Goat anti-Rabbit IgG (H L) Secondary Antibody [HRP]

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Rabbit IgG-heavy and light chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:50000
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:200 - 1:5000
Readout System
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB7160 in the following applications:

Publications
Read Publications using
NB7160 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.1% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7160)(10)

We have publications tested in 2 confirmed species: Porcine, Rabbit.

We have publications tested in 3 applications: ELISA, IHC-P, WB.


Filter By Application
ELISA
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Porcine
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB7160 Applications Species
Almalki SG, Llamas Valle Y, Agrawal DK. MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. Stem Cells Transl Med. 2017 Feb 18 [PMID: 28213979] (WB, Porcine) WB Porcine
Bigot P, Colli LM, Machiela MJ et al. Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun 2016 Jul 07 [PMID: 27384883] (WB) WB
Hur J, Kim CS, Eo SK et al. Salmonella ghosts expressing enterotoxigenic Escherichia coli k88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model Vet Q 2015 Apr 08 [PMID: 25853619] (ELISA) ELISA
Fujii S, Hara H, Araya J et al. Insufficient autophagy promotes bronchial epithelial cell senescence in chronic obstructive pulmonary disease. Oncoimmunology 2012 Aug [PMID: 22934255]
Lu Y, Kim C, Collins JF et al. Multiple Menkes copper ATPase (Atp7a) transcript and protein variants are induced by iron deficiency in rat duodenal enterocytes. J Trace Elem Med Biol 2012 Jun [PMID: 22579041]
Ganburged G, Suda N, Saito M et al. Dilated capillaries, disorganized collagen fibers and differential gene expression in periodontal ligaments of hypomorphic fibrillin-1 mice. Cell Tissue Res 2010 Sep [PMID: 20714769] (IHC-P, Rabbit) IHC-P Rabbit
Dickinson GH, Vega IE, Wahl KJ et al. Barnacle cement: a polymerization model based on evolutionary concepts. J Exp Biol;212(Pt 21):3499-510. 2009 Nov. [PMID: 19837892]
Wang H, Kubica N, Ellisen LW et al. Dexamethasone represses signaling through the mammalian target of rapamycin in muscle cells by enhancing expression of REDD1. J Biol Chem 2006 Dec [PMID: 17074751]
Kimball SR, Siegfried BA, Jefferson LS et al. Glucagon represses signaling through the mammalian target of rapamycin in rat liver by activating AMP-activated protein kinase. J Biol Chem 2004 Dec [PMID: 15494402]
Roesler U, Scholz H, Hensel A et al. Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identification. Int J Syst Evol Microbiol 2003 Jul [PMID: 12892149]

Reviews for IgG (H+L) Antibody (NB7160) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Human, Duck, Turkey, yeast.

Reviews using NB7160:
Filter by Applications
ELISA
(2)
WB
(2)
All Applications
Filter by Species
Human
(1)
Duck
(1)
Turkey
(1)
yeast
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Duck 02/14/2018
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Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesDuck
LotP40
ELISA IgG (H+L) NB7160
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reviewed by:
Revathi Shanmugasundaram
ELISA Turkey 02/06/2018
View

Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesTurkey
LotP40
Western Blot IgG (H+L) NB7160
Enlarge
reviewed by:
Anonymous
WB yeast 12/05/2017
View

Summary

ApplicationWestern Blot
Sample Testedp-38
Speciesyeast
LotP40
Western Blot IgG (H+L) NB7160
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reviewed by:
Anonymous
WB Human 09/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB7160
Secondary ConcentrationDiluted 20,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 1 min exposure on X-ray film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7160) (0)

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

 
Anonymous
02/14/2018
Application: ELISA
Species: Duck

Revathi Shanmugasundaram
02/06/2018
Application: ELISA
Species: Turkey

Anonymous
12/05/2017
Application: WB
Species: yeast