SOX2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of D3 mouse embryonic stem cell line, NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was ...read more
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat SOX2 ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 53 kDa ...read more
SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room ...read more
SOX2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room ...read more
SOX2 was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
SOX2 was detected in immersion fixed paraffin-embedded sections of glioblastoma using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 3 µg/mL for 1 hour at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SOX2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Specificity
Detects human SOX2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 6 Reviews rated 4.2
using
AF2018 in the following applications:

Publications
Read Publications using
AF2018 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX2 Antibody [Unconjugated]

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Simple Western, ChIP, ICC/IF

Publications for SOX2 Antibody (AF2018)(180)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, Aedes albopictus (mosquito), Avian - Quail, Bovine, Callithrix jacchus (Marmoset), Canine, Ferret, Fish, Marmoset, Murine, Primate - Rhesus macaque, Xenopus, Zebrafish.

We have publications tested in 12 applications: ChIP, Co-Immunoprecipitation, Dot Blot, Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(13)
Co-Immunoprecipitation
(1)
Dot Blot
(1)
Flow Cytometry
(6)
ICC
(62)
ICC/IF
(2)
IF
(4)
IHC
(60)
IHC-Fr
(5)
IHC-P
(9)
Immunoprecipitation
(1)
Western Blot
(16)
All Applications
Filter By Species
Human
(92)
Mouse
(62)
Rat
(6)
Aedes albopictus (mosquito)
(1)
Avian - Quail
(1)
Bovine
(1)
Callithrix jacchus (Marmoset)
(2)
Canine
(1)
Ferret
(3)
Fish
(1)
Marmoset
(3)
Murine
(1)
Primate - Rhesus macaque
(1)
Xenopus
(4)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 180. Show All 180 Publications.
Publications using AF2018 Applications Species
M Naruse, T Saito Immediate protein expression from exogenous mRNAs in embryonic brain Scientific Reports, 2022;12(1):17145. 2022 [PMID: 36229519] (IHC, Murine) IHC Murine
Ideno H, Imaizumi K, Shimada H, Sanosaka T, Nemoto A, Kohyama J, Okano H Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms. iScience, 0;25(10):105140. 0 [PMID: 36185382] (IHC, Human) IHC Human
Y Li, Y Li, H Liu, L Cheng, H Ma, X Xu, N Zhang Generation of a human induced pluripotent stem cell line (LZUSHi002-A) from a MADD patient with ETFDH mutation Stem Cell Research, 2022;64(0):102914. 2022 [PMID: 36162333] (IHC, Human) IHC Human
AM Drozd, L Mariani, X Guo, V Goitea, NA Menezes, E Ferretti Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation International Journal of Molecular Sciences, 2022;23(18):. 2022 [PMID: 36142249] (ICC/IF, Mouse) ICC/IF Mouse
J Zhang, X Lv, B Wei, X Gong, L Chen CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer Cellular Signalling, 2022;100(0):110464. 2022 [PMID: 36075559] (Western Blot, Human) Western Blot Human
S Mukherjee, DM Luedeke, L McCoy, M Iwafuchi, AM Zorn SOX transcription factors direct TCF-independent WNT/beta-catenin responsive transcription to govern cell fate in human pluripotent stem cells Cell Reports, 2022;40(8):111247. 2022 [PMID: 36001974] (IF, Human) IF Human
W Ren, Z Ma, L Wang, X Feng, H Yu, Y Yu Lgr5+ cells are required and dynamically participate in olfactory epithelium regeneration: a revisiting shows Lgr5 expression in multiple cell lineages Theranostics, 2022;12(13):5631-5644. 2022 [PMID: 35966594] (IHC, Mouse) IHC Mouse
D Li, Q Zhang, L Li, K Chen, J Yang, D Dixit, RC Gimple, S Ci, C Lu, L Hu, J Gao, D Shan, Y Li, J Zhang, ZM Shi, D Gu, W Yuan, Q Wu, K Yang, L Zhao, Z Qiu, D Lv, W Gao, H Yang, F Lin, Q Wang, J Man, C Li, W Tao, S Agnihotri, X Qian, Y Shi, Y You, N Zhang, JN Rich, X Wang beta2-microglobulin maintains glioblastoma stem cells and induces M2-like polarization of tumor-associated macrophages Oncogene, 2022;0(0):. 2022 [PMID: 35841593] (ICC, Human) ICC Human
IB Kim, T Lee, J Lee, J Kim, S Lee, IG Koh, JH Kim, JY An, H Lee, WK Kim, YS Ju, Y Cho, SJ Yu, SA Kim, M Oh, DW Han, E Kim, JK Choi, HJ Yoo, JH Lee Non-coding de novo mutations in chromatin interactions are implicated in autism spectrum disorder Oncogene, 2022;0(0):. 2022 [PMID: 35840799] (ICC, Human) ICC Human
S Kedia, MR Aghanoori, KML Burns, M Subha, L Williams, P Wen, D Kopp, SL Erickson, EM Harvey, X Chen, M Hua, JU Perez, F Ishraque, G Yang Ubiquitination and deubiquitination of 4E-T regulate neural progenitor cell maintenance and neurogenesis by controlling P-body formation Oncogene, 2022;40(2):111070. 2022 [PMID: 35830814] (ICC, Human) ICC Human
Show All 180 Publications.

Reviews for SOX2 Antibody (AF2018) (6) 4.26

Average Rating: 4.2
(Based on 6 reviews)
We have 6 reviews tested in 4 species: Human, Mouse, Feline, Other.

Reviews using AF2018:
Filter by Applications
Immunofluorescence -free floating slices (Methanol fixation)Ideal for clearing techniques
(1)
IHC-Fr
(2)
IHC-P
(1)
IF
(1)
ChIP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Other SOX2 AF2018
Enlarge
5
reviewed by:
Bhavin Shah
Immunofluorescence -free floating slices (Methanol fixation)Ideal for clearing techniques Mouse 04/28/2020
View

Summary

ApplicationOther
Sample TestedAdult brain
SpeciesMouse
LotVRY0516091

Comments

CommentsThe samples were PFA fixed. Further treatment was in Methanol. Blocking and Staining was done with 10%NDS in PBST for primary (Overnight at 4 degrees) and secondary (RT-2hrs)
  4
reviewed by:
Kazuya Oikawa
IHC-Fr Feline 07/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedOptic nerve
SpeciesFeline
LotKOY0318041
  4
reviewed by:
Anonymous -
IHC-P Human 01/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22489004
SpeciesHuman
  4
reviewed by:
Anonymous -
IHC-Fr Mouse 01/06/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23228673
SpeciesMouse
  4
reviewed by:
Anonymous -
IF Other 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID19923274
SpeciesOther
  4
reviewed by:
Anonymous -
ChIP Human 01/06/2015
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedSee PMID 23159369
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX2 Antibody (AF2018) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Bioinformatics Tool for SOX2 Antibody (AF2018)

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Blogs on SOX2. Showing 1-10 of 12 blog posts - Show all blog posts.


  Read full blog post.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

SOX2: an Important Stem Cell Transcription Factor
SOX2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Sox-2 was found to be expressed by dividing neural progenitor cells. Constitutive expression of SOX2 has also been shown to ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.
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Recent Reviews

4.2
5
1
4
5
3
0
2
0
1
0

Bhavin Shah
04/28/2020
Application: Immunofluorescence -free floating slices (Methanol fixation)Ideal for clearing techniques
Species: Mouse

Kazuya Oikawa
07/15/2019
Application: IHC-Fr
Species: Feline

 
Anonymous -
01/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SOX2
Uniprot