Glut1 Antibody - BSA Free

Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY license.

Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).

Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.

Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective

Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza (PMID: 21620138).

Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.

Flow Cytometry: Glut1 Antibody [NB110-39113] - Flow cytometry analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. FMO control (red) and isotype control (blue, NBP2-24983) were compared to CD4+ T cells (orange), and this PE conjugated Glut 1 antibody positively stained CD4+ lymphocytes isolated from Mouse. Flow cytometry image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt, Rb, Bv, Pm
• Applications: WB, Simple Western, ChIP, Flow, Func, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Glut1 Antibody - BSA Free Summary

• Immunogen: This Glut1 antibody is made against a synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot# P11166]. The immunogen is cytosolic.
• Localization: Cell membrane, cytoplasm (near membranes), melanosome
• Marker: Plasma Membrane Marker
• Predicted Species: Primate (100%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature
  • Flow (Intracellular) 1 ug/ml
  • Flow Cytometry 1 ug/ml. Use reported by customer review
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200. Use reported in scientific literature
  • Immunohistochemistry-Paraffin 1:200
  • In vitro assay reported in scientific literature (Trachsel V et al)
  • Simple Western 1:50
  • Western Blot 1:500
• Application Notes: In WB a band is seen at ~55 kDa on kidney membrane preps representing GLUT1 protein. Depending on the tissue and any post-translational modifications, this protein can run anywhere between 40-60 kDa. Simple Western reported by an internal validation. Separated by Size, antibody dilution of 1:50
• Theoretical MW: 54.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 29456650). 100% sequence identity with primate, 93% sequence identity with bovine.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glut1 Antibody - BSA Free

• Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
• CSE
• DYT17
• DYT18
• DYT9
• EIG12
• Glucose transporter type 1, erythrocyte/brain
• Glut1
• GLUT-1
• GLUT1DS
• HepG2 glucose transporter
• HTLVR
• Human T-Cell Leukemia Virus (I and II) Receptor
• MGC141895
• MGC141896
• PED
• SLC2A1
• solute carrier family 2 (facilitated glucose transporter), member 1
• Solute Carrier Family 2 Member 1
• solute carrier family 2, facilitated glucose transporter member 1

Background

Glucose transporter 1 (GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols transporters. GLUT proteins transport glucose across cellular membranes through facilitative mechanisms and play a key role in glucose homeostasis (1). Fourteen GLUT proteins have been identified in the human, which are encoded by SLC2A genes 1-14 and are broadly expressed in many cell types and tissues. GLUT family members differ in sequence homology, substrate specificity and expression patterns. Based on sequence homology, GLUT family members are classified into Class I (GLUT1, 2, 3, 4, and GLUT14), Class II (GLUT5, 7, 9, and 11), and Class III (GLUT6, 8, 10, 12 and 13) (1). Structurally, GLUT transporters are integral membrane glycoproteins consisting of 12 membrane spanning helical domains, a single N-linked glycosylation site, and having cytoplasmic facing carboxy and amino terminal domains (2).

GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).

References

1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. https://doi.org/10.1002/iub.315

2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine. https://doi.org/10.1016/j.mam.2012.07.001

3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps. https://doi.org/10.1016/b978-0-12-416579-3.00004-6

4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports. https://doi.org/10.1007/s11910-013-0342-7

5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences. https://doi.org/10.4103/JPN.JPN_169_17

6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice. https://doi.org/10.1016/j.prp.2017.04.023

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut1 Antibody (NB110-39113)(53)

Publications using NB110-39113

• Artico LL, Ruas JS, Teixeira Júnior JR et al. IGFBP7 Fuels the Glycolytic Metabolism in B-Cell Precursor Acute Lymphoblastic Leukemia by Sustaining Activation of the IGF1R-Akt-GLUT1 Axis International journal of molecular sciences 2023-06-02 [PMID: 37298628] (FLOW)
• Yeudall S, Upchurch CM, Seegren PV et al. Macrophage acetyl-CoA carboxylase regulates acute inflammation through control of glucose and lipid metabolism Science advances 2022-11-25 [PMID: 36417534] (WB, Mouse)
  
  Details:
  Dilution of 1:1000 was used
• Li Q Toll-like receptor 2/7 activation enhances the metabolism and functions of CD8+ T and B cells Thesis 2020-01-01 (FLOW, PCR, Mouse)
• Pham K, Hanaford AR, Poore BA et al. Comprehensive Metabolic Profiling of MYC-Amplified Medulloblastoma Tumors Reveals Key Dependencies on Amino Acid, Tricarboxylic Acid and Hexosamine Pathways Cancers 2022-03-03 [PMID: 35267619] (WB, Human)
• García-Gómez P, Golán I, S Dadras M et al. NOX4 regulates TGF?-induced proliferation and self-renewal in glioblastoma stem cells Molecular oncology 2022-05-01 [PMID: 35203105] (WB, Human)
  
