HIF-1 alpha Antibody

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis of HIF-1 alpha in mouse hepatocytes (left lane: normoxic and right lane: hypoxic) using HIF-1 alpha antibody. Image from verified customer review.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.

Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Staining of human placenta, villi.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-479] - Detection of HIF-1 Alpha (green) in RCC4 cells using NB100-479. Nuclei (Blue) were counterstained using Hoechst 33258.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in paraffin section of mouse skin wound using NB100-479 at 1:6,000. Image courtesy of product review by Ian Darby of RMIT University.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Comparison of HIF-1 alpha levels in HMC-1.2 cells and CBMC cells under normoxic and hopoxic conditions. DFX was used as a positive control. [PMID: 20808808] Image using the HRP form of this antibody.

Western Blot: HIF-1 alpha Antibody [NB100-479] - U251 cells exposed to 4 hour hypoxia (0.5% oxygen) incubation. This image was submitted via customer Review.

Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Analysis in normal mouse kidney. Image courtesy of product review by Yves Heremans.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in human kidney tumor.

• Reactivity: Hu, Mu, Rt, Fi, Ha, Pm, Rb
• Applications: WB, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HIF-1 alpha Antibody Summary

• Immunogen: A fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [UniProt# Q61221].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Frozen 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: WB recognizes a band in hypoxic samples at ~115 kDa. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples. HIF-1 alpha protein NBC1-18422 has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479 (refer to product review by Yves Heremans on the datasheet of NBC1-18422). Flow Cytometry usage was reported in multiple scientific literature. Use in Immunoblotting reported in scientific literature (PMID 28506759).
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  Pancreas Lysate (NB820-59244)
  COS-7 Lysate (NB800-PC26)
  Bone Marrow Lysate (NB820-59283)
  HeLa Lysate (NBP2-36450)
  HepG2 Lysate (NBP2-36451)
  HeLa Lysate (NBP2-36452)
  HepG2 Lysate (NBP2-36453)
  HIF-1 alpha Lysate (NBP2-64982)

• Control Peptide:

  HIF-1 alpha Recombinant Protein (NBC1-18422)

Reactivity Notes

Hamster reactivity was reported in scientific literature (PMID: 24997364 and 24997360). Fish reactivity reported in scientific literature (PMID: 29203887).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HIF-1 alpha Antibody

• ARNT-interacting protein
• Basic-helix-loop-helix-PAS protein MOP1
• BHLHE78
• Class E basic helix-loop-helix protein 78
• HIF 1A
• HIF1 alpha
• HIF-1 alpha
• HIF1A
• HIF-1a
• HIF-1-alpha
• HIF1-alpha
• hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor)
• hypoxia-inducible factor 1-alpha
• Member of PAS protein 1
• member of PAS superfamily 1
• MOP1HIF1-ALPHA
• PAS domain-containing protein 8
• PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)

Background

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VHL (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIF-1 alpha Antibody (NB100-479)(266)

Publications using NB100-479

• Eskla KL, Porosk R, Reimets R et al. Hypothermia augments stress response in mammalian cells. Free Radic. Biol. Med. Apr 25 2018 [PMID: 29704622] (WB)
• Raykhel I, Moafi F, Myllymaki SM et al. BAMBI is a novel HIF1-dependent modulator of TGFb-mediated disruption of cell polarity in hypoxia. J. Cell. Sci. Apr 23 2018 [PMID: 29685894] (Canine)
• Barben M, Ail D, Storti F et al. Hif1a inactivation rescues photoreceptor degeneration induced by a chronic hypoxia-like stress. Cell Death Differ. Apr 17 2018 [PMID: 29666476] (WB, Human)
• Williams AL, Khadka V, Tang M et al. HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction. Physiol. Genomics Apr 13 2018 [PMID: 29652636] (Mouse)
• Wang T, Wei W, Mansai HAA et al. Overexpression of appoptosin promotes mitochondrial damage in MIN6 cells. Mol Med Rep. Mar 16 2018 [PMID: 29568862] (WB, Mouse)
• Cui H, Banerjee S, Guo S et al. IFN Regulatory Factor 2 Inhibits Expression of Glycolytic Genes and Lipopolysaccharide-Induced Proinflammatory Responses in Macrophages. J. Immunol. Mar 21 2018 [PMID: 29563175] (WB)
• Zhu N, Yi B, Guo Z et al. Pim-1 Kinase Phosphorylates Cardiac Troponin I and Regulates Cardiac Myofilament Function Cell Physiol Biochem 2018 Mar 10 [PMID: 29544221] (WB, Mouse)
• Conotte S, Tassin A, Conotte R et al. Metabonomic profiling of chronic intermittent hypoxia in a mouse model. Respir Physiol Neurobiol. Mar 6 2018 [PMID: 29522877] (WB, Mouse)
• Barben M, Schori C, Samardzija M, Grimm C. Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia. Mol Neurodegener. Mar 7 2018 [PMID: 29514656] (WB, Mouse)
• Wong K, Beirowski B. Multiple lines of inhibitory feedback on AKT kinase in Schwann cells lacking TSC1/2 hint at distinct functions of mTORC1 and AKT in nerve development. Communicative & Integrative Biology. 2018 Jan 02 [PMID: 29497474] (WB, Mouse)
• Takai K, Drain AP, Lawson DA et al. Discoidin domain receptor 1 (DDR1) ablation promotes tissue fibrosis and hypoxia to induce aggressive basal-like breast cancers. Genes Dev. Feb 1 2018 [PMID: 29483153] (ICC/IF, Mouse)
• Lin CC, Kurashige M, Liu Y, Terabayashi T. A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed. Sci Rep. 2018 Feb 09 [PMID: 29426897] (WB, Human)
• Lennikov A, Mirabelli P, Mukwaya A et al. Selective IKK2 inhibitor IMD0354 disrupts NF-kB signaling to suppress corneal inflammation and angiogenesis. Angiogenesis May 1 2018 [PMID: 29332242] (IHC, Human)
• Buart S, Terry S, Noman MZ et al. Transcriptional response to hypoxic stress in melanoma and prognostic potential of GBE1 and BNIP3. Oncotarget 2017 Oct 30 [PMID: 29312568] (IHC-P, Human)
• Liu L, Liu W, Wang L et al. Hypoxia-inducible factor 1 mediates intermittent hypoxia-induced migration of human breast cancer MDA-MB-231 cells. Oncol Lett 2017 Dec 01 [PMID: 29250173] (WB, Human)
• Tojo K, Tamada N, Nagamine Y et al. Enhancement of glycolysis by inhibition of oxygen-sensing prolyl hydroxylases protects alveolar epithelial cells from acute lung injury. FASEB J. 2017 Dec 12 [PMID: 29233858] (IHC-P, Mouse)
• Heid J, Cencioni C, Ripa R et al. Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health. Sci Rep 2017 Dec 04 [PMID: 29203887] (WB, Fish)
• Carpinteri S, Sampurno S, Malaterre J et al. Experimental study of delivery of humidified-warm carbon dioxide during open abdominal surgery Br J Surg. 2017 Nov 28 [PMID: 29193022] (Mouse)
• Bernard O, Jeny F, Uzunhan Y et al. Mesenchymal stem cells reduce hypoxia-induced apoptosis in alveolar epithelial cells by modulating HIF and ROS hypoxic signalings Am. J. Physiol. Lung Cell Mol. Physiol. 2017 Nov 22 [PMID: 29167125] (WB)
• Sethumadhavan S, Silva M, Philbrook P et al. Hypoxia and hypoxia-inducible factor (HIF) downregulate antigen-presenting MHC class I molecules limiting tumor cell recognition by T cells PLoS ONE. 2017 Nov 20 [PMID: 29155844] (WB, Human)
• Ho JC, Abdullah LN, Pang QY et al. Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway PLoS ONE. 2017 Nov 16 [PMID: 29145444] (WB, Human)
• Sodi VL, Bacigalupa ZA, Ferrer CM et al. Nutrient sensor O-GlcNAc transferase controls cancer lipid metabolism via SREBP-1 regulation. Oncogene. 2017 Oct 23 [PMID: 29059153] (WB, Human)
• Strowitzki MJ, Ritter AS, Radhakrishnan P et al. Pharmacological HIF-inhibition attenuates postoperative adhesion formation. Sci Rep. 2017 Oct 13 [PMID: 29030625] (WB, Mouse)
  
  Details:
  HIF1 alpha antibody used to show the effect on cells of YC-1 exposure in both normoxic and hypoxic by western blot in mouseNIH/3T3 cells
• Sharma B, Ho L, Ford GH et al. Alternative Progenitor Cells Compensate to Rebuild the Coronary Vasculature in Elabela- and Apj-Deficient Hearts Dev. Cell 2017 Sep 25 [PMID: 28890073] (IHC, Mouse)
• Huang R, Hayashi Y, Yan X et al. HIF1A is a critical downstream mediator for hemophagocytic lymphohistiocytosis. Haematologica. 2017 Aug 31 [PMID: 28860338] (Mouse)
  
