, Bain AL, Mukherjee B et al. Enhanced dependency of KRAS mutant colorectal cancer cells on RAD51-dependent homologous recombination repair identified from genetic interactions in Saccharomyces cerevisiae. Mol Oncol. 2017 Feb 07 [PMID: 28173629]
• Feldman S, Wuerffel R, Achour I et al. 53BP1 Contributes to Igh Locus Chromatin Topology during Class Switch Recombination. J. Immunol. 2017 Feb 03 [PMID: 28159901] (ChIP, Mouse)
• Liu X, Liu F, Gao S et al. A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5. Cancer Letters 2017 Jan 27 [PMID: 28137631] (ICC/IF, Human)
• Mytych J, Wos I, Solek P, Koziorowski M. Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes. Exp Cell Res 2017 Jan 15 [PMID: 28011195] (Human)
• Tumini E, Barroso S, Aguilera A. Roles of human POLD1 and POLD3 in genome stability. Sci Rep. Dec 15 2016 [PMID: 27974823] (ICC/IF, Human)
• Wiedemann EM Peycheva M Pavri R. DNA Replication Origins in Immunoglobulin Switch Regions Regulate Class Switch Recombination in an R-Loop-Dependent Manner. Cell Rep. Dec 13 2016 [PMID: 27974207] (ChIP)
• Smirin-Yosef P, Zuckerman-Levin N, Tzur S et al. A biallelic mutation in the homologous recombination repair gene SPIDR is associated with human gonadal dysgenesis. J. Clin. Endocrinol. Metab. Dec 14 2016 [PMID: 27967308] (Human)
• Luo K, Li L, Li Y et al. A phosphorylation-deubiquitination cascade regulates the BRCA2-RAD51 axis in homologous recombination. Genes Dev. Dec 1 2016 [PMID: 27941124] (ICC/IF, Human)
• Jia P, Chastain M, Zou Y et al. Human MLH1 suppresses the insertion of telomeric sequences at intra-chromosomal sites in telomerase-expressing cells. Nucleic Acids Res. Nov 29 2016 [PMID: 27903905] (Human)
• Hess J, Unger K, Orth M et al. Genomic amplification of Fanconi anemia complementation group A (FancA) in head and neck squamous cell carcinoma (HNSCC): Cellular mechanisms of radioresistance and clinical relevance. Cancer Lett. Nov 17 2016 [PMID: 27867017] (Human)
• Hansen RK, Mund A, Poulsen SL et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat. Cell Biol. Dec 1 2016 [PMID: 27820601] (Mouse)
• Li M, Yu X, Chen Q. Chemopreventive effects of ROS targeting in a murine model of BRCA1-deficient breast cancer. Cancer Res. Nov 4 2016 [PMID: 27815389] (Human)
• Chang AC, Ong SG, LaGory EL et al. Telomere shortening and metabolic compromise underlie dystrophic cardiomyopathy. Proc. Natl. Acad. Sci. U.S.A. Oct 31 2016 [PMID: 27799523] (FLOW, Mouse)
• Bezine E, Malaise Y, Loeuillet A et al. Cell resistance to the Cytolethal Distending Toxin involves an association of DNA repair mechanisms. Sci Rep 2016 Oct 24 [PMID: 27775089]
• Toonen LJ, Schmidt I, Luijsterburg MS et al. Antisense oligonucleotide-mediated exon skipping as a strategy to reduce proteolytic cleavage of ataxin-3. Sci Rep Oct 12 2016 [PMID: 27731380]
• Sobiepanek A, Milner-Krawczyk M, Bobecka-Wesolowska K, Kobiela T The effect of delphinidin on the mechanical properties of keratinocytes exposed to UVB radiation. J. Photochem. Photobiol. B, Biol. Nov 1 2016 [PMID: 27716574] (Human)
• Przybylska Dorota, Janiszewska Dorota, Gozdzik Aleksandra et al. NOX4 downregulation leads to senescence of human vascular smooth muscle cells. Oncotarget 2016 [PMID: 27655718] (ICC/IF, Human)
• Imamura T, Fujita K, Tagawa K et al. Identification of hepta-histidine as a candidate drug for Huntington's disease by in silico-in vitro- in vivo-integrated screens of chemical libraries Sci Rep Sep 22 2016 [PMID: 27653664] (IHC, Mouse)
• Jakl L, Lobachevsky P, Vokalova L et al. Validation of JCountPro software for efficient assessment of ionizing radiation-induced foci in human lymphocytes. Int J Radiat Biol 2016 Dec [PMID: 27648492] (Human)
• Lescale C, Lenden Hasse H, Blackford A et al. Specific Roles of XRCC4 Paralogs PAXX and XLF during V(D)J Recombination. Cell Rep Sep 13 2016 [PMID: 27601299] (ICC/IF, Human)
• Bhatt N, Ghosh R, Roy S et al. Robust reprogramming of Ataxia-Telangiectasia patient and carrier erythroid cells to induced pluripotent stem cells. Stem Cell Res. Aug 12 2016 [PMID: 27596957] (ICC/IF)
• Croco E, Marchionni S, Bocchini M et al. DNA Damage Detection by 53BP1: Relationship to Species Longevity. J. Gerontol. A Biol. Sci. Med. Sci. 2016 Aug 29 [PMID: 27573809]
• Nonnekens J, van Kranenburg M, Beerens CE et al. Potentiation of Peptide Receptor Radionuclide Therapy by the PARP Inhibitor Olaparib. Theranostics. Aug 29 2016 [PMID: 27570553] (ICC/IF, Human)
• Henry A, Nokin Mj, Leroi N et al. New role of osteopontin in DNA repair and impact on Human glioblastoma radiosensitivity. Oncotarget Aug 22 2016 [PMID: 27563812]
• Graciotti M, Fang Z, Johnsson K, Gonczy P. Chemical genetic screen identifies natural products modulating centriole number. Chembiochem. Aug 25 2016 [PMID: 27558802] (Human)
• Montero JJ, Lopez de Silanes I, Grana O, Blasco MA. Telomeric RNAs are essential to maintain telomeres. Nat Commun. Aug 17 2016 [PMID: 27531349] (ICC/IF)
