53BP1 Antibody - BSA Free

Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.

Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Human breast tumors stained with 53BP1 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Detection of Human and Mouse 53BP1 by IHC. Sample: FFPE sections of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: 53BP1 Antibody (Catalog #NB100-304) used at a dilution of 1:1000 (1ug/mL). Detection: DAB.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent EPE. Cells were immunostained for 53BP1 (green). The nuclei were counterstained with DAPI (blue). Image from verified customer review.

Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunoprecipitation: 53BP1 Antibody [NB100-304] - Immunoprecipitation analysis lysates from HCC44 cells in 1% NP40. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Effect of RK-33 on radiation-induced DNA damage. A Immunofluorescence images showing 53BP1 and gamma-H2AX foci in A549 cells after 2-Gy radiation and A549 cells pre-treated with 6 uM RK-33, 12 h before radiation. Overlap of 53BP1 and gamma-H2AX is seen in the merged picture of the co-immunofluorescence staining. Scale bar is 2 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.15252/emmm.201404368) licensed under a CC-BY licence.

Western Blot: 53BP1 Antibody [NB100-304] - Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS/GFP-lamin A ceels were pretreated with 10 um ATMi f for 1 h before exposure to IR (10 Gy, 1 h recovery). Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analyzed by immunoblotting using 53BP1 and GFP antibodies. WCE represents 1% input. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with 53BP1 Antibody conjugated to DyLight 550 (NB100-304R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on Ntera2 cells with 53BP1 Antibody NB100-304 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: 53BP1 Antibody [NB100-304] - Whole cell lysate from U2OS or 293T cells. Bands indicate an observed molecular weight of ~220 kDa and the theoretical molecular weight is 214 kDa.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: 53BP1 foci in proliferating MEFs. Lower Panel: 53BP1 foci in proliferating MEFs exposed to 10 Gy of IR.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Embryonic Fibroblast cells pre-extraction for 5 mins with CSK buffer. Fixed with 4% PFA and 75% Ethanol. Primary Antibody at 1:1000. Secondary Antibody at 1:1000. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: Control untreated cells. Lower Panel: Cells exposed to irradiation (10 Gy) and probed for 53BP1 foci. Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with primary antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa Fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - MUS81 inhibition in BRCA2-deficient cells causes accumulation of 53BP1 nuclear bodies and G1 arrest. H1299 cells carrying a DOX-inducible BRCA2 shRNA were transfected with control or MUS81 siRNAs. Representative images of cells processed 72 h later for immunostaining with 53BP1 Antibody (green) and anti-cyclin A (red) antibodies. DNA was counterstained with DAPI. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms15983), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Pot1a prevents DDR in HSCs. Telomeric DDR in 8 week-old LSK cells upon Pot1a knockdown. Immunocytochemical staining of TRF1 (green). Foci co-stained with TRF1 and 53BP1 were identified as TIFs. Nuclei were stained with TOTO3 (blue). Scale bar, 2 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00935-4), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. U2OS cells were transfected with siCTRL or siLMNA and subjected to laser micro-irradiation, fixed 15 min later and then processed for immunofluorescence with gamma-H2AX and 53BP1 antibodies. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody NB100-304 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody NB100-304 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with 53PB1 Antibody conjugated to DyLight 550 (NB100-304R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: 53BP1 Antibody [NB100-304] - Staining of 53BP1 in human colon cancer using DAB with hematoxylin counterstain.

Flow Cytometry: 53BP1 Antibody [NB100-304] - An intracellular stain was performed on A431 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm, Po, Pm, Fe, Pm-Or, Rb, Sh
• Applications: WB, ChIP, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

53BP1 Antibody - BSA Free Summary

• Immunogen: The epitope recognized by 53BP1 Antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1].
• Marker: DNA Double Strand Break Marker
• Predicted Species: Porcine (90%), Chimpanzee (96%), Orangutan (96%), Rabbit (90%), Feline (90%), Sheep (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Flow (Intracellular)
  • Flow Cytometry 1.5 ug/ml
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation
  • In-situ Hybridization
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:5000-1:25000
• Application Notes: Use in In-situ Hybridization reported in scientific literature (PMID:34988401).Immunohistochemistry-Paraffin (PMID: 27653664). and Immunohistochemistry (PMID: 24987917). were reported in scientific literature. Frozen section data from customer review. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 24591601).. Use in Immunoprecipitation reported in scientific literature (PMID: 25645366).. Knockout Validation was reported in scientific literature (PMID: 26601238).. Use in Immunoblotting reported in multiple pieces of scientific literature. Use in In-situ Hybridization reported in scientific literature (PMID:33122293)..
• Theoretical MW: 214 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Rectum Whole Tissue Lysate (Adult Whole Tumor)
  Human Ovary Whole Tissue Lysate (Adult Whole Tumor)

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32877678). Human and mouse reactivity cited in numerous publications. Primate reactivity reported in scientific literature (PMID: 18389475). . Fish reactivity reported in scientific literature (PMID: 25516420). . Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). . Predicted cross-reactivity based on sequence identity: Chimpanzee (96%). , Gorilla (96%). , Orangutan (96%). , Gibbon (94%). , Marmoset (92%). , Feline (90%). , Porcine (90%). , Rabbit (90%). , Sheep (90%).

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note for Lot A5, the dilution recommendations have changed from our previous lot recommendations. You may be required to use the antibody at a higher dilution than previous lots to obtain similar results. Please contact us if you have any questions.

Alternate Names for 53BP1 Antibody - BSA Free

• 53BP1
• p202
• p53-binding protein 1
• p53bp1
• TDRD30
• TP53-Binding Protein 1
• TP53BP1
• tumor protein 53-binding protein, 1
• tumor protein p53 binding protein 1
• tumor protein p53-binding protein, 1
• tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 53BP1 Antibody (NB100-304)(570)

