HIF-1 alpha Antibody - BSA Free

Chromatin Immunoprecipitation: HIF-1 alpha Antibody [NB100-479] - ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Each result represents at least 3 independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (*p<0.05, **p<0.01). Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY licence.

Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Immunofluorescence of wounded tissue and normal unwounded skin. Representative images for immunofluorescent staining of HIF-1 alpha on healed wounded skin and adjacent normal unwounded skin of wild type, heterozygous K14-Cre/homozygous floxed PHD-2, and heterozygous Col1 alpha2-Cre-ER/homozygous floxed PHD-2 mice at 400x magnification. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0093373), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis of HIF-1 alpha in mouse hepatocytes (left lane: normoxic and right lane: hypoxic) using HIF-1 alpha antibody. Western blot image submitted by a verified customer review.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in paraffin section of mouse skin wound using NB100-479 at 1:6000. Image courtesy of product review by Ian Darby of RMIT University.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-479] - Immunofluorescence (IF) labeling and confocal microscopy analysis of the distribution of HIF-1alpha (A1-D1) and Abeta (A2-D2) in APPsw cells. DFO treatment significantly increased HIF-1alpha IF (D1) and reduced the Abeta IF (D2) in APPsw cells compared with controls (A1,A2). (A3-D3) DAPI was used to detect the nucleus (blue). (A4-D4) Merged images from the double channels indicated that DFO has an inhibitory effect on Abeta formation, and is involved in HIF-1alpha stabilization in APPsw cells. Fe treatment (100 uM) decreased HIF-1alpha (B1) and increased the Abeta levels (B2). DFO treatment reversed the reduction of HIF-1alpha (C1) and the increase of Abeta (C2) in cultures pretreated with 100 uM Fe. Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnagi.2015.00104/abstract) licensed under a CC-BY licence.

Chromatin Immunoprecipitation: HIF-1 alpha Antibody [NB100-479] - MDA-MB-231 cells were exposed to 20% or 1% O2 for 16 hours, and chromatin immunoprecipitation (ChIP) was performed with the indicated antibody (Ab). Primers flanking the HIF binding site in the S100A10 gene were used for qPCR. ChIP image submitted by a verified customer review.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.

Western Blot: HIF-1 alpha Antibody [NB100-479] - U251 cells exposed to 4 hour hypoxia (0.5% oxygen) incubation. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-479] - Detection of HIF-1 Alpha (green) in RCC4 cells using NB100-479. Nuclei (Blue) were counterstained using Hoechst 33258.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis of HIF-1 alpha in human kidney tumor.

Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Staining of human placenta, villi.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Effects of different routes of CDDP administration on remaining viable tumor tissue. Representative images of tumor tissue (i.v. CDDP, i.a. CDDP) stained for HIF-1alpha (20X). Scale bar corresponds to 100 um. Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-016-0392-1), licensed under a CC-BY licence.

Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Hif1alpha expression in preimplantation embryos. Co-staining of OCT4 and Hif1alpha in inner cell mass of blastocyst (top) and negative control images without primary antibodies (bottom). Scale bar: 30um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY licence.

Simple Western: HIF-1 alpha Antibody [NB100-479] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NB100-479 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard seen with this antibody. Image from an internal validation.

• Reactivity: Hu, Mu, Rt, Po, Ca, Fi, Gt, Ha, Pm, Rb, Ze
• Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-WhMt
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HIF-1 alpha Antibody - BSA Free Summary

• Immunogen: This HIF-1 alpha antibody was developed against a fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Frozen 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western
  • Western Blot 1-2ug/ml
• Application Notes: WB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 27148974).
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
  COS-7 Nuclear Hypoxic Induced Cell Lysate
  Human Bone Marrow Whole Tissue Lysate (Adult Whole Diabetes)
  HeLa CoCl2 treated/Untreated Cell Lysate
  HepG2 CoCl2 treated/Untreated Cell Lysate
  HeLa Hypoxic / Normoxic Cell Lysate
  HepG2 Hypoxic / Normoxic Cell Lysate
  HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
  HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
  NIH 3T3 CoCl2 treated/Untreated Cell Lysate
  L929 CoCl2 treated/Untreated Cell Lysate
  Raw 264.7 CoCl2 treated/Untreated Cell Lysate
  PC12 CoCl2 treated/Untreated Cell Lysate
  PC12 Hypoxic / Normoxic Cell Lysate
  Raw 264.7 Hypoxic / Normoxic Cell Lysate
  L929 Hypoxic / Normoxic Cell Lysate
  NIH 3T3 Hypoxic / Normoxic Cell Lysate

• Control Peptide:

  HIF-1 alpha Recombinant Protein (NBC1-18422)

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:35457018).Use in Mouse reported in scientific literature (PMID:33727588). Use in Goat reported in scientific literature (PMID:21599540).

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HIF-1 alpha Antibody - BSA Free

• AINT
• anti-HIF-1 alpha
• anti-HIF1A
• ARNT interacting protein
• ARNT-interacting protein
• Basic-helix-loop-helix-PAS protein MOP1
• BHLHE78
• Class E basic helix-loop-helix protein 78
• HIF 1A
• HIF1 alpha
• HIF-1 alpha
• HIF1
• HIF1A
• HIF-1a
• HIF-1alpha
• HIF-1-alpha
• HIF1-alpha
• hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha
• hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• hypoxia-inducible factor 1-alpha
• Member of PAS protein 1
• member of PAS superfamily 1
• MOP1
• PAS domain-containing protein 8
• PASD8

Background

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).

Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205

6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016

7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIF-1 alpha Antibody (NB100-479)(447)

Publications using NB100-479

• Maki T, Sawahata M, Akutsu I et al. APP Knock-In Mice Produce E22P-AB Exhibiting an Alzheimer’s Disease-like Phenotype with Dysregulation of Hypoxia-Inducible Factor Expression International Journal of Molecular Sciences Oct 31 2022 [PMID: 36362046] (WB, Mouse)
• Qian L, Rawashdeh O, Kasas L et al. Cholinergic basal forebrain degeneration due to sleep-disordered breathing exacerbates pathology in a mouse model of Alzheimer's disease Nature communications Nov 2 2022 [PMID: 36323689] (IHC-FrFl, Mouse)
• Chang YH, Vuong CK, Ngo NH et al. Extracellular vesicles derived from Wharton's Jelly mesenchymal stem cells inhibit the tumor environment via the miR-125b/HIF1 alpha signaling pathway Scientific reports Aug 8 2022 [PMID: 35941273] (WB, Human)
• Yang Y, Chen C, Suo Q et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv Dec 9 2022 [PMID: 36490339] (ChIP, Human)
• Yin L, Zhang J, Sun Y Early growth response-1 is a new substrate of the GSK3beta-FBXW7 axis Neoplasia (New York, N.Y.) Oct 11 2022 [PMID: 36240645]
• Soler-Agesta R, Marco-Brualla J, MinjArez-SAenz M et al. PT-112 Induces Mitochondrial Stress and Immunogenic Cell Death, Targeting Tumor Cells with Mitochondrial Deficiencies Cancers Aug 9 2022 [PMID: 36010843] (WB, Mouse)
• Simmler P, Cortijo C, Koch LM et al. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer Cell reports Aug 23 2022 [PMID: 36001976] (ChIP, WB)
• Chen J, Tang TT, Cao JY et al. KIM-1 augments hypoxia-induced tubulointerstitial inflammation through uptake of small extracellular vesicles by tubular epithelial cells Molecular therapy : the journal of the American Society of Gene Therapy Aug 18 2022 [PMID: 35982620] (IHC-P, Mouse)
• Wang H, Wang X, Ma L et al. PGC-1 alpha regulates mitochondrial biogenesis to ameliorate hypoxia-inhibited cementoblast mineralization Annals of the New York Academy of Sciences Aug 2 2022 [PMID: 35917205]
• Takiyama T, Sera T, Nakamura M et al. A maternal high-fat diet induces fetal origins of NASH-HCC in mice Scientific reports Jul 30 2022 [PMID: 35907977] (IHC-P, Mouse)
• Li G, Li D, Wu C et al. Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9 Experimental & molecular medicine Jul 20 2022 [PMID: 35859119] (IP, Human)
• Liu Y, Gu Y, Ng S et al. Circulating levels of hydroxylated bradykinin function as an indicator of tissue HIF-1alpha expression Science Bulletin Jan 1 2020 (IHC-P, Human)
• Burda JE, O'Shea TM, Ao Y et al. Divergent transcriptional regulation of astrocyte reactivity across disorders Nature May 25 2022 [PMID: 35614216] (IHC-Fr, Mouse)
• Menendez-Montes I, Escobar B, Gomez MJ Activation of amino acid metabolic program in response to impaired glycolysis in cardiac HIF1 deficient mice bioRxiv Jan 1 2020 (IHC, Mouse)
• Ollonen T, Kurkela M, Laitakari A et al. Activation of the hypoxia response protects mice from amyloid-beta accumulation Cellular and molecular life sciences : CMLS Jul 19 2022 [PMID: 35852609] (WB, Mouse)
  
