Rabbit IgG Isotype Control

Images

 
Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] - Controls for alpha-MSH immunohistochemistry and immunofluorescence. (A) Isotype controls for alpha-MSH were obtained by staining consecutive sections of ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control , ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ChIP, Flow, ICC/IF, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Rabbit IgG Isotype Control Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG1
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • In vitro assay
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32784470)ChIP usage was reported in scientific literature (PMID: 23034403). IHC-P usage was reported in scientific literature (PMID: 255994242). In vitro assay usage was reported in multiple scientific literatures with the Azide free version (NBP2-24893). Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24891 in the following applications:

Publications
Read Publications using
NBP2-24891 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Rabbit IgG Isotype Control

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (NBP2-24891)(45)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 7 applications: ChIP, Flow, ICC/IF, IHC, IHC-P, In vitro, WB.


Filter By Application
ChIP
(6)
Flow
(3)
ICC/IF
(7)
IHC
(10)
IHC-P
(3)
In vitro
(1)
WB
(2)
All Applications
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Human
(7)
Mouse
(5)
Rat
(1)
Bovine
(4)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using NBP2-24891 Applications Species
Thomason EJ, SuArez-Pozos E, Afshari FS et al. Deletion of the Sodium-Dependent Glutamate Transporter GLT-1 in Maturing Oligodendrocytes Attenuates Myelination of Callosal Axons During a Postnatal Phase of Central Nervous System Development Frontiers in cellular neuroscience Jun 3 2022 [PMID: 35722615] (Flow) Flow
Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res Oct 19 2020 [PMID: 33091431] (ICC/IF) ICC/IF
Cayli S, Sahin C, Sanci TO, Nakkas H Inhibition of p97/VCP function leads to defective autophagosome maturation, cell cycle arrest and apoptosis in mouse Sertoli cells Theriogenology Sep 15 2020 [PMID: 32966945] (ICC/IF) ICC/IF
Kumagai S, Togashi Y, Sakai C et al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells Immunity Jul 14 2020 [PMID: 32640259]

Details:
Citation using the PE format of this antibody.
Carpenter MD, Hu Q, Bond AM et al. Nr4a1 suppresses cocaine-induced behavior via epigenetic regulation of homeostatic target genes Nat Commun Jan 24 2020 [PMID: 31980629] (ChIP, Mouse) ChIP Mouse
Boontanrart MY, Stehli G, Banovic M, Schroeder M ATF4 mediates fetal globin upregulation in response to reduced beta-globin bioRxiv Jan 1 2020 (ChIP, Human) ChIP Human
Mietus CJ, Lackner TJ, Karvelis PS et al. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med Aug 8 2020 [PMID: 32784470] (ICC/IF, Bovine, Human) ICC/IF Bovine, Human
Boontanrart MY, SchrOder MS, Stehli GM et al. ATF4 Regulates MYB to Increase gamma-Globin in Response to Loss of beta-Globin Cell Rep Jan 1 2020 [PMID: 32755585] (ChIP, Human) ChIP Human
Sakumoto R, Hayashi KG, Hosoe M et al. Pregnancy-associated changes of Peroxisome Proliferator-Activated Receptor Delta (PPARD) and Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) expression in the bovine corpus luteum J. Reprod. Dev. Feb 7 2020 [PMID: 32037375] (IHC, Bovine) IHC Bovine
Zhou G, Liao M et al. Cyclophilin A contributes to aortopathy induced by postnatal loss of smooth muscle TGFBR1. FASEB J Jan 10 2019 [PMID: 31311317] (IHC, Mouse) IHC Mouse
Show All 45 Publications.

Review for IgG Isotype Control (NBP2-24891) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24891:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation IgG NBP2-24891
Enlarge
5
reviewed by:
Verified Customer
ChIP Human 06/18/2014
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCross-linked chromatin from HEK 293T cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for IgG Isotype Control (NBP2-24891) (0)

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
5
1
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2
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1
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Verified Customer
06/18/2014
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol IGHG1