53BP1 Antibody - BSA Free

Images

 
Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.
Genetic Strategies: Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 ...read more
Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Human breast tumors stained with 53BP1 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Detection of Human and Mouse 53BP1 by IHC. Sample: FFPE sections of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: 53BP1 Antibody ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent EPE. Cells ...read more
Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Immunoprecipitation: 53BP1 Antibody [NB100-304] - Immunoprecipitation analysis lysates from HCC44 cells in 1% NP40. Image from verified customer review.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Effect of RK-33 on radiation-induced DNA damage. A Immunofluorescence images showing 53BP1 and gamma-H2AX foci in A549 ...read more
Western Blot: 53BP1 Antibody [NB100-304] - Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS/GFP-lamin A ceels were pretreated with 10 um ATMi f for 1 h before exposure to IR (10 Gy, 1 h ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on Ntera2 cells with 53BP1 Antibody NB100-304 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and ...read more
Western Blot: 53BP1 Antibody [NB100-304] - Whole cell lysate from U2OS or 293T cells. Bands indicate an observed molecular weight of ~220 kDa and the theoretical molecular weight is 214 kDa.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: 53BP1 foci in proliferating MEFs. Lower Panel: 53BP1 foci in proliferating MEFs exposed to 10 Gy of IR.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Embryonic Fibroblast cells pre-extraction for 5 mins with CSK buffer. Fixed with 4% PFA and 75% Ethanol. Primary Antibody at 1:1000. Secondary ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: Control untreated cells. Lower Panel: Cells exposed to irradiation (10 Gy) and probed for 53BP1 foci. ...read more
Genetic Strategies: Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - MUS81 inhibition in BRCA2-deficient cells causes accumulation of 53BP1 nuclear bodies and G1 arrest. H1299 cells carrying ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Pot1a prevents DDR in HSCs. Telomeric DDR in 8 week-old LSK cells upon Pot1a knockdown. Immunocytochemical staining of ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS cells were transfected with siCTRL or siLMNA and subjected to laser ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Staining of 53BP1 in human colon cancer using DAB with hematoxylin counterstain.
Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on A431 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm, Po, Pm, Fe, Pm-Or, Rb, ShSpecies Glossary
Applications WB, ChIP, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

53BP1 Antibody - BSA Free Summary

Immunogen
The epitope recognized by 53BP1 Antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1].
Marker
DNA Double Strand Break Marker
Predicted Species
Porcine (90%), Chimpanzee (96%), Orangutan (96%), Rabbit (90%), Feline (90%), Sheep (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53BP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Flow (Intracellular)
  • Flow Cytometry 1.5 ug/ml
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation
  • In-situ Hybridization
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:5000-1:25000
Application Notes
Use in In-situ Hybridization reported in scientific literature (PMID:34988401).Immunohistochemistry-Paraffin (PMID: 27653664). and Immunohistochemistry (PMID: 24987917). were reported in scientific literature. Frozen section data from customer review. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 24591601).. Use in Immunoprecipitation reported in scientific literature (PMID: 25645366).. Knockout Validation was reported in scientific literature (PMID: 26601238).. Use in Immunoblotting reported in multiple pieces of scientific literature. Use in In-situ Hybridization reported in scientific literature (PMID:33122293)..
Theoretical MW
214 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Rectum Whole Tissue Lysate (Adult Whole Tumor)
Human Ovary Whole Tissue Lysate (Adult Whole Tumor)
Reviewed Applications
Read 14 Reviews rated 4.6
using
NB100-304 in the following applications:

Publications
Read Publications using
NB100-304 in the following applications:

  • 5 publications
  • 1 publication
  • 10 publications
  • IB
    2 publications
  • 338 publications
  • IF
    3 publications
  • IHC
    21 publications
  • 4 publications
  • 27 publications
  • IP
    8 publications
  • ISH
    2 publications
  • KD
    5 publications
  • KO
    1 publication
  • PLA
    1 publication
  • WB
    79 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32877678). Human and mouse reactivity cited in numerous publications. Primate reactivity reported in scientific literature (PMID: 18389475). . Fish reactivity reported in scientific literature (PMID: 25516420). . Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). . Predicted cross-reactivity based on sequence identity: Chimpanzee (96%). , Gorilla (96%). , Orangutan (96%). , Gibbon (94%). , Marmoset (92%). , Feline (90%). , Porcine (90%). , Rabbit (90%). , Sheep (90%).

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Please note for Lot A5, the dilution recommendations have changed from our previous lot recommendations. You may be required to use the antibody at a higher dilution than previous lots to obtain similar results. Please contact us if you have any questions.

