AKT [p Ser473] Antibody - Pan Specific

Images

 
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated ...read more
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 ...read more
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

AKT [p Ser473] Antibody - Pan Specific Summary

Immunogen
Phosphopeptide containing human, mouse, and rat Akt1 S473 site
Modification
p Ser473
Specificity
Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKT1
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF887 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen and protein A Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AKT [p Ser473] Antibody - Pan Specific

  • RAC-ALPHA
  • AKT serine/threonine kinase 1
  • Akt
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC

Background

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKT Antibody (AF887)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC - Frozen, IHC Paraffin-embedded, WB.


Filter By Application
IHC - Frozen
(1)
IHC Paraffin-embedded
(1)
WB
(17)
All Applications
Filter By Species
Human
(13)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF887 Applications Species
J Xiao, ME Egger, KM McMasters, H Hao Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines BMC Cancer, 2018;18(1):675. 2018 [PMID: 29929490] (WB,, Human) WB Human
D Belloni, S Heltai, M Ponzoni, A Villa, B Vergani, L Pecciarini, M Marcatti, S Girlanda, G Tonon, F Ciceri, F Caligaris-, M Ferrarini, E Ferrero Modeling Multiple Myeloma-Bone Marrow interactions and response to drugs in a 3D surrogate microenvironment Haematologica, 2018;0(0):. 2018 [PMID: 29326121] (WB,, Human) WB Human
DL Fischer, CJ Kemp, A Cole-Strau, NK Polinski, KL Paumier, JW Lipton, K Steece-Col, TJ Collier, DJ Buhlinger, CE Sortwell Subthalamic Nucleus Deep Brain Stimulation Employs trkB Signaling for Neuroprotection and Functional Restoration J. Neurosci., 2017;37(28):6786-6796. 2017 [PMID: 28607168] (IHC - Frozen,, Mouse) IHC - Frozen Mouse
MM Budai, J T?zsér, S Benk? Different dynamics of NLRP3 inflammasome-mediated IL?1? production in GM-CSF- and M-CSF-differentiated human macrophages J. Leukoc. Biol, 2017;0(0):. 2017 [PMID: 28232386] (WB,, Human) WB Human
Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents Invest. Ophthalmol. Vis. Sci, 2016;57(15):6683-6691. 2016 [PMID: 27936470] (WB,, Mouse) WB Mouse
Divakar S, Ramana Reddy M, Cosenza S, Baker S, Perumal D, Antonelli A, Brody J, Akula B, Parekh S, Premkumar Reddy E Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Leukemia, 2016;30(1):86-93. 2016 [PMID: 26174628] (WB,, Human) WB Human
Dicay M, Hirota C, Ronaghan N, Peplowski M, Zaheer R, Carati C, MacNaughton W Interferon-gamma suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation. PLoS ONE, 2015;10(3):e0118713. 2015 [PMID: 25793528] (WB,, Mouse) WB Mouse
Marti H, Koschwanez M, Pesch T, Blenn C, Borel N Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection. PLoS ONE, 2014;9(7):e102239. 2014 [PMID: 25019934] (WB,, Human) WB Human
von Roemeling C, Radisky D, Marlow L, Cooper S, Grebe S, Anastasiadis P, Tun H, Copland J Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4. Cancer Res, 2014;74(17):4796-810. 2014 [PMID: 24962026] (WB,, Human) WB Human
Mao Y, Xu J, Li Z, Zhang N, Yin H, Liu Z The role of nuclear beta-catenin accumulation in the Twist2-induced ovarian cancer EMT. PLoS ONE, 2013;8(11):e78200. 2013 [PMID: 24244294] (WB,, Human) WB Human
Show All 19 Publications.

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Product General Protocols

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Video Protocols

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Blogs on AKT. Showing 1-10 of 26 blog posts - Show all blog posts.

Novel Insights into Hypoxia Induced AKT Signaling
Hypoxia is a common feature of most tumors and is a product of rapid cell growth and poor vascularization1. When oxygen availability is low in the tumor environment, the hypoxia inducing transcription factors (HIFs) regulate a variety of signaling...  Read full blog post.

Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy
Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...  Read full blog post.

The role of LC3B and autophagy in alcohol induced liver disease
Autophagy is a crucial intracellular pathway that manages the degradation and recycling of long-lived proteins in the cell. The LC3 (or light chain 3) family is composed of three members, LC3A, LC3B and LC3C. Upon autophagy induction, LC3 is cleave...  Read full blog post.

Showing 1-10 of 26 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol AKT1