Glut1 Antibody

Images

 
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - (PMID: 21620138 ).
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the ...read more
Biological Strategies: Flow Cytometry: Glut1 Antibody [NB110-39113] - Analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin ...read more
Immunocytochemistry/ Immunofluorescence: Glut1 Antibody [NB110-39113] - GLUT1 antibody was tested in HEK-293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Western Blot: Glut1 Antibody [NB110-39113] - Glut 1 at 55kDa. Antibody diluted 1:500. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the ...read more
Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG cells with Glut1 Antibody NB110-39113F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, Bv, PmSpecies Glossary
Applications WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Glut1 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot# P11166]
Localization
Cell membrane, Cytoplasm (near membranes), Melanosome
Marker
Plasma Membrane Marker
Predicted Species
Primate (100%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Flow (Intracellular)
Application Notes
This GLUT1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature.In WB a band is seen at ~55 kDa on kidney membrane preps representing GLUT1 protein. Depending on the tissue and any post-translational modifications, this protein can run anywhere between 40-60 kDa. The FLOW use of this antibody was submitted by a Verified Customer (See Review on NB110-39113PE for FLOW Protocol Details.)
Control
Glut1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 7 Reviews rated 4.9
using
NB110-39113 in the following applications:

Publications
Read Publications using
NB110-39113 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 29456650). 100% sequence identity with primate, 93% sequence identity with bovine.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut1 Antibody

  • DYT17
  • DYT18
  • DYT18DYT17
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • Glut1
  • GLUT-1
  • GLUT1DS
  • GLUT1GLUT
  • HepG2 glucose transporter
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • solute carrier family 2, facilitated glucose transporter member 1

Background

Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as Glut 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. The majority of differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testis barriers. GLUT1 is a major glucose transporter at the mammalian blood brain barrier. It is ubiquitous, and is present at high levels in primate erythrocytes and brain endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut1 Antibody (NB110-39113)(25)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 7 applications: ChIP, FLOW, Flow, ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ChIP
(2)
FLOW
(2)
Flow
(2)
ICC/IF
(2)
IHC-Fr
(3)
IHC-P
(1)
WB
(12)
All Applications
Filter By Species
Human
(9)
Mouse
(11)
Rat
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB110-39113 Applications Species
Li X, Wen MZ, Su LX et al. Local suture ligation-assisted percutaneous sclerotherapy for Kasabach-Merritt phenomenon-associated kaposiform haemangioendothelioma. Oncol Lett Jan 1 2019 [PMID: 30655857] (IHC-P, Human) IHC-P Human
Appel S, Grothe J, Storck S et al. A potential role for GSK3beta in glucose-driven intrauterine catch-up growth in maternal obesity Endocrinology Dec 10 2018 [PMID: 30535296] (Mouse) Mouse
Patel S, Fu S, Mastio J et al. Unique pattern of neutrophil migration and function during tumor progression. Nat. Immunol. Nov 1 2018 [PMID: 30323345] (Flow, Mouse) Flow Mouse
Pacella I, Procaccini C, Focaccetti C et al. Fatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth Proc Proc Natl Acad Sci U S A.  2018 Jul 10 [PMID: 29941600] (Flow, Mouse)

Details:
Citation using the PE form of this antibody.
Flow Mouse
Trompette A, Gollwitzer ES, Pattaroni C et al. Dietary Fiber Confers Protection against Flu by Shaping Ly6c- Patrolling Monocyte Hematopoiesis and CD8+ T Cell Metabolism Immunity May 15 2018 [PMID: 29768180] (Mouse) Mouse
Ashok A, Singh N. Prion protein modulates glucose homeostasis by altering intracellular iron Sci Rep Apr 26 2018 [PMID: 29700330] (WB) WB
Xu X, Cui N, Wang E. Application of an acellular dermal matrix to a rabbit model of oral mucosal defects Exp Ther Med 2018 Jan 05 [PMID: 29456650] (WB, Rabbit) WB Rabbit
Ho JJD, Balukoff NC, Cervantes G, Malcolm PD. Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B. Cell Rep. 2018 Jan 02 [PMID: 29298419] (WB) WB
Thwe P, Pelgrom L, Cooper R et al. Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses. Cell Metab 2017 Sep 05 [PMID: 28877459] (FLOW, Mouse). 2017 Sep 05 [PMID: 28877459] (FLOW, Mouse)

Details:
This citation used the Alexa Fluor 488 form of this antbody
FLOW Mouse
Cai TT, Ye SB, Liu YN et al. LMP1-mediated glycolysis induces myeloid-derived suppressor cell expansion in nasopharyngeal carcinoma PLoS Pathog. 2017 Jul 01 [PMID: 28732079] (ICC/IF, Human) ICC/IF Human
Show All 25 Publications.

