Human VEGF Quantikine ELISA Kit

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Reactivity HuSpecies Glossary
Applications ELISA

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Human VEGF Quantikine ELISA Kit Summary

The Quantikine Human VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF165 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF and recombinant human VEGF121 showed linear that were parallel to the standard curves obtained using the Quantikine Human VEGF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF.
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Natural and recombinant human VEGF. This assay also recognizes recombinant human VEGF165b.
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
Interference observed with 1 or more available related molecules.
Read Publications using DVE00.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human VEGF Quantikine ELISA Kit

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for VEGF (DVE00)(508)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Canine, Feline, Guinea Pig, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca fascicularis (Cynomolgus), Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transfected, Xenograft.

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Guinea Pig
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca fascicularis (Cynomolgus)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 508. Show All 508 Publications.
Publications using DVE00 Applications Species
K Sano, M Usui, Y Moritani, K Nakazawa, T Hanatani, H Kondo, M Nakatomi, S Onizuka, T Iwata, T Sato, A Togari, W Ariyoshi, T Nishihara, K Nakashima Co-cultured spheroids of human periodontal ligament mesenchymal stem cells and vascular endothelial cells enhance periodontal tissue regeneration Regen Ther, 2020;14(0):59-71. 2020 [PMID: 31988996] (Human) Human
H Bi, FM Aldakheel, M Sawsan Alo The effect of prednisolone on endometrial VGEF concentrations, Gene polymorphisms and pregnancy outcome in women with polycystic ovary syndrome: A retrospective cohort study J. Reprod. Immunol., 2020;137(0):103081. 2020 [PMID: 31978749] (Human) Human
J Doll, A Moghaddam, V Daniel, B Biglari, R Heller, G Schmidmaie, TF Raven LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy J Orthop, 2020;17(0):208-214. 2020 [PMID: 31889743] (Human) Human
S Kajiwara, K Ishii, T Sasaki, M Kato, K Nishikawa, H Kanda, K Arima, M Watanabe, Y Sugimura Castration-induced stromal remodeling disrupts the reconstituted prostate epithelial structure Lab. Invest., 2019;0(0):. 2019 [PMID: 31857695] (Human) Human
A Vijayan, S A, GSV Kumar PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing Sci Rep, 2019;9(1):19165. 2019 [PMID: 31844069] (Human) Human
Y Zhao, W Xiao, K Chen, Q Zhan, F Ye, X Tang, X Zhang Neurocognition and social cognition in remitted first-episode schizophrenia: correlation with VEGF serum levels BMC Psychiatry, 2019;19(1):403. 2019 [PMID: 31842818] (Human) Human
DY Kim, SS Lee, YK Bae Colorectal cancer cells differentially impact migration and microRNA expression in endothelial cells Oncol Lett, 2019;18(6):6361-6370. 2019 [PMID: 31814846] (Human) Human
D Hao, C He, B Ma, L Lankford, L Reynaga, DL Farmer, F Guo, A Wang Hypoxic Preconditioning Enhances Survival and Proangiogenic Capacity of Human First Trimester Chorionic Villus-Derived Mesenchymal Stem Cells for Fetal Tissue Engineering Stem Cells Int, 2019;2019(0):9695239. 2019 [PMID: 31781252] (Human) Human
R Takeuchi, W Katagiri, S Endo, T Kobayashi Exosomes from conditioned media of bone marrow-derived mesenchymal stem cells promote bone regeneration by enhancing angiogenesis PLoS ONE, 2019;14(11):e0225472. 2019 [PMID: 31751396] (Human) Human
M Hatakeyama, M Kanazawa, I Ninomiya, K Omae, Y Kimura, T Takahashi, O Onodera, M Fukushima, T Shimohata A novel therapeutic approach using peripheral blood mononuclear cells preconditioned by oxygen-glucose deprivation Sci Rep, 2019;9(1):16819. 2019 [PMID: 31728010] (Human) Human
Show All 508 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF (DVE00). (Showing 1 - 2 of 2 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Blogs on VEGF. Showing 1-10 of 21 blog posts - Show all blog posts.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Showing 1-10 of 21 blog posts - Show all blog posts.
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Gene Symbol VEGFA