Publications using NB100-304

• Tang M, Chen Z, Wang C et al. Histone chaperone ASF1 acts with RIF1 to promote DNA end-joining in BRCA1-deficient cells The Journal of biological chemistry Apr 23 2022 [PMID: 35472331]
• Aminu B, Fux J, Mallette E et al. Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity Biomolecules Mar 22 2022 [PMID: 35454069] (WB, Human)
• Mitrentsi I, Lou J, Kerjouan A et al. Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations Molecular cell Apr 13 2022 [PMID: 35447083] (ICC/IF, Human)
• Gallant K, Bektas A, Kaileh M et al. HiIDDD: a high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells Scientific reports Apr 15 2022 [PMID: 35428779] (ICC/IF, Human)
• Gunther R, Pal A, Williams C et al. Alteration of Mitochondrial Integrity as Upstream Event in the Pathophysiology of SOD1-ALS Cells [PMID: 35406813] (ICC/IF, Human)
• Fujita H, Ikeda M, Ui A et al. CHAMP1-POGZ counteracts the inhibitory effect of 53BP1 on homologous recombination and affects PARP inhibitor resistance Oncogene Apr 7 2022 [PMID: 35393543] (WB, ICC/IF, Human)
• Scherthan H, Wagner SQ, GrundhOfer J et al. Planar Proton Minibeam Irradiation Elicits Spatially Confined DNA Damage in a Human Epidermis Model Cancers Mar 17 2022 [PMID: 35326696] (IHC, Human)
• Chardon F, Japaridze A, Witt H et al. CENP-B-mediated DNA loops regulate activity and stability of human centromeres Molecular cell Mar 17 2022 [PMID: 35320753]
• Goy E, Tomezak M, Facchin C et al. The out-of-field dose in radiation therapy induces delayed tumorigenesis by senescence evasion eLife Mar 18 2022 [PMID: 35302491] (ICC/IF, Mouse)
• Lee D, Apelt K, Lee SO et al. ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination Nucleic acids research Mar 7 2022 [PMID: 35253893] (WB, ICC/IF, Human)
• Zhang T, Xu C, Zheng P et al. Glaucocalyxin B Attenuates Ovarian Cancer Cell Growth and Cisplatin Resistance In Vitro via Activating Oxidative Stress Oxidative Medicine and Cellular Longevity Feb 25 2022 [PMID: 35251480] (ICC/IF, Human)
• Dorman LC, Nguyen PT, Escoubas CC et al. A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosis bioRxiv : the preprint server for biology Feb 22 2022 [PMID: 35233577] (ICC/IF, Mouse)
• Setiaputra D, Escribano-DIaz C, Reinert JK et al. RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1 Molecular cell Feb 16 2022 [PMID: 35216668]
• Harhouri K, Cau P, Casey F et al. MG132 Induces Progerin Clearance and Improves Disease Phenotypes in HGPS-like Patients' Cells Cells Feb 10 2022 [PMID: 35203262] (ICC/IF, Human)
• Osborne H, Larrosa I, Schmidt C Sesquiterpene Lactones Potentiate Olaparib-Induced DNA Damage in p53 Wildtype Cancer Cells International Journal of Molecular Sciences Jan 20 2022 [PMID: 35163037] (ICC/IF, Human)
• Bosso G, Lanuza-Gracia P, PiNeiro-Hermida S et al. Early differential responses elicited by BRAFV600E in adult mouse models Cell death & disease Feb 10 2022 [PMID: 35145078] (IHC, Mouse)
• Dutto I, Gerhards J, Herrera A et al. Pathway-specific effects of ADSL deficiency on neurodevelopment eLife Feb 8 2022 [PMID: 35133277] (ICC/IF, Human)
• Groelly FJ, Porru M, Zimmer J et al. Anti-tumoural activity of the G-quadruplex ligand pyridostatin against BRCA1/2-deficient tumours EMBO molecular medicine Feb 2 2022 [PMID: 35107878] (WB, ICC/IF)
• Datta S, Patel M, Kashyap S et al. Chimeric chromosome landscapes of human somatic cell cultures show dependence on stress and regulation of genomic repeats by CGGBP1 Oncotarget Jan 17 2022 [PMID: 35070079] (ChIP, Human)
• Zhang L, Geng X, Wang F et al. 53BP1 regulates heterochromatin through liquid phase separation Nature communications Jan 18 2022 [PMID: 35042897]
• Mirman Z, Sasi NK, King A et al. 53BP1-shieldin-dependent DSB processing in BRCA1-deficient cells requires CST-Pol alpha-primase fill-in synthesis Nature cell biology Jan 1 2022 [PMID: 35027730] (WB)
• Zhou Z, Yin H, Suye S et al. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice Reproductive biology Jan 10 2022 [PMID: 35026551] (IHC, Mouse)
• Marty F, Bettamin L, Thouard A et al. Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity iScience Jan 1 2022 [PMID: 35024577]
• Song S, Ma D, Xu L et al. Low-intensity pulsed ultrasound-generated singlet oxygen induces telomere damage leading to glioma stem cell awakening from quiescence iScience Jan 21 2022 [PMID: 34988401] (ISH, Human)
• Bennett PV, Johnson AM, Ackerman SE et al. Dose-Rate Effects of Protons and Light Ions for DNA Damage Induction, Survival and Transformation in Apparently Normal Primary Human Fibroblasts Radiation research Dec 15 2021 [PMID: 34910217]
• Marsden CG, Das L, Nottoli TP Et al. Mouse Embryonic Fibroblasts Isolated From Nthl1 D227Y Knockin Mice Exhibit Defective DNA Repair and Increased Genome Instability DNA repair Nov 17 2021 [PMID: 34826736] (ICC/IF, Mouse)
• Wallis SS, Ventimiglia LN, Otigbah E Et al. The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair Developmental cell Nov 20 2021 [PMID: 34818527] (ICC/IF, Human)
• Chatzidoukaki O, Stratigi K, Goulielmaki E Et al. R-loops trigger the release of cytoplasmic ssDNAs leading to chronic inflammation upon DNA damage Science advances Nov 19 2021 [PMID: 34797720] (ICC/IF, Mouse)
• Zhu Q, Huang J, Huang H Et al. RNF19A-mediated ubiquitination of BARD1 prevents BRCA1/BARD1-dependent homologous recombination Nature communications Nov 17 2021 [PMID: 34789768] (WB, ICC/IF, Human)
• Wu J, Galvan KJ, Bogard RD Et al. DNA Double-strand Break Signaling Is a Therapeutic Target in Head and Neck Cancer Anticancer research Nov 1 2021 [PMID: 34732408]
• Yamamura Y, Kawamura Y, Oiwa Y Et al. Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain Inflammation and regeneration Nov 1 2021 [PMID: 34719407] (ICC/IF, Mouse, Naked mole-rat)
• Jiang H, Mei YF SARS-CoV-2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro Viruses Oct 13 2021 [PMID: 34696485] (WB, ICC/IF, Human)
• Li X, Li X, Xie C Et al. cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence Protein & cell Oct 22 2021 [PMID: 34676498] (ICC/IF, Human)
• Uruski P, Sepetowska A, Konieczna C Et al. Primary high-grade serous ovarian cancer cells are sensitive to senescence induced by carboplatin and paclitaxel in vitro Cellular & molecular biology letters Oct 21 2021 [PMID: 34674640] (ICC/IF, Human)
• Dibitetto D, Sims J, AscenCAo C et al. Intrinsic ATR signaling shapes DNA end resection and suppresses toxic DNA-PKcs signaling NAR Cancer Jun 1 2020
• Sanchez-Bailon MP, Choi SY, Dufficy ER Et al. Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair Nature communications Nov 2 2021 [PMID: 34728620] (ICC/IF, Human)
• Tanaka H, Kondo K, Fujita K Et al. HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology Communications biology Oct 11 2021 [PMID: 34635772] (WB, Mouse)
• Tirman S, Quinet A, Wood M Et al. Temporally distinct post-replicative repair mechanisms fill PRIMPOL-dependent ssDNA gaps in human cells Molecular cell Oct 7 2021 [PMID: 34624216] (Human)
• Moreira H, Guerra Liberal F, McMahon S, Prise K Characterization of a custom-made 241Am alpha-source for radiobiological studies Applied Radiation and Isotopes Sep 1 2021 [PMID: 34488046] (ICC/IF, Human)
• Dagg RA, Zonderland G, Lombardi EP Et al. A transcription-based mechanism for oncogenic beta-catenin-induced lethality in BRCA1/2-deficient cells Nature communications Aug 13 2021 [PMID: 34389725]
• Schimmel J, MuNoz-Subirana N, Kool H Et al. Small tandem DNA duplications result from CST-guided Pol alpha-primase action at DNA break termini Nature communications Aug 10 2021 [PMID: 34376693] (WB, Mouse)
• Suzuki Y, Wenwen W, Ohta T, Hayashi SI Molecular targeted drugs resistance impairs double-strand break repair and sensitizes ER-positive breast cancer to PARP inhibitors Breast cancer (Tokyo, Japan) Aug 3 2021 [PMID: 34346034] (ICC/IF)
• Su X, Wang J, Jiang L Et Al. PCNA inhibition enhances the cytotoxicity of beta-lapachone in NQO1-Positive cancer cells by augmentation of oxidative stress-induced DNA damage Cancer letters Jul 27 2021 [PMID: 34329742] (ICC/IF)
• Pal S, Nixon BR, Glennon MS Et al. Replication Stress Response Modifies Sarcomeric Cardiomyopathy Remodeling Journal of the American Heart Association Aug 3 2021 [PMID: 34323119] (IHC-Fr, Mouse)
• Ovejero S, Soulet C, Moriel-Carretero M The Alkylating Agent Methyl Methanesulfonate Triggers Lipid Alterations at the Inner Nuclear Membrane That Are Independent from Its DNA-Damaging Ability International journal of molecular sciences Jul 12 2021 [PMID: 34299079]
• Isobe Sy, Hiraga Si, Nagao K Et Al. Protein phosphatase 1 acts as a RIF1 effector to suppress DSB resection prior to Shieldin action Cell reports Jul 13 2021 [PMID: 34260925] (WB, ICC/IF)
• Yamamoto Y, Minami M, Yoshida K et al. Irradiation Accelerates Plaque Formation and Cellular Senescence in Flow-Altered Carotid Arteries of Apolipoprotein E Knock-Out Mice Journal of the American Heart Association Jul 20 2021 [PMID: 34227406] (IHC-P)
• Cong K, Peng M, Kousholt An Et Al. Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency Molecular cell Jun 26 2021 [PMID: 34216544]
• Luessing J, Sakhteh M, Sarai N et al. The nuclear kinesin KIF18B promotes 53BP1-mediated DNA double-strand break repair Cell reports Jun 29 2021 [PMID: 34192545]