• Taniguchi JB, Kondo K, Fujita K et al. RpA1 ameliorates symptoms of mutant ataxin-1 knock-in mice and enhances DNA damage repair. Hum. Mol. Genet. Aug 11 2016 [PMID: 27516390] (IHC, Mouse)
• Patel PL, Suram A, Mirani N et al. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc. Natl. Acad. Sci. U.S.A. Aug 23 2016 [PMID: 27503890] (ICC/IF, Human)
• Chua S, Lee FY, Chiang HJ et al. The cardioprotective effect of melatonin and exendin-4 treatment in a rat model of cardiorenal syndrome. J. Pineal Res. Jul 28 2016 [PMID: 27465663] (IHC, ICC/IF, Rat)
• Butterworth KT, Nicol JR, Ghita M et al. Preclinical evaluation of gold-DTDTPA nanoparticles as theranostic agents in prostate cancer radiotherapy. Nanomedicine (Lond). Aug 1 2016 [PMID: 27463088] (ICC/IF, Human)
• Lambrus BG, Daggubati V, Uetake Y et al. A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis J. Cell Biol. 2016 Jul 18 [PMID: 27432896] (WB, ICC/IF)
• Mao P, Liu J, Zhang Z et al. Homologous recombination-dependent repair of telomeric DSBs in proliferating human cells Nat Commun 2016 Jul 11 [PMID: 27396625] (ICC/IF)
• Fong CS, Mazo G, Das T et al. 53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis Elife 2016 Jul 16 [PMID: 27371829]
• Picco V, Coste I, Giraud-Panis MJ et al. ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2 Oncotarget Jul 19 2016 [PMID: 27366950] (ICC/IF)
• Parekh PR, Choudhuri R, Weyemi U et al. Evaluation of surrogate tissues as indicators of drug activity in a melanoma skin model. Cancer Med Jun 23 2016 [PMID: 27339860] (IHC)
• Jullien D, Vignard J, Fedor Y et al. Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells. J. Cell. Sci. May 20 2016 [PMID: 27206857] (ICC/IF, Human)
• Stankovic-Valentin N, Drzewicka K, Konig C et al. Redox regulation of SUMO enzymes is required for ATM activity and survival in oxidative stress. EMBO J. May 12 2016 [PMID: 27174643]
• Ho TLF, Guilbaud G, Blow JJ et al. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Reports. 2016 Apr 14 [PMID: 27149840] (IHC-P, Mouse)
• Kreienkamp R, Croke M, Neumann MA et al. Vitamin D receptor signaling improves Hutchinson-Gilford progeria syndrome cellular phenotypes. Oncotarget. Apr 27 2016 [PMID: 27145372] (WB, Human)
• Pauty J, Cote MF, Rodrigue A et al. Investigation of the DNA damage response to SFOM-0046, a new small-molecule drug inducing DNA double-strand breaks Sci Rep Mar 22 2016 [PMID: 27001483] (ICC/IF, Human)
• O'Hagan-Wong K, Nadeau S, Carrier-Leclerc A et al. Increased IL-6 secretion by aged human mesenchymal stromal cells disrupts hematopoietic stem and progenitor cells' homeostasis. Oncotarget 2016 Mar 22 [PMID: 26934440] (ICC/IF)
• Francia S, Cabrini M, Matti V et al. DICER, DROSHA and DNA damage-response RNAs are necessary for the secondary recruitment of DNA damage response factors J. Cell. Sci. Feb 16 2016 [PMID: 26906421] (ICC/IF, Human)
• Cekan P, Hasegawa K, Pan Y et al. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. Mol Biol Cell 2016 Apr 15 [PMID: 26864624] (WB)
• Nagy Z, Kalousi A, Furst A et al. Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs. PLoS Genet 2016 Feb [PMID: 26845027] (WB)
• Lescale C, Abramowski V, Bedora-Faure M et al. RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair. Nat Commun. 2016 Feb 02 [PMID: 26833222] (ICC/IF, Mouse)
• Martinez I, Garcia-Carpizo V, Guijarro T et al. Induction of DNA double-strand breaks and cellular senescence by human respiratory syncytial virus Virulence May 18 2016 [PMID: 26809688] (ICC/IF, Human)
• Zhang H, Liu H, Chen Y et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016 Jan 05 [PMID: 26727879] (WB, ICC/IF, Human)
• Chatalic KLS, Konijnenberg M, Nonnekens J. In Vivo Stabilization of a Gastrin-Releasing Peptide Re-ceptor Antagonist Enhances PET Imaging and Radionu-clide Therapy of Prostate Cancer in Preclinical Studies. Theranostics. 2016 Jan 01 [PMID: 26722377] (IHC-P, Mouse)
• Obermeier K, Sachsenweger J, Friedl TW et al. Heterozygous PALB2 c.1592delT mutation channels DNA double-strand break repair into error-prone pathways in breast cancer patients. Oncogene. 2015 Dec 07 [PMID: 26640152] (WB, ICC/IF, Human)
• Rall M, Kraft D, Volcic M et al. Impact of Charged Particle Exposure on Homologous DNA Double-Strand Break Repair in Human Blood-Derived Cells. Front Oncol 2015 [PMID: 26618143] (ICC/IF, Human)
• Han X, Liu Z, Jo MC et al. CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv 2015 Aug [PMID: 26601238] (ICC/IF)
• Li M, Huang R, Jiang X et al. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. PLoS ONE. 2015 Nov 25 [PMID: 26599493] (ICC/IF, Mouse)
• Werner E, Wang H, Doetsch PW. Role of Pro-inflammatory Cytokines in Radiation-Induced Genomic Instability in Human Bronchial Epithelial Cells. Radiat. Res. 2015 Nov 18 [PMID: 26579942] (ICC/IF, Human)
  
