Axl Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Axl knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431cell line, but is not detectable in knockout A431 cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Axl knockout A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins.

Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Axl Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Axl
  Glu33-Pro440
  Accession # AAA61243
• Specificity: Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AXL

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Knockout Validated
• Control:

  Axl Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl Antibody [Unconjugated]

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axl Antibody (AF154)(40)

Publications using AF154

• A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31689560] (Cell Culture, Human)
• LA Koopman, MG Terp, GG Zom, ML Janmaat, K Jacobsen, E Gresnigt-V, M Brandhorst, U Forssmann, FM de Bree, N Pencheva, A Lingnau, MA Zipeto, PW Parren, EC Breij, HJ Ditzel Enapotamab vedotin, an AXL-specific antibody-drug conjugate, shows preclinical antitumor activity in non-small cell lung cancer JCI Insight, 2019;0(0):. 2019 [PMID: 31600169] (Western Blot, Human)
• Y Xiao, H Zhao, L Tian, R Nolley, AN Diep, A Ernst, KC Fuh, YR Miao, R von Eyben, JT Leppert, JD Brooks, DM Peehl, AJ Giaccia, EB Rankin S100A10 is a critical mediator of GAS6/AXL-induced angiogenesis in renal cell carcinoma Cancer Res., 2019;0(0):. 2019 [PMID: 31585940] (IHC, Human)
• A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice Cell Rep, 2019;28(4):896-908.e5. 2019 [PMID: 31340152] (Neutralization, Human)
• DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells MBio, 2019;10(4):. 2019 [PMID: 31311882] (Neutralization, Human)
• KA Mills, JM Quinn, ST Roach, M Palisoul, M Nguyen, H Noia, L Guo, J Fazal, DG Mutch, SA Wickline, H Pan, KC Fuh p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts Sci Rep, 2019;9(1):4762. 2019 [PMID: 30886159] (Western Blot, Human)
• L Sinik, KA Minson, JJ Tentler, J Carrico, SM Bagby, WA Robinson, R Kami, T Burstyn-Co, SG Eckhardt, X Wang, SV Frye, HS Earp, D DeRyckere, DK Graham Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma Mol. Cancer Ther., 2019;18(2):278-288. 2019 [PMID: 30482852] (Western Blot, Human)
• Q Guo, T Grimmig, G Gonzalez, A Giobbie-Hu, G Berg, N Carr, BJ Wilson, P Banerjee, J Ma, JS Gold, B Nandi, Q Huang, AM Waaga-Gass, CG Lian, GF Murphy, MH Frank, M Gasser, NY Frank ATP-binding cassette member B5 (ABCB5) promotes tumor cell invasiveness in human colorectal cancer J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29789423] (Western Blot, Human)
• A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman Human Sertoli cells support high levels of Zika virus replication and persistence Sci Rep, 2018;8(1):5477. 2018 [PMID: 29615760] (Neutralization, Human)
• M Persaud, A Martinez-L, C Buffone, SA Porcelli, F Diaz-Griff Infection by Zika viruses requires the transmembrane protein AXL, endocytosis and low pH Virology, 2018;518(0):301-312. 2018 [PMID: 29574335] (Flow Cytometry, Human)
• BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono Protein S and Gas6 induce efferocytosis of HIV-1-infected cells Virology, 2018;515(0):176-190. 2018 [PMID: 29304470] (Neutralization, Human)
• NB Leighl, MS Tsao, G Liu, D Tu, C Ho, FA Shepherd, N Murray, JR Goffin, G Nicholas, S Sakashita, Z Chen, L Kim, J Powers, L Seymour, G Goss, PA Bradbury A phase I study of foretinib plus erlotinib in patients with previously treated advanced non-small cell lung cancer: Canadian cancer trials group IND.196 Oncotarget, 2017;8(41):69651-69662. 2017 [PMID: 29050231] (IHC, Human)
• C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma Br. J. Cancer, 2017;117(12):1787-1797. 2017 [PMID: 29024938] (Flow Cytometry, Human)
• SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation J. Immunol., 2017;0(0):. 2017 [PMID: 28916522] (Western Blot, Human)
• R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy Sci Rep, 2017;7(1):10613. 2017 [PMID: 28878389] (Western Blot, Human)
• AK Hastings, LJ Yockey, BW Jagger, J Hwang, R Uraki, HF Gaitsch, LA Parnell, B Cao, IU Mysorekar, CV Rothlin, E Fikrig, MS Diamond, A Iwasaki TAM Receptors Are Not Required for Zika Virus Infection in Mice Cell Rep, 2017;19(3):558-568. 2017 [PMID: 28423319] (Western Blot, Human)
• AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):2024-2029. 2017 [PMID: 28167751] (Neutralization, Human)
• KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication Sci Rep, 2017;7(0):41389. 2017 [PMID: 28128342] (ICC, Human)
• Implication of the Receptor Tyrosine Kinase AXL in Head and Neck Cancer Progression Int J Mol Sci, 2016;18(1):. 2016 [PMID: 28025482] (IHC, Human)
