EGLN1/PHD2 Antibody

Images

 
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of Human PHD2 by Western Blot. Samples : Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells. Antibody : Affinity ...read more
Immunocytochemistry/ Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of endogenous PHD2 in U2OS cells. Image from verified customer review.
Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-137] - Staining of Vascular Endothelium 40X in Lung.
Flow Cytometry: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 NB100-137 in a 150 ul reaction. Isotype control (black), ...read more
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 expression in human prostate cancer cell lines. Image from verified customer review.
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in Hep3B cell lysate.
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2 human glioblastoma cells. Image from verified customer review.
Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in normoxia (lane 1) or hypoxia (lane 2) treated SK-N-BE(2) cells using anti-PHD2 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of PHD2 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: EGLN1/PHD2 Antibody [NB100-137] - Simple Western lane view shows a specific band for PHD2/HIF Prolyl Hydroxylase 2 in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

EGLN1/PHD2 Antibody Summary

Immunogen
The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
Localization
Cytoplasm, nucleus
Predicted Species
Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2500
  • Simple Western 1:500
  • Flow Cytometry 3.0 mcg/ml
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
Application Notes
This PHD2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplamic and nuclear staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
EGLN1/PHD2 Lysate (NBL1-10153)
Reviewed Applications
Read 9 Reviews rated 4.8
using
NB100-137 in the following applications:

Publications
Read Publications using
NB100-137 in the following applications:

  • 5 publications
  • IHC
    4 publications
  • 4 publications
  • IP
    2 publications
  • WB
    39 publications

Reactivity Notes

Results have been mixed in Rat with success in western analysis and immunofluorescence on Rat endothelial cells and negative results with PC12 cells. Expected reactivity with Rabbit, Orangutan, Rhesus Monkey, Gorilla based on 100% sequence identity.Mouse reactivity reported in scientific literature (PMID: 25578858) Rat reactivity reported in scientific literature (PMID: 25635047). Primate reactivity reported in scientific literature (PMID: 25974097)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EGLN1/PHD2 Antibody

  • C1orf12
  • C1orf12HPH2
  • EC 1.14.11
  • EC 1.14.11.-
  • ECYT3
  • egl nine homolog 1 (C. elegans)
  • egl nine homolog 1
  • egl nine-like protein 1
  • EGLN1
  • HIF prolyl hydroxylase 2
  • HIFPH2 EGL nine (C.elegans) homolog 1
  • HIFPH2
  • HIF-PH2
  • HIF-prolyl hydroxylase 2
  • HPH2
  • HPH-2
  • Hypoxia-inducible factor prolyl hydroxylase 2
  • PHD2
  • PHD2SM20
  • Prolyl hydroxylase domain-containing protein 2
  • SM20
  • SM-20
  • zinc finger MYND domain-containing protein 6
  • ZMYND6
  • ZMYND6DKFZp761F179

Background

PHD2/HIF Prolyl Hydroxylase 2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity. Alternate names for PHD2/HIF Prolyl Hydroxylase 2 include PHD2, PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, EGLN1, egl nine homolog 1, and C1orf12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-137
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP

Publications for EGLN1/PHD2 Antibody (NB100-137)(64)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(5)
IHC
(4)
IHC-P
(4)
IP
(2)
WB
(39)
All Applications
Filter By Species
Human
(45)
Mouse
(2)
Rat
(4)
Primate
(1)
All Species
Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using NB100-137 Applications Species
Miles AL, Burr SP, Grice GL, Nathan JA. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1a prolyl hydroxylation by regulating cellular iron levels. Elife. Mar 15 2017 [PMID: 28296633] (ICC/IF, Human) ICC/IF Human
Wottawa M, Naas S, Bottger J et al. Hypoxia-stimulated membrane trafficking requires T-plastin. Acta Physiol (Oxf) 2017 Feb 20 [PMID: 28218996]
Burr SP, Costa AS, Grice GL et al. Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1a Stability in Aerobic Conditions. Cell Metab. Nov 8 2016 [PMID: 27923773] (WB, Human) WB Human
Lam YT, Lecce L, Tan JT et al. Androgen Receptor-Mediated Genomic Androgen Action Augments Ischemia-Induced Neovascularization. Endocrinology 2016 Dec [PMID: 27754785]
Tao Y, Lin F, Li R et al. Prolyl hydroxylase-2 inhibits liver tumor cell proliferation and cyclin D1 expression in a hydroxylase-dependent manner. Int. J. Biochem. Cell Biol. Jun 13 2016 [PMID: 27307407] (WB, Human) WB Human
Mysore VS, Szablowski J, Dervan PB, Frost PJ. A DNA-binding Molecule Targeting the Adaptive Hypoxic Response in Multiple Myeloma Has Potent Antitumor Activity. Mol Cancer Res. [PMID: 26801054] (WB, Human) WB Human
Sun W, Kosyna FK, Jelkmann W, Depping R. Prolyl-4-Hydroxylase 2 Potentially Contributes to Hepatocellular Carcinoma-Associated Erythrocytosis by Maintaining Hepatocyte Nuclear Factor-4alpha Expression. Cell. Physiol. Biochem. 2015 Dec 24 [PMID: 26624507] (WB, Human) WB Human
Hogel H, Miikkulainen P, Bino L, Jaakkola PM. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27. Mol. Cancer. 2015 Jul 30 [PMID: 26223520] (WB, Human)

Details:
PHD2 antibody used for WB on 786-O cell under hypoxia (1% O2) and normoxia (21 % O2) by siPHD2 (Figure 1C).
WB Human
Ryou MG, Choudhury GR, Li W et al. Methylene blue-induced neuronal protective mechanism against hypoxia-reoxygenation stress Neuroscience. 2015 Jun 03 [PMID: 26047733] (WB, Human) WB Human
Zhang Z, Pang X, Tang Z et al. Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015 Sep [PMID: 25975603]
Show All 64 Publications.