  Details:
  53BP1 antibody along with gamma-H2AX was used for the detection of DNA repair foci in irradiated Human Bronchial Epithelial Cells (HBEC3-KT) fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton™ X-100.
• Storch K, Cordes N. The impact of CDK9 on radiosensitivity, DNA damage repair and cell cycling of HNSCC cancer cells. Int. J. Oncol. 2015 Nov 13 [PMID: 26573875]
• Djuzenova CS, Zimmermann M, Katzer A et al. A prospective study on histone Y-H2AX and 53BP1 foci expression in rectal carcinoma patients: correlation with radiation therapy-induced outcome. BMC Cancer. 2015 Nov 06 [PMID: 26541290] (ICC/IF, Human)
• Markova E, Somsedikova A, Vasilyev S et al. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy. Int. J. Radiat. Biol. 2015 Nov 04 [PMID: 26537244] (ICC/IF, Human)
• Perez M, Munoz-Galvan S, Jimenez-Garcia MP et al. Efficacy of CDK4 inhibition against sarcomas depends on their levels of CDK4 and p16ink4 mRNA. Oncotarget 2015 Dec 1 [PMID: 26528855] (WB)
• Dalm SU, Nonnekens J, Doeswijk GN et al. Comparison of the Therapeutic Response to Treatment with a 177Lu-Labeled Somatostatin Receptor Agonist and Antagonist in Preclinical Models. J Nucl Med 2016 Feb [PMID: 26514177]
• Chaudhary P, Marshall TI, Currell FJ et al. Variations in the Processing of DNA Double-Strand Breaks Along 60-MeV Therapeutic Proton Beams. Int J Radiat Oncol Biol Phys 2015 Jul 29 [PMID: 26452569]
• Hehlgans S, Oppermann J, Reichert S et al. The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis. Radiat Oncol 2015 Sep 19 [PMID: 26383618] (ICC/IF, Human)
• Kubben N, Brimacombe KR, Donegan M et al. A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Methods 2015 Sep 01 [PMID: 26341717] (ICC/IF, Human)
• Weaver AN, Cooper TS, Rodriguez M et al. DNA double strand break repair defect and sensitivity to poly ADP-ribose polymerase (PARP) inhibition in human papillomavirus 16-positive head and neck squamous cell carcinoma. Oncotarget 2015 Sep 29 [PMID: 26336991] (ICC/IF)
• Chojnowski A, Ong PF, Wong ES et al. Progerin reduces LAP2alpha-telomere association in Hutchinson-Gilford progeria. Elife 2015 [PMID: 26312502]
• Lin H, Ha K, Lu G et al. Cdc14A and Cdc14B Redundantly Regulate DNA Double-Strand Break Repair. Mol Cell Biol 2015 Nov [PMID: 26283732] (Mouse)
• Durdik M, Kosik P, Gursky J et al. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and gammaH2AX foci in human lymphocytes. Cytometry A. 2015 Aug 04 [PMID: 26243567] (Flow, ICC/IF, Human)
• Ortega-Martinez S. Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress. Redox Biol 2015 Aug [PMID: 26160768] (ICC/IF)
• Petroni M, Sardina F, Heil C et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ 2016 Feb [PMID: 26068589] (WB)
• Fukuda Takayo, Wu Wenwen, Okada Maiko et al. Class I histone deacetylase inhibitors inhibit the retention of BRCA1 and 53BP1 at the site of DNA damage. Cancer Science 2015 [PMID: 26053117] (WB, Human)
• Beyreuther E, Karsch L, Laschinsky L. Radiobiological response to ultra-short pulsed megavoltage electron beams of ultra-high pulse dose rate. Int. J. Radiat. Biol. 2015 May 13 [PMID: 25968557] (ICC/IF, Human)
  
