SOX2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 ...read more
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat SOX2 ...read more
SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room ...read more
SOX2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 53 kDa ...read more
SOX2 was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at ...read more
SOX2 was detected in immersion fixed paraffin-embedded sections of glioblastoma using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 3 µg/mL for 1 hour at ...read more
SOX2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cells (Positive) & absent in HepG2 human hepatocellular carcinoma cells (Negative) using Goat Anti-Human/Mouse/Rat SOX2 Antigen ...read more
Knockdown OGT inhibits proliferation and tumorsphere formation of hepatoma cell through reducing eIF4E expression. A, Huh7 and PLC/PRF/5 cells were infected with control shRNA, OGT shRNA2 alone, or with wild‐type ...read more
TP63 and SOX2 staining in cervical biopsies.Immunohistochemical staining of cervical biopsies. Bars  =  50µm.(A) normal cervix no primary control; (B) normal cervix stained with anti-SOX2; (C) Squamous cell ...read more
Direct effects of glucocorticoids in ependymal glia. (A–I) Zebrafish spinal cord transverse sections. (A–C) Immunostaining of wild-types for Nr3c1 (green) and Gfap (red), plus DAPI staining (blue) with Dex treatment ...read more
Epigenetic perturbation of enhancer clustering and genome-widebinding.(A) The fluctuation range (x) and amplitude (y) were obtainedby fitting the pair-correlation function of the indicated dataset with thefluctuation ...read more
TP63 and SOX2 staining in cervical biopsies.Immunohistochemical staining of cervical biopsies. Bars  =  50µm.(A) normal cervix no primary control; (B) normal cervix stained with anti-SOX2; (C) Squamous cell ...read more
Generation of KCNA5 knockout model. (A) Illustration of polycistronic self-replicating RNA used for reprogramming (Yoshioka et al., 2013). Reading frames for OCT4, KLF4, SOX2, GLIS1, and puromycin resistance are ...read more
Construction and expression of srRNA.A: Scheme of Self-Replicating srRNA constructs. B: Expression of reprogramming factors in OKS-iM 4F-srRNA, OKS-iG 4F-srRNA, OKS-iGM 5F-srRNA, and OKS-iGML 6F-srRNA on day 2 (left) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SOX2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Specificity
Detects human SOX2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 6 Reviews rated 4.2
using
AF2018 in the following applications:

Publications
Read Publications using
AF2018 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX2 Antibody [Unconjugated]

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX2 Antibody (AF2018)(212)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Aedes albopictus (mosquito), Avian - Quail, Bovine, Callithrix jacchus (Marmoset), Canine, Chicken, Ferret, Fish, Marmoset, Primate - Rhesus macaque, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 15 applications: Binding Assay, Bioassay, ChIP, Co-Immunoprecipitation, Dot Blot, Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, RNAScope, Western Blot.