  Details:
  Multi-plex staining; Dilutions: 1:800
• Wang X, Hu J, Fang Y et al. Multi-Omics Profiling to Assess Signaling Changes upon VHL Restoration and Identify Putative VHL Substrates in Clear Cell Renal Cell Carcinoma Cell Lines Cells 2022-01-29 [PMID: 35159281] (WB, Human)
• Jaiswal AK, Yadav J, Makhija S Et al. Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging Am J Physiol Lung Cell Mol Physiol 2021-12-01 [PMID: 34851736] (FLOW, Mouse)
  
  Details:
  Citation using the PE version of this antibody.
• Silva JVV, Ganesan S, Wickramasinghe HKJP Et al. Effects of branched-chain amino acids on glucose uptake and lactose synthesis rates in bovine mammary epithelial cells and lactating mammary tissue slices Journal of dairy science 2021-11-18 [PMID: 34802743]
• Wessendarp M, Watanabe-Chailland M, Liu S Et al. Role of GM-CSF in regulating metabolism and mitochondrial functions critical to macrophage proliferation Mitochondrion 2021-11-01 [PMID: 34740864] (FLOW, Mouse)
• Pang L, Ng KT, Liu J et al. Plasmacytoid dendritic cells recruited by HIF-1 alpha /eADO/ADORA1 signaling induce immunosuppression in hepatocellular carcinoma Cancer letters 2021-09-16 [PMID: 34536555] (IF/IHC, Human)
• Shriwas P, Roberts D, Li Y et al. A small-molecule pan-class I glucose transporter inhibitor reduces cancer cell proliferation in vitro and tumor growth in vivo by targeting glucose-based metabolism Cancer & metabolism 2021-03-26 [PMID: 33771231] (IF/IHC)
• Abo El Gheit RE, Soliman NA, Badawi GA et al. Retinoprotective effect of agmatine in streptozotocin-induced diabetic rat model: avenues for vascular and neuronal protection : Agmatine in diabetic retinopathy Journal of physiology and biochemistry 2021-02-26 [PMID: 33635523] (IHC-P)
• Balukoff NC, Ho JJD, Theodoridis PR et al. A translational program that suppresses metabolism to shield the genome Autophagy 2020-11-08 [PMID: 33188200]
• Girotra M, Trachsel V, Roch A, Lutolf MP In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro Int J Mol Sci 2020-11-06 [PMID: 33153113] (Mouse)
  
  Details:
  Citation using the PE version of this antibody.
• Okazaki Y, Murray J, Ehsani A et al. Increased glucose metabolism in Arid5b-/- skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1) Biol Res 2020-10-06 [PMID: 33023658]
• Nesci V, Russo E, Arcidiacono B et al. Metabolic Alterations Predispose to Seizure Development in High-Fat Diet-Treated Mice: the Role of Metformin Mol. Neurobiol. 2020-08-12 [PMID: 32785826] (WB)
• Helou DG, Shafiei-Jahani P, Lo R et al. PD-1 pathway regulates ILC2 metabolism and PD-1 agonist treatment ameliorates airway hyperreactivity Nat Commun 2020-08-10 [PMID: 32778730] (Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• Trachsel V In vitro fate mapping of stem cell development at the single cell level Thesis 2018-01-01 (FLOW, In vitro, Human)
  
  Details:
  The Phycoerythrin conjugated version of this antibody was used as part of this experiment, NB110-39113PE.
• Jaiswal AK, Makhija S, Stahr N Et al. Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation Immunobiology 2020-08-05 [PMID: 32747016]
  
  Details:
  Citation using the PE version of this antibody.
• Cheung KCP, Fanti S, Mauro C et al. Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming Nat Commun 2020-07-17 [PMID: 32681081]
  
  Details:
  Citation using the PE/Cy7 format of this antibody.
• Zhang J, Zhang Y et al. LAIR-1 overexpression inhibits epithelial-mesenchymal transition in osteosarcoma via GLUT1-related energy metabolism. World J Surg Oncol 2020-06-20 [PMID: 32563267] (WB, ICC/IF, Human)
• Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun 2020-05-29 [PMID: 32472050] (WB, Human)
  