  Details:
  53BP1 antibody was used for ICC-IF staining of cytospun cells of two human squamous cell carcinoma cell lines of different radiosensitivity -FaDu/undifferentiated HNSCC of moderate radiosensitivity and SKX/moderately well differentiated HNSCC of higher radiosensitivity than FaDu. Immunostaining was performed 24 h after irradiation and primary antibody was used at 1:3000 dilution with detection via AF488 conjugated secondary antibody.
• Markiewicz E, Barnard S, Haines J et al. Nonlinear ionizing radiation-induced changes in eye lens cell proliferation, cyclin D1 expression and lens shape. Open Biol. 2015 Apr 01 [PMID: 25924630] (ICC/IF, WB, Human)
  
  Details:
  53BP1 antibody was used for WB and ICC-IF applications on human lens epithgelial cell line FHL124 exposed or not to low-dose IR up to 2.28 Gy. See full text for protocol details and the data images (Figure 2 and 5).
• Yu Q, Katlinskaya YV, Carbone CJ et al. DNA-Damage-Induced Type I Interferon Promotes Senescence and Inhibits Stem Cell Function Cell Rep 2015 Apr 21 [PMID: 25921537] (ICC/IF, Human)
• Boucher D, Vu T, Bain AL et al. Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice. FASEB J. 2015 Apr 27 [PMID: 25917330] (ICC/IF, Mouse)
  
  Details:
  Vimentin antibody used for IHC on mouse cryosections from wounds that were untreated and treated with an in vitro-engineered adipose dressing (Fig 1A and 1E).
• Zong D, Callen E, Pegoraro G et al. Ectopic expression of RNF168 and 53BP1 increases mutagenic but not physiological non-homologous end joining. Nucleic Acids Res. 2015 May 26 [PMID: 25916843] (WB, ICC/IF, Mouse)
• Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015 Apr 20 [PMID: 25895060] (ICC/IF, Human)
• Meir M, Galanty Y, Kashani L et al. The COP9 signalosome is vital for timely repair of DNA double-strand breaks Nucleic Acids Res. 2015 Apr 08 [PMID: 25855810] (WB, Human)
• Bol GM, Vesuna F, Xie M et al. Targeting DDX3 with a small molecule inhibitor for lung cancer therapy. EMBO Mol Med. 2015 Mar 27 [PMID: 25820276] (ICC/IF, Human)
• Lewinska A, Jarosz P, Czech J, Rzeszutek I. Capsaicin-induced genotoxic stress does not promote apoptosis in A549 human lung and DU145 prostate cancer cells Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2015 Feb 01 [PMID: 25813723] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used in ICC-IF staining application for the detection of 53BP1 foci on human lung A549 and DU145 prostate cancer cells which were treated or not with 5-250uM concentrations of Capsaicin for 24 hours (Fig. 5C). The immunoassay was performed on interphase nuclei and implicated - 20 minutes fixation of cells with 3.7% formaldehyde containing 0.1% Triton X-100 in PBS, blocking with 1%BSA-0.25% Triton X-100-PBS at RT for 30 minutes, incubation of cells with primary antibody at 1:200 dilution in blocking buffer for ON at 4C, detection with FITC-conjugated secondary antibody and Hoechst 33,342, analysis of data with reference to number of 53BP foci.
• Xu G, Chapman JR, Brandsma I et al. REV7 counteracts DNA double-strand break resection and affects PARP inhibition. Nature. 2015 Mar 23 [PMID: 25799992] (WB, Mouse)
• Augello MA, Berman-Booty LD, Carr R et al. Consequence of the tumor-associated conversion to cyclin D1b. EMBO Mol Med. 2015 Mar 18 [PMID: 25787974] (ICC/IF, Human)
• Papp SJ, Huber AL, Jordan SD et al. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. Elife. 2015 Mar 11 [PMID: 25756610] (WB, ICC/IF, IP, Human, Mouse)
• Mar FA, Debnath J, Stohr BA. Autophagy-Independent Senescence and Genome Instability Driven by Targeted Telomere Dysfunction Autophagy 2015 Mar 09 [PMID: 25751002] (ICC/IF, Human)
• Nakagawa Y, Sedukhina AS, Okamoto N et al. NF-kappaB signaling mediates acquired resistance after PARP inhibition. Oncotarget 2015 Feb 28 [PMID: 25686825]
• Boccardi V, Razdan N, Kaplunov J et al. Stn1 is critical for telomere maintenance and long-term viability of somatic human cells Aging Cell. 2015 Feb 14 [PMID: 25684230] (ICC/IF, Human)
• Aarts M, Bajrami I, Herrera-Abreu MT et al. Functional genetic screen identifies increased sensitivity to WEE1 inhibition in cells with defects in Fanconi Anaemia and HR pathways Mol. Cancer Ther. 2015 Feb 11 [PMID: 25673822] (ICC/IF, Human)
• Grabowska W, Kucharewicz K, Wnuk M et al. Curcumin induces senescence of primary human cells building the vasculature in a DNA damage and ATM-independent manner Age (Dordr). 2015 Feb 01 [PMID: 25649709] (ICC/IF)
  