  Details:
  1:500 Dilution
• Tapio J, Halmetoja R, Dimova E et al. Contribution of HIF-P4H isoenzyme inhibition to metabolism indicates major beneficial effects being conveyed by HIF-P4H-2 antagonism Journal of Biological Chemistry Jul 1 2022 [PMID: 35787374] (WB, Mouse)
• Chen M, Hu R, Cavinato C et al. Fibronectin-integrin alpha 5 signaling in vascular complications of type 1 diabetes Diabetes Jun 30 2022 [PMID: 35771994]
• Li S, Li W, Yuan J et al. Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome Nature metabolism Jun 1 2022 [PMID: 35760869] (WB, Human)
• Fu Y, Cen D, Zhang T et al. Implantable fibrous scaffold with hierarchical microstructure for the ‘on-site’ synergistic cancer therapy Chemical Engineering Journal Dec 1 2020 (WB, Mouse)
• Zhang RM, Tiedemann K, Muthu ML et al. Fibrillin-1-regulated miR-122 has a critical role in thoracic aortic aneurysm formation Cellular and molecular life sciences : CMLS May 23 2022 [PMID: 35606547]
• Baran N, Lodi A, Dhungana Y et al. Inhibition of mitochondrial complex I reverses NOTCH1-driven metabolic reprogramming in T-cell acute lymphoblastic leukemia Nature communications May 19 2022 [PMID: 35589701] (CyTOF, Human)
• Salman S, Meyers D, Wicks E et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 44597 [PMID: 35499076] (WB, ChIP, Human, Mouse)
• Leigh T, Kawai T, Preston K et al. Deletion of LDLRAP1 Induces Atherosclerotic Plaque Formation, Insulin Resistance, and Dysregulated Insulin Response in Adipose Tissue The American journal of pathology Apr 20 2022 [PMID: 35460615] (IHC, Mouse)
• Wu X, Chen J, Liu C et al. Slc38a9 Deficiency Induces Apoptosis and Metabolic Dysregulation and Leads to Premature Death in Zebrafish International journal of molecular sciences Apr 11 2022 [PMID: 35457018] (IP, Zebrafish)
• Xu ZH, Wang C, He YX et al. Hypoxia-inducible factor protects against acute kidney injury via the Wnt/beta-catenin signaling pathway American journal of physiology. Renal physiology [PMID: 35403451]
• Kirschner K, Kelterborn S, Stehr H et al. Adaptation of the Oxygen Sensing System during Lung Development Oxidative Medicine and Cellular Longevity Feb 18 2022 [PMID: 35242281] (WB, Mouse)
• Jatho A, Zieseniss A, Brechtel-Curth K et al. The HIF alpha-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells Cells Feb 21 2022 [PMID: 35203399] (WB)
• Snyder B, Wu HK, Tillman B, Floyd TF Aged Mouse Hippocampus Exhibits Signs of Chronic Hypoxia and an Impaired HIF-Controlled Response to Acute Hypoxic Exposures Cells Jan 26 2022 [PMID: 35159233] (IHC-Fr, Mouse)
• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications Jan 14 2022 [PMID: 35031618] (ChIP)
• Elamaa H, Kaakinen M, NAtynki M et al. PHD2 deletion in endothelial or arterial smooth muscle cells reveals vascular cell type-specific responses in pulmonary hypertension and fibrosis Angiogenesis Jan 8 2022 [PMID: 34997404] (WB, Mouse)
• Michon S, Rodier F, Yu FTH Targeted Anti-Cancer Provascular Therapy Using Ultrasound, Microbubbles, and Nitrite to Increase Radiotherapy Efficacy Bioconjugate chemistry Jan 6 2022 [PMID: 34990112]
• ROning T, Magga J, Laitakari A et al. Activation of the hypoxia response pathway protects against age-induced cardiac hypertrophy Journal of molecular and cellular cardiology Dec 14 2021 [PMID: 34919895] (WB, Mouse)
• Te Boekhorst V, Jiang L, MAhlen M et al. Calpain-2 regulates hypoxia/HIF-induced plasticity toward amoeboid cancer cell migration and metastasis Current biology : CB Dec 6 2021 [PMID: 34883047]
• Borella R, De Biasi S, Paolini A et al. Metabolic reprograming shapes neutrophil functions in severe COVID-19 European journal of immunology Dec 6 2021 [PMID: 34870329] (WB, Human)
• Suwa T, Kobayashi M, Shirai Y Et al. SPINK1 as a plasma marker for tumor hypoxia and a therapeutic target for radiosensitization JCI insight Nov 8 2021 [PMID: 34747365] (IHC-P, Human)
• Wang J, Zhang H, Kaul A Et al. Effect of Ashwagandha Withanolides on Muscle Cell Differentiation Biomolecules Oct 4 2021 [PMID: 34680087] (WB, ICC/IF, Mouse)
• Foglia B, Sutti S, Cannito S Et al. Hepatocyte-Specific Deletion of HIF2 alpha Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation Cellular and molecular gastroenterology and hepatology Oct 14 2021 [PMID: 34655812]
• Pang L, Ng KT, Liu J et al. Plasmacytoid dendritic cells recruited by HIF-1 alpha /eADO/ADORA1 signaling induce immunosuppression in hepatocellular carcinoma Cancer letters Sep 16 2021 [PMID: 34536555] (ChIP, Human)
• Jeny F, Bernaudin JF, Valeyre D Et al. Hypoxia Promotes a Mixed Inflammatory-Fibrotic Macrophages Phenotype in Active Sarcoidosis Frontiers in immunology Aug 11 2021 [PMID: 34456926] (ICC/IF, Human)
• Helena J, Joubert A, Mabeta P Et al. Intracellular Signaling Responses Induced by Radiation within an In Vitro Bone Metastasis Model after Pre-Treatment with an Estrone Analogue Cells Aug 17 2021 [PMID: 34440874] (WB, Human, Mouse)
• Wang J, Liu R, Wang Y Et Al. Repression of the miR-627-5p by histone deacetylase 3 contributes to hypoxia-induced hepatocellular carcinoma progression Journal of Cancer Jul 27 2021 [PMID: 34335948] (ChIP)
• Nanduri J, Wang N, Wang Bl, Prabhakar Nr Lysine demethylase KDM6B regulates HIF-1 alpha mediated systemic and cellular responses to intermittent hypoxia Physiological genomics Jul 23 2021 [PMID: 34297635]
• Ju S, Lim L, Wi K et al. LRP5 Regulates HIF-1 alpha Stability via Interaction with PHD2 in Ischemic Myocardium International Journal of Molecular Sciences Jun 19 2021 [PMID: 34205318] (IP, Rat)
• Williams AL, Walton CB, Pinell B et al. Ischemic heart injury leads to HIF1-dependent differential splicing of CaMK2 gamma Scientific reports Jun 23 2021 [PMID: 34162925] (Mouse)
• Simmen S, Maane M, Rogler S et al. Hypoxia Reduces the Transcription of Fibrotic Markers in the Intestinal Mucosa Inflammatory Intestinal Diseases Mar 29 2021 [PMID: 34124180]
• Wang N, Peng Y, Su X et al. Histone Deacetylase 5 Is an Early Epigenetic Regulator of Intermittent Hypoxia Induced Sympathetic Nerve Activation and Blood Pressure Frontiers in Physiology May 17 2021 [PMID: 34079475] (WB, Mouse)
• Grubman A, Choo XY, Chew G et al. Transcriptional signature in microglia associated with A beta plaque phagocytosis Nature communications May 21 2021 [PMID: 34021136] (IHC, Mouse)
• Bae WY, Choi JS, Nam S, Jeong JW beta-arrestin 2 stimulates degradation of HIF-1 alpha and modulates tumor progression of glioblastoma Cell death and differentiation May 18 2021 [PMID: 34007068]
• Silwal P, Kim JK, Jeon SM et al. Mitofusin-2 boosts innate immunity through the maintenance of aerobic glycolysis and activation of xenophagy in mice Communications biology May 10 2021 [PMID: 33972668]
• Wang R, Godet I, Yang Y, et al. Hypoxia-inducible factor-dependent ADAM12 expression mediates breast cancer invasion and metastasis Proc Natl Acad Sci 2021 May 11 [PMID: 33952697] (WB, ChIP)
• Pantazopoulou V, Jeannot P, Rosberg R et al. Hypoxia-Induced Reactivity of Tumor-Associated Astrocytes Affects Glioma Cell Properties Cells Mar 10 2021 [PMID: 33802060] (WB)
• Yano, M, Miyazawa, M Et al. Up-regulation of HDAC6 Results in Poor Prognosis and Chemoresistance in Patients With Advanced Ovarian High-grade Serous Carcinoma. Anticancer Res Mar 1 2021 [PMID: 33788761]
• Hutami IR, Izawa T, Khurel-Ochir T et al. HIF-1 alpha controls palatal wound healing by regulating macrophage motility via S1P/S1P1 signaling axis Oral diseases Mar 23 2021 [PMID: 33759275]
• Khuu MA, Nallamothu T, Castro-Rivera CI et al. Stage-dependent effects of intermittent hypoxia influence the outcome of hippocampal adult neurogenesis Scientific reports Mar 16 2021 [PMID: 33727588] (IHC-Fr, Mouse)
• Akyuz E, Doganyigit Z, Eroglu E et al. Myocardial Iron Overload in an Experimental Model of Sudden Unexpected Death in Epilepsy Frontiers in Neurology Feb 11 2021 [PMID: 33643194] (IHC-P, Rat)
• Plastino F, Santana-Garrido A, Pesce NA et al. Echinomycin mitigates ocular angiogenesis by transcriptional inhibition of the hypoxia-inducible factor-1 Experimental eye research Feb 24 2021 [PMID: 33639134] (WB, Mouse)
• Tanimura A, Nakazato A, Tanaka N MYD88 signals induce tumour-initiating cell generation through the NF-kappa B-HIF-1 alpha activation cascade Scientific reports Feb 17 2021 [PMID: 33597599] (WB, Mouse)
• Pesce NA, Canovai A, Lardner E, et al. Autophagy Involvement in the Postnatal Development of the Rat Retina Cells Jan 17 2021 [PMID: 33477313] (WB, Rat)
• Goggins BJ, Minahan K, Sherwin S, et al. Pharmacological HIF-1 stabilization promotes intestinal epithelial healing through regulation of alpha -integrin expression and function American journal of physiology. Gastrointestinal and liver physiology Jan 20 2021 [PMID: 33470153] (ChIP, Human)
• Markolin P, Davidson N, Hirt CK, et al. Identification of HIF-dependent alternative splicing in gastrointestinal cancers and characterization of a long, coding isoform of SLC35A3 Genomics Jan 5 2021 [PMID: 33418078] (WB, Human)
• Tran CW, Gold MJ, Garcia-Batres C, et al. Hypoxia-inducible factor 1 alpha limits dendritic cell stimulation of CD8 T cell immunity PloS one Dec 31 2020 [PMID: 33382742] (WB, Mouse)
• Pelaz SG, JaraIz-RodrIguez M, Alvarez-VAzquez A et al. Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43266-283 EBioMedicine Nov 27 2020 [PMID: 33254027] (WB)
• Almeida L, Dhillon-LaBrooy A, Castro CN et al. Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis Immunity Nov 17 2020 [PMID: 33238133] (WB, Mouse)
  
