Glut1 Antibody

Images

 
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza (PMID: 21620138).
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB ...read more
Immunocytochemistry/ Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Biological Strategies: Flow Cytometry: Glut1 Antibody [NB110-39113] - Flow cytometry analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 ...read more
Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).
Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.
Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, Bv, PmSpecies Glossary
Applications WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Glut1 Antibody Summary

Immunogen
This Glut1 antibody is made against a synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot# P11166].
Localization
Cell membrane, cytoplasm (near membranes), melanosome
Marker
Plasma Membrane Marker
Predicted Species
Primate (100%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • In vitro assay
  • Flow (Intracellular)
Application Notes
This GLUT1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature. In WB a band is seen at ~55 kDa on kidney membrane preps representing GLUT1 protein. Depending on the tissue and any post-translational modifications, this protein can run anywhere between 40-60 kDa. This Glut1 antibody is validated for Flow cytometry from a verified customer review. Use In vitro assay reported in scientific literature (Trachsel V et al).
Theoretical MW
54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Glut1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 7 Reviews rated 4.9
using
NB110-39113 in the following applications:

Publications
Read Publications using
NB110-39113 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 29456650). 100% sequence identity with primate, 93% sequence identity with bovine.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut1 Antibody

  • Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
  • CSE
  • DYT17
  • DYT18
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • Glut1
  • GLUT-1
  • GLUT1DS
  • HepG2 glucose transporter
  • HTLVR
  • Human T-Cell Leukemia Virus (I and II) Receptor
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • Solute Carrier Family 2 Member 1
  • solute carrier family 2, facilitated glucose transporter member 1

Background

Glucose transporter 1 (GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols transporters. GLUT proteins transport glucose across cellular membranes through facilitative mechanisms and play a key role in glucose homeostasis (1). Fourteen GLUT proteins have been identified in the human, which are encoded by SLC2A genes 1-14 and are broadly expressed in many cell types and tissues. GLUT family members differ in sequence homology, substrate specificity and expression patterns. Based on sequence homology, GLUT family members are classified into Class I (GLUT1, 2, 3, 4, and GLUT14), Class II (GLUT5, 7, 9, and 11), and Class III (GLUT6, 8, 10, 12 and 13) (1). Structurally, GLUT transporters are integral membrane glycoproteins consisting of 12 membrane spanning helical domains, a single N-linked glycosylation site, and having cytoplasmic facing carboxy and amino terminal domains (2).

GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).

References

1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. https://doi.org/10.1002/iub.315

2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine. https://doi.org/10.1016/j.mam.2012.07.001

3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps. https://doi.org/10.1016/b978-0-12-416579-3.00004-6

4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports. https://doi.org/10.1007/s11910-013-0342-7

5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences. https://doi.org/10.4103/JPN.JPN_169_17

6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice. https://doi.org/10.1016/j.prp.2017.04.023

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-39113
Species: Hu, Mu, Rt, Rb, Bv, Pm
Applications: WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro

Publications for Glut1 Antibody (NB110-39113)(33)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 8 applications: ChIP, FLOW, Flow, ICC/IF, IHC-Fr, IHC-P, In vitro, WB.


Filter By Application
ChIP
(2)
FLOW
(4)
Flow
(3)
ICC/IF
(2)
IHC-Fr
(2)
IHC-P
(1)
In vitro
(1)
WB
(17)
All Applications
Filter By Species
Human
(13)
Mouse
(12)
Rat
(3)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB110-39113 Applications Species
Li X, Wen MZ, Su LX et al. Local suture ligation-assisted percutaneous sclerotherapy for Kasabach-Merritt phenomenon-associated kaposiform haemangioendothelioma. Oncol Lett Jan 1 2019 [PMID: 30655857] (IHC-P, Human) IHC-P Human
Pacella I, Procaccini C, Focaccetti C et al. Fatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth Proc Proc Natl Acad Sci U S A. 2018 Jul 10 [PMID: 29941600] (Flow, Mouse)

Details:
Citation using the PE form of this antibody.
Flow Mouse
Kishore M, Cheung KCP, Fu H et al. Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis Immunity. 2017 Nov 21 [PMID: 29669254] (FLOW, Mouse)

Details:
The Phycoerythrin conjugated version of this antibody was used as part of this experiment, NB110-39113PE.
FLOW Mouse
Trachsel V In vitro fate mapping of stem cell development at the single cell level Thesis 2018 (FLOW, In vitro, Human)

Details:
The Phycoerythrin conjugated version of this antibody was used as part of this experiment, NB110-39113PE.
FLOW, In vitro Human
Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun May 29 2020 [PMID: 32472050] (WB, Human) WB Human
Baumann T, Dunkel A, Schmid C et al. Regulatory myeloid cells paralyze T cells through cell-cell transfer of the metabolite methylglyoxal Nat. Immunol. May 1 2020 [PMID: 32327756]
Galle-Treger L, Hurrell BP, Lewis G Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function J Allergy Clin Immunol. [PMID: 31738991] (Flow, Mouse) Flow Mouse
Bartel K, Muller R, von Schwarzenberg K Differential regulation of AMP-activated protein kinase in healthy and cancer cells explains why V-ATPase inhibition selectively kills cancer cells J Biol Chem. 2019 Nov 15 [PMID: 31604821] (WB, Human)