  Details:
  This citation used the DyLight 405 form of this antibody.
• Johansson E, Grassi ES, Pantazopoulou V et al. CD44 Interacts with HIF-2alpha to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells Cell Rep 2017 Aug 15 [PMID: 28813675] (WB, Human)
• Sun W, Kato H, Kitajima S et al. Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression. Sci Rep 2017 Aug 03 [PMID: 28775317] (WB, Human)
• Nakashima R, Goto Y, Koyasu S et al. UCHL1-HIF-1 axis-mediated antioxidant property of cancer cells as a therapeutic target for radiosensitization Sci Rep 2017 Jul 31 [PMID: 28761052] (WB, Human)
• Braverman J, Stanley SA. Nitric Oxide Modulates Macrophage Responses to Mycobacterium tuberculosis Infection through Activation of HIF-1alpha and Repression of NF-kB J. Immunol. 2017 Jul 28 [PMID: 28754681] (WB)
• Cosin-Roger J, Simmen S, Melhem H et al. Hypoxia ameliorates intestinal inflammation through NLRP3/mTOR downregulation and autophagy activation Nat Commun 2017 Jul 24 [PMID: 28740109] (WB, Human)
• Casciello F, Al-Ejeh F, Kelly G et al. G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis. Proc. Natl. Acad. Sci. U.S.A. 2017 Jun 19 [PMID: 28630300] (WB, Human)
• Zheng X, Narayanan S, Zheng X et al. A Notch-independent mechanism contributes to the induction of Hes1 gene expression in response to hypoxia in P19 cells. Exp. Cell Res. 2017 Jun 09 [PMID: 28602625] (IP, WB, Mouse)
• Licarete E, Sesarman A, Rauca VF et al. HIF-1a acts as a molecular target for simvastatin cytotoxicity in B16.F10 melanoma cells cultured under chemically induced hypoxia. Oncol Lett May 1 2017 [PMID: 28521491] (WB, Mouse)
• Kiriakidis S, Hoer SS, Burrows N et al. Complement C1q is hydroxylated by collagen prolyl 4 hydroxylase and is sensitive to off-target inhibition by prolyl hydroxylase domain inhibitors that stabilize hypoxia-inducible factor. Kidney Int. May 12 2017 [PMID: 28506759] (IB, Mouse)
• Jackson CS, Durandt C, Janse van Rensburg I et al. Targeting the aryl hydrocarbon receptor nuclear translocator complex with DMOG and Stemregenin 1 improves primitive hematopoietic stem cell expansion. Stem Cell Res. Apr 20 2017 [PMID: 28445828]
• Tokumoto Y, Tamaki S, Kabe Y et al. Quiescence of adult oligodendrocyte precursor cells requires thyroid hormone and hypoxia to activate Runx1. Sci Rep Apr 21 2017 [PMID: 28432293] (ICC/IF, Rat)
• Duan C, Perez-Torres CJ, Yuan L et al. Can anti-vascular endothelial growth factor antibody reverse radiation necrosis. A preclinical investigation. J. Neurooncol. Apr 19 2017 [PMID: 28425047]
• Schuoler C, Haider TJ, Leuenberger C et al. Aquaporin 1 controls the functional phenotype of pulmonary smooth muscle cells in hypoxia-induced pulmonary hypertension. Basic Res. Cardiol. May 1 2017 [PMID: 28409279] (WB, Mouse)
• Hum JM, Clinkenbeard EL, Ip C et al. The metabolic bone disease associated with the Hyp mutation is independent of osteoblastic HIF1a expression. Bone Reports. Jan 17 2017 [PMID: 28377980] (WB, Rat)
• Sato Y, Tsuyama T, Sato C et al. Hypoxia reduces HNF4alpha/MODY1 protein expression in pancreatic B-cells by activating AMP-activated protein kinase. J. Biol. Chem. Mar 31 2017 [PMID: 28364040] (WB, Mouse)
• Landfried B, Samardzija M, Barben M et al. Digoxin-induced retinal degeneration depends on rhodopsin. Cell Death Dis. Mar 16 2017 [PMID: 28300845] (WB, Mouse)
• Wang S, Zeng H, Chen ST et al. Ablation of endothelial prolyl hydroxylase domain protein-2 promotes renal vascular remodelling and fibrosis in mice. J. Cell. Mol. Med. Mar 7 2017 [PMID: 28266128] (WB, Mouse)
• Maturu P, Jones D, Ruteshouser EC et al. Role of Cyclooxygenase-2 Pathway in Creating an Immunosuppressive Microenvironment and in Initiation and Progression of Wilms' Tumor. Neoplasia. Mar 1 2017 [PMID: 28254151] (IHC-P, Mouse)
• Brock M, Schuoler C, Leuenberger C et al. Analysis of hypoxia-induced noncoding RNAs reveals metastasis-associated lung adenocarcinoma transcript 1 as an important regulator of vascular smooth muscle cell proliferation Exp. Biol. Med. (Maywood) Jan 1 2017 [PMID: 28056547] (Mouse)
• Razafinjatovo CF, Stiehl D, Deininger E et al. VHL missense mutations in the p53 binding domain show different effects on p53 signaling and HIFalpha degradation in clear cell renal cell carcinoma. Oncotarget. Feb 7 2017 [PMID: 28052007] (WB, Mouse)
• Negrette-Guzman M, Huerta-Yepez S, Vega MI et al. Sulforaphane induces differential modulation of mitochondrial biogenesis and dynamics in normal cells and tumor cells. Food Chem. Toxicol. Dec 18 2016 [PMID: 27993529] (ICC/IF, Human)
• Myllymaki MN, Maatta J, Dimova EY et al. Notch downregulation and extramedullary erythrocytosis in HIF prolyl 4-hydroxylase-2-deficient mice. Mol. Cell. Biol. Nov 7 2016 [PMID: 27821476]
• Morita M, Sato Y, Iwasaki R et al. Selective Estrogen Receptor Modulators Suppress Hif1alpha Protein Accumulation in Mouse Osteoclasts. PLoS ONE. Nov 1 2016 [PMID: 27802325] (WB, Moues)
• Hoogewijs D, Vogler M, Zwenger E et al. Oxygen-dependent regulation of aquaporin-3 expression. Hypoxia (Auckl). Nov 1 2016 [PMID: 27800511] (Mouse)
• Guentsch A, Beneke A, Swain L et al. PHD2 Is a Regulator for Glycolytic Reprogramming in Macrophages. Mol. Cell. Biol. Jan 1 2017 [PMID: 27795296]
• Tian X, Nguyen M, Foote HP et al. CRLX101, a nanoparticle-drug conjugate containing camptothecin, improves rectal cancer chemoradiotherapy by inhibiting DNA repair and HIF-1alpha. Cancer Res. Oct 26 2016 [PMID: 27784746]
• Ostergaard L, Rudiger A, Wellmann S et al. Arginine-vasopressin marker copeptin is a sensitive plasma surrogate of hypoxic exposure. Hypoxia (Auckl) 2014 Sep 11 [PMID: 27774473]
• Sousa LM, Silva RD, Fonseca VU et al. Is the canine corpus luteum an insulin-sensitive tissue?. J. Endocrinol. Dec 1 2016 [PMID: 27679426]
• Clever D, Roychoudhuri R, Constantinides MG et al. Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche. Cell. Aug 25 2016 [PMID: 27565342]
• Koivunen P, Fell SM, Lu W et al. The 2-oxoglutarate analog 3-oxoglutarate decreases normoxic hypoxia-inducible factor-1alpha in cancer cells, induces cell death, and reduces tumor xenograft growth. Hypoxia (Auckl) 2016 Mar 04 [PMID: 27525289]
• Pandya PH, Fisher AJ, Mickler EA et al. HIF-1alpha Regulates CD55 in Airway Epithelium. Am J Respir Cell Mol Biol 2016 Aug 5 [PMID: 27494303]
• Guimaraes TA, Farias LC, Santos ES et al. Metformin increases PDH and suppresses HIF-1alpha under hypoxic conditions and induces cell death in oral squamous cell carcinoma. Oncotarget. Jul 26 2016 [PMID: 27474170] (WB, IHC, Human)
• Jung GS, Jeon JH, Choi YK et al. Pyruvate dehydrogenase kinase regulates hepatitis C virus replication. Sci Rep. Jul 29 2016 [PMID: 27471054] (IHC, WB, Human)
• Leinonen H, Rossi M, Salo AM et al. Lack of P4H-TM in mice results in age-related retinal and renal alterations. Hum. Mol. Genet. Jul 27 2016 [PMID: 27466183] (WB, Mouse)
• Xue H, Chen D, Zhong YK et al. Deferoxamine ameliorates hepatosteatosis via several mechanisms in ob/ob mice Ann. N. Y. Acad. Sci. 2016 Jul 01 [PMID: 27447538] (WB, Mouse)
• Braverman J, Sogi KM, Benjamin D et al. HIF-1 alpha Is an Essential Mediator of IFN-gamma-Dependent Immunity to Mycobacterium tuberculosis J. Immunol. 2016 Jul 18 [PMID: 27430718]
• Luo B, Wang J, Liu Z et al. Phagocyte respiratory burst activates macrophage erythropoietin signalling to promote acute inflammation resolution Nat Commun 2016 Jul 11 [PMID: 27397585] (WB)
• Nunomiya A, Shin J, Kitajima Y et al. Activation of the hypoxia-inducible factor pathway induced by prolyl hydroxylase domain 2 deficiency enhances the effect of running training in mice Acta Physiol (Oxf) 2016 Jul 09 [PMID: 27393382] (WB, Mouse)
• Diaz-Trelles R, Scimia MC, Bushway P et al. Notch-independent RBPJ controls angiogenesis in the adult heart. Nat Commun 2016 Jun 30 [PMID: 27357444] (WB)
• Muinos-Lopez E, Ripalda-Cemborain P, Lopez-Martinez T et al. Hypoxia and Reactive Oxygen Species Homeostasis in Mesenchymal Progenitor Cells Define a Molecular Mechanism for Fracture Nonunion. Stem Cells Jun 2 2016 [PMID: 27250101]
• Bullen JW, Tchernyshyov I, Holewinski RJ et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016 May 31 [PMID: 27245613] (WB, Human)
• Halt KJ, Parssinen HE, Junttila SM, Saarela U. CD146+ cells are essential for kidney vasculature development. Kidney International. 2016 Apr 18 [PMID: 27165833] (WB, Mouse)
• Christinat Y, Pawlowski R, Krek W. jSplice: a high-performance method for accurate prediction of alternative splicing events and its application to large-scale renal cancer transcriptome data. Bioinformatics 2016 Jul 15 [PMID: 27153587]
• Ruffenach G, Chabot S, Tanguay VF et al. Role for RUNX2 in Proliferative and Calcified Vascular Lesions in Pulmonary Arterial Hypertension. Am. J. Respir. Crit. Care Med. May 5 2016 [PMID: 27149112] (IHC-P, WB, IP, Rat, Human)
• Takahashi N, Davy PM, Gardner LH et al. Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle. PLoS ONE. May 6 2016 [PMID: 27148974] (IHC-WhMt, ICC/IF, IHC-P, Mouse)
• Msaki A, Pasto A, Curtarello M et al. A hypoxic signature marks tumors formed by disseminated tumor cells in the BALB-neuT mammary cancer model. Oncotarget. May 31 2016 [PMID: 27105499] (WB, Mouse)
• Trinh NT, Yamashita T, Ohneda K et al. Increased Expression of EGR-1 in Diabetic Human Adipose Tissue-Derived Mesenchymal Stem Cells Reduces Their Wound Healing Capacity. Stem Cells Dev 2016 May 15 [PMID: 26988763]
• Shin J, Nunomiya A, Kitajima Y et al. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle 2016 Mar 05 [PMID: 26949511] (WB)
• Ruf M, Moch H, Schraml P. PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma. Int J Cancer 2016 Jul 15 [PMID: 26945902]
• Zhou F, Dai A, Jiang Y et al. SENP1 enhances hypoxiainduced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxiainducible factor1alpha. Mol Med Rep 2016 Apr [PMID: 26935971] (WB)
• Regan Anderson TM, Ma SH, Raj GV et al. Breast Tumor Kinase (Brk/PTK6) Is Induced by HIF, Glucocorticoid Receptor, and PELP1-Mediated Stress Signaling in Triple-Negative Breast Cancer Cancer Res. Jan 29 2016 [PMID: 26825173] (WB, Human)
• Lee SD, Kim W, Jeong JW et al. AK-1, a SIRT2 inhibitor, destabilizes HIF-1alpha and diminishes its transcriptional activity during hypoxia. Cancer Lett. 2016 Jan 22 [PMID: 26808575] (ICC/IF, Human)
  
  Details:
  HIF1 alpha antibody was used for immunofluorescence/ICC-IF staining of A549 cells which were exposed to hypoxia for six hours in the presence of a SIRT2-specific inhibitor (10uM AK-1 ).
• Qian F, He M, Duan W et al. Cross regulation between hypoxia-inducible transcription factor-1alpha (HIF-1alpha) and transforming growth factor (TGF)-ss1 mediates nickel oxide nanoparticles (NiONPs)-induced pulmonary fibrosis. Am J Transl Res 2015 [PMID: 26807184] (WB)
• Tando T, Sato Y, Miyamoto K et al. Hif1alpha is required for osteoclast activation and bone loss in male osteoporosis. Biochem. Biophys. Res. Commun. 2016 Feb 05 [PMID: 26792721] (WB, IHC-P, Mouse)
  