  Details:
  Naïve T cells from the lymph nodes and spleens of C57BL/6 mice, whose Gfm1 gene had been deleted, underwent Western blot analysis.
• GOmez-Lado N, LOpez-Arias E, Iglesias-Rey R et al. [18F]-FMISO PET/MRI Imaging Shows Ischemic Tissue around Hematoma in Intracerebral Hemorrhage Mol Pharm Nov 13 2020 [PMID: 33186043] (Human)
• Yang, X, Zhong, D Et al. Conditional ablation of MAPK7 expression in chondrocytes impairs endochondral bone formation in limbs and adaptation of chondrocytes to hypoxia. Cell Biosci Sep 19 2020 [PMID: 32944217] (IHC, WB, Mouse)
• Wang N, Shi XF, Khan SA et al. Hypoxia inducible factor-1 mediates pancreatic beta-cell dysfunction by intermittent hypoxia Am. J. Physiol., Cell Physiol. Sep 16 2020 [PMID: 32936698] (ICC/IF, Mouse)
• Kim, C S, Ding, X Et al. Glutamine Metabolism Controls Stem Cell Fate Reversibility and Long-Term Maintenance in the Hair Follicle. Cell Metab Oct 6 2020 [PMID: 32905798] (WB, Mouse)
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (WB, ChIP, Mouse)
• Thomas A, Samykutty A, Gomez-Gutierrez JG et al. Actively Targeted Nanodelivery of Echinomycin Induces Autophagy-Mediated Death in Chemoresistant Pancreatic Cancer In Vivo Cancers (Basel) Aug 14 2020 [PMID: 32823919] (WB, Human)
• Babetto E, Wong KM, Beirowski B A glycolytic shift in Schwann cells supports injured axons Nat. Neurosci. Aug 17 2020 [PMID: 32807950] (ICC/IF, Mouse)
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  Details:
  HIF1 alpha antibody used to show the effect on cells of YC-1 exposure in both normoxic and hypoxic by western blot in mouseNIH/3T3 cells
• Sharma B, Ho L, Ford GH et al. Alternative Progenitor Cells Compensate to Rebuild the Coronary Vasculature in Elabela- and Apj-Deficient Hearts Dev. Cell 2017 Sep 25 [PMID: 28890073] (IHC, Mouse)
• Huang R, Hayashi Y, Yan X et al HIF1A is a critical downstream mediator for hemophagocytic lymphohistiocytosis. Haematologica. 2017 Nov [PMID: 28860338] (Mouse)
  
  Details:
  Citation using the DyLight 405 version of this antibody.
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• Licarete E, Sesarman A, Rauca VF et al. HIF-1a acts as a molecular target for simvastatin cytotoxicity in B16.F10 melanoma cells cultured under chemically induced hypoxia. Oncol Lett May 1 2017 [PMID: 28521491] (WB, Mouse)
• Kiriakidis S, Hoer SS, Burrows N et al. Complement C1q is hydroxylated by collagen prolyl 4 hydroxylase and is sensitive to off-target inhibition by prolyl hydroxylase domain inhibitors that stabilize hypoxia-inducible factor. Kidney Int. May 12 2017 [PMID: 28506759] (IB, Mouse)
• Jackson CS, Durandt C, Janse van Rensburg I et al. Targeting the aryl hydrocarbon receptor nuclear translocator complex with DMOG and Stemregenin 1 improves primitive hematopoietic stem cell expansion. Stem Cell Res. Apr 20 2017 [PMID: 28445828]
• Lin Yu-Jung, Shyu Woei-Cherng, Chang Chi-Wei et al. Tumor Hypoxia Regulates Forkhead Box C1 to Promote Lung Cancer Progression. Theranostics 2017 Mar 5 [PMID: 28435457] (ChIP, IHC-Fr, WB, Human)
• Ravesloot-Chavez MM Mechanisms of control in Mycobacterium tuberculosis infection Thesis Jan 1 2022 (WB, Mouse)
• Tokumoto Y, Tamaki S, Kabe Y et al. Quiescence of adult oligodendrocyte precursor cells requires thyroid hormone and hypoxia to activate Runx1. Sci Rep Apr 21 2017 [PMID: 28432293] (ICC/IF, Rat)
• Yamazaki T, Mimura I, Miura R et al. Dznep, a histone modification inhibitor, inhibits HIF1 alpha binding to TIMP2 gene, by reducing open chromatin area Research Square Aug 25 2022 (ChIP)
  