Filter By Application
Binding Assay
(1)
Bioassay
(1)
ChIP
(14)
Co-Immunoprecipitation
(2)
Dot Blot
(1)
Flow Cytometry
(9)
ICC
(81)
ICC/IF
(3)
IF
(4)
IHC
(90)
IHC-Fr
(5)
IHC-P
(11)
Immunoprecipitation
(2)
RNAScope
(1)
Western Blot
(26)
All Applications
Filter By Species
Human
(109)
Mouse
(82)
Rat
(8)
Aedes albopictus (mosquito)
(1)
Avian - Quail
(1)
Bovine
(1)
Callithrix jacchus (Marmoset)
(2)
Canine
(1)
Chicken
(2)
Ferret
(3)
Fish
(1)
Marmoset
(3)
Primate - Rhesus macaque
(1)
Transgenic Mouse
(2)
Xenopus
(5)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 212. Show All 212 Publications.
Publications using AF2018 Applications Species
Tao, Y;Li, X;Dong, Q;Kong, L;Petersen, AJ;Yan, Y;Xu, K;Zima, S;Li, Y;Schmidt, DK;Ayala, M;Mathivanan, S;Sousa, AMM;Chang, Q;Zhang, SC; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells Nature biotechnology 2023-11-16 [PMID: 37974010] (IHC, Flow Cytometry, Mouse) IHC, Flow Cytometry Mouse
Rao, SB;Brundu, F;Chen, Y;Sun, Y;Zhu, H;Shprintzen, RJ;Tomer, R;Rabadan, R;Leong, KW;Markx, S;Xu, B;Gogos, JA; Aberrant pace of cortical neuron development in brain organoids from patients with 22q11.2 deletion syndrome and schizophrenia bioRxiv : the preprint server for biology 2023-10-05 [PMID: 37873382] (Flow Cytometry, Human) Flow Cytometry Human
Zhang, Y;Tian, K;Wei, W;Mi, W;Lu, F;Liu, Z;Zhu, Q;Zhang, X;Geng, P;Qiu, J;Song, Y;Zha, D; Translocation of telomerase reverse transcriptase coincided with ATP release in postnatal cochlear supporting cells Neural regeneration research 2024-05-01 [PMID: 37862217] (ICC, Mouse) ICC Mouse
Zhu, C;Ren, X;Liu, C;Liu, Y;Wang, Y; Rbm8a regulates neurogenesis and reduces Alzheimer's disease-associated pathology in the dentate gyrus of 5×FAD mice Neural regeneration research 2024-04-01 [PMID: 37843222] (IHC, Mouse) IHC Mouse
Su, D;Eliason, S;Sun, Z;Shao, F;Amendt, BA; Wolf-Hirschhorn Syndrome Candidate 1 (Whsc1) Methyltransferase Signals Via a Pitx2-miR-23/24 Axis to Effect Tooth Development The Journal of biological chemistry 2023-10-06 [PMID: 37806494] (IHC, Mouse) IHC Mouse
She, L;Li, X;Bai, M; Generation of an induced pluripotent stem cell line XJHi001-A from a fibronectin glomerulopathy patient carrying a heterozygous NM_212482.2(C.5888-1G > C) mutation in the FN1 gene Stem cell research 2023-09-11 [PMID: 37801747] (ICC, Human) ICC Human
Rizzoti, K;Chakravarty, P;Sheridan, D;Lovell-Badge, R; SOX9-positive pituitary stem cells differ according to their position in the gland and maintenance of their progeny depends on context Science advances 2023-10-06 [PMID: 37792947] (IHC, Mouse) IHC Mouse
Jeon, HM;Shin, YJ;Lee, J;Chang, N;Woo, DH;Lee, WJ;Nguyen, D;Kang, W;Cho, HJ;Yang, H;Lee, JK;Sa, JK;Lee, Y;Kim, D;Purow, BW;Yoon, Y;Nam, DH;Lee, J; The Semaphorin 3A-Neuropilin-1 Pathway Promotes Clonogenic Growth of Glioblastoma via Activation of TGF? Signaling JCI insight 2023-10-03 [PMID: 37788099] (IHC-P, Human) IHC-P Human
Yin, J;Seo, Y;Rhim, J;Jin, X;Kim, TH;Kim, SS;Hong, JH;Gwak, HS;Yoo, H;Park, JB;Kim, JH; Crosstalk between PARN and EGFR-STAT3 Signaling Facilitates Self-Renewal and Proliferation of Glioblastoma Stem Cells Cancer research 2023-09-25 [PMID: 37747775] (Western Blot, Human) Western Blot Human
Militi, S;Nibhani, R;Jalali, M;Pauklin, S; RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Cell reports 2023-09-18 [PMID: 37725511] (ICC, Human) ICC Human
Show All 212 Publications.

Reviews for SOX2 Antibody (AF2018) (6) 4.26

Average Rating: 4.2
(Based on 6 reviews)
We have 6 reviews tested in 4 species: Human, Mouse, Feline, Other.

Reviews using AF2018:
Filter by Applications
IHC-FrFl
(1)
IHC-Fr
(2)
IHC-P
(1)
IF
(1)
ChIP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry Free-Floating SOX2 AF2018
Enlarge
5
reviewed by:
Bhavin Shah
IHC-FrFl Mouse 04/28/2020
View

Summary

ApplicationImmunohistochemistry Free-Floating
Sample TestedAdult brain
SpeciesMouse
LotVRY0516091

Comments

CommentsThe samples were PFA fixed. Further treatment was in Methanol. Blocking and Staining was done with 10%NDS in PBST for primary (Overnight at 4 degrees) and secondary (RT-2hrs)
  4
reviewed by:
Verified Customer
IHC-Fr Feline 07/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedOptic nerve
SpeciesFeline
LotKOY0318041
  4
reviewed by:
Verified Customer
IHC-P Human 01/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22489004
SpeciesHuman
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/06/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23228673
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Other 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID19923274
SpeciesOther
  4
reviewed by:
Verified Customer
ChIP Human 01/06/2015
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedSee PMID 23159369
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX2 Antibody (AF2018) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SOX2 Products

Blogs on SOX2. Showing 1-10 of 13 blog posts - Show all blog posts.

Spheroids vs. Organoids: Which 3D Cell Culture Model is Best for You?
By Jennifer Jones, M.S.Spheroids and organoids are two words that, like “butter” and “margarine”, are often referred to interchangeably but have distinct meanings. The progression and adopt...  Read full blog post.


  Read full blog post.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

SOX2: an Important Stem Cell Transcription Factor
SOX2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Sox-2 was found to be expressed by dividing neural progenitor cells. Constitutive expression of SOX2 has also been shown to ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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Recent Reviews

4.2
5
1
4
5
3
0
2
0
1
0

Bhavin Shah
04/28/2020
Application: IHC-FrFl
Species: Mouse

Verified Customer
07/15/2019
Application: IHC-Fr
Species: Feline

 
Verified Customer
01/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SOX2
Uniprot