  Details:
  HIF1 alpha antibody was used for IHC-P analysis of femurs from orchidectomy/ORX or sham-operated Wild type C57BL/6 mice - the bones fixed 10% formalin, decalcified in 8% Na2EDTA, antigen was retrieved for 8 minutes at RT with 0.4 mg/mL Proteinase K and primary detected using Alexa488-conjugated goat anti-rabbit secondary (Fig. 1). The antibody was also used for WB on lysates of Raw264.7 cells cultured with or without testosterone in normoxic or hypoxic conditions.
• Kinoshita T, Fujii H, Hayashi Y et al. Prognostic significance of hypoxic PET using (18)F-FAZA and (62)Cu-ATSM in non-small-cell lung cancer. Lung Cancer 2016 Jan [PMID: 26711935]
• Laukka T, Mariani CJ, Ihantola T et al. Fumarate and Succinate Regulate Expression of Hypoxia-inducible Genes via TET Enzymes. J Biol Chem 2016 Feb 19 [PMID: 26703470]
• Aquino-Galvez A, Gonzalez-avila G. Effects of 2-methoxyestradiol on apoptosis and HIF-1a and HIF-2a expression in lung cancer cells under normoxia and hypoxia. ONCOLOGY REPORTS. 2015 Aug 20 [PMID: 26548300] (WB, ICC/IF, Human)
• Zhang J, Wang C, Chen X et al. EglN2 associates with the NRF1-PGC1alpha complex and controls mitochondrial function in breast cancer. EMBO J 2015 Dec 02 [PMID: 26492917] (WB, Human)
• Karsikas S, Myllymaki M, Heikkila M et al. HIF-P4H-2 deficiency protects against skeletal muscle ischemia-reperfusion injury. J Mol Med (Berl) 2016 Mar [PMID: 26452676]
• Saini Y, Proper SP, Dornbos P et al. Loss of Hif-2alpha Rescues the Hif-1alpha Deletion Phenotype of Neonatal Respiratory Distress In Mice. PLoS One 2015 [PMID: 26422241] (IHC, Mouse)
• Zeng Z, Huang WD, Gao Q et al. Arnebin-1 promotes angiogenesis by inducing eNOS, VEGF and HIF-1a expression through the PI3K-dependent pathway Int. J. Mol. Med. 2015 Jul 22 [PMID: 26202335] (WB, Human)
• Chen X, Liu J, He B et al. Vascular endothelial growth factor (VEGF) regulation by hypoxia inducible factor-1 alpha (HIF1A) starts and peaks during endometrial breakdown, not repair, in a mouse menstrual-like model Hum. Reprod. 2015 Jun 25 [PMID: 26113661] (IHC-P, Mouse)
  
  Details:
  HIF1 alpha antibody was used at 1:100 dilution for IHC-P analysis of 5um paraffin sections of uteri tissues from mouse menstrual-like model (details in Fan et al Faseb J 2008 22 3571- 3580).
• Liu J, You P, Chen G et al. Hyperactivated FRS2a-mediated signaling in prostate cancer cells promotes tumor angiogenesis and predicts poor clinical outcome of patients Oncogene. 2015 Jun 22 [PMID: 26096936] (WB, Human)
• Mirtschink P, Krishnan J, Grimm F et al. HIF-driven SF3B1 induces KHK-C to enforce fructolysis and heart disease Nature. 2015 Jun 25 [PMID: 26083752] (WB, Mouse)
• Guo C, Zhang Y, Wang T, Zhong M. Intranasal deferoxamine attenuates synapse loss via up?regulating the P38/HIF?1alpha pathway on the brain of APP/PS1 transgenic mice. Frontiers in Aging Neuroscience. 2015 Jun 02 [PMID: 26082716] (IHC-P, WB, Mouse)
• Zhao H, Wu Y, Chen Y, Liu H. Clinical significance of hypoxia-inducible factor 1 and VEGF-A in osteosarcoma Int. J. Clin. Oncol. 2015 Jun 16 [PMID: 26077139] (IHC-P, WB, Human)
• Xie L, Pi X, Townley-Tilson WH et al. PHD2/3-dependent hydroxylation tunes cardiac response to beta-adrenergic stress via phospholamban. J Clin Invest 2015 Jul 1 [PMID: 26075818] (WB)
• Yorulmaz H, Ozkok E, Erguven M et al. Effect of simvastatin on mitochondrial enzyme activities, ghrelin, hypoxia-inducible factor 1alpha in hepatic tissue during early phase of sepsis. Int J Clin Exp Med 2015 [PMID: 26064259] (IHC, Rat)
• Zieseniss A, Hesse AR, Jatho A et al. Cardiomyocyte-Specific Transgenic Expression of Prolyl-4-Hydroxylase Domain 3 Impairs the Myocardial Response to Ischemia. Cell Physiol Biochem 2015 May 27 [PMID: 26044310]
• Hwang HJ, Lynn SG, Vengellur A et al. Hypoxia Inducible Factors Modulate Mitochondrial Oxygen Consumption and Transcriptional Regulation of Nuclear-Encoded Electron Transport Chain Genes. Biochemistry 2015 Jun 23 [PMID: 26030260]
• Valbuena GN, Rizzardini M, Cimini S et al. Metabolomic Analysis Reveals Increased Aerobic Glycolysis and Amino Acid Deficit in a Cellular Model of Amyotrophic Lateral Sclerosis. Mol. Neurobiol. 2015 May 12 [PMID: 25963727] (WB, Human, Mouse)
• Conley SJ, Baker TL, Burnett JP et al. CRLX101, an investigational camptothecin-containing nanoparticle-drug conjugate, targets cancer stem cells and impedes resistance to antiangiogenic therapy in mouse models of breast cancer Breast Cancer Res. Treat. 2015 Apr 01 [PMID: 25833208] (WB, IHC, Human)
• Tan SC, Gomes RS, Yeoh KK et al. Preconditioning of cardiosphere-derived cells with hypoxia or prolyl-4-hydroxylase inhibitors increases stemness and decreases reliance on oxidative metabolism. Cell Transplant. 2015 Mar 06 [PMID: 25751158] (WB, Rat)
• Albers J, Danzer C, Rechsteiner M et al. A versatile modular vector system for rapid combinatorial mammalian genetics. J Clin Invest 2015 Apr [PMID: 25751063] (WB)
• Lee M, Choi SY, Kang BH et al. Differential microscopic finding and glucose transporter 3 expression in terminal chorionic villi among birth weight-discordant twin placentas Histol. Histopathol. 2015 Mar 02 [PMID: 25728965] (IHC-P)
• Kato H, Izumi K, Uenoyama A et al. Hypoxia Induces an Undifferentiated Phenotype of Oral Keratinocytes in vitro Cells Tissues Organs (Print). 2015 Feb 18 [PMID: 25720390]
• Qiao N, Xu C, Zhu YX et al. Ets-1 as an early response gene against hypoxia-induced apoptosis in pancreatic beta-cells Cell Death Dis. 2015 Feb 19 [PMID: 25695603]
• Stantic M, Sakil HA, Zirath H et al. TAp73 suppresses tumor angiogenesis through repression of proangiogenic cytokines and HIF-1alpha activity.. Proc Natl Acad Sci U S A. 2015 Jan 6 [PMID: 25535357] (WB,ChIP)
• Sato Yoshifumi, Inoue Masahiro, Yoshizawa Tatsuya, Yamagata Kazuya. Moderate hypoxia induces beta-cell dysfunction with HIF-1-independent gene expression changes. PLoS One. 2014 Dec 12 [PMID: 25503986] (WB)
• Takamiya R, Takahashi M, Uehara Y, Ariki S. The single N-glycan deletion mutant of soluble ErbB3 protein attenuates heregulinb1-induced tumor progression by blocking of the HIF-1 and Nrf2 pathway. Biochem Biophys Res Commun. 2014 Oct 24 [PMID: 25451255] (WB, Human)
  
  Details:
  HIF1 alpha antibody used for WB on experiments involving MCF7, T47D and BT474 cells
• Fine Jm, Renner Db, Forsberg Ac et al. Intranasal deferoxamine engages multiple pathways to decrease memory loss in the APP/PS1 model of amyloid accumulation. Neurosci. Lett. 2014 Nov 13 [PMID: 25445365] (WB, Mouse)
• Xie Yuan, Zhang Jin, Lin Ying et al. Defining the role of oxygen tension in human neural progenitor fate. Stem Cell Reports. 2014 Nov 11 [PMID: 25418722] (ICC/IF, Human)
• Lam Gc, Sefton Mv. Tuning Graft- and Host-Derived Vascularization in Modular Tissue Constructs: A Potential Role of HIF1 Activation Tissue Eng Part A et al. 2014 Nov 07 [PMID: 25379774] (IHC-P, Human)
  
  Details:
  HIF1 alpha antibody used for IHC-P on human modular tissues /sub-millimeter collagen modules containing adipose-derived mesenchymal stromal cells (adMSC) and enveloped by human umbilical vein endothelial cells (HUVEC) subcutaneously implanted in severe-combined immunodeficient mice with a beige-mutation (SCID-bg) mice - 24 hour fixation in 10% neutral buffered formalin, 4um paraffin sections, 1:2000 primary dilution,
• Clarke Rh, Moosa S, Anzivino M et al. Sustained radiosensitization of hypoxic glioma cells after oxygen pretreatment in an animal model of glioblastoma and in vitro models of tumor hypoxia. PLoS OnE. 2014 Oct 29 [PMID: 25350400] (WB, Human)
• Takahashi H, Inoue A, Kawabe Y et al. Oct-3/4 promotes tumor angiogenesis through VEGF production in glioblastoma Brain Tumor Pathol et al. 2014 Oct 28 [PMID: 25348671] (WB, ICC/IF, Human)
  
  Details:
  HIF1 alpha antibody used for WB and ICC-IF on U251/EGFP or U251/EGFP-Oct-3/4 cells (Fig 5). WB also performed on cells subjected to Oct-3/4 knockdown (Fig 6b) and U251/EGFP-Oct-3/4 cells were treated with AKT inhibitor (Fig 6c).
• Burgi S, Seuwen A, Keist R et al. In Vivo Imaging of Hypoxia-Inducible Factor Regulation in a Subcutaneous and Orthotopic GL261 Glioma Tumor Model Using a Reporter Gene Assay. Mol Imaging. 2014 Sep 01 [PMID: 25248521]
• Okon IS, Coughlan KA, Zhang C et al. Protein kinase LKB1 promotes RAB7-mediated neuropilin-1 degradation to inhibit angiogenesis. J Clin. Invest. 2014 Sep 02 [PMID: 25180605] (WB, IP, Human)
• Huang BW, Miyazawa M, Tsuji Y et al. Distinct Regulatory Mechanisms of the Human Ferritin Gene by Hypoxia and Hypoxia Mimetic Cobalt Chloride at the Transcriptional and Post-transcriptional Levels. Cell. Signal. 2014 Aug 26 [PMID: 25172425] (WB, Human)
• Hesse AR, Levent E, Zieseniss A et al. Lights on for HIF-1a: Genetically Enhanced Mouse Cardiomyocytes for Heart Tissue Imaging. Cell. Physiol. Biochem. 2014 Jul 30 [PMID: 25095893] (WB, Mouse)
  