  Details:
  Dilutions used for ChIP 1:50
• Duan C, Perez-Torres CJ, Yuan L et al. Can anti-vascular endothelial growth factor antibody reverse radiation necrosis. A preclinical investigation. J. Neurooncol. Apr 19 2017 [PMID: 28425047]
• Schuoler C, Haider TJ, Leuenberger C et al. Aquaporin 1 controls the functional phenotype of pulmonary smooth muscle cells in hypoxia-induced pulmonary hypertension. Basic Res. Cardiol. May 1 2017 [PMID: 28409279] (WB, Mouse)
• Hum JM, Clinkenbeard EL, Ip C et al. The metabolic bone disease associated with the Hyp mutation is independent of osteoblastic HIF1a expression. Bone Reports. Jan 17 2017 [PMID: 28377980] (WB, Rat)
• Sato Y, Tsuyama T, Sato C et al. Hypoxia reduces HNF4alpha/MODY1 protein expression in pancreatic B-cells by activating AMP-activated protein kinase. J. Biol. Chem. Mar 31 2017 [PMID: 28364040] (WB, Mouse)
• Yao F, Chu M, Xi G et al. Single-Embryo Transcriptomic Atlas of Oxygen Response Reveals the Critical Role of HIF-1 alpha in Prompting Major Zygotic Gene Activation SSRN Electronic Journal Feb 24 2022
• Akhtar R, Tahir H, Stewart E et al. Toll-Like Receptor Signalling Pathways Regulate Hypoxic Stress Induced Fibroblast Growth Factor but Not Vascular Endothelial Growth Factor-A in Human Microvascular Endothelial Cells International Journal of Translational Medicine May 27 2021 (WB, Human)
• Sourabh S, Padwad Y HIF-1 in cancer therapy: two decade long story of a transcription factor. Acta Oncol Jan 4 2017 [PMID: 28358664] (IHC, Human)
• Landfried B, Samardzija M, Barben M et al. Digoxin-induced retinal degeneration depends on rhodopsin. Cell Death Dis. Mar 16 2017 [PMID: 28300845] (WB, Mouse)
• Wang S, Zeng H, Chen ST et al. Ablation of endothelial prolyl hydroxylase domain protein-2 promotes renal vascular remodelling and fibrosis in mice. J. Cell. Mol. Med. Mar 7 2017 [PMID: 28266128] (WB, Mouse)
• Maturu P, Jones D, Ruteshouser EC et al. Role of Cyclooxygenase-2 Pathway in Creating an Immunosuppressive Microenvironment and in Initiation and Progression of Wilms' Tumor. Neoplasia. Mar 1 2017 [PMID: 28254151] (IHC-P, Mouse)
• Asai, Y, Yamada, T Et al. Activation of the Hypoxia Inducible Factor 1 alpha Subunit Pathway in Steatotic Liver Contributes to Formation of Cholesterol Gallstones. Gastroenterology May 1 2017 [PMID: 28088462] (WB, Human)
• Brock M, Schuoler C, Leuenberger C et al. Analysis of hypoxia-induced noncoding RNAs reveals metastasis-associated lung adenocarcinoma transcript 1 as an important regulator of vascular smooth muscle cell proliferation Exp. Biol. Med. (Maywood) Jan 1 2017 [PMID: 28056547] (Mouse)
• Razafinjatovo CF, Stiehl D, Deininger E et al. VHL missense mutations in the p53 binding domain show different effects on p53 signaling and HIFalpha degradation in clear cell renal cell carcinoma. Oncotarget. Feb 7 2017 [PMID: 28052007] (WB, Mouse)
• Negrette-Guzman M, Huerta-Yepez S, Vega MI et al. Sulforaphane induces differential modulation of mitochondrial biogenesis and dynamics in normal cells and tumor cells. Food Chem. Toxicol. Dec 18 2016 [PMID: 27993529] (ICC/IF, Human)
• Myllymaki MN, Maatta J, Dimova EY et al. Notch downregulation and extramedullary erythrocytosis in HIF prolyl 4-hydroxylase-2-deficient mice. Mol. Cell. Biol. Nov 7 2016 [PMID: 27821476]
• Morita M, Sato Y, Iwasaki R et al. Selective Estrogen Receptor Modulators Suppress Hif1alpha Protein Accumulation in Mouse Osteoclasts. PLoS ONE. Nov 1 2016 [PMID: 27802325] (WB, Moues)
• Hoogewijs D, Vogler M, Zwenger E et al. Oxygen-dependent regulation of aquaporin-3 expression. Hypoxia (Auckl). Nov 1 2016 [PMID: 27800511] (Mouse)
• Liu Y, Gu Y, Ng S Et Al. Circulating levels of hydroxylated bradykinin function as an indicator of tissue HIF-1 alpha expression Science Bulletin Jan 1 2020 (WB,EA , Human)
• Guentsch A, Beneke A, Swain L et al. PHD2 Is a Regulator for Glycolytic Reprogramming in Macrophages. Mol. Cell. Biol. Jan 1 2017 [PMID: 27795296]
• Tian X, Nguyen M, Foote HP et al. CRLX101, a nanoparticle-drug conjugate containing camptothecin, improves rectal cancer chemoradiotherapy by inhibiting DNA repair and HIF-1alpha. Cancer Res. Oct 26 2016 [PMID: 27784746]
• Ostergaard L, Rudiger A, Wellmann S et al. Arginine-vasopressin marker copeptin is a sensitive plasma surrogate of hypoxic exposure. Hypoxia (Auckl) 2014 Sep 11 [PMID: 27774473]
• Sousa LM, Silva RD, Fonseca VU et al. Is the canine corpus luteum an insulin-sensitive tissue?. J. Endocrinol. Dec 1 2016 [PMID: 27679426]
• Clever D, Roychoudhuri R, Constantinides MG et al. Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche. Cell. Aug 25 2016 [PMID: 27565342]
• Koivunen P, Fell SM, Lu W et al. The 2-oxoglutarate analog 3-oxoglutarate decreases normoxic hypoxia-inducible factor-1alpha in cancer cells, induces cell death, and reduces tumor xenograft growth. Hypoxia (Auckl) 2016 Mar 04 [PMID: 27525289]
• Pandya PH, Fisher AJ, Mickler EA et al. HIF-1alpha Regulates CD55 in Airway Epithelium. Am J Respir Cell Mol Biol 2016 Aug 5 [PMID: 27494303]
• Guimaraes TA, Farias LC, Santos ES et al. Metformin increases PDH and suppresses HIF-1alpha under hypoxic conditions and induces cell death in oral squamous cell carcinoma. Oncotarget. Jul 26 2016 [PMID: 27474170] (WB, IHC, Human)
• Jung GS, Jeon JH, Choi YK et al. Pyruvate dehydrogenase kinase regulates hepatitis C virus replication. Sci Rep. Jul 29 2016 [PMID: 27471054] (IHC, WB, Human)
• Leinonen H, Rossi M, Salo AM et al. Lack of P4H-TM in mice results in age-related retinal and renal alterations. Hum. Mol. Genet. Jul 27 2016 [PMID: 27466183] (WB, Mouse)
• Luo B, Wang J, Liu Z et al. Phagocyte respiratory burst activates macrophage erythropoietin signalling to promote acute inflammation resolution Nat Commun 2016 Jul 11 [PMID: 27397585] (WB)
• Dai X, Ruan J, Guo Y et al. Enhanced Radiotherapy Efficacy and Induced Anti-tumor Immunity in HCC by Improving Hypoxia Microenvironment using Oxygen Microcapsules Chemical Engineering Journal Apr 1 2021 (ICC/IF, IHC)
• Nunomiya A, Shin J, Kitajima Y et al. Activation of the hypoxia-inducible factor pathway induced by prolyl hydroxylase domain 2 deficiency enhances the effect of running training in mice Acta Physiol (Oxf) 2016 Jul 09 [PMID: 27393382] (WB, Mouse)
• Diaz-Trelles R, Scimia MC, Bushway P et al. Notch-independent RBPJ controls angiogenesis in the adult heart. Nat Commun 2016 Jun 30 [PMID: 27357444] (WB)
• Gamal-Eldeen A, Baghdadi H, Afifi N, et al. Gum arabic-encapsulated gold nanoparticles modulate hypoxamiRs expression in tongue squamous cell carcinoma Molecular & Cellular Toxicology Jan 26 2021
• Xue H, Chen D, Zhong YK et al. Deferoxamine ameliorates hepatosteatosis via several mechanisms in ob/ob mice Ann. N. Y. Acad. Sci. 2016 Jul 01 [PMID: 27447538] (WB, Mouse)
• Aarup A, Pedersen T X et al Hypoxia-Inducible Factor-1 alpha Expression in Macrophages Promotes Development of Atherosclerosis Arterioscler Thromb Vasc Biol Jul 23 2016 [PMID: 27444197] (IHC, Mouse)
  
