Rabbit IgG Isotype Control

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Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control, ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ChIP, Flow, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

Rabbit IgG Isotype Control Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG1
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
ChIP usage was reported in scientific literature (PMID: 23034403). IHC-P usage was reported in scientific literature (PMID: 255994242). In vitro assay usage was reported in multiple scientific literatures with the Azide free version (NBP2-24893). Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24891 in the following applications:

Publications
Read Publications using
NBP2-24891 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Alternate Names for Rabbit IgG Isotype Control

  • IgG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (NBP2-24891)(24)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 6 applications: ChIP, Flow, ICC/IF, IHC, IHC-P, In vitro.


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ChIP
(2)
Flow
(2)
ICC/IF
(4)
IHC
(5)
IHC-P
(2)
In vitro
(1)
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Human
(3)
Mouse
(2)
Rat
(1)
Bovine
(2)
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(1)
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Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NBP2-24891 Applications Species
Rinne P, Lyytikainen LP, Raitoharju E et al. Pro-opiomelanocortin and its Processing Enzymes Associate with Plaque Stability in Human Atherosclerosis- Tampere Vascular Study. Sci Rep. Oct 10 2018 [PMID: 30305673] (IHC-P, Mouse) IHC-P Mouse
Vazquez Rodriguez G, Abrahamsson A, Jensen LDE et al. Adipocytes Promote Early Steps of Breast Cancer Cell Dissemination via Interleukin-8 Front. Immunol 2018 Jul 30 [PMID: 30105032] (ICC/IF, Human)

Details:
This citation used the Azide Free version of this antibody.
ICC/IF Human
Sakurai T, kamio k, Sasaki k et al. Imaging Mass Microscopy of kidneys from Azithromycin-Treated Rats with Phospholipidosis. Am. J. Pathol. Jul 6 2018 [PMID: 29981744] (IHC, Rabbit) IHC Rabbit
Sakumoto R, Hayashi KG, Fujii S et al. Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy. Int J Mol Sci 2017 Mar 31 [PMID: 28362325] (IHC) IHC
Hayashi KG, Hosoe M, Kizaki K et al. Differential gene expression profiling of endometrium during the mid-luteal phase of the estrous cycle between a repeat breeder (RB) and non-RB cows. Reprod. Biol. Endocrinol. 2017 Mar 23 [PMID: 28335821] (IHC, Bovine) IHC Bovine
Subramanian K1, Bergman P, Henriques-Normark B. Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils. J Innate Immun. [PMID: 28241127] (Flow, Human)

Details:
Citation using the FITC form of this antibody.
Flow Human
Sareddy GR, Viswanadhapalli S, Surapaneni P et al. Novel KDM1A inhibitors induce differentiation and apoptosis of glioma stem cells via unfolded protein response pathway. Oncogene. Nov 28 2016 [PMID: 27893719]
Sakumoto R, Hayashi KG, Saito S et al. Comparison of the global gene expression profiles in the bovine endometrium between summer and autumn. J. Reprod. Dev. 2015 May 19 [PMID: 25994242] (IHC-P, Bovine) IHC-P Bovine
Nishimura Y, Takiguchi S, Ito S, Itoh K. EGF-stimulated AKT activation is mediated by EGFR recycling via an early endocytic pathway in a gefitinib-resistant human lung cancer cell line Int. J. Oncol. 2015 Apr 01 [PMID: 25653196]
Zimmermann M, Aguilera FB, Castellucci M et al. Chromatin remodelling and autocrine TNFalpha are required for optimal interleukin-6 expression in activated human neutrophils. Nat Commun. [PMID: 25616107] (Flow)

Details:
Citation using the PE form of this antibody.
Flow
Show All 24 Publications.

Review for IgG Isotype Control (NBP2-24891) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24891:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation IgG NBP2-24891
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reviewed by:
Ranveer Singh
ChIP Human 06/18/2014
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCross-linked chromatin from HEK 293T cells
SpeciesHuman
CommentsRabbit IgG gave very less signal in ChIP and can be used as a control for pull-down

Primary Anitbody

Dilution Ratio2ug for 400 ul lysate

Details

Detection Notesreal time quantitative PCR (SYBR green)
Fixation/Cross-LinkingReverse cross linking was done in elution buffer with NaCl at 65 degree O/N
DNS SharingDiagenode Bioruptor was used in "High" setting, for 7 mins X 3 time to get approx 500bp fragments
Immunoprecipitation DetailsMagnetic Dynabeads (Protein G)
Crosslinking/Reversal Details1% formaldehyde for 10 mins at room temperature
DNA Prurification DetailsDNA was purified using PCR Purification Kit (Qiagen)

Comments

CommentsRabbit IgG gave very less signal in ChIP and can be used as a control for pull-down

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar

FAQs for IgG Isotype Control (NBP2-24891) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

DyLight 488 Labeled NBP2-24891G

Additional Array Products

Bioinformatics Tool for IgG Isotype Control (NBP2-24891)

Discover related pathways, diseases and genes to IgG Isotype Control (NBP2-24891). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on IgG.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

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1
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Ranveer Singh
06/18/2014
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol IGHG1