• Gaillard S, Charasson V, Ribeyre C et al. KDM5A and KDM5B histone-demethylases contribute to HU-induced replication stress response and tolerance Biology Open May 15 2021 [PMID: 34184733]
• Kosar M, Giannattasio M, Piccini D et al. The human nucleoporin Tpr protects cells from RNA-mediated replication stress Nature communications Jun 24 2021 [PMID: 34168151] (ICC/IF, Human)
• Ihle M, Biber S, Schroeder IS et al. Impact of the interplay between stemness features, p53 and pol iota on replication pathway choices Nucleic acids research Jun 24 2021 [PMID: 34165573]
• Lezaja A, Panagopoulos A, Wen Y et al. RPA shields inherited DNA lesions for post-mitotic DNA synthesis Nature communications Jun 22 2021 [PMID: 34158486] (ICC/IF, Human)
• Xu X, Ni K, He Y et al. The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation Nature communications Jun 10 2021 [PMID: 34112784] (ChIP, Human)
• Becker J, Bonnet C, Clifford G et al. BARD1 links histone H2A Lysine-15 ubiquitination to initiation of BRCA1-dependent homologous recombination bioRxiv Jun 2 2020 (WB, Human)
• Peng H, Zhang S, Peng Y et al. Yeast Bromodomain Factor 1 and Its Human Homolog TAF1 Play Conserved Roles in Promoting Homologous Recombination Advanced science (Weinheim, Baden-Wurttemberg, Germany) May 30 2021 [PMID: 34056863] (ICC/IF, Human)
• Liu Y, Zhao X, Wang B et al. miR-376a Provokes Rectum Adenocarcinoma Via CTC1 Depletion-Induced Telomere Dysfunction Frontiers in Cell and Developmental Biology Apr 16 2021 [PMID: 33937245] (IHC, Human)
• Jegadesan NK, Branzei D DDX11 loss causes replication stress and pharmacologically exploitable DNA repair defects Proceedings of the National Academy of Sciences of the United States of America Apr 27 2021 [PMID: 33879618] (WB, Human)
• Grill S, Padmanaban S, Friedman A et al. TPP1 mutagenesis screens unravel shelterin interfaces and functions in hematopoiesis JCI insight Apr 6 2021 [PMID: 33822766] (ICC/IF, Mouse)
• Sorimachi Y, Karigane D, Ootomo Y et al. p38alpha plays differential roles in hematopoietic stem cell activity dependent on aging contexts The Journal of biological chemistry Mar 18 2021 [PMID: 33745970] (ICC/IF, Mouse)
• Sadideen D, Chen B, Basili M, Shaheen M Ubiquitin-Specific-Processing Protease 47, A Novel 53BP1 regulator bioRxiv Jan 28 2021 (WB)
• Blakemore D, Vilaplana-Lopera N, Almaghrabi R et al. MYBL2 and ATM suppress replication stress in pluripotent stem cells EMBO reports Mar 28 2021 [PMID: 33779025] (ICC/IF, Mouse)
• Uruski P, Mikula-Pietrasik J, Drzewiecki M et al. Diverse functional responses to high glucose by primary and permanent hybrid endothelial cells in vitro Journal of molecular and cellular cardiology Mar 14 2021 [PMID: 33731316] (ICC/IF, Human)
• He W, Azzi-Martin L, Velasco V et al. The CDT of Helicobacter hepaticus induces pro-survival autophagy and nucleoplasmic reticulum formation concentrating the RNA binding proteins UNR/CSDE1 and P62/SQSTM1 PLoS pathogens Mar 1 2021 [PMID: 33662035] (IHC-P, Human)
• Zech HBAA Vergleich der DNA-Doppelstrangbruch-ReparaturkapazitAt HPV-positiver und negativer Oropharynxkarzinome im Ex-vivo-Assay Thesis Jan 1 2020
• Scaffa AM, Peterson AL, Carr JF et al. Hyperoxia causes senescence and increases glycolysis in cultured lung epithelial cells Physiological reports May 1 2021 [PMID: 34042288] (ICC/IF, Mouse)
• Tamayo-Orrego L, Gallo D, Racicot F et al. Sonic hedgehog accelerates DNA replication to cause replication stress promoting cancer initiation in medulloblastoma Nat Cancer Jul 20 2020
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  Details:
  A wester blot was performed using the 53BP1 antibody in WT and independent AHNAK-/- U2OS human cell lines. The interactions shown on multiple western blots determined that AHNAK restrains 53BP1-mediated p53 activation.
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  Details:
  NIH/3T3 cells and HEK292T cells (both treated with CX-5461 and untreated) underwent immunofluorescence microscopy
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  Details:
  Citation using the Janiela Fluor 549 format of this antibody.
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• Schmidt JC, Zaug AJ, Cech TR et al. Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres Cell. 2016 Aug 10 [PMID: 27523609] (ICC/IF, Human)
• Taniguchi JB, Kondo K, Fujita K et al. RpA1 ameliorates symptoms of mutant ataxin-1 knock-in mice and enhances DNA damage repair. Hum. Mol. Genet. Aug 11 2016 [PMID: 27516390] (IHC, Mouse)
• Patel PL, Suram A, Mirani N et al. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc. Natl. Acad. Sci. U.S.A. Aug 23 2016 [PMID: 27503890] (ICC/IF, Human)
• Chua S, Lee FY, Chiang HJ et al. The cardioprotective effect of melatonin and exendin-4 treatment in a rat model of cardiorenal syndrome. J. Pineal Res. Jul 28 2016 [PMID: 27465663] (IHC, ICC/IF, Rat)
• Butterworth KT, Nicol JR, Ghita M et al. Preclinical evaluation of gold-DTDTPA nanoparticles as theranostic agents in prostate cancer radiotherapy. Nanomedicine (Lond). Aug 1 2016 [PMID: 27463088] (ICC/IF, Human)
• Lambrus BG, Daggubati V, Uetake Y et al. A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis J. Cell Biol. 2016 Jul 18 [PMID: 27432896] (WB, ICC/IF)
• Mao P, Liu J, Zhang Z et al. Homologous recombination-dependent repair of telomeric DSBs in proliferating human cells Nat Commun 2016 Jul 11 [PMID: 27396625] (ICC/IF)
• Fong CS, Mazo G, Das T et al. 53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis Elife 2016 Jul 16 [PMID: 27371829]
• Picco V, Coste I, Giraud-Panis MJ et al. ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2 Oncotarget Jul 19 2016 [PMID: 27366950] (ICC/IF)
• Parekh PR, Choudhuri R, Weyemi U et al. Evaluation of surrogate tissues as indicators of drug activity in a melanoma skin model. Cancer Med Jun 23 2016 [PMID: 27339860] (IHC)
• Jullien D, Vignard J, Fedor Y et al. Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells. J. Cell. Sci. May 20 2016 [PMID: 27206857] (ICC/IF, Human)
• Stankovic-Valentin N, Drzewicka K, Konig C et al. Redox regulation of SUMO enzymes is required for ATM activity and survival in oxidative stress. EMBO J. May 12 2016 [PMID: 27174643]
• Ho TLF, Guilbaud G, Blow JJ et al. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Reports. 2016 Apr 14 [PMID: 27149840] (IHC-P, Mouse)
• Kreienkamp R, Croke M, Neumann MA et al. Vitamin D receptor signaling improves Hutchinson-Gilford progeria syndrome cellular phenotypes. Oncotarget. Apr 27 2016 [PMID: 27145372] (WB, Human)
• Pauty J, Cote MF, Rodrigue A et al. Investigation of the DNA damage response to SFOM-0046, a new small-molecule drug inducing DNA double-strand breaks Sci Rep Mar 22 2016 [PMID: 27001483] (ICC/IF, Human)
• O'Hagan-Wong K, Nadeau S, Carrier-Leclerc A et al. Increased IL-6 secretion by aged human mesenchymal stromal cells disrupts hematopoietic stem and progenitor cells' homeostasis. Oncotarget 2016 Mar 22 [PMID: 26934440] (ICC/IF)
• Francia S, Cabrini M, Matti V et al. DICER, DROSHA and DNA damage-response RNAs are necessary for the secondary recruitment of DNA damage response factors J. Cell. Sci. Feb 16 2016 [PMID: 26906421] (ICC/IF, Human)
• Cekan P, Hasegawa K, Pan Y et al. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. Mol Biol Cell 2016 Apr 15 [PMID: 26864624] (WB)
• Nagy Z, Kalousi A, Furst A et al. Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs. PLoS Genet 2016 Feb [PMID: 26845027] (WB)
• Lescale C, Abramowski V, Bedora-Faure M et al. RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair. Nat Commun. 2016 Feb 02 [PMID: 26833222] (ICC/IF, Mouse)
• Martinez I, Garcia-Carpizo V, Guijarro T et al. Induction of DNA double-strand breaks and cellular senescence by human respiratory syncytial virus Virulence May 18 2016 [PMID: 26809688] (ICC/IF, Human)
• Zhang H, Liu H, Chen Y et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016 Jan 05 [PMID: 26727879] (WB, ICC/IF, Human)
• Chatalic KLS, Konijnenberg M, Nonnekens J. In Vivo Stabilization of a Gastrin-Releasing Peptide Re-ceptor Antagonist Enhances PET Imaging and Radionu-clide Therapy of Prostate Cancer in Preclinical Studies. Theranostics . 2016 Jan 01 [PMID: 26722377] (IHC-P, Mouse)
• Obermeier K, Sachsenweger J, Friedl TW et al. Heterozygous PALB2 c.1592delT mutation channels DNA double-strand break repair into error-prone pathways in breast cancer patients. Oncogene. 2015 Dec 07 [PMID: 26640152] (WB, ICC/IF, Human)
• Rall M, Kraft D, Volcic M et al. Impact of Charged Particle Exposure on Homologous DNA Double-Strand Break Repair in Human Blood-Derived Cells. Front Oncol 2015 [PMID: 26618143] (ICC/IF, Human)
• Han X, Liu Z, Jo MC et al. CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv 2015 Aug [PMID: 26601238] (ICC/IF)
• Li M, Huang R, Jiang X et al. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. PLoS ONE. 2015 Nov 25 [PMID: 26599493] (ICC/IF, Mouse)
• Werner E, Wang H, Doetsch PW. Role of Pro-inflammatory Cytokines in Radiation-Induced Genomic Instability in Human Bronchial Epithelial Cells. Radiat. Res. 2015 Nov 18 [PMID: 26579942] (ICC/IF, Human)
  