  Details:
  Citation using the HRP format of this antibody.
• Baumann T, Dunkel A, Schmid C et al. Regulatory myeloid cells paralyze T cells through cell-cell transfer of the metabolite methylglyoxal Nat. Immunol. 2020-05-01 [PMID: 32327756]
• Staricha K, Meyers N, Garvin J et al. Effect of high glucose condition on glucose metabolism in primary astrocytes Brain Res. 2020-02-04 [PMID: 32032612] (WB, Rat)
• Galle-Treger L, Hurrell BP, Lewis G Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function J Allergy Clin Immunol. [PMID: 31738991] (FLOW, Mouse)
• Bartel K, Muller R, von Schwarzenberg K Differential regulation of AMP-activated protein kinase in healthy and cancer cells explains why V-ATPase inhibition selectively kills cancer cells J Biol Chem. 2019-11-15 [PMID: 31604821] (WB, Human)
  
  Details:
  Citation used the HRP format of this antibody.
• McGuire JL, Correll EA, Lowery AC et al. Pioglitazone improves working memory performance when administered in chronic TBI Neurobiol. Dis. 2019-09-09 [PMID: 31513844] (WB, Rat)
• Li X, Wen MZ, Su LX et al. Local suture ligation-assisted percutaneous sclerotherapy for Kasabach-Merritt phenomenon-associated kaposiform haemangioendothelioma. Oncol Lett 2019-01-01 [PMID: 30655857] (IHC-P, Human)
• Appel S, Grothe J, Storck S et al. A potential role for GSK3beta in glucose-driven intrauterine catch-up growth in maternal obesity Endocrinology 2018-12-10 [PMID: 30535296] (Mouse)
• Patel S, Fu S, Mastio J et al. Unique pattern of neutrophil migration and function during tumor progression. Nat. Immunol. 2018-11-01 [PMID: 30323345] (FLOW, Mouse)
• Pacella I, Procaccini C, Focaccetti C et al. Fatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth Proc Proc Natl Acad Sci U S A. 2018-07-10 [PMID: 29941600] (FLOW, Mouse)
  
  Details:
  Citation using the PE form of this antibody.
• Ashok A, Singh N Prion protein modulates glucose homeostasis by altering intracellular iron Scientific Reports 2018-04-26 [PMID: 29700330] (WB)
• Kishore M, Cheung KCP, Fu H et al. Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis Immunity. 2017-11-21 [PMID: 29669254] (FLOW, Mouse)
  
  Details:
  The Phycoerythrin conjugated version of this antibody was used as part of this experiment, NB110-39113PE.
• Xu X, Cui N, Wang E. Application of an acellular dermal matrix to a rabbit model of oral mucosal defects Exp Ther Med 2018-01-05 [PMID: 29456650] (WB, Rabbit)
• Ho JJD, Balukoff NC, Cervantes G, Malcolm PD. Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B. Cell Rep. 2018-01-02 [PMID: 29298419] (WB)
• Thwe P, Pelgrom L, Cooper R et al. Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses. Cell Metab 2017 Sep 05 [PMID: 28877459] (FLOW, Mouse). 2017-09-05 [PMID: 28877459] (FLOW, Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 form of this antbody
• Wasser, Y, Y The Role of Dlk1 in the Gene Regulatory Network Underlying Motor Neuron Diversification Thesis 2022-01-01 (WB, Mouse)
• Trompette A, Gollwitzer ES, Pattaroni C et al. Dietary Fiber Confers Protection against Flu by Shaping Ly6c- Patrolling Monocyte Hematopoiesis and CD8+ T Cell Metabolism Immunity 2018-05-15 [PMID: 29768180] (Mouse)
• Cai TT, Ye SB, Liu YN et al. LMP1-mediated glycolysis induces myeloid-derived suppressor cell expansion in nasopharyngeal carcinoma PLoS Pathog. 2017-07-01 [PMID: 28732079] (ICC/IF, Human)
• Kuhnel E, Kleff V, Stojanovska V et al. PlacentalSpecific Overexpression of sFlt1 Alters Trophoblast Differentiation and Nutrient Transporter Expression in an IUGR Mouse Model. Journal of Cellular Biochemistry. 2016-11-09 [PMID: 27859593] (WB, Mouse)
• Nusken E, Gellhaus A, Kuhnel E et al. Increased Rat Placental Fatty Acid, But Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming. J. Cell. Biochem. 2015-11-21 [PMID: 26590355] (WB, Rat)
• Jacobs I, Hectors SJ, Schabel MC et al. Cluster analysis of DCE-MRI data identifies regional tracer-kinetic changes after tumor treatment with high intensity focused ultrasound. NMR Biomed. [PMID: 26390040] (IHC-Fr, Mouse)
  
