LC3B Antibody - BSA Free

Images

 
Genetic Strategies: Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM CQ for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ...read more
Genetic Strategies: LC3B was detected in immersion fixed CQ treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog ...read more
Biological Strategies: Contribution of autophagy to lipid droplet formation in WT and KO NSC34 cells.(A) WT and KO NSC 34 cells were treated with (+) or without (-) bafilomycin A (bafA) under basal or HBSS ...read more
TMEM16A inhibited AngII-induced autophagy in aortas. (D) Representative immunofluorescence staining of LC3 (green) and a-SMA (red) in aortas. Scale bars, 20 um. Quantification of LC3B puncta. Five random fields (2,500 ...read more
LC3 accumulation in muscle fibers from patients with PAD (row B - E). Normal fibers from a Non-PAD sample (row A). Scale bar = 100 uM. Image from verified customer review.
Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous and extravillous trophoblast. d: decidua; v: villi; ...read more
Detection of LC3B in mouse ES cell lysates using Rabbit anti- [Catalog # NB100-2220]. LC3 detected at a molecular weight of approximately 17 kDa. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell ...read more
ORP1L and VAP-A form cholesterol-dependent ER-AV contact sites MelJuSo cells expressing GFP or GFP-tagged ORP1L mutants were fixed and stained for LC3 and Calnexin. Y477 and D478 in the FFAT motif were mutated to ...read more
Biological Strategies: Lysates of mouse NIH3T3 and rat PC-12 cell lines untreated (-) or treated (+) with CQ. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL rabbit anti-LC3B polyclonal ...read more
Biological Strategies: Detection of LC3I and LC3II in mouse cochlea cell line SV-K1 using Rabbit anti- [Catalog # NB100-2220]. Cells were treated with CQ (1 uM), and As2O3 (1 uM) for 24 hrs. Image from verified ...read more
Autophagy Investigation. (A) Representative WBs (Western Blots) of LC3B-I, LC3B-II and GAPDH loading control in DANs derived from 4 iPD patients and 4 healthy controls. (B) WBs from three independent experiments were ...read more
Biological Strategies: Expression of autophagic protein LC3 in BME-UV1 cells forming acinar structures on Matrigel. Representative images of Western blot analysis of LC3 in bovine MECs grown in 3D culture for 3, ...read more
GNA triggers the formation of autophagic markers in A549 and HeLa cell. Effects of GNA on LC3 protein. A549 cells were treated with various concentrations of GNA for 24 hours or 3 uM GNA for the indicated periods of ...read more
Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. ...read more
Autophagosomes accumulate in microglia and oligodendrocytes of the dorsal white matter adjacent to injury after SCI. Representative images of IHC staining for LC3 (green) and activated microglia marker CD11B (red) in ...read more
Staining of rat brain tissue section. Analysis using Rabbit anti-LC3B Antibody [Catalog # NB100-2220]. Image from verified customer review.
Human ovarian Cancer tissue stained using heat mediated antigen retrieval in pH 6.0 citrate buffer at 1:200 dilution. Image provided by verified customer review.
FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using 1:200 dilution of Rabbit anti-LC3B antibody [Catalog # NB100-2220]. The specific signal of LC3 was detected using HRP-conjugated secondary ...read more
Analysis using the Biotin conjugate of Rabbit anti-LC3B Antibody [Catalog # NB100-2220]. Staining of brain, cerebral cortex, neurons with cell processes.
SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD ...read more
Image shows a specific band for LC3B at a molecular weight of approximately 15 kDa in 0.5 mg/mL of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
LILRB2 antagonism effects on the bactericidal function of the MDSCs are independent of autophagy. (B) Lysates of cells from panel A were collected at 18 h and analyzed using Western blot for the autophagy marker, ...read more
CQ-induced autophagy inhibition leads to Nrf2 pathway activation in C2C12 muscle cells. a. Treatment of differentiated C2C12 myotubes with autophagy inhibitor CQ increased the sarcoplasmic level of LC3-II and SQSTM1 ...read more
Accumulation of an autophagosome marker in the presynaptic terminals of NMJs of SOD1G93A mice (A) Representative confocal micrographs of immunohistochemistry showing accumulation of the autophagosome marker LC3 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Al, Av, Ba, Bv, Ca, Ch, ChHa, Gp, Ha, In, Pm, Pm, Rb, SyHa, ZeSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, PAGE, WB, ChIP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

