Rabbit IgG Isotype Control

Images

 
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control, ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ChIP, Flow, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Rabbit IgG Isotype Control Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG1
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
ChIP usage was reported in scientific literature (PMID: 23034403). IHC-P usage was reported in scientific literature (PMID: 255994242). In vitro assay usage was reported in multiple scientific literatures with the Azide free version (NBP2-24893). Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24891 in the following applications:

Publications
Read Publications using
NBP2-24891 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Rabbit IgG Isotype Control

  • IgG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (NBP2-24891)(29)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 7 applications: ChIP, Flow, ICC/IF, IHC, IHC-P, In vitro, WB.


Filter By Application
ChIP
(4)
Flow
(2)
ICC/IF
(4)
IHC
(7)
IHC-P
(2)
In vitro
(1)
WB
(1)
All Applications
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Human
(5)
Mouse
(3)
Rat
(1)
Bovine
(3)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NBP2-24891 Applications Species
Sakumoto R, Hayashi KG, Hosoe M et al. Pregnancy-associated changes of Peroxisome Proliferator-Activated Receptor Delta (PPARD) and Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) expression in the bovine corpus luteum J. Reprod. Dev. Feb 7 2020 [PMID: 32037375] (IHC, Bovine) IHC Bovine
Carpenter MD, Hu Q, Bond AM et al. Nr4a1 suppresses cocaine-induced behavior via epigenetic regulation of homeostatic target genes Nat Commun Jan 24 2020 [PMID: 31980629] (ChIP, Mouse) ChIP Mouse
Eustace NJ, Anderson JC, Langford CP et al. Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma. Int J Oncol. 2019 Mar 29 [PMID: 30942445] (WB, Rabbit)

Details:
Citation using the Azide Free form of this antibody.
WB Rabbit
Rinne P, Lyytikainen LP, Raitoharju E et al. Pro-opiomelanocortin and its Processing Enzymes Associate with Plaque Stability in Human Atherosclerosis- Tampere Vascular Study. Sci Rep. Oct 10 2018 [PMID: 30305673] (IHC-P, Mouse) IHC-P Mouse
Vazquez Rodriguez G, Abrahamsson A, Jensen LDE et al. Adipocytes Promote Early Steps of Breast Cancer Cell Dissemination via Interleukin-8 Front. Immunol 2018 Jul 30 [PMID: 30105032] (ICC/IF, Human)

Details:
This citation used the Azide Free version of this antibody.
ICC/IF Human
Sakurai T, kamio k, Sasaki k et al. Imaging Mass Microscopy of kidneys from Azithromycin-Treated Rats with Phospholipidosis. Am. J. Pathol. Jul 6 2018 [PMID: 29981744] (IHC, Rabbit) IHC Rabbit
Sakumoto R, Hayashi KG, Fujii S et al. Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy. Int J Mol Sci 2017 Mar 31 [PMID: 28362325] (IHC) IHC
Boontanrart MY, Stehli G, Banovic M, Schroeder M ATF4 mediates fetal globin upregulation in response to reduced beta-globin bioRxiv Jan 1 2020 (ChIP, Human) ChIP Human
Cavalli E, Fisch P, Formica F et al. A comparative study of cartilage engineered constructs in immunocompromised, humanized and immunocompetent mice Journal of Immunology and Regenerative Medicine Sep 1 2018 (IHC, Human) IHC Human
Hayashi KG, Hosoe M, Kizaki K et al. Differential gene expression profiling of endometrium during the mid-luteal phase of the estrous cycle between a repeat breeder (RB) and non-RB cows. Reprod. Biol. Endocrinol. 2017 Mar 23 [PMID: 28335821] (IHC, Bovine) IHC Bovine
Show All 29 Publications.

Review for IgG Isotype Control (NBP2-24891) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24891:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation IgG NBP2-24891
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reviewed by:
Ranveer Singh
ChIP Human 06/18/2014
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCross-linked chromatin from HEK 293T cells
SpeciesHuman
CommentsRabbit IgG gave very less signal in ChIP and can be used as a control for pull-down

Primary Anitbody

Dilution Ratio2ug for 400 ul lysate

Details

Detection Notesreal time quantitative PCR (SYBR green)
Fixation/Cross-LinkingReverse cross linking was done in elution buffer with NaCl at 65 degree O/N
DNS SharingDiagenode Bioruptor was used in "High" setting, for 7 mins X 3 time to get approx 500bp fragments
Immunoprecipitation DetailsMagnetic Dynabeads (Protein G)
Crosslinking/Reversal Details1% formaldehyde for 10 mins at room temperature
DNA Prurification DetailsDNA was purified using PCR Purification Kit (Qiagen)

Comments

CommentsRabbit IgG gave very less signal in ChIP and can be used as a control for pull-down

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Webinar
ChIP Video Protocol

FAQs for IgG Isotype Control (NBP2-24891) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alexa Fluor 405 NBP2-24891AF405
Alexa Fluor 488 NBP2-24982
Alexa Fluor 647 NBP2-24891AF647
Alexa Fluor 700 NBP2-24891AF700
Allophycocyanin/Cy7 NBP2-24891APCCY7
DyLight 488 NBP2-24891G
FITC NBP2-24892
PE NBP2-24983

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Recent Reviews

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1
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Ranveer Singh
06/18/2014
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol IGHG1
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