  Details:
  53BP1 antibody used in ICC-IF application for the detection of DNA damage/DNA double-strand breaks during curcumin-induced senescence of human vascular smooth muscle cell /VSMCs and endothelial cells/Ecs. The assay involved - fixation of cells in 70 % ethanol, 1:500 dilution of primary antibody, detection of 53BP1 foci using Alexa 488 conjugated secondary antibody and DAPI (Fig 4).
• Gibbs-Seymour I, Markiewicz E, Bekker-Jensen S et al. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage Aging Cell. 2015 Jan 23 [PMID: 25645366] (IP, Human)
• Lewinska A, Wnuk M, Grabowska W et al. Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells Toxicol. Lett. 2015 Mar 18 [PMID: 25644192] (ICC/IF, Human)
• Torres G, Leheste JR, Ramos RL. Immunocytochemical localization of dna double-strand breaks in human and rat brain Neuroscience. 2015 Jan 27 [PMID: 25637486] (ICC/IF, Rat)
• Badie S, Carlos AR, Folio C et al. BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres EMBO J. 2015 Jan 12 [PMID: 25582120] (WB, ICC/IF, Mouse)
• Sykora P, Misiak M, Wang Y et al. DNA polymerase b deficiency leads to neurodegeneration and exacerbates Alzheimer disease phenotypes. Nucleic Acids Res 2014 Dec 30 [PMID: 25552414] (IHC-P, Mouse)
  
  Details:
  Using the DyLight 550 conjugated version of NB100-304, catalog number NB100-304R.
• Diotti R, Kalan S, Matveyenko A, Loayza D. DNA-directed Polymerase Subunits Play a Vital Role in Human Telomeric Overhang Processing. Mol. Cancer Res. 2014 Dec 17 [PMID: 25519149] (ICC/IF, Human)
• Mender I, Gryaznov S, Dikmen ZG et al. Induction of Telomere Dysfunction Mediated by the Telomerase Substrate Precursor 6-Thio-2'-Deoxyguanosine. Cancer Discov. 2014 Dec 16 [PMID: 25516420] (FISH)
• Ito H, Fujita K, Tagawa K et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. EMBO Mol Med. 2015 Jan 06 [PMID: 25510912] (IHC-P, Mouse)
• Herrera-Moyano Emilia, Moriel-Carretero Maria, Montelone Beth A, Aguilera A. The rem mutations in the ATP-binding groove of the Rad3/XPD helicase lead to Xeroderma pigmentosum-Cockayne syndrome-like phenotypes. PLoS Genet. 2014 Dec 11 [PMID: 25500814] (ICC/IF)
• Baranski OA, Kalinichenko VV, Adami GR. Increased FOXM1 expression can stimulate DNA repair in normal hepatocytes in vivo but also increases nuclear foci associated with senescence. Cell Prolif. 2014 Dec 05 [PMID: 25477198] (IHC-Fr, Mouse)
  
  Details:
  53BP1 antibody used at 1-1000 dilution for IHC-Fr application on 2% PFA fixed liver sections of C57BL/6 and Tg(TTR-FOXM1)1Rhc mice that were exposed to Diethylnitrosamine/DEN carcinogen (Figure 3 and 4).
• Uringa EJ, Baldeyron C, Odijk H et al. A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity. DNA Repair (Amst) 2015 Jan [PMID: 25463395]
• Baltanas FC, Valero J, Alonso JR et al. Nuclear Signs of Pre-neurodegeneration. Methods Mol. Biol. 2014 Nov 28 [PMID: 25431056] (ICC/IF, Mouse)
• Neumayer G, Nguyen Md. TPX2 Impacts Acetylation of Histone H4 at Lysine 16: Implications for DnA Damage Response PLoS OnE et al. 2014 Nov 04 [PMID: 25365214] (WB, ICC/IF, Human)
  