  Details:
  Citation using the DyLight 650 form of this antibody.
• De Riccardis L, Rizzello A, Ferramosca A et al. Bioenergetics profile of CD4(+) T cells in relapsing remitting multiple sclerosis subjects J. Biotechnol. 2015-02-19 [PMID: 25701681] (WB, Human)
  
  Details:
  Glut1 antibody used for WB analysis on CD4+ T cells from control and MS patients at a dilution of 1:1,000 with overnight incubation (Fig. 2D).
• Tambe Y, Hasebe M, Kim CJ et al. The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B Mol. Carcinog. 2015-01-24 [PMID: 25620379] (WB, Mouse)
• Yang F, Lu W, Lin W et al. Enhancement of Neurotrophic Factors in Astrocyte for Neuroprotective Effects in Brain Disorders Using Low-intensity Pulsed Ultrasound Stimulation. Brain Stimul. 2014-12-04 [PMID: 25558041] (WB, Human)
• Rodriguez-Espinosa O, Rojas-Espinosa O, Moreno-Altamirano MM et al. Metabolic requirements for neutrophil extracellular traps nets) formation. Immunology 2014-12-27 [PMID: 25545227] (FLOW, Human)
  
  Details:
  Using the DyLight 650 conjugated version of NB110-39113, catalog number NB110-39113C.
• Takeshita Y, Obermeier B, Cotleur A et al. An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture. J. Neurosci. Methods. 2014-05-22 [PMID: 24858797] (ICC/IF, Human)
• Moreau JL, Artap ST, Shi H et al. Cited2 is required in trophoblasts for correct placental capillary patterning. Dev. Biol. 2014-05-05 [PMID: 24803182] (IHC-Fr, Mouse)
• Luo W, Chang R, Zhong J, Pandey A, Semenza GL. Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression. Proc Natl Acad Sci U S A. 2012-12-04 [PMID: 23129632] (Chemotaxis)
• Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, et al. Regulation of TFEB and V-ATPases by mTORC1 EMBO J 2011-07-01 [PMID: 21804531] (WB, Mouse)
• Luo W, Hu H, Chang R et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1. Cell;145(5):732-44. 2011-05-27 [PMID: 21620138] (Chemotaxis, WB, Human)
• Naga Prasad SV, Duan Z-H, Gupta MK et al. Unique MicroRNA Profile in End-stage Heart Failure Indicates Alterations in Specific Cardiovascular Signaling Networks. J Biol Chem;284(40):27487-27499. 2009-01-01 [PMID: 19641226] (WB, Human)
• Hergovich A et al. Priming-dependent phosphorylation and regulation of the tumor suppressor pVHL by glycogen synthase kinase 3. Mol Cell Biol;26(15):5784-96. 2006-08-01 [PMID: 16847331] (WB, Human)

Reviews for Glut1 Antibody (NB110-39113) (7)

Average Rating: 4.9

(Based on 7 reviews)

We have 7 reviews tested in 3 species: Human, Mouse, Human and Mouse.

reviewed by: Jaroslawna Meister WB Mouse 04/23/2019

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle
• Species: Mouse
• Lot: F-7

reviewed by: Ranjana Ravikumar ICC Human 10/24/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: beta cells
• Species: Human
• Lot: F-2

Comments

• Comments: use at 1:100

reviewed by: Verified Customer WB Human 06/12/2017

Summary

• Application: Western Blot
• Sample Tested: Brain
• Species: Human

Comments

• Comments: use at 1:500

reviewed by: Verified Customer ICC Mouse 06/12/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse eye
• Species: Mouse

Comments

• Comments: use at 1:100 concentration

reviewed by: Verified Customer WB Human and Mouse 06/12/2017

Summary

• Application: Western Blot
• Sample Tested: Retina (inner nuclear layer)
• Species: Human and Mouse

Comments

• Comments: Use at a conc of 1:500 for 20ug sample protein loaded

reviewed by: Verified Customer WB Human 03/09/2014

Summary

• Application: Western Blot
• Sample Tested: membrane fraction from lung cancer cells
• Species: Human

reviewed by: Weibo Luo WB Human 09/01/2010

Summary

• Application: Western Blot
• Sample Tested: HeLa cell lysates, Sample Amount: 20 ug
• Species: Human

FAQs for Glut1 Antibody (NB110-39113). (Showing 1 - 1 of 1 FAQs).

1. Can you tell me what, if any, antigen retrieval method was used for the staining of paraffin tissue sections with GLUT1 Antibody (NB110-39113)?
   • We recommend the use of HIER (heat-induced epitope retrieval) with 0.01 M sodium citrate buffer, pH 6.0 at 100C for 20 minutes in a steamer or pressure cooker.

Bioinformatics

• Gene Symbol: SLC2A1