  Details:
  Citation using the Biotin version of this antibody.
• Braverman J, Sogi KM, Benjamin D et al. HIF-1 alpha Is an Essential Mediator of IFN-gamma-Dependent Immunity to Mycobacterium tuberculosis J. Immunol. 2016 Jul 18 [PMID: 27430718]
• Robl B, Botter SM, Pellegrini G et al. Evaluation of intraarterial and intravenous cisplatin chemotherapy in the treatment of metastatic osteosarcoma using an orthotopic xenograft mouse model J Exp Clin Cancer Res. 2016 Jul 15 [PMID: 27421768] (IHC-P, Mouse)
• Muinos-Lopez E, Ripalda-Cemborain P, Lopez-Martinez T et al. Hypoxia and Reactive Oxygen Species Homeostasis in Mesenchymal Progenitor Cells Define a Molecular Mechanism for Fracture Nonunion. Stem Cells Jun 2 2016 [PMID: 27250101]
• Bullen JW, Tchernyshyov I, Holewinski RJ et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016 May 31 [PMID: 27245613] (WB, Human)
• Halt KJ, Parssinen HE, Junttila SM, Saarela U. CD146+ cells are essential for kidney vasculature development. Kidney International. 2016 Apr 18 [PMID: 27165833] (WB, Mouse)
• Christinat Y, Pawlowski R, Krek W. jSplice: a high-performance method for accurate prediction of alternative splicing events and its application to large-scale renal cancer transcriptome data. Bioinformatics 2016 Jul 15 [PMID: 27153587]
• Ruffenach G, Chabot S, Tanguay VF et al. Role for RUNX2 in Proliferative and Calcified Vascular Lesions in Pulmonary Arterial Hypertension. Am. J. Respir. Crit. Care Med. May 5 2016 [PMID: 27149112] (IHC-P, WB, IP, Rat, Human)
• Takahashi N, Davy PM, Gardner LH et al. Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle. PLoS ONE. May 6 2016 [PMID: 27148974] (IHC-WhMt, ICC/IF, IHC-P, Mouse)
• Msaki A, Pasto A, Curtarello M et al. A hypoxic signature marks tumors formed by disseminated tumor cells in the BALB-neuT mammary cancer model. Oncotarget. May 31 2016 [PMID: 27105499] (WB, Mouse)
• Trinh NT, Yamashita T, Ohneda K et al. Increased Expression of EGR-1 in Diabetic Human Adipose Tissue-Derived Mesenchymal Stem Cells Reduces Their Wound Healing Capacity. Stem Cells Dev 2016 May 15 [PMID: 26988763]
• Shin J, Nunomiya A, Kitajima Y et al. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle 2016 Mar 05 [PMID: 26949511] (WB)
• Ruf M, Moch H, Schraml P. PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma. Int J Cancer 2016 Jul 15 [PMID: 26945902]
• Zhou F, Dai A, Jiang Y et al. SENP1 enhances hypoxiainduced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxiainducible factor1alpha. Mol Med Rep 2016 Apr [PMID: 26935971] (WB)
• Rahtu-Korpela L, Maatta J et al. Hypoxia-Inducible Factor Prolyl 4-Hydroxylase-2 Inhibition Protects Against Development of Atherosclerosis. Arterioscler Thromb Vasc Biol Jan 4 2016 [PMID: 26848160] (WB, Mouse)
• Regan Anderson TM, Ma SH, Raj GV et al. Breast Tumor Kinase (Brk/PTK6) Is Induced by HIF, Glucocorticoid Receptor, and PELP1-Mediated Stress Signaling in Triple-Negative Breast Cancer Cancer Res. Jan 29 2016 [PMID: 26825173] (WB, Human)
• Lee SD, Kim W, Jeong JW et al. AK-1, a SIRT2 inhibitor, destabilizes HIF-1alpha and diminishes its transcriptional activity during hypoxia. Cancer Lett. 2016 Jan 22 [PMID: 26808575] (ICC/IF, Human)
  
  Details:
  HIF1 alpha antibody was used for immunofluorescence/ICC-IF staining of A549 cells which were exposed to hypoxia for six hours in the presence of a SIRT2-specific inhibitor (10uM AK-1 ).
• Qian F, He M, Duan W et al. Cross regulation between hypoxia-inducible transcription factor-1alpha (HIF-1alpha) and transforming growth factor (TGF)-ss1 mediates nickel oxide nanoparticles (NiONPs)-induced pulmonary fibrosis. Am J Transl Res 2015 [PMID: 26807184] (WB)
• Tando T, Sato Y, Miyamoto K et al. Hif1alpha is required for osteoclast activation and bone loss in male osteoporosis. Biochem. Biophys. Res. Commun. 2016 Feb 05 [PMID: 26792721] (WB, IHC-P, Mouse)
  
  Details:
  HIF1 alpha antibody was used for IHC-P analysis of femurs from orchidectomy/ORX or sham-operated Wild type C57BL/6 mice - the bones fixed 10% formalin, decalcified in 8% Na2EDTA, antigen was retrieved for 8 minutes at RT with 0.4 mg/mL Proteinase K and primary detected using Alexa488-conjugated goat anti-rabbit secondary (Fig. 1). The antibody was also used for WB on lysates of Raw264.7 cells cultured with or without testosterone in normoxic or hypoxic conditions.
• Kinoshita T, Fujii H, Hayashi Y et al. Prognostic significance of hypoxic PET using (18)F-FAZA and (62)Cu-ATSM in non-small-cell lung cancer. Lung Cancer 2016 Jan [PMID: 26711935]
• Laukka T, Mariani CJ, Ihantola T et al. Fumarate and Succinate Regulate Expression of Hypoxia-inducible Genes via TET Enzymes. J Biol Chem 2016 Feb 19 [PMID: 26703470]
• Aquino-Galvez A, Gonzalez-avila G. Effects of 2-methoxyestradiol on apoptosis and HIF-1a and HIF-2a expression in lung cancer cells under normoxia and hypoxia. ONCOLOGY REPORTS. 2015 Aug 20 [PMID: 26548300] (WB, ICC/IF, Human)
• Zhang J, Wang C, Chen X et al. EglN2 associates with the NRF1-PGC1alpha complex and controls mitochondrial function in breast cancer. EMBO J 2015 Dec 02 [PMID: 26492917] (WB, Human)
• Karsikas S, Myllymaki M, Heikkila M et al. HIF-P4H-2 deficiency protects against skeletal muscle ischemia-reperfusion injury. J Mol Med (Berl) 2016 Mar [PMID: 26452676]
• Saini Y, Proper SP, Dornbos P et al. Loss of Hif-2alpha Rescues the Hif-1alpha Deletion Phenotype of Neonatal Respiratory Distress In Mice. PLoS One 2015 [PMID: 26422241] (IHC, Mouse)
• Bustamante-Marin XM, Cook MS, Gooding J et al. Left-Biased Spermatogenic Failure in 129/SvJDnd1Ter/+ Mice Correlates with Differences in Vascular Architecture, Oxygen Availability, and Metabolites 1 Biol Reprod. 2015 Feb 06 [PMID: 26224005] (WB, Mouse)
• Zeng Z, Huang WD, Gao Q et al. Arnebin-1 promotes angiogenesis by inducing eNOS, VEGF and HIF-1a expression through the PI3K-dependent pathway Int. J. Mol. Med. 2015 Jul 22 [PMID: 26202335] (WB, Human)
• Chen X, Liu J, He B et al. Vascular endothelial growth factor (VEGF) regulation by hypoxia inducible factor-1 alpha (HIF1A) starts and peaks during endometrial breakdown, not repair, in a mouse menstrual-like model Hum. Reprod. 2015 Jun 25 [PMID: 26113661] (IHC-P, Mouse)
  
