AKT [p Ser473] Antibody [Unconjugated] - Pan Specific


Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (120-HD) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with ...read more
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody ...read more
Serum-starved MCF-7 human breast cancer cells were unstimulated (blue open histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained ...read more
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (291-G1) for 15 minutes, loaded ...read more
Phospho-proteome profiling results of LX-2 cells co-cultured with C3A–CYP2E1 cells with or without DDC treatment and detection of the effect of DDC on intracellular kinases in LX-2 cells of co-cultures200 μg of ...read more
Phospho-proteome profiling results of LX-2 cells co-cultured with C3A–CYP2E1 cells with or without DDC treatment and detection of the effect of DDC on intracellular kinases in LX-2 cells of co-cultures200 μg of ...read more
DDC up-regulates MMP-1 through ERK1/2 and Akt activation(A) Co-cultures were treated with 100 μM DDC for the indicated time. Phosphorylation of ERK1/2, p38 and Akt were determined by Western blotting analysis. The ...read more
Enhancement of autophagy by SLAMF5 is not mediated by inhibition of the mTORC1 complex. (A) The autophagy-inducer rapamycin was applied to control or SLAMF5-silenced cells for 4 h. Autophagy was monitored by western ...read more
p38 inhibitor SB203580 improves the up-regulation of MMP-1 by DDC through stimulating ERK1/2Co-cultures were treated with or without 100 μM DDC for 1 h in the presence or absence of p38 inhibitor (SB203580, 10 μM). ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow

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AKT [p Ser473] Antibody [Unconjugated] - Pan Specific Summary

Phosphopeptide containing human, mouse, and rat Akt1 S473 site
p Ser473
Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
AF887 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

  • AKT serine/threonine kinase 1
  • Akt
  • PKB
  • RAC


Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKT Antibody (AF887)(45)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Primate,  Human.

We have publications tested in 6 applications: Bioassay, ELISA Development, IHC, IHC-Fr, IHC-P, Western Blot.

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ELISA Development
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Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using AF887 Applications Species
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
Zhang, FW;Xie, XW;Chen, MH;Tong, J;Chen, QQ;Feng, J;Chen, FT;Liu, WQ; Poly(A)-specific ribonuclease protein promotes the proliferation, invasion and migration of esophageal cancer cells World journal of gastroenterology 2023-08-21 [PMID: 37664151] (Western Blot, Human) Western Blot  Human
Festa J, Hussain A, Hackney A et al. Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis Food Science & Nutrition 2023-07-01 [PMID: 37457144]
Noor Tarawneh, Lama Hamadneh, Bashaer Abu-Irmaileh, Ziad Shraideh, Yasser Bustanji, Shtaywy Abdalla Berberine Inhibited Growth and Migration of Human Colon Cancer Cell Lines by Increasing Phosphatase and Tensin and Inhibiting Aquaporins 1, 3 and 5 Expressions Molecules 2023-04-29 [PMID: 37175233]
Sharanya Nag, Sayanur Rahaman, Abhishek Guha, Partho Sarothi Ray An AKT1-and TRIM21-mediated phosphodegron controls proteasomal degradation of HuR enabling cell survival under heat shock iScience 2023-04-21 [PMID: 36968077]
OE Mohamed, DM Abdallah, AM Fayez, RA Mohamed, HS El-Abhar Morin post-treatment surpassed calpeptin in ameliorating 3-NP-induced cortical neurotoxicity via modulation of glutamate/calpain axis, Kidins220, and BDNF/TrkB/AKT/CREB trajectory International immunopharmacology, 2023-02-01;116(0):109771. 2023-02-01 [PMID: 36736222] (Western Blot, Rat) Western Blot Rat
OY Teran, MR Zanotelli, MJ Lin, RA Cerione, KF Wilson Dock7 regulates AKT and mTOR/S6K activity required for the transformed phenotypes and survival of cancer cells bioRxiv : the preprint server for biology, 2023-01-03;0(0):. 2023-01-03 [PMID: 36711811] (Bioassay, N/A) Bioassay N/A
Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells Pharmaceuticals (Basel) 2022-09-08 [PMID: 36145344]
JH Hwang, SY Kang, HW Jung Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway Molecular Medicine Reports, 2022-04-08;25(6):. 2022-04-08 [PMID: 35419614] (Western Blot, Mouse) Western Blot Mouse
M Mortoglou, L Mani?, A Buha Djord, Z Bulat, V ?or?evi?, K Manis, E Valle, L York, D Wallace, P Uysal-Onga Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs Toxics, 2022-03-21;10(3):. 2022-03-21 [PMID: 35324773] (ELISA Development, Human) ELISA Development Human
Show All 45 Publications.

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Secondary Antibodies


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Additional AKT Products

Blogs on AKT. Showing 1-10 of 29 blog posts - Show all blog posts.

Insulin signaling in adipocytes: Carbohydrate-signaling transcription factor ChREBP is the link between lipolytic enzyme Hormone-Sensitive Lipase and lipogenic enzyme ELOVL6
By Jamshed Arslan, Pharm. D., PhD. Insulin resistance in adipocytes is a major feature of metabolic syndrome   . Disrupted adipose tissue metabolism can lead to accumulation of lipid intermediates in insul...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Novel Insights into Hypoxia Induced AKT Signaling
Hypoxia is a common feature of most tumors and is a product of rapid cell growth and poor vascularization1. When oxygen availability is low in the tumor environment, the hypoxia inducing transcription factors (HIFs) regulate a variety of signaling ...  Read full blog post.

Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1, ...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

Showing 1-10 of 29 blog posts - Show all blog posts.
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Gene Symbol AKT1