Western Blot: Glut1 Antibody [NB110-39113] - GLUT 1 in A549 cells. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Glut1 Antibody [NB110-39113] - GLUT1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Flow Cytometry: Glut1 Antibody [NB110-39113] - Analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture ...read more
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 0.5-2.0 ug/ml
This GLUT1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature.In WB a band is seen at ~55 kDa on kidney membrane preps representing GLUT1 protein. Depending on the tissue and any post-translational modifications, this protein can run anywhere between 40-60 kDa. The FLOW use of this antibody was submitted by a Verified Customer (See Review on NB110-39113PE for FLOW Protocol Details.
Read 2 Reviews rated 5 using NB110-39113 in the following applications:
Reacts with human and mouse. 100% sequence identity with primate protein, 93% sequence identity with cow, and rabbit proteins. Rat reactivity reported in scientific literature (PMID: 26590355)
Alternate Names for Glut1 Antibody
Glucose transporter type 1, erythrocyte/brain
HepG2 glucose transporter
solute carrier family 2 (facilitated glucose transporter), member 1
solute carrier family 2, facilitated glucose transporter member 1
GLUT1 is a major glucose transporter in the mammalian blood brain barrier. It is ubiquitous, and is present at high levels in primate erythrocytes and brain endothelial cells. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are seven types of glucose transport carrier proteins, designated as Glut 1 to 7.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Jacobs I, Hectors SJ, Schabel MC et al. Cluster analysis of DCE-MRI data identifies regional tracer-kinetic changes after tumor treatment with high intensity focused ultrasound NMR Biomed (IHC-Fr, Mouse)