Reviews for EGLN1/PHD2 Antibody (NB100-137) (9) 4.89

Average Rating: 4.8
(Based on 9 reviews)
We have 9 reviews tested in 1 species: Human.
We have 9 reviews tested in 2 application: WB, IF.

Reviews using NB100-137:
Filter by Applications
WB
(8)
IF
(1)
All Applications
Filter by Species
Human
(9)
All Species
Images Ratings Applications Species Date Details
Western Blot EGLN1/PHD2 NB100-137
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reviewed by:
John Collins
WB Human 10/23/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman epithelial cell lines
SpeciesHuman
LotA3
Western Blot EGLN1/PHD2 NB100-137
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reviewed by:
Anonymous
WB Human 10/08/2015
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Summary

ApplicationWestern Blot
Sample TestedOPM2 cells whole cell lysate
SpeciesHuman
LotA3

Blocking

Blocking DetailsPBST 0.2%Tween 20, 5% non fat milk, 30 minutes at Room Temperature

Primary Anitbody

Dilution Ratio1:1000, Overnight, 5 C, PBST with 5%milk

Secondary Antibody

Secondary DescriptionGoat anti-rabbit igG HRP
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:2000

Details

Detection NotesChemiluminescence, G:Box Chemi XLT4, 20 sec, no positive and negative controls

Comments

CommentsSemi-dry transfer method, used 4-12% Nupage gels from Life technologies
Western Blot EGLN1/PHD2 NB100-137
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reviewed by:
Anonymous
WB Human 06/30/2015
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Summary

ApplicationWestern Blot
Sample TestedCell lysates from prostate cancer cell lines
SpeciesHuman
LotLot A3

Blocking

Blocking Details4% BSA in TBST for 1h at room temperature

Primary Anitbody

Dilution Ratio1:500 in TBST + 1%BSA, overnight, 4*C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:10,000

Details

Detection NotesECL method using Femto reagent
 
reviewed by:
Anonymous
WB Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23225569
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23225569

Details

Detection NotesSee PMID 23225569

Comments

CommentsPublished PMID 23225569
 
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23328582
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23328582

Details

Detection NotesSee PMID 23328582

Comments

CommentsPublished in PMID: 23328582Independent review from antibodyresource.com
 
reviewed by:
Anonymous
WB Human 11/21/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF
Immunofluorescence EGLN1/PHD2 NB100-137
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reviewed by:
Anonymous
IF Human 04/28/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedU2OS (human osteosarcoma cell line)
SpeciesHuman

Blocking

Blocking Details4% FSG (fish skin gelatin)/PBS, 4 degrees celsius, over night

Primary Anitbody

Dilution Ratio1:100 in 2% FSG/PBS, 4 degrees celsius, over night

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, Alexa594
Secondary Manufacturer Cat#Invitrogen
Secondary Concentration1:800

Details

Detection NotesFluorescence microscope (Zeiss, Axioplan)
Fixation Details15Min room temperature 3.7% Formaldehyde, 10Min room temperature 0.1% Triton X-100
Wash DescriptionPBS, 5Min, three times
Western Blot EGLN1/PHD2 NB100-137
Enlarge
reviewed by:
Anonymous
WB Human 04/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedU87MG, U138MG, U343MG whole cell lysates
SpeciesHuman

Blocking

Blocking Details1h, 3% milk/PBS, room temperature

Primary Anitbody

Dilution Ratio2h, 1:500 in 3% milk/PBS, room temperature

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Dako
Secondary Concentration1:2000

Details

Detection NotesECL, X-ray film, 2 Min exposure
 
reviewed by:
Anonymous
WB Human 07/08/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 20 ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit IgG-HRP, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 46 kDa, Non-Specific Bands: 70, 80, 120 kDa

Product General Protocols

View specific protocols for EGLN1/PHD2 Antibody (NB100-137): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGLN1/PHD2 Antibody (NB100-137). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know the probability of this antibody crossreacting with the bovine protein. Can you send me the alignment score of the immunogen with the bovine protein?
    • The immunogen used to generate this antibody shares 100% homology with bovine. This antibody will likely cross react with bovine, however we have not yet tested it in that species. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-137AF405
Alexa Fluor 488 Labeled NB100-137AF488
Alexa Fluor 647 Labeled NB100-137AF647
Alexa Fluor 700 Labeled NB100-137AF700
Biotin Labeled NB100-137B
DyLight 405 Labeled NB100-137V
DyLight 488 Labeled NB100-137G
DyLight 550 Labeled NB100-137R
DyLight 650 Labeled NB100-137C
FITC Labeled NB100-137F
HRP Labeled NB100-137H
PE Labeled NB100-137PE

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Recent Reviews

4.8
5
7
4
2
3
0
2
0
1
0

John Collins
10/23/2017
Application: WB
Species: Human

Anonymous
10/08/2015
Application: WB
Species: Human

Anonymous
06/30/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol EGLN1
Entrez
Uniprot