• Zhi-Min Yuan AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex J Mol Cell Biol, 2016;0(0):. 2016 [PMID: 27927748] (Western Blot, Human)
• Zika virus cell tropism in the developing human brain and inhibition by azithromycin Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):14408-14413. 2016 [PMID: 27911847] (Neutralization, Human)
• Mihalis S Kariolis Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies J. Clin. Invest, 2016;0(0):. 2016 [PMID: 27893463] (Western Blot, Mouse)
• LM Divine, MR Nguyen, E Meller, RA Desai, B Arif, EB Rankin, KH Bligard, C Meyerson, IS Hagemann, M Massad, PH Thaker, AR Hagemann, CK McCourt, MA Powell, DG Mutch, KC Fuh AXL modulates extracellular matrix protein expression and is essential for invasion and metastasis in endometrial cancer Oncotarget, 2016;7(47):77291-77305. 2016 [PMID: 27764792] (IHC, Human)
• ZEB1-mediated melanoma cell plasticity enhances resistance to MAPK inhibitors EMBO Mol Med, 2016;0(0):. 2016 [PMID: 27596438] (Western Blot, Human)
• Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers J Clin Invest, 2016;0(0):. 2016 [PMID: 27159396] (Western Blot, Human)
• K Sato, K Suda, S Shimizu, K Sakai, H Mizuuchi, K Tomizawa, T Takemoto, K Nishio, T Mitsudomi Clinical, Pathological, and Molecular Features of Lung Adenocarcinomas with AXL Expression PLoS ONE, 2016;11(4):e0154186. 2016 [PMID: 27100677] (IHC-P, Human)
• KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles J. Biol. Chem., 2016;291(20):10586-601. 2016 [PMID: 27006397] (Western Blot, Human)
• Fritz H, Gustafsson A, Ljungberg B, Ceder Y, Axelson H, Dahlback B The Axl-Regulating Tumor Suppressor miR-34a Is Increased in ccRCC but Does Not Correlate with Axl mRNA or Axl Protein Levels. PLoS ONE, 2015;10(8):e0135991. 2015 [PMID: 26287733] (Flow Cytometry, Human)
• Ji, Wonjun, Choi, Chang-Mi, Rho, Jin Kyun, Jang, Se Jin, Park, Young So, Chun, Sung-Min, Kim, Woo Sung, Lee, Jung-Shi, Kim, Sang-We, Lee, Dae Ho, Lee, Jae Cheo Mechanisms of acquired resistance to EGFR-tyrosine kinase inhibitor in Korean patients with lung cancer. BMC Cancer, 2013;13(0):606. 2013 [PMID: 24369725] (IHC-P, Human)
• Rho, Jin Kyun, Choi, Yun Jung, Kim, Seon Ye, Kim, Tae Won, Choi, Eun Kyun, Yoon, Seon-Joo, Park, Bu Man, Park, Eunhye, Bae, Jong Hwa, Choi, Chang-Mi, Lee, Jae Cheo MET and AXL inhibitor NPS-1034 exerts efficacy against lung cancer cells resistant to EGFR kinase inhibitors because of MET or AXL activation. Cancer Res, 2014;74(1):253-62. 2014 [PMID: 24165158] (Western Blot, Human)
• Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. 2012 [PMID: 23071254] (Neutralization, Human)
• Zhang Z, Lee JC Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Nat. Genet., 2012;44(8):852-860. 2012 [PMID: 22751098] (Immunoprecipitation, Human)
• Brown JE, Krodel M, Pazos M Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells. PLoS ONE, 2012;7(5):e36800. 2012 [PMID: 22606290] (Immunoprecipitation, Human)
• Migdall-Wilson J, Bates C, Schlegel J, Brandao L, Linger RM, Deryckere D, Graham DK Prolonged exposure to a Mer ligand in leukemia: Gas6 favors expression of a partial Mer glycoform and reveals a novel role for Mer in the nucleus. PLoS ONE, 2012;7(2):e31635. 2012 [PMID: 22363695] (Western Blot, Human)
• Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. J. Virol., 2012;86(4):2067-78. 2012 [PMID: 22156524] (Flow Cytometry, Human)
• Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Thromb. Haemost., 2011;107(1):140-9. 2011 [PMID: 22072012] (ICC, Human)
• Butler GS, Dean RA, Tam EM, Overall CM Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Mol. Cell. Biol., 2008;28(15):4896-914. 2008 [PMID: 18505826] (Western Blot, Human)
• Uehara H, Shacter E Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. J. Immunol., 2008;180(4):2522-30. 2008 [PMID: 18250462] (Western Blot, Human)
• Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP, Canfield AE Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells. Circ. Res., 2007;100(4):502-9. 2007 [PMID: 17255529] (Immunoprecipitation, Bovine)
• Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. J. Thromb. Haemost., 2005;3(4):733-41. 2005 [PMID: 15733062] (Flow Cytometry, Human)

Reviews for Axl Antibody (AF154) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous IHC-Fr Human 01/05/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23071254
• Species: Human

reviewed by: Anonymous WB Human 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 20103767
• Species: Human

Blocking

• Blocking Details: See PMID 20103767

Details

• Detection Notes: See PMID 20103767

Comments

• Comments: See PMID 20103767

Bioinformatics

• Gene Symbol: AXL
• Uniprot:
  • AAA61243()