Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
This Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] was developed against rabbit IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:5000 - 1:50000
Readout System
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB7160 in the following applications:

Publications
Read Publications using
NB7160 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • 3 publications
  • WB
    18 publications

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS, 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7160)(35)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 4 applications: ELISA, IHC, IHC-P, WB.


Filter By Application
ELISA
(2)
IHC
(1)
IHC-P
(3)
WB
(18)
All Applications
Filter By Species
Rat
(1)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using NB7160 Applications Species
Kortam MA, Alawady AS, Hamid Sadik NA, Fathy N Fenofibrate mitigates testosterone induced benign prostatic hyperplasia via regulation of Akt/FOXO3a pathway and modulation of apoptosis and proliferation in rats Archives of biochemistry and biophysics [PMID: 35430215] (WB) WB
Mehta NK Investigation of VISTA as a Cancer Immune Checkpoint Via Structural Analysis and Therapeutic Antibody Development Thesis Jan 1 2020
Swamy G, Salo P, Duncan N et al. IL-1Ra deficiency accelerates intervertebral disc degeneration in C57BL6J mice JOR Spine [PMID: 35783913] (IHC-P) IHC-P
Salem MA, Manaa EG, Osama N et al. Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFbeta/SMAD pathway Scientific reports [PMID: 35449437] (WB) WB
Wu X, Pang Q, Zhi F et al. Overexpression of Long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells Tissue and Cell Feb 1 2022 [PMID: 35325674] (WB) WB
Karim S, Burzangi AS, Ahmad A et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer International journal of molecular sciences Feb 19 2022 [PMID: 35216429]
Abdel-Latif R, Wadie W, Abdel-mottaleb Y et al. Reposition of the anti-inflammatory drug diacerein in an in-vivo colorectal cancer model Saudi Pharmaceutical Journal Dec 1 2021 [PMID: 35145347] (WB) WB
Marchini M, Ashkin MR, Bellini M et al. A Na+/K+ ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice Frontiers in cell and developmental biology Dec 14 2021 [PMID: 34970538] (IHC) IHC
Sevilleja-Ortiz A, El Assar M, GarcIa-Rojo E Et al. Ageing-induced hypercontractility is related to functional enhancement of STIM/Orai and upregulation of Orai 3 in rat and human penile tissue Mechanisms of ageing and development Oct 23 2021 [PMID: 34699858] (WB) WB
Chen F, Liu J, Song X Et al. EZH2 inhibition confers PIK3CA-driven lung tumors enhanced sensitivity to PI3K inhibition Cancer letters Oct 13 2021 [PMID: 34655667] (WB) WB
Show All 35 Publications.

Reviews for IgG (H+L) Antibody (NB7160) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Human, Duck, Turkey, Yeast.

Reviews using NB7160:
Filter by Applications
ELISA
(2)
Western Blot
(2)
All Applications
Filter by Species
Human
(1)
Duck
(1)
Turkey
(1)
Yeast
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Lisa Ikariyama
ELISA Duck 02/14/2018
View

Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesDuck
LotP40
ELISA IgG (H+L) NB7160
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5
reviewed by:
Verified Customer
ELISA Turkey 02/06/2018
View

Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesTurkey
LotP40
Western Blot IgG (H+L) NB7160
Enlarge
4
reviewed by:
hisashi iwata
Western Blot Yeast 12/05/2017
View

Summary

ApplicationWestern Blot
Sample Testedp-38
SpeciesYeast
LotP40
Western Blot IgG (H+L) NB7160
Enlarge
5
reviewed by:
James Crocket
Western Blot Human 09/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7160). (Showing 1 - 1 of 1 FAQs).

  1. We recently purchased your Acetylcholinesterase/ACHE Antibody (NBP1-31329) and Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (NB7160) for western blot. What buffer do you recommend for diluting these particular antibodies and what is the recommended blocking buffer?
    • Acetylcholinesterase/ACHE Antibody #NBP1-31329 is already in PBS and 20% glycerol.  Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] #NB7160 is in PBS and 0.2% BSA.  You can use PBS for diluting either one of them. We do not have a specific protocol for these antibodies. Our standard in house blocking buffer is TBST with 5% non-fat dry milk. You may refer to our general protocol for any more details or tips:   https://www.novusbio.com/support/support-by-application/western-blot/protocol.html

Additional IgG (H+L) Products

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

 
Lisa Ikariyama
02/14/2018
Application: ELISA
Species: Duck

Verified Customer
02/06/2018
Application: ELISA
Species: Turkey

hisashi iwata
12/05/2017
Application: Western Blot
Species: Yeast