  Details:
  HIF-1 alpha antibody used for WB on protein extracts of hypoxia exposed ODD-Luc-CMs/Cardiomyocytes derived from an embryonic stem cell (ESC) lines (ODD-Luc ESCs) that was generated from a Tg ROSA26 ODD-Luc/+ mouse (Figure 2D).
• Kaasinen E, Rahikkala E, Koivunen P et al. Clinical characterization, genetic mapping and whole-genome sequence analysis of a novel autosomal recessive intellectual disability syndrome. Eur J Med Genet 2014 Jul 28 [PMID: 25078763] (WB, Human)
  
  Details:
  HIF1a/HIF-1 alpha antibody used for WB on proteins extracts from human lymphoblasts cultured under normoxic and hypoxic conditions. Lymphoblasts were from six patients who developed an unexplained syndromic form of profound intellectual disability (Supplementary Fig. 2b and c).
• Ueda J, Ho JC, Lee KL et al. The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. Mol. Cell. Biol. 2014 Jul 28 [PMID: 25071150] (WB, Mouse)
  
  Details:
  HIF1 alpha antibody used in WB on 1% hypoxia exposed wild-type murine embryonic stem cells (Fig 4A - HIF1a used as positive control for hypoxic induction).
• Abu-Remaileh M, Aqeilan RI. Tumor suppressor WWOX regulates glucose metabolism via HIF1alpha modulation. Cell Death Differ 2014 Nov [PMID: 25012504] (WB)
• Slominski A, Kim TK, Brozyna AA et al. The role of melanogenesis in regulation of melanoma behavior: Melanogenesis leads to stimulation of HIF-1a expression and HIF-dependent attendant pathways. Arch. Biochem. Biophys. 2014 Jul 02 [PMID: 24997364] (WB, Human, Hamster)
  
  Details:
  HIF-1 alpha antibody used for WB on whole cell lysates of Bomirski hamster AbC1 and human SKMEL-188 stable lined (amelanotic /non-pigmented and melanotic /pigmented lines) with Hif1a wild type/WT and knockout /KO mammary tumor epithelial cells (MTECs) derived from the polyoma virus middle T transgenic mouse (MMTV-PyMT) as positive control for HIF-1 alpha. Antibody used at 1:5000 dilution and the data is shown in Fig 1.
• Kono K, Tamashiro DA, Alarcon VB. Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of hippo signaling in the mouse blastocyst. Dev. Biol. 2014 Jul 02 [PMID: 24997360] (WB, Human, Hamster)
  
  Details:
  HIF-2 alpha antibody used for WB on lysates of Bomirski hamster AbC1 and human SKMEL-188 cell lines (Fig. 1).
• Zheng Xingnan, Zhai Bo, Koivunen Peppi et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014 Jul 01 [PMID: 24990963] (WB, Mouse)
• Chen RL, Ogunshola OO, Yeoh KK et al. HIF prolyl hydroxylase inhibition prior to transient focal cerebral ischaemia is neuroprotective in mice J. Neurochem. 2014 Jun 26 [PMID: 24974727] (IHC-P, WB, Mouse, Rat)
  
  Details:
  HIF-1 alpha antibody used for IHC-P on FFPE tissue sections of C57/B6 mouse brains (in vivo transient focal cerebral ischaemia model) treated or not with 2-(1-chloro-4-hydroxyisoquinoline-3-carboxamido) acetic acid (IOX3), a selective small molecule inhibitor of hypoxia-inducible factor (HIF) prolyl hydroxylases (staining images - Figure 4). Antibody also used for WB on rat brain endothelial cell line RBE4 exposed to IOX3 and hypoxia (Figure 6)
• Peng YJ, Yuan G, Khan S et al. Regulation of Hypoxia Inducible Factor-alpha Isoforms and Redox State By Carotid Body Neural Activity in Rats. J. Physiol. (Lond.). 2014 Jun 27 [PMID: 24973414] (IHC-Fr, WB, Rat)
• Mangiavini L, Merceron C, Araldi E et al. Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development Dev. Biol. 2014 Jun 24 [PMID: 24972088] (WB, Mouse)
  
