Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
This Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] was developed against rabbit IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:5000 - 1:50000
Readout System
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB7160 in the following applications:

Publications
Read Publications using NB7160.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS, 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7160)(62)

We have publications tested in 7 applications: B/N, ELISA, Func, IF/IHC, IHC-P, In Vivo, WB.


Filter By Application
B/N
(3)
ELISA
(2)
Func
(1)
IF/IHC
(1)
IHC-P
(4)
In Vivo
(1)
WB
(32)
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using NB7160 Applications Species
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P)

Details:
Citation using the HRP version of this antibody.
IHC-P
Ji Y, Liang Y, Chu PH Et al. The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice Biomed J 2022-10-16 [PMID: 36244649] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Swamy G, Salo P, Duncan N Et al. IL-1Ra deficiency accelerates intervertebral disc degeneration in C57BL6J mice JOR Spine 2022-07-05 [PMID: 35783913] (IHC-P)

Details:
Citation using the HRP version of this antibody.
IHC-P
Hendawy AS, El-Lakkany NM, Mantawy EM Et al. Vildagliptin alleviates liver fibrosis in NASH diabetic rats via modulation of insulin resistance, oxidative stress, and inflammatory cascades Life Sci 2022-06-07 [PMID: 35671811] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Salem MA, Manaa EG, Osama N Et al. Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFb/SMAD pathway Sci Rep 2022-04-22 [PMID: 35449437] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Kortam MA, Alawady AS, Hamid Sadik NA Et al. Fenofibrate mitigates testosterone induced benign prostatic hyperplasia via regulation of Akt/FOXO3a pathway and modulation of apoptosis and proliferation in rats Arch Biochem Biophys 2022-04-17 [PMID: 35430215] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Wu X, Pang Q, Zhi F Et al. Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells Tissue Cell 2022-03-24 [PMID: 35325674] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Karim S, Burzangi AS, Ahmad A Et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer Int J Mol Sci 2022-02-26 [PMID: 35216429]

Details:
Citation using the HRP version of this antibody.
Abdel-Latif RT, Wadie W, Abdel-Mottaleb Y Et al. Reposition of the anti-inflammatory drug diacerein in an in-vivo colorectal cancer model Saudi Pharm J 2022-02-11 [PMID: 35145347] (WB)

Details:
Citation using the HRP version of this antibody.
WB
Marchini M, Ashkin MR, Bellini M Et al. A Na(+)/K(+) ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice Front Cell Dev Biol 2021-12-31 [PMID: 34970538] (IF/IHC)

Details:
Citation using the HRP version of this antibody.
IF/IHC
Show All 62 Publications.

Reviews for IgG (H+L) Antibody (NB7160) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Human, Duck, Turkey, Yeast.

Reviews using NB7160:
Filter by Applications
ELISA
(2)
WB
(2)
All Applications
Filter by Species
Human
(1)
Duck
(1)
Turkey
(1)
Yeast
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ELISA Duck 02/14/2018
View

Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesDuck
LotP40
ELISA IgG (H+L) NB7160
Enlarge
5
reviewed by:
Revathi Shanmugasundaram
ELISA Turkey 02/06/2018
View

Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesTurkey
LotP40
Western Blot IgG (H+L) NB7160
Enlarge
4
reviewed by:
Verified Customer
WB Yeast 12/05/2017
View

Summary

ApplicationWestern Blot
Sample Testedp-38
SpeciesYeast
LotP40
Western Blot IgG (H+L) NB7160
Enlarge
5
reviewed by:
Verified Customer
WB Human 09/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7160). (Showing 1 - 1 of 1 FAQs).

  1. We recently purchased your Acetylcholinesterase/ACHE Antibody (NBP1-31329) and Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (NB7160) for western blot. What buffer do you recommend for diluting these particular antibodies and what is the recommended blocking buffer?
    • Acetylcholinesterase/ACHE Antibody #NBP1-31329 is already in PBS and 20% glycerol.  Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] #NB7160 is in PBS and 0.2% BSA.  You can use PBS for diluting either one of them. We do not have a specific protocol for these antibodies. Our standard in house blocking buffer is TBST with 5% non-fat dry milk. You may refer to our general protocol for any more details or tips.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

 
Verified Customer
02/14/2018
Application: ELISA
Species: Duck

Revathi Shanmugasundaram
02/06/2018
Application: ELISA
Species: Turkey

Verified Customer
12/05/2017
Application: WB
Species: Yeast