Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
This Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] was developed against rabbit IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:50000
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:200 - 1:5000
Application Notes
Use in Immunohistochemistry-Paraffin was reported in the scientific literature (PMID: 20714769).
Readout System
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB7160 in the following applications:

Publications
Read Publications using
NB7160 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7160)(20)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 3 applications: ELISA, IHC-P, WB.


Filter By Application
ELISA
(2)
IHC-P
(1)
WB
(9)
All Applications
Filter By Species
Rat
(2)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB7160 Applications Species
Mehta N, Maddineni S, Mathews II et al. Structure and Functional Binding Epitope of V-domain Ig Suppressor of T Cell Activation Cell Rep Sep 3 2019 [PMID: 31484064] (ELISA) ELISA
Gunasekar P, Satish M, Dabestani P et al. Modulation of Cathepsin L Expression in the Coronary Arteries of Atherosclerotic Swine J Surg Res Aug 5 2019 [PMID: 31377485] (WB) WB
Elmahmoudy B, Abd El Fattah M, Elyamany M et al. Pyrrolidine dithiocarbamate and saxagliptin ameliorate ulcerative colitis in rats Asian Pac J Trop Biomed 2019 Oct 01 (WB, Rat) WB Rat
Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun May 29 2020 [PMID: 32472050] (WB) WB
Mehta NK Investigation of VISTA as a Cancer Immune Checkpoint Via Structural Analysis and Therapeutic Antibody Development Thesis Jan 1 2020
Harb I, Ashour H, Sabry D et al. Nicorandil prevents the nephrotoxic effect of cyclosporine-A in albino rats through modulation of HIF-1alpha/VEGF /eNOS signaling Can. J. Physiol. Pharmacol. Aug 21 2020 [PMID: 32822562]
Kaji S, Maki T, Ueda J et al. BCAS1-positive immature oligodendrocytes are affected by the alpha-synuclein-induced pathology of multiple system atrophy Acta Neuropathol Commun Jul 29 2020 [PMID: 32727582] (WB, Rat) WB Rat
La Fuente JM, Sevilleja-Ortiz A, Garcia-Rojo E et al. Erectile dysfunction is associated with defective L-cysteine/hydrogen sulfide pathway in human corpus cavernosum and penile arteries Eur. J. Pharmacol. Jul 23 2020 [PMID: 32712093] (WB) WB
AbuBakr N, Haggag T, Sabry D et al. Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGF beta/ Smad3 signaling pathway Heliyon May 9 2020 [PMID: 32382678] (WB) WB
Grillo SL, McDevitt DS, Voas MG et al. Adenosine receptor expression in the adult zebrafish retina Purinergic Signal Jul 6 2019 [PMID: 31273575] (WB) WB
Show All 20 Publications.

Reviews for IgG (H+L) Antibody (NB7160) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Human, Duck, Turkey, yeast.

Reviews using NB7160:
Filter by Applications
ELISA
(2)
WB
(2)
All Applications
Filter by Species
Human
(1)
Duck
(1)
Turkey
(1)
yeast
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Duck 02/14/2018
View

Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesDuck
LotP40
ELISA IgG (H+L) NB7160
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reviewed by:
Revathi Shanmugasundaram
ELISA Turkey 02/06/2018
View

Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesTurkey
LotP40
Western Blot IgG (H+L) NB7160
Enlarge
reviewed by:
Anonymous
WB yeast 12/05/2017
View

Summary

ApplicationWestern Blot
Sample Testedp-38
Speciesyeast
LotP40
Western Blot IgG (H+L) NB7160
Enlarge
reviewed by:
Anonymous
WB Human 09/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB7160
Secondary ConcentrationDiluted 20,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 1 min exposure on X-ray film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7160). (Showing 1 - 1 of 1 FAQs).

  1. We recently purchased your Acetylcholinesterase/ACHE Antibody (NBP1-31329) and Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (NB7160) for western blot. What buffer do you recommend for diluting these particular antibodies and what is the recommended blocking buffer?
    • Acetylcholinesterase/ACHE Antibody #NBP1-31329 is already in PBS and 20% glycerol.  Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] #NB7160 is in PBS and 0.2% BSA.  You can use PBS for diluting either one of them. We do not have a specific protocol for these antibodies. Our standard in house blocking buffer is TBST with 5% non-fat dry milk. You may refer to our general protocol for any more details or tips:   https://www.novusbio.com/support/support-by-application/western-blot/protocol.html

Other Available Formats

Alkaline Phosphatase NB7157
Biotin NB7158
FITC NB7159
HRP NB7160
PerCP NB7156PCP

Additional IgG (H+L) Products

Array NB7160

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

 
Anonymous
02/14/2018
Application: ELISA
Species: Duck

Revathi Shanmugasundaram
02/06/2018
Application: ELISA
Species: Turkey

Anonymous
12/05/2017
Application: WB
Species: yeast