  Details:
  HIF1 alpha antibody was used at 1:100 dilution for IHC-P analysis of 5um paraffin sections of uteri tissues from mouse menstrual-like model (details in Fan et al Faseb J 2008 22 3571- 3580).
• Liu J, You P, Chen G et al. Hyperactivated FRS2a-mediated signaling in prostate cancer cells promotes tumor angiogenesis and predicts poor clinical outcome of patients Oncogene. 2015 Jun 22 [PMID: 26096936] (WB, Human)
• Mirtschink P, Krishnan J, Grimm F et al. HIF-driven SF3B1 induces KHK-C to enforce fructolysis and heart disease Nature. 2015 Jun 25 [PMID: 26083752] (WB, Mouse)
• Guo C, Zhang Y, Wang T, Zhong M. Intranasal deferoxamine attenuates synapse loss via up?regulating the P38/HIF?1alpha pathway on the brain of APP/PS1 transgenic mice. Frontiers in Aging Neuroscience. 2015 Jun 02 [PMID: 26082716] (IHC-P, WB, Mouse)
• Zhao H, Wu Y, Chen Y, Liu H. Clinical significance of hypoxia-inducible factor 1 and VEGF-A in osteosarcoma Int. J. Clin. Oncol. 2015 Jun 16 [PMID: 26077139] (IHC-P, WB, Human)
• Xie L, Pi X, Townley-Tilson WH et al. PHD2/3-dependent hydroxylation tunes cardiac response to beta-adrenergic stress via phospholamban. J Clin Invest 2015 Jul 1 [PMID: 26075818] (WB)
• Yorulmaz H, Ozkok E, Erguven M et al. Effect of simvastatin on mitochondrial enzyme activities, ghrelin, hypoxia-inducible factor 1alpha in hepatic tissue during early phase of sepsis. Int J Clin Exp Med 2015 [PMID: 26064259] (IHC, Rat)
• Zieseniss A, Hesse AR, Jatho A et al. Cardiomyocyte-Specific Transgenic Expression of Prolyl-4-Hydroxylase Domain 3 Impairs the Myocardial Response to Ischemia. Cell Physiol Biochem 2015 May 27 [PMID: 26044310]
• Hwang HJ, Lynn SG, Vengellur A et al. Hypoxia Inducible Factors Modulate Mitochondrial Oxygen Consumption and Transcriptional Regulation of Nuclear-Encoded Electron Transport Chain Genes. Biochemistry 2015 Jun 23 [PMID: 26030260]
• Valbuena GN, Rizzardini M, Cimini S et al. Metabolomic Analysis Reveals Increased Aerobic Glycolysis and Amino Acid Deficit in a Cellular Model of Amyotrophic Lateral Sclerosis. Mol. Neurobiol. 2015 May 12 [PMID: 25963727] (WB, Human, Mouse)
• Conley SJ, Baker TL, Burnett JP et al. CRLX101, an investigational camptothecin-containing nanoparticle-drug conjugate, targets cancer stem cells and impedes resistance to antiangiogenic therapy in mouse models of breast cancer Breast Cancer Res. Treat. 2015 Apr 01 [PMID: 25833208] (WB, IHC, Human)
• Tan SC, Gomes RS, Yeoh KK et al. Preconditioning of cardiosphere-derived cells with hypoxia or prolyl-4-hydroxylase inhibitors increases stemness and decreases reliance on oxidative metabolism. Cell Transplant. 2015 Mar 06 [PMID: 25751158] (WB, Rat)
• Albers J, Danzer C, Rechsteiner M et al. A versatile modular vector system for rapid combinatorial mammalian genetics. J Clin Invest 2015 Apr [PMID: 25751063] (WB)
• Lee M, Choi SY, Kang BH et al. Differential microscopic finding and glucose transporter 3 expression in terminal chorionic villi among birth weight-discordant twin placentas Histol. Histopathol. 2015 Mar 02 [PMID: 25728965] (IHC-P)
• Kato H, Izumi K, Uenoyama A et al. Hypoxia Induces an Undifferentiated Phenotype of Oral Keratinocytes in vitro Cells Tissues Organs (Print). 2015 Feb 18 [PMID: 25720390]
• Qiao N, Xu C, Zhu YX et al. Ets-1 as an early response gene against hypoxia-induced apoptosis in pancreatic beta-cells Cell Death Dis. 2015 Feb 19 [PMID: 25695603]
• Stantic M, Sakil HA, Zirath H et al. TAp73 suppresses tumor angiogenesis through repression of proangiogenic cytokines and HIF-1alpha activity.. Proc Natl Acad Sci U S A. 2015 Jan 6 [PMID: 25535357] (WB,ChIP)
• Sato Yoshifumi, Inoue Masahiro, Yoshizawa Tatsuya, Yamagata Kazuya. Moderate hypoxia induces beta-cell dysfunction with HIF-1-independent gene expression changes. PLoS One. 2014 Dec 12 [PMID: 25503986] (WB)
• Takamiya R, Takahashi M, Uehara Y, Ariki S. The single N-glycan deletion mutant of soluble ErbB3 protein attenuates heregulinb1-induced tumor progression by blocking of the HIF-1 and Nrf2 pathway. Biochem Biophys Res Commun. 2014 Oct 24 [PMID: 25451255] (WB, Human)
  
  Details:
  HIF1 alpha antibody used for WB on experiments involving MCF7, T47D and BT474 cells
• Fine Jm, Renner Db, Forsberg Ac et al. Intranasal deferoxamine engages multiple pathways to decrease memory loss in the APP/PS1 model of amyloid accumulation. Neurosci. Lett. 2014 Nov 13 [PMID: 25445365] (WB, Mouse)
• Xie Yuan, Zhang Jin, Lin Ying et al. Defining the role of oxygen tension in human neural progenitor fate. Stem Cell Reports. 2014 Nov 11 [PMID: 25418722] (ICC/IF, Human)
• Lam Gc, Sefton Mv. Tuning Graft- and Host-Derived Vascularization in Modular Tissue Constructs: A Potential Role of HIF1 Activation Tissue Eng Part A et al. 2014 Nov 07 [PMID: 25379774] (IHC-P, Human)
  
  Details:
  HIF1 alpha antibody used for IHC-P on human modular tissues /sub-millimeter collagen modules containing adipose-derived mesenchymal stromal cells (adMSC) and enveloped by human umbilical vein endothelial cells (HUVEC) subcutaneously implanted in severe-combined immunodeficient mice with a beige-mutation (SCID-bg) mice - 24 hour fixation in 10% neutral buffered formalin, 4um paraffin sections, 1:2000 primary dilution,
• Clarke Rh, Moosa S, Anzivino M et al. Sustained radiosensitization of hypoxic glioma cells after oxygen pretreatment in an animal model of glioblastoma and in vitro models of tumor hypoxia. PLoS OnE. 2014 Oct 29 [PMID: 25350400] (WB, Human)
• Takahashi H, Inoue A, Kawabe Y et al. Oct-3/4 promotes tumor angiogenesis through VEGF production in glioblastoma Brain Tumor Pathol et al. 2014 Oct 28 [PMID: 25348671] (WB, ICC/IF, Human)
  
  Details:
  HIF1 alpha antibody used for WB and ICC-IF on U251/EGFP or U251/EGFP-Oct-3/4 cells (Fig 5). WB also performed on cells subjected to Oct-3/4 knockdown (Fig 6b) and U251/EGFP-Oct-3/4 cells were treated with AKT inhibitor (Fig 6c).
• Burgi S, Seuwen A, Keist R et al. In Vivo Imaging of Hypoxia-Inducible Factor Regulation in a Subcutaneous and Orthotopic GL261 Glioma Tumor Model Using a Reporter Gene Assay. Mol Imaging. 2014 Sep 01 [PMID: 25248521]
• Okon IS, Coughlan KA, Zhang C et al. Protein kinase LKB1 promotes RAB7-mediated neuropilin-1 degradation to inhibit angiogenesis. J Clin. Invest. 2014 Sep 02 [PMID: 25180605] (WB, IP, Human)
• Huang BW, Miyazawa M, Tsuji Y et al. Distinct Regulatory Mechanisms of the Human Ferritin Gene by Hypoxia and Hypoxia Mimetic Cobalt Chloride at the Transcriptional and Post-transcriptional Levels. Cell. Signal. 2014 Aug 26 [PMID: 25172425] (WB, Human)
• Hesse AR, Levent E, Zieseniss A et al. Lights on for HIF-1a: Genetically Enhanced Mouse Cardiomyocytes for Heart Tissue Imaging. Cell. Physiol. Biochem. 2014 Jul 30 [PMID: 25095893] (WB, Mouse)
  
  Details:
  HIF-1 alpha antibody used for WB on protein extracts of hypoxia exposed ODD-Luc-CMs/Cardiomyocytes derived from an embryonic stem cell (ESC) lines (ODD-Luc ESCs) that was generated from a Tg ROSA26 ODD-Luc/+ mouse (Figure 2D).
• Kaasinen E, Rahikkala E, Koivunen P et al. Clinical characterization, genetic mapping and whole-genome sequence analysis of a novel autosomal recessive intellectual disability syndrome. Eur J Med Genet 2014 Jul 28 [PMID: 25078763] (WB, Human)
  
  Details:
  HIF1a/HIF-1 alpha antibody used for WB on proteins extracts from human lymphoblasts cultured under normoxic and hypoxic conditions. Lymphoblasts were from six patients who developed an unexplained syndromic form of profound intellectual disability (Supplementary Fig. 2b and c).
• Ueda J, Ho JC, Lee KL et al. The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. Mol. Cell. Biol. 2014 Jul 28 [PMID: 25071150] (WB, Mouse)
  
  Details:
  HIF1 alpha antibody used in WB on 1% hypoxia exposed wild-type murine embryonic stem cells (Fig 4A - HIF1a used as positive control for hypoxic induction).
• Abu-Remaileh M, Aqeilan RI. Tumor suppressor WWOX regulates glucose metabolism via HIF1alpha modulation. Cell Death Differ 2014 Nov [PMID: 25012504] (WB)
• Slominski A, Kim TK, Brozyna AA et al. The role of melanogenesis in regulation of melanoma behavior: Melanogenesis leads to stimulation of HIF-1a expression and HIF-dependent attendant pathways. Arch. Biochem. Biophys. 2014 Jul 02 [PMID: 24997364] (WB, Human, Hamster)
  