  Details:
  53BP antibody used for WB and ICC-IF on HeLa cells subjected to miRnA-mediated depletion of TPX2.
• Zimmermann M, Kibe T, Kabir S, De Lange T. TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. Genes Dev. 2014 Oct 24 [PMID: 25344324] (WB, ICC/IF, Mouse)
• Fumagalli M, Rossiello F, Mondello C, D'Adda Di Fagagna F. Stable Cellular Senescence Is Associated with Persistent DDR Activation. PLoS OnE. 2014 Oct 24 [PMID: 25340529] (ICC/IF, Human)
• Li Z, Wang H, Wang Y et al. Effect of Radiation Quality on Mutagenic Joining of Enzymatically-Induced DnA Double-Strand Breaks in Previously Irradiated Human Cells. Radiat. Res. 2014 Oct 20 [PMID: 25329962]
• Burdak-Rothkamm S, Rothkamm K, Mcclelland K et al. BRCA1, FAnCD2 and Chk1 are potential molecular targets for the modulation of a radiation-induced DnA damage response in bystander cells. Cancer Lett. 2014 Oct 07 [PMID: 25304378] (ICC/IF, Human)
• Tang M, Li Y, Zhang X et al. Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) promotes non-homologous end joining and inhibits homologous recombination repair upon DnA damage. J. Biol. Chem. 2014 Oct 07 [PMID: 25294876] (WB, ICC/IF, Human)
• Werner E, Wang H, Doetsch PW. Opposite Roles for p38MAPK-Driven Responses and Reactive Oxygen Species in the Persistence and Resolution of Radiation-Induced Genomic Instability. PLoS ONE. 2014 Oct 02 [PMID: 25271419] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF of radiation exposed HBEC-3KT cells - fixed in 4% paraformaldehyde, permeabilized with PBS-0.2% TritonX-100 (Figure 2).
• Roukos V, Burgess RC, Misteli T. Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc. 2014 Oct 01 [PMID: 25255091]
• Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014 Sep 23 [PMID: 25245017] (IHC-P, Mouse)
• Taggart LE, McMahon SJ, Currell FJ et al. The role of mitochondrial function in gold nanoparticle mediated radiosensitisation. Cancer Nanotechnol. 2014 Sep 22 [PMID: 25243023] (ICC/IF, Human)
• Pefani DE, Latusek R, Pires I et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat. Cell Biol. 2014 Sep 14 [PMID: 25218637]
• Terasawa M, Shinohara A, Shinohara M. Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4. PLoS Genet. 2014 Aug 01 [PMID: 25166505] (ICC/IF, FLOW, Human)
  
  Details:
  Rabbit anti-53BP1 antibody used for indirect immunostaining on HeLaS cells. Cells were fixed with 4% paraformaldehyde for 20 min and then permeabilized with 0.2% (v/v) Triton X-100 for 10 min.
• Santos J, Gil J. TRIM28/KAP1 regulates senescence. Immunol. Lett. 2014 Aug 23 [PMID: 25160591] (WB, ICC/IF, Human)
• Gole B, Baumann C, Mian E et al. Endonuclease G initiates DNA rearrangements at the MLL breakpoint cluster upon replication stress. Oncogene. 2014 Aug 18 [PMID: 25132265]
• Vassilopoulos A, Tominaga Y, Kim HS et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2014 Aug 04 [PMID: 25088202] (IHC-P, Mouse)
• Frescas David, de Lange Titia. Binding of TPP1 protein to TIN2 protein is required for POT1a,b protein-mediated telomere protection. J Biol Chem. 2014 Aug 29 [PMID: 25056954] (Mouse)
• Carlessi L, Poli EF, Bechi G et al. Functional and molecular defects of hiPSC-derived neurons from patients with ATM deficiency. Cell Death Dis 2014 Jul 18 [PMID: 25032865] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF on neurons derived from hiPSC /human-induced pluripotent stem cells generated from reprogrammed fibroblasts from ATM-null A-T patients and normal controls to pluripotency. Cells washed with 0.9% NaCl, air dried and fixed in 3%PFA, permeabilized in 0.5% Triton X100/PBS -5 min/4C and blocked with 5% BSA before primary labelling for staininng the nuclear foci. Figure 6e shows the IR-induced nuclear foci/53BP1 immunostaining.
• Chene G, Ouellet V, Rahimi K et al. DNA Damage Signaling and Apoptosis in Preinvasive Tubal Lesions of Ovarian Carcinoma. Int. J. Gynecol. Cancer 2014 Jul 01 [PMID: 24987917] (IHC-P, Human)
  