Alternate Names for 53BP1 Antibody - BSA Free

  • 53BP1
  • p202
  • p53-binding protein 1
  • p53bp1
  • TDRD30
  • TP53-Binding Protein 1
  • TP53BP1
  • tumor protein 53-binding protein, 1
  • tumor protein p53 binding protein 1
  • tumor protein p53-binding protein, 1
  • tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
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Species: Hu
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Species: Hu, Mu
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Publications for 53BP1 Antibody (NB100-304)(570)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Avian, Drosophila melanogaster, Goat, Naked mole-rat, Porcine, Primate, Rabbit, Reptile.

We have publications tested in 15 applications: ChIP, FISH, Flow, IB, ICC/IF, IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KO, PLA, WB.


Filter By Application
ChIP
(5)
FISH
(1)
Flow
(10)
IB
(2)
ICC/IF
(338)
IF
(3)
IHC
(21)
IHC-Fr
(4)
IHC-P
(27)
IP
(8)
ISH
(2)
KD
(5)
KO
(1)
PLA
(1)
WB
(79)
All Applications
Filter By Species
Human
(302)
Mouse
(136)
Rat
(6)
Avian
(1)
Drosophila melanogaster
(1)
Goat
(1)
Naked mole-rat
(1)
Porcine
(1)
Primate
(1)
Rabbit
(1)
Reptile
(1)
All Species
Showing Publications 1 - 10 of 570. Show All 570 Publications.
Publications using NB100-304 Applications Species
Tang M, Chen Z, Wang C et al. Histone chaperone ASF1 acts with RIF1 to promote DNA end-joining in BRCA1-deficient cells The Journal of biological chemistry Apr 23 2022 [PMID: 35472331]
Aminu B, Fux J, Mallette E et al. Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity Biomolecules Mar 22 2022 [PMID: 35454069] (WB, Human) WB Human
Mitrentsi I, Lou J, Kerjouan A et al. Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations Molecular cell Apr 13 2022 [PMID: 35447083] (ICC/IF, Human) ICC/IF Human
Gallant K, Bektas A, Kaileh M et al. HiIDDD: a high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells Scientific reports Apr 15 2022 [PMID: 35428779] (ICC/IF, Human) ICC/IF Human
Gunther R, Pal A, Williams C et al. Alteration of Mitochondrial Integrity as Upstream Event in the Pathophysiology of SOD1-ALS Cells [PMID: 35406813] (ICC/IF, Human) ICC/IF Human
Fujita H, Ikeda M, Ui A et al. CHAMP1-POGZ counteracts the inhibitory effect of 53BP1 on homologous recombination and affects PARP inhibitor resistance Oncogene Apr 7 2022 [PMID: 35393543] (WB, ICC/IF, Human) WB, ICC/IF Human
Scherthan H, Wagner SQ, GrundhOfer J et al. Planar Proton Minibeam Irradiation Elicits Spatially Confined DNA Damage in a Human Epidermis Model Cancers Mar 17 2022 [PMID: 35326696] (IHC, Human) IHC Human
Chardon F, Japaridze A, Witt H et al. CENP-B-mediated DNA loops regulate activity and stability of human centromeres Molecular cell Mar 17 2022 [PMID: 35320753]
Goy E, Tomezak M, Facchin C et al. The out-of-field dose in radiation therapy induces delayed tumorigenesis by senescence evasion eLife Mar 18 2022 [PMID: 35302491] (ICC/IF, Mouse) ICC/IF Mouse
Lee D, Apelt K, Lee SO et al. ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination Nucleic acids research Mar 7 2022 [PMID: 35253893] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 570 Publications.

Reviews for 53BP1 Antibody (NB100-304) (14) 4.614

Average Rating: 4.6
(Based on 14 reviews)
We have 14 reviews tested in 3 species: Human, Mouse, Human and Mouse.