  Details:
  HIF-1 alpha antibody used for WB on whole cell lysates of Bomirski hamster AbC1 and human SKMEL-188 stable lined (amelanotic /non-pigmented and melanotic /pigmented lines) with Hif1a wild type/WT and knockout /KO mammary tumor epithelial cells (MTECs) derived from the polyoma virus middle T transgenic mouse (MMTV-PyMT) as positive control for HIF-1 alpha. Antibody used at 1:5000 dilution and the data is shown in Fig 1.
• Kono K, Tamashiro DA, Alarcon VB. Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of hippo signaling in the mouse blastocyst. Dev. Biol. 2014 Jul 02 [PMID: 24997360] (WB, Human, Hamster)
  
  Details:
  HIF-2 alpha antibody used for WB on lysates of Bomirski hamster AbC1 and human SKMEL-188 cell lines (Fig. 1).
• Zheng Xingnan, Zhai Bo, Koivunen Peppi et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014 Jul 01 [PMID: 24990963] (WB, Mouse)
• Chen RL, Ogunshola OO, Yeoh KK et al. HIF prolyl hydroxylase inhibition prior to transient focal cerebral ischaemia is neuroprotective in mice J. Neurochem. 2014 Jun 26 [PMID: 24974727] (IHC-P, WB, Mouse, Rat)
  
  Details:
  HIF-1 alpha antibody used for IHC-P on FFPE tissue sections of C57/B6 mouse brains (in vivo transient focal cerebral ischaemia model) treated or not with 2-(1-chloro-4-hydroxyisoquinoline-3-carboxamido) acetic acid (IOX3), a selective small molecule inhibitor of hypoxia-inducible factor (HIF) prolyl hydroxylases (staining images - Figure 4). Antibody also used for WB on rat brain endothelial cell line RBE4 exposed to IOX3 and hypoxia (Figure 6)
• Peng YJ, Yuan G, Khan S et al. Regulation of Hypoxia Inducible Factor-alpha Isoforms and Redox State By Carotid Body Neural Activity in Rats. J. Physiol. (Lond.). 2014 Jun 27 [PMID: 24973414] (IHC-Fr, WB, Rat)
• Mangiavini L, Merceron C, Araldi E et al. Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development Dev. Biol. 2014 Jun 24 [PMID: 24972088] (WB, Mouse)
  
  Details:
  HIF-1 alpha antibody used for Western blot at 1:1000 dilution in protein lysate from chondrocytes of CNTRL and VHL mice ( protocol details provided in this article, WB image - Supplemental Fig. 1, Panel B)
• Wojtal K A, Cee A et al. Downregulation of duodenal SLC transporters and activation of proinflammatory signaling constitute the early response to high altitude in humans. Am J Physiol Gastrointest Liver Physiol Jan 10 2014 [PMID: 24970780] (WB, Human)
• Ghim J, Moon J S et al. Endothelial deletion of phospholipase D2 reduces hypoxic response and pathological angiogenesis. Arterioscler Thromb Vasc Biol Jan 8 2014 [PMID: 24947526] (WB, Mouse)
• Gupta PK, DiPette DJ, Supowit SC Protective effect of resveratrol against pressure overload?induced heart failure. Food Sci Nutr 2014 Mar 5 [PMID: 24936291] (WB, Mouse)
• Rahtu-Korpela Lea, Karsikas Sara, Horkko Sohvi et al. HIF prolyl 4-hydroxylase-2 inhibition improves glucose and lipid metabolism and protects against obesity and metabolic dysfunction. Diabetes. 2014 May 01 [PMID: 24789921] (Mouse)
• Yu Z, Ge Y, Xie L et al. Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1alpha in HepG2 cells. Cell. Signal. 2014 Mar 29 [PMID: 24686083] (WB, IP, Human)
• Chen X, Iliopoulos D, Zhang Q et al. XBP1 promotes triple-negative breast cancer by controlling the HIF1a pathway. Nature 4/3/2014 [PMID: 24670641] (IP, Human)
• Mochizuki A, Pace A, Rockwell CE et al. Hepatic Stellate Cells Orchestrate Clearance of Necrotic Cells in a Hypoxia-Inducible Factor-1a-Dependent Manner by Modulating Macrophage Phenotype in Mice. J. Immunol. 3/17/2014 [PMID: 24639359] (IHC-Fr, Mouse)
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  Details:
  Using the HRP conjugated version of NB100-479, catalog number NB100-479H.
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• Thiersch M, Lange C, Joly S et al. Retinal neuroprotection by hypoxic preconditioning is independent of hypoxia-inducible factor-1 alpha expression in photoreceptors. Eur J Neurosci 2009 Jun [PMID: 19508692] (WB, Mouse)
• Seavey MM, Maciag PC, Al-Rawi N et al. An anti-vascular endothelial growth factor receptor 2/fetal liver kinase-1 Listeria monocytogenes anti-angiogenesis cancer vaccine for the treatment of primary and metastatic Her-2/neu+ breast tumors in a mouse model. J Immunol 182(9):5537-46. 2009 May 1. [PMID: 19380802] (ICC/IF, IHC, Mouse)
• Zhang J, Liu A, Hou R et al. Salidroside protects cardiomyocyte against hypoxia-induced death: a HIF-1alpha-activated and VEGF-mediated pathway. Eur J Pharmacol 2009 Apr 1 [PMID: 19326475] (Rat)
• Ishikawa T, Nakashiro K, Klosek SK et al. Hypoxia enhances CXCR4 expression by activating HIF-1 in oral squamous cell carcinoma. Oncol Rep 2009 Mar [PMID: 19212630] (WB, Human)
• Wirthner R, Wrann S, Balamurugan K et al. Impaired DNA double-strand break repair contributes to chemoresistance in HIF-1 alpha-deficient mouse embryonic fibroblasts. Carcinogenesis 29(12):2306-16. 2008 Dec. [PMID: 18842680] (WB, Mouse)
• Deli L, Wittmann G, Kallo I et al. Type 1 cannabinoid receptor-containing axons innervate hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons. Endocrinology 2009 Jan [PMID: 18818298] (IHC, Mouse)
• Saini Y, Harkema JR, LaPres JJ. HIF1alpha is essential for normal intrauterine differentiation of alveolar epithelium and surfactant production in the newborn lung of mice. J Biol Chem 283(48):33650-7. 2008 Nov 28. [PMID: 18801745] (WB, Mouse)
• Ream MA, Chandra R, Peavey M et al. High oxygen prevents fetal lethality due to lack of catecholamines. Am J Physiol Regul Integr Comp Physiol 295(3):R942-53. 2008 Sep. [PMID: 18635452] (WB, Mouse)
• Thiersch M, Raffelsberger W, Frigg R et al. Analysis of the retinal gene expression profile after hypoxic preconditioning identifies candidate genes for neuroprotection. BMC Genomics 2008 Feb 8 [PMID: 18261226] (WB, Mouse)
• Minamishima YA, Moslehi J, Bardeesy N et al. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood 111(6):3236-44. 2008 Mar 15. [PMID: 18096761] (WB, Mouse)
• Liao D, Corle C, Seagroves TN et al. Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression. Cancer Res 67(2):563-72. 2007 Jan 15. [PMID: 17234764] (WB, Mouse)
• Ramanathan M, Pinhal-Enfield G, Hao I et al. Synergistic up-regulation of vascular endothelial growth factor (VEGF) expression in macrophages by adenosine A2A receptor agonists and endotoxin involves transcriptional regulation via the hypoxia response element in the VEGF promoter. Mol Biol Cell 18(1):14-23. 2007 Jan. [PMID: 17065555] (WB, Mouse)
• McLaren AT, Marsden PA, Mazer CD et al. Increased expression of HIF-1alpha, nNOS, and VEGF in the cerebral cortex of anemic rats. Am J Physiol Regul Integr Comp Physiol 292(1):R403-14. 2007 Jan. [PMID: 16973934] (WB, IHC, ICC/IF, Rat)
• Kariuki TM, Van Dam GJ, Deelder AM et al. Previous or ongoing schistosome infections do not compromise the efficacy of the attenuated cercaria vaccine. Infect Immun 2006 Jul [PMID: 16790771]
• Bayele HK, Peyssonnaux C, Giatromanolaki A et al. HIF-1 regulates heritable variation and allele expression phenotypes of the macrophage immune response gene SLC11A1 from a Z-DNA forming microsatellite. Blood 110(8):3039-48. 2007 Oct 15. [PMID: 1760676] (WB, Mouse)
• Kundu CN, Balusu R, Jaiswal AS et al. Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis 28(10):2089-95. 2007 Oct. [PMID: 1752206] (WB, Mouse)