  Details:
  53BP1 antibody used at 1:10,000 dilution for IHC-P on a TMA containing benign fallopian tubes, serous tubal intraepithelial carcinoma (STIC), high-grade serous ovarian cancer (HGSC) from patients with STICs (associated ovarian cancer /AOC) from the same patient and HGSCs without STICs/non-AOC (FIGURE 1).
• Peng M, Xie J, Ucher A et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J. 2014 Jun 25 [PMID: 24966277] (WB, ICC/IF, Human, Mouse)
  
  Details:
  Figure 1G - WB of 53BP1 or FANCJ in U2OS cells stably expressing shRNA vectors to either control or 53BP1 (a or b) that were also transfected with siRNAs to Con or FANCJ. Figure 7 E immunofluorescence of cells with 53BP1 foci in untreated MEFs
• Naipal KA, Verkaik NS, Ameziane N et al. Functional ex vivo assay to select Homologous Recombination deficient breast tumors for PARP inhibitor treatment. Clin. Cancer Res. 2014 Jun 24 [PMID: 24963051] (IHC-P, Human)
  
  Details:
  For evaluating formation of ionizing radiation induced foci (IRIF), breast tumor tissue obtained from patients undergoing wide local excision or amputation for breast cancer were processed for IHC-P using anti-53BP1 (NB100-304) at 1/500 dilution. Figure S3 shows gamma-H2AX and 53BP1 nuclear foci indicate the induction of DSBs in tissue slices.
• Broering TJ, Alavattam KG, Sadreyev RI et al. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J. Cell Biol. 2014 Jun 09 [PMID: 24914237] (ICC/IF, Mouse)
  
  Details:
  Immunostaining of meiotic chromosome spreads (Figure 1D) and mid-pachytene spermatocytes with 53BP1 signal on XY chromatin (Figure 1E)
• Tubbs AT, Dorsett Y, Chan E et al. kAP-1 Promotes Resection of Broken DNA Ends Not Protected by Gamma-H2AX and 53BP1 in G1-Phase Lymphocytes. Mol. Cell. Biol. 2014 May 19 [PMID: 24842905] (ICC/IF, Mouse)
• Polato F, Callen E, Wong N et al. CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J. Exp. Med. 2014 May 19 [PMID: 24842372] (WB, ICC/IF, Mouse)
• Coker H, Brockdorff N. SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks. J. Cell. Sci. 2014 May 01 [PMID: 24790221] (ICC/IF, Mouse)
• Bazzi H, Anderson KV. Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc. Natl. Acad. Sci. U.S.A. 2014 Apr 15 [PMID: 24706806] (IHC-Fr, Mouse)
• Popuri V, Hsu J, Khadka P et al. Human RECQL1 participates in telomere maintenance. Nucleic Acids Res. 3/12/2014 [PMID: 24623817] (ICC/IF, Human)
• Leung JW, Agarwal P, Canny MD et al. Nucleosome Acidic Patch Promotes RNF168- and RING1B/BMI1-Dependent H2AX and H2A Ubiquitination and DNA Damage Signaling. PLoS Genet. 3/1/2014 [PMID: 24603765] (ICC/IF, Human)
• Zahn A, Eranki AK, Patenaude AM et al. Activation induced deaminase C-terminal domain links DNA breaks to end protection and repair during class switch recombination. Proc. Natl. Acad. Sci. U.S.A. 3/3/2014 [PMID: 24591601] (ChIP, Mouse)
• Lamkowski Andreas, Forcheron Fabien, Agay Diane et al. DNA damage focus analysis in blood samples of minipigs reveals acute partial body irradiation. PLoS One. 2014 Feb 03 [PMID: 24498326]
• Frescas D, de Lange T. et al. TRF2-tethered TIN2 can mediate telomere protection by TPP1/POT1. Mol. Cell. Biol. 2014 Jan 28 [PMID: 24469404] (ICC/IF, Mouse)
• Frescas D, de Lange T. et al. A TIN2 dyskeratosis congenita mutation causes telomerase-independent telomere shortening in mice. Genes Dev. 2014 Jan 22 [PMID: 24449270] (ICC/IF, Mouse)
• Chen B, Gilbert LA, Cimini BA et al. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 2013 Dec 19 [PMID: 24360272] (Human)
• Mariotti LG, Pirovano G, Savage KI et al. Use of the gamma-H2AX Assay to Investigate DNA Repair Dynamics Following Multiple Radiation Exposures. PLoS One 2013 Nov 29 [PMID: 24312182] (ICC/IF, Human)
• Bielak-Zmijewska A, Wnuk M, Przybylska D et al. A comparison of replicative senescence and doxorubicin-induced premature senescence of vascular smooth muscle cells isolated from human aorta. Biogerontology. 2013 Nov 16 [PMID: 24243065] (ICC/IF, Human)
• Mouron S, Rodriguez-Acebes S, Martinez-Jimenez MI et al. Repriming of DNA synthesis at stalled replication forks by human PrimPol. Nat Struct Mol Biol. 2013 Dec [PMID: 24240614] (ICC/IF, Human)
• Chan EA, Teng G, Corbett E et al. Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2. Proc Natl Acad Sci U S A. 2013 Nov 26 [PMID: 24218622] (ICC/IF, Mouse)
• Bekes M, Okamoto K, Crist SB et al. DUB-Resistant Ubiquitin to Survey Ubiquitination Switches in Mammalian Cells. Cell Rep. 2013 Nov 14 [PMID: 24210823] (ICC/IF, Mouse)
• Caganova M, Carrisi C, Varano G et al. Germinal center dysregulation by histone methyltransferase EZH2 promotes lymphomagenesis. J Clin Invest. 2013 Dec 2 [PMID: 24200695] (ICC/IF, Mouse)
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Reviews for 53BP1 Antibody (NB100-304) (8)