Reviews using NB100-304:
Filter by Applications
Immunocytochemistry
(8)
Immunoprecipitation
(1)
Western Blot
(2)
Immunofluorescence
(1)
Immunohistochemistry-Frozen
(1)
Immunohistochemistry
(1)
All Applications
Filter by Species
Human
(7)
Mouse
(6)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry 53BP1 NB100-304
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5
reviewed by:
Takahiro Ito
Immunocytochemistry Human 05/24/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedIMR-90 human lung fibroblast cell line
SpeciesHuman
LotD-6
Immunocytochemistry 53BP1 NB100-304
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5
reviewed by:
Ingrid MONTARRAS
Immunocytochemistry Human 08/08/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedCancer Cells
SpeciesHuman
LotE-4

Comments

Comments1/1000 dilution
Immunoprecipitation 53BP1 NB100-304
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5
reviewed by:
Pauline Jeannot
Immunoprecipitation Human 02/19/2019
View

Summary

ApplicationImmunoprecipitation
Sample Testedhuman lung cancer cell line
SpeciesHuman
LotE-6
  5
reviewed by:
Wade Baker
Immunocytochemistry Mouse 02/04/2019
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse B cell
SpeciesMouse
LotA
Western Blot 53BP1 NB100-304
Enlarge
5
reviewed by:
Sammy Ferri Borgogno
Western Blot Mouse 11/27/2018
View

Summary

ApplicationWestern Blot
Sample Testedmouse epithelial pancreatic cancer cells
SpeciesMouse
LotD-9 and D-10

Comments

CommentsMembrane was blocked with 5% non-fat dry milk, 1h RT. Then incubated ON 4C with 53bp1 antibody 1:10000. After washes, membrane was incubated with HRP-conjugated secondary antibody diluted in 5% non-fat dry milk.
Immunocytochemistry 53BP1 NB100-304
Enlarge
4
reviewed by:
Verified Customer
Immunocytochemistry Human and Mouse 08/23/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured cell lines
SpeciesHuman and Mouse
LotD-6
Immunofluorescence 53BP1 NB100-304
Enlarge
5
reviewed by:
Darshil Patel
Immunofluorescence Mouse 07/18/2017
View

Summary

ApplicationImmunofluorescence
Sample TestedMEF
SpeciesMouse
Immunocytochemistry 53BP1 NB100-304
Enlarge
5
reviewed by:
NIKHIL VAD
Immunocytochemistry Human 06/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedA172 Glioblastoma cells
SpeciesHuman
LotD-2-092616-AF488

Comments

CommentsCells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with 53BP1 (AF488 conjugated) antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil).
  5
reviewed by:
Grant Stewart
Immunocytochemistry Human 05/22/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedfibroblasts,hela cell
SpeciesHuman
Immunocytochemistry 53BP1 NB100-304
Enlarge
4
reviewed by:
Youwei Zhang
Immunocytochemistry Human 01/04/2016
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Lotmultiple lots
  4
reviewed by:
cynthia morimoto
Western Blot Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23755068
SpeciesHuman
Immunohistochemistry-Frozen 53BP1 NB100-304
Enlarge
4
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Mouse 01/21/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse cochlear spiral ganglion cells
SpeciesMouse
Lot3
  5
reviewed by:
Elizabeth
Immunohistochemistry Mouse 07/14/2010
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse thymocytes
SpeciesMouse
  4
reviewed by:
System Admin
Immunocytochemistry Mouse 05/27/2009
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse B cell
SpeciesMouse
LotA

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for 53BP1 Antibody (NB100-304). (Showing 1 - 4 of 4 FAQs).

  1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
    • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
  2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
    • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.
  3. What is the recommended pH of sodium citrate for the 53bp1 antibody (NB100-304)?
    • I have checked our notes for use of this antibody in IHC, and they say: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
  4. Is possible to know the exact sequence of the epitope used?
    • Unfortunately I am unable to disclose the sequence of this antibody due to proprietary restrictions. At the site recognized by NB100-304, the reference sequences are too divergent to expect NB100-304 to bind to either Bovine or Little Brown Bat. Regarding Mouse Homology, 30/34 sites of the immunogen are homologous Human - 100% homologous. If you would be interested in testing these novel species, please take a look at our Innovator's Reward program.

Control Lysate(s)

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Blogs on 53BP1.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Blocking 53BP1 Expression Lessens Tumor Development in BRCA1-Defective Mice
Our antibody database at Novus Biologicals provides research tools for the forefront of cancer research. Recently, a mouse study using 53BP1 and BRCA1 antibodies showed that deletion of 53BP1 greatly lessened the incidence of tumor development in mice...  Read full blog post.

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Recent Reviews

4.6
5
9
4
5
3
0
2
0
1
0

Takahiro Ito
05/24/2020
Application: Immunocytochemistry
Species: Human

Ingrid MONTARRAS
08/08/2019
Application: Immunocytochemistry
Species: Human

Pauline Jeannot
02/19/2019
Application: Immunoprecipitation
Species: Human

Bioinformatics

Gene Symbol TP53BP1