  Details:
  HIF-1 alpha antibody used for Western blot at 1:1000 dilution in protein lysate from chondrocytes of CNTRL and VHL mice ( protocol details provided in this article, WB image - Supplemental Fig. 1, Panel B)
• Rahtu-Korpela Lea, Karsikas Sara, Horkko Sohvi et al. HIF prolyl 4-hydroxylase-2 inhibition improves glucose and lipid metabolism and protects against obesity and metabolic dysfunction. Diabetes. 2014 May 01 [PMID: 24789921] (Mouse)
• Yu Z, Ge Y, Xie L et al. Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1alpha in HepG2 cells. Cell. Signal. 2014 Mar 29 [PMID: 24686083] (WB, IP, Human)
• Chen X, Iliopoulos D, Zhang Q et al. XBP1 promotes triple-negative breast cancer by controlling the HIF1a pathway. Nature 4/3/2014 [PMID: 24670641] (IP, Human)
• Mochizuki A, Pace A, Rockwell CE et al. Hepatic Stellate Cells Orchestrate Clearance of Necrotic Cells in a Hypoxia-Inducible Factor-1a-Dependent Manner by Modulating Macrophage Phenotype in Mice. J. Immunol. 3/17/2014 [PMID: 24639359] (IHC-Fr, Mouse)
• Swain Lija, Wottawa Marieke, Hillemann Annette et al. Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions. J Leukoc Biol. 2014 Mar 13 [PMID: 24626957] (Mouse)
• Ekman Mari, Uvelius Bengt, Albinsson Sebastian, Sward Karl. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest. 2014 May 01 [PMID: 24589856] (WB, Rat)
• Yang EJ, Lee J, Lee SY et al. EGCG Attenuates Autoimmune Arthritis by Inhibition of STAT3 and HIF-1 alpha with Th17/Treg Control. PLoS ONE 2014 Feb 21 [PMID: 24558360] (WB, IHC-P, Mouse)
• Van Hung Tran, Emoto Noriaki, Vignon-Zellweger Nicolas et al. Inhibition of vascular endothelial growth factor receptor under hypoxia causes severe, human-like pulmonary arterial hypertension in mice: potential roles of interleukin-6 and endothelin. Life Sci. 2014 Nov 24 [PMID: 24412382]
• Hopfner U, Schenck TL, Chavez MN et al. Development of photosynthetic biomaterials for in vitro tissue engineering. Acta Biomater 2014 Jan 7 [PMID: 24406198] (ICC/IF, Mouse)
• Hell MP, Duda M, Weber TC et al. Ischemic renal injury reveals in vivo functions for the von Hippel-Lindau tumor suppressor in mitotic fidelity control. Cancer Res 2013 Dec 20 [PMID: 24362914] (ICC/IF, IHC-P, Mouse)
• Zhu J, Jiang X, Chehab FF. FoxO4 Interacts with the Sterol Regulatory Factor SREBP2 and the Hypoxia Inducible Factor HIF2A at the CYP51 Promoter. J Lipid Res 2013 Dec 18 [PMID: 24353279] (WB, Mouse)
• Xuan Y, Hur H, Ham IH et al. Dichloroacetate attenuates hypoxia-induced resistance to 5-fluorouracil in gastric cancer through the regulation of glucose metabolism. Exp Cell Res 2013 Dec 14 [PMID: 24342832] (WB, IHC-P, Human)
• Klug F, Prakash H, Huber PE et al. Low-dose irradiation programs macrophage differentiation to an iNOS+/M1 phenotype that orchestrates effective T cell immunotherapy. Cancer Cell. 2013 Nov 11 [PMID: 24209604] (WB, IP, Mouse)
• De A, De A, Papasian C et al. Emblica officinalis Extract Induces Autophagy and Inhibits Human Ovarian Cancer Cell Proliferation, Angiogenesis, Growth of Mouse Xenograft Tumors. PLoS One. 2013 Aug 15 [PMID: 24133573] (IHC-P, Human)
• Wiesner D, Merdian I, Lewerenz J et al. Fumaric Acid Esters Stimulate Astrocytic VEGF Expression through HIF-1alpha and Nrf2. PLoS One. 2013 Oct 3 [PMID: 24098549] (WB, Mouse)
• Lijkwan M, Hellingman A, Bos E et al. Short Hairpin RNA Gene Silencing of Prolyl Hydroxylase-2 with a Minicircle Vector Improves Neovascularization of Hindlimb Ischemia. Hum Gene Ther. 2013 Oct 3 [PMID: 24090375]
• Cowburn AS, Alexander LE, Southwood M et al. Epidermal Deletion of HIF-2alpha Stimulates Wound Closure. J Invest Dermatol. 2013 Sep 13 [PMID: 24037341] (WB, Human)
• Weissmuller T, Glover LE, Fennimore B et al. HIF-dependent regulation of AKAP12 (gravin) in the control of human vascular endothelial function. FASEB J 2014 Jan [PMID: 24029533] (Human)
• Puri S, Garcia-Nunez A, Hebrok M, Cano DA. Elimination of Von Hippel-Lindau Function Perturbs Pancreas Endocrine Homeostasis in Mice. PLoS One. 2013 Aug 20 [PMID: 23977255] (IHC, ICC/IF, Mouse)
• Regan Anderson TM, Peacock DL, Daniel AR et al. Breast Tumor Kinase (Brk/PTK6) Is a Mediator of Hypoxia-Associated Breast Cancer Progression. Cancer Res. 2013 Sep 15 [PMID: 23928995] (WB, Human)
• Yang B, Janardhanan R, Vohra P et al. Adventitial transduction of lentivirus-shRNA-VEGF-A in arteriovenous fistula reduces venous stenosis formation. Kidney Int. 2013 Aug 7 [PMID: 23924957] (IHC-P, Mouse)
• Nabatov AA, Hatzis P, Rouschop KM et al. Hypoxia inducible NOD2 interacts with 3-O-sulfogalactoceramide and regulates vesicular homeostasis. Biochim Biophys Acta 2013 Jul 21 [PMID: 23880069] (ChIP, Human)
• Vogler M, Vogel S, Krull S et al. Hypoxia Modulates Fibroblastic Architecture, Adhesion and Migration: A Role for HIF-1alpha in Cofilin Regulation and Cytoplasmic Actin Distribution. PLoS One 2013 Jul 18 [PMID: 23874890] (WB, Mouse)
• Wu J, Chen P, Li Y et al. HIF-1alpha in heart: Protective mechanisms. Am J Physiol Heart Circ Physiol. 2013 Sep [PMID: 23873797] (WB, Mouse)
• Kopriva SE, Chiasson VL, Mitchell BM, Chatterjee P. TLR3-Induced Placental miR-210 Down-Regulates the STAT6/Interleukin-4 Pathway. PLoS One 2013 Jul 2 [PMID: 23844087] (WB, Mouse)
• Jones LW, Fels DR, West M et al. Modulation of Circulating Angiogenic Factors and Tumor Biology by Aerobic Training in Breast Cancer Patients Receiving Neoadjuvant Chemotherapy. Cancer Prev Res (Phila) 2013 Jul 10 [PMID: 23842792] (IHC-P, Human)
• Ikeda J, Ichiki T, Matsuura H et al. Deletion of phd2 in myeloid lineage attenuates hypertensive cardiovascular remodeling. J Am Heart Assoc 2013 Jun 18 [PMID: 23778187] (WB, Human)
• Kerkela R, Karsikas S, Szabo Z et al. Activation of hypoxia response in endothelial cells contributes to ischemic cardioprotection. Mol Cell Biol. 2013 Aug [PMID: 23775121] (WB, Mouse)
• Nakayama K. CREB and NF-kB are activated during prolonged hypoxia and cooperatively regulate the induction of matrix metalloproteinase MMP1. J Biol Chem 2013 Jun 17 [PMID: 23775082] (WB, Human)
• Menezes JP, Almeida TF, Petersen ALet al. Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major. Microbes Infect 2013 Apr 26 [PMID: 23628411] (ICC/IF, Mouse)
• Luo B, Jiang M, Yang X et al. Erythropoietin is a hypoxia inducible factor-induced protective molecule in experimental autoimmune neuritis. Biochim Biophys Acta 2013 Aug [PMID: 23603807] (WB, Rat)
• Pietkiewicz S, Sohn D, Piekorz RP et al. Oppositional Regulation of Noxa by JNK1 and JNK2 during Apoptosis Induced by Proteasomal Inhibitors. PLoS One 2013 Apr 11 [PMID: 23593480] (WB, Human)
• Nagai J, Yamamoto A, Yumoto R, Takano M. Albumin overload induces expression of hypoxia-inducible factor 1alpha and its target genes in HK-2 Human renal proximal tubular cell line. Biochem Biophys Res Commun 2013 Apr 12 [PMID: 23587905] (WB, Human)
• Hanna SC, Krishnan B, Bailey ST et al. HIF1alpha and HIF2alpha independently activate SRC to promote melanoma metastases. J Clin Invest 2013 May 1 [PMID: 23563312] (IHC-P, Human)
• Takeuchi-Yorimoto A, Noto T, Yamada A et al. Persistent fibrosis in the liver of choline-deficient and iron-supplemented l-amino acid-defined diet-induced nonalcoholic steatohepatitis rat due to continuing oxidative stress after choline supplementation. Toxicol Appl Pharmacol 2013 Feb 26 [PMID: 23481610] (WB, Rat)
• Karuppagounder SS, Basso M, Sleiman SF et al. In vitro ischemia suppresses hypoxic induction of hypoxia-inducible factor-1alpha by inhibition of synthesis and not enhanced degradation J Neurosci Res 2013 Mar 4 [PMID: 23456821] (WB, Human)
• Tao J, Doughman Y, Yang K et al. Epicardial HIF signaling regulates vascular precursor cell invasion into the myocardium. Dev Biol 2013 Feb 2 [PMID: 23384563] (WB, Mouse)
• Kaufmann MR, Barth S, Konietzko U et al. Dysregulation of Hypoxia-Inducible Factor by Presenilin/gamma-Secretase Loss-of-Function Mutations. J Neurosci 2013 Jan 30 [PMID: 23365231] (WB, Mouse, Human)
• Tsapournioti S, Mylonis I, Hatziefhimiou A et al. TNFalpha induces expression of HIF-1alpha mRNA and protein but inhibits hypoxic stimulation of HIF-1 transcriptional activity in airway smooth muscle cells. J Cell Physiol 2013 Jan 28 [PMID: 23359428] (IHC-P, Rabbit)
• Storti P, Bolzoni M, Donofrio G et al. Hypoxia-inducible factor (HIF)-1alpha suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis and bone destruction. Leukemia 2013 Jan 24 [PMID: 23344526] (IHC-P, Mouse)
• Momose K, Minami A, Shimono Y et al. miR-214 and hypoxia down-regulate Necl-2/CADM1 and enhance ErbB2/ErbB3 signaling Genes Cells 2013 Jan 10 [PMID: 23301758] (WB, Human)
• Monte E, Chen H, Kolmakova M et al. Quantitative Analysis of Chromatin Proteomes in Disease J Vis Exp 2012 Dec [PMID: 23299252]
• Chiang CK, Nangaku M, Tanaka T et al. Endoplasmic reticulum (ER) stress signal impairs erythropoietin production: a role for ATF4 Am J Physiol Cell Physiol 2012 Dec 12 [PMID: 23242184] (WB, Human)
• Peier M, Walpen T, Christofori G et al. Sprouty2 expression controls endothelial monolayer integrity and quiescence Angiogenesis 2012 Dec 12 [PMID: 23232625] (WB, Mouse)
• Gammella E, Leuenberger C, Gassmann M et al. Evidence of synergistic/additive effects of sildenafil and erythropoietin in enhancing survival and migration of hypoxic endothelial cells. Am J Physiol Lung Cell Mol Physiol 2013 Feb [PMID: 23204066] (WB, Rat)
• Jeong HJ, Oh HA, Nam SY et al. The critical role of mast cell-derived hypoxia-inducible factor-1alpha in human and mice melanoma growth Int J Cancer 2012 Nov 19 [PMID: 23161568] (IHC, WB, Human, Mouse)
• Gualtieri F, Marinelli C, Longo D et al. Hypoxia Markers are Expressed in Interneurons Exposed to Recurrent Seizures Neuromolecular Med 2012 Oct 17 [PMID: 23073716] (IHC, ICC/IF, Human)
• Autheman D, Sheldon RA, Chaudhuri N et al. Glutathione peroxidase overexpression causes aberrant ERK activation in neonatal mouse cortex after hypoxic preconditioning. Pediatr Res 2012 Dec [PMID: 23007029] (Mouse)
• Todkar A, Di Chiara M, Loffing-Cueni D et al. Aldosterone deficiency adversely affects pregnancy outcome in mice Pflugers Arch 2012 Sep 2 [PMID: 22941338] (WB, Mouse)
• Aro E, Khatri R, Gerard-O'Riley R et al. Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes J Biol Chem 2012 Aug 28 [PMID: 22930750] (WB, Mouse)
• Lin M, Hu Y, Chen Y et al. Impacts of hypoxia-inducible factor-1 knockout in the retinal pigment epithelium on choroidal neovascularization Invest Ophthalmol Vis Sci. 2012 Sep 14 [PMID: 22915031] (IHC, Mouse)
• Tanaka T, Yamaguchi J, Shoji K, Nangaku M. Anthracycline inhibits recruitment of hypoxia-inducible transcription factors and suppresses tumor cell migration and cardiac angiogenic response in the host J Biol Chem 2012 Aug 20 [PMID: 22908232] (WB, ChIP, Human)
• Muratsu-Ikeda S, Nangaku M, Ikeda Y et al. Downregulation of miR-205 Modulates Cell Susceptibility to Oxidative and Endoplasmic Reticulum Stresses in Renal Tubular Cells plos One 2012 [PMID: 22859986] (WB, Human)
• Hatano N, Itoh Y, Suzuki H et al. Hypoxia-inducible Factor-1alpha (HIF1alpha) Switches on Transient Receptor Potential Ankyrin Repeat 1 (TRPA1) Gene Expression via a Hypoxia Response Element-like Motif to Modulate Cytokine Release J Biol Chem 2012 Sep 14 [PMID: 22843691] (WB, ChIP, Human)
• Basic VT, Tadele E, Elmabsout AA et al. Exposure to cigarette smoke induces overexpression of von Hippel-Lindau tumor suppressor in mouse skeletal muscle Am J Physiol Lung Cell Mol Physiol 2012 Sep [PMID: 22842216] (WB, Mouse)
• Shimojo Y, Akimoto M, Hisanaga T et al. Attenuation of reactive oxygen species by antioxidants suppresses hypoxia-induced epithelial-mesenchymal transition and metastasis of pancreatic cancer cells Clin Exp Metastasis 2012 Jul 26 [PMID: 22833345] (ICC/IF, Human)
• Omarova S, Charvet CD, Reem RE et al. Abnormal vascularization in mouse retina with dysregulated retinal cholesterol homeostasis J Clin Invest 2012 Aug [PMID: 22820291] (IHC-Fr, ICC/IF, Mouse)
• Qi Y, Tian X, Liu J et al. Bnip3 and AIF cooperate to induce apoptosis and cavitation during epithelial morphogenesis J Cell Biol 2012 Jul 2 [PMID: 22753893] (WB, Mouse)
• Branitzki-Heinemann K, Okumura CY, Vollger L et al. A novel role for the transcription factor HIF-1alpha in the formation of mast cell extracellular traps Biochem J 2012 Aug 15 [PMID: 22738198] (ICC/IF, Human)
• Zhu Y, Zhao T, Itasaka S et al. Involvement of decreased hypoxia-inducible factor 1 activity and resultant G1-S cell cycle transition in radioresistance of perinecrotic tumor cells Oncogene 2012 Jun 18 [PMID: 22710721] (IHC, Human)
• Shain K. Metastatic myeloma? Blood 2012 Jun 14 [PMID: 22700691] (FLOW, Human)
• Zheng X, Zheng X, Wang X et al. Acute hypoxia induces apoptosis of pancreatic ?-cell by activation of the unfolded protein response and upregulation of CHOP Cell Death Dis 2012 Jun 14 [PMID: 22695615] (WB, Mouse)
• Bardella C, Olivero M, Lorenzato A et al. Cells Lacking the Fumarase Tumor Suppressor Are Protected from Apoptosis through a Hypoxia-Inducible Factor-Independent, AMPK-Dependent Mechanism Mol Cell Biol 2012 Aug [PMID: 22645311] (WB, Human)
• Lange CA, Luhmann UF, Mowat FM et al. Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development Development 2012 Jul [PMID: 22627278] (IHC, ICC/IF, Mouse)
• Becquart P, Cambon-Binder A, Monfoulet LE et al. Ischemia Is the Prime but Not the Only Cause of Human Multipotent Stromal Cell Death in Tissue-Engineered Constructs In Vivo Tissue Eng Part A 2012 Jun 25 [PMID: 22578283] (IHC, Human)
• Fine JM, Baillargeon AM, Renner DB, Hoerster NS, Tokarev J, Colton S, Pelleg A, Andrews A, Sparley KA, Krogh KM, Frey WH, Hanson LR. Intranasal deferoxamine improves performance in radial arm water maze, stabilizes HIF-1?, and phosphorylates GSK3? in P301L tau transgenic mice. Exp Brain Res;219(3):381-90. 2012 Jun. [PMID: 22547371] (WB, Mouse)
• Bandau S, Knebel A, Gage ZO, Wood NT, Alexandru G. UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1? accumulation. BMC Biol;10:36. 2012 Apr 26. [PMID: 22537386] (WB, Human)
• Yeping Chen, Lu Fu, Ying Han et al. Testosterone replacement therapy promotes angiogenesis after acute myocardial infarction by enhancing expression of cytokines HIF-1a, SDF-1a and VEGF European Journal of Pharmacology 2012 Mar 30 [PMID: 22484505] (IHC, Rat)
• Xu Y, Li Y, Pang Y et al. Blockade of p53 by HIF-2a, but not HIF-1a, is involved in arsenite-induced malignant transformation of human bronchial epithelial cells Arch Toxicol 2012 Mar 24 [PMID: 22447124] (WB, Human)
• Chen L, Qiu JH, Zhang LL, Luo XD. Adrenomedullin promotes human endothelial cell proliferation via HIF-1 alpha Mol Cell Biochem 2012 Mar 11 [PMID: 22406979] (WB, IHC, Human)