  Details:
  53BP1 antibody along with gamma-H2AX was used for the detection of DNA repair foci in irradiated Human Bronchial Epithelial Cells (HBEC3-KT) fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton™ X-100.
• Storch K, Cordes N. The impact of CDK9 on radiosensitivity, DNA damage repair and cell cycling of HNSCC cancer cells. Int. J. Oncol. 2015 Nov 13 [PMID: 26573875]
• Djuzenova CS, Zimmermann M, Katzer A et al. A prospective study on histone Y-H2AX and 53BP1 foci expression in rectal carcinoma patients: correlation with radiation therapy-induced outcome. BMC Cancer. 2015 Nov 06 [PMID: 26541290] (ICC/IF, Human)
• Markova E, Somsedikova A, Vasilyev S et al. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy. Int. J. Radiat. Biol. 2015 Nov 04 [PMID: 26537244] (ICC/IF, Human)
• Perez M, Munoz-Galvan S, Jimenez-Garcia MP et al. Efficacy of CDK4 inhibition against sarcomas depends on their levels of CDK4 and p16ink4 mRNA. Oncotarget 2015 Dec 1 [PMID: 26528855] (WB)
• Dalm SU, Nonnekens J, Doeswijk GN et al. Comparison of the Therapeutic Response to Treatment with a 177Lu-Labeled Somatostatin Receptor Agonist and Antagonist in Preclinical Models. J Nucl Med 2016 Feb [PMID: 26514177]
• Chaudhary P, Marshall TI, Currell FJ et al. Variations in the Processing of DNA Double-Strand Breaks Along 60-MeV Therapeutic Proton Beams. Int J Radiat Oncol Biol Phys 2015 Jul 29 [PMID: 26452569]
• Hehlgans S, Oppermann J, Reichert S et al. The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis. Radiat Oncol 2015 Sep 19 [PMID: 26383618] (ICC/IF, Human)
• Kubben N, Brimacombe KR, Donegan M et al. A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Methods 2015 Sep 01 [PMID: 26341717] (ICC/IF, Human)
• Weaver AN, Cooper TS, Rodriguez M et al. DNA double strand break repair defect and sensitivity to poly ADP-ribose polymerase (PARP) inhibition in human papillomavirus 16-positive head and neck squamous cell carcinoma. Oncotarget 2015 Sep 29 [PMID: 26336991] (ICC/IF)
• Chojnowski A, Ong PF, Wong ES et al. Progerin reduces LAP2alpha-telomere association in Hutchinson-Gilford progeria. Elife 2015 [PMID: 26312502]
• Lin H, Ha K, Lu G et al. Cdc14A and Cdc14B Redundantly Regulate DNA Double-Strand Break Repair. Mol Cell Biol 2015 Nov [PMID: 26283732] (Mouse)
• Durdik M, Kosik P, Gursky J et al. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and gammaH2AX foci in human lymphocytes. Cytometry A. 2015 Aug 04 [PMID: 26243567] (Flow, ICC/IF, Human)
• Campillo-Marcos I, Monte-Serrano E Lysine Methyl Transferase Inhibitors Impair H4K20me2 and 53BP1 Foci in Response to DNA Damage in Sarcomas, A Synthetic Lethality Strategy preprints Jan 1 2021
• Ortega-Martinez S. Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress. Redox Biol 2015 Aug [PMID: 26160768] (ICC/IF)
• Petroni M, Sardina F, Heil C et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ 2016 Feb [PMID: 26068589] (WB)
• Beyreuther E, Karsch L, Laschinsky L. Radiobiological response to ultra-short pulsed megavoltage electron beams of ultra-high pulse dose rate. Int. J. Radiat. Biol. 2015 May 13 [PMID: 25968557] (ICC/IF, Human)
  