Average Rating: 4.4

(Based on 8 reviews)

We have 8 reviews tested in 3 species: Human, Mouse, Human and Mouse.

reviewed by: Vincent Picco ICC Human and Mouse 08/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured cell lines
• Species: Human and Mouse
• Lot: D-6

reviewed by: Anonymous IF Mouse 07/18/2017

Summary

• Application: Immunofluorescence
• Sample Tested: MEF
• Species: Mouse

reviewed by: Anonymous ICC Human 05/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: hela cell, fibroblasts
• Species: Human

reviewed by: Anonymous ICC Human 01/04/2016

Summary

• Application: Immunocytochemistry
• Species: Human
• Lot: multiple lots
• Special Applications: This antibody also works for western and immunofluorescence.
• File: View PDF

reviewed by: Anonymous WB Human 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23755068
• Species: Human

Blocking

• Blocking Details: See PMID 23755068

Details

• Detection Notes: See PMID 23755068

Comments

• Comments: Published in PMID: 23755068

reviewed by: leslie liberman IHC-Fr Mouse 01/21/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse cochlear spiral ganglion cells
• Species: Mouse
• Lot: 3
• Comments: This antibody co-localizes with anti-H2AX.

Blocking

• Blocking Details: 5% normal horse serum, one hr @ rm temp

Primary Anitbody

• Dilution Ratio: 1:500 in 1% normal horse serum with 0.3% triton-x, overnight at rm temp

Secondary Antibody

• Secondary Description: chicken anti-rabbit IgG, AlexaFluor488 conjugate, one hr at rm temp
• Secondary Manufacturer Cat#: Life Technologies #A21441
• Secondary Concentration: 1:1000, diluted in 1% normal horse serum

Details

• Detection Notes: Imaged on a Leica confocal. Tests on non-irradiated tissue, and secondaries-only incubation, produced no label.
• Fixation Details: 4% paraformaldehyde fixation, no antigen retrieval, permeabilized with 1% triton (in block), one hr @ rm temp
• Wash Description: PBS, 3 changes, 10 min. total

Comments

• Comments: This antibody co-localizes with anti-H2AX.

reviewed by: Anonymous IHC Mouse 07/14/2010

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse thymocytes
• Species: Mouse
• Comments: I needed to localize DSB repair at a single locus, so IHC was combined with DNA FISH. Many other antibodies failed, as DNA FISH requires heating the cells to 80 degrees C which denatures most proteins. This anti-53BP1 antibody resulted in discrete, bright foci regardless of FISH hybridization.

Comments

• Comments: I needed to localize DSB repair at a single locus, so IHC was combined with DNA FISH. Many other antibodies failed, as DNA FISH requires heating the cells to 80 degrees C which denatures most proteins. This anti-53BP1 antibody resulted in discrete, bright foci regardless of FISH hybridization.

reviewed by: Anonymous ICC Mouse 05/27/2009

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A

Blocking

• Blocking Details: Blocking Buffer: BSA, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Incubation Dilution Buffer: pbs + 0.2% BSA + 0.1% tween 20, Dilution Ratio: 1/500, Incubation Time: 1 hour, Incubation Temp: RT

Secondary Antibody

• Secondary Description: Secondary Ab: alexa fluor 633 goat anti rabbit, Secondary Ab Dilution: 1/500
• Secondary Manufacturer Cat#: invitrogen

FAQs for 53BP1 Antibody (NB100-304). (Showing 1 - 4 of 4 FAQs).

1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
   • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
   • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.
3. What is the recommended pH of sodium citrate for the 53bp1 antibody (NB100-304)?
   • I have checked our notes for use of this antibody in IHC, and they say: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
4. Is possible to know the exact sequence of the epitope used?
   • Unfortunately I am unable to disclose the sequence of this antibody due to proprietary restrictions. At the site recognized by NB100-304, the reference sequences are too divergent to expect NB100-304 to bind to either Bovine or Little Brown Bat. Regarding Mouse Homology, 30/34 sites of the immunogen are homologous Human - 100% homologous. If you would be interested in testing these novel species, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: TP53BP1
• Entrez:
  • 7158(Human)
  • 27223(Mouse)
  • 296099(Rat)