Glut1 Antibody


Western Blot: Glut1 Antibody [NB110-39113] - GLUT 1 in A549 cells. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Glut1 Antibody [NB110-39113] - GLUT1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 more
Flow Cytometry: Glut1 Antibody [NB110-39113] - Analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture more
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).

Product Details

Reactivity Hu, Mu, Bovine, Primate, RabbitSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr
1 mg/ml

Order Details

Glut1 Antibody Summary

A synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot# P11166]
Cell membrane
Plasma Membrane Marker
Predicted Species
Primate (100%), Bovine (93%), Rabbit (93%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 0.5-2.0 ug/ml
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
Application Notes
This GLUT1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature.In WB a band is seen at ~55 kDa on kidney membrane preps representing GLUT1 protein. Depending on the tissue and any post-translational modifications, this protein can run anywhere between 40-60 kDa. The FLOW use of this antibody was submitted by a Verified Customer (See Review on NB110-39113PE for FLOW Protocol Details.
Reviewed Applications
Read 2 Reviews rated 5
NB110-39113 in the following applications:

Read Publications using
NB110-39113 in the following applications:

Reactivity Notes

Reacts with human and mouse. 100% sequence identity with primate protein, 93% sequence identity with cow, and rabbit proteins. Rat reactivity reported in scientific literature (PMID: 26590355)

Alternate Names for Glut1 Antibody

  • DYT17
  • DYT18
  • DYT18DYT17
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • GLUT-1
  • HepG2 glucose transporter
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • solute carrier family 2, facilitated glucose transporter member 1


GLUT1 is a major glucose transporter in the mammalian blood brain barrier. It is ubiquitous, and is present at high levels in primate erythrocytes and brain endothelial cells. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are seven types of glucose transport carrier proteins, designated as Glut 1 to 7.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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Applications: IHC, IHC-P
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Applications: ICC/IF, IHC, IHC-P
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Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr

Publications for Glut1 Antibody (NB110-39113)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ChIP, FLOW, ICC/IF, IHC-Fr, WB.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB110-39113 Applications Species
Nusken E, Gellhaus A, Kuhnel E et al. Increased Rat Placental Fatty Acid, But Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming. J. Cell. Biochem. 2015 Nov 21 [PMID:26590355] (WB, Rat) WB Rat
De Riccardis L, Rizzello A, Ferramosca A et al. Bioenergetics profile of CD4(+) T cells in relapsing remitting multiple sclerosis subjects J. Biotechnol. 2015 Feb 19 [PMID:25701681] (WB, Human) WB Human
Tambe Y, Hasebe M, Kim CJ et al. The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B Mol. Carcinog. 2015 Jan 24 [PMID:25620379] (WB, Mouse) WB Mouse
Yang F, Lu W, Lin W et al. Enhancement of Neurotrophic Factors in Astrocyte for Neuroprotective Effects in Brain Disorders Using Low-intensity Pulsed Ultrasound Stimulation. Brain Stimul. 2014 Dec 04 [PMID:25558041] (WB, Human) WB Human
Rodriguez-Espinosa O, Rojas-Espinosa O, Moreno-Altamirano MM et al. Metabolic requirements for neutrophil extracellular traps nets) formation. Immunology 2014 Dec 27 [PMID:25545227] (FLOW, Human) FLOW Human
Takeshita Y, Obermeier B, Cotleur A et al. An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture. J. Neurosci. Methods. 2014 May 22 [PMID:24858797] (ICC/IF, Human) ICC/IF Human
Moreau JL, Artap ST, Shi H et al. Cited2 is required in trophoblasts for correct placental capillary patterning. Dev. Biol. 2014 May 05 [PMID:24803182] (IHC-Fr, Mouse) IHC-Fr Mouse
Luo W, Chang R, Zhong J, Pandey A, Semenza GL. Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression. Proc Natl Acad Sci U S A. 2012 Dec 4 [PMID:23129632] (ChIP) ChIP
Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC et al. Regulation of TFEB and V-ATPases by mTORC1. EMBO J. Jul. 2011 [PMID:21804531] (WB, Mouse) WB Mouse
Luo W, Hu H, Chang R et al. Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell;145(5):732-44. 2011 May 27. [PMID:21620138] (WB, ChIP, Human) WB, ChIP Human
Show All 13 Publications.

Reviews for Glut1 Antibody (NB110-39113) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: WB.

Reviews using NB110-39113:
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Images Ratings Applications Species Date Details
Western Blot Glut1 NB110-39113
reviewed by:
WB Human 03/09/2014


ApplicationWestern Blot
Sample Testedmembrane fraction from lung cancer cells
Western Blot Glut1 NB110-39113
reviewed by:
WB Human 09/01/2010


ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 20 ug
CommentsWorks for human GLUT1


Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:2000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit IgG-HRP, Secondary Ab Dilution Ratio: 1:5000


Detection NotesDetection Method: ECL Plus, Specific Bands: 42 kDa


CommentsWorks for human GLUT1

Product General Protocols

View specific protocols for Glut1 Antibody (NB110-39113): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Glut1 Antibody (NB110-39113). (Showing 1 - 1 of 1 FAQs).

  1. Can you tell me what if any antigen retrieval method was used for the staining of paraffin sections with GLUT1 Antibody (NB110-39113)?
    • Our lab used HIER with 0.01 M sodium citrate buffer, pH 6.0 at 99-100C for 20 minutes in a steamer. You should also be able to use a pressure cooker without any problems.

Secondary Antibodies


Isotype Controls

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Blogs on Glut1.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SLC2A1

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