  Details:
  53BP1 antibody was used for ICC-IF staining of cytospun cells of two human squamous cell carcinoma cell lines of different radiosensitivity -FaDu/undifferentiated HNSCC of moderate radiosensitivity and SKX/moderately well differentiated HNSCC of higher radiosensitivity than FaDu. Immunostaining was performed 24 h after irradiation and primary antibody was used at 1:3000 dilution with detection via AF488 conjugated secondary antibody.
• Markiewicz E, Barnard S, Haines J et al. Nonlinear ionizing radiation-induced changes in eye lens cell proliferation, cyclin D1 expression and lens shape. Open Biol. 2015 Apr 01 [PMID: 25924630] (ICC/IF, WB, Human)
  
  Details:
  53BP1 antibody was used for WB and ICC-IF applications on human lens epithgelial cell line FHL124 exposed or not to low-dose IR up to 2.28 Gy. See full text for protocol details and the data images (Figure 2 and 5).
• Yu Q, Katlinskaya YV, Carbone CJ et al. DNA-Damage-Induced Type I Interferon Promotes Senescence and Inhibits Stem Cell Function Cell Rep 2015 Apr 21 [PMID: 25921537] (ICC/IF, Human)
• Boucher D, Vu T, Bain AL et al. Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice. FASEB J. 2015 Apr 27 [PMID: 25917330] (ICC/IF, Mouse)
  
