Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Images

 
Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60Polyclonal Antibody (Catalog # AF1800) using a 1:1000 dilution ofHRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
HRP (Horseradish Peroxidase)

Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Rabbit IgG
Specificity
Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 5
using
HAF008 in the following application:

Publications
Read Publications using
HAF008 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF008)(72)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bacteria - E. Coli, Bovine, Canine, Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Primate - Marmoset.

We have publications tested in 8 applications: Bioassay, ELISA Development, Functional Assay, ICC, IHC, IHC-P, Secondary Antibody, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
Functional Assay
(1)
ICC
(1)
IHC
(4)
IHC-P
(1)
Secondary Antibody
(1)
Western Blot
(60)
All Applications
Filter By Species
Human
(31)
Mouse
(24)
Rat
(8)
Bacteria - E. Coli
(1)
Bovine
(1)
Canine
(1)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
Primate - Marmoset
(1)
All Species
Showing Publications 1 - 10 of 72. Show All 72 Publications.
Publications using HAF008 Applications Species
LJ Yu, VH Ko, DT Dao, JD Secor, A Pan, BS Cho, PD Mitchell, H Kishikawa, DR Bielenberg, M Puder Investigation of the mechanisms of VEGF-mediated compensatory lung growth: the role of the VEGF heparin-binding domain Scientific Reports, 2021;11(1):11827. 2021 [PMID: 34088930] (Western Blot, Mouse) Western Blot Mouse
MK Handzlik, DJ Tooth, D Constantin, PL Greenhaff, MA Cole Potential role for pyruvate kinase M2 in the regulation of murine cardiac glycolytic flux during in vivo chronic hypoxia Bioscience Reports, 2021;41(6):. 2021 [PMID: 33973628] (Western Blot, Mouse) Western Blot Mouse
C Drummer, EJ Vogt, M Heisterman, B Roshani, T Becker, K Mätz-Rensi, WA Kues, S Kügler, R Behr Generation and Breeding of EGFP-Transgenic Marmoset Monkeys: Cell Chimerism and Implications for Disease Modeling Cells, 2021;10(3):. 2021 [PMID: 33673402] (Western Blot, Primate - Marmoset) Western Blot Primate - Marmoset
FA Siddiqui, H Parkkola, V Vukic, C Oetken-Lin, A Jaiswal, A Kiriazis, K Pavic, T Aittokalli, TA Salminen, D Abankwa Novel Small Molecule Hsp90/Cdc37 Interface Inhibitors Indirectly Target K-Ras-Signaling Cancers, 2021;13(4):. 2021 [PMID: 33672199] (Western Blot, Human) Western Blot Human
M Tani, S Tanaka, C Oeda, Y Azumi, H Kawamura, M Sakaue, M Ito SLC37A2, a phosphorus-related molecule, increases in smooth muscle cells in the calcified aorta Journal of Clinical Biochemistry and Nutrition, 2021;68(1):23-31. 2021 [PMID: 33536709] (Western Blot, Human) Western Blot Human
P Singh, MI Reza, AA Syed, R Garg, A Husain, R Katekar, UK Goand, M Riyazuddin, AP Gupta, JR Gayen PSTi8 with metformin ameliorates perimenopause induced steatohepatitis associated ER stress by regulating SIRT-1/SREBP-1c axis Heliyon, 2020;6(12):e05826. 2020 [PMID: 33426334] (ICC, Human) ICC Human
Y Wang, Y Mi, J Tian, X Qiao, X Su, J Kang, Z Wu, G Wang, X Zhou, Y Zhou, R Li Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats Drug Des Devel Ther, 2020;14(0):4825-4834. 2020 [PMID: 33204068] (IHC, Rat) IHC Rat
CT Chao, HY Yeh, YT Tsai, TH Yuan, MT Liao, JW Huang, HW Chen Astaxanthin Counteracts Vascular Calcification In Vitro Through an Early Up-Regulation of SOD2 Based on a Transcriptomic Approach Int J Mol Sci, 2020;21(22):. 2020 [PMID: 33198315] (Western Blot, Rat) Western Blot Rat
S Ota, H Sakuraba, H Hiraga, S Yoshida, M Satake, Y Akemoto, N Tanaka, R Watanabe, M Takato, Y Murai, K Ueno, T Niioka, M Hayakari, Y Ishiguro, S Fukuda Cyclosporine protects from intestinal epithelial injury by modulating butyrate uptake via upregulation of membrane monocarboxylate transporter 1 levels Biochem Biophys Rep, 2020;24(0):100811. 2020 [PMID: 33102812] (Western Blot, Mouse) Western Blot Mouse
H Lee, JW Kim, DS Lee, SH Min Combined Poziotinib with Manidipine Treatment Suppresses Ovarian Cancer Stem-Cell Proliferation and Stemness Int J Mol Sci, 2020;21(19):. 2020 [PMID: 33036254] (Western Blot, Human) Western Blot Human
Show All 72 Publications.

Review for IgG Antibody (HAF008) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Pig.

Reviews using HAF008:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human and Pig
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western IgG HAF008
Enlarge
5
reviewed by:
Verified Customer
Simple Western Human and Pig 08/14/2019
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Summary

ApplicationSimple Western
Sample Testedhuman liver cells, Sample Amount: 15 ug,Liver tissue,Muscle tissue,Skin tissue
SpeciesHuman and Pig
LotFIN1819021

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF008). (Showing 1 - 1 of 1 FAQs).

  1. May I know the buffer used in this product?
    • It is supplied as a 0.2um filtered solution in sodium phosphate and Proclin 300

Other Available Formats

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
08/14/2019
Application: Simple Western
Species: Human and Pig

Bioinformatics

Gene Symbol IGHG1