Reviews for HIF-1 alpha Antibody (NB100-479) (28)

Average Rating: 4.4

(Based on 28 reviews)

We have 28 reviews tested in 6 species: Human, Mouse, Rat, Human and Human Breast Cancer Cell Lines, Human and Mouse, Other.

reviewed by: PRAVEEN Dubey WB Human 05/28/2021

Summary

• Application: Western Blot
• Sample Tested: A549 cells
• Species: Human
• Lot: AQ-2

reviewed by: Shaima Salman WB Human 03/15/2021

Summary

• Application: Western Blot
• Sample Tested: Hep3B human hepatocellular carcinoma cell line
• Species: Human
• Lot: AN-1

reviewed by: Verified Customer WB Human and Mouse 10/10/2020

Summary

• Application: Western Blot
• Sample Tested: cell line, whole cell lysate
• Species: Human and Mouse
• Lot: AP-2

reviewed by: Jie Lan ChIP Human and Human Breast Cancer Cell Lines 05/17/2020

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: Breast cancer cells
• Species: Human and Human Breast Cancer Cell Lines
• Lot: AN-1

reviewed by: Shubham Dubey WB Human 04/16/2019

Summary

• Application: Western Blot
• Sample Tested: 16-HBE
• Species: Human
• Lot: AL-6

reviewed by: L P WB Human 09/18/2018

Summary

• Application: Western Blot
• Sample Tested: Human Cancer Cells
• Species: Human
• Lot: AN-1

reviewed by: Serina Ng IHC-P Mouse 10/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Lot: AK-4

reviewed by: Verified Customer WB Human 02/23/2017

Summary

• Application: Western Blot
• Sample Tested: U251 glioma cell line; whole cell lysate
• Species: Human

Comments

• Comments: 4 hour hypoxia (0.5% oxygen) incubation

reviewed by: Kim Lab WB Mouse 09/21/2016

Summary

• Application: Western Blot
• Sample Tested: Mouse kidney
• Species: Mouse

reviewed by: Liyong Zhang WB Mouse 08/06/2016

Summary

• Application: Western Blot
• Species: Mouse

reviewed by: Verified Customer WB Mouse 10/05/2015

Summary

• Application: Western Blot
• Sample Tested: mouse retina
• Species: Mouse
• Lot: NB100-479

reviewed by: Haiquan Lu WB Human 08/23/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

reviewed by: Qing Zhang WB Human 03/27/2015

Summary

• Application: Western Blot
• Sample Tested: Hela lysates treated with MG132
• Species: Human

reviewed by: Pierre Thiebaut WB Mouse 02/18/2015

Summary

• Application: Western Blot
• Sample Tested: Mouse heart
• Species: Mouse
• Lot: J1

reviewed by: Ning Wang ChIP Human 12/15/2014

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: HEK293
• Species: Human
• Lot: AA-1

reviewed by: cynthia morimoto WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23232625
• Species: Mouse

reviewed by: Ning Wang WB Other 09/15/2014

Summary

• Application: Western Blot
• Sample Tested: SK-N-BE(2) whole cell lysate
• Species: Other
• Lot: AA-1

reviewed by: Verified Customer WB Human 09/12/2014

Summary

• Application: Western Blot
• Sample Tested: HEK293 cell lysate
• Species: Human

reviewed by: Ning Wang WB Other 08/18/2014

Summary

• Application: Western Blot
• Sample Tested: PC12 whole cell lysate
• Species: Other
• Lot: AA-1

reviewed by: Verified Customer WB Human 08/15/2014

Summary

• Application: Western Blot
• Sample Tested: Human Pancreatic Cancer Cell line
• Species: Human
• Lot: AA

reviewed by: Verified Customer WB Human 07/18/2014

Summary

• Application: Western Blot
• Sample Tested: Nuclear fraction, Human cell line
• Species: Human

reviewed by: Verified Customer WB Human 10/29/2013

Summary

• Application: Western Blot
• Sample Tested: Human MCF7 breast epithelial cells
• Species: Human
• Lot: Lot X-1

reviewed by: Ian Darby IHC-P Mouse 06/27/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: paraffin section of skin wound
• Species: Mouse

reviewed by: Verified Customer WB Rat 02/05/2012

Summary

• Application: Western Blot
• Sample Tested: PC12 nuclear extract, Sample Amount: 50ug
• Species: Rat

reviewed by: Naoharu Takano WB Rat 01/03/2012

Summary

• Application: Western Blot
• Sample Tested: PC12 cell whole lysate, Sample Amount: 25ug
• Species: Rat
• Lot: P1

reviewed by: Yves Heremans IHC-P Mouse 07/30/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: normal mouse kidney
• Species: Mouse

reviewed by: Verified Customer IHC-P Human 03/01/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Kidney tumor
• Species: Human
• Lot: I

reviewed by: Verified Customer WB Mouse 02/01/2011

Summary

• Application: Western Blot
• Sample Tested: 4T1 cells whole cell lysate
• Species: Mouse

FAQs for HIF-1 alpha Antibody (NB100-479). (Showing 1 - 10 of 17 FAQs).

1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
   • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
   • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
3. I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg? 
   • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
   • The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.
5. We got the Hif1a (https://www.novusbio.com/products/hif-1-alpha-antibody-h1alpha67_nb100-105 ) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.
   • HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative:https://www.novusbio.com/support/hypoxia-and-hif-faqsAlso, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)
6. I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?
   • A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs: https://www.novusbio.com/support/hypoxia-and-hif-faqs
7. I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.
   • (1)    HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2)    The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3)    Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4)    For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs https://www.novusbio.com/support/hypoxia-and-hif-faqs(5)    Last but not the least, Novus technical support team may be contacted at: technical@novusbio.com 
8. I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!
   • You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.
9. I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
   • CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs: http://www.novusbio.com/support/hypoxia-and-hif-faqs
10. I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
    • CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs: https://www.novusbio.com/support/hypoxia-and-hif-faqs
11. Is cross-reactivity with HIF-2 alpha tested/predicted?
    • Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.
12. Do you sell a positive and negative control for the HIF-1 alpha antibody (NB100-479)?
    • For Western blot, the best positive control that we have is a CoCl2-treated Cos-7 cell lysate, catalog # NB800-PC26. The kit comes with a negative control as well. If you are doing ICC, then you could treat your cells with CoCl2 or expose them to hypoxic conditions.
13. What do think the blocking buffer should be?
    • We recommend 5% non-fat dry milk in TBS.
14. We need to examine tissue-specific knockout of HIF-1a in mouse lungs. We have generated smooth muscle specific knockout of HIF-1a, and would like to demonstrate the knockdown in lung tissue sections by IHC or immunofluorescence. The problem is that all the antibodies for the mouse seem to have too much non-specific staining. Can you recommend an antibody that would be good for mouse lung tissue IHC or immunofluorescence?
    • The antibody with Catalog # NB100-479 is one of our best sellers and has 5 star rating in customer reviews. Please view the product datasheet to see the publications in which researchers have cited this product, over 10 of which are involved in mouse models.
15. Is there a WB image for this antibody?
    • We do have a WB image for the antibody base product. It looks like they ordered an HRP conjugated format, and that specific format page does not have the image. We do all of our testing on the base product.
16. Will this antibody detect HIF-1 alpha protein by WB on a breast cancer cell line in normal condition (without hypoxia)?
    • If the breast cancer gene turned on the HIF1 alpha gene, there should be some expression of the protein, even without hypoxic induction. However, this expression may be very low, and depending on the length of time it took to lyse and/or fix the cells, may have degraded in the presence of oxygen. This may also be affected by the presence or lack of any HIF stabilizers in the lysate buffer. We talk about these stabilizers and other testing protocols in our white paper here: http://www.novusbio.com/whitepapers/cellular-response-hypoxia.html. I would recommend trying to detect HIF expression in cancer cell lines that you run both positive and negative controls along side the cell lines to ensure that any bands detected are in fact HIF1 alpha.
17. Does HIF-1 alpha antibody NB100-479 cross react with HIF-2?
    • None of the QC experiments conducted by the lab showed substantial background/band similar to HIF-2a. Additionally, a BLAST search comparing the immunogen of NB100-479 and the amino acid sequence of human HIF-1 alpha shows the homology between the two is low.
Show All 17 FAQs.

Bioinformatics

• Gene Symbol: HIF1A
• Entrez:
  • 3091(Human)
  • 15251(Mouse)
  • 29560(Rat)
• Uniprot:
  • Q61221(Mouse)