LC3B Antibody - BSA Free Summary

Immunogen
Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
Localization
Type I form of LC3B is cytoplasmic, whereas the type II form of LC3B binds to the autophagic membranes.
Marker
Autophagosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1LC3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Immunoprecipitation 20 ug/500 ug of protein
  • Knockdown Validated
  • Knockout Validated
  • Proximity Ligation Assay
  • SDS-Page
  • Simple Western 1:50
  • Western Blot 0.5 - 2.0 ug/mL
Application Notes
Use in SDS-PAGE reported in scientific literature (PMID:34315875).
Theoretical MW
14.688 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Cerebral Cortex Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Neuro2a Chloroquine Treated / Untreated Cell Lysate
HeLa Chloroquine Treated / Untreated Cell Lysate
Control Peptide
LC3B Protein (NB100-2220PEP)
Reviewed Applications
Read 51 Reviews rated 4.6
using
NB100-2220 in the following applications:

Publications
Read Publications using
NB100-2220 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34622072). Use in Chinese Hamster reported in scientific literature (PMID:34332287). Mouse reactivity reported in scientific literature (PMID:32814898). Bovine reactivity reported in scientific literature (PMID: 24895572). Primate reactivity reported in scientific literature (PMID: 25142602). Canine reactivity reported in scientific literature (PMID: 25839646). Avian reactivity reported in scientific literature (PMID: 29546310). Hamster reactivity reported in scientific literature (PMID: 26423766). Rabbit reactivity reported in scientific literature (PMID: 26497211). The mouse detection has been reported to be weaker than the human. Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus 84%. Invertebrate reactivity reported in scientific literature (PMID: 26716072 ). Monkey reactivity reported in scientific literature (PMID: 30324853). Guinea pig reactivity reported from a verified customer review. Rat reactivity reported in scientific literature (PMID: 30744518). Bacteria reactivity reported in scientific literature (PMID: 28783414). Chicken reactivity reported in scientific literature (PMID: 30649814). Porcine reactivity reported in scientific literature (PMID: 30789643). Golden Syrian hamster reactivity reported in scientific literature (PMID: 23180219). Zebrafish reactivity reported in scientific literature (PMID: 29185873). Human reactivity reported in scientific literature (PMID:33086573). Use in Alligator reported in scientific literature (PMID:32061056).

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LC3B Antibody - BSA Free

  • anti-LC3B
  • Apg8b
  • atg8f antibody
  • ATG8F
  • Autophagy-related protein LC3 B
  • Autophagy-related ubiquitin-like modifier LC3 B
  • lc3b autophagy marker
  • LC3B ihc
  • LC3B immunohistochemistry
  • LC3B immunoprecipitation
  • LC3B western blot
  • LC3B
  • lc3-i, ii
  • LC3II
  • MAP1 light chain 3-like protein 2
  • MAP1A/1BLC3
  • MAP1A/MAP1B LC3 B
  • MAP1LC3B
  • MAP1LC3B-a
  • microtubule associated protein 1 light chain 3 beta
  • microtubule associated protein 3 b
  • microtubule associated protein 3 beta
  • microtubule-associated proteins 1A/1B light chain 3
  • microtubule-associated proteins 1A/1B light chain 3B

Background

Autophagy (macroautophagy) is a catabolic process which targets intracellular components such as proteins and organelles for degradation. Originally described as a bulk degradation process, current research supports its selective nature (1). Selective autophagy targets specific cellular components for degradation including the endoplasmic reticulum (2) (ER-phagy), mitochondria (3) (mitophagy), peroxisomes (3) (pexophagy), ribosomes (4) (ribophagy) and bacteria (5) (xenophagy). Autophagy relies on a newly formed phagophore, a membrane structure which elongates, sequesters cellular content, and fuses to form a double membrane vesicle known as the autophagosome. Fusion of autophagosomes with lysosomes gives rise to the autophagolysosome, where cellular components are degraded by lysosome hydrolases (1).