  Details:
  Vimentin antibody used for IHC on mouse cryosections from wounds that were untreated and treated with an in vitro-engineered adipose dressing (Fig 1A and 1E).
• Zong D, Callen E, Pegoraro G et al. Ectopic expression of RNF168 and 53BP1 increases mutagenic but not physiological non-homologous end joining. Nucleic Acids Res. 2015 May 26 [PMID: 25916843] (WB, ICC/IF, Mouse)
• Borel V, Boeing S, Van Wietmarschen N et al. Disrupted Control of Origin Activation Promotes Genomic Instability Upon Loss of the Pole4 and Trp53 Tumour Suppressors SSRN Electronic Journal May 7 2021
• Fukuda Takayo, Wu Wenwen, Okada Maiko et al. Class I histone deacetylase inhibitors inhibit the retention of BRCA1 and 53BP1 at the site of DNA damage. Cancer Science 2015 [PMID: 26053117] (WB, Human)
• Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015 Apr 20 [PMID: 25895060] (ICC/IF, Human)
• Yang J, Griffin P, Vera D et al. Erosion of the Epigenetic Landscape and Loss of Cellular Identity as a Cause of Aging in Mammals SSRN Journal 2019 Oct 03 (ICC/IF, Mouse)
• Augello MA, Berman-Booty LD, Carr R et al. Consequence of the tumor-associated conversion to cyclin D1b. EMBO Mol Med. 2015 Mar 18 [PMID: 25787974] (ICC/IF, Human)
• Vancevska A, Pfeiffer V, Feretzaki M, Ahmed W SMCHD1 Promotes ATM-dependent DNA Damage Signaling and Repair of Uncapped Telomeres bioRxiv (ICC/IF, Human)
• Meir M, Galanty Y, Kashani L et al. The COP9 signalosome is vital for timely repair of DNA double-strand breaks Nucleic Acids Res. 2015 Apr 08 [PMID: 25855810] (WB, Human)
• Mar FA, Debnath J, Stohr BA. Autophagy-Independent Senescence and Genome Instability Driven by Targeted Telomere Dysfunction Autophagy 2015 Mar 09 [PMID: 25751002] (ICC/IF, Human)
• Nakagawa Y, Sedukhina AS, Okamoto N et al. NF-kappaB signaling mediates acquired resistance after PARP inhibition. Oncotarget 2015 Feb 28 [PMID: 25686825]
• Surface L. Functional analysis of the histone variant H2A.Z during lineage commitment. Thesis. 2014 May 30
• Bol GM, Vesuna F, Xie M et al. Targeting DDX3 with a small molecule inhibitor for lung cancer therapy. EMBO Mol Med. 2015 Mar 27 [PMID: 25820276] (ICC/IF, Human)
• Lewinska A, Jarosz P, Czech J, Rzeszutek I. Capsaicin-induced genotoxic stress does not promote apoptosis in A549 human lung and DU145 prostate cancer cells Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2015 Feb 01 [PMID: 25813723] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used in ICC-IF staining application for the detection of 53BP1 foci on human lung A549 and DU145 prostate cancer cells which were treated or not with 5-250uM concentrations of Capsaicin for 24 hours (Fig. 5C). The immunoassay was performed on interphase nuclei and implicated - 20 minutes fixation of cells with 3.7% formaldehyde containing 0.1% Triton X-100 in PBS, blocking with 1%BSA-0.25% Triton X-100-PBS at RT for 30 minutes, incubation of cells with primary antibody at 1:200 dilution in blocking buffer for ON at 4C, detection with FITC-conjugated secondary antibody and Hoechst 33,342, analysis of data with reference to number of 53BP foci.
• Xu G, Chapman JR, Brandsma I et al. REV7 counteracts DNA double-strand break resection and affects PARP inhibition. Nature. 2015 Mar 23 [PMID: 25799992] (WB, Mouse)
• Papp SJ, Huber AL, Jordan SD et al. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. Elife. 2015 Mar 11 [PMID: 25756610] (WB, ICC/IF, IP, Human, Mouse)
• Boccardi V, Razdan N, Kaplunov J et al. Stn1 is critical for telomere maintenance and long-term viability of somatic human cells Aging Cell. 2015 Feb 14 [PMID: 25684230] (ICC/IF, Human)
• Aarts M, Bajrami I, Herrera-Abreu MT et al. Functional genetic screen identifies increased sensitivity to WEE1 inhibition in cells with defects in Fanconi Anaemia and HR pathways Mol. Cancer Ther. 2015 Feb 11 [PMID: 25673822] (ICC/IF, Human)
• Grabowska W, Kucharewicz K, Wnuk M et al. Curcumin induces senescence of primary human cells building the vasculature in a DNA damage and ATM-independent manner Age (Dordr). 2015 Feb 01 [PMID: 25649709] (ICC/IF)
  
  Details:
  53BP1 antibody used in ICC-IF application for the detection of DNA damage/DNA double-strand breaks during curcumin-induced senescence of human vascular smooth muscle cell /VSMCs and endothelial cells/Ecs. The assay involved - fixation of cells in 70 % ethanol, 1:500 dilution of primary antibody, detection of 53BP1 foci using Alexa 488 conjugated secondary antibody and DAPI (Fig 4).
• Roewenstrunk JM. RNF169 and RNF168 novel substrates of DYRK1A: connecting DYRK1A to DNA-damage repair Thesis 2018 (ICC/IF)
• Lewinska A, Wnuk M, Grabowska W et al. Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells Toxicol. Lett. 2015 Mar 18 [PMID: 25644192] (ICC/IF, Human)
• Turner R. Relationships between nuclear lamins and telomere biology in progeroid laminopathies and cancer. Thesis. 2015 (WB, ICC/IF, Human)
• Gibbs-Seymour I, Markiewicz E, Bekker-Jensen S et al. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage Aging Cell. 2015 Jan 23 [PMID: 25645366] (IP, Human)
• Torres G, Leheste JR, Ramos RL. Immunocytochemical localization of dna double-strand breaks in human and rat brain Neuroscience. 2015 Jan 27 [PMID: 25637486] (ICC/IF, Rat)
• Badie S, Carlos AR, Folio C et al. BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres EMBO J. 2015 Jan 12 [PMID: 25582120] (WB, ICC/IF, Mouse)
• Sykora P, Misiak M, Wang Y et al. DNA polymerase b deficiency leads to neurodegeneration and exacerbates Alzheimer disease phenotypes. Nucleic Acids Res 2014 Dec 30 [PMID: 25552414] (IHC-P, Mouse)
  
  Details:
  Using the DyLight 550 conjugated version of NB100-304, catalog number NB100-304R.
• Diotti R, Kalan S, Matveyenko A, Loayza D. DNA-directed Polymerase Subunits Play a Vital Role in Human Telomeric Overhang Processing. Mol. Cancer Res. 2014 Dec 17 [PMID: 25519149] (ICC/IF, Human)
• Mender I, Gryaznov S, Dikmen ZG et al. Induction of Telomere Dysfunction Mediated by the Telomerase Substrate Precursor 6-Thio-2'-Deoxyguanosine. Cancer Discov. 2014 Dec 16 [PMID: 25516420] (FISH)
• Ito H, Fujita K, Tagawa K et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. EMBO Mol Med. 2015 Jan 06 [PMID: 25510912] (IHC-P, Mouse)
• Herrera-Moyano Emilia, Moriel-Carretero Maria, Montelone Beth A, Aguilera A. The rem mutations in the ATP-binding groove of the Rad3/XPD helicase lead to Xeroderma pigmentosum-Cockayne syndrome-like phenotypes. PLoS Genet. 2014 Dec 11 [PMID: 25500814] (ICC/IF)
• Baranski OA, Kalinichenko VV, Adami GR. Increased FOXM1 expression can stimulate DNA repair in normal hepatocytes in vivo but also increases nuclear foci associated with senescence. Cell Prolif. 2014 Dec 05 [PMID: 25477198] (IHC-Fr, Mouse)
  
