HA Tag Antibody (1049F) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, C-terminal HA-tagged TRANCE, and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for HA-tagged proteins at approximately 70 kDa, 40 kDa, and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HA was detected in immersion fixed NS0 mouse myeloma cell line transfected with HA-tagged WISP-2 using Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected withC-terminal HA-tagged LRRN5 andN-terminal HA-tagged MICA, loaded at 0.1 mg/mL. Specific bands were detected for HA-tagged proteins at approximately 87 and 106 kDa (as indicated) using 1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: All-Multi
• Applications: WB, Simple Western, IP, ICC/IF
• Clone: 1049F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

HA Tag Antibody (1049F) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: HA Tag peptide
  Accession # ABB51961
• Specificity: Detects N-terminal and C-terminal HA-tagged proteins in Western blot.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 1-4 ug/100 ug cell lysate
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HA Tag Antibody (1049F) [Unconjugated]

• HA Epitope Tag
• HA Tag
• HA
• Hemagglutinin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (MAB0601)(1)

Publication using MAB0601

• Kin H. Lau, Althea N. Waldhart, Holly Dykstra, Tracey Avequin, Ning Wu PPAR gamma and C/EBP alpha response to acute cold stress in brown adipose tissue iScience 2023-01-20 [PMID: 36624847]

FAQs for HA Tag Antibody (MAB0601). (Showing 1 - 1 of 1 FAQ).

1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
   • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Bioinformatics

• Uniprot:
  • ABB51961()