EGLN1/PHD2 Antibody

Images

 
Genetic Strategies: Knockout Validated: EGLN1/PHD2 Antibody [NB100-137] - Immunoblot validation of HIF2A and PHD2 KO clones using HIF2A (#NB100-122; dilution: 1/300), PHD2 (#NB100-137; dilution: 1/500). To blot ...read more
Simple Western: EGLN1/PHD2 Antibody [NB100-137] - Simple Western lane view shows a specific band for PHD2/HIF Prolyl Hydroxylase 2 in 0.5 mg/mL of hypoxic HeLa cell lysate. This experiment was performed under reducing ...read more
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 expression in human prostate cancer cell lines. Western blot image submitted by a verified customer review.
Genetic Strategies: Knockdown Validated: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2 in human glioblastoma cell lysates. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of endogenous PHD2 in U2OS cells. ICC/IF image submitted by a verified customer review.
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of human PHD2 by Western blot. Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells. EGLN1/PHD2 antibody used at 1 ...read more
Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-137] - EGLN1/PHD2 and EGFR expression levels positively correlate in breast cancer. Processed tissue microarrays of breast cancer biopsies from 313 patients were stained ...read more
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Exogenous PHD2 has no impact on the gene expression of HIF-1alpha and HIF-2alpha. U87MG cells were transfected with empty vector (pcDNA) or PHD2 plasmid. Twenty-four hours ...read more
Flow Cytometry: EGLN1/PHD2 Antibody [NB100-137] - An intracellular stain was performed on Jurkat cells with NB100-137AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in normoxia (lane 1) or hypoxia (lane 2) treated SK-N-BE(2) cells using anti-EGLN1/PHD2 antibody. Western blot image submitted by a verified customer ...read more
Immunocytochemistry/ Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of PHD2 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-137] - Staining of lung vascular endothelium with EGLN1/PHD2 antibody. Image at 40X.
Flow Cytometry: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2 in Jurkat cells. One million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/mL anti-EGLN1/PHD2 antibody in a 150 uL reaction. Isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Pm-Or, Rb, RMSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

EGLN1/PHD2 Antibody Summary

Immunogen
The epitope recognized by this EGLN1/PHD2 antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
Localization
Cytoplasm, nucleus
Predicted Species
Orangutan (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 3.0 mcg/mL
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Simple Western 1:500
  • Western Blot 1:500 - 1:2500
Application Notes
This EGLN1/PHD2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplamic and nuclear staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
EGLN1/PHD2 Overexpression Lysate
Reviewed Applications
Read 12 Reviews rated 4.8
using
NB100-137 in the following applications:

Publications
Read Publications using
NB100-137 in the following applications:

  • 5 publications
  • IHC
    6 publications
  • 4 publications
  • IP
    5 publications
  • WB
    52 publications

Reactivity Notes

Results for use of this EGLN1/PHD2 antibody have been mixed in Rat with success in Western blot analysis and immunofluorescence on Rat endothelial cells and negative results with PC12 cells. Mouse reactivity reported in scientific literature (PMID: 25578858). Rat reactivity reported in scientific literature (PMID: 25635047). Primate reactivity reported in scientific literature (PMID: 25974097)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7 to 8)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EGLN1/PHD2 Antibody

  • C1orf12
  • EC 1.14.11.29
  • EC:1.14.11.291
  • ECYT3
  • egl nine homolog 1
  • egl nine-like protein 1
  • EGLN1
  • HALAH
  • HIF prolyl hydroxylase 2
  • HIFPH2
  • HIF-PH2
  • HIF-prolyl hydroxylase 2
  • HPH2
  • HPH-2
  • Hypoxia-inducible factor prolyl hydroxylase 2
  • PHD2
  • PNAS-118
  • PNAS-137
  • Prolyl hydroxylase domain-containing protein 2
  • SM20
  • SM-20
  • zinc finger MYND domain-containing protein 6
  • ZMYND6

Background

PHD2 (Prolyl Hydroxylase Domain-containing protein 2) belongs to the Prolyl-4-hydroxylase domain (PHD) family of proteins and is encoded by the Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1) gene (1). Human EGLN1/PHD2 is a ubiquitously expressed enzyme that is 426 amino acids (aa) long with a theoretical molecular weight of ~46 kDa. Structurally PHD2 contains a nuclear export signal (NES, aa 6-20), an N-terminal MYND zinc finger domain (aa 21-58), and a C-terminal catalytic domain (aa 291-392) (2, 3). Functionally, PHD2 serves as an oxygen sensor and is responsible for post-translational modification of Hypoxia-inducible factor alpha (HIF-1alpha), a component of a transcriptional complex involved in oxygen homeostasis (1-3). During normoxia, PHD2 is responsible for oxygen-dependent hydroxylation of HIF-1alpha proline residue 402, 564, or both (3). The hydroxylation event promotes the binding of von Hippel-Lindau protein (VHL) and targets HIF1-alpha for ubiquitination and degradation (4, 5).