• Garcia-Cao I, Song MS, Hobbs RM et al. Systemic Elevation of PTEN Induces a Tumor-Suppressive Metabolic State Cell 2012 Mar 30 [PMID: 22401813] (WB, Mouse)
• Azab AK, Hu J, Quang P et al. Hypoxia promotes dissemination of multiple myeloma through acquisition of epithelial to mesenchymal transition-like features Blood 2012 Jun 14 [PMID: 22394600]
• Valle-Casuso JC, Gonzalez-Sanchez A, Medina JM, Tabernero A. HIF-1 and c-Src Mediate Increased Glucose Uptake Induced by Endothelin-1 and Connexin43 in Astrocytes. PLoS One;7(2):e32448. 2012 [PMID: 22384254]
• Okumura CY, Hollands A, Tran DN et al. A new pharmacological agent (AKB-4924) stabilizes hypoxia inducible factor-1 (HIF-1) and increases skin innate defenses against bacterial infection J Mol Med (Berl) 2012 Feb 28 [PMID: 22371073] (WB, Mouse)
• Zhang S, Han C-H, Chen X-S et al. Transient Ureteral Obstruction Prevents against Kidney Ischemia/Reperfusion Injury via Hypoxia-Inducible Factor (HIF)-2[alpha] Activation. PLoS ONE 20127(1):e29876. [PMID: 22295069]
• Schwab LP, Peacock DL, Majumdar D et al. Hypoxia-inducible factor 1 alpha promotes primary tumor growth and tumor-initiating cell activity in breast cancer. Breast Cancer Res. Jan. 2012 [PMID: 22225988] (WB, Mouse)
• Xekouki P, Pacak K, Almeida M et al. Succinate dehydrogenase (SDH) D subunit (SDHD) inactivation in a growth-hormone-producing pituitary tumor: a new association for SDH? J Clin Endocrinol Metab 2012 Mar [PMID: 22170724] (Human)
• Flugel D, Gorlach A, Kietzmann T. Glycogen synthase kinase-3[beta] regulates cell growth, migration and angiogenesis via Fbw7 and USP-28-dependent degradation of hypoxia-inducible factor-1[alpha]. Blood. 2011 Dec 5. [PMID: 22144179]
• Tan SC, Carr CA, Yeoh KK, Schofield CJ, Davies KE, Clarke K. Identification of valid housekeeping genes for quantitative RT-PCR analysis of cardiosphere-derived cells preconditioned under hypoxia or with prolyl-4-hydroxylase inhibitors. Mol Biol Rep;39(4):4857-67. 2012 Apr. [PMID: 22065248] (WB, Rat)
• Oglea, M, Xiaohuan G, Espineraa A, Wei, Long. Inhibition of prolyl hydroxylases by dimethyloxaloylglycine after stroke reduces ischemic brain injury and requires hypoxia inducible factor-1a. Neurobiol Dis45(2):733-42. 2012 Feb. [PMID: 22061780]
• Shen C, Beroukhim R, Schumacher S et al. Genetic and Functional Studies Implicate HIF1alpha as a 14q Kidney Cancer Suppressor Gene. Cancer Discovery. 2011 Jun 7. [PMID: 22037472]
• Jolly S, Journiac N, Naudet F et al. Cell-Autonomous and Non-Cell-Autonomous Neuroprotective Functions of ROR{alpha} in Neurons and Astrocytes during Hypoxia. J Neurosci;31(40):14314-23. 2011 Oct 5. [PMID: 21976517]
• Olson N, Hristova M, Heintz NH et al. Activation of Hypoxia-Inducible Factor-1 Protects Airway Epithelium Against Oxidant-Induced Barrier Dysfunction. Am J Physiol Lung Cell Mol Physiol. 2011 Sep 16. [PMID: 21926263]
• Miyagi E, Andrew A, Kao S et al. Antibody-mediated enhancement of HIV-1 and HIV-2 production from BST-2/tetherin-positive cells. J Virol 2011 Nov [PMID: 21917971] (FLOW)
• Tandon R, SenGupta D, Ndhlovu LC et al. Identification of human endogenous retrovirus-specific T cell responses in vertically HIV-1-infected subjects. J Virol 2011 Nov [PMID: 21880743] (WB)
• Vengellur A, Grier E, LaPres JJ. The Loss of HIF1[alpha] Leads to Increased Susceptibility to Cadmium-Chloride-Induced Toxicity in Mouse Embryonic Fibroblasts. Journal of Toxicology. 201. [PMID: 21811500]
• Shafighi M, Olariu R, Fathi AR, Djafarzadeh S, Jakob SM, Banic A, Constantinescu MA. Dimethyloxalylglycine stabilizes HIF-1? in cultured human endothelial cells and increases random-pattern skin flap survival in vivo. Plast Reconstr Surg;128(2):415-22. 2011 Aug. [PMID: 21788833] (WB, Human)
• Lao T, Chen S, Sang N. Two mutations impair the stability and function of ubiquitin-activating enzyme (E1). J Cell Physiol doi: 10 1002/jcp. 2011 Jun 15. [PMID: 21678405]
• Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1 Mol Cell 2011 Jun 10 [PMID: 21658608] (WB, Human)
• Zhang, C, Lu, L, Li, Yet al. IGF binding protein-6 expression in vascular endothelial cells is induced by hypoxia and plays a negative role in tumor angiogenesis Cancer 2012 [PMID: 21618524] (WB, Human)
• Sparkenbaugh EM, Saini Y, Greenwood KK et al. The Role of Hypoxia Inducible Factor-1 alpha (HIF-1{alpha}) in Acetaminophen Hepatotoxicity. J Pharmacol Exp Ther. 2011 May 16. [PMID: 21576378]
• Lin Q, Cong X, Yun Z. Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha Mol Cancer Res 2011 Jun [PMID: 21571835] (WB, Human)
• Zhang L, Huang H, Cheng J, Liu J, Zhao H, Vizcaychipi MP, Ma D. Pre-treatment with isoflurane ameliorates renal ischemic-reperfusion injury in mice. Life Sci;88(25-26):1102-7. 2011 Jun 20. [PMID: 21565202] (WB, Mouse)
• Polosukhin VV, Cates JM, Lawson WE et al. Hypoxia-inducible factor-1 signalling promotes goblet cell hyperplasia in airway epithelium. J. Pathol. [PMID: 21557221]
• Lange C, Heynen SR, Tanimoto N et al. Normoxic activation of hypoxia inducible factors in photoreceptors provides transient protection against light induced retinal degeneration. Invest Ophthalmol Vis Sci. 2011 Mar 29. [PMID: 21447692]
• Francis KR, Wei L. Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning. Cell Death Dis 2010 [PMID: 21364630] (WB)
• Lin M, Chen Y, Jin J et al. Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Muller cells. Diabetologia. 2011 Jun. [PMID: 21360191] (WB, IHC, Mouse)
• Anglesio MS, George J, Kulbe H et al. IL6-STAT3-HIF signaling and therapeutic response to the angiogenesis inhibitor sunitinib in ovarian clear cell cancer. Clin Cancer Res 2011 Apr 15 [PMID: 21343371] (IHC, Human)
• Sato Y, Endo H, Okuyama H et al. Cellular hypoxia of pancreatic beta-cells due to high levels of oxygen consumption for insulin secretion in vitro. J Biol Chem 2011 Apr 8 [PMID: 21296882] (WB, Mouse, Human)
• Hoelscher M, Silter M, Krull S et al. Cardiomyocyte-specific Prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischaemic injury. J Biol Chem. 2011 [PMID: 21270129]
• Caprara C, Thiersch M, Lange C et al. HIF1A is essential for the development of the intermediate plexus of the retinal vasculature. Invest Ophthalmol Vis Sci. 2011 [PMID: 21212189]
• Schelter F, Halbgewachs B, Baumler P, Neu C, Gorlach A, Schrotzlmair F, Kruger A. Tissue inhibitor of metalloproteinases-1-induced scattered liver metastasis is mediated by hypoxia-inducible factor-1 alpha. Clin Exp Metastasis;28(2):91-9. 2011 Feb. [PMID: 21053058] (IHC-Fr, WB, Mouse)
• Antoniou X, Gassmann M, Ogunshola OO. Cdk5 interacts with Hif-1a in neurons: a new hypoxic signalling mechanism?. Brain Res 2011 Mar 24 [PMID: 20977891]
• Lam G. Exploring the Role of Hypoxia-Related Parameters in the Vascularization of Modular Tissues. Thesis 2013 (IHC-P, Human)
• Tawari EP. Investigation of chemoresistant mechanisms in triple negative breast. Thesis. Mar 1 2016 (WB, ICC/IF, Human)
• Pandya PH, Wilkes DS. HIF-1alpha regulates CD55 expression in airway epithelium. Thesis. 2015 Sep 01 (IHC-P, WB, Mouse, Human)
• Lange C, Caprara C, Tanimoto N et al. Retina-specific activation of a sustained hypoxia-like response leads to severe retinal degeneration and loss of vision. Neurobiol Dis. 2010 Sep 9. [PMID: 20817091]
• Ferguson BW, Datta S. Role of Heparan Sulfate 2-O-Sulfotransferase in Prostate Cancer Cell Proliferation, Invasion, and Growth Factor Signaling. Prostate Cancer;2011:1-14. 2011
• Gulliksson M, Carvalho RF, Ulleras E et al. Mast cell survival and mediator secretion in response to hypoxia. PLoS One 2010 2010 Aug 23 [PMID: 20808808] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB100-479, catalog number NB100-479H.
• Gupta PK, DiPette DJ, Supowit SC. Protective effect of resveratrol against pressure overload?induced heart failure. Food Science & Nutrition 3/5/2014 (WB, Mouse)
• Moslehi J, Minamishima YA, Shi J et al. Loss of hypoxia-inducible factor prolyl hydroxylase activity in cardiomyocytes phenocopies ischemic cardiomyopathy. Circulation 2010 Sep 7 [PMID: 20733101] (WB, Mouse)
• Ni A, Lashnits E, Yao LC et al. Rapid remodeling of airway vascular architecture at birth. Dev Dyn 2010 Sep [PMID: 20730909] (IHC-Fr, ICC/IF, Mouse)
• Raitila A, Lehtonen HJ, Arola J et al. Mice with inactivation of aryl hydrocarbon receptor-interacting protein (Aip) display complete penetrance of pituitary adenomas with aberrant ARNT expression. Am J Pathol 2010 Oct [PMID: 20709796] (IHC-P, Mouse)
• Olson N, Kasahara DI, Hristova M e al. Modulation of NF-kB and hypoxia-inducible factor--1 by S-nitrosoglutathione does not alter allergic airway inflammation in mice. Am J Respir Cell Mol Biol 2011 Jun [PMID: 20693401] (WB, Mouse)
• J Hyvarinen, I E Hassinen, R Sormunen et al. Hearts of Hypoxia-inducible Factor Prolyl 4-Hydroxylase-2 Hypomorphic Mice Show Protection against Acute Ischemia-Reperfusion Injury. J Biol Chem 285 (18): 13646-13657. 2010 [PMID: 20185832]
• Lee SB, Wong AP, Kanasaki K et al. Preeclampsia: 2-methoxyestradiol induces cytotrophoblast invasion and vascular development specifically under hypoxic conditions. Am J Pathol 2010 Feb [PMID: 20075204] (WB, IHC-P, Human)
• Luo T, Zhang X, Nicholson WL et al. Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosis. Clin Vaccine Immunol 2010 Jan [PMID: 19955322] (WB)
• Kim Y, Lin Q, Zelterman D et al. Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity. Cancer Res;69(24):9271-80. 2009 Dec 15. [PMID: 19934310]
• Kearon J. Selective damage to and protection of enteric neurons in vitro in models of ischemia and reperfusion. Thesis. 2014 (IHC-P, ICC/IF)
• Saini Y, Kim KY, Lewandowski R et al. The Role of Hypoxia Inducible Factor 1{alpha} (HIF1{alpha}) in Modulating Cobalt-Induced Lung Inflammation. Am J Physiol Lung Cell Mol Physiol. 2009 Nov 13. [PMID: 19915160]
• Noguera R, Fredlund E, Piqueras M et al. HIF-1alpha and HIF-2alpha are differentially regulated in vivo in neuroblastoma: high HIF-1alpha correlates negatively to advanced clinical stage and tumor vascularization. Clin Cancer Res;15(23):7130-6. 2009 Dec 1. [PMID: 19903792] (IHC-P, Human)
• Brokers N, Le-Huu S, Vogel S et al. Increased chemoresistance induced by inhibition of HIF-prolyl-hydroxylase domain enzymes. Cancer Sci 2010 Jan [PMID: 19817749]
• Minamishima YA, Moslehi J, Padera RF et al. A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo. Mol Cell Biol;29(21):5729-41. 2009 Nov. [PMID: 19720742] (WB, Mouse)
• Inagaki T, Tachibana M, Magoori K et al. Obesity metabolic syndrome in histone demethylase JHDM2a-deficient mice. Genes Cells 2009;14(8):991-1001. [PMID: 19624751] (WB, Mouse)
• Hansen SL, Trakooljul N, Liu HC et al. Iron transporters are differentially regulated by dietary iron, and modifications are associated with changes in manganese metabolism in young pigs. J Nutr 139(8):1474-9. 2009 Aug. [PMID: 19535423]
• Thiersch M, Lange C, Joly S et al. Retinal neuroprotection by hypoxic preconditioning is independent of hypoxia-inducible factor-1 alpha expression in photoreceptors. Eur J Neurosci 2009 Jun [PMID: 19508692] (WB, Mouse)
• Seavey MM, Maciag PC, Al-Rawi N et al. An anti-vascular endothelial growth factor receptor 2/fetal liver kinase-1 Listeria monocytogenes anti-angiogenesis cancer vaccine for the treatment of primary and metastatic Her-2/neu+ breast tumors in a mouse model. J Immunol 182(9):5537-46. 2009 May 1. [PMID: 19380802] (ICC/IF, IHC, Mouse)
• Zhang J, Liu A, Hou R et al. Salidroside protects cardiomyocyte against hypoxia-induced death: a HIF-1alpha-activated and VEGF-mediated pathway. Eur J Pharmacol 2009 Apr 1 [PMID: 19326475] (Rat)
• Ishikawa T, Nakashiro K, Klosek SK et al. Hypoxia enhances CXCR4 expression by activating HIF-1 in oral squamous cell carcinoma. Oncol Rep 2009 Mar [PMID: 19212630] (WB, Human)
• Wirthner R, Wrann S, Balamurugan K et al. Impaired DNA double-strand break repair contributes to chemoresistance in HIF-1 alpha-deficient mouse embryonic fibroblasts. Carcinogenesis 29(12):2306-16. 2008 Dec. [PMID: 18842680] (WB, Mouse)
• Deli L, Wittmann G, Kallo I et al. Type 1 cannabinoid receptor-containing axons innervate hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons. Endocrinology 2009 Jan [PMID: 18818298] (IHC, Mouse)
• Ream MA, Chandra R, Peavey M et al. High oxygen prevents fetal lethality due to lack of catecholamines. Am J Physiol Regul Integr Comp Physiol 295(3):R942-53. 2008 Sep. [PMID: 18635452] (WB, Mouse)
• Thiersch M, Raffelsberger W, Frigg R et al. Analysis of the retinal gene expression profile after hypoxic preconditioning identifies candidate genes for neuroprotection. BMC Genomics 2008 Feb 8 [PMID: 18261226] (WB, Mouse)
• Minamishima YA, Moslehi J, Bardeesy N et al. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood 111(6):3236-44. 2008 Mar 15. [PMID: 18096761] (WB, Mouse)
• Liao D, Corle C, Seagroves TN et al. Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression. Cancer Res 67(2):563-72. 2007 Jan 15. [PMID: 17234764] (WB, Mouse)
• Ramanathan M, Pinhal-Enfield G, Hao I et al. Synergistic up-regulation of vascular endothelial growth factor (VEGF) expression in macrophages by adenosine A2A receptor agonists and endotoxin involves transcriptional regulation via the hypoxia response element in the VEGF promoter. Mol Biol Cell 18(1):14-23. 2007 Jan. [PMID: 17065555] (WB, Mouse)
• McLaren AT, Marsden PA, Mazer CD et al. Increased expression of HIF-1alpha, nNOS, and VEGF in the cerebral cortex of anemic rats. Am J Physiol Regul Integr Comp Physiol 292(1):R403-14. 2007 Jan. [PMID: 16973934] (WB, IHC, ICC/IF, Rat)
• Kariuki TM, Van Dam GJ, Deelder AM et al. Previous or ongoing schistosome infections do not compromise the efficacy of the attenuated cercaria vaccine. Infect Immun 2006 Jul [PMID: 16790771]
• Bayele HK, Peyssonnaux C, Giatromanolaki A et al. HIF-1 regulates heritable variation and allele expression phenotypes of the macrophage immune response gene SLC11A1 from a Z-DNA forming microsatellite. Blood 110(8):3039-48. 2007 Oct 15. [PMID: 1760676] (WB, Mouse)
• Kundu CN, Balusu R, Jaiswal AS et al. Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis 28(10):2089-95. 2007 Oct. [PMID: 1752206] (WB, Mouse)
• Beck-schimmer B, Maggiorini M. Arginine-vasopressin marker copeptin is a sensitive plasma surrogate of hypoxic exposure. Hypoxia. 2014 Sep 01 (WB, Rat)
• Saini Y, Harkema JR, LaPres JJ. HIF1alpha is essential for normal intrauterine differentiation of alveolar epithelium and surfactant production in the newborn lung of mice. J Biol Chem 283(48):33650-7. 2008 Nov 28. [PMID: 18801745] (WB, Mouse)