Autophagic flux is supported by autophagy-related proteins (Atgs) initially identified in yeast (6,7). The core autophagy machinery is comprised of 17 Atg proteins that play specific roles in autophagosome formation. Among these Atg proteins, Atg8 is not only involved in autophagosome formation but also functions in cargo selection. In mammals, several Atg8 homologues have been identified including microtubule-associated protein 1 light chain 3 alpha, beta and gamma - LC3A, LC3B, and LC3C (8) respectively, as well as GABA type A receptor-associated protein (GABARAP), GABARAP-Like1, and GABARAP-Like2 (9). LC3 (predicted molecular weight 14kD) is ubiquitously expressed and undergoes posttranslational processing after synthesis. First, the cysteine protease Atg4 cleaves a carboxy terminal sequence to generate the cytosolic form LC3-I. Next, E1-like (Atg7) and E2-like (Atg3) enzymes conjugate phosphatidylethanolamine to the newly exposed carboxyterminal glycine, generating LC3-II. Finally, the Atg12-Atg5-Atg16L1 complex participates in LC3 lipidation and autophagosome formation (10). LC3B-I to LC3B-II conversion correlates with autophagosome number and is considered the best marker to monitor autophagy.

References

1. Yu, L., Chen, Y., & Tooze, S. A. (2018). Autophagy pathway: Cellular and molecular mechanisms. Autophagy. https://doi.org/10.1080/15548627.2017.1378838

2. Forrester, A., De Leonibus, C., Grumati, P., Fasana, E., Piemontese, M., Staiano, L., ... Settembre, C. (2019). A selective ER -phagy exerts procollagen quality control via a Calnexin- FAM 134B complex. The EMBO Journal. https://doi.org/10.15252/embj.201899847

3. He, X., Zhu, Y., Zhang, Y., Geng, Y., Gong, J., Geng, J., ... Zhong, H. (2019). RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradation. The EMBO Journal. https://doi.org/10.15252/embj.2018100978

4. Mathai, B., Meijer, A., & Simonsen, A. (2017). Studying Autophagy in Zebrafish. Cells. https://doi.org/10.3390/cells6030021

5. Losier, T. T., Akuma, M., McKee-Muir, O. C., LeBlond, N. D., Suk, Y., Alsaadi, R. M., ... Russell, R. C. (2019). AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles. Cell Reports. https://doi.org/10.1016/j.celrep.2019.01.062

6. Nakatogawa, H., Suzuki, K., Kamada, Y., & Ohsumi, Y. (2009). Dynamics and diversity in autophagy mechanisms: Lessons from yeast. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm2708

7. Tsukada, M., & Ohsumi, Y. (1993). Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Letters. https://doi.org/10.1016/0014-5793(93)80398-E

8. Wild, P., McEwan, D. G., & Dikic, I. (2014). The LC3 interactome at a glance. Journal of Cell Science. https://doi.org/10.1242/jcs.140426

9. Igloi, G. L. (2001). Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABAA receptor-associated protein. Genomics. https://doi.org/10.1006/geno.2001.6555

10. Glick, D., Barth, S., & Macleod, K. F. (2010). Autophagy: Cellular and molecular mechanisms. Journal of Pathology. https://doi.org/10.1002/path.2697

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2220
Species: Hu, Mu, Rt, Po, Al, Av, Ba, Bv, Ca, Ch, ChHa, Gp, Ha, In, Pm, Pm, Rb, SyHa, Ze
Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, PAGE, WB, ChIP, KD, KO

Publications for LC3B Antibody (NB100-2220)(1621)

We have publications tested in 19 confirmed species: Human, Mouse, Rat, Alligator, Avian, Bacteria, Bovine, C. elegans, Canine, Chicken, Drosophila melanogaster, Golden Syrian Hamster, Guinea Pig, Hamster, Invertebrate, Monkey, Porcine, Primate, Zebrafish.

