Immunogen | This HIF-1 alpha antibody was developed against a fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221]. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | HIF1A |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | WB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 27148974). |
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Theoretical MW | 93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at -20C. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
PRAVEEN Dubey |
WB | Human | 05/28/2021 |
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reviewed by:
Shaima Salman |
WB | Human | 03/15/2021 |
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reviewed by:
Verified Customer |
WB | Human and Mouse | 10/10/2020 |
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reviewed by:
Jie Lan |
ChIP | Human and Human Breast Cancer Cell Lines | 05/17/2020 |
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reviewed by:
Shubham Dubey |
WB | Human | 04/16/2019 |
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reviewed by:
L P |
WB | Human | 09/18/2018 |
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reviewed by:
Serina Ng |
IHC-P | Mouse | 10/25/2017 |
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reviewed by:
Verified Customer |
WB | Human | 02/23/2017 |
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reviewed by:
Kim Lab |
WB | Mouse | 09/21/2016 |
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reviewed by:
Liyong Zhang |
WB | Mouse | 08/06/2016 |
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reviewed by:
Verified Customer |
WB | Mouse | 10/05/2015 |
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reviewed by:
Haiquan Lu |
WB | Human | 08/23/2015 |
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reviewed by:
Qing Zhang |
WB | Human | 03/27/2015 |
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reviewed by:
Pierre Thiebaut |
WB | Mouse | 02/18/2015 |
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reviewed by:
Ning Wang |
ChIP | Human | 12/15/2014 |
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reviewed by:
cynthia morimoto |
WB | Mouse | 12/12/2014 |
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reviewed by:
Ning Wang |
WB | Other | 09/15/2014 |
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reviewed by:
Verified Customer |
WB | Human | 09/12/2014 |
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reviewed by:
Ning Wang |
WB | Other | 08/18/2014 |
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reviewed by:
Verified Customer |
WB | Human | 08/15/2014 |
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reviewed by:
Verified Customer |
WB | Human | 07/18/2014 |
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reviewed by:
Verified Customer |
WB | Human | 10/29/2013 |
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reviewed by:
Ian Darby |
IHC-P | Mouse | 06/27/2013 |
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reviewed by:
Verified Customer |
WB | Rat | 02/05/2012 |
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reviewed by:
Naoharu Takano |
WB | Rat | 01/03/2012 |
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reviewed by:
Yves Heremans |
IHC-P | Mouse | 07/30/2011 |
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reviewed by:
Verified Customer |
IHC-P | Human | 03/01/2011 |
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reviewed by:
Verified Customer |
WB | Mouse | 02/01/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for HIF-1 alpha Antibody (NB100-479)Discover more about diseases related to HIF-1 alpha Antibody (NB100-479).
| Pathways for HIF-1 alpha Antibody (NB100-479)View related products by pathway.
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Hypoxia-Dependent CAR Stabilizing Construct in T cells Improves Solid Tumor Targeting and Efficacy By Victoria Osinski, PhDDespite advances in the development of cancer immunotherapies, those specifically targeting tumors still remains limited. Currently, there is great interest in utilizing chimeric antigen rece... Read full blog post. |
Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post. |
Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post. |
Read full blog post. |
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post. |
mTOR Signaling and the Tumor Microenvironment By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ... Read full blog post. |
Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ... Read full blog post. |
Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy... Read full blog post. |
Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve... Read full blog post. |
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PRAVEEN Dubey 05/28/2021 |
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Application: | WB | |
Species: | Human |
Shaima Salman 03/15/2021 |
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Application: | WB | |
Species: | Human |
Verified Customer 10/10/2020 |
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Application: | WB | |
Species: | Human and Mouse |