Reviews for HIF-1 alpha Antibody (NB100-479) (20)

Average Rating: 4.4

(Based on 20 reviews)

We have 20 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Anonymous IHC-P Mouse 10/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Lot: AK-4

reviewed by: Kristina Cook WB Human 02/23/2017

Summary

• Application: Western Blot
• Sample Tested: U251 glioma cell line; whole cell lysate
• Species: Human

Comments

• Comments: 4 hour hypoxia (0.5% oxygen) incubation

reviewed by: Anonymous WB Mouse 09/21/2016

Summary

• Application: Western Blot
• Sample Tested: Mouse kidney
• Species: Mouse

Blocking

• Blocking Details: Blocked with 5% Nonfat Dry Milk in 1x TBS-T, 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: Dilution - 5% BSA in TBS-T solution buffer

Secondary Antibody

• Secondary Description: Horse Anti-mouse IgG, HRP-linked Antibody
• Secondary Manufacturer Cat#: #7076
• Secondary Concentration: 2ul in 10ml of 5% Milk in TBS-T

Details

• Detection Notes: Chemiluminescent detection

reviewed by: Anonymous WB Mouse 08/06/2016

Summary

• Application: Western Blot
• Species: Mouse
• File: View PDF

reviewed by: Zhongxiao Wang WB Mouse 10/05/2015

Summary

• Application: Western Blot
• Sample Tested: mouse retina
• Species: Mouse
• Lot: NB100-479

Blocking

• Blocking Details: 5%milk/T-BST buffer, room temparature, 2hr

Primary Anitbody

• Dilution Ratio: 1:1000 in 5%milk/T-BST buffer, 4 degree, overnight

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: Sigma AQ132P
• Secondary Concentration: 1:3000

Details

• Detection Notes: Develop machine, 30s exposure shows clear bands

Comments

• Comments: There's a non-specific band above for mouse retina samples

reviewed by: Anonymous WB Human 08/23/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

Blocking

• Blocking Details: Blocking with 5% non-fat milk for 30 min

Primary Anitbody

• Dilution Ratio: 1:1000, overnight

Secondary Antibody

• Secondary Description: Donkey anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: GE Healthcare, NA934V
• Secondary Concentration: 1:2000

Details

• Detection Notes: Regular Protocol

reviewed by: Anonymous WB Human 03/27/2015

Summary

• Application: Western Blot
• Sample Tested: Hela lysates treated with MG132
• Species: Human

Blocking

• Blocking Details: 5% milk in TBST

Primary Anitbody

• Dilution Ratio: 1:2000

Secondary Antibody

• Secondary Description: Goat anti-rabbit

Details

• Detection Notes: using the regular pico from pierece, detection by Bio-rad imager, 1 min exposure time, DMSO as the NC, MG132 as the PC

reviewed by: Anonymous WB Mouse 02/18/2015

Summary

• Application: Western Blot
• Sample Tested: Mouse heart
• Species: Mouse
• Lot: J1

Blocking

• Blocking Details: 5% milk in TBST for 1h at RT

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% milk TBST overnight at 4°C

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG, HRP-coupled, 1:10 000 in 5% milk TBST, 1h at RT
• Secondary Manufacturer Cat#: AffiniPure Goat Anti-Rabbit IgG (H+L) from Jackson Immunolab
• Secondary Concentration: 1:10 000

Details

• Detection Notes: ECL Clarity, exposure time 30s

Comments

• Comments: - The signal was strong, it might be useful to decrease primary antibody concentration to reduce background noise.

reviewed by: Anonymous ChIP Human 12/15/2014

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: HEK293
• Species: Human
• Lot: AA-1

Primary Anitbody

• Dilution Ratio: Use 5ug antibody per 1ml lysate, and incubate at 4oC overnight

Details

• Detection Notes: Real-time PCR.
• Fixation/Cross-Linking: Elute the beads with 1% SDS, 0.1M NaHCO3 and then reverse the cross-linking by incubating at 65oC for 6 hours.
• DNS Sharing: Covaris sonicator, 6 seconds for 200-500bp
• Immunoprecipitation Details: Millipore protein G magnetic beads 50ul/1ml lysate at room temperature for 10 minutes.
• Crosslinking/Reversal Details: 1% formaldehyde for 5 minutes at room temperature
• DNA Prurification Details: Qiagen PCR cleanup kit.

reviewed by: Anonymous WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23232625
• Species: Mouse

Blocking

• Blocking Details: See PMID 23232625

Details

• Detection Notes: See PMID 23232625

Comments

• Comments: This antibody against HIF-1alpha reproducibly recognizes a single band in western blots assessing protein lysates from hypoxia-treated primary mouse aortic endothelial cells.Published in PMID: 23232625.

reviewed by: Xiuquan Luo WB Human 09/12/2014

Summary

• Application: Western Blot
• Sample Tested: HEK293 cell lysate
• Species: Human
• Comments: Antibody detects some non-specific bands, but give a good HIF1a signal at indicated MW.