  Details:
  53BP1 antibody used at 1-1000 dilution for IHC-Fr application on 2% PFA fixed liver sections of C57BL/6 and Tg(TTR-FOXM1)1Rhc mice that were exposed to Diethylnitrosamine/DEN carcinogen (Figure 3 and 4).
• Uringa EJ, Baldeyron C, Odijk H et al. A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity. DNA Repair (Amst) 2015 Jan [PMID: 25463395]
• Baltanas FC, Valero J, Alonso JR et al. Nuclear Signs of Pre-neurodegeneration. Methods Mol. Biol. 2014 Nov 28 [PMID: 25431056] (ICC/IF, Mouse)
• Neumayer G, Nguyen Md. TPX2 Impacts Acetylation of Histone H4 at Lysine 16: Implications for DnA Damage Response PLoS OnE et al. 2014 Nov 04 [PMID: 25365214] (WB, ICC/IF, Human)
  
  Details:
  53BP antibody used for WB and ICC-IF on HeLa cells subjected to miRnA-mediated depletion of TPX2.
• Zimmermann M, Kibe T, Kabir S, De Lange T. TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. Genes Dev. 2014 Oct 24 [PMID: 25344324] (WB, ICC/IF, Mouse)
• Fumagalli M, Rossiello F, Mondello C, D'Adda Di Fagagna F. Stable Cellular Senescence Is Associated with Persistent DDR Activation. PLoS OnE. 2014 Oct 24 [PMID: 25340529] (ICC/IF, Human)
• Li Z, Wang H, Wang Y et al. Effect of Radiation Quality on Mutagenic Joining of Enzymatically-Induced DnA Double-Strand Breaks in Previously Irradiated Human Cells. Radiat. Res. 2014 Oct 20 [PMID: 25329962]
• Gazzaniga FS, Blackburn EH An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity Blood. 2014 Dec 10 [PMID: 25320237] (ICC/IF, KD, Human)
• Burdak-Rothkamm S, Rothkamm K, Mcclelland K et al. BRCA1, FAnCD2 and Chk1 are potential molecular targets for the modulation of a radiation-induced DnA damage response in bystander cells. Cancer Lett. 2014 Oct 07 [PMID: 25304378] (ICC/IF, Human)
• Tang M, Li Y, Zhang X et al. Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) promotes non-homologous end joining and inhibits homologous recombination repair upon DnA damage. J. Biol. Chem. 2014 Oct 07 [PMID: 25294876] (WB, ICC/IF, Human)
• Werner E, Wang H, Doetsch PW. Opposite Roles for p38MAPK-Driven Responses and Reactive Oxygen Species in the Persistence and Resolution of Radiation-Induced Genomic Instability. PLoS ONE. 2014 Oct 02 [PMID: 25271419] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF of radiation exposed HBEC-3KT cells - fixed in 4% paraformaldehyde, permeabilized with PBS-0.2% TritonX-100 (Figure 2).
• Roukos V, Burgess RC, Misteli T. Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc. 2014 Oct 01 [PMID: 25255091]
• Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014 Sep 23 [PMID: 25245017] (IHC-P, Mouse)
• Taggart LE, McMahon SJ, Currell FJ et al. The role of mitochondrial function in gold nanoparticle mediated radiosensitisation. Cancer Nanotechnol. 2014 Sep 22 [PMID: 25243023] (ICC/IF, Human)
• Pefani DE, Latusek R, Pires I et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat. Cell Biol. 2014 Sep 14 [PMID: 25218637]
• Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4. Terasawa M, Shinohara A, Shinohara M. PLoS Genet. [PMID: 25166505] (Flow, ICC/IF, Human)
• Santos J, Gil J. TRIM28/KAP1 regulates senescence. Immunol. Lett. 2014 Aug 23 [PMID: 25160591] (WB, ICC/IF, Human)
• Gole B, Baumann C, Mian E et al. Endonuclease G initiates DNA rearrangements at the MLL breakpoint cluster upon replication stress. Oncogene. 2014 Aug 18 [PMID: 25132265]
• Vassilopoulos A, Tominaga Y, Kim HS et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2014 Aug 04 [PMID: 25088202] (IHC-P, Mouse)
• Frescas David, de Lange Titia. Binding of TPP1 protein to TIN2 protein is required for POT1a,b protein-mediated telomere protection. J Biol Chem. 2014 Aug 29 [PMID: 25056954] (Mouse)
• Carlessi L, Poli EF, Bechi G et al. Functional and molecular defects of hiPSC-derived neurons from patients with ATM deficiency. Cell Death Dis 2014 Jul 18 [PMID: 25032865] (ICC/IF, Human)
  
  Details:
  53BP1 antibody used for ICC-IF on neurons derived from hiPSC /human-induced pluripotent stem cells generated from reprogrammed fibroblasts from ATM-null A-T patients and normal controls to pluripotency. Cells washed with 0.9% NaCl, air dried and fixed in 3%PFA, permeabilized in 0.5% Triton X100/PBS -5 min/4C and blocked with 5% BSA before primary labelling for staininng the nuclear foci. Figure 6e shows the IR-induced nuclear foci/53BP1 immunostaining.
• Chene G, Ouellet V, Rahimi K et al. DNA Damage Signaling and Apoptosis in Preinvasive Tubal Lesions of Ovarian Carcinoma. Int. J. Gynecol. Cancer 2014 Jul 01 [PMID: 24987917] (IHC-P, Human)
  
  Details:
  53BP1 antibody used at 1:10,000 dilution for IHC-P on a TMA containing benign fallopian tubes, serous tubal intraepithelial carcinoma (STIC), high-grade serous ovarian cancer (HGSC) from patients with STICs (associated ovarian cancer /AOC) from the same patient and HGSCs without STICs/non-AOC (FIGURE 1).
• Peng M, Xie J, Ucher A et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J. 2014 Jun 25 [PMID: 24966277] (WB, ICC/IF, Human, Mouse)
  
  Details:
  Figure 1G - WB of 53BP1 or FANCJ in U2OS cells stably expressing shRNA vectors to either control or 53BP1 (a or b) that were also transfected with siRNAs to Con or FANCJ. Figure 7 E immunofluorescence of cells with 53BP1 foci in untreated MEFs
• Naipal KA, Verkaik NS, Ameziane N et al. Functional ex vivo assay to select Homologous Recombination deficient breast tumors for PARP inhibitor treatment. Clin. Cancer Res. 2014 Jun 24 [PMID: 24963051] (IHC-P, Human)
  
  Details:
  For evaluating formation of ionizing radiation induced foci (IRIF), breast tumor tissue obtained from patients undergoing wide local excision or amputation for breast cancer were processed for IHC-P using anti-53BP1 (NB100-304) at 1/500 dilution. Figure S3 shows gamma-H2AX and 53BP1 nuclear foci indicate the induction of DSBs in tissue slices.
• Broering TJ, Alavattam KG, Sadreyev RI et al. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J. Cell Biol. 2014 Jun 09 [PMID: 24914237] (ICC/IF, Mouse)
  
