ERK2 Antibody (4C11) - BSA Free

Images

 
Western Blot: ERK2 Antibody (4C11) [NB110-60506] - Analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.
Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (4C11) [NB110-60506] - Analysis of Eca-109 cells using ERK2 mouse mAb (green).
Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) [NB110-60506] - Analysis of human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 mouse mAb with DAB staining.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
4C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price

ERK2 Antibody (4C11) - BSA Free Summary

Immunogen
Purified recombinant fragment of human ERK2 expressed in E. Coli.
Specificity
ERK2 (4C11C11C4)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAPK1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ERK2 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for ERK2 Antibody (4C11) - BSA Free

  • EC 2.7.11
  • EC 2.7.11.24
  • ERK
  • ERK2
  • ERK-2
  • ERK2MAP kinase isoform p42
  • ERT1
  • Extracellular signal-regulated kinase 2
  • MAP kinase 1
  • MAP kinase 2
  • MAPK 1
  • MAPK1
  • MAPK2
  • mitogen-activated protein kinase 1
  • Mitogen-activated protein kinase 2
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • p42-MAPK
  • PRKM1
  • PRKM1MAPK 2
  • PRKM2
  • protein tyrosine kinase ERK2

Background

ERK2 (also designated extracellular-signal-related kinase 2 or mitogen-activated protein kinase 1), with 360-amino acid protein (about 40kDa), belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF1347
Species: Hu, Mu, Rt
Applications: ICC, IHC, Simple Western, WB
NBP2-67514
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-67471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
210-TA/CF
Species: Hu
Applications: BA
NBP2-67358
Species: Bv, Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
H00005706-M02
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, KD, WB
NBP1-89065
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
D6050
Species: Hu
Applications: ELISA
NBP2-29323
Species: Bv, Ca, Ch, Hu, Mu, Rt, Xp
Applications: Func-Inh
MAB4540
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NB110-60506
Species: Hu, Mu, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for ERK2 Antibody (NB110-60506) (0)

There are no publications for ERK2 Antibody (NB110-60506).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ERK2 Antibody (NB110-60506) (0)

There are no reviews for ERK2 Antibody (NB110-60506). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERK2 Antibody (NB110-60506) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ERK2 Products

Bioinformatics Tool for ERK2 Antibody (NB110-60506)

Discover related pathways, diseases and genes to ERK2 Antibody (NB110-60506). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ERK2 Antibody (NB110-60506)

Discover more about diseases related to ERK2 Antibody (NB110-60506).
 

Pathways for ERK2 Antibody (NB110-60506)

View related products by pathway.

PTMs for ERK2 Antibody (NB110-60506)

Learn more about PTMs related to ERK2 Antibody (NB110-60506).
 

Research Areas for ERK2 Antibody (NB110-60506)

Find related products by research area.

Blogs on ERK2.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. Enc...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ERK2 Antibody (4C11) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol MAPK1
Entrez