Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] - Controls for alpha-MSH immunohistochemistry and immunofluorescence. (A) Isotype controls for alpha-MSH were obtained by staining consecutive sections of ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control , ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 ...read more
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose ...read more
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 23034403)
Flow Cytometry 1:10 - 1:1000
Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 32784470)
Immunohistochemistry-Paraffin 1:10 - 1:500. Use reported in scientific literature (PMID 255994242)
In vitro assay
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. In vitro assay reported in multiple scientific literatures with the Azide free version (NBP2-24893)
Read 1 Review rated 5 using NBP2-24891 in the following applications:
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
Cavalli E, Fisch P, Formica F et al. A comparative study of cartilage engineered constructs in immunocompromised, humanized and immunocompetent mice Journal of Immunology and Regenerative Medicine 2018-09-01 (IF/IHC, Human)
Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.