Rabbit IgG Isotype Control

Images

 
Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] - Controls for alpha-MSH immunohistochemistry and immunofluorescence. (A) Isotype controls for alpha-MSH were obtained by staining consecutive sections of ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control, ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ChIP, Flow, ICC/IF, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Rabbit IgG Isotype Control Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG1
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32784470)ChIP usage was reported in scientific literature (PMID: 23034403). IHC-P usage was reported in scientific literature (PMID: 255994242). In vitro assay usage was reported in multiple scientific literatures with the Azide free version (NBP2-24893). Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24891 in the following applications:

Publications
Read Publications using
NBP2-24891 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Rabbit IgG Isotype Control

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for IgG Isotype Control (NBP2-24891)(39)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 7 applications: ChIP, Flow, ICC/IF, IHC, IHC-P, In vitro, WB.


Filter By Application
ChIP
(6)
Flow
(2)
ICC/IF
(7)
IHC
(9)
IHC-P
(2)
In vitro
(1)
WB
(1)
All Applications
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Human
(7)
Mouse
(5)
Rat
(1)
Bovine
(4)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using NBP2-24891 Applications Species
Dorschner BW, Wiedemuth R, Funke AC et al. Listening to the Whispers in Neuroimmune Crosstalk: A Comprehensive Workflow to Investigate Neurotrophin Receptor p75NTR Under Endogenous, Low Abundance Conditions Frontiers in Immunology Apr 16 2021 [PMID: 33936068]
Yoshimura T, Inokuchi Y, Mutou C et al. Age-related decline in the taurine content of the skin in rodents Amino acids Feb 20 2021 [PMID: 33608821] (IHC, Mouse)

Details:
Antibody used for negative controls
IHC Mouse
Qi X, Wang F, Chun C et al. A validated mouse model capable of recapitulating the protective effects of female sex hormones on ascending aortic aneurysms and dissections (AADs) Physiol Rep Nov 1 2020 [PMID: 33242364]
Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res Oct 19 2020 [PMID: 33091431] (ICC/IF) ICC/IF
Cayli S, Sahin C, Sanci TO, Nakkas H Inhibition of p97/VCP function leads to defective autophagosome maturation, cell cycle arrest and apoptosis in mouse Sertoli cells Theriogenology Sep 15 2020 [PMID: 32966945] (ICC/IF) ICC/IF
Mietus CJ, Lackner TJ, Karvelis PS et al. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med Aug 8 2020 [PMID: 32784470] (ICC/IF, Bovine, Human) ICC/IF Bovine, Human
Boontanrart MY, SchrOder MS, Stehli GM et al. ATF4 Regulates MYB to Increase gamma-Globin in Response to Loss of beta-Globin Cell Rep Jan 1 2020 [PMID: 32755585] (ChIP, Human) ChIP Human
Kumagai S, Togashi Y, Sakai C et al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells Immunity Jul 14 2020 [PMID: 32640259]

Details:
Citation using the PE format of this antibody.
Sakumoto R, Hayashi KG, Hosoe M et al. Pregnancy-associated changes of Peroxisome Proliferator-Activated Receptor Delta (PPARD) and Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) expression in the bovine corpus luteum J. Reprod. Dev. Feb 7 2020 [PMID: 32037375] (IHC, Bovine) IHC Bovine
Carpenter MD, Hu Q, Bond AM et al. Nr4a1 suppresses cocaine-induced behavior via epigenetic regulation of homeostatic target genes Nat Commun Jan 24 2020 [PMID: 31980629] (ChIP, Mouse) ChIP Mouse
Show All 39 Publications.

Review for IgG Isotype Control (NBP2-24891) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24891:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation IgG NBP2-24891
Enlarge
5
reviewed by:
Ranveer Singh
ChIP Human 06/18/2014
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCross-linked chromatin from HEK 293T cells
SpeciesHuman
CommentsRabbit IgG gave very less signal in ChIP and can be used as a control for pull-down

Primary Anitbody

Dilution Ratio2ug for 400 ul lysate

Details

Detection Notesreal time quantitative PCR (SYBR green)
Fixation/Cross-LinkingReverse cross linking was done in elution buffer with NaCl at 65 degree O/N
DNS SharingDiagenode Bioruptor was used in "High" setting, for 7 mins X 3 time to get approx 500bp fragments
Immunoprecipitation DetailsMagnetic Dynabeads (Protein G)
Crosslinking/Reversal Details1% formaldehyde for 10 mins at room temperature
DNA Prurification DetailsDNA was purified using PCR Purification Kit (Qiagen)

Comments

CommentsRabbit IgG gave very less signal in ChIP and can be used as a control for pull-down

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for IgG Isotype Control (NBP2-24891) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alexa Fluor 405 NBP2-24891AF405
Alexa Fluor 488 NBP2-24982
Alexa Fluor 594 NBP2-24891AF594
Alexa Fluor 647 NBP2-24891AF647
Alexa Fluor 700 NBP2-24891AF700
Allophycocyanin/Cy7 NBP2-24891APCCY7
DyLight 488 NBP2-24891G
FITC NBP2-24892
PE NBP2-24983

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

5
5
1
4
0
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0
2
0
1
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Ranveer Singh
06/18/2014
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol IGHG1