  Details:
  Immunostaining of meiotic chromosome spreads (Figure 1D) and mid-pachytene spermatocytes with 53BP1 signal on XY chromatin (Figure 1E)
• Tubbs AT, Dorsett Y, Chan E et al. kAP-1 Promotes Resection of Broken DNA Ends Not Protected by Gamma-H2AX and 53BP1 in G1-Phase Lymphocytes. Mol. Cell. Biol. 2014 May 19 [PMID: 24842905] (ICC/IF, Mouse)
• Polato F, Callen E, Wong N et al. CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J. Exp. Med. 2014 May 19 [PMID: 24842372] (WB, ICC/IF, Mouse)
• Coker H, Brockdorff N. SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks. J. Cell. Sci. 2014 May 01 [PMID: 24790221] (ICC/IF, Mouse)
• Bazzi H, Anderson KV. Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc. Natl. Acad. Sci. U.S.A. 2014 Apr 15 [PMID: 24706806] (IHC-Fr, Mouse)
• Popuri V, Hsu J, Khadka P et al. Human RECQL1 participates in telomere maintenance. Nucleic Acids Res. 3/12/2014 [PMID: 24623817] (ICC/IF, Human)
• Leung JW, Agarwal P, Canny MD et al. Nucleosome Acidic Patch Promotes RNF168- and RING1B/BMI1-Dependent H2AX and H2A Ubiquitination and DNA Damage Signaling. PLoS Genet. 3/1/2014 [PMID: 24603765] (ICC/IF, Human)
• Zahn A, Eranki AK, Patenaude AM et al. Activation induced deaminase C-terminal domain links DNA breaks to end protection and repair during class switch recombination. Proc. Natl. Acad. Sci. U.S.A. 3/3/2014 [PMID: 24591601] (ChIP, Mouse)
• Porro A, Feuerhahn S et al. TERRA-reinforced association of LSD1 with MRE11 promotes processing of uncapped telomeres. Cell Rep Feb 27 2014 [PMID: 24529708] (ICC/IF, Human)
• Lamkowski Andreas, Forcheron Fabien, Agay Diane et al. DNA damage focus analysis in blood samples of minipigs reveals acute partial body irradiation. PLoS One. 2014 Feb 03 [PMID: 24498326]
• Frescas D, de Lange T. et al. TRF2-tethered TIN2 can mediate telomere protection by TPP1/POT1. Mol. Cell. Biol. 2014 Jan 28 [PMID: 24469404] (ICC/IF, Mouse)
• Frescas D, de Lange T. et al. A TIN2 dyskeratosis congenita mutation causes telomerase-independent telomere shortening in mice. Genes Dev. 2014 Jan 22 [PMID: 24449270] (ICC/IF, Mouse)
• Chen B, Gilbert LA, Cimini BA et al. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 2013 Dec 19 [PMID: 24360272] (Human)
• Mariotti LG, Pirovano G, Savage KI et al. Use of the gamma-H2AX Assay to Investigate DNA Repair Dynamics Following Multiple Radiation Exposures. PLoS One 2013 Nov 29 [PMID: 24312182] (ICC/IF, Human)
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Reviews for 53BP1 Antibody (NB100-304) (14)

Average Rating: 4.6

(Based on 14 reviews)

We have 14 reviews tested in 3 species: Human, Mouse, Human and Mouse.

reviewed by: Takahiro Ito Immunocytochemistry Human 05/24/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: IMR-90 human lung fibroblast cell line
• Species: Human
• Lot: D-6

reviewed by: Ingrid MONTARRAS Immunocytochemistry Human 08/08/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Cancer Cells
• Species: Human
• Lot: E-4

Comments

• Comments: 1/1000 dilution

reviewed by: Pauline Jeannot Immunoprecipitation Human 02/19/2019

Summary

• Application: Immunoprecipitation
• Sample Tested: human lung cancer cell line
• Species: Human
• Lot: E-6

reviewed by: Wade Baker Immunocytochemistry Mouse 02/04/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A

reviewed by: Sammy Ferri Borgogno Western Blot Mouse 11/27/2018

Summary

• Application: Western Blot
• Sample Tested: mouse epithelial pancreatic cancer cells
• Species: Mouse
• Lot: D-9 and D-10

Comments

• Comments: Membrane was blocked with 5% non-fat dry milk, 1h RT. Then incubated ON 4C with 53bp1 antibody 1:10000. After washes, membrane was incubated with HRP-conjugated secondary antibody diluted in 5% non-fat dry milk.

reviewed by: Verified Customer Immunocytochemistry Human and Mouse 08/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured cell lines
• Species: Human and Mouse
• Lot: D-6

reviewed by: Darshil Patel Immunofluorescence Mouse 07/18/2017

Summary

• Application: Immunofluorescence
• Sample Tested: MEF
• Species: Mouse

reviewed by: NIKHIL VAD Immunocytochemistry Human 06/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: A172 Glioblastoma cells
• Species: Human
• Lot: D-2-092616-AF488

Comments

• Comments: Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with 53BP1 (AF488 conjugated) antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil).

reviewed by: Grant Stewart Immunocytochemistry Human 05/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts,hela cell
• Species: Human

reviewed by: Youwei Zhang Immunocytochemistry Human 01/04/2016

Summary

• Application: Immunocytochemistry
• Species: Human
• Lot: multiple lots

reviewed by: cynthia morimoto Western Blot Human 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23755068
• Species: Human

reviewed by: Verified Customer Immunohistochemistry-Frozen Mouse 01/21/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse cochlear spiral ganglion cells
• Species: Mouse
• Lot: 3

reviewed by: Elizabeth Immunohistochemistry Mouse 07/14/2010

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse thymocytes
• Species: Mouse

reviewed by: System Admin Immunocytochemistry Mouse 05/27/2009

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse B cell
• Species: Mouse
• Lot: A

FAQs for 53BP1 Antibody (NB100-304). (Showing 1 - 4 of 4 FAQs).

1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
   • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
   • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.
3. What is the recommended pH of sodium citrate for the 53bp1 antibody (NB100-304)?
   • I have checked our notes for use of this antibody in IHC, and they say: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
4. Is possible to know the exact sequence of the epitope used?
   • Unfortunately I am unable to disclose the sequence of this antibody due to proprietary restrictions. At the site recognized by NB100-304, the reference sequences are too divergent to expect NB100-304 to bind to either Bovine or Little Brown Bat. Regarding Mouse Homology, 30/34 sites of the immunogen are homologous Human - 100% homologous. If you would be interested in testing these novel species, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: TP53BP1