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287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.2 units/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA). The ND50 is typically <3 µg/mL." class="big_lightbox" target="_blank">
VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
287-TC) stimulates cell proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner. The ED50 for this effect is 0.015-0.075 units/mL." class="big_lightbox" target="_blank">
963-ER) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human Erythropoietin/EPO (287-TC) was measured at a concentration range between 0.488 pM and 1000 pM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.219 pM. " class="big_lightbox" target="_blank">
Erythropoietin (Epo) stimulates red blood cell production by docking with its cognate receptor on the erythroid progenitor cell and triggering an array of signaling pathways that inhibit apoptosis and promote cell proliferation and differentiation. In its pharmaceutical forms, epoetin and darbepoetin, Epo is widely used to treat various anemias, including those associated with cancer.