Reviews for Glut1 Antibody (NB110-39113) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Mouse and Human.

Reviews using NB110-39113:
Filter by Applications
WB
(5)
ICC
(2)
All Applications
Filter by Species
Human
(4)
Mouse
(2)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Glut1 NB110-39113
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reviewed by:
Anonymous
WB Mouse 04/23/2019
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotF-7
Immunocytochemistry Glut1 NB110-39113
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reviewed by:
Anonymous
ICC Human 10/24/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedbeta cells
SpeciesHuman
LotF-2

Comments

Commentsuse at 1:100
Western Blot Glut1 NB110-39113
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reviewed by:
Ajay Ashok
WB Human 06/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain
SpeciesHuman

Comments

Commentsuse at 1:500
Immunocytochemistry Glut1 NB110-39113
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reviewed by:
Ajay Ashok
ICC Mouse 06/12/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse eye
SpeciesMouse

Comments

Commentsuse at 1:100 concentration
Western Blot Glut1 NB110-39113
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reviewed by:
Ajay Ashok
WB Mouse and Human 06/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedRetina (inner nuclear layer)
SpeciesMouse and Human

Comments

CommentsUse at a conc of 1:500 for 20ug sample protein loaded
Western Blot Glut1 NB110-39113
Enlarge
reviewed by:
Marie-estelle LOSFELD
WB Human 03/09/2014
View

Summary

ApplicationWestern Blot
Sample Testedmembrane fraction from lung cancer cells
SpeciesHuman
Western Blot Glut1 NB110-39113
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reviewed by:
Anonymous
WB Human 09/01/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 20 ug
SpeciesHuman
CommentsWorks for human GLUT1

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:2000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit IgG-HRP, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 42 kDa

Comments

CommentsWorks for human GLUT1

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for Glut1 Antibody (NB110-39113). (Showing 1 - 2 of 2 FAQs).

  1. Can you tell me what if any antigen retrieval method was used for the staining of paraffin sections with GLUT1 Antibody (NB110-39113)?
    • Our lab used HIER with 0.01 M sodium citrate buffer, pH 6.0 at 99-100C for 20 minutes in a steamer. You should also be able to use a pressure cooker without any problems.
  2. Does NB110-39113 recognize the extracellular sequences or the cytoplasmic sequence?
    • Unfortunately I cannot give you the exact epitope sequence, as this information is propitiatory. However I can let you know that the epitope is located around first cytoplasmic and helical domain, but before the first extracellular domain.http://www.uniprot.org/uniprot/P11166#sequences Below is some information from UniProt regarding the topology of the Glut1 protein:Topological domain:    1 – 11    CytoplasmicTransmembrane:    12 – 33    HelicalTopological domain:    34 – 66    ExtracellularTransmembrane:    67 – 87    HelicalTopological domain:   88 – 90    CytoplasmicTransmembrane:    91 – 112    Helical

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-39113AF405
Alexa Fluor 488 Labeled NB110-39113AF488
Alexa Fluor 647 Labeled NB110-39113AF647
Alexa Fluor 700 Labeled NB110-39113AF700
Biotin Labeled NB110-39113B
DyLight 405 Labeled NB110-39113V
DyLight 488 Labeled NB110-39113G
DyLight 550 Labeled NB110-39113R
DyLight 650 Labeled NB110-39113C
FITC Labeled NB110-39113F
HRP Labeled NB110-39113H

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Bioinformatics Tool for Glut1 Antibody (NB110-39113)

Discover related pathways, diseases and genes to Glut1 Antibody (NB110-39113). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Glut1 Antibody (NB110-39113)

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Blogs on Glut1.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

Anonymous
04/23/2019
Application: WB
Species: Mouse

Anonymous
10/24/2017
Application: ICC
Species: Human

Ajay Ashok
06/12/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC2A1