Blocking

• Blocking Details: 1xPBST with 5% non-fat milk, RT 1 hour

Primary Anitbody

• Dilution Ratio: 1xPBST with 5% non-fat milk, overnight 4C, 1:1000 dilution

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG-HRP
• Secondary Manufacturer Cat#: sc-2004
• Secondary Concentration: 1:2000 dilution

Details

• Detection Notes: ECL dura, exposured for 2-5 min

Comments

• Comments: Antibody detects some non-specific bands, but give a good HIF1a signal at indicated MW.

reviewed by: Ming Lu WB Human 08/15/2014

Summary

• Application: Western Blot
• Sample Tested: Human Pancreatic Cancer Cell line
• Species: Human
• Lot: AA
• Comments: Very clean antibody, but it has multiple bands.

Blocking

• Blocking Details: 2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

• Dilution Ratio: 1:1000 overnight at 4C in blocking buffer

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: Cell Signaling, #7074
• Secondary Concentration: 1:5000

Details

• Detection Notes: 1 min exposure shows band at correct MW.

Comments

• Comments: Very clean antibody, but it has multiple bands.

reviewed by: Molly Jenkins WB Human 07/18/2014

Summary

• Application: Western Blot
• Sample Tested: Nuclear fraction, Human cell line
• Species: Human

Blocking

• Blocking Details: 5% milk in TBST, 1hr at RT

Primary Anitbody

• Dilution Ratio: 1:1000

Secondary Antibody

• Secondary Description: Goat anti-rabbit HRP
• Secondary Concentration: 1:20,000

reviewed by: Jason Lee WB Human 10/29/2013

Summary

• Application: Western Blot
• Sample Tested: Human MCF7 breast epithelial cells
• Species: Human
• Lot: Lot X-1
• Comments: nuclear extract resolved in 8% SDS-PAGE gel

Blocking

• Blocking Details: 3% BSA in TBST buffer 1 hour room temp

Primary Anitbody

• Dilution Ratio: 1:2000 dilution in 3% BSA TBST 1 hour room temp

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG HRP
• Secondary Manufacturer Cat#: Sigma
• Secondary Concentration: 1:5000 in 5% skim milk TBST

Details

• Detection Notes: LAS4000 2 second exposure

Comments

• Comments: nuclear extract resolved in 8% SDS-PAGE gel

reviewed by: Anonymous IHC-P Mouse 06/27/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: paraffin section of skin wound
• Species: Mouse
• Comments: Antibody works much better when you find the necessary dilution which is quite low. Works best around 1:5000 to 1:7000.

Primary Anitbody

• Dilution Ratio: 1:6000, room tempereature in Dako antibody diluent, overnight, 4oC

Secondary Antibody

• Secondary Description: anti rabbit HRP polymer, Dako

Details

• Fixation Details: 4% paraformaldehyde, DAKO heat mediated antigen retrieval, 20 mins, 98 deg C
• Wash Description: Dako wash buffer, Tris, contains Tween 20

Comments

• Comments: Antibody works much better when you find the necessary dilution which is quite low. Works best around 1:5000 to 1:7000.

reviewed by: Sandhya Khurana WB Rat 02/05/2012

Summary

• Application: Western Blot
• Sample Tested: PC12 nuclear extract, Sample Amount: 50ug
• Species: Rat

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in TBST, Blocking Time: 30 minutes, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk/TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

• Secondary Description: Secondary Ab Dilution Ratio: 1:2000

Details

• Detection Notes: Method:ECL, Time:2min, PosCtrl: Lysate from cells overexpressing HIF1a, NegCtrl: Untreated cells, Specific Bands:110-120kDa

reviewed by: Anonymous WB Rat 01/03/2012

Summary

• Application: Western Blot
• Sample Tested: PC12 cell whole lysate, Sample Amount: 25ug
• Species: Rat
• Lot: P1
• Comments: This antibody detected HIF-1a.

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4 C

Comments

• Comments: This antibody detected HIF-1a.

reviewed by: Anonymous IHC-P Mouse 07/30/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: normal mouse kidney
• Species: Mouse
• Comments: Nuclear staining of (active) Hif-1alpha + cytoplasmic staining of (inactive) Hif-1alpha in normal mouse kidney (formalin-fixed, paraffin-embedded). No staining detected in controls. Staining done according to CSAII kit instructions. In addition, a permeabilization step was included (20 min. PBS + 0.5% Triton X-100) prior to Protein Block. Detection Method: DAB, Detection Time: 10 minutes, Positive Control: normal mouse kidney, Negative Control: rabbit isotype, antibody pre-absorbed with Hif-1alpha protein (NBC1-18422), Deparaffinization: 2x5 min. xylene, Antigen Retrieval: Target Retrieval Solution (Dako), 25 min. in pressure cooker

Blocking

• Blocking Details: Blocking Buffer: Protein Block (CSAII kit, Dako), Blocking Time: 15 minutes, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:20,000, Incubation Dilution Buffer:Ab Diluent, background reducing (Dako), Incubation Time/Temp:overnight @ 4C

Secondary Antibody

• Secondary Description: Secondary Ab: CSAII Rabbit Link (Dako), Secondary Ab Dilution: 1:1
• Secondary Manufacturer Cat#: K1501

Details

• Detection Notes: Please see Comments
• Fixation Details: Preparation: perfused PBS-formalin, postfixed overnight @ 4C in formalin, paraffin-embedded, Deparaffinization: 2x5 min. xylene

Comments

• Comments: Nuclear staining of (active) Hif-1alpha + cytoplasmic staining of (inactive) Hif-1alpha in normal mouse kidney (formalin-fixed, paraffin-embedded). No staining detected in controls. Staining done according to CSAII kit instructions. In addition, a permeabilization step was included (20 min. PBS + 0.5% Triton X-100) prior to Protein Block. Detection Method: DAB, Detection Time: 10 minutes, Positive Control: normal mouse kidney, Negative Control: rabbit isotype, antibody pre-absorbed with Hif-1alpha protein (NBC1-18422), Deparaffinization: 2x5 min. xylene, Antigen Retrieval: Target Retrieval Solution (Dako), 25 min. in pressure cooker

reviewed by: Paramahamsa Maturu IHC-P Human 03/01/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Kidney tumor
• Species: Human
• Lot: I
• Comments: That works great without any background staining in the negative control. Detection Method: ABC and DAB, Positive Control: Glioblastoma, Negative Control: Without primary Ab, Deparaffinization: Xylen and ethanol, Antigen Retrieval: Tris -EDTA pH 9.0, 45 minutes at 95 degrees Celsius in water bath

Blocking

• Blocking Details: Blocking Buffer:.10% Normal Goat Serum in 1XPBS, Blocking Time: 1 hour, Blocking Temp: RT in humidified chamber

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:100, Incubation Dilution Buffer: 2.5% Normal Goat Serum in 1X PBS, Incubation Time/Temp: overnight at 4C

Secondary Antibody

• Secondary Description: Secondary Ab: Biotinylated Anti Rabbit IgG (H+L) made in Goat, Secondary Ab Dilution: 1:500
• Secondary Manufacturer Cat#: BA-1000

Details

• Detection Notes: Please see Comments
• Fixation Details: Sample Preparation: Paraffin embedded, Deparaffinization: Xylen and ethanol

Comments

• Comments: That works great without any background staining in the negative control. Detection Method: ABC and DAB, Positive Control: Glioblastoma, Negative Control: Without primary Ab, Deparaffinization: Xylen and ethanol, Antigen Retrieval: Tris -EDTA pH 9.0, 45 minutes at 95 degrees Celsius in water bath

reviewed by: JORCYK LAB WB Mouse 02/01/2011

Summary

• Application: Western Blot
• Sample Tested: 4T1 cells whole cell lysate
• Species: Mouse
• Comments: The Hif1a band shows up even if the lysate concentration is very low. There are some background bands, but is acceptable, and there is quite a bit of lot to lot variations with this product... unfortunately.

Blocking

• Blocking Details: Blocking Buffer: 5% milk or 3% BSA, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:1000-1:5000, Incubation Dilution Buffer: TBS-T or PBS-T, Incubation Time: 1 hr-overnight, Incubation Temp: 4C

Secondary Antibody

• Secondary Description: Secondary Ab Dilution Ratio: 1:10,000-1:150,000

Details

• Detection Notes: Method: ECL, Exposure Time: 10sec-10min, Pos.Ctrl: ECL reactive ladder, Bands:~120kDa(specific), ~80,55,72kDa(non-specific)

Comments

• Comments: The Hif1a band shows up even if the lysate concentration is very low. There are some background bands, but is acceptable, and there is quite a bit of lot to lot variations with this product... unfortunately.

FAQs for HIF-1 alpha Antibody (NB100-479). (Showing 1 - 9 of 9 FAQs).

1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
   • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
   • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, as desired by you, I am suggesting you four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products has been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449 ) (cited in at least 31 peer reviewed publications).
3. I would like to know, does it exist path for detection HIF 1 in the venous blood before and after revascularization of the leg?
   • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
   • The theoretical molecular weight of HIF 1-alpha is ~93kDa, however, you will likely see a band between 100-120kDa due to phosphorylation.
5. Do you sell a positive and negative control for the HIF-1 alpha antibody (NB100-479)?
   • For Western blot, the best positive control that we have is a CoCl2-treated Cos-7 cell lysate, catalog # NB800-PC26. The kit comes with a negative control as well. If you are doing ICC, then you could treat your cells with CoCl2 or expose them to hypoxic conditions.
6. What do think the blocking buffer should be?
   • We recommend 5% non-fat dry milk in TBS.
7. We need to examine tissue-specific knockout of HIF-1a in mouse lungs. We have generated smooth muscle specific knockout of HIF-1a, and would like to demonstrate the knockdown in lung tissue sections by IHC or immunofluorescence. The problem is that all the antibodies for the mouse seem to have too much non-specific staining. Can you recommend an antibody that would be good for mouse lung tissue IHC or immunofluorescence?
   • The antibody with Catalog # NB100-479 is one of our best sellers and has 5 star rating in customer reviews. Please view the product datasheet to see the publications in which researchers have cited this product, over 10 of which are involved in mouse models.
8. Is there a WB image for this antibody?
   • We do have a WB image for the antibody base project. It looks like they ordered an HRP conjugated format, and that specific format page does not have the image. We do all of our testing on the base product.
9. Will this antibody detect HIF-1 alpha protein by WB on a breast cancer cell line in normal condition (without hypoxia)?
   • If the breast cancer gene turned on the HIF1 alpha gene, there should be some expression of the protein, even without hypoxic induction. However, this expression may be very low, and depending on the length of time it took to lyse and/or fix the cells, may have degraded in the presence of oxygen. This may also be affected by the presence or lack of any HIF stabilizers in the lysate buffer. We talk about these stabilizers and other testing protocols in our white paper here: http://www.novusbio.com/whitepapers/cellular-response-hypoxia.html. I would recommend trying to detect HIF expression in cancer cell lines that you run both positive and negative controls along side the cell lines to ensure that any bands detected are in fact HIF1 alpha.

Bioinformatics

• Gene Symbol: HIF1A
• Entrez:
  • 3091(Human)
  • 15251(Mouse)
  • 29560(Rat)
• Uniprot:
  • Q61221(Mouse)