Details:
Citation used the HRP format of this antibody.
WB Human
McGuire JL, Correll EA, Lowery AC et al. Pioglitazone improves working memory performance when administered in chronic TBI Neurobiol. Dis. Sep 9 2019 [PMID: 31513844] (WB, Rat) WB Rat
Zhang J, Zhang Y, Liu Y et al. LAIR-1 overexpression inhibits osteosarcoma assets.researchsquare.com Jan 1 2020 (WB, Human) WB Human
Show All 33 Publications.

Reviews for Glut1 Antibody (NB110-39113) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Mouse and Human.

Reviews using NB110-39113:
Filter by Applications
WB
(5)
ICC
(2)
All Applications
Filter by Species
Human
(4)
Mouse
(2)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Glut1 NB110-39113
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reviewed by:
Anonymous
WB Mouse 04/23/2019
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Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotF-7
Immunocytochemistry Glut1 NB110-39113
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reviewed by:
Anonymous
ICC Human 10/24/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedbeta cells
SpeciesHuman
LotF-2

Comments

Commentsuse at 1:100
Western Blot Glut1 NB110-39113
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reviewed by:
Ajay Ashok
WB Human 06/12/2017
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Summary

ApplicationWestern Blot
Sample TestedBrain
SpeciesHuman

Comments

Commentsuse at 1:500
Immunocytochemistry Glut1 NB110-39113
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reviewed by:
Ajay Ashok
ICC Mouse 06/12/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse eye
SpeciesMouse

Comments

Commentsuse at 1:100 concentration
Western Blot Glut1 NB110-39113
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reviewed by:
Ajay Ashok
WB Mouse and Human 06/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedRetina (inner nuclear layer)
SpeciesMouse and Human

Comments

CommentsUse at a conc of 1:500 for 20ug sample protein loaded
Western Blot Glut1 NB110-39113
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reviewed by:
Marie-estelle LOSFELD
WB Human 03/09/2014
View

Summary

ApplicationWestern Blot
Sample Testedmembrane fraction from lung cancer cells
SpeciesHuman
Western Blot Glut1 NB110-39113
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reviewed by:
Anonymous
WB Human 09/01/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 20 ug
SpeciesHuman
CommentsWorks for human GLUT1

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:2000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit IgG-HRP, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 42 kDa

Comments

CommentsWorks for human GLUT1

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Glut1 Antibody (NB110-39113). (Showing 1 - 2 of 2 FAQs).

  1. I am looking for Glut1 antibody that could be used for flow cytometry and staining of live human cells.  
    • We recommend Glut1 antibody NB110-39113 for cell surface staining in flow cytometry on human cells (https://www.novusbio.com/products/glut1-antibody_nb110-39113). The immunogen for this product is between amino acid residues 1-100, and based on the UniProt topological information, it should bind to the extracellular region from 34-66 aa. This product has been validated in flow-intracellular, but based on the immunogen, should bind to the extracellular region as well.
  2. Can you tell me what, if any, antigen retrieval method was used for the staining of paraffin tissue sections with GLUT1 Antibody (NB110-39113)?
    • We recommend the use of HIER (heat-induced epitope retrieval) with 0.01 M sodium citrate buffer, pH 6.0 at 100C for 20 minutes in a steamer or pressure cooker.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-39113AF350
Alexa Fluor 405 NB110-39113AF405
Alexa Fluor 488 NB110-39113AF488
Alexa Fluor 532 NB110-39113AF532
Alexa Fluor 594 NB110-39113AF594
Alexa Fluor 647 NB110-39113AF647
Alexa Fluor 700 NB110-39113AF700
Alexa Fluor 750 NB110-39113AF750
Allophycocyanin/Cy7 NB110-39113APCCY7
Biotin NB110-39113B
DyLight 350 NB110-39113UV
DyLight 405 NB110-39113V
DyLight 488 NB110-39113G
DyLight 550 NB110-39113R
DyLight 594 NB110-39113DL594
DyLight 650 NB110-39113C
DyLight 680 NB110-39113FR
DyLight 755 NB110-39113IR
FITC NB110-39113F
HRP NB110-39113H
Janelia Fluor 549 NB110-39113JF549
Janelia Fluor 646 NB110-39113JF646
PE NB110-39113PE
PE/Atto594 NB110-39113PEATT594
PE/Cy5.5 NB110-39113PECY55
PE/Cy7 NB110-39113PECY7

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Blogs on Glut1.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

Anonymous
04/23/2019
Application: WB
Species: Mouse

Anonymous
10/24/2017
Application: ICC
Species: Human

Ajay Ashok
06/12/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC2A1