EGLN1/PHD2 has been implicated in several critical processes including erythropoiesis, angiogenesis, and metabolism as well as various pathologies such as cancer (2, 5, 6). Studies in mice have found that somatic deletion of PHD2 resulted in higher vascular endothelial growth factor A (VEGF-A) levels, increased blood vessel formation, and more erythropoietin (EPO), leading to severe polycythemia or erythrocytosis (high red blood cell (RBC) volume) (6). Another study revealed that specific point mutations in EGLN1/PHD2 led to elevated EPO and RBC mass associated with hemorrhages and strokes (6). Accordingly, given the known role of PHD2 in inhibition of EPO production, PHD2 inhibitors are being studied as a potential therapeutic for anemia (6). Additionally, dysregulation in EGLN1, and specifically the PHD2-VHL-HIF-1alpha pathway, has been associated with the development of pheochromocytomas (PCC) and sympathetic paragangliomas (PGL), which are rare neuroendocrine tumors (2). Besides pathological features, EGLN1/PHD2 may also be important for high altitude adaptation as two coding sequence variants in PHD2 are prevalent in the Tibetan population but is very rare in people at lower altitudes (2).

Alternate names for EGLN1/PHD2 include HIF Prolyl Hydroxylase 2, PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, egl nine homolog 1, and C1orf12.

References

1. Amorim-Pires, D., Peixoto, J., & Lima, J. (2016). Hypoxia Pathway Mutations in Pheochromocytomas and Paragangliomas. Cytogenetic and genome research. https://doi.org/10.1159/000457479

2. Gardie, B., Percy, M. J., Hoogewijs, D., Chowdhury, R., Bento, C., Arsenault, P. R., Richard, S., Almeida, H., Ewing, J., Lambert, F., McMullin, M. F., Schofield, C. J., & Lee, F. S. (2014). The role of PHD2 mutations in the pathogenesis of erythrocytosis. Hypoxia (Auckland, N.Z.). https://doi.org/10.2147/HP.S54455

3. Minervini, G., Quaglia, F., & Tosatto, S. C. (2015). Insights into the proline hydroxylase (PHD) family, molecular evolution and its impact on human health. Biochimie. https://doi.org/10.1016/j.biochi.2015.07.009

4. Semenza G. L. (2007). Hypoxia-inducible factor 1 (HIF-1) pathway. Science's STKE : signal transduction knowledge environment. https://doi.org/10.1126/stke.4072007cm8

5. Chan, D. A., & Giaccia, A. J. (2010). PHD2 in tumour angiogenesis. British journal of cancer. https://doi.org/10.1038/sj.bjc.6605682

6. Meneses, A. M., & Wielockx, B. (2016). PHD2: from hypoxia regulation to disease progression. Hypoxia (Auckland, N.Z.). https://doi.org/10.2147/HP.S53576

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGLN1/PHD2 Antibody (NB100-137)(83)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(5)
IHC
(6)
IHC-P
(4)
IP
(5)
WB
(52)
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Filter By Species
Human
(54)
Mouse
(7)
Rat
(7)
Primate
(1)
All Species
Showing Publications 1 - 10 of 83. Show All 83 Publications.
Publications using NB100-137 Applications Species
Jian CB, Yu XE, Gao HD et al. Liposomal PHD2 Inhibitors and the Enhanced Efficacy in Stabilizing HIF-1alpha Nanomaterials (Basel, Switzerland) Jan 3 2022 [PMID: 35010112] (WB, Human) WB Human
Li J, Lu X, Wei L Et al. PHD2 attenuates high-glucose-induced blood retinal barrier breakdown in human retinal microvascular endothelial cells by regulating the Hif-1 alpha/VEGF pathway Inflammation research : official journal of the European Histamine Research Society ... [et al.] Nov 13 2021 [PMID: 34773469] (WB, Human) WB Human
Kumar P, Verma V, Mohania D Et al. Leukemia associated RUNX1T1 gene reduced proliferation and invasiveness of glioblastoma cells Journal of cellular biochemistry Aug 9 2021 [PMID: 34369622]
Shi M, Dai WQ, Jia RR et al. APCCDC20-mediated degradation of PHD3 stabilizes HIF-1a and promotes tumorigenesis in hepatocellular carcinoma Cancer Lett Oct 9 2020 [PMID: 33039559] (IP, WB, Mouse) IP, WB Mouse
Mei H, Wu N, Huang X et al. Possible mechanisms by which silkworm faeces extract ameliorates adenine-induced renal anaemia in rats J Ethnopharmacol Oct 3 2020 [PMID: 33022342] (WB, Rat) WB Rat
Sundaramurthi, H, Roche, S L Et al. Selective Histone Deacetylase 6 Inhibitors Restore Cone Photoreceptor Vision or Outer Segment Morphology in Zebrafish and Mouse Models of Retinal Blindness. Front Cell Dev Biol Sep 29 2020 [PMID: 32984302] (IP, Mouse) IP Mouse
Settelmeier S, Schreiber T, MAki J et al. Prolyl hydroxylase domain 2 reduction enhances skeletal muscle tissue regeneration after soft tissue trauma in mice PLoS ONE May 15 2020 [PMID: 32413092] (IHC, Mouse) IHC Mouse
Dabral S, Muecke C, Valasarajan C et al. A RASSF1A-HIF1 alpha loop drives Warburg effect in cancer and pulmonary hypertension Nat Commun May 13 2019 [PMID: 31086178] (IP, Human) IP Human
Kurelac I, Iommarini L, Vatrinet R et al. Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses Nat Commun Feb 22 2019 [PMID: 30796225] (WB, Human) WB Human
Liu S, Zhang G, Guo J et al. Loss of Phd2 cooperates with BRAFV600E to drive melanomagenesis Nat Commun Dec 21 2018 [PMID: 30575721] (IHC, Human) IHC Human
Show All 83 Publications.