We have publications tested in 21 applications: B/N, Chemotaxis, ELISA, EM, FLOW, Func, IB, ICC, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, In Vivo, KD, KO, PLA, SDS-Page, Simple Western, WB.


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B/N
(23)
Chemotaxis
(1)
ELISA
(2)
EM
(4)
FLOW
(11)
Func
(2)
IB
(8)
ICC
(1)
ICC/IF
(216)
IF/IHC
(65)
IHC
(15)
IHC-Fr
(15)
IHC-P
(41)
IP
(8)
In Vivo
(8)
KD
(4)
KO
(5)
PLA
(1)
SDS-Page
(1)
Simple Western
(1)
WB
(994)
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Filter By Species
Human
(625)
Mouse
(405)
Rat
(93)
Alligator
(1)
Avian
(1)
Bacteria
(3)
Bovine
(6)
C. elegans
(1)
Canine
(2)
Chicken
(6)
Drosophila melanogaster
(1)
Golden Syrian Hamster
(1)
Guinea Pig
(2)
Hamster
(1)
Invertebrate
(1)
Monkey
(3)
Porcine
(10)
Primate
(1)
Zebrafish
(9)
All Species
Showing Publications 1 - 10 of 1621. Show All 1621 Publications.
Publications using NB100-2220 Applications Species
Kopiasz ?, Dziendzikowska K, Oczkowski M et al. Low-molar-mass oat beta-glucan impacts autophagy and apoptosis in early stages of induced colorectal carcinogenesis in rats International Journal of Biological Macromolecules 2023-11-01 [PMID: 37924897] (WB, Rat) WB Rat
Lyu P Genome-wide identification of enhancers, transcription factors, and mechanisms that control skeletal muscle differentiation in cattle Thesis 2023-01-01
Kaur A, Venkatesan A, Kandarpa M et al. Lysosomal Degradation Pathways Target Mutant Calreticulin and the Thrombopoietin Receptor in Myeloproliferative Neoplasms bioRxiv 2023-07-17 (WB, Human) WB Human
Figueroa K, Gross C, Atienza E et al. GGC expansion inZFHX3causes SCA4 and impairs autophagy medRxiv 2023-10-29 (WB) WB
Kim JM, Dziobaka S, Yoon YE et al. OR2H2 Activates CAMKK?-AMPK-Autophagy Signaling Axis and Suppresses Senescence in VK2/E6E7 Cells Pharmaceuticals (Basel) 2023-08-29 [PMID: 37765029] (In Vivo, WB) In Vivo, WB
Ko SH, Choi JH, Kim JM. Bacteroides fragilis Enterotoxin Induces Autophagy through an AMPK and FoxO3-Pathway, Leading to the Inhibition of Apoptosis in Intestinal Epithelial Cells Toxins (Basel) 2023-09-03 [PMID: 37755970]
Li N, Rao L, Zhao X et al. Chlorpromazine affects autophagy in association with altered Rag GTPase-mTORC1-TFEB signaling Frontiers in cell and developmental biology 2023-09-08 [PMID: 37745295] (WB, Human) WB Human
Hattori T, Fundora KA, Hamamoto K et al. ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis Autophagy 2023-09-21 [PMID: 37733908] (WB, Human) WB Human
Bullen CK, Singh AK, Krug S et al. MDA5 RNA sensing pathway activation by Mycobacterium tuberculosis promotes innate immune subversion and pathogen survival JCI insight 2023-09-19 [PMID: 37725440] (ICC/IF, Mouse) ICC/IF Mouse
Waisner H, Lasnier S, Suma SM, Kalamvoki M Effects on exocytosis by two HSV-1 mutants unable to block autophagy Journal of virology 2023-09-15 [PMID: 37712703] (WB, Human) WB Human