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CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.
![Immunohistochemistry Angiopoietin-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Angiopoietin-1_AF923_Immunohistochemistry_6916.jpg)
![Immunohistochemistry Angiopoietin-1 Antibody (171718) [Unconjugated]](http://images.novusbio.com/images/antibody/Angiopoietin1_MAB923_Immunohistochemistry_17350.jpg)
![ELISA Angiopoietin-1 Antibody (171733) [Unconjugated]](http://images.novusbio.com/images/mab9231_human-angiopoietin-1-mab-clone-171733-elisa-639201781558651939.jpg)
![N/A Angiopoietin-1 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Angiopoietin1_DANG10_ELISA_572.jpg)
![N/A Angiopoietin-1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Angiopoietin1_DY923_ELISA_2265.jpg)
![Immunohistochemistry Angiopoietin-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Angiopoietin-1_AF923_Immunohistochemistry_9976.jpg)
![Immunohistochemistry Angiopoietin-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Angiopoietin1_AF923_Immunohistochemistry_15338.jpg)
![Immunohistochemistry Angiopoietin-1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af923_human-mouse-angiopoietin-1-affinity-purified-polyclonal-ab-822024100611.jpg)
![Immunohistochemistry Angiopoietin-1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af923_human-mouse-angiopoietin-1-affinity-purified-polyclonal-ab-8120255485712.jpg)
![ELISA Angiopoietin-1 Antibody [Unconjugated]](http://images.novusbio.com/images/af923_human-mouse-angiopoietin-1-affinity-purified-polyclonal-ab-elisa-639201850081262180.jpg)
![Western Blot Angiopoietin-1 Antibody (171718) [Unconjugated]](http://images.novusbio.com/fullsize/mab923_human-angiopoietin-1-mab-clone-171718-4120241252127.jpg)
![Immunohistochemistry Angiopoietin-1 Antibody (171718) [Unconjugated]](http://images.novusbio.com/fullsize/mab923_human-angiopoietin-1-mab-clone-171718-41202412512620.jpg)
![N/A Angiopoietin-1 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Angiopoietin1_DANG10_ELISA_573.jpg)
![N/A Angiopoietin-1 [HRP]](http://images.novusbio.com/fullsize/elisa/Angiopoietin-1_DANG10_ELISA_29.jpg)
![Bioactivity Angiopoietin-1 [Unconjugated]](http://images.novusbio.com/images/protein/Angiopoietin1_9936AN_2068.jpg)
![SDS-Page Angiopoietin-1 [Unconjugated]](http://images.novusbio.com/fullsize/protein/Angiopoietin1_9936AN_1997.jpg)