Reviews for EGLN1/PHD2 Antibody (NB100-137) (12) 4.812

Average Rating: 4.8
(Based on 12 reviews)
We have 12 reviews tested in 3 species: Human, Human and mouse liver cells, Other.

Reviews using NB100-137:
Filter by Applications
Immunofluorescence and WB
(1)
Western Blot
(10)
Immunofluorescence
(1)
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Filter by Species
Human
(9)
Human and mouse liver cells
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Other EGLN1/PHD2 NB100-137
Enlarge
5
reviewed by:
Kishor Pant
Immunofluorescence and WB Human and mouse liver cells 01/19/2020
View

Summary

ApplicationOther
Sample TestedProtein and paraffin sections
SpeciesHuman and mouse liver cells
LotA1
Western Blot EGLN1/PHD2 NB100-137
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 10/23/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman epithelial cell lines
SpeciesHuman
LotA3
Western Blot EGLN1/PHD2 NB100-137
Enlarge
5
reviewed by:
Veena Mysore
Western Blot Human 10/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedOPM2 cells whole cell lysate
SpeciesHuman
LotA3
Western Blot EGLN1/PHD2 NB100-137
Enlarge
5
reviewed by:
Prabir Chakraborty
Western Blot Human 06/30/2015
View

Summary

ApplicationWestern Blot
Sample TestedCell lysates from prostate cancer cell lines
SpeciesHuman
LotLot A3
  4
reviewed by:
Anonymous -
Western Blot Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23225569
SpeciesHuman
  4
reviewed by:
cynthia morimoto
Western Blot Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23328582
SpeciesHuman
  5
reviewed by:
Wenwen Sun
Western Blot Human 11/21/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
  5
reviewed by:
Ning Wang
Western Blot Other 09/22/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman SH-SY5Y or rat PC12 whole cell lysate.
SpeciesOther
LotA
  5
reviewed by:
Ning Wang
Western Blot Other 08/24/2014
View

Summary

ApplicationWestern Blot
Sample TestedSK-N-BE(2) whole cell lysate
SpeciesOther
LotA
Immunofluorescence EGLN1/PHD2 NB100-137
Enlarge
5
reviewed by:
Wenwen Sun
Immunofluorescence Human 04/28/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedU2OS (human osteosarcoma cell line)
SpeciesHuman
Western Blot EGLN1/PHD2 NB100-137
Enlarge
5
reviewed by:
Wenwen Sun
Western Blot Human 04/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedU87MG, U138MG, U343MG whole cell lysates
SpeciesHuman
  5
reviewed by:
Weibo Luo
Western Blot Human 07/08/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 20 ug
SpeciesHuman

Product General Protocols

View specific protocols for EGLN1/PHD2 Antibody (NB100-137): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGLN1/PHD2 Antibody (NB100-137). (Showing 1 - 2 of 2 FAQs).

  1. What is the probability of this antibody cross-reacting with the bovine protein. Can you send me the alignment score of the immunogen with the bovine protein?
    • The immunogen used to generate this antibody shares 100% homology with bovine. This antibody will likely cross react with bovine, however we have not yet tested it in that species. If you would be interested in testing this species, please take a look at our Innovators Reward Program.
  2. I would like to use this antibody but it has not been validated in my species of interest. Is there any way I can find out if it will work?
    • We offer risk-free testing of all of our primary antibodies. Please check out our Innovator's Reward Program and test this EGLN1-PHD2 antibody in any unvalidated species or application, without the financial risk of failure.

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Recent Reviews

4.8
5
10
4
2
3
0
2
0
1
0

Kishor Pant
01/19/2020
Application: Immunofluorescence and WB
Species: Human and mouse liver cells

Verified Customer
10/23/2017
Application: Western Blot
Species: Human

Veena Mysore
10/08/2015